Rh2BG547700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001118
Physical Location & Seq
Reverse (-)
75703161 .. 75703886
726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG547700.1

Sequence Viewer

Length: 522 bp
ATGGCATTAGCCATATCCCCACTGTTCATTTTGGTTCTGAGTACTTTTTTAGCCATGGCCAGTACTCGTGACTTCAAACAAACCCAACTGTCCATGTACTTACACGACTTCACCGCCGGTCCAAATCTCACGGGATTACCAATTATAGGTATTGCCGGCAAGCTTTGGACCTTTGCTCAATTTGGGACAGTTTACGTCAACGACGACCCCATAACCGAAGGCCCAAGCCCTACATCGGCAGTAGTTGGCCGAGCACAGGCTCTTTCTGTAACATCAGCACTAGACGGACAAAACGCCTTGGTCTTTTTGTCGATTGTGTTCACAAACAAGAAGTACAATGGCAGCACTATTGAGATACAAGGAAGCTATAAGCAATTTGAGCCGAGCACAGAGGTGGCGGTGGTTTCAGGTACTGGTAAATTTCGGTTTGCTAGGGGATATGCTACGTTCGAAACTTATTTCGTGGACCAACCAAATAGCTACTCTGTTATACGCTGCAATATTACAGTGCAGCACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

173

Amino Acids

18.86

Weight (kDa)

6.71

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 28 - 170 1.5e-44 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 114, 398
AcoI YGGCCR 2 cut(s) 57, 247
AcsI RAATTY 1 cut(s) 419
AfaI GTAC 5 cut(s) 43, 64, 98, 335, 412
AfiI CCNNNNNNNGG 3 cut(s) 146, 235, 256
AgsI TTSAA 1 cut(s) 76
AleI CACNNNNGTG 1 cut(s) 392
AluBI AGCT 3 cut(s) 163, 366, 480
AluI AGCT 3 cut(s) 163, 366, 480
Alw21I GWGCWC 2 cut(s) 256, 389
AlwNI CAGNNNCTG 1 cut(s) 413
AoxI GGCC 3 cut(s) 57, 220, 247
ApeKI GCWGC 3 cut(s) 342, 495, 511
ApoI RAATTY 1 cut(s) 419
AspS9I GGNCC 4 cut(s) 119, 168, 221, 466
AsuHPI GGTGA 1 cut(s) 103
AsuII TTCGAA 1 cut(s) 450
AvaII GGWCC 3 cut(s) 119, 168, 466
BalI TGGCCA 1 cut(s) 59
BauI CACGAG 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 256, 389
BbvI GCAGC 2 cut(s) 354, 482
BfaI CTAG 2 cut(s) 281, 432
BisI GCNGC 3 cut(s) 343, 496, 512
BlsI GCNGC 3 cut(s) 344, 497, 513
BmcAI AGTACT 2 cut(s) 43, 64
Bme18I GGWCC 3 cut(s) 119, 168, 466
BmgT120I GGNCC 4 cut(s) 119, 168, 221, 466
Bpu14I TTCGAA 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 54, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 235, 256
Bse118I RCCGGY 2 cut(s) 116, 155
Bse1I ACTGG 2 cut(s) 60, 418
BseDI CCNNGG 2 cut(s) 54, 297
BseLI CCNNNNNNNGG 3 cut(s) 146, 235, 256
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 2 cut(s) 60, 418
BseXI GCAGC 2 cut(s) 354, 482
BshFI GGCC 3 cut(s) 59, 222, 249
BsiHKAI GWGCWC 2 cut(s) 256, 389
BsiSI CCGG 2 cut(s) 117, 156
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 3 cut(s) 146, 235, 256
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 3 cut(s) 59, 222, 249
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 2 cut(s) 256, 389
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 2 cut(s) 114, 398
BspANI GGCC 3 cut(s) 59, 222, 249
BspCNI CTCAG 1 cut(s) 30
BspT104I TTCGAA 1 cut(s) 450
BsrFI RCCGGY 2 cut(s) 116, 155
BsrI ACTGG 2 cut(s) 60, 418
BssAI RCCGGY 2 cut(s) 116, 155
BssECI CCNNGG 2 cut(s) 54, 297
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 54, 297
Bst2BI CACGAG 1 cut(s) 66
Bst4CI ACNGT 4 cut(s) 24, 90, 190, 508
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 2 cut(s) 157, 161
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 379
BstV1I GCAGC 2 cut(s) 354, 482
BsuRI GGCC 3 cut(s) 59, 222, 249
BtgI CCRYGG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 20, 513
Cac8I GCNNGC 2 cut(s) 157, 161
CaiI CAGNNNCTG 1 cut(s) 413
Cfr10I RCCGGY 2 cut(s) 116, 155
Cfr13I GGNCC 4 cut(s) 119, 168, 221, 466
Csp6I GTAC 5 cut(s) 42, 63, 97, 334, 411
CviAII CATG 2 cut(s) 55, 94
CviQI GTAC 5 cut(s) 42, 63, 97, 334, 411
DdeI CTNAG 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 57, 247
Eco130I CCWWGG 2 cut(s) 54, 297
Eco47I GGWCC 3 cut(s) 119, 168, 466
EcoT14I CCWWGG 2 cut(s) 54, 297
ErhI CCWWGG 2 cut(s) 54, 297
FaeI CATG 2 cut(s) 58, 97
FaiI YATR 8 cut(s) 14, 56, 95, 146, 212, 369, 441, 491
FaqI GGGAC 1 cut(s) 199
FatI CATG 2 cut(s) 54, 93
Fnu4HI GCNGC 3 cut(s) 343, 496, 512
Fsp4HI GCNGC 3 cut(s) 343, 496, 512
FspBI CTAG 2 cut(s) 281, 432
GluI GCNGC 3 cut(s) 343, 496, 512
HaeIII GGCC 3 cut(s) 59, 222, 249
HapII CCGG 2 cut(s) 117, 156
Hin1II CATG 2 cut(s) 58, 97
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 161
HpaII CCGG 2 cut(s) 117, 156
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 4 cut(s) 193, 199, 321, 466
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 68
Hpy8I GTNNAC 4 cut(s) 193, 199, 321, 466
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 4 cut(s) 24, 90, 190, 508
HpyCH4IV ACGT 2 cut(s) 195, 446
HpyCH4V TGCA 2 cut(s) 498, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 379
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 2 cut(s) 195, 446
Hsp92II CATG 2 cut(s) 58, 97
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
LpnPI CCDG 6 cut(s) 73, 130, 169, 242, 393, 399
Lsp1109I GCAGC 2 cut(s) 354, 482
MaeI CTAG 2 cut(s) 281, 432
MaeII ACGT 2 cut(s) 195, 446
MaeIII GTNAC 2 cut(s) 68, 268
MhlI GDGCHC 2 cut(s) 256, 389
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 4 cut(s) 141, 179, 374, 419
MluNI TGGCCA 1 cut(s) 59
MnlI CCTC 1 cut(s) 385
Mox20I TGGCCA 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 1 cut(s) 59
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 2 cut(s) 117, 156
MwoI GCNNNNNNNGC 1 cut(s) 379
NaeI GCCGGC 1 cut(s) 157
NcoI CCATGG 1 cut(s) 54
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 2 cut(s) 58, 97
NmeAIII GCCGAG 2 cut(s) 275, 408
NmuCI GTSAC 1 cut(s) 68
NspV TTCGAA 1 cut(s) 450
OliI CACNNNNGTG 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 157
PkrI GCNGC 3 cut(s) 344, 497, 513
PspPI GGNCC 4 cut(s) 119, 168, 221, 466
PstNI CAGNNNCTG 1 cut(s) 413
RsaI GTAC 5 cut(s) 43, 64, 98, 335, 412
RsaNI GTAC 5 cut(s) 42, 63, 97, 334, 411
RseI CAYNNNNRTG 1 cut(s) 392
SatI GCNGC 3 cut(s) 343, 496, 512
Sau96I GGNCC 4 cut(s) 119, 168, 221, 466
ScaI AGTACT 2 cut(s) 43, 64
SduI GDGCHC 2 cut(s) 256, 389
SetI ASST 9 cut(s) 151, 165, 173, 198, 368, 396, 412, 449, 482
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 3 cut(s) 119, 168, 466
SmiMI CAYNNNNRTG 1 cut(s) 392
Sse9I AATT 4 cut(s) 141, 179, 374, 419
SsiI CCGC 2 cut(s) 114, 398
SspI AATATT 1 cut(s) 502
SspMI CTAG 2 cut(s) 281, 432
StyI CCWWGG 2 cut(s) 54, 297
TaaI ACNGT 4 cut(s) 24, 90, 190, 508
TaiI ACGT 2 cut(s) 198, 449
TaqI TCGA 2 cut(s) 311, 450
TasI AATT 4 cut(s) 141, 179, 374, 419
TatI WGTACW 4 cut(s) 41, 62, 96, 333
TscAI CASTG 2 cut(s) 27, 513
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 3 cut(s) 342, 495, 511
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 1 cut(s) 16
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 2 cut(s) 27, 513
VpaK11BI GGWCC 3 cut(s) 119, 168, 466
XapI RAATTY 1 cut(s) 419
XspI CTAG 2 cut(s) 281, 432
ZrmI AGTACT 2 cut(s) 43, 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.