FvH4_6g42490

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001118
Physical Location & Seq
Reverse (-)
33106250 .. 33107062
813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42490.t1

Sequence Viewer

Length: 531 bp
ATGGCATTAACCATATCCCCACTGCTCATTTTGGTTCTGAGTACTACTGTTTTAGCCATGGCCAGTAGTACTCATCATGAGTTGAAAGAAACCAAAATGTCTATGTACATGCACGACTTCAGCGCCGGTCCAAACATCACGGATATACCAGTTGCTGGTATTGCCGGAAAGCTTTGGGTGTTTAACCAATTTGGCACAGTTTACGTCGTTGACGACTCCTTAACCGAAGGCCCGAGCCGTAATTCGACAGTAGTTGGCCGAGCACAGGGTATTGTTGTAACAGCATCACTAGACGGACGTAACGCCCTAGTTTTGCTGTCGATTGTGTTCACAAACAAAAAGTACAATGGCAGCACTATTGAGATTCAAGGAAACAGTAAGCAATTTGAGCCGATAAGAGAGGTGGCTGTGGTTTCTGGTACTGGTAAGTTTCGGTTTGCTAGAGGGTATGCTACGTTTGAAACTTACTTCGTCGACCAGCCAACTGATTACGCTGTTATACGCTCCAATATTACAGTGCAACACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

177

Amino Acids

19.24

Weight (kDa)

6.91

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 31 - 173 1.2e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 155
AccI GTMKAC 1 cut(s) 474
AcoI YGGCCR 2 cut(s) 60, 256
AcuI CTGAAG 1 cut(s) 103
AfaI GTAC 5 cut(s) 43, 70, 107, 344, 421
AfiI CCNNNNNNNGG 2 cut(s) 155, 265
AgsI TTSAA 3 cut(s) 85, 368, 461
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw21I GWGCWC 1 cut(s) 265
AlwNI CAGNNNCTG 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 3 cut(s) 60, 229, 256
ApeKI GCWGC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 128, 230
AvaI CYCGRG 1 cut(s) 232
AvaII GGWCC 1 cut(s) 128
BalI TGGCCA 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 363
BceAI ACGGC 1 cut(s) 222
BfaI CTAG 3 cut(s) 290, 308, 441
BfoI RGCGCY 1 cut(s) 126
BisI GCNGC 1 cut(s) 352
BlsI GCNGC 1 cut(s) 353
BmcAI AGTACT 2 cut(s) 43, 70
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 2 cut(s) 128, 230
BmsI GCATC 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 57
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 265
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 63, 149, 427
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 2 cut(s) 155, 265
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 3 cut(s) 63, 149, 427
BseXI GCAGC 1 cut(s) 363
BshFI GGCC 3 cut(s) 62, 231, 258
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 2 cut(s) 126, 165
BslI CCNNNNNNNGG 2 cut(s) 155, 265
BsnI GGCC 3 cut(s) 62, 231, 258
BsoBI CYCGRG 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 105
Bsp19I CCATGG 1 cut(s) 57
BspANI GGCC 3 cut(s) 62, 231, 258
BspCNI CTCAG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 125
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 3 cut(s) 63, 149, 427
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 57
Bst4CI ACNGT 5 cut(s) 49, 199, 250, 377, 517
BstAUI TGTACA 1 cut(s) 105
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstMWI GCNNNNNNNGC 2 cut(s) 161, 388
BstNSI RCATGY 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 363
BsuRI GGCC 3 cut(s) 62, 231, 258
BtgI CCRYGG 1 cut(s) 57
BtsI GCAGTG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 20, 522
CaiI CAGNNNCTG 1 cut(s) 155
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 128, 230
Csp6I GTAC 5 cut(s) 42, 69, 106, 343, 420
CviAII CATG 3 cut(s) 58, 77, 109
CviJI RGCY 9 cut(s) 56, 62, 172, 231, 237, 258, 391, 407, 481
CviKI_1 RGCY 9 cut(s) 56, 62, 172, 231, 237, 258, 391, 407, 481
CviQI GTAC 5 cut(s) 42, 69, 106, 343, 420
DdeI CTNAG 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 60, 256
Eco130I CCWWGG 1 cut(s) 57
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 57
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 3 cut(s) 61, 80, 112
FaiI YATR 8 cut(s) 14, 59, 78, 104, 110, 146, 450, 500
FatI CATG 3 cut(s) 57, 76, 108
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 352
FspBI CTAG 3 cut(s) 290, 308, 441
GlaI GCGC 1 cut(s) 124
GluI GCNGC 1 cut(s) 352
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 3 cut(s) 62, 231, 258
HapII CCGG 2 cut(s) 126, 165
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 61, 80, 112
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 2 cut(s) 211, 475
HindII GTYRAC 2 cut(s) 211, 475
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 215, 364
HpaII CCGG 2 cut(s) 126, 165
Hpy166II GTNNAC 4 cut(s) 202, 211, 330, 475
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 4 cut(s) 202, 211, 330, 475
Hpy99I CGWCG 2 cut(s) 209, 476
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 5 cut(s) 49, 199, 250, 377, 517
HpyCH4IV ACGT 3 cut(s) 204, 298, 455
HpyCH4V TGCA 2 cut(s) 112, 520
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 388
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 3 cut(s) 204, 298, 455
Hsp92II CATG 3 cut(s) 61, 80, 112
HspAI GCGC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 509
LpnPI CCDG 9 cut(s) 76, 139, 141, 162, 178, 251, 402, 408, 491
Lsp1109I GCAGC 1 cut(s) 363
LweI GCATC 1 cut(s) 293
MaeI CTAG 3 cut(s) 290, 308, 441
MaeII ACGT 3 cut(s) 204, 298, 455
MaeIII GTNAC 2 cut(s) 277, 299
MhlI GDGCHC 1 cut(s) 265
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 3 cut(s) 188, 241, 383
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 2 cut(s) 394, 437
Mox20I TGGCCA 1 cut(s) 62
MscI TGGCCA 1 cut(s) 62
MseI TTAA 3 cut(s) 8, 183, 221
Msp20I TGGCCA 1 cut(s) 62
MspI CCGG 2 cut(s) 126, 165
MwoI GCNNNNNNNGC 2 cut(s) 161, 388
NcoI CCATGG 1 cut(s) 57
NlaIII CATG 3 cut(s) 61, 80, 112
NmeAIII GCCGAG 1 cut(s) 284
NspI RCATGY 1 cut(s) 112
PagI TCATGA 1 cut(s) 76
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 1 cut(s) 364
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 1 cut(s) 353
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PspPI GGNCC 2 cut(s) 128, 230
PstNI CAGNNNCTG 1 cut(s) 155
RsaI GTAC 5 cut(s) 43, 70, 107, 344, 421
RsaNI GTAC 5 cut(s) 42, 69, 106, 343, 420
SalI GTCGAC 1 cut(s) 473
SaqAI TTAA 3 cut(s) 8, 183, 221
SatI GCNGC 1 cut(s) 352
Sau96I GGNCC 2 cut(s) 128, 230
ScaI AGTACT 2 cut(s) 43, 70
SchI GAGTC 1 cut(s) 209
SduI GDGCHC 1 cut(s) 265
SetI ASST 5 cut(s) 174, 207, 301, 405, 458
SfaNI GCATC 1 cut(s) 293
SinI GGWCC 1 cut(s) 128
Sse9I AATT 3 cut(s) 188, 241, 383
SspI AATATT 1 cut(s) 511
SspMI CTAG 3 cut(s) 290, 308, 441
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 5 cut(s) 49, 199, 250, 377, 517
TaiI ACGT 3 cut(s) 207, 301, 458
TaqI TCGA 3 cut(s) 245, 320, 474
TasI AATT 3 cut(s) 188, 241, 383
TatI WGTACW 4 cut(s) 41, 68, 105, 342
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 3 cut(s) 8, 183, 221
Tru9I TTAA 3 cut(s) 8, 183, 221
TscAI CASTG 2 cut(s) 27, 522
TseI GCWGC 1 cut(s) 351
TspGWI ACGGA 2 cut(s) 155, 309
TspRI CASTG 2 cut(s) 27, 522
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 128
XceI RCATGY 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 3 cut(s) 290, 308, 441
ZrmI AGTACT 2 cut(s) 43, 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.