Rh2DG557300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001118
Physical Location & Seq
Reverse (-)
78684973 .. 78685717
745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG557300.1

Sequence Viewer

Length: 468 bp
ATGGCCAGTACTCGTGACTTCAAACAAACCCAACTGTCCATGTACTTACAAGACTTCACCGCCGGTCCAAATCTCACGGGATTGCCAATTATAGGTATTGCCGGCAAGCTTTGGACCTTTGCTCAATTTGGGACAGTTTACGTCAACGACGACCCCATAACCGAAGGCCCAAGCCCTACATCGGCAGTAGTTGGCCGAGCACAGGCTCTTTCTGTAACATCAGCACTAGACGGACAAAACGCCTTGGTCTTTTTGTCGATTGCGTTCACAAACAAGAAGTACAATGGCAGCACTATTGAGATACAAGGAAGCTATAAGCAATTTGAGCCGAGCACAGAGGTGGCGGTGGTTTCAGGTACTGGTAAATTTCGGTTTGCTAGGGGATATGCTACGTTCGAAACTTATTTCGTGGACCAACCAAATAGCTACTCTGTTATACGCTGCAATATTACAGTGCAGCACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

155

Amino Acids

16.92

Weight (kDa)

6.55

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 10 - 152 3.1e-43 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 60, 344
AcoI YGGCCR 2 cut(s) 3, 193
AcsI RAATTY 1 cut(s) 365
AfaI GTAC 4 cut(s) 10, 44, 281, 358
AfiI CCNNNNNNNGG 3 cut(s) 92, 181, 202
AgsI TTSAA 1 cut(s) 22
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 3 cut(s) 109, 312, 426
AluI AGCT 3 cut(s) 109, 312, 426
Alw21I GWGCWC 2 cut(s) 202, 335
AlwNI CAGNNNCTG 1 cut(s) 359
AoxI GGCC 3 cut(s) 3, 166, 193
ApeKI GCWGC 3 cut(s) 288, 441, 457
ApoI RAATTY 1 cut(s) 365
AspS9I GGNCC 4 cut(s) 65, 114, 167, 412
AsuHPI GGTGA 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 396
AvaII GGWCC 3 cut(s) 65, 114, 412
BalI TGGCCA 1 cut(s) 5
BauI CACGAG 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 202, 335
BbvI GCAGC 2 cut(s) 300, 428
BfaI CTAG 2 cut(s) 227, 378
BisI GCNGC 3 cut(s) 289, 442, 458
BlsI GCNGC 3 cut(s) 290, 443, 459
BmcAI AGTACT 1 cut(s) 10
Bme18I GGWCC 3 cut(s) 65, 114, 412
BmgT120I GGNCC 4 cut(s) 65, 114, 167, 412
Bpu14I TTCGAA 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 181, 202
Bse118I RCCGGY 2 cut(s) 62, 101
Bse1I ACTGG 2 cut(s) 6, 364
BseDI CCNNGG 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 92, 181, 202
BseNI ACTGG 2 cut(s) 6, 364
BseXI GCAGC 2 cut(s) 300, 428
BshFI GGCC 3 cut(s) 5, 168, 195
BsiHKAI GWGCWC 2 cut(s) 202, 335
BsiSI CCGG 2 cut(s) 63, 102
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 3 cut(s) 92, 181, 202
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 3 cut(s) 5, 168, 195
Bsp119I TTCGAA 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 202, 335
BspACI CCGC 2 cut(s) 60, 344
BspANI GGCC 3 cut(s) 5, 168, 195
BspT104I TTCGAA 1 cut(s) 396
BsrFI RCCGGY 2 cut(s) 62, 101
BsrI ACTGG 2 cut(s) 6, 364
BssAI RCCGGY 2 cut(s) 62, 101
BssECI CCNNGG 1 cut(s) 243
BssSI CACGAG 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 243
Bst2BI CACGAG 1 cut(s) 12
Bst4CI ACNGT 3 cut(s) 36, 136, 454
BstBI TTCGAA 1 cut(s) 396
BstC8I GCNNGC 2 cut(s) 103, 107
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstV1I GCAGC 2 cut(s) 300, 428
BsuRI GGCC 3 cut(s) 5, 168, 195
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 2 cut(s) 103, 107
CaiI CAGNNNCTG 1 cut(s) 359
Cfr10I RCCGGY 2 cut(s) 62, 101
Cfr13I GGNCC 4 cut(s) 65, 114, 167, 412
Csp6I GTAC 4 cut(s) 9, 43, 280, 357
CviAII CATG 1 cut(s) 40
CviJI RGCY 9 cut(s) 5, 109, 168, 174, 195, 206, 312, 328, 426
CviKI_1 RGCY 9 cut(s) 5, 109, 168, 174, 195, 206, 312, 328, 426
CviQI GTAC 4 cut(s) 9, 43, 280, 357
EaeI YGGCCR 2 cut(s) 3, 193
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 3 cut(s) 65, 114, 412
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 1 cut(s) 43
FaiI YATR 6 cut(s) 41, 92, 158, 315, 387, 437
FaqI GGGAC 1 cut(s) 145
FatI CATG 1 cut(s) 39
Fnu4HI GCNGC 3 cut(s) 289, 442, 458
Fsp4HI GCNGC 3 cut(s) 289, 442, 458
FspBI CTAG 2 cut(s) 227, 378
GluI GCNGC 3 cut(s) 289, 442, 458
HaeIII GGCC 3 cut(s) 5, 168, 195
HapII CCGG 2 cut(s) 63, 102
Hin1II CATG 1 cut(s) 43
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 107
HpaII CCGG 2 cut(s) 63, 102
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 4 cut(s) 139, 145, 267, 412
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 4 cut(s) 139, 145, 267, 412
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 3 cut(s) 36, 136, 454
HpyCH4IV ACGT 2 cut(s) 141, 392
HpyCH4V TGCA 2 cut(s) 444, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpySE526I ACGT 2 cut(s) 141, 392
Hsp92II CATG 1 cut(s) 43
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
LpnPI CCDG 6 cut(s) 19, 76, 115, 188, 339, 345
Lsp1109I GCAGC 2 cut(s) 300, 428
MaeI CTAG 2 cut(s) 227, 378
MaeII ACGT 2 cut(s) 141, 392
MaeIII GTNAC 2 cut(s) 14, 214
MhlI GDGCHC 2 cut(s) 202, 335
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 87, 125, 320, 365
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 1 cut(s) 331
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 101
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 63, 102
MwoI GCNNNNNNNGC 1 cut(s) 325
NaeI GCCGGC 1 cut(s) 103
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 43
NmeAIII GCCGAG 2 cut(s) 221, 354
NmuCI GTSAC 1 cut(s) 14
NspV TTCGAA 1 cut(s) 396
OliI CACNNNNGTG 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 290, 443, 459
PspPI GGNCC 4 cut(s) 65, 114, 167, 412
PstNI CAGNNNCTG 1 cut(s) 359
RsaI GTAC 4 cut(s) 10, 44, 281, 358
RsaNI GTAC 4 cut(s) 9, 43, 280, 357
RseI CAYNNNNRTG 1 cut(s) 338
SatI GCNGC 3 cut(s) 289, 442, 458
Sau96I GGNCC 4 cut(s) 65, 114, 167, 412
ScaI AGTACT 1 cut(s) 10
SduI GDGCHC 2 cut(s) 202, 335
SetI ASST 9 cut(s) 97, 111, 119, 144, 314, 342, 358, 395, 428
SfuI TTCGAA 1 cut(s) 396
SinI GGWCC 3 cut(s) 65, 114, 412
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 4 cut(s) 87, 125, 320, 365
SsiI CCGC 2 cut(s) 60, 344
SspI AATATT 1 cut(s) 448
SspMI CTAG 2 cut(s) 227, 378
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 3 cut(s) 36, 136, 454
TaiI ACGT 2 cut(s) 144, 395
TaqI TCGA 2 cut(s) 257, 396
TasI AATT 4 cut(s) 87, 125, 320, 365
TatI WGTACW 3 cut(s) 8, 42, 279
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 3 cut(s) 288, 441, 457
Tsp45I GTSAC 1 cut(s) 14
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 1 cut(s) 459
VpaK11BI GGWCC 3 cut(s) 65, 114, 412
XapI RAATTY 1 cut(s) 365
XspI CTAG 2 cut(s) 227, 378
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.