FvH4_7g09120

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001937
Physical Location & Seq
Forward (+)
8855425 .. 8859444
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09120.t2

Sequence Viewer

Length: 1686 bp
ATGGGTCATGAAATTCAGATTGAAGAAAAGAAAGGGAAGAAAAAAAAAAACAAGAACAGTAAGAAGAAAAAATTAGGAGGCCGGTTAGTCAAGGTACGCAGGAAAATGGAAGCGGAGTTCGCAGTGACTACTGCAAAGTTGATAGTACTGTCAGTATTGGTGGGTGGGTTTTTGTTCTTGTTTTCATATCTGTGTGAACTTCTGCTGTTGAGGCCTAAATCCATAAGATCCAAGCTGGAAAAGCAAGGAATTCGAGGGCCTCCACCTACATTTTTGTTGGGAAACATCCCTGAAATGAAGCGACTCCAAAGCAAGCAGCAGGGTCAGTCAATAGCAACAAGGAGTGATGATGAAGATCATGCAGCTTCTATTTCTCATCAATGGCATTTGAAACTCTTCCCTCACATAGAGCAATGGAGAAATGAACACGGTCCGATATTATTGTGTTCAGCTGCGCACATACAACAATTACTAATAACAGATATGGAAATGGTGAAGGAATTTATCTTGTGCAAGTCTATAGTCTTGGGAAATCCTTCTATTACATCCAAGGTTCTTGGGTCACTATTGGGAGAGGGCATTTTAGCGTCGAATGGATCAATGTGGGAGCATCAACGGAAAATTATTGCTCCTGAACTATACCTTGATAAGGGCATGATGAACCTGATAGTGGATTCTACAACCTCAATGCTAAGAACTTGGGAAAATGAAGTCCAAATCCAGGGAGGACTTGCTGAAATCAGAGTTGATGATCATTTAAGAAACTTATCAGCAGATGTCATCTCGAAAGCTTGTTTTAATAGCAATAATGGTCAAGGAGAAGAAATATTTTCAAAAATTAGGAAGCTGCAAAATGTTTTGGCGAAGGGATTGATGGGGATAGAAGGCTTGACCAGATGGTTTCCGACAACAGAAAATAGAAAGATCTGGAGATTAGAGAAGGAAATTGAATCACTTATATTGAAGGTGGCAAAACAACGCAGTGAGGGTTTATATGCGAAGGATTTGCTGCAAATGCTCCTGGAAGGTGCCAAAAGTTCTGGTGACTTAAATGGCCTATCACAGAATAAGTTTATTGTTGATAACTGCAAGACTATGTTCTTTGCTGGCCATGAGACCACTGCCCTTGTAACATCGTGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGTGCTGAGGTACTTGAAATCTGTGGGGAGAAGCCTCCAGATACAGATATGCTTCGACACATGAAAGTGCTCACGATGGTGATTCAGGAGGTACTGCGTCTTTACCCGCCCGTCTTCTTTGTAGCAAGAGAGGTTTTTGAAACTATAACATTCAAAAATATTACTATTCCAAAGGGTACGCTCCTTCAAATACCAATCCCCTTCTTACACCAGAACCCTGATCTATGGGGCTCCGATGCTCATAAGTTCAACCCAGAAAGATTTGCCGATGGGATTATCAAGGCTTGCAAGTCTCCACAAGCCTACATGCCATTCGGAGCAGGTTCTCGTGTATGTGTTGGTCAACACTTGGCTATGACAGAACTTAAGGTGTTTCTCTCTATGATTCTCGCAAAGTTCTGCTTCTCACTCTCACCAGCATACCAGCATTCCCCTGCGTATAATATAGTTACTGAGCCTGAACATGGTATTATTCTGCAAATGCGAAGAGCGTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

562

Amino Acids

63.31

Weight (kDa)

9.31

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 132 - 545 1.7e-83 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 1496
AccB1I GGYRCC 1 cut(s) 1028
AciI CCGC 2 cut(s) 113, 1292
AclWI GGATC 2 cut(s) 222, 604
AcoI YGGCCR 1 cut(s) 1108
AcsI RAATTY 3 cut(s) 12, 249, 500
AfaI GTAC 5 cut(s) 96, 147, 1197, 1278, 1363
AfiI CCNNNNNNNGG 4 cut(s) 649, 670, 721, 1649
AflII CTTAAG 1 cut(s) 1550
AjnI CCWGG 2 cut(s) 720, 1020
AleI CACNNNNGTG 1 cut(s) 1262
AloI GAACNNNNNNTCC 2 cut(s) 101, 133
AluBI AGCT 8 cut(s) 235, 365, 452, 791, 847, 1143, 1157, 1178
AluI AGCT 8 cut(s) 235, 365, 452, 791, 847, 1143, 1157, 1178
Alw21I GWGCWC 2 cut(s) 1186, 1257
Alw26I GTCTC 2 cut(s) 1109, 1482
AlwI GGATC 2 cut(s) 222, 604
AoxI GGCC 5 cut(s) 79, 212, 257, 1054, 1108
ApeKI GCWGC 5 cut(s) 316, 362, 452, 847, 1009
ApoI RAATTY 3 cut(s) 12, 249, 500
Asp700I GAANNNNTTC 2 cut(s) 395, 535
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 257, 431
AsuHPI GGTGA 4 cut(s) 505, 1055, 1276, 1590
AvaII GGWCC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 1110
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 1418
BauI CACGAG 3 cut(s) 1179, 1185, 1512
BbsI GAAGAC 1 cut(s) 1291
Bbv12I GWGCWC 2 cut(s) 1186, 1257
BbvCI CCTCAGC 1 cut(s) 1191
BbvI GCAGC 5 cut(s) 328, 374, 439, 834, 996
BccI CCATC 5 cut(s) 868, 891, 1170, 1255, 1448
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 988, 1022
BciT130I CCWGG 2 cut(s) 722, 1022
BclI TGATCA 1 cut(s) 751
BcoDI GTCTC 2 cut(s) 1109, 1482
BfaI CTAG 1 cut(s) 1154
BfmI CTRYAG 1 cut(s) 519
BfrI CTTAAG 1 cut(s) 1550
BfuAI ACCTGC 1 cut(s) 1496
BglII AGATCT 1 cut(s) 924
BisI GCNGC 5 cut(s) 317, 363, 453, 848, 1010
BlsI GCNGC 5 cut(s) 318, 364, 454, 849, 1011
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 722, 1022
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 257, 431
BmiI GGNNCC 2 cut(s) 1030, 1417
BmrFI CCNGG 2 cut(s) 722, 1022
BmsI GCATC 2 cut(s) 619, 1411
BpiI GAAGAC 1 cut(s) 1291
BpmI CTGGAG 2 cut(s) 949, 1206
Bpu10I CCTNAGC 1 cut(s) 1191
BsaBI GATNNNNATC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 1109
BsaJI CCNNGG 2 cut(s) 549, 721
BsaXI ACNNNNNCTCC 2 cut(s) 1143, 1173
Bsc4I CCNNNNNNNGG 4 cut(s) 649, 670, 721, 1649
Bse118I RCCGGY 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 419
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 2 cut(s) 722, 1022
BseDI CCNNGG 2 cut(s) 549, 721
BseGI GGATG 3 cut(s) 285, 545, 1162
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 4 cut(s) 649, 670, 721, 1649
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 2 cut(s) 1182, 1629
BseXI GCAGC 5 cut(s) 328, 374, 439, 834, 996
BshFI GGCC 5 cut(s) 81, 214, 259, 1056, 1110
BshNI GGYRCC 1 cut(s) 1028
BsiHKAI GWGCWC 2 cut(s) 1186, 1257
BsiSI CCGG 1 cut(s) 82
BslI CCNNNNNNNGG 4 cut(s) 649, 670, 721, 1649
BsmAI GTCTC 2 cut(s) 1109, 1482
BsmI GAATGC 1 cut(s) 1612
BsnI GGCC 5 cut(s) 81, 214, 259, 1056, 1110
Bso31I GGTCTC 1 cut(s) 1109
Bsp1286I GDGCHC 3 cut(s) 1186, 1257, 1418
Bsp143I GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
BspACI CCGC 2 cut(s) 113, 1292
BspANI GGCC 5 cut(s) 81, 214, 259, 1056, 1110
BspCNI CTCAG 2 cut(s) 1183, 1630
BspHI TCATGA 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 1030, 1417
BspMI ACCTGC 1 cut(s) 1496
BspPI GGATC 2 cut(s) 222, 604
BspQI GCTCTTC 1 cut(s) 1666
BspT107I GGYRCC 1 cut(s) 1028
BspTI CTTAAG 1 cut(s) 1550
BspTNI GGTCTC 1 cut(s) 1109
BsrDI GCAATG 1 cut(s) 419
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 549, 721
BssMI GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
BssSI CACGAG 3 cut(s) 1179, 1185, 1512
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 3 cut(s) 1179, 1185, 1512
Bst2UI CCWGG 2 cut(s) 722, 1022
Bst4CI ACNGT 3 cut(s) 59, 150, 431
Bst6I CTCTTC 2 cut(s) 401, 1666
BstAFI CTTAAG 1 cut(s) 1550
BstC8I GCNNGC 5 cut(s) 314, 1108, 1145, 1176, 1471
BstDEI CTNAG 3 cut(s) 692, 1191, 1638
BstENI CCTNNNNNAGG 1 cut(s) 647
BstF5I GGATG 3 cut(s) 285, 545, 1162
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 6 cut(s) 230, 358, 599, 754, 927, 1408
BstMAI GTCTC 2 cut(s) 1109, 1482
BstMBI GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
BstMWI GCNNNNNNNGC 4 cut(s) 119, 211, 241, 1015
BstNI CCWGG 2 cut(s) 722, 1022
BstNSI RCATGY 1 cut(s) 1495
BstSCI CCNGG 2 cut(s) 720, 1020
BstSFI CTRYAG 1 cut(s) 519
BstV1I GCAGC 5 cut(s) 328, 374, 439, 834, 996
BstV2I GAAGAC 1 cut(s) 1291
BstX2I RGATCY 2 cut(s) 227, 924
BstYI RGATCY 2 cut(s) 227, 924
BsuRI GGCC 5 cut(s) 81, 214, 259, 1056, 1110
BtsCI GGATG 3 cut(s) 285, 545, 1162
BtsI GCAGTG 3 cut(s) 129, 988, 1119
BtsIMutI CAGTG 3 cut(s) 129, 988, 1119
BveI ACCTGC 1 cut(s) 1496
Cac8I GCNNGC 5 cut(s) 314, 1108, 1145, 1176, 1471
CciI TCATGA 1 cut(s) 7
CfoI GCGC 1 cut(s) 457
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 2 cut(s) 257, 431
CpoI CGGWCCG 1 cut(s) 431
CseI GACGC 3 cut(s) 576, 1271, 1665
Csp6I GTAC 5 cut(s) 95, 146, 1196, 1277, 1362
CspCI CAANNNNNGTGG 2 cut(s) 1108, 1143
CspI CGGWCCG 1 cut(s) 431
CviAII CATG 7 cut(s) 8, 359, 655, 1112, 1246, 1492, 1649
CviQI GTAC 5 cut(s) 95, 146, 1196, 1277, 1362
DdeI CTNAG 3 cut(s) 692, 1191, 1638
DpnI GATC 6 cut(s) 229, 357, 598, 753, 926, 1407
DpnII GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
EaeI YGGCCR 1 cut(s) 1108
Eam1104I CTCTTC 2 cut(s) 401, 1666
EarI CTCTTC 2 cut(s) 401, 1666
Eco130I CCWWGG 1 cut(s) 549
Eco147I AGGCCT 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 1418
Eco31I GGTCTC 1 cut(s) 1109
Eco47I GGWCC 1 cut(s) 431
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 720, 1020
EcoT14I CCWWGG 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 1418
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 7 cut(s) 11, 362, 658, 1115, 1249, 1495, 1652
FalI AAGNNNNNCTT 2 cut(s) 1571, 1603
FatI CATG 7 cut(s) 7, 358, 654, 1111, 1245, 1491, 1648
FauI CCCGC 1 cut(s) 1299
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 5 cut(s) 317, 363, 453, 848, 1010
FokI GGATG 3 cut(s) 272, 532, 1149
FriOI GRGCYC 1 cut(s) 1418
Fsp4HI GCNGC 5 cut(s) 317, 363, 453, 848, 1010
FspBI CTAG 1 cut(s) 1154
FspI TGCGCA 1 cut(s) 456
GlaI GCGC 1 cut(s) 456
GluI GCNGC 5 cut(s) 317, 363, 453, 848, 1010
GsuI CTGGAG 2 cut(s) 949, 1206
HaeIII GGCC 5 cut(s) 81, 214, 259, 1056, 1110
HapII CCGG 1 cut(s) 82
HgaI GACGC 3 cut(s) 576, 1271, 1665
HhaI GCGC 1 cut(s) 457
Hin1II CATG 7 cut(s) 11, 362, 658, 1115, 1249, 1495, 1652
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HincII GTYRAC 1 cut(s) 1529
HindII GTYRAC 1 cut(s) 1529
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 5 cut(s) 303, 674, 950, 1267, 1570
HpaII CCGG 1 cut(s) 82
HphI GGTGA 4 cut(s) 505, 1055, 1276, 1590
Hpy166II GTNNAC 2 cut(s) 197, 1529
Hpy188I TCNGA 6 cut(s) 18, 435, 743, 906, 1420, 1502
Hpy188III TCNNGA 8 cut(s) 8, 632, 784, 928, 1166, 1223, 1258, 1271
Hpy8I GTNNAC 2 cut(s) 197, 1529
Hpy99I CGWCG 1 cut(s) 592
HpyCH4III ACNGT 3 cut(s) 59, 150, 431
HpyCH4V TGCA 8 cut(s) 134, 362, 513, 850, 1012, 1089, 1473, 1663
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 211, 241, 1015
HpyF3I CTNAG 3 cut(s) 692, 1191, 1638
Hsp92II CATG 7 cut(s) 11, 362, 658, 1115, 1249, 1495, 1652
HspAI GCGC 1 cut(s) 455
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
LguI GCTCTTC 1 cut(s) 1666
LmnI GCTCC 7 cut(s) 607, 634, 1023, 1140, 1371, 1421, 1502
Lsp1109I GCAGC 5 cut(s) 328, 374, 439, 834, 996
LweI GCATC 2 cut(s) 619, 1411
MaeI CTAG 1 cut(s) 1154
MaeIII GTNAC 6 cut(s) 124, 561, 1043, 1129, 1149, 1633
MalI GATC 6 cut(s) 229, 357, 598, 753, 926, 1407
MboI GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
MboII GAAGA 8 cut(s) 35, 49, 76, 365, 388, 833, 1291, 1683
MflI RGATCY 2 cut(s) 227, 924
MhlI GDGCHC 3 cut(s) 1186, 1257, 1418
MlsI TGGCCA 1 cut(s) 1110
MluCI AATT 8 cut(s) 12, 71, 249, 467, 500, 621, 837, 945
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 1 cut(s) 297
MmeI TCCRAC 1 cut(s) 929
Mox20I TGGCCA 1 cut(s) 1110
MroXI GAANNNNTTC 2 cut(s) 395, 535
MscI TGGCCA 1 cut(s) 1110
MseI TTAA 4 cut(s) 758, 798, 1049, 1551
MslI CAYNNNNRTG 3 cut(s) 190, 1250, 1262
Msp20I TGGCCA 1 cut(s) 1110
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 1550
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 722, 1022
Mva1269I GAATGC 1 cut(s) 1612
MvaI CCWGG 2 cut(s) 722, 1022
MwoI GCNNNNNNNGC 4 cut(s) 119, 211, 241, 1015
NdeII GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
NlaIII CATG 7 cut(s) 11, 362, 658, 1115, 1249, 1495, 1652
NlaIV GGNNCC 2 cut(s) 1030, 1417
NmuCI GTSAC 3 cut(s) 124, 561, 1043
NsbI TGCGCA 1 cut(s) 456
NspI RCATGY 1 cut(s) 1495
OliI CACNNNNGTG 1 cut(s) 1262
PagI TCATGA 1 cut(s) 7
PceI AGGCCT 1 cut(s) 214
PciSI GCTCTTC 1 cut(s) 1666
PctI GAATGC 1 cut(s) 1612
PdmI GAANNNNTTC 2 cut(s) 395, 535
PfeI GAWTC 4 cut(s) 674, 950, 1267, 1570
PfoI TCCNGGA 1 cut(s) 1020
PkrI GCNGC 5 cut(s) 318, 364, 454, 849, 1011
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 720, 1020
PspGI CCWGG 2 cut(s) 720, 1020
PspN4I GGNNCC 2 cut(s) 1030, 1417
PspPI GGNCC 2 cut(s) 257, 431
PsuI RGATCY 2 cut(s) 227, 924
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 5 cut(s) 96, 147, 1197, 1278, 1363
RsaNI GTAC 5 cut(s) 95, 146, 1196, 1277, 1362
RseI CAYNNNNRTG 3 cut(s) 190, 1250, 1262
Rsr2I CGGWCCG 1 cut(s) 431
RsrII CGGWCCG 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 1666
SaqAI TTAA 4 cut(s) 758, 798, 1049, 1551
SatI GCNGC 5 cut(s) 317, 363, 453, 848, 1010
Sau3AI GATC 6 cut(s) 227, 355, 596, 751, 924, 1405
Sau96I GGNCC 2 cut(s) 257, 431
ScaI AGTACT 1 cut(s) 147
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 2 cut(s) 722, 1022
SduI GDGCHC 3 cut(s) 1186, 1257, 1418
SfaNI GCATC 2 cut(s) 619, 1411
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 1 cut(s) 431
SmiMI CAYNNNNRTG 3 cut(s) 190, 1250, 1262
SmlI CTYRAG 1 cut(s) 1550
SmoI CTYRAG 1 cut(s) 1550
Sse9I AATT 8 cut(s) 12, 71, 249, 467, 500, 621, 837, 945
SseBI AGGCCT 1 cut(s) 214
SsiI CCGC 2 cut(s) 113, 1292
SspI AATATT 2 cut(s) 828, 1345
SspMI CTAG 1 cut(s) 1154
StuI AGGCCT 1 cut(s) 214
StyD4I CCNGG 2 cut(s) 720, 1020
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 3 cut(s) 59, 150, 431
TaqI TCGA 4 cut(s) 253, 590, 785, 1240
TasI AATT 8 cut(s) 12, 71, 249, 467, 500, 621, 837, 945
TatI WGTACW 1 cut(s) 145
TfiI GAWTC 4 cut(s) 674, 950, 1267, 1570
Tru1I TTAA 4 cut(s) 758, 798, 1049, 1551
Tru9I TTAA 4 cut(s) 758, 798, 1049, 1551
TscAI CASTG 3 cut(s) 129, 988, 1126
TseFI GTSAC 3 cut(s) 124, 561, 1043
TseI GCWGC 5 cut(s) 316, 362, 452, 847, 1009
Tsp45I GTSAC 3 cut(s) 124, 561, 1043
TspDTI ATGAA 8 cut(s) 24, 174, 311, 366, 438, 674, 723, 1262
TspGWI ACGGA 1 cut(s) 631
TspRI CASTG 3 cut(s) 129, 988, 1126
Vha464I CTTAAG 1 cut(s) 1550
VpaK11BI GGWCC 1 cut(s) 431
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 3 cut(s) 12, 249, 500
XceI RCATGY 1 cut(s) 1495
XmnI GAANNNNTTC 2 cut(s) 395, 535
XspI CTAG 1 cut(s) 1154
ZrmI AGTACT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.