Rh2BG552000

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001118
Physical Location & Seq
Reverse (-)
76051707 .. 76052063
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG552000.1

Sequence Viewer

Length: 357 bp
ATGGAGGGTCGATCAGTTTGGAACAGTTATCTCACGGATGACCGGCTAACTGTAGCCCCGAGTCCGAAATCAGCAACGGTGGGCCGAGGCCAAGGCTTGGTTGTAACATCATCACTAGATGGACGCAATGCCCATGTCTTGTTATCGATTGTGTTCACCAACAGGAGGTACAATGGCAGCACTCTAGAGAGACAAGGAACTAGTAGGCAATTCGAGGCGGTTAGAGAGGTTTCGGTCGTATCAGGCACCGGGAAGTTTCGGTTTGCTAGAGGATATGCTACATTCGAAACTTATTCTGTGGACCCTACAAATGCATACGCAGTTATACGATTCAATATTACTGTGCAACACAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

118

Amino Acids

13.08

Weight (kDa)

10.18

Isoelectric Point (pI)

29.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 10 - 115 9.3e-34 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 97
AciI CCGC 1 cut(s) 218
AfaI GTAC 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 97, 165
AgsI TTSAA 1 cut(s) 334
AhlI ACTAGT 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 184
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 2 cut(s) 82, 88
ApeKI GCWGC 1 cut(s) 177
AspS9I GGNCC 2 cut(s) 82, 301
AsuC2I CCSGG 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 148
AsuII TTCGAA 1 cut(s) 285
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 189
BccI CCATC 1 cut(s) 113
BcnI CCSGG 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 184
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 4 cut(s) 116, 185, 201, 267
BfmI CTRYAG 1 cut(s) 51
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
Bme1390I CCNGG 1 cut(s) 250
Bme18I GGWCC 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 82, 301
BmiI GGNNCC 2 cut(s) 247, 303
BmrFI CCNGG 1 cut(s) 250
Bpu14I TTCGAA 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 250
Bsa29I ATCGAT 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 85, 91
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 165
Bse118I RCCGGY 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 85, 91
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 97, 165
BseMI GCAATG 1 cut(s) 133
BseXI GCAGC 1 cut(s) 189
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 2 cut(s) 84, 90
BshNI GGYRCC 1 cut(s) 245
BshVI ATCGAT 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 2 cut(s) 43, 249
BslI CCNNNNNNNGG 2 cut(s) 97, 165
BsmAI GTCTC 1 cut(s) 184
BsnI GGCC 2 cut(s) 84, 90
BsoBI CYCGRG 1 cut(s) 58
Bsp119I TTCGAA 1 cut(s) 285
Bsp143I GATC 1 cut(s) 11
BspACI CCGC 1 cut(s) 218
BspANI GGCC 2 cut(s) 84, 90
BspDI ATCGAT 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 247, 303
BspT104I TTCGAA 1 cut(s) 285
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 133
BsrFI RCCGGY 1 cut(s) 42
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 2 cut(s) 85, 91
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 91
Bst4CI ACNGT 4 cut(s) 26, 52, 79, 343
BstBI TTCGAA 1 cut(s) 285
BstF5I GGATG 1 cut(s) 43
BstKTI GATC 1 cut(s) 14
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 1 cut(s) 11
BstMCI CGRYCG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 248
BstSFI CTRYAG 1 cut(s) 51
BstV1I GCAGC 1 cut(s) 189
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 2 cut(s) 84, 90
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 1 cut(s) 43
Cfr10I RCCGGY 1 cut(s) 42
Cfr13I GGNCC 2 cut(s) 82, 301
ClaI ATCGAT 1 cut(s) 146
CseI GACGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 169
CviAII CATG 1 cut(s) 134
CviJI RGCY 5 cut(s) 46, 56, 84, 90, 96
CviKI_1 RGCY 5 cut(s) 46, 56, 84, 90, 96
CviQI GTAC 1 cut(s) 169
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 1 cut(s) 137
FaiI YATR 4 cut(s) 135, 276, 316, 326
FatI CATG 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 50
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 4 cut(s) 116, 185, 201, 267
GluI GCNGC 1 cut(s) 178
HaeIII GGCC 2 cut(s) 84, 90
HapII CCGG 2 cut(s) 43, 249
HgaI GACGC 1 cut(s) 132
Hin1II CATG 1 cut(s) 137
HinfI GANTC 2 cut(s) 61, 330
HpaII CCGG 2 cut(s) 43, 249
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 156, 301
Hpy188I TCNGA 1 cut(s) 66
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 2 cut(s) 156, 301
HpyCH4III ACNGT 4 cut(s) 26, 52, 79, 343
HpyCH4V TGCA 2 cut(s) 314, 346
Hsp92II CATG 1 cut(s) 137
Kzo9I GATC 1 cut(s) 11
LpnPI CCDG 4 cut(s) 56, 148, 228, 262
Lsp1109I GCAGC 1 cut(s) 189
MaeI CTAG 4 cut(s) 116, 185, 201, 267
MaeIII GTNAC 1 cut(s) 103
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MluCI AATT 1 cut(s) 209
MlyI GAGTC 1 cut(s) 70
MnlI CCTC 5 cut(s) 80, 159, 208, 220, 263
Mph1103I ATGCAT 1 cut(s) 316
MspI CCGG 2 cut(s) 43, 249
MspR9I CCNGG 1 cut(s) 250
NciI CCSGG 1 cut(s) 250
NdeII GATC 1 cut(s) 11
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 2 cut(s) 247, 303
NmeAIII GCCGAG 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 316
NspV TTCGAA 1 cut(s) 285
PfeI GAWTC 1 cut(s) 330
PflMI CCANNNNNTGG 1 cut(s) 97
PkrI GCNGC 1 cut(s) 179
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
PspN4I GGNNCC 2 cut(s) 247, 303
PspPI GGNCC 2 cut(s) 82, 301
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 2 cut(s) 82, 301
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 250
SetI ASST 2 cut(s) 170, 231
SfcI CTRYAG 1 cut(s) 51
SfuI TTCGAA 1 cut(s) 285
SinI GGWCC 1 cut(s) 301
SpeI ACTAGT 1 cut(s) 200
Sse9I AATT 1 cut(s) 209
SsiI CCGC 1 cut(s) 218
SspI AATATT 1 cut(s) 337
SspMI CTAG 4 cut(s) 116, 185, 201, 267
StyD4I CCNGG 1 cut(s) 248
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 4 cut(s) 26, 52, 79, 343
TaqI TCGA 4 cut(s) 10, 146, 213, 285
TaqII GACCGA 1 cut(s) 223
TasI AATT 1 cut(s) 209
TfiI GAWTC 1 cut(s) 330
TseI GCWGC 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 50
Van91I CCANNNNNTGG 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 301
XbaI TCTAGA 1 cut(s) 184
XspI CTAG 4 cut(s) 116, 185, 201, 267
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.