RLG00000028859

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001937
Physical Location & Seq
Reverse (-)
29437105 .. 29441089
3985 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028859

Sequence Viewer

Length: 1410 bp
ATGGAAGCAGAGTTAGCAGTAGCTGCAAAGATGGTAGTACTGTCAGTATTGGTGTGTGGGCTTTTGTTCTTGGTTTCATATTTGTGTGAACTTCTGCTGTTGAGGCCTAAATCCATAAGATCAAAGCTGGAAAAGCAAGGAATTCGAGGGCCTCGTCCTACCTTTCTATTGGGAAACCTCCCTGAACAGAAGAGGCTCCAAACCAAGCAGCAGGGTCAGTCAATAGCAGCAAGGACTGATGATGATGATGATGATCATGCAACTTCTGTTTCTCATCAATGGTATTTGAAAATCTTCCCCCACATAGAGCAATGGAGAAATGAATACGGTCCATTATTTTTGTGTTCATCTGCACACATACAACAATTAGTGAAGGGCATGATGAACCTGGTAGTCGATTCTACAACCACAATGCTAAGAACTTGGGAAAATGAAGTCCACAGGCAGGGAGAACTTGCTGAAATTAGAGTTGATGATCATTTGAGAAACTTATCAGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGCTGCAAGAAGTTTTGGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACAGAAAATATAAAGATATGGATGTTGGAAAAGGAAACTCAATCACTGATATTGAAGGTGGTAAAACAACGTAATGAAGGTTCATATGAGAAAGATTTGCTGCAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGATTTAAATGGCCTATCACACAATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCACTGCCCTTGTAGCATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTACTTGAAATCTGTGGTGATAAGCCTCTAGATGCTGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGTACTCTTTGTAGCAAGAGAGGTATTTGAAACTATCAAATTTAAAAACATTACAATTCCAAAGGGGACGCTACTTCAAATACCAATCCCCTTCTTACACCAGAACCCTGATCTATGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTACCAATGGAATTGTCAAGGCTTGCAAGTCTCCACAGGCCTACATGCCATTCGGAGCAGGTTCTCGTATATGTGTTGGTCAACACTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATAATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGTATACCAGCATTCTCCTGCGTATAACATTATTCTGGAGCCTGAATATGGTATTATTCTACAAATGCGAAGAGCATCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

470

Amino Acids

53.32

Weight (kDa)

8.59

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 124 - 448 7e-70 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1220
AccB1I GGYRCC 1 cut(s) 752
AccI GTMKAC 1 cut(s) 1329
AciI CCGC 1 cut(s) 1016
AclWI GGATC 1 cut(s) 1147
AcoI YGGCCR 1 cut(s) 832
AcsI RAATTY 2 cut(s) 141, 1058
AfaI GTAC 3 cut(s) 39, 921, 1023
AfiI CCNNNNNNNGG 2 cut(s) 445, 1373
AflII CTTAAG 1 cut(s) 1274
AgsI TTSAA 7 cut(s) 289, 688, 749, 926, 1049, 1097, 1159
AjnI CCWGG 1 cut(s) 387
AleI CACNNNNGTG 1 cut(s) 986
AluBI AGCT 8 cut(s) 23, 127, 515, 571, 867, 881, 902, 914
AluI AGCT 8 cut(s) 23, 127, 515, 571, 867, 881, 902, 914
Alw21I GWGCWC 2 cut(s) 910, 981
Alw26I GTCTC 2 cut(s) 833, 1206
AlwI GGATC 1 cut(s) 1147
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 909
AoxI GGCC 5 cut(s) 104, 149, 778, 832, 1209
ApeKI GCWGC 5 cut(s) 23, 208, 227, 571, 733
ApoI RAATTY 2 cut(s) 141, 1058
Asp700I GAANNNNTTC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 149, 329
AsuHPI GGTGA 3 cut(s) 779, 947, 1000
AvaI CYCGRG 1 cut(s) 909
AvaII GGWCC 1 cut(s) 329
BalI TGGCCA 1 cut(s) 834
BanI GGYRCC 1 cut(s) 752
BauI CACGAG 1 cut(s) 903
Bbv12I GWGCWC 2 cut(s) 910, 981
BbvCI CCTCAGC 1 cut(s) 915
BbvI GCAGC 5 cut(s) 10, 220, 239, 558, 720
BccI CCATC 4 cut(s) 25, 592, 615, 894
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 2 cut(s) 253, 475
BcoDI GTCTC 2 cut(s) 833, 1206
BfaI CTAG 2 cut(s) 878, 947
BfrI CTTAAG 1 cut(s) 1274
BfuAI ACCTGC 1 cut(s) 1220
BisI GCNGC 5 cut(s) 24, 209, 228, 572, 734
BlsI GCNGC 5 cut(s) 25, 210, 229, 573, 735
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 329
BmeT110I CYCGRG 1 cut(s) 909
BmgT120I GGNCC 2 cut(s) 149, 329
BmiI GGNNCC 3 cut(s) 197, 754, 1365
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 3 cut(s) 866, 940, 1135
BpmI CTGGAG 1 cut(s) 1382
Bpu10I CCTNAGC 1 cut(s) 915
BsaBI GATNNNNATC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 833
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 1373
Bse3DI GCAATG 1 cut(s) 317
Bse8I GATNNNNATC 1 cut(s) 252
BseBI CCWGG 1 cut(s) 389
BseGI GGATG 2 cut(s) 660, 886
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 445, 1373
BseMI GCAATG 1 cut(s) 317
BseMII CTCAG 1 cut(s) 906
BseXI GCAGC 5 cut(s) 10, 220, 239, 558, 720
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 5 cut(s) 106, 151, 780, 834, 1211
BshNI GGYRCC 1 cut(s) 752
BsiHKAI GWGCWC 2 cut(s) 910, 981
BsiHKCI CYCGRG 1 cut(s) 909
BslFI GGGAC 1 cut(s) 1099
BslI CCNNNNNNNGG 2 cut(s) 445, 1373
BsmAI GTCTC 2 cut(s) 833, 1206
BsmFI GGGAC 1 cut(s) 1099
BsmI GAATGC 1 cut(s) 1336
BsnI GGCC 5 cut(s) 106, 151, 780, 834, 1211
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 1 cut(s) 909
Bsp1286I GDGCHC 2 cut(s) 910, 981
Bsp143I GATC 5 cut(s) 119, 253, 475, 1129, 1139
BspACI CCGC 1 cut(s) 1016
BspANI GGCC 5 cut(s) 106, 151, 780, 834, 1211
BspCNI CTCAG 1 cut(s) 907
BspLI GGNNCC 3 cut(s) 197, 754, 1365
BspMI ACCTGC 1 cut(s) 1220
BspPI GGATC 1 cut(s) 1147
BspQI GCTCTTC 1 cut(s) 1390
BspT107I GGYRCC 1 cut(s) 752
BspTI CTTAAG 1 cut(s) 1274
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 1 cut(s) 317
BssMI GATC 5 cut(s) 119, 253, 475, 1129, 1139
BssNAI GTATAC 1 cut(s) 1330
BssSI CACGAG 1 cut(s) 903
Bst1107I GTATAC 1 cut(s) 1330
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 2 cut(s) 42, 329
Bst6I CTCTTC 2 cut(s) 185, 1390
BstAFI CTTAAG 1 cut(s) 1274
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 4 cut(s) 832, 869, 900, 1195
BstDEI CTNAG 2 cut(s) 416, 915
BstF5I GGATG 2 cut(s) 660, 886
BstKTI GATC 5 cut(s) 122, 256, 478, 1132, 1142
BstMAI GTCTC 2 cut(s) 833, 1206
BstMBI GATC 5 cut(s) 119, 253, 475, 1129, 1139
BstMWI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 739, 854
BstNI CCWGG 1 cut(s) 389
BstNSI RCATGY 1 cut(s) 1219
BstSCI CCNGG 1 cut(s) 387
BstV1I GCAGC 5 cut(s) 10, 220, 239, 558, 720
BstX2I RGATCY 1 cut(s) 1139
BstYI RGATCY 1 cut(s) 1139
BstZ17I GTATAC 1 cut(s) 1330
BsuRI GGCC 5 cut(s) 106, 151, 780, 834, 1211
BtsCI GGATG 2 cut(s) 660, 886
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 2 cut(s) 677, 843
BveI ACCTGC 1 cut(s) 1220
Cac8I GCNNGC 4 cut(s) 832, 869, 900, 1195
CaiI CAGNNNCTG 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 149, 329
CseI GACGC 2 cut(s) 995, 1096
CsiI ACCWGGT 1 cut(s) 387
Csp6I GTAC 3 cut(s) 38, 920, 1022
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 5 cut(s) 257, 379, 836, 861, 1216
CviQI GTAC 3 cut(s) 38, 920, 1022
DdeI CTNAG 2 cut(s) 416, 915
DpnI GATC 5 cut(s) 121, 255, 477, 1131, 1141
DpnII GATC 5 cut(s) 119, 253, 475, 1129, 1139
DraI TTTAAA 2 cut(s) 774, 1063
EaeI YGGCCR 1 cut(s) 832
Eam1104I CTCTTC 2 cut(s) 185, 1390
EarI CTCTTC 2 cut(s) 185, 1390
Eco147I AGGCCT 2 cut(s) 106, 1211
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 909
EcoO109I RGGNCCY 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 5 cut(s) 260, 382, 839, 864, 1219
FalI AAGNNNNNCTT 2 cut(s) 1295, 1327
FaqI GGGAC 1 cut(s) 1099
FatI CATG 5 cut(s) 256, 378, 835, 860, 1215
FauI CCCGC 1 cut(s) 1023
FauNDI CATATG 1 cut(s) 718
FbaI TGATCA 2 cut(s) 253, 475
FblI GTMKAC 1 cut(s) 1329
Fnu4HI GCNGC 5 cut(s) 24, 209, 228, 572, 734
FokI GGATG 2 cut(s) 667, 873
Fsp4HI GCNGC 5 cut(s) 24, 209, 228, 572, 734
FspBI CTAG 2 cut(s) 878, 947
GluI GCNGC 5 cut(s) 24, 209, 228, 572, 734
GsuI CTGGAG 1 cut(s) 1382
HaeIII GGCC 5 cut(s) 106, 151, 780, 834, 1211
HgaI GACGC 2 cut(s) 995, 1096
Hin1II CATG 5 cut(s) 260, 382, 839, 864, 1219
HincII GTYRAC 1 cut(s) 1253
HindII GTYRAC 1 cut(s) 1253
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 2 cut(s) 398, 991
HphI GGTGA 3 cut(s) 779, 947, 1000
Hpy166II GTNNAC 4 cut(s) 89, 439, 1253, 1330
Hpy188I TCNGA 3 cut(s) 630, 1144, 1226
Hpy188III TCNNGA 5 cut(s) 746, 890, 947, 995, 1361
Hpy8I GTNNAC 4 cut(s) 89, 439, 1253, 1330
HpyAV CCTTC 5 cut(s) 367, 682, 704, 743, 1120
HpyCH4III ACNGT 2 cut(s) 42, 329
HpyCH4IV ACGT 1 cut(s) 703
HpyCH4V TGCA 7 cut(s) 26, 260, 353, 574, 736, 813, 1197
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 739, 854
HpyF3I CTNAG 2 cut(s) 416, 915
HpySE526I ACGT 1 cut(s) 703
Hsp92II CATG 5 cut(s) 260, 382, 839, 864, 1219
Ksp22I TGATCA 2 cut(s) 253, 475
Kzo9I GATC 5 cut(s) 119, 253, 475, 1129, 1139
LguI GCTCTTC 1 cut(s) 1390
LmnI GCTCC 4 cut(s) 201, 864, 1226, 1363
Lsp1109I GCAGC 5 cut(s) 10, 220, 239, 558, 720
LweI GCATC 3 cut(s) 866, 940, 1135
MabI ACCWGGT 1 cut(s) 387
MaeI CTAG 2 cut(s) 878, 947
MaeII ACGT 1 cut(s) 703
MaeIII GTNAC 1 cut(s) 873
MalI GATC 5 cut(s) 121, 255, 477, 1131, 1141
MboI GATC 5 cut(s) 119, 253, 475, 1129, 1139
MboII GAAGA 3 cut(s) 202, 286, 1407
MflI RGATCY 1 cut(s) 1139
MhlI GDGCHC 2 cut(s) 910, 981
MlsI TGGCCA 1 cut(s) 834
MluCI AATT 9 cut(s) 141, 365, 462, 529, 561, 1058, 1074, 1182, 1293
MluNI TGGCCA 1 cut(s) 834
MmeI TCCRAC 2 cut(s) 639, 653
Mox20I TGGCCA 1 cut(s) 834
MroXI GAANNNNTTC 1 cut(s) 293
MscI TGGCCA 1 cut(s) 834
MseI TTAA 3 cut(s) 773, 1062, 1275
MslI CAYNNNNRTG 2 cut(s) 82, 986
Msp20I TGGCCA 1 cut(s) 834
MspCI CTTAAG 1 cut(s) 1274
MspR9I CCNGG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 1336
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 739, 854
NdeI CATATG 1 cut(s) 718
NdeII GATC 5 cut(s) 119, 253, 475, 1129, 1139
NlaIII CATG 5 cut(s) 260, 382, 839, 864, 1219
NlaIV GGNNCC 3 cut(s) 197, 754, 1365
NspI RCATGY 1 cut(s) 1219
OliI CACNNNNGTG 1 cut(s) 986
PaeR7I CTCGAG 1 cut(s) 909
PceI AGGCCT 2 cut(s) 106, 1211
PciSI GCTCTTC 1 cut(s) 1390
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PctI GAATGC 1 cut(s) 1336
PdmI GAANNNNTTC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 398, 991
PkrI GCNGC 5 cut(s) 25, 210, 229, 573, 735
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 3 cut(s) 197, 754, 1365
PspPI GGNCC 2 cut(s) 149, 329
PspXI VCTCGAGB 1 cut(s) 909
PsrI GAACNNNNNNTAC 2 cut(s) 697, 729
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 1139
RsaI GTAC 3 cut(s) 39, 921, 1023
RsaNI GTAC 3 cut(s) 38, 920, 1022
RseI CAYNNNNRTG 2 cut(s) 82, 986
SapI GCTCTTC 1 cut(s) 1390
SaqAI TTAA 3 cut(s) 773, 1062, 1275
SatI GCNGC 5 cut(s) 24, 209, 228, 572, 734
Sau3AI GATC 5 cut(s) 119, 253, 475, 1129, 1139
Sau96I GGNCC 2 cut(s) 149, 329
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 2 cut(s) 910, 981
SexAI ACCWGGT 1 cut(s) 387
SfaNI GCATC 3 cut(s) 866, 940, 1135
Sfr274I CTCGAG 1 cut(s) 909
SinI GGWCC 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 909
SmiI ATTTAAAT 1 cut(s) 774
SmiMI CAYNNNNRTG 2 cut(s) 82, 986
SmlI CTYRAG 2 cut(s) 909, 1274
SmoI CTYRAG 2 cut(s) 909, 1274
Sse9I AATT 9 cut(s) 141, 365, 462, 529, 561, 1058, 1074, 1182, 1293
SseBI AGGCCT 2 cut(s) 106, 1211
SsiI CCGC 1 cut(s) 1016
SspI AATATT 1 cut(s) 552
SspMI CTAG 2 cut(s) 878, 947
StuI AGGCCT 2 cut(s) 106, 1211
StyD4I CCNGG 1 cut(s) 387
SwaI ATTTAAAT 1 cut(s) 774
TaaI ACNGT 2 cut(s) 42, 329
TaiI ACGT 1 cut(s) 706
TaqI TCGA 4 cut(s) 145, 396, 910, 964
TasI AATT 9 cut(s) 141, 365, 462, 529, 561, 1058, 1074, 1182, 1293
TatI WGTACW 2 cut(s) 37, 1021
TfiI GAWTC 2 cut(s) 398, 991
Tru1I TTAA 3 cut(s) 773, 1062, 1275
Tru9I TTAA 3 cut(s) 773, 1062, 1275
TscAI CASTG 2 cut(s) 684, 850
TseI GCWGC 5 cut(s) 23, 208, 227, 571, 733
TspDTI ATGAA 9 cut(s) 66, 336, 336, 398, 447, 512, 705, 723, 986
TspRI CASTG 2 cut(s) 684, 850
Vha464I CTTAAG 1 cut(s) 1274
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 2 cut(s) 141, 1058
XbaI TCTAGA 1 cut(s) 946
XceI RCATGY 1 cut(s) 1219
XhoI CTCGAG 1 cut(s) 909
XmiI GTMKAC 1 cut(s) 1329
XmnI GAANNNNTTC 1 cut(s) 293
XspI CTAG 2 cut(s) 878, 947
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.