Rh2DG557200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001118
Physical Location & Seq
Reverse (-)
78682476 .. 78682778
303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG557200.1

Sequence Viewer

Length: 303 bp
ATGGCCAGTACTCATGAGTTCAAAGAAACCCAAATGTCCCTGTACTTCCAGGACTTCACCACTGGGCCAAATCTCACGACATTACCAATTGCAGGAATTGCCGGCAAGTTTTGGACCTTTGATCAATTTGGAACAGTTTACGTCACCGACGACCCCATAACCGAAGGCCCAAGCCCTAAATCGGCACCAGTTGGTCGAGGACAGGCTTTTATTGTAACATCAGCACTAGATGGACGTAACGCCCTAGTTTTCTCTCGATTGTGTTCACAAACAAGAAGTACAATGGTAGCACTATTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

100

Amino Acids

10.91

Weight (kDa)

6.53

Isoelectric Point (pI)

31.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 10 - 83 5.8e-16 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 3 cut(s) 10, 44, 280
AfiI CCNNNNNNNGG 2 cut(s) 92, 181
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 1 cut(s) 48
AoxI GGCC 3 cut(s) 3, 65, 166
AspS9I GGNCC 3 cut(s) 65, 114, 167
AsuHPI GGTGA 2 cut(s) 49, 136
AvaII GGWCC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 184
BccI CCATC 1 cut(s) 224
BciT130I CCWGG 1 cut(s) 50
BclI TGATCA 1 cut(s) 121
BfaI CTAG 2 cut(s) 227, 245
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 3 cut(s) 65, 114, 167
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 50
BmrI ACTGGG 1 cut(s) 72
BmuI ACTGGG 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 181
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 3 cut(s) 6, 67, 188
BseBI CCWGG 1 cut(s) 50
BseLI CCNNNNNNNGG 2 cut(s) 92, 181
BseNI ACTGG 3 cut(s) 6, 67, 188
BshFI GGCC 3 cut(s) 5, 67, 168
BshNI GGYRCC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 92, 181
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 3 cut(s) 5, 67, 168
Bsp143I GATC 1 cut(s) 121
BspANI GGCC 3 cut(s) 5, 67, 168
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 186
BspT107I GGYRCC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 3 cut(s) 6, 67, 188
BssAI RCCGGY 1 cut(s) 101
BssMI GATC 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 136
BstAPI GCANNNNNTGC 1 cut(s) 98
BstC8I GCNNGC 1 cut(s) 103
BstKTI GATC 1 cut(s) 124
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BsuRI GGCC 3 cut(s) 5, 67, 168
BtsIMutI CAGTG 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 103
CciI TCATGA 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 3 cut(s) 65, 114, 167
Csp6I GTAC 3 cut(s) 9, 43, 279
CviAII CATG 1 cut(s) 14
CviJI RGCY 5 cut(s) 5, 67, 168, 174, 206
CviKI_1 RGCY 5 cut(s) 5, 67, 168, 174, 206
CviQI GTAC 3 cut(s) 9, 43, 279
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 48
FaeI CATG 1 cut(s) 17
FaiI YATR 2 cut(s) 15, 158
FaqI GGGAC 1 cut(s) 22
FatI CATG 1 cut(s) 13
FbaI TGATCA 1 cut(s) 121
FspBI CTAG 2 cut(s) 227, 245
HaeIII GGCC 3 cut(s) 5, 67, 168
HapII CCGG 1 cut(s) 102
Hin1II CATG 1 cut(s) 17
HpaII CCGG 1 cut(s) 102
HphI GGTGA 2 cut(s) 49, 136
Hpy166II GTNNAC 2 cut(s) 139, 266
Hpy188III TCNNGA 3 cut(s) 14, 76, 255
Hpy8I GTNNAC 2 cut(s) 139, 266
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 141, 235
HpyCH4V TGCA 1 cut(s) 92
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpySE526I ACGT 2 cut(s) 141, 235
Hsp92II CATG 1 cut(s) 17
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 1 cut(s) 121
LpnPI CCDG 9 cut(s) 19, 35, 48, 53, 62, 78, 115, 188, 201
MaeI CTAG 2 cut(s) 227, 245
MaeII ACGT 2 cut(s) 141, 235
MaeIII GTNAC 3 cut(s) 142, 214, 236
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MfeI CAATTG 1 cut(s) 87
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 87, 96, 125
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 1 cut(s) 191
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 101
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 98
NaeI GCCGGC 1 cut(s) 103
NdeII GATC 1 cut(s) 121
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 142
PagI TCATGA 1 cut(s) 13
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 103
PfoI TCCNGGA 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 3 cut(s) 65, 114, 167
RsaI GTAC 3 cut(s) 10, 44, 280
RsaNI GTAC 3 cut(s) 9, 43, 279
Sau3AI GATC 1 cut(s) 121
Sau96I GGNCC 3 cut(s) 65, 114, 167
ScaI AGTACT 1 cut(s) 10
ScrFI CCNGG 1 cut(s) 50
SetI ASST 3 cut(s) 119, 144, 238
SinI GGWCC 1 cut(s) 114
Sse9I AATT 3 cut(s) 87, 96, 125
SspMI CTAG 2 cut(s) 227, 245
StyD4I CCNGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 144, 238
TaqI TCGA 2 cut(s) 196, 256
TasI AATT 3 cut(s) 87, 96, 125
TatI WGTACW 3 cut(s) 8, 42, 278
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 142
TspRI CASTG 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 114
XspI CTAG 2 cut(s) 227, 245
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.