pycom09g03340

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0001118
Physical Location & Seq
Forward (+)
2478830 .. 2479357
528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g03340.1

Sequence Viewer

Length: 528 bp
ATGGCATTGAACTTCATAGCAAAGTTGCTATTAGTTCTAACCTTTGCCGCTTTTTCGGCCGGTGCTACAAGAAGTCAGCTCAAAGAAACCAAACTGACTGTGTTCTTCCATGACTTCGTTGCCGGTCCGAATACCACAGTAATCCCAGTTGCAGGTATTGCCGGCAAACTTTGGACCTTCACTCAGTTCGGAACAGTTTACGTGACTGATGATCCGTTAACCGCAACACCGGATCTAAACTCAGCCGTAGTCGGGCGAGGCCAAGGCGTGTATGTGACATCCGGGCTGGACGGACTCAACACTCATGTGTTGATATCGATTGTGTTCACAAACAAGAAGTACAATGGCAGCACATTAGAGATACAAGGAACCAGTAAGCAGTTCGAGGCGGTTAGGGAGGTTTCGGTGGTCTCCGGTACCGGAAAGTTTCGATTTGCGAGAGGATACGCTACGTTCGAAACGTTGCTTGTGGATGCTGCAACTTTCTATTCGGTTGAGAGATGCAATGTTACAGTGGAACATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

176

Amino Acids

18.93

Weight (kDa)

7.85

Isoelectric Point (pI)

10.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 30 - 172 1.1e-45 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 143
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 416
AciI CCGC 3 cut(s) 48, 222, 389
AclI AACGTT 1 cut(s) 461
AclWI GGATC 2 cut(s) 206, 240
AcoI YGGCCR 1 cut(s) 57
AfaI GTAC 2 cut(s) 341, 418
AfiI CCNNNNNNNGG 2 cut(s) 152, 252
AgsI TTSAA 1 cut(s) 10
AleI CACNNNNGTG 1 cut(s) 305
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw26I GTCTC 1 cut(s) 415
AlwI GGATC 2 cut(s) 206, 240
AoxI GGCC 2 cut(s) 57, 259
ApeKI GCWGC 2 cut(s) 348, 476
Asp718I GGTACC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 125, 174
AsuC2I CCSGG 1 cut(s) 283
AsuII TTCGAA 1 cut(s) 456
AvaII GGWCC 2 cut(s) 125, 174
BanI GGYRCC 1 cut(s) 416
BbvI GCAGC 2 cut(s) 360, 463
BceAI ACGGC 1 cut(s) 230
BciVI GTATCC 1 cut(s) 437
BcnI CCSGG 1 cut(s) 283
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 1 cut(s) 526
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 1 cut(s) 437
BisI GCNGC 3 cut(s) 48, 349, 477
BlsI GCNGC 3 cut(s) 49, 350, 478
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 2 cut(s) 125, 174
BmgT120I GGNCC 2 cut(s) 125, 174
BmiI GGNNCC 2 cut(s) 370, 418
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 463, 491
BmuI ACTGGG 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 283
Bsa29I ATCGAT 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 202
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 1 cut(s) 262
BsaWI WCCGGW 3 cut(s) 229, 413, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 252
Bse118I RCCGGY 3 cut(s) 59, 122, 161
Bse1I ACTGG 2 cut(s) 146, 372
Bse3DI GCAATG 1 cut(s) 511
BseCI ATCGAT 1 cut(s) 317
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 2 cut(s) 278, 478
BseLI CCNNNNNNNGG 2 cut(s) 152, 252
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 2 cut(s) 197, 255
BseNI ACTGG 2 cut(s) 146, 372
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 360, 463
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 261
BshNI GGYRCC 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 317
BsiEI CGRYCG 1 cut(s) 60
BsiSI CCGG 7 cut(s) 60, 123, 162, 230, 282, 414, 420
BslI CCNNNNNNNGG 2 cut(s) 152, 252
BsmAI GTCTC 1 cut(s) 415
BsnI GGCC 2 cut(s) 59, 261
Bso31I GGTCTC 1 cut(s) 415
Bsp119I TTCGAA 1 cut(s) 456
Bsp143I GATC 2 cut(s) 211, 232
BspACI CCGC 3 cut(s) 48, 222, 389
BspANI GGCC 2 cut(s) 59, 261
BspCNI CTCAG 2 cut(s) 196, 254
BspDI ATCGAT 1 cut(s) 317
BspLI GGNNCC 2 cut(s) 370, 418
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 2 cut(s) 206, 240
BspT104I TTCGAA 1 cut(s) 456
BspT107I GGYRCC 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 511
BsrFI RCCGGY 3 cut(s) 59, 122, 161
BsrI ACTGG 2 cut(s) 146, 372
BssAI RCCGGY 3 cut(s) 59, 122, 161
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 2 cut(s) 211, 232
BssT1I CCWWGG 1 cut(s) 262
Bst4CI ACNGT 4 cut(s) 100, 139, 196, 514
BstAPI GCANNNNNTGC 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 202
BstBI TTCGAA 1 cut(s) 456
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 183, 241
BstF5I GGATG 2 cut(s) 278, 478
BstKTI GATC 2 cut(s) 214, 235
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 2 cut(s) 211, 232
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 2 cut(s) 56, 158
BstSCI CCNGG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 360, 463
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BstZI CGGCCG 1 cut(s) 57
Bsu15I ATCGAT 1 cut(s) 317
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 2 cut(s) 59, 261
BsuTUI ATCGAT 1 cut(s) 317
BtsCI GGATG 2 cut(s) 278, 478
BtsIMutI CAGTG 1 cut(s) 519
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 163
Cfr10I RCCGGY 3 cut(s) 59, 122, 161
Cfr13I GGNCC 2 cut(s) 125, 174
ClaI ATCGAT 1 cut(s) 317
CpoI CGGWCCG 1 cut(s) 125
Csp6I GTAC 2 cut(s) 340, 417
CspI CGGWCCG 1 cut(s) 125
CviAII CATG 2 cut(s) 110, 305
CviJI RGCY 5 cut(s) 59, 79, 245, 261, 286
CviKI_1 RGCY 5 cut(s) 59, 79, 245, 261, 286
CviQI GTAC 2 cut(s) 340, 417
DdeI CTNAG 2 cut(s) 183, 241
DpnI GATC 2 cut(s) 213, 234
DpnII GATC 2 cut(s) 211, 232
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 262
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 125, 174
Eco52I CGGCCG 1 cut(s) 57
EcoRV GATATC 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 2 cut(s) 113, 308
FaiI YATR 4 cut(s) 17, 111, 273, 306
FatI CATG 2 cut(s) 109, 304
Fnu4HI GCNGC 3 cut(s) 48, 349, 477
FokI GGATG 2 cut(s) 265, 485
Fsp4HI GCNGC 3 cut(s) 48, 349, 477
FspBI CTAG 1 cut(s) 526
GluI GCNGC 3 cut(s) 48, 349, 477
HaeIII GGCC 2 cut(s) 59, 261
HapII CCGG 7 cut(s) 60, 123, 162, 230, 282, 414, 420
Hin1II CATG 2 cut(s) 113, 308
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HinfI GANTC 1 cut(s) 294
HpaI GTTAAC 1 cut(s) 219
HpaII CCGG 7 cut(s) 60, 123, 162, 230, 282, 414, 420
Hpy166II GTNNAC 3 cut(s) 199, 219, 327
Hpy188I TCNGA 2 cut(s) 129, 191
Hpy8I GTNNAC 3 cut(s) 199, 219, 327
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 4 cut(s) 100, 139, 196, 514
HpyCH4IV ACGT 3 cut(s) 201, 452, 461
HpyCH4V TGCA 3 cut(s) 152, 479, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 158
HpyF3I CTNAG 2 cut(s) 183, 241
HpySE526I ACGT 3 cut(s) 201, 452, 461
Hsp92II CATG 2 cut(s) 113, 308
KpnI GGTACC 1 cut(s) 420
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 219
Kzo9I GATC 2 cut(s) 211, 232
Lsp1109I GCAGC 2 cut(s) 360, 463
LweI GCATC 2 cut(s) 463, 491
MaeI CTAG 1 cut(s) 526
MaeII ACGT 3 cut(s) 201, 452, 461
MaeIII GTNAC 3 cut(s) 202, 274, 508
MalI GATC 2 cut(s) 213, 234
MboI GATC 2 cut(s) 211, 232
MboII GAAGA 1 cut(s) 97
MflI RGATCY 1 cut(s) 232
MlyI GAGTC 1 cut(s) 288
MnlI CCTC 4 cut(s) 251, 379, 391, 434
MroNI GCCGGC 1 cut(s) 161
MseI TTAA 1 cut(s) 218
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 7 cut(s) 60, 123, 162, 230, 282, 414, 420
MspR9I CCNGG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 56, 158
NaeI GCCGGC 1 cut(s) 163
NciI CCSGG 1 cut(s) 283
NdeII GATC 2 cut(s) 211, 232
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 2 cut(s) 113, 308
NlaIV GGNNCC 2 cut(s) 370, 418
NmuCI GTSAC 2 cut(s) 202, 274
NspV TTCGAA 1 cut(s) 456
OliI CACNNNNGTG 1 cut(s) 305
PcsI WCGNNNNNNNCGW 2 cut(s) 453, 458
PdiI GCCGGC 1 cut(s) 163
PkrI GCNGC 3 cut(s) 49, 350, 478
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
Ppu21I YACGTR 1 cut(s) 202
Psp1406I AACGTT 1 cut(s) 461
PspN4I GGNNCC 2 cut(s) 370, 418
PspPI GGNCC 2 cut(s) 125, 174
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 2 cut(s) 341, 418
RsaNI GTAC 2 cut(s) 340, 417
RseI CAYNNNNRTG 1 cut(s) 305
Rsr2I CGGWCCG 1 cut(s) 125
RsrII CGGWCCG 1 cut(s) 125
SaqAI TTAA 1 cut(s) 218
SatI GCNGC 3 cut(s) 48, 349, 477
Sau3AI GATC 2 cut(s) 211, 232
Sau96I GGNCC 2 cut(s) 125, 174
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 283
SetI ASST 8 cut(s) 44, 81, 157, 179, 204, 402, 455, 464
SfaNI GCATC 2 cut(s) 463, 491
SfuI TTCGAA 1 cut(s) 456
SinI GGWCC 2 cut(s) 125, 174
SmiMI CAYNNNNRTG 1 cut(s) 305
SsiI CCGC 3 cut(s) 48, 222, 389
SspMI CTAG 1 cut(s) 526
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 4 cut(s) 100, 139, 196, 514
TaiI ACGT 3 cut(s) 204, 455, 464
TaqI TCGA 4 cut(s) 317, 384, 430, 456
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 50
Tru1I TTAA 1 cut(s) 218
Tru9I TTAA 1 cut(s) 218
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 2 cut(s) 202, 274
TseI GCWGC 2 cut(s) 348, 476
Tsp45I GTSAC 2 cut(s) 202, 274
TspDTI ATGAA 1 cut(s) 4
TspGWI ACGGA 2 cut(s) 204, 306
TspRI CASTG 1 cut(s) 519
VpaK11BI GGWCC 2 cut(s) 125, 174
XspI CTAG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.