Rh2CG518100

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001118
Physical Location & Seq
Reverse (-)
69279639 .. 69280376
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG518100.1

Sequence Viewer

Length: 525 bp
ATGGCATTAGCCATATCCCCACTGTTCATTTTGGTTCTGAGTACTTCTTTTTTAGCCATGGCCAGTACTCGTGACTTCAAACAAACCCAACTGTCCATGTACTTACACGACTTCACCGCCGGTCCAAATCTCACGGGATTACCGATTATAGGTATTGCCGGCAAGCTTTGGACCTTTGCTCAATTTGGGACAGTTTACGTCAACGACGACCCCATAACCGAAGGCCCAAGCCCTATATCGGCAGTAGTTGGCCGAGCACAGGCTCTTTCTGTAACATCAGCACTAGACGGACAAAACGCCTTGGTCTTTTTGTCGATTGTGTTCACAAACAAGAAGTACAATGGCAGCACTATTGAGATACAAGGAAGCTATAAGCAATTTGAGCCGAGCACAGAGGTGGCGGTGGTTTCAGGTACTGGTAAATTTCGGTTTGCTAGGGGATATGCTACGTTCGAAACTTATTTCGTGGACCAACCAAATAGCTACTCTGTTATACGCTGCAATATTACAGTGCAGCACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

174

Amino Acids

18.96

Weight (kDa)

6.71

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 29 - 171 8.2e-44 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 117, 401
AcoI YGGCCR 2 cut(s) 60, 250
AcsI RAATTY 1 cut(s) 422
AfaI GTAC 5 cut(s) 43, 67, 101, 338, 415
AfiI CCNNNNNNNGG 3 cut(s) 149, 238, 259
AgsI TTSAA 1 cut(s) 79
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 3 cut(s) 166, 369, 483
AluI AGCT 3 cut(s) 166, 369, 483
Alw21I GWGCWC 2 cut(s) 259, 392
AlwNI CAGNNNCTG 1 cut(s) 416
AoxI GGCC 3 cut(s) 60, 223, 250
ApeKI GCWGC 3 cut(s) 345, 498, 514
ApoI RAATTY 1 cut(s) 422
AspS9I GGNCC 4 cut(s) 122, 171, 224, 469
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 453
AvaII GGWCC 3 cut(s) 122, 171, 469
BalI TGGCCA 1 cut(s) 62
BauI CACGAG 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 259, 392
BbvI GCAGC 2 cut(s) 357, 485
BfaI CTAG 2 cut(s) 284, 435
BisI GCNGC 3 cut(s) 346, 499, 515
BlsI GCNGC 3 cut(s) 347, 500, 516
BmcAI AGTACT 2 cut(s) 43, 67
Bme18I GGWCC 3 cut(s) 122, 171, 469
BmgT120I GGNCC 4 cut(s) 122, 171, 224, 469
Bpu14I TTCGAA 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 57, 300
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 238, 259
Bse118I RCCGGY 2 cut(s) 119, 158
Bse1I ACTGG 2 cut(s) 63, 421
BseDI CCNNGG 2 cut(s) 57, 300
BseLI CCNNNNNNNGG 3 cut(s) 149, 238, 259
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 2 cut(s) 63, 421
BseXI GCAGC 2 cut(s) 357, 485
BshFI GGCC 3 cut(s) 62, 225, 252
BsiHKAI GWGCWC 2 cut(s) 259, 392
BsiSI CCGG 2 cut(s) 120, 159
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 3 cut(s) 149, 238, 259
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 3 cut(s) 62, 225, 252
Bsp119I TTCGAA 1 cut(s) 453
Bsp1286I GDGCHC 2 cut(s) 259, 392
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 2 cut(s) 117, 401
BspANI GGCC 3 cut(s) 62, 225, 252
BspCNI CTCAG 1 cut(s) 30
BspT104I TTCGAA 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 119, 158
BsrI ACTGG 2 cut(s) 63, 421
BssAI RCCGGY 2 cut(s) 119, 158
BssECI CCNNGG 2 cut(s) 57, 300
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 57, 300
Bst2BI CACGAG 1 cut(s) 69
Bst4CI ACNGT 4 cut(s) 24, 93, 193, 511
BstBI TTCGAA 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 160, 164
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 57
BstMWI GCNNNNNNNGC 1 cut(s) 382
BstV1I GCAGC 2 cut(s) 357, 485
BsuRI GGCC 3 cut(s) 62, 225, 252
BtgI CCRYGG 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 20, 516
Cac8I GCNNGC 2 cut(s) 160, 164
CaiI CAGNNNCTG 1 cut(s) 416
Cfr10I RCCGGY 2 cut(s) 119, 158
Cfr13I GGNCC 4 cut(s) 122, 171, 224, 469
Csp6I GTAC 5 cut(s) 42, 66, 100, 337, 414
CviAII CATG 2 cut(s) 58, 97
CviQI GTAC 5 cut(s) 42, 66, 100, 337, 414
DdeI CTNAG 1 cut(s) 38
EaeI YGGCCR 2 cut(s) 60, 250
Eco130I CCWWGG 2 cut(s) 57, 300
Eco47I GGWCC 3 cut(s) 122, 171, 469
EcoT14I CCWWGG 2 cut(s) 57, 300
ErhI CCWWGG 2 cut(s) 57, 300
FaeI CATG 2 cut(s) 61, 100
FaiI YATR 9 cut(s) 14, 59, 98, 149, 215, 236, 372, 444, 494
FaqI GGGAC 1 cut(s) 202
FatI CATG 2 cut(s) 57, 96
Fnu4HI GCNGC 3 cut(s) 346, 499, 515
Fsp4HI GCNGC 3 cut(s) 346, 499, 515
FspBI CTAG 2 cut(s) 284, 435
GluI GCNGC 3 cut(s) 346, 499, 515
HaeIII GGCC 3 cut(s) 62, 225, 252
HapII CCGG 2 cut(s) 120, 159
Hin1II CATG 2 cut(s) 61, 100
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 164
HpaII CCGG 2 cut(s) 120, 159
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 4 cut(s) 196, 202, 324, 469
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 4 cut(s) 196, 202, 324, 469
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 4 cut(s) 24, 93, 193, 511
HpyCH4IV ACGT 2 cut(s) 198, 449
HpyCH4V TGCA 2 cut(s) 501, 514
HpyF10VI GCNNNNNNNGC 1 cut(s) 382
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 2 cut(s) 198, 449
Hsp92II CATG 2 cut(s) 61, 100
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
LpnPI CCDG 6 cut(s) 76, 133, 172, 245, 396, 402
Lsp1109I GCAGC 2 cut(s) 357, 485
MaeI CTAG 2 cut(s) 284, 435
MaeII ACGT 2 cut(s) 198, 449
MaeIII GTNAC 2 cut(s) 71, 271
MhlI GDGCHC 2 cut(s) 259, 392
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 3 cut(s) 182, 377, 422
MluNI TGGCCA 1 cut(s) 62
MnlI CCTC 1 cut(s) 388
Mox20I TGGCCA 1 cut(s) 62
MroNI GCCGGC 1 cut(s) 158
MscI TGGCCA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 62
MspI CCGG 2 cut(s) 120, 159
MwoI GCNNNNNNNGC 1 cut(s) 382
NaeI GCCGGC 1 cut(s) 160
NcoI CCATGG 1 cut(s) 57
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 61, 100
NmeAIII GCCGAG 2 cut(s) 278, 411
NmuCI GTSAC 1 cut(s) 71
NspV TTCGAA 1 cut(s) 453
OliI CACNNNNGTG 1 cut(s) 395
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 204
PdiI GCCGGC 1 cut(s) 160
PkrI GCNGC 3 cut(s) 347, 500, 516
PspPI GGNCC 4 cut(s) 122, 171, 224, 469
PstNI CAGNNNCTG 1 cut(s) 416
RsaI GTAC 5 cut(s) 43, 67, 101, 338, 415
RsaNI GTAC 5 cut(s) 42, 66, 100, 337, 414
RseI CAYNNNNRTG 1 cut(s) 395
SatI GCNGC 3 cut(s) 346, 499, 515
Sau96I GGNCC 4 cut(s) 122, 171, 224, 469
ScaI AGTACT 2 cut(s) 43, 67
SduI GDGCHC 2 cut(s) 259, 392
SetI ASST 9 cut(s) 154, 168, 176, 201, 371, 399, 415, 452, 485
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 3 cut(s) 122, 171, 469
SmiMI CAYNNNNRTG 1 cut(s) 395
Sse9I AATT 3 cut(s) 182, 377, 422
SsiI CCGC 2 cut(s) 117, 401
SspI AATATT 1 cut(s) 505
SspMI CTAG 2 cut(s) 284, 435
StyI CCWWGG 2 cut(s) 57, 300
TaaI ACNGT 4 cut(s) 24, 93, 193, 511
TaiI ACGT 2 cut(s) 201, 452
TaqI TCGA 2 cut(s) 314, 453
TasI AATT 3 cut(s) 182, 377, 422
TatI WGTACW 4 cut(s) 41, 65, 99, 336
TscAI CASTG 2 cut(s) 27, 516
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 3 cut(s) 345, 498, 514
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 1 cut(s) 16
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 2 cut(s) 27, 516
VpaK11BI GGWCC 3 cut(s) 122, 171, 469
XapI RAATTY 1 cut(s) 422
XspI CTAG 2 cut(s) 284, 435
ZrmI AGTACT 2 cut(s) 43, 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.