RchiOBHm_Chr1g0345031

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001937
Physical Location & Seq
Forward (+)
37413060 .. 37419835
6776 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57142

Sequence Viewer

Length: 1587 bp
ATGGAAGCAGAGTTAGCAGTAGCTGCAAAGATGGTAGTACTGTCAGTATTGGTATGTGGGCTTTTGTTCTTGGTTTCATATTTGTGTGAACTTCTGCTGTTGAGGCCTAAATCCATAAGATCAAAGCTGGAAAAGCAAGGAATTCGAGGGCCGCGTCCTACCTTTCTATTGGGAAACCTCCCTGAACAGAAGAGGCTCCAAAGCAAGCAGCAGGGTCAGTCAATAGCAACAAGGACTGATGATGATGATGATCATGCAGCTTCTGTTTCTCATCAATGGTATTTGAAAATCTTCCCCCACATAGAGCAATGGAGAAATGAATACGGTCCAATATTTTTGTGTTCATCTGCACACATACAACATTTAGTAATAACAGATATGGAAGTGGTGAAGGAAATTAGCTTGTGTAAGTCTCTAGTCTTGGGAAAACCTTCCGCAACATCCAAGGTTCTTGGGTCACTATTGGGAGAGGGAATTTTAGCGTCGAACGGATCAATGTGGGAGCATCAAAGGAAAATTATCGCTCCTGAACTATACCTTGATAAGGTGAAGGGCATGATGAACCTGTTAGTGGATTCTACAACCTCAATGCTAAGAACTTGGGAAAATGAAGTCCAAAGCCAGGGAGAACTTGCTGATATTAGAGTTGATGATCATTTGAGAAACTTATCAGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGATGCAAGAAGTTTTGGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACCGAAAATAGAAAGATATGGATGTTGGAAAAGGAAACCCAATCACTGATATTGAAGGTGGTAAAACAACGTAATGAAGGTTCATATGAGAAAGATTTGCTACAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGACTTAAATGGCCTATCACACAATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCACTGCCCTTGTAACATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTACTTGAAATCTGTGGTGATAAGCCTCTAGATGCAGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGCACTCTTTCTATCAAGAGAGGTATTTGAAACTATCAAATTTAAAAACATTACATTTCCAAAGGGGACGCTACTTCAAATACCAATACCCTTCTTACACCAGAACCCTGATCTGTGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTGCCAATGGAATTGTCAAGGCTTGCAAGTCTCCACAGGCCTACATGCCATTCGGAGCAGGTTCTCGTATATGTGTTGGTCAACACTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATGATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGCATACCAGCATTCTCCTGCATATAACATTATTCTGGAGCCTGAATATGGTATTATTCTACAAATGCGAAGAGCGTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

528

Amino Acids

59.67

Weight (kDa)

8.64

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 99 - 510 3.4e-83 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1397
AccB1I GGYRCC 1 cut(s) 929
AccII CGCG 1 cut(s) 154
AciI CCGC 3 cut(s) 152, 435, 1193
AclWI GGATC 2 cut(s) 499, 1324
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 3 cut(s) 141, 474, 1235
AfaI GTAC 2 cut(s) 39, 1098
AfiI CCNNNNNNNGG 4 cut(s) 544, 571, 622, 1550
AflII CTTAAG 1 cut(s) 1451
AgsI TTSAA 7 cut(s) 286, 865, 926, 1103, 1226, 1274, 1336
AjnI CCWGG 1 cut(s) 621
AleI CACNNNNGTG 1 cut(s) 1163
AluBI AGCT 9 cut(s) 23, 127, 260, 402, 692, 1044, 1058, 1079, 1091
AluI AGCT 9 cut(s) 23, 127, 260, 402, 692, 1044, 1058, 1079, 1091
Alw21I GWGCWC 2 cut(s) 1087, 1158
Alw26I GTCTC 3 cut(s) 417, 1010, 1383
AlwI GGATC 2 cut(s) 499, 1324
AlwNI CAGNNNCTG 2 cut(s) 23, 263
Ama87I CYCGRG 1 cut(s) 1086
AoxI GGCC 5 cut(s) 104, 149, 955, 1009, 1386
ApeKI GCWGC 3 cut(s) 23, 208, 257
ApoI RAATTY 3 cut(s) 141, 474, 1235
Asp700I GAANNNNTTC 2 cut(s) 290, 430
AspS9I GGNCC 2 cut(s) 149, 326
AsuHPI GGTGA 5 cut(s) 400, 559, 956, 1124, 1177
AvaI CYCGRG 1 cut(s) 1086
AvaII GGWCC 1 cut(s) 326
BalI TGGCCA 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 929
BauI CACGAG 1 cut(s) 1080
Bbv12I GWGCWC 2 cut(s) 1087, 1158
BbvCI CCTCAGC 1 cut(s) 1092
BbvI GCAGC 3 cut(s) 10, 220, 269
BccI CCATC 4 cut(s) 25, 769, 792, 1071
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 1 cut(s) 623
BclI TGATCA 2 cut(s) 250, 652
BcoDI GTCTC 3 cut(s) 417, 1010, 1383
BfaI CTAG 3 cut(s) 416, 1055, 1124
BfrI CTTAAG 1 cut(s) 1451
BfuAI ACCTGC 1 cut(s) 1397
BisI GCNGC 4 cut(s) 24, 152, 209, 258
BlsI GCNGC 4 cut(s) 25, 153, 210, 259
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 326
BmeT110I CYCGRG 1 cut(s) 1086
BmgT120I GGNCC 2 cut(s) 149, 326
BmiI GGNNCC 3 cut(s) 197, 931, 1542
BmrFI CCNGG 1 cut(s) 623
BmsI GCATC 4 cut(s) 514, 738, 1117, 1312
BpmI CTGGAG 1 cut(s) 1559
Bpu10I CCTNAGC 1 cut(s) 1092
BsaBI GATNNNNATC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 1010
BsaJI CCNNGG 2 cut(s) 444, 622
BsaXI ACNNNNNCTCC 2 cut(s) 1044, 1074
Bsc4I CCNNNNNNNGG 4 cut(s) 544, 571, 622, 1550
Bse3DI GCAATG 1 cut(s) 314
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 2 cut(s) 444, 622
BseGI GGATG 3 cut(s) 440, 837, 1063
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 4 cut(s) 544, 571, 622, 1550
BseMI GCAATG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 1083
BseXI GCAGC 3 cut(s) 10, 220, 269
BsgI GTGCAG 2 cut(s) 333, 1182
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 106, 151, 957, 1011, 1388
BshNI GGYRCC 1 cut(s) 929
BsiHKAI GWGCWC 2 cut(s) 1087, 1158
BsiHKCI CYCGRG 1 cut(s) 1086
BslFI GGGAC 1 cut(s) 1276
BslI CCNNNNNNNGG 4 cut(s) 544, 571, 622, 1550
BsmAI GTCTC 3 cut(s) 417, 1010, 1383
BsmFI GGGAC 1 cut(s) 1276
BsmI GAATGC 1 cut(s) 1513
BsnI GGCC 5 cut(s) 106, 151, 957, 1011, 1388
Bso31I GGTCTC 1 cut(s) 1010
BsoBI CYCGRG 1 cut(s) 1086
Bsp1286I GDGCHC 2 cut(s) 1087, 1158
Bsp143I GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
BspACI CCGC 3 cut(s) 152, 435, 1193
BspANI GGCC 5 cut(s) 106, 151, 957, 1011, 1388
BspCNI CTCAG 1 cut(s) 1084
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 197, 931, 1542
BspMI ACCTGC 1 cut(s) 1397
BspPI GGATC 2 cut(s) 499, 1324
BspQI GCTCTTC 1 cut(s) 1567
BspT107I GGYRCC 1 cut(s) 929
BspTI CTTAAG 1 cut(s) 1451
BspTNI GGTCTC 1 cut(s) 1010
BsrDI GCAATG 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 444, 622
BssMI GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
BssSI CACGAG 1 cut(s) 1080
BssT1I CCWWGG 1 cut(s) 444
Bst2BI CACGAG 1 cut(s) 1080
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 2 cut(s) 42, 326
Bst6I CTCTTC 2 cut(s) 185, 1567
BstAFI CTTAAG 1 cut(s) 1451
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 6 cut(s) 206, 1009, 1046, 1077, 1197, 1372
BstDEI CTNAG 2 cut(s) 593, 1092
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 3 cut(s) 440, 837, 1063
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 6 cut(s) 122, 253, 494, 655, 1309, 1319
BstMAI GTCTC 3 cut(s) 417, 1010, 1383
BstMBI GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
BstMWI GCNNNNNNNGC 5 cut(s) 14, 23, 103, 133, 916
BstNI CCWGG 1 cut(s) 623
BstNSI RCATGY 1 cut(s) 1396
BstSCI CCNGG 1 cut(s) 621
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 3 cut(s) 10, 220, 269
BstX2I RGATCY 1 cut(s) 1316
BstYI RGATCY 1 cut(s) 1316
BsuRI GGCC 5 cut(s) 106, 151, 957, 1011, 1388
BtsCI GGATG 3 cut(s) 440, 837, 1063
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 2 cut(s) 854, 1020
BveI ACCTGC 1 cut(s) 1397
Cac8I GCNNGC 6 cut(s) 206, 1009, 1046, 1077, 1197, 1372
CaiI CAGNNNCTG 2 cut(s) 23, 263
Cfr13I GGNCC 2 cut(s) 149, 326
CseI GACGC 5 cut(s) 143, 471, 1172, 1273, 1566
Csp6I GTAC 2 cut(s) 38, 1097
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviAII CATG 5 cut(s) 254, 556, 1013, 1038, 1393
CviQI GTAC 2 cut(s) 38, 1097
DdeI CTNAG 2 cut(s) 593, 1092
DpnI GATC 6 cut(s) 121, 252, 493, 654, 1308, 1318
DpnII GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
DraI TTTAAA 1 cut(s) 1240
EaeI YGGCCR 1 cut(s) 1009
Eam1104I CTCTTC 2 cut(s) 185, 1567
EarI CTCTTC 2 cut(s) 185, 1567
Eco130I CCWWGG 1 cut(s) 444
Eco147I AGGCCT 2 cut(s) 106, 1388
Eco31I GGTCTC 1 cut(s) 1010
Eco47I GGWCC 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 1086
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 621
EcoT14I CCWWGG 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 5 cut(s) 257, 559, 1016, 1041, 1396
FalI AAGNNNNNCTT 2 cut(s) 1472, 1504
FaqI GGGAC 1 cut(s) 1276
FatI CATG 5 cut(s) 253, 555, 1012, 1037, 1392
FauI CCCGC 1 cut(s) 1200
FauNDI CATATG 1 cut(s) 895
FbaI TGATCA 2 cut(s) 250, 652
Fnu4HI GCNGC 4 cut(s) 24, 152, 209, 258
FokI GGATG 3 cut(s) 427, 844, 1050
Fsp4HI GCNGC 4 cut(s) 24, 152, 209, 258
FspBI CTAG 3 cut(s) 416, 1055, 1124
GluI GCNGC 4 cut(s) 24, 152, 209, 258
GsuI CTGGAG 1 cut(s) 1559
HaeIII GGCC 5 cut(s) 106, 151, 957, 1011, 1388
HgaI GACGC 5 cut(s) 143, 471, 1172, 1273, 1566
Hin1II CATG 5 cut(s) 257, 559, 1016, 1041, 1396
HincII GTYRAC 1 cut(s) 1430
HindII GTYRAC 1 cut(s) 1430
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 3 cut(s) 575, 1168, 1471
HphI GGTGA 5 cut(s) 400, 559, 956, 1124, 1177
Hpy166II GTNNAC 2 cut(s) 89, 1430
Hpy188I TCNGA 3 cut(s) 807, 1321, 1403
Hpy188III TCNNGA 7 cut(s) 527, 923, 1067, 1124, 1172, 1212, 1538
Hpy8I GTNNAC 2 cut(s) 89, 1430
Hpy99I CGWCG 1 cut(s) 487
HpyAV CCTTC 7 cut(s) 385, 441, 544, 859, 881, 920, 1297
HpyCH4III ACNGT 2 cut(s) 42, 326
HpyCH4IV ACGT 1 cut(s) 880
HpyCH4V TGCA 9 cut(s) 26, 257, 350, 751, 990, 1130, 1199, 1374, 1523
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 23, 103, 133, 916
HpyF3I CTNAG 2 cut(s) 593, 1092
HpySE526I ACGT 1 cut(s) 880
Hsp92II CATG 5 cut(s) 257, 559, 1016, 1041, 1396
Ksp22I TGATCA 2 cut(s) 250, 652
Kzo9I GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
LguI GCTCTTC 1 cut(s) 1567
LmnI GCTCC 6 cut(s) 201, 502, 529, 1041, 1403, 1540
Lsp1109I GCAGC 3 cut(s) 10, 220, 269
LweI GCATC 4 cut(s) 514, 738, 1117, 1312
MaeI CTAG 3 cut(s) 416, 1055, 1124
MaeII ACGT 1 cut(s) 880
MaeIII GTNAC 4 cut(s) 456, 944, 1030, 1050
MalI GATC 6 cut(s) 121, 252, 493, 654, 1308, 1318
MboI GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
MboII GAAGA 4 cut(s) 202, 283, 757, 1584
MflI RGATCY 1 cut(s) 1316
MhlI GDGCHC 2 cut(s) 1087, 1158
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 8 cut(s) 141, 396, 474, 516, 706, 738, 1235, 1359
MluNI TGGCCA 1 cut(s) 1011
MmeI TCCRAC 2 cut(s) 816, 830
Mox20I TGGCCA 1 cut(s) 1011
MroXI GAANNNNTTC 2 cut(s) 290, 430
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 3 cut(s) 950, 1239, 1452
MslI CAYNNNNRTG 2 cut(s) 82, 1163
Msp20I TGGCCA 1 cut(s) 1011
MspCI CTTAAG 1 cut(s) 1451
MspR9I CCNGG 1 cut(s) 623
Mva1269I GAATGC 1 cut(s) 1513
MvaI CCWGG 1 cut(s) 623
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 5 cut(s) 14, 23, 103, 133, 916
NdeI CATATG 1 cut(s) 895
NdeII GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
NlaIII CATG 5 cut(s) 257, 559, 1016, 1041, 1396
NlaIV GGNNCC 3 cut(s) 197, 931, 1542
NmuCI GTSAC 2 cut(s) 456, 944
NspI RCATGY 1 cut(s) 1396
OliI CACNNNNGTG 1 cut(s) 1163
PaeR7I CTCGAG 1 cut(s) 1086
PceI AGGCCT 2 cut(s) 106, 1388
PciSI GCTCTTC 1 cut(s) 1567
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PctI GAATGC 1 cut(s) 1513
PdmI GAANNNNTTC 2 cut(s) 290, 430
PfeI GAWTC 3 cut(s) 575, 1168, 1471
PkrI GCNGC 4 cut(s) 25, 153, 210, 259
Psp6I CCWGG 1 cut(s) 621
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 3 cut(s) 197, 931, 1542
PspPI GGNCC 2 cut(s) 149, 326
PspXI VCTCGAGB 1 cut(s) 1086
PsrI GAACNNNNNNTAC 2 cut(s) 874, 906
PstNI CAGNNNCTG 2 cut(s) 23, 263
PsuI RGATCY 1 cut(s) 1316
RsaI GTAC 2 cut(s) 39, 1098
RsaNI GTAC 2 cut(s) 38, 1097
RseI CAYNNNNRTG 2 cut(s) 82, 1163
SapI GCTCTTC 1 cut(s) 1567
SaqAI TTAA 3 cut(s) 950, 1239, 1452
SatI GCNGC 4 cut(s) 24, 152, 209, 258
Sau3AI GATC 6 cut(s) 119, 250, 491, 652, 1306, 1316
Sau96I GGNCC 2 cut(s) 149, 326
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 623
SduI GDGCHC 2 cut(s) 1087, 1158
SfaNI GCATC 4 cut(s) 514, 738, 1117, 1312
Sfr274I CTCGAG 1 cut(s) 1086
SinI GGWCC 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 1086
SmiMI CAYNNNNRTG 2 cut(s) 82, 1163
SmlI CTYRAG 2 cut(s) 1086, 1451
SmoI CTYRAG 2 cut(s) 1086, 1451
Sse9I AATT 8 cut(s) 141, 396, 474, 516, 706, 738, 1235, 1359
SseBI AGGCCT 2 cut(s) 106, 1388
SsiI CCGC 3 cut(s) 152, 435, 1193
SspI AATATT 2 cut(s) 333, 729
SspMI CTAG 3 cut(s) 416, 1055, 1124
StuI AGGCCT 2 cut(s) 106, 1388
StyD4I CCNGG 1 cut(s) 621
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 2 cut(s) 42, 326
TaiI ACGT 1 cut(s) 883
TaqI TCGA 4 cut(s) 145, 485, 1087, 1141
TasI AATT 8 cut(s) 141, 396, 474, 516, 706, 738, 1235, 1359
TatI WGTACW 1 cut(s) 37
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 3 cut(s) 575, 1168, 1471
Tru1I TTAA 3 cut(s) 950, 1239, 1452
Tru9I TTAA 3 cut(s) 950, 1239, 1452
TscAI CASTG 2 cut(s) 861, 1027
TseFI GTSAC 2 cut(s) 456, 944
TseI GCWGC 3 cut(s) 23, 208, 257
Tsp45I GTSAC 2 cut(s) 456, 944
TspDTI ATGAA 9 cut(s) 66, 333, 333, 575, 624, 689, 882, 900, 1163
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 2 cut(s) 861, 1027
Vha464I CTTAAG 1 cut(s) 1451
VpaK11BI GGWCC 1 cut(s) 326
XagI CCTNNNNNAGG 1 cut(s) 542
XapI RAATTY 3 cut(s) 141, 474, 1235
XbaI TCTAGA 1 cut(s) 1123
XceI RCATGY 1 cut(s) 1396
XhoI CTCGAG 1 cut(s) 1086
XmnI GAANNNNTTC 2 cut(s) 290, 430
XspI CTAG 3 cut(s) 416, 1055, 1124
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.