FvH4_6g42930

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001118
Physical Location & Seq
Reverse (-)
33324744 .. 33325420
677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42930.t1

Sequence Viewer

Length: 435 bp
ATGGTCTTGTACTTCCAAGACTTCTCTGGAGGCCCGAATGCCACTAGCATTCCAATTGCAGGGATTGCCGGAAAGCTTTGGACCTTCACTCAGTTCGGAACAGTTTACGTCATTGACGACACCATGACCCAAGGTCCAAGCCCAAAGTCGGCCGTAGTTGGCCGCGCACAAGGCGTGACTGTGGCGGCGGCACTAGACGGGAGCAATGCCCTCGTCTTGGTATCGCTTGTGTTCACAAACAGGGAGTACAATGGCAGCACTATAGAGATACAAGGAAACAGTAAGCAGTTCCAGCCAGTTAGAGAGGTTGCGGTGGTATCAGGTACCGGAAAATTCAGGTTTGCTAGGGGATATGCTACCTTTGAAACGTACTTCGTGGATCTTCCGAGTGCCTACTCCATATTACGGTGCAATGTTACAGTGCAGCATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

145

Amino Acids

15.53

Weight (kDa)

7.84

Isoelectric Point (pI)

22.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 141 4.8e-47 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 323
AccII CGCG 1 cut(s) 165
AciI CCGC 4 cut(s) 163, 185, 188, 311
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 2 cut(s) 150, 160
AcsI RAATTY 1 cut(s) 332
AfaI GTAC 4 cut(s) 11, 248, 325, 371
AfiI CCNNNNNNNGG 4 cut(s) 59, 148, 217, 405
AgsI TTSAA 1 cut(s) 365
AhdI GACNNNNNGTC 1 cut(s) 132
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
AlwI GGATC 1 cut(s) 387
AoxI GGCC 3 cut(s) 31, 150, 160
ApeKI GCWGC 2 cut(s) 255, 424
ApoI RAATTY 1 cut(s) 332
Asp718I GGTACC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 3 cut(s) 32, 81, 134
AvaII GGWCC 2 cut(s) 81, 134
BanI GGYRCC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 267
BceAI ACGGC 1 cut(s) 137
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BfaI CTAG 4 cut(s) 45, 194, 345, 433
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 5 cut(s) 163, 186, 189, 256, 425
BlsI GCNGC 5 cut(s) 164, 187, 190, 257, 426
Bme18I GGWCC 2 cut(s) 81, 134
BmeRI GACNNNNNGTC 1 cut(s) 132
BmgT120I GGNCC 3 cut(s) 32, 81, 134
BmiI GGNNCC 1 cut(s) 325
BpmI CTGGAG 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 1 cut(s) 326
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 148, 217, 405
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 2 cut(s) 211, 418
BseDI CCNNGG 1 cut(s) 130
BseLI CCNNNNNNNGG 4 cut(s) 59, 148, 217, 405
BseMI GCAATG 2 cut(s) 211, 418
BseMII CTCAG 1 cut(s) 104
BseNI ACTGG 1 cut(s) 296
BseX3I CGGCCG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 3 cut(s) 33, 152, 162
BshNI GGYRCC 1 cut(s) 323
BsiEI CGRYCG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 69, 327
BslI CCNNNNNNNGG 4 cut(s) 59, 148, 217, 405
BsmI GAATGC 2 cut(s) 43, 48
BsnI GGCC 3 cut(s) 33, 152, 162
Bsp143I GATC 1 cut(s) 379
BspACI CCGC 4 cut(s) 163, 185, 188, 311
BspANI GGCC 3 cut(s) 33, 152, 162
BspCNI CTCAG 1 cut(s) 103
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 325
BspPI GGATC 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 323
BsrDI GCAATG 2 cut(s) 211, 418
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 130
Bst4CI ACNGT 5 cut(s) 103, 181, 281, 408, 421
BstAPI GCANNNNNTGC 1 cut(s) 65
BstDEI CTNAG 1 cut(s) 90
BstFNI CGCG 1 cut(s) 165
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 1 cut(s) 382
BstMBI GATC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 3 cut(s) 65, 171, 292
BstSFI CTRYAG 1 cut(s) 261
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 150
BsuRI GGCC 3 cut(s) 33, 152, 162
BtsIMutI CAGTG 1 cut(s) 426
CfoI GCGC 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 32, 81, 134
Csp6I GTAC 4 cut(s) 10, 247, 324, 370
CviAII CATG 1 cut(s) 124
CviJI RGCY 6 cut(s) 33, 76, 141, 152, 162, 295
CviKI_1 RGCY 6 cut(s) 33, 76, 141, 152, 162, 295
CviQI GTAC 4 cut(s) 10, 247, 324, 370
DdeI CTNAG 1 cut(s) 90
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 132
EaeI YGGCCR 2 cut(s) 150, 160
EagI CGGCCG 1 cut(s) 150
Eam1105I GACNNNNNGTC 1 cut(s) 132
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 130
Eco47I GGWCC 2 cut(s) 81, 134
Eco52I CGGCCG 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 127
FaiI YATR 4 cut(s) 125, 263, 354, 401
FatI CATG 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 163, 186, 189, 256, 425
Fsp4HI GCNGC 5 cut(s) 163, 186, 189, 256, 425
FspBI CTAG 4 cut(s) 45, 194, 345, 433
GlaI GCGC 1 cut(s) 166
GluI GCNGC 5 cut(s) 163, 186, 189, 256, 425
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 3 cut(s) 33, 152, 162
HapII CCGG 2 cut(s) 69, 327
HhaI GCGC 1 cut(s) 167
Hin1II CATG 1 cut(s) 127
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 74
HpaII CCGG 2 cut(s) 69, 327
Hpy166II GTNNAC 2 cut(s) 106, 234
Hpy188I TCNGA 2 cut(s) 98, 387
Hpy188III TCNNGA 1 cut(s) 27
Hpy8I GTNNAC 2 cut(s) 106, 234
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 5 cut(s) 103, 181, 281, 408, 421
HpyCH4IV ACGT 2 cut(s) 108, 368
HpyCH4V TGCA 3 cut(s) 59, 411, 424
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 171, 292
HpyF3I CTNAG 1 cut(s) 90
HpySE526I ACGT 2 cut(s) 108, 368
Hsp92II CATG 1 cut(s) 127
HspAI GCGC 1 cut(s) 165
KpnI GGTACC 1 cut(s) 327
Kzo9I GATC 1 cut(s) 379
LmnI GCTCC 1 cut(s) 201
LpnPI CCDG 9 cut(s) 12, 45, 82, 226, 305, 306, 309, 322, 340
Lsp1109I GCAGC 1 cut(s) 267
MaeI CTAG 4 cut(s) 45, 194, 345, 433
MaeII ACGT 2 cut(s) 108, 368
MaeIII GTNAC 2 cut(s) 175, 415
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 1 cut(s) 374
MfeI CAATTG 1 cut(s) 54
MflI RGATCY 1 cut(s) 379
MluCI AATT 2 cut(s) 54, 332
MnlI CCTC 3 cut(s) 23, 221, 298
MspI CCGG 2 cut(s) 69, 327
MunI CAATTG 1 cut(s) 54
Mva1269I GAATGC 2 cut(s) 43, 48
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 3 cut(s) 65, 171, 292
NdeII GATC 1 cut(s) 379
NlaIII CATG 1 cut(s) 127
NlaIV GGNNCC 1 cut(s) 325
NmuCI GTSAC 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 2 cut(s) 43, 48
PkrI GCNGC 5 cut(s) 164, 187, 190, 257, 426
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 3 cut(s) 32, 81, 134
PsuI RGATCY 1 cut(s) 379
RsaI GTAC 4 cut(s) 11, 248, 325, 371
RsaNI GTAC 4 cut(s) 10, 247, 324, 370
SatI GCNGC 5 cut(s) 163, 186, 189, 256, 425
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 3 cut(s) 32, 81, 134
SetI ASST 9 cut(s) 78, 86, 111, 136, 309, 325, 341, 362, 371
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 81, 134
Sse9I AATT 2 cut(s) 54, 332
SsiI CCGC 4 cut(s) 163, 185, 188, 311
SspMI CTAG 4 cut(s) 45, 194, 345, 433
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 5 cut(s) 103, 181, 281, 408, 421
TaiI ACGT 2 cut(s) 111, 371
TasI AATT 2 cut(s) 54, 332
TatI WGTACW 2 cut(s) 9, 246
TauI GCSGC 3 cut(s) 165, 188, 191
TscAI CASTG 1 cut(s) 426
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 2 cut(s) 255, 424
Tsp45I GTSAC 1 cut(s) 175
TspRI CASTG 1 cut(s) 426
VpaK11BI GGWCC 2 cut(s) 81, 134
XapI RAATTY 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XspI CTAG 4 cut(s) 45, 194, 345, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.