Rmu_sc0002267.1_g000009

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002267.1
OG0001118
Physical Location & Seq
Forward (+)
30186 .. 33878
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002267.1_g000009.1.cds

Sequence Viewer

Length: 681 bp
atggcattacagttcaattatcattccctactattggtcgtggttctggccacgactacattcttagacatagccactagcagtatagagttgaaagaaaccaagctatccttgtatttccaggatttctctgccggtccaaatacctcagcagtaccaattgcaggtattgcaggcaagctatggagggtcgatcagtttggaacagttatcgtcacggatgaccggctaactgtagccccgagtccgaaatcagcaacggtgggccgaggccaaggcttggttgtaacatcatcactagatggacacaatgcccatgtcttgttatcgattgtgttcaccaacaggaggtacaatggcagcactctagagatacaaggaactagtaggcaattcgaggcggttagagaggtttcggtcgtatcaggcaccgggaagtttcgaaaggaggagctgtgcagccgcgcaggtgaagtgaagagagagagagcagtctgggcggcgtgtcgatctgcctcacggcctcggcgtggtctcgatctgcatctcggcctcggcgtggtcttgatctgcgtcacagcgtctgggcgtcacagcatcggcggaggaggcggcccagctatggagaaactgagcagcatgaatcatgtgtgttctgtgttcagatcgactggagattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

226

Amino Acids

24.16

Weight (kDa)

9.54

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 2 cut(s) 155, 458
AccB1I GGYRCC 1 cut(s) 428
AccB7I CCANNNNNTGG 1 cut(s) 280
AccII CGCG 1 cut(s) 465
AciI CCGC 5 cut(s) 401, 463, 500, 603, 612
AcoI YGGCCR 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 589
AfaI GTAC 2 cut(s) 156, 353
AfiI CCNNNNNNNGG 7 cut(s) 34, 164, 280, 348, 530, 559, 622
AgsI TTSAA 2 cut(s) 16, 94
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 4 cut(s) 106, 181, 454, 620
AluI AGCT 4 cut(s) 106, 181, 454, 620
Alw26I GTCTC 1 cut(s) 539
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 6 cut(s) 48, 265, 271, 521, 550, 613
ApeKI GCWGC 3 cut(s) 360, 459, 636
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 137, 265, 614
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 2 cut(s) 331, 482
AsuII TTCGAA 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 1 cut(s) 137
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 428
BbvCI CCTCAGC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 372, 471, 648
BccI CCATC 1 cut(s) 296
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 1 cut(s) 122
BcnI CCSGG 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 539
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 4 cut(s) 78, 299, 368, 384
BfmI CTRYAG 1 cut(s) 234
BfuAI ACCTGC 2 cut(s) 155, 458
BisI GCNGC 6 cut(s) 361, 460, 463, 501, 613, 637
BlsI GCNGC 6 cut(s) 362, 461, 464, 502, 614, 638
Bme1390I CCNGG 2 cut(s) 122, 433
Bme18I GGWCC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 137, 265, 614
BmiI GGNNCC 1 cut(s) 430
BmrFI CCNGG 2 cut(s) 122, 433
BmsI GCATC 2 cut(s) 553, 606
Bpu10I CCTNAGC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 442
BpuMI CCSGG 1 cut(s) 433
Bsa29I ATCGAT 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 589
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 4 cut(s) 268, 274, 524, 553
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 7 cut(s) 34, 164, 280, 348, 530, 559, 622
Bse118I RCCGGY 2 cut(s) 134, 225
Bse1I ACTGG 1 cut(s) 676
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 1 cut(s) 122
BseCI ATCGAT 1 cut(s) 329
BseDI CCNNGG 4 cut(s) 268, 274, 524, 553
BseGI GGATG 1 cut(s) 226
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 7 cut(s) 34, 164, 280, 348, 530, 559, 622
BseMII CTCAG 2 cut(s) 162, 623
BseNI ACTGG 1 cut(s) 676
BseRI GAGGAG 2 cut(s) 464, 621
BseXI GCAGC 3 cut(s) 372, 471, 648
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 478
Bsh1236I CGCG 1 cut(s) 465
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 6 cut(s) 50, 267, 273, 523, 552, 615
BshNI GGYRCC 1 cut(s) 428
BshVI ATCGAT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 3 cut(s) 135, 226, 432
BslI CCNNNNNNNGG 7 cut(s) 34, 164, 280, 348, 530, 559, 622
BsmAI GTCTC 1 cut(s) 539
BsnI GGCC 6 cut(s) 50, 267, 273, 523, 552, 615
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 442
Bsp143I GATC 5 cut(s) 193, 509, 538, 567, 665
BspACI CCGC 5 cut(s) 401, 463, 500, 603, 612
BspANI GGCC 6 cut(s) 50, 267, 273, 523, 552, 615
BspCNI CTCAG 2 cut(s) 161, 624
BspDI ATCGAT 1 cut(s) 329
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 1 cut(s) 430
BspMI ACCTGC 2 cut(s) 155, 458
BspT104I TTCGAA 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 539
BsrFI RCCGGY 2 cut(s) 134, 225
BsrI ACTGG 1 cut(s) 676
BssAI RCCGGY 2 cut(s) 134, 225
BssECI CCNNGG 4 cut(s) 268, 274, 524, 553
BssMI GATC 5 cut(s) 193, 509, 538, 567, 665
BssNI GRCGYC 1 cut(s) 589
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 4 cut(s) 12, 208, 235, 262
Bst6I CTCTTC 1 cut(s) 473
BstACI GRCGYC 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 170
BstBI TTCGAA 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 175, 179
BstDEI CTNAG 3 cut(s) 64, 148, 632
BstF5I GGATG 1 cut(s) 226
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 5 cut(s) 196, 512, 541, 570, 668
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 5 cut(s) 193, 509, 538, 567, 665
BstMCI CGRYCG 1 cut(s) 420
BstMWI GCNNNNNNNGC 3 cut(s) 170, 497, 609
BstNI CCWGG 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 120, 431
BstSFI CTRYAG 1 cut(s) 234
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 3 cut(s) 372, 471, 648
Bsu15I ATCGAT 1 cut(s) 329
BsuRI GGCC 6 cut(s) 50, 267, 273, 523, 552, 615
BsuTUI ATCGAT 1 cut(s) 329
BtsCI GGATG 1 cut(s) 226
BveI ACCTGC 2 cut(s) 155, 458
Cac8I GCNNGC 2 cut(s) 175, 179
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 1 cut(s) 467
Cfr10I RCCGGY 2 cut(s) 134, 225
Cfr13I GGNCC 3 cut(s) 137, 265, 614
ClaI ATCGAT 1 cut(s) 329
CseI GACGC 3 cut(s) 562, 570, 578
Csp6I GTAC 2 cut(s) 155, 352
CviAII CATG 3 cut(s) 317, 640, 647
CviQI GTAC 2 cut(s) 155, 352
DdeI CTNAG 3 cut(s) 64, 148, 632
DpnI GATC 5 cut(s) 195, 511, 540, 569, 667
DpnII GATC 5 cut(s) 193, 509, 538, 567, 665
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 473
EciI GGCGGA 1 cut(s) 618
Eco130I CCWWGG 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 3 cut(s) 320, 643, 650
FaiI YATR 7 cut(s) 71, 86, 184, 318, 623, 641, 648
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 3 cut(s) 316, 639, 646
Fnu4HI GCNGC 6 cut(s) 361, 460, 463, 501, 613, 637
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 6 cut(s) 361, 460, 463, 501, 613, 637
FspBI CTAG 4 cut(s) 78, 299, 368, 384
GlaI GCGC 1 cut(s) 466
GluI GCNGC 6 cut(s) 361, 460, 463, 501, 613, 637
GsaI CCCAGC 1 cut(s) 620
HaeIII GGCC 6 cut(s) 50, 267, 273, 523, 552, 615
HapII CCGG 3 cut(s) 135, 226, 432
HgaI GACGC 3 cut(s) 562, 570, 578
HhaI GCGC 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 589
Hin1II CATG 3 cut(s) 320, 643, 650
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HinfI GANTC 2 cut(s) 244, 643
HpaII CCGG 3 cut(s) 135, 226, 432
HphI GGTGA 2 cut(s) 331, 482
Hpy166II GTNNAC 1 cut(s) 339
Hpy188I TCNGA 2 cut(s) 249, 665
Hpy188III TCNNGA 3 cut(s) 368, 536, 565
Hpy8I GTNNAC 1 cut(s) 339
HpyCH4III ACNGT 4 cut(s) 12, 208, 235, 262
HpyCH4V TGCA 4 cut(s) 164, 173, 459, 544
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 497, 609
HpyF3I CTNAG 3 cut(s) 64, 148, 632
Hsp92I GRCGYC 1 cut(s) 589
Hsp92II CATG 3 cut(s) 320, 643, 650
HspAI GCGC 1 cut(s) 465
Kzo9I GATC 5 cut(s) 193, 509, 538, 567, 665
LmnI GCTCC 1 cut(s) 451
Lsp1109I GCAGC 3 cut(s) 372, 471, 648
LweI GCATC 2 cut(s) 553, 606
MaeI CTAG 4 cut(s) 78, 299, 368, 384
MaeIII GTNAC 4 cut(s) 214, 286, 574, 590
MalI GATC 5 cut(s) 195, 511, 540, 569, 667
MboI GATC 5 cut(s) 193, 509, 538, 567, 665
MboII GAAGA 1 cut(s) 490
MfeI CAATTG 1 cut(s) 159
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 3 cut(s) 16, 159, 392
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 1 cut(s) 253
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
Msp20I TGGCCA 1 cut(s) 50
MspI CCGG 3 cut(s) 135, 226, 432
MspR9I CCNGG 2 cut(s) 122, 433
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 3 cut(s) 170, 497, 609
NciI CCSGG 1 cut(s) 433
NdeII GATC 5 cut(s) 193, 509, 538, 567, 665
NlaIII CATG 3 cut(s) 320, 643, 650
NlaIV GGNNCC 1 cut(s) 430
NmeAIII GCCGAG 4 cut(s) 293, 505, 528, 534
NmuCI GTSAC 3 cut(s) 214, 574, 590
NspV TTCGAA 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 2 cut(s) 526, 555
PfeI GAWTC 1 cut(s) 643
PflMI CCANNNNNTGG 1 cut(s) 280
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 6 cut(s) 362, 461, 464, 502, 614, 638
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 120
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 430
PspPI GGNCC 3 cut(s) 137, 265, 614
PstNI CAGNNNCTG 1 cut(s) 584
RsaI GTAC 2 cut(s) 156, 353
RsaNI GTAC 2 cut(s) 155, 352
SatI GCNGC 6 cut(s) 361, 460, 463, 501, 613, 637
Sau3AI GATC 5 cut(s) 193, 509, 538, 567, 665
Sau96I GGNCC 3 cut(s) 137, 265, 614
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 122, 433
SetI ASST 9 cut(s) 108, 149, 169, 183, 353, 414, 456, 472, 622
SfaNI GCATC 2 cut(s) 553, 606
SfcI CTRYAG 1 cut(s) 234
SfuI TTCGAA 1 cut(s) 442
SinI GGWCC 1 cut(s) 137
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 3 cut(s) 16, 159, 392
SsiI CCGC 5 cut(s) 401, 463, 500, 603, 612
SspMI CTAG 4 cut(s) 78, 299, 368, 384
StyD4I CCNGG 2 cut(s) 120, 431
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 4 cut(s) 12, 208, 235, 262
TaqI TCGA 7 cut(s) 192, 329, 396, 442, 508, 537, 668
TaqII GACCGA 1 cut(s) 406
TasI AATT 3 cut(s) 16, 159, 392
TauI GCSGC 3 cut(s) 465, 503, 615
TfiI GAWTC 1 cut(s) 643
TseFI GTSAC 3 cut(s) 214, 574, 590
TseI GCWGC 3 cut(s) 360, 459, 636
Tsp45I GTSAC 3 cut(s) 214, 574, 590
TspDTI ATGAA 1 cut(s) 656
TspGWI ACGGA 1 cut(s) 233
Van91I CCANNNNNTGG 1 cut(s) 280
VpaK11BI GGWCC 1 cut(s) 137
XbaI TCTAGA 1 cut(s) 367
XspI CTAG 4 cut(s) 78, 299, 368, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.