Rw2G044630

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001118
Physical Location & Seq
Reverse (-)
71270013 .. 71282273
12261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044630.1

Sequence Viewer

Length: 495 bp
ATGGCATTAAACTATATACCCAAGTTACTGTTGGTCCTGGTTCTAACCATGAATACATTTTCCGCCATAGGCGGTAGCTGTGAAGAGTTCAAAGAAACCCACATGTCCATGTACCTTCAGGACTTCATTGCCGTCCGAAATCGATCAGCAACGGTGGGCCAAGGCCAAGGCTTGGTTGTAACATCATCACTAGATGGACGCAATGCCCATGTCTTGTTATCGATTGTGTTCACCAACAGGAGGTACAATGGCAGCACTCTAGAGATACAAGGAACTAGTAGGCAATTCGAGGCGGTTAGAGAGGTTTCGGTCGTATCAGGCACCGGGAAGTTTCGGTTTGCTAGAGGATATGCTACATTCGAAACTTATTCTGTGGACCCTACAAATTTATACGCAGTAAAACCCTCATATAACACTTTTTGGTACCTACGATTTTTTTTAATTCAAATGGTACATGAATTATTGCACTGTTACCAAATACGGTACCTCCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

164

Amino Acids

18.76

Weight (kDa)

9.36

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 47 - 133 1.7e-26 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 423, 483
AccB1I GGYRCC 3 cut(s) 320, 423, 483
AccB7I CCANNNNNTGG 1 cut(s) 172
AciI CCGC 3 cut(s) 63, 72, 293
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 5 cut(s) 113, 245, 425, 453, 485
AfiI CCNNNNNNNGG 2 cut(s) 172, 240
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 2 cut(s) 91, 446
AhlI ACTAGT 1 cut(s) 275
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 1 cut(s) 78
AluI AGCT 1 cut(s) 78
AoxI GGCC 2 cut(s) 157, 163
ApeKI GCWGC 1 cut(s) 252
ApoI RAATTY 1 cut(s) 385
Asp718I GGTACC 2 cut(s) 423, 483
AspS9I GGNCC 3 cut(s) 34, 157, 376
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 223
AsuII TTCGAA 1 cut(s) 360
AvaII GGWCC 2 cut(s) 34, 376
BaeI ACNNNNGTAYC 1 cut(s) 475
BanI GGYRCC 3 cut(s) 320, 423, 483
BbvI GCAGC 1 cut(s) 264
BccI CCATC 1 cut(s) 188
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 38
BcnI CCSGG 1 cut(s) 325
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 4 cut(s) 191, 260, 276, 342
BisI GCNGC 1 cut(s) 253
BlsI GCNGC 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 38, 325
Bme18I GGWCC 2 cut(s) 34, 376
BmgT120I GGNCC 3 cut(s) 34, 157, 376
BmiI GGNNCC 4 cut(s) 322, 378, 425, 485
BmrFI CCNGG 2 cut(s) 38, 325
Bpu14I TTCGAA 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 325
Bsa29I ATCGAT 2 cut(s) 142, 221
BsaJI CCNNGG 2 cut(s) 160, 166
BsaXI ACNNNNNCTCC 1 cut(s) 471
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 240
Bse3DI GCAATG 2 cut(s) 126, 208
BseBI CCWGG 1 cut(s) 38
BseCI ATCGAT 2 cut(s) 142, 221
BseDI CCNNGG 2 cut(s) 160, 166
BseLI CCNNNNNNNGG 2 cut(s) 172, 240
BseMI GCAATG 2 cut(s) 126, 208
BseXI GCAGC 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 2 cut(s) 159, 165
BshNI GGYRCC 3 cut(s) 320, 423, 483
BshVI ATCGAT 2 cut(s) 142, 221
BsiEI CGRYCG 1 cut(s) 312
BsiSI CCGG 1 cut(s) 324
BslI CCNNNNNNNGG 2 cut(s) 172, 240
BsnI GGCC 2 cut(s) 159, 165
Bsp119I TTCGAA 1 cut(s) 360
Bsp143I GATC 1 cut(s) 143
BspACI CCGC 3 cut(s) 63, 72, 293
BspANI GGCC 2 cut(s) 159, 165
BspDI ATCGAT 2 cut(s) 142, 221
BspLI GGNNCC 4 cut(s) 322, 378, 425, 485
BspT104I TTCGAA 1 cut(s) 360
BspT107I GGYRCC 3 cut(s) 320, 423, 483
BsrDI GCAATG 2 cut(s) 126, 208
BssECI CCNNGG 2 cut(s) 160, 166
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 2 cut(s) 160, 166
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 4 cut(s) 30, 154, 470, 483
Bst6I CTCTTC 1 cut(s) 78
BstBI TTCGAA 1 cut(s) 360
BstKTI GATC 1 cut(s) 146
BstMBI GATC 1 cut(s) 143
BstMCI CGRYCG 1 cut(s) 312
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 36, 323
BstV1I GCAGC 1 cut(s) 264
Bsu15I ATCGAT 2 cut(s) 142, 221
BsuRI GGCC 2 cut(s) 159, 165
BsuTUI ATCGAT 2 cut(s) 142, 221
BtsIMutI CAGTG 1 cut(s) 466
Cfr13I GGNCC 3 cut(s) 34, 157, 376
ClaI ATCGAT 2 cut(s) 142, 221
CseI GACGC 1 cut(s) 207
Csp6I GTAC 5 cut(s) 112, 244, 424, 452, 484
CviAII CATG 5 cut(s) 49, 103, 109, 209, 455
CviJI RGCY 4 cut(s) 78, 159, 165, 171
CviKI_1 RGCY 4 cut(s) 78, 159, 165, 171
CviQI GTAC 5 cut(s) 112, 244, 424, 452, 484
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 160, 166
Eco47I GGWCC 2 cut(s) 34, 376
Eco57I CTGAAG 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 2 cut(s) 160, 166
ErhI CCWWGG 2 cut(s) 160, 166
FaeI CATG 5 cut(s) 52, 106, 112, 212, 458
FatI CATG 5 cut(s) 48, 102, 108, 208, 454
Fnu4HI GCNGC 1 cut(s) 253
Fsp4HI GCNGC 1 cut(s) 253
FspBI CTAG 4 cut(s) 191, 260, 276, 342
GluI GCNGC 1 cut(s) 253
HaeIII GGCC 2 cut(s) 159, 165
HapII CCGG 1 cut(s) 324
HgaI GACGC 1 cut(s) 207
Hin1II CATG 5 cut(s) 52, 106, 112, 212, 458
HpaII CCGG 1 cut(s) 324
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 2 cut(s) 231, 376
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 2 cut(s) 119, 260
Hpy8I GTNNAC 2 cut(s) 231, 376
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 4 cut(s) 30, 154, 470, 483
HpyCH4V TGCA 1 cut(s) 466
Hsp92II CATG 5 cut(s) 52, 106, 112, 212, 458
KpnI GGTACC 2 cut(s) 427, 487
Kzo9I GATC 1 cut(s) 143
LpnPI CCDG 6 cut(s) 23, 50, 104, 223, 303, 337
Lsp1109I GCAGC 1 cut(s) 264
MaeI CTAG 4 cut(s) 191, 260, 276, 342
MaeIII GTNAC 3 cut(s) 24, 178, 470
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 1 cut(s) 95
MluCI AATT 4 cut(s) 284, 385, 441, 458
MnlI CCTC 5 cut(s) 234, 283, 295, 338, 415
MseI TTAA 3 cut(s) 8, 440, 493
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 1 cut(s) 324
MspR9I CCNGG 2 cut(s) 38, 325
MvaI CCWGG 1 cut(s) 38
NciI CCSGG 1 cut(s) 325
NdeII GATC 1 cut(s) 143
NlaIII CATG 5 cut(s) 52, 106, 112, 212, 458
NlaIV GGNNCC 4 cut(s) 322, 378, 425, 485
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 360
PciI ACATGT 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PflMI CCANNNNNTGG 1 cut(s) 172
PkrI GCNGC 1 cut(s) 254
PscI ACATGT 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 4 cut(s) 322, 378, 425, 485
PspPI GGNCC 3 cut(s) 34, 157, 376
RsaI GTAC 5 cut(s) 113, 245, 425, 453, 485
RsaNI GTAC 5 cut(s) 112, 244, 424, 452, 484
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 3 cut(s) 8, 440, 493
SatI GCNGC 1 cut(s) 253
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 3 cut(s) 34, 157, 376
ScrFI CCNGG 2 cut(s) 38, 325
SetI ASST 6 cut(s) 80, 117, 245, 306, 429, 489
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 2 cut(s) 34, 376
SmiMI CAYNNNNRTG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 275
Sse9I AATT 4 cut(s) 284, 385, 441, 458
SsiI CCGC 3 cut(s) 63, 72, 293
SspMI CTAG 4 cut(s) 191, 260, 276, 342
StyD4I CCNGG 2 cut(s) 36, 323
StyI CCWWGG 2 cut(s) 160, 166
TaaI ACNGT 4 cut(s) 30, 154, 470, 483
TaqI TCGA 4 cut(s) 142, 221, 288, 360
TaqII GACCGA 1 cut(s) 298
TasI AATT 4 cut(s) 284, 385, 441, 458
Tru1I TTAA 3 cut(s) 8, 440, 493
Tru9I TTAA 3 cut(s) 8, 440, 493
TscAI CASTG 1 cut(s) 473
TseI GCWGC 1 cut(s) 252
TspDTI ATGAA 3 cut(s) 65, 115, 471
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 1 cut(s) 473
Van91I CCANNNNNTGG 1 cut(s) 172
VpaK11BI GGWCC 2 cut(s) 34, 376
XapI RAATTY 1 cut(s) 385
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XspI CTAG 4 cut(s) 191, 260, 276, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.