Prupe.3G215600_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001118
Physical Location & Seq
Reverse (-)
21831316 .. 21832141
826 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G215600.1

Sequence Viewer

Length: 534 bp
ATGGCATCAAACTTCATACCAAAGCTACTATTAGTTCTAGCCTTTGCTGCTTTTTCAGCCGGAGCTAGAAGAAGTGAGCTCGAACTCAAAGAAACCCATCTGTCCTTGTTCTTCCATGACATAGCTTCTGGTCCAAATTTCACACTAATTCCAGTTGCCGGTATCGCGGGCAAGCTCTGGACCTTCGATCAGTTTGGAACAGCTTTTGTGACGGACGATCCTCTAACCGAGACCCCTGATCGAAACGCACCCATAGTTGGGAGAGGCCAAGGCATTTATGTGACGTCAGGGCTGGATGGACGTAACACCCATGTCTTGATATCCATTGTGTTCACCAACAAGAAGTACAATGGCAGCACACTGGAGATACAAGGAACCAGCAAGCAGTTCGAGAAAGTCAGAGAGGTTTCAGTGGTGTCCGGTACCGGACAGTTTCGGTTAGCCAGGGGATATGCGACGTTCGAGACGTATCTTTGGGATCCAGCAACTTTCTACTCCGTGGAGAGGTGCAACGTTACAGTGCAACACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

178

Amino Acids

19.56

Weight (kDa)

6.91

Isoelectric Point (pI)

20.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 287
Acc65I GGTACC 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 422
AccII CGCG 1 cut(s) 167
AciI CCGC 1 cut(s) 167
AclI AACGTT 1 cut(s) 513
AclWI GGATC 3 cut(s) 212, 473, 486
AcsI RAATTY 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 284
AfaI GTAC 2 cut(s) 347, 424
AfiI CCNNNNNNNGG 4 cut(s) 158, 257, 258, 504
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 6 cut(s) 25, 65, 79, 125, 175, 203
AluI AGCT 6 cut(s) 25, 65, 79, 125, 175, 203
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 224, 458
AlwI GGATC 3 cut(s) 212, 473, 486
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 47, 354
ApoI RAATTY 1 cut(s) 136
Asp718I GGTACC 1 cut(s) 422
AspS9I GGNCC 2 cut(s) 131, 180
AsuHPI GGTGA 1 cut(s) 325
AvaII GGWCC 2 cut(s) 131, 180
BamHI GGATCC 1 cut(s) 478
BanI GGYRCC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 34, 366
BccI CCATC 2 cut(s) 105, 290
BcgI CGANNNNNNTGC 2 cut(s) 370, 404
BciT130I CCWGG 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 224, 458
BfaI CTAG 3 cut(s) 38, 66, 532
BisI GCNGC 2 cut(s) 48, 355
BlsI GCNGC 2 cut(s) 49, 356
Bme1390I CCNGG 1 cut(s) 445
Bme18I GGWCC 2 cut(s) 131, 180
BmgT120I GGNCC 2 cut(s) 131, 180
BmiI GGNNCC 3 cut(s) 376, 424, 480
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 268, 444, 498
BsaWI WCCGGW 2 cut(s) 419, 425
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 257, 258, 504
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 152, 366
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 3 cut(s) 268, 444, 498
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 4 cut(s) 158, 257, 258, 504
BseNI ACTGG 2 cut(s) 152, 366
BseXI GCAGC 2 cut(s) 34, 366
Bsh1236I CGCG 1 cut(s) 167
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 4 cut(s) 60, 159, 420, 426
BslI CCNNNNNNNGG 4 cut(s) 158, 257, 258, 504
BsmAI GTCTC 2 cut(s) 224, 458
BsmBI CGTCTC 1 cut(s) 458
BsnI GGCC 1 cut(s) 267
Bso31I GGTCTC 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 4 cut(s) 187, 217, 238, 478
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 267
BspFNI CGCG 1 cut(s) 167
BspLI GGNNCC 3 cut(s) 376, 424, 480
BspPI GGATC 3 cut(s) 212, 473, 486
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 152, 366
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 3 cut(s) 268, 444, 498
BssMI GATC 4 cut(s) 187, 217, 238, 478
BssNI GRCGYC 1 cut(s) 284
BssT1I CCWWGG 1 cut(s) 268
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 432, 520
BstACI GRCGYC 1 cut(s) 284
BstC8I GCNNGC 3 cut(s) 169, 173, 383
BstDSI CCRYGG 1 cut(s) 498
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 167
BstKTI GATC 4 cut(s) 190, 220, 241, 481
BstMAI GTCTC 2 cut(s) 224, 458
BstMBI GATC 4 cut(s) 187, 217, 238, 478
BstMWI GCNNNNNNNGC 3 cut(s) 47, 56, 164
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 2 cut(s) 34, 366
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BsuRI GGCC 1 cut(s) 267
BtgI CCRYGG 1 cut(s) 498
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 3 cut(s) 359, 417, 525
Cac8I GCNNGC 3 cut(s) 169, 173, 383
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 131, 180
Csp6I GTAC 2 cut(s) 346, 423
CviAII CATG 2 cut(s) 116, 311
CviQI GTAC 2 cut(s) 346, 423
DpnI GATC 4 cut(s) 189, 219, 240, 480
DpnII GATC 4 cut(s) 187, 217, 238, 478
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 224
Eco32I GATATC 1 cut(s) 321
Eco47I GGWCC 2 cut(s) 131, 180
Eco53kI GAGCTC 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 443
EcoRV GATATC 1 cut(s) 321
EcoT14I CCWWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 268
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 2 cut(s) 119, 314
FaiI YATR 7 cut(s) 17, 117, 122, 254, 279, 312, 453
FatI CATG 2 cut(s) 115, 310
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 48, 355
FokI GGATG 1 cut(s) 308
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 2 cut(s) 48, 355
FspBI CTAG 3 cut(s) 38, 66, 532
GluI GCNGC 2 cut(s) 48, 355
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 1 cut(s) 267
HapII CCGG 4 cut(s) 60, 159, 420, 426
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 2 cut(s) 119, 314
HpaII CCGG 4 cut(s) 60, 159, 420, 426
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 1 cut(s) 333
Hpy188I TCNGA 1 cut(s) 401
Hpy188III TCNNGA 4 cut(s) 178, 316, 391, 463
Hpy8I GTNNAC 1 cut(s) 333
Hpy99I CGWCG 1 cut(s) 460
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 2 cut(s) 432, 520
HpyCH4IV ACGT 5 cut(s) 284, 301, 458, 467, 513
HpyCH4V TGCA 2 cut(s) 510, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 56, 164
HpySE526I ACGT 5 cut(s) 284, 301, 458, 467, 513
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 2 cut(s) 119, 314
KpnI GGTACC 1 cut(s) 426
Kzo9I GATC 4 cut(s) 187, 217, 238, 478
LmnI GCTCC 1 cut(s) 62
Lsp1109I GCAGC 2 cut(s) 34, 366
LweI GCATC 1 cut(s) 14
MaeI CTAG 3 cut(s) 38, 66, 532
MaeII ACGT 5 cut(s) 284, 301, 458, 467, 513
MaeIII GTNAC 4 cut(s) 208, 280, 302, 514
MalI GATC 4 cut(s) 189, 219, 240, 480
MboI GATC 4 cut(s) 187, 217, 238, 478
MboII GAAGA 2 cut(s) 81, 103
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 2 cut(s) 136, 147
MnlI CCTC 4 cut(s) 231, 257, 397, 498
MslI CAYNNNNRTG 1 cut(s) 278
MspI CCGG 4 cut(s) 60, 159, 420, 426
MspR9I CCNGG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 445
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 3 cut(s) 47, 56, 164
NdeII GATC 4 cut(s) 187, 217, 238, 478
NlaIII CATG 2 cut(s) 119, 314
NlaIV GGNNCC 3 cut(s) 376, 424, 480
NmuCI GTSAC 2 cut(s) 208, 280
PcsI WCGNNNNNNNCGW 1 cut(s) 464
PkrI GCNGC 2 cut(s) 49, 356
Psp124BI GAGCTC 1 cut(s) 81
Psp1406I AACGTT 1 cut(s) 513
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 3 cut(s) 376, 424, 480
PspPI GGNCC 2 cut(s) 131, 180
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 2 cut(s) 347, 424
RsaNI GTAC 2 cut(s) 346, 423
RseI CAYNNNNRTG 1 cut(s) 278
SacI GAGCTC 1 cut(s) 81
SatI GCNGC 2 cut(s) 48, 355
Sau3AI GATC 4 cut(s) 187, 217, 238, 478
Sau96I GGNCC 2 cut(s) 131, 180
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 2 cut(s) 131, 180
SmiMI CAYNNNNRTG 1 cut(s) 278
Sse9I AATT 2 cut(s) 136, 147
SsiI CCGC 1 cut(s) 167
SspMI CTAG 3 cut(s) 38, 66, 532
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 443
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 2 cut(s) 432, 520
TaiI ACGT 5 cut(s) 287, 304, 461, 470, 516
TaqI TCGA 5 cut(s) 81, 186, 241, 390, 462
TasI AATT 2 cut(s) 136, 147
TatI WGTACW 1 cut(s) 345
TscAI CASTG 3 cut(s) 366, 417, 525
TseFI GTSAC 2 cut(s) 208, 280
TseI GCWGC 2 cut(s) 47, 354
Tsp45I GTSAC 2 cut(s) 208, 280
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 2 cut(s) 227, 487
TspRI CASTG 3 cut(s) 366, 417, 525
VpaK11BI GGWCC 2 cut(s) 131, 180
XapI RAATTY 1 cut(s) 136
XspI CTAG 3 cut(s) 38, 66, 532
ZraI GACGTC 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.