Rorug02G0468200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001118
Physical Location & Seq
Forward (+)
59908556 .. 59909041
486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0468200.1

Sequence Viewer

Length: 486 bp
ATGCTAGACTACGTATACTCTTTGAATCTCAATGAGCTTCTCAGTTATGATGATCAAGATGATGATGATTTAGTACCCACCGAGCTCAATTCTGTTTTTAGTGAACTGGGAGCGATTAATGTTCAAGGGATGTTTCACTGCAATGGCTTGTTGTATTTCCGTTTACTTGGAAAAAGTTATTTGGTTAACCCGGCAACCAGGGAATCCAATAAACTTCCTAGGGCACCAAGACTGCAAGGGGAGTACGGCCCTTTTCCAAATAACTTATATGGGTTTGGATTTGACTACTCCATTGATGATTATAAGGTGGTTAATGGGCAGATCTGTTGGCAGAATGGTGATCTTGTAGTTCAATTTAGTGTCTATACATTGAAAGCCGGTTCATGGCGGGTGATTGAGAGGCAATTTCCCTACAGTATACCTGAATATTCACAGTATCCGCCTAGAAATTTCACTGGGATTTTGCTGAATGGAGGTCTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

161

Amino Acids

18.43

Weight (kDa)

4.64

Isoelectric Point (pI)

26.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 12 - 158 2.3e-10 F-box associated beta propeller domain
FBA_3 PF08268 41 - 158 6.8e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 223
AccI GTMKAC 2 cut(s) 15, 418
AciI CCGC 2 cut(s) 388, 440
AcsI RAATTY 1 cut(s) 448
AfaI GTAC 2 cut(s) 75, 245
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 4 cut(s) 25, 125, 353, 373
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 2 cut(s) 37, 85
AluI AGCT 2 cut(s) 37, 85
Alw21I GWGCWC 1 cut(s) 87
AoxI GGCC 1 cut(s) 247
ApoI RAATTY 1 cut(s) 448
AseI ATTAAT 1 cut(s) 117
AspA2I CCTAGG 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 350, 403
AvrII CCTAGG 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 447
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 1 cut(s) 191
BfaI CTAG 3 cut(s) 5, 219, 444
BfmI CTRYAG 1 cut(s) 412
BfuI GTATCC 1 cut(s) 447
BglII AGATCT 1 cut(s) 321
BlnI CCTAGG 1 cut(s) 218
Bme1390I CCNGG 2 cut(s) 191, 199
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 2 cut(s) 191, 199
BmrI ACTGGG 2 cut(s) 116, 465
BmuI ACTGGG 2 cut(s) 116, 465
BpiI GAAGAC 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 198, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 111, 460
Bse3DI GCAATG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 2 cut(s) 198, 218
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 55
BseNI ACTGG 2 cut(s) 111, 460
BseSI GKGCMC 1 cut(s) 226
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 2 cut(s) 191, 378
BslI CCNNNNNNNGG 1 cut(s) 384
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 87, 226
Bsp143I GATC 3 cut(s) 52, 321, 340
BspACI CCGC 2 cut(s) 388, 440
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 148
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 2 cut(s) 111, 460
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 198, 218
BssMI GATC 3 cut(s) 52, 321, 340
BssNAI GTATAC 2 cut(s) 16, 419
BssT1I CCWWGG 1 cut(s) 218
Bst1107I GTATAC 2 cut(s) 16, 419
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 2 cut(s) 416, 435
BstBAI YACGTR 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 135
BstKTI GATC 3 cut(s) 55, 324, 343
BstMBI GATC 3 cut(s) 52, 321, 340
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 2 cut(s) 189, 197
BstSFI CTRYAG 1 cut(s) 412
BstSLI GKGCMC 1 cut(s) 226
BstSNI TACGTA 1 cut(s) 13
BstV2I GAAGAC 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BstZ17I GTATAC 2 cut(s) 16, 419
BsuI GTATCC 1 cut(s) 447
BsuRI GGCC 1 cut(s) 249
BtsCI GGATG 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 453
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 74, 244
CviAII CATG 1 cut(s) 384
CviJI RGCY 5 cut(s) 37, 85, 147, 249, 377
CviKI_1 RGCY 5 cut(s) 37, 85, 147, 249, 377
CviQI GTAC 2 cut(s) 74, 244
DdeI CTNAG 1 cut(s) 41
DpnI GATC 3 cut(s) 54, 323, 342
DpnII GATC 3 cut(s) 52, 321, 340
EciI GGCGGA 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 85
Eco105I TACGTA 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 218
Eco24I GRGCYC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 197
EcoT14I CCWWGG 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 1 cut(s) 387
FaiI YATR 8 cut(s) 16, 48, 268, 270, 303, 366, 385, 419
FatI CATG 1 cut(s) 383
FauI CCCGC 1 cut(s) 381
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 2 cut(s) 15, 418
FokI GGATG 1 cut(s) 142
FriOI GRGCYC 1 cut(s) 87
FspBI CTAG 3 cut(s) 5, 219, 444
HaeIII GGCC 1 cut(s) 249
HapII CCGG 2 cut(s) 191, 378
Hin1II CATG 1 cut(s) 387
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 2 cut(s) 25, 203
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 2 cut(s) 191, 378
HphI GGTGA 2 cut(s) 350, 403
Hpy166II GTNNAC 5 cut(s) 16, 104, 164, 187, 419
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 5 cut(s) 16, 104, 164, 187, 419
HpyCH4III ACNGT 2 cut(s) 416, 435
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 2 cut(s) 141, 235
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 1 cut(s) 387
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 1 cut(s) 187
Kzo9I GATC 3 cut(s) 52, 321, 340
LmnI GCTCC 1 cut(s) 110
LpnPI CCDG 7 cut(s) 92, 184, 204, 211, 391, 435, 441
MaeI CTAG 3 cut(s) 5, 219, 444
MaeII ACGT 1 cut(s) 12
MalI GATC 3 cut(s) 54, 323, 342
MboI GATC 3 cut(s) 52, 321, 340
MboII GAAGA 1 cut(s) 470
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 2 cut(s) 87, 226
MluCI AATT 4 cut(s) 88, 353, 404, 448
MnlI CCTC 2 cut(s) 393, 467
MseI TTAA 3 cut(s) 117, 186, 312
MslI CAYNNNNRTG 1 cut(s) 141
MspI CCGG 2 cut(s) 191, 378
MspR9I CCNGG 2 cut(s) 191, 199
MvaI CCWGG 1 cut(s) 199
NciI CCSGG 1 cut(s) 191
NdeII GATC 3 cut(s) 52, 321, 340
NlaIII CATG 1 cut(s) 387
NlaIV GGNNCC 1 cut(s) 225
PfeI GAWTC 2 cut(s) 25, 203
Ppu21I YACGTR 1 cut(s) 13
PshBI ATTAAT 1 cut(s) 117
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 1 cut(s) 248
PsuI RGATCY 1 cut(s) 321
RsaI GTAC 2 cut(s) 75, 245
RsaNI GTAC 2 cut(s) 74, 244
RseI CAYNNNNRTG 1 cut(s) 141
SacI GAGCTC 1 cut(s) 87
SaqAI TTAA 3 cut(s) 117, 186, 312
Sau3AI GATC 3 cut(s) 52, 321, 340
Sau96I GGNCC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 191, 199
SduI GDGCHC 2 cut(s) 87, 226
SetI ASST 6 cut(s) 15, 39, 87, 309, 424, 478
SfcI CTRYAG 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 141
SnaBI TACGTA 1 cut(s) 13
Sse9I AATT 4 cut(s) 88, 353, 404, 448
SsiI CCGC 2 cut(s) 388, 440
SspI AATATT 1 cut(s) 428
SspMI CTAG 3 cut(s) 5, 219, 444
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 189, 197
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 2 cut(s) 416, 435
TaiI ACGT 1 cut(s) 15
TasI AATT 4 cut(s) 88, 353, 404, 448
TfiI GAWTC 2 cut(s) 25, 203
Tru1I TTAA 3 cut(s) 117, 186, 312
Tru9I TTAA 3 cut(s) 117, 186, 312
TscAI CASTG 2 cut(s) 143, 460
TspDTI ATGAA 2 cut(s) 372, 470
TspGWI ACGGA 1 cut(s) 149
TspRI CASTG 2 cut(s) 143, 460
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 1 cut(s) 448
XmaJI CCTAGG 1 cut(s) 218
XmiI GTMKAC 2 cut(s) 15, 418
XspI CTAG 3 cut(s) 5, 219, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.