RLG00000021118

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001118
Physical Location & Seq
Reverse (-)
72110122 .. 72112469
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021118

Sequence Viewer

Length: 681 bp
ATGAGTGCTAGTGCACTTTTAGTCCAAGCCAGTTATGTCAAAGAAACCCGAATGGTCTTGTACTTCCAAGACATCTCGACAGGTCCAAATGCCACAAGCATACCAATTGCAGGCATTGCCGGAAAGCTTTGGACCTTCACTCAGTTTGGAACAGTTTATGTCACTGACGACACCATGACCGAAGGTCCAAGCCCACAGTCAGCCGTAATTGGCCGGGCACAAGGCGTGTCGGTGGCAGCAGCGCTAGACGGGAGTAATGCCCTTGTCTTGGTATCGCTTGTGTTCACAAATCAAGAATACAATGGTAGCACTATAGAGATACAAGGAAACAGTAAGCAATTCGAGTCTGTCAGAGAGGTTGCAGTGGTATCAGAGGCCATAGCACACGATCAGCTGCCGCAGCCATGCACACGTTGCTTTCGACTTGCGCCAATCCCAGATGTTGCCACCACCAACCACCGTTGCATGAGAGACGCAGACCAAGATCTTGTCCTCCAAGTGCCGCAAGAACTCTTCCTCCAAGCGCCGCAACCTCTAGTTCGGAATAGTCCGACCTCTATGCTTCAGGCTCCCAATATCCGTCGAGCAACTTCAGCCGAGAAGAATCCCCCATTTGCAGGTATTCCTGGAAGGCCTAGGAATCGATTCTCGTTTGGAACAGAATTTGTCGTTAACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

227

Amino Acids

24.46

Weight (kDa)

5.7

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 16 - 124 3.9e-32 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 608
AciI CCGC 3 cut(s) 398, 503, 527
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 662
AcuI CTGAAG 2 cut(s) 548, 576
AfaI GTAC 1 cut(s) 62
AfeI AGCGCT 1 cut(s) 243
AfiI CCNNNNNNNGG 3 cut(s) 110, 268, 617
AflIII ACRYGT 1 cut(s) 410
AhdI GACNNNNNGTC 1 cut(s) 183
AjnI CCWGG 1 cut(s) 625
AloI GAACNNNNNNTCC 2 cut(s) 501, 533
AluBI AGCT 2 cut(s) 127, 394
AluI AGCT 2 cut(s) 127, 394
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 1 cut(s) 465
Alw44I GTGCAC 1 cut(s) 12
Aor51HI AGCGCT 1 cut(s) 243
AoxI GGCC 3 cut(s) 211, 375, 632
ApaLI GTGCAC 1 cut(s) 12
ApeKI GCWGC 4 cut(s) 236, 239, 394, 400
ApoI RAATTY 1 cut(s) 662
Asp700I GAANNNNTTC 1 cut(s) 644
AspA2I CCTAGG 1 cut(s) 635
AspLEI GCGC 3 cut(s) 244, 430, 526
AspS9I GGNCC 3 cut(s) 83, 132, 185
AsuC2I CCSGG 1 cut(s) 215
AvaII GGWCC 3 cut(s) 83, 132, 185
AvrII CCTAGG 1 cut(s) 635
BaeGI GKGCMC 2 cut(s) 16, 220
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 248, 251, 381, 412
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 2 cut(s) 541, 575
BciT130I CCWGG 1 cut(s) 627
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 465
BfaI CTAG 4 cut(s) 9, 245, 536, 636
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 2 cut(s) 245, 527
BfuAI ACCTGC 1 cut(s) 608
BglII AGATCT 1 cut(s) 484
BisI GCNGC 7 cut(s) 237, 240, 395, 398, 401, 503, 527
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 7 cut(s) 238, 241, 396, 399, 402, 504, 528
Bme1390I CCNGG 2 cut(s) 215, 627
Bme18I GGWCC 3 cut(s) 83, 132, 185
BmeRI GACNNNNNGTC 1 cut(s) 183
BmgT120I GGNCC 3 cut(s) 83, 132, 185
BmiI GGNNCC 1 cut(s) 570
BmrFI CCNGG 2 cut(s) 215, 627
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 1 cut(s) 643
BsaJI CCNNGG 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 268, 617
Bse1I ACTGG 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 643
BseDI CCNNGG 1 cut(s) 635
BseLI CCNNNNNNNGG 3 cut(s) 110, 268, 617
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 1 cut(s) 30
BseSI GKGCMC 2 cut(s) 16, 220
BseXI GCAGC 4 cut(s) 248, 251, 381, 412
BshFI GGCC 3 cut(s) 213, 377, 634
BshVI ATCGAT 1 cut(s) 643
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 2 cut(s) 120, 214
BslI CCNNNNNNNGG 3 cut(s) 110, 268, 617
BsmAI GTCTC 1 cut(s) 465
BsmBI CGTCTC 1 cut(s) 465
BsnI GGCC 3 cut(s) 213, 377, 634
Bsp1286I GDGCHC 2 cut(s) 16, 220
Bsp143I GATC 2 cut(s) 388, 484
BspACI CCGC 3 cut(s) 398, 503, 527
BspANI GGCC 3 cut(s) 213, 377, 634
BspCNI CTCAG 1 cut(s) 154
BspDI ATCGAT 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 570
BspMI ACCTGC 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 635
BssMI GATC 2 cut(s) 388, 484
BssT1I CCWWGG 1 cut(s) 635
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 4 cut(s) 154, 198, 332, 461
Bst6I CTCTTC 1 cut(s) 518
BstAPI GCANNNNNTGC 2 cut(s) 116, 414
BstC8I GCNNGC 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 141
BstH2I RGCGCY 2 cut(s) 245, 527
BstHHI GCGC 3 cut(s) 244, 430, 526
BstKTI GATC 2 cut(s) 391, 487
BstMAI GTCTC 1 cut(s) 465
BstMBI GATC 2 cut(s) 388, 484
BstMWI GCNNNNNNNGC 4 cut(s) 116, 400, 414, 593
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 213, 625
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 2 cut(s) 16, 220
BstV1I GCAGC 4 cut(s) 248, 251, 381, 412
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 643
BsuRI GGCC 3 cut(s) 213, 377, 634
BsuTUI ATCGAT 1 cut(s) 643
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 162, 369
BveI ACCTGC 1 cut(s) 608
Cac8I GCNNGC 1 cut(s) 112
CfoI GCGC 3 cut(s) 244, 430, 526
Cfr13I GGNCC 3 cut(s) 83, 132, 185
ClaI ATCGAT 1 cut(s) 643
CseI GACGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 61
CviAII CATG 3 cut(s) 175, 405, 466
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 1 cut(s) 141
DpnI GATC 2 cut(s) 390, 486
DpnII GATC 2 cut(s) 388, 484
DriI GACNNNNNGTC 1 cut(s) 183
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 518
Eam1105I GACNNNNNGTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 518
Eco130I CCWWGG 1 cut(s) 635
Eco147I AGGCCT 1 cut(s) 634
Eco47I GGWCC 3 cut(s) 83, 132, 185
Eco47III AGCGCT 1 cut(s) 243
Eco57I CTGAAG 2 cut(s) 548, 576
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 635
ErhI CCWWGG 1 cut(s) 635
Esp3I CGTCTC 1 cut(s) 465
FaeI CATG 3 cut(s) 178, 408, 469
FaiI YATR 9 cut(s) 36, 101, 159, 176, 314, 380, 406, 467, 560
FatI CATG 3 cut(s) 174, 404, 465
Fnu4HI GCNGC 7 cut(s) 237, 240, 395, 398, 401, 503, 527
Fsp4HI GCNGC 7 cut(s) 237, 240, 395, 398, 401, 503, 527
FspBI CTAG 4 cut(s) 9, 245, 536, 636
GlaI GCGC 3 cut(s) 243, 429, 525
GluI GCNGC 7 cut(s) 237, 240, 395, 398, 401, 503, 527
HaeII RGCGCY 2 cut(s) 245, 527
HaeIII GGCC 3 cut(s) 213, 377, 634
HapII CCGG 2 cut(s) 120, 214
HgaI GACGC 1 cut(s) 482
HhaI GCGC 3 cut(s) 244, 430, 526
Hin1II CATG 3 cut(s) 178, 408, 469
Hin6I GCGC 3 cut(s) 242, 428, 524
HinP1I GCGC 3 cut(s) 242, 428, 524
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 4 cut(s) 344, 604, 640, 645
HpaI GTTAAC 1 cut(s) 673
HpaII CCGG 2 cut(s) 120, 214
Hpy166II GTNNAC 3 cut(s) 14, 285, 673
Hpy188I TCNGA 4 cut(s) 353, 373, 543, 552
Hpy188III TCNNGA 2 cut(s) 76, 293
Hpy8I GTNNAC 3 cut(s) 14, 285, 673
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 3 cut(s) 145, 176, 624
HpyCH4III ACNGT 4 cut(s) 154, 198, 332, 461
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 6 cut(s) 14, 110, 362, 408, 465, 617
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 400, 414, 593
HpyF3I CTNAG 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 412
Hsp92II CATG 3 cut(s) 178, 408, 469
HspAI GCGC 3 cut(s) 242, 428, 524
KspAI GTTAAC 1 cut(s) 673
Kzo9I GATC 2 cut(s) 388, 484
LmnI GCTCC 1 cut(s) 574
Lsp1109I GCAGC 4 cut(s) 248, 251, 381, 412
MaeI CTAG 4 cut(s) 9, 245, 536, 636
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 1 cut(s) 160
MalI GATC 2 cut(s) 390, 486
MboI GATC 2 cut(s) 388, 484
MboII GAAGA 2 cut(s) 505, 613
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 484
MhlI GDGCHC 2 cut(s) 16, 220
MluCI AATT 4 cut(s) 105, 207, 338, 662
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 575
MnlI CCTC 6 cut(s) 349, 367, 503, 527, 543, 565
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 1 cut(s) 672
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 2 cut(s) 120, 214
MspR9I CCNGG 2 cut(s) 215, 627
MunI CAATTG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 4 cut(s) 116, 400, 414, 593
NciI CCSGG 1 cut(s) 215
NdeII GATC 2 cut(s) 388, 484
NlaIII CATG 3 cut(s) 178, 408, 469
NlaIV GGNNCC 1 cut(s) 570
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 160
PceI AGGCCT 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 604, 640, 645
PfoI TCCNGGA 1 cut(s) 625
PkrI GCNGC 7 cut(s) 238, 241, 396, 399, 402, 504, 528
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 3 cut(s) 83, 132, 185
PsuI RGATCY 1 cut(s) 484
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 7 cut(s) 237, 240, 395, 398, 401, 503, 527
Sau3AI GATC 2 cut(s) 388, 484
Sau96I GGNCC 3 cut(s) 83, 132, 185
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 2 cut(s) 215, 627
SduI GDGCHC 2 cut(s) 16, 220
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 3 cut(s) 83, 132, 185
Sse9I AATT 4 cut(s) 105, 207, 338, 662
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 3 cut(s) 398, 503, 527
SspMI CTAG 4 cut(s) 9, 245, 536, 636
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 2 cut(s) 213, 625
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 4 cut(s) 154, 198, 332, 461
TaiI ACGT 1 cut(s) 415
TaqI TCGA 5 cut(s) 77, 342, 421, 583, 643
TaqII GACCGA 1 cut(s) 194
TasI AATT 4 cut(s) 105, 207, 338, 662
TatI WGTACW 1 cut(s) 60
TauI GCSGC 3 cut(s) 400, 505, 529
TfiI GAWTC 3 cut(s) 604, 640, 645
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 2 cut(s) 169, 369
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 236, 239, 394, 400
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 2 cut(s) 169, 369
VneI GTGCAC 1 cut(s) 12
VpaK11BI GGWCC 3 cut(s) 83, 132, 185
XapI RAATTY 1 cut(s) 662
XmaJI CCTAGG 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 4 cut(s) 9, 245, 536, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.