Rw2G044240

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001118
Physical Location & Seq
Reverse (-)
70918468 .. 70922646
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044240.1

Sequence Viewer

Length: 555 bp
ATGGCATTAGCCATATCCCCACTGTTCATTTTGGTTCTGAGTACTTTTTTAGCCATGGCCAGTACTCGTGACTTCAAACAAACCCAACTGTCCATGTACTTACACGACTTCACCGCCGGTCCAAATCTCACGGGATTACCAATTATAGGTATTGCCGGCAAGCTTTGGACCTTTGCTCAATTTGGGACAGTTTACTTTTGGACCTTTGATCAATTTGGAACAGTTTATGTCACCGACGACCCCATAACCGAAGGCCCAAGCCCTAAATCTGCACCAGTTGGCCGAGGACAGGCTTTTATTGTAACATCAGCACTAGATGGACGTAACGCCCTAGTTTTTCTCTCGATTGTGTTCACAAACAAGAAGTACAACGGTAGCACTATTGAGATACAAGGAAACAGTATGCAATTTGAGCCGGTAACAGAGGTGGCCGTGGTTTCAGGTACTGGTAAATTTCGGTTTGCTAGGGGATATGCTACGCTCGAAACTTATTTCGTGGACCAACCAAGGGGCTACTCTGTTTTACGCTGCAATATTACAGTGCAACACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

184

Amino Acids

20.28

Weight (kDa)

7.83

Isoelectric Point (pI)

26.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 28 - 181 2.3e-41 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 114
AcoI YGGCCR 3 cut(s) 57, 280, 429
AcsI RAATTY 1 cut(s) 452
AfaI GTAC 5 cut(s) 43, 64, 98, 368, 445
AfiI CCNNNNNNNGG 3 cut(s) 146, 289, 508
AgsI TTSAA 1 cut(s) 76
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
AlwNI CAGNNNCTG 1 cut(s) 446
AoxI GGCC 4 cut(s) 57, 253, 280, 429
ApeKI GCWGC 1 cut(s) 528
ApoI RAATTY 1 cut(s) 452
AspS9I GGNCC 5 cut(s) 119, 168, 201, 254, 499
AsuHPI GGTGA 2 cut(s) 103, 223
AvaII GGWCC 4 cut(s) 119, 168, 201, 499
BalI TGGCCA 1 cut(s) 59
BauI CACGAG 1 cut(s) 66
BbvI GCAGC 1 cut(s) 515
BccI CCATC 1 cut(s) 311
BceAI ACGGC 1 cut(s) 416
BclI TGATCA 1 cut(s) 208
BfaI CTAG 3 cut(s) 314, 332, 465
BisI GCNGC 1 cut(s) 529
BlsI GCNGC 1 cut(s) 530
BmcAI AGTACT 2 cut(s) 43, 64
Bme18I GGWCC 4 cut(s) 119, 168, 201, 499
BmgT120I GGNCC 5 cut(s) 119, 168, 201, 254, 499
BsaJI CCNNGG 4 cut(s) 54, 283, 432, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 289, 508
Bse118I RCCGGY 3 cut(s) 116, 155, 415
Bse1I ACTGG 3 cut(s) 60, 275, 451
BseDI CCNNGG 4 cut(s) 54, 283, 432, 506
BseLI CCNNNNNNNGG 3 cut(s) 146, 289, 508
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 3 cut(s) 60, 275, 451
BseXI GCAGC 1 cut(s) 515
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 4 cut(s) 59, 255, 282, 431
BsiSI CCGG 3 cut(s) 117, 156, 416
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 3 cut(s) 146, 289, 508
BsmFI GGGAC 1 cut(s) 199
BsnI GGCC 4 cut(s) 59, 255, 282, 431
Bsp143I GATC 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 1 cut(s) 114
BspANI GGCC 4 cut(s) 59, 255, 282, 431
BspCNI CTCAG 1 cut(s) 30
BsrFI RCCGGY 3 cut(s) 116, 155, 415
BsrI ACTGG 3 cut(s) 60, 275, 451
BssAI RCCGGY 3 cut(s) 116, 155, 415
BssECI CCNNGG 4 cut(s) 54, 283, 432, 506
BssMI GATC 1 cut(s) 208
BssSI CACGAG 1 cut(s) 66
BssT1I CCWWGG 2 cut(s) 54, 506
Bst2BI CACGAG 1 cut(s) 66
Bst4CI ACNGT 7 cut(s) 24, 90, 190, 223, 374, 401, 541
BstC8I GCNNGC 2 cut(s) 157, 161
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 2 cut(s) 54, 432
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 412
BstV1I GCAGC 1 cut(s) 515
BsuRI GGCC 4 cut(s) 59, 255, 282, 431
BtgI CCRYGG 2 cut(s) 54, 432
BtsIMutI CAGTG 2 cut(s) 20, 546
Cac8I GCNNGC 2 cut(s) 157, 161
CaiI CAGNNNCTG 1 cut(s) 446
Cfr10I RCCGGY 3 cut(s) 116, 155, 415
Cfr13I GGNCC 5 cut(s) 119, 168, 201, 254, 499
Csp6I GTAC 5 cut(s) 42, 63, 97, 367, 444
CviAII CATG 2 cut(s) 55, 94
CviQI GTAC 5 cut(s) 42, 63, 97, 367, 444
DdeI CTNAG 1 cut(s) 38
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
EaeI YGGCCR 3 cut(s) 57, 280, 429
Eco130I CCWWGG 2 cut(s) 54, 506
Eco47I GGWCC 4 cut(s) 119, 168, 201, 499
EcoT14I CCWWGG 2 cut(s) 54, 506
ErhI CCWWGG 2 cut(s) 54, 506
FaeI CATG 2 cut(s) 58, 97
FaiI YATR 8 cut(s) 14, 56, 95, 146, 228, 245, 404, 474
FaqI GGGAC 1 cut(s) 199
FatI CATG 2 cut(s) 54, 93
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 529
Fsp4HI GCNGC 1 cut(s) 529
FspBI CTAG 3 cut(s) 314, 332, 465
GluI GCNGC 1 cut(s) 529
HaeIII GGCC 4 cut(s) 59, 255, 282, 431
HapII CCGG 3 cut(s) 117, 156, 416
Hin1II CATG 2 cut(s) 58, 97
HindIII AAGCTT 1 cut(s) 161
HpaII CCGG 3 cut(s) 117, 156, 416
HphI GGTGA 2 cut(s) 103, 223
Hpy166II GTNNAC 3 cut(s) 193, 354, 499
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 2 cut(s) 68, 343
Hpy8I GTNNAC 3 cut(s) 193, 354, 499
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 7 cut(s) 24, 90, 190, 223, 374, 401, 541
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 4 cut(s) 272, 406, 531, 544
HpyF10VI GCNNNNNNNGC 1 cut(s) 412
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 322
Hsp92II CATG 2 cut(s) 58, 97
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 1 cut(s) 208
LpnPI CCDG 8 cut(s) 73, 130, 169, 275, 288, 426, 429, 432
Lsp1109I GCAGC 1 cut(s) 515
MaeI CTAG 3 cut(s) 314, 332, 465
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 5 cut(s) 68, 229, 301, 323, 418
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 5 cut(s) 141, 179, 212, 407, 452
MluNI TGGCCA 1 cut(s) 59
MnlI CCTC 2 cut(s) 278, 418
Mox20I TGGCCA 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 3 cut(s) 117, 156, 416
MwoI GCNNNNNNNGC 1 cut(s) 412
NaeI GCCGGC 1 cut(s) 157
NcoI CCATGG 1 cut(s) 54
NdeII GATC 1 cut(s) 208
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 2 cut(s) 58, 97
NmeAIII GCCGAG 1 cut(s) 308
NmuCI GTSAC 2 cut(s) 68, 229
PdiI GCCGGC 1 cut(s) 157
PkrI GCNGC 1 cut(s) 530
PspPI GGNCC 5 cut(s) 119, 168, 201, 254, 499
PstNI CAGNNNCTG 1 cut(s) 446
RsaI GTAC 5 cut(s) 43, 64, 98, 368, 445
RsaNI GTAC 5 cut(s) 42, 63, 97, 367, 444
SatI GCNGC 1 cut(s) 529
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 5 cut(s) 119, 168, 201, 254, 499
ScaI AGTACT 2 cut(s) 43, 64
SetI ASST 7 cut(s) 151, 165, 173, 206, 325, 429, 445
SinI GGWCC 4 cut(s) 119, 168, 201, 499
Sse9I AATT 5 cut(s) 141, 179, 212, 407, 452
SsiI CCGC 1 cut(s) 114
SspI AATATT 1 cut(s) 535
SspMI CTAG 3 cut(s) 314, 332, 465
StyI CCWWGG 2 cut(s) 54, 506
TaaI ACNGT 7 cut(s) 24, 90, 190, 223, 374, 401, 541
TaiI ACGT 1 cut(s) 325
TaqI TCGA 2 cut(s) 344, 483
TasI AATT 5 cut(s) 141, 179, 212, 407, 452
TatI WGTACW 4 cut(s) 41, 62, 96, 366
TscAI CASTG 2 cut(s) 27, 546
TseFI GTSAC 2 cut(s) 68, 229
TseI GCWGC 1 cut(s) 528
Tsp45I GTSAC 2 cut(s) 68, 229
TspDTI ATGAA 1 cut(s) 16
TspRI CASTG 2 cut(s) 27, 546
VpaK11BI GGWCC 4 cut(s) 119, 168, 201, 499
XapI RAATTY 1 cut(s) 452
XspI CTAG 3 cut(s) 314, 332, 465
ZrmI AGTACT 2 cut(s) 43, 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.