RLG00000021121

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001118
Physical Location & Seq
Reverse (-)
72133092 .. 72134733
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021121

Sequence Viewer

Length: 804 bp
ATGGCATTACAGTTCAATTATCATTCCCTACTATTGGTCGTGGTTCTGGCCACGACTACATTCTTAGACATAGCCACTAGCAGTATAGAGTTGAAAGAAACCAAGCTATCCTTGTATTTCCAGGATTTCTCTGCCGGTCCAAATACCTCAGCAGTACCAATTGCAGGTATTGCAGGCAAGCTATGGAGGTTCGATCAGTTTGGAACAGTTATTGTCACGGATGACCGGCTAACTGTAGCCCCGAGTCCGAAATCAGCAACGGTGGGCCGAGGCCAAGGCTTGGTTGTAACATCATCACTAGATGGACGCAATGCCCATGTCTTGTTATCGATTGTGTTCACCAACAGGAGGTATAATGGCAGCACTCTAGAGATACAAGGAAATAGTAGGCAATTCGAGGCGGTTAGAGAGGTCTCGGTCGTATCAGGCACCGGGAAGTTTCGGTTTGCTAGAGGATATGCTACATTCGAAACTTATTCTGTGGACCCTACAAATGCATACTCAGTTATACGATTCAATATTACTCTGCAACACAAGATGCTGACGGAAACTAGTGGCAGGGTGCAATCTGCTGCGACCTATAGATTTGGAAGAGGATATGGCTCAAAGGTAGATGGGATGGATGCGAGGCTGGTTATGGCTGTGGGTAGACGGATTTTACAGGATCTGTTTTGGTGGAGATCTCGTAGTCTGGTTCGATTGGGTCGTTGGCTTTGGTGTGTTGGTGAGATGGTCAACCGGTGTGCTACGGCGATGGCGACTTCGGTGGATGTCAGTGGCCGCAGCGATTTCGATGGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

268

Amino Acids

29.55

Weight (kDa)

9.79

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 34 - 176 8.8e-44 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 428
AccB7I CCANNNNNTGG 1 cut(s) 280
AccI GTMKAC 1 cut(s) 649
AciI CCGC 2 cut(s) 401, 781
AclWI GGATC 1 cut(s) 672
AcoI YGGCCR 2 cut(s) 48, 778
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 4 cut(s) 34, 164, 280, 348
AgeI ACCGGT 1 cut(s) 738
AgsI TTSAA 3 cut(s) 16, 94, 517
AhlI ACTAGT 1 cut(s) 551
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 2 cut(s) 106, 181
AluI AGCT 2 cut(s) 106, 181
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 1 cut(s) 672
AlwNI CAGNNNCTG 1 cut(s) 667
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 4 cut(s) 48, 265, 271, 778
ApeKI GCWGC 3 cut(s) 360, 572, 783
AsiGI ACCGGT 1 cut(s) 738
AspS9I GGNCC 3 cut(s) 137, 265, 484
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 2 cut(s) 331, 737
AsuII TTCGAA 1 cut(s) 468
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 2 cut(s) 137, 484
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 428
BbvCI CCTCAGC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 372, 559, 795
BccI CCATC 6 cut(s) 296, 608, 613, 724, 748, 788
BceAI ACGGC 1 cut(s) 765
BcgI CGANNNNNNTGC 1 cut(s) 772
BciT130I CCWGG 1 cut(s) 122
BcnI CCSGG 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 418
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 5 cut(s) 78, 299, 368, 450, 552
BfmI CTRYAG 2 cut(s) 234, 580
BfuAI ACCTGC 1 cut(s) 155
BglII AGATCT 1 cut(s) 680
BisI GCNGC 4 cut(s) 361, 573, 781, 784
BlsI GCNGC 4 cut(s) 362, 574, 782, 785
Bme1390I CCNGG 2 cut(s) 122, 433
Bme18I GGWCC 2 cut(s) 137, 484
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 137, 265, 484
BmiI GGNNCC 2 cut(s) 430, 486
BmrFI CCNGG 2 cut(s) 122, 433
BmsI GCATC 2 cut(s) 528, 613
Bpu10I CCTNAGC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 433
Bsa29I ATCGAT 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 268, 274
BsaWI WCCGGW 1 cut(s) 738
BsaXI ACNNNNNCTCC 2 cut(s) 670, 700
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 164, 280, 348
Bse118I RCCGGY 3 cut(s) 134, 225, 738
Bse3DI GCAATG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 122
BseCI ATCGAT 1 cut(s) 329
BseDI CCNNGG 2 cut(s) 268, 274
BseGI GGATG 4 cut(s) 226, 624, 628, 775
BseLI CCNNNNNNNGG 4 cut(s) 34, 164, 280, 348
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 162, 516
BseXI GCAGC 3 cut(s) 372, 559, 795
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 4 cut(s) 50, 267, 273, 780
BshNI GGYRCC 1 cut(s) 428
BshTI ACCGGT 1 cut(s) 738
BshVI ATCGAT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 4 cut(s) 135, 226, 432, 739
BslI CCNNNNNNNGG 4 cut(s) 34, 164, 280, 348
BsmAI GTCTC 1 cut(s) 418
BsnI GGCC 4 cut(s) 50, 267, 273, 780
Bso31I GGTCTC 1 cut(s) 418
BsoBI CYCGRG 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 468
Bsp143I GATC 3 cut(s) 193, 664, 680
BspACI CCGC 2 cut(s) 401, 781
BspANI GGCC 4 cut(s) 50, 267, 273, 780
BspCNI CTCAG 2 cut(s) 161, 515
BspDI ATCGAT 1 cut(s) 329
BspLI GGNNCC 2 cut(s) 430, 486
BspMI ACCTGC 1 cut(s) 155
BspPI GGATC 1 cut(s) 672
BspT104I TTCGAA 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 316
BsrFI RCCGGY 3 cut(s) 134, 225, 738
BssAI RCCGGY 3 cut(s) 134, 225, 738
BssECI CCNNGG 2 cut(s) 268, 274
BssMI GATC 3 cut(s) 193, 664, 680
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 4 cut(s) 12, 208, 235, 262
Bst6I CTCTTC 1 cut(s) 586
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 2 cut(s) 175, 179
BstDEI CTNAG 3 cut(s) 64, 148, 502
BstF5I GGATG 4 cut(s) 226, 624, 628, 775
BstKTI GATC 3 cut(s) 196, 667, 683
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 3 cut(s) 193, 664, 680
BstMCI CGRYCG 1 cut(s) 420
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 120, 431
BstSFI CTRYAG 2 cut(s) 234, 580
BstV1I GCAGC 3 cut(s) 372, 559, 795
BstX2I RGATCY 2 cut(s) 664, 680
BstYI RGATCY 2 cut(s) 664, 680
Bsu15I ATCGAT 1 cut(s) 329
BsuRI GGCC 4 cut(s) 50, 267, 273, 780
BsuTUI ATCGAT 1 cut(s) 329
BtgZI GCGATG 1 cut(s) 767
BtsCI GGATG 4 cut(s) 226, 624, 628, 775
BtsIMutI CAGTG 1 cut(s) 781
BveI ACCTGC 1 cut(s) 155
Cac8I GCNNGC 2 cut(s) 175, 179
CaiI CAGNNNCTG 1 cut(s) 667
Cfr10I RCCGGY 3 cut(s) 134, 225, 738
Cfr13I GGNCC 3 cut(s) 137, 265, 484
ClaI ATCGAT 1 cut(s) 329
CseI GACGC 1 cut(s) 315
Csp6I GTAC 1 cut(s) 155
CspAI ACCGGT 1 cut(s) 738
CviAII CATG 1 cut(s) 317
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 3 cut(s) 64, 148, 502
DpnI GATC 3 cut(s) 195, 666, 682
DpnII GATC 3 cut(s) 193, 664, 680
EaeI YGGCCR 2 cut(s) 48, 778
Eam1104I CTCTTC 1 cut(s) 586
EarI CTCTTC 1 cut(s) 586
Eco130I CCWWGG 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 137, 484
Eco88I CYCGRG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 1 cut(s) 320
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 1 cut(s) 316
FblI GTMKAC 1 cut(s) 649
Fnu4HI GCNGC 4 cut(s) 361, 573, 781, 784
FokI GGATG 4 cut(s) 233, 631, 635, 782
Fsp4HI GCNGC 4 cut(s) 361, 573, 781, 784
FspBI CTAG 5 cut(s) 78, 299, 368, 450, 552
GluI GCNGC 4 cut(s) 361, 573, 781, 784
HaeIII GGCC 4 cut(s) 50, 267, 273, 780
HapII CCGG 4 cut(s) 135, 226, 432, 739
HgaI GACGC 1 cut(s) 315
Hin1II CATG 1 cut(s) 320
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 2 cut(s) 244, 513
HpaII CCGG 4 cut(s) 135, 226, 432, 739
HphI GGTGA 2 cut(s) 331, 737
Hpy166II GTNNAC 4 cut(s) 339, 484, 650, 736
Hpy188I TCNGA 1 cut(s) 249
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 4 cut(s) 339, 484, 650, 736
HpyCH4III ACNGT 4 cut(s) 12, 208, 235, 262
HpyCH4V TGCA 5 cut(s) 164, 173, 497, 529, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 3 cut(s) 64, 148, 502
Hsp92II CATG 1 cut(s) 320
Kzo9I GATC 3 cut(s) 193, 664, 680
Lsp1109I GCAGC 3 cut(s) 372, 559, 795
LweI GCATC 2 cut(s) 528, 613
MaeI CTAG 5 cut(s) 78, 299, 368, 450, 552
MaeIII GTNAC 2 cut(s) 214, 286
MalI GATC 3 cut(s) 195, 666, 682
MboI GATC 3 cut(s) 193, 664, 680
MboII GAAGA 1 cut(s) 603
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 2 cut(s) 664, 680
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 3 cut(s) 16, 159, 392
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 9 cut(s) 157, 180, 263, 342, 391, 403, 446, 587, 621
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 499
MscI TGGCCA 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 4 cut(s) 135, 226, 432, 739
MspR9I CCNGG 2 cut(s) 122, 433
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 170
NciI CCSGG 1 cut(s) 433
NdeII GATC 3 cut(s) 193, 664, 680
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 2 cut(s) 430, 486
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 499
NspV TTCGAA 1 cut(s) 468
PcsI WCGNNNNNNNCGW 2 cut(s) 703, 755
PfeI GAWTC 1 cut(s) 513
PflMI CCANNNNNTGG 1 cut(s) 280
PfoI TCCNGGA 1 cut(s) 120
PinAI ACCGGT 1 cut(s) 738
PkrI GCNGC 4 cut(s) 362, 574, 782, 785
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 430, 486
PspPI GGNCC 3 cut(s) 137, 265, 484
PstNI CAGNNNCTG 1 cut(s) 667
PsuI RGATCY 2 cut(s) 664, 680
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SatI GCNGC 4 cut(s) 361, 573, 781, 784
Sau3AI GATC 3 cut(s) 193, 664, 680
Sau96I GGNCC 3 cut(s) 137, 265, 484
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 122, 433
SetI ASST 9 cut(s) 108, 149, 169, 183, 191, 353, 414, 581, 612
SfaNI GCATC 2 cut(s) 528, 613
SfcI CTRYAG 2 cut(s) 234, 580
SfuI TTCGAA 1 cut(s) 468
SinI GGWCC 2 cut(s) 137, 484
SpeI ACTAGT 1 cut(s) 551
Sse9I AATT 3 cut(s) 16, 159, 392
SsiI CCGC 2 cut(s) 401, 781
SspI AATATT 1 cut(s) 520
SspMI CTAG 5 cut(s) 78, 299, 368, 450, 552
StyD4I CCNGG 2 cut(s) 120, 431
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 4 cut(s) 12, 208, 235, 262
TaqI TCGA 6 cut(s) 192, 329, 396, 468, 697, 792
TaqII GACCGA 1 cut(s) 406
TasI AATT 3 cut(s) 16, 159, 392
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 1 cut(s) 513
TscAI CASTG 1 cut(s) 781
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 3 cut(s) 360, 572, 783
Tsp45I GTSAC 1 cut(s) 214
TspGWI ACGGA 3 cut(s) 233, 560, 667
TspRI CASTG 1 cut(s) 781
Van91I CCANNNNNTGG 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 137, 484
XbaI TCTAGA 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 649
XspI CTAG 5 cut(s) 78, 299, 368, 450, 552
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.