RLG00000028855

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001937
Physical Location & Seq
Reverse (-)
29330446 .. 29334211
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028855

Sequence Viewer

Length: 1566 bp
ATGAAGATTTTGAAGCAGCAGCAAATGGAAGGTGAGAAAATGGAAGCAGAGCTATTAGTGGTAGCAGCAAAGATCATATTGTCAGTATTGGTGGGTGGGTTTCTGCTGTTGTTCTTGTATCTCTATGAACTACTGATATTGAGGCCTAAAGGGCTAAGATCAAAGCTCCAAAAGCAAGGAATTAGAGGGCCTCCTCCTTCATTTCTCTTGGGGAATATCCTTGCATTAAAGAGCCTTCAAAGCAAGGTCCAGTCAACTGCATCAAAGACTACAGATCCTCAAAAGCCCTATGATGATCGTGTAGCTTCTATTTGTCATCAATGGCCTTCAAAAATCTTCCCCCACATAGAGGAATGGAGAAATGAATATGGGCCAACATTTATGTATGCACTTGTCCACATGCAACAATTATGTATAACAGATGTAGAGCTATTGAAGGAACTTGGGCTTTGCAAGTCATTAATCTTGGGCACTCCTTCCATAACGTCCAAGATTCTTGGACCTCTTCTGGGACAGGGTCTTTTATCATCGAATGGATTAATTTGGGCCCATCAAAGGAAAACTATCGCTCCTGAATTATACCTTGCTAAGATCAAGGGCATGATGGACCTAATAGTGGACTGTACAACCTCAGTGCTAAGAACTTGGGAAAGTGAAATCCAAACGCAGGGAGGAACTGCAGAAATTAGAGTTGATGAGAATCTAAGAAACATATCTGGAGATATAATTGCAAAAGCTTGTTTTCAGAGCAATTATAGTGAAGGAGAAGAAATATTCTCAAGACTTAGGACTCTACAAAAGGTTTTGTCAAAGGGTATGATGGGGGTAGAAGGCTTAATCAGATGGTTGCCAACAAACGAAAATAGAGAAATATGGAGATTAGAGAAGGAAATTGAGTCAACGATATTGAAGGTGGTAAAACAGCGCAGTGAAGCTTCATATGAGAAAGATTTGCTGCAAATGATTCTTCAGGGTGCTGAAACTTCGGGTGAAAATGGCCTATCACATAAGAAGTTCATTGTTGATAATTGCAAAACCATATTCATTGCTGCCCATGAGACCACTGCCATTGCAGCATCATGGAGCTTGATGCTACTAGCTTTACATCCAGATTGGCAAGCTCGTGTTCGTGCTGAGGTGCTTGAAATCTGCGGGGATAGGCCTCTACATGCAGATATGCTTCCAAGCATGAAAGTGCTGACAATGGTGATTCAGGAAGTATTGCGTCTATACTCGCCTGTATTTTTTTTAGCAAGAGAGGTCCTTGAAACTATCACATTGAAAAACATTGTGATACCAAAGGGTGTGGTCCTTCAGATACCAGTACCCTTTCTACACCAGAACCCTGATATATGGGGCCCTGACGCGCATAAGTTCAATCCTGAAAGATTTGCCAATGGAGTTCTCAAGGCTTGCAACTCTCCACATGCCTACATGCCATTCGGCCTTGGTCCCCGAATCTGTGTTGGTCAACATTTAGCAATAACAGAGCTTAAGGTGATTCTTTCTTTGATTCTATCAAAGTTTTGTTTTTCCCTCTCACCACCAGCATTCCCCTGCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

522

Amino Acids

58.47

Weight (kDa)

8.62

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 113 - 515 7.6e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1564
AccII CGCG 1 cut(s) 1367
AciI CCGC 1 cut(s) 1152
AclWI GGATC 1 cut(s) 269
AcuI CTGAAG 2 cut(s) 953, 1298
AfaI GTAC 2 cut(s) 625, 1326
AfiI CCNNNNNNNGG 5 cut(s) 349, 509, 555, 616, 667
AflII CTTAAG 1 cut(s) 1493
AgsI TTSAA 9 cut(s) 13, 239, 330, 436, 910, 1145, 1268, 1282, 1378
Alw26I GTCTC 1 cut(s) 1052
AlwI GGATC 1 cut(s) 269
AoxI GGCC 9 cut(s) 143, 188, 323, 371, 546, 997, 1160, 1357, 1444
ApaI GGGCCC 2 cut(s) 550, 1361
ApeKI GCWGC 6 cut(s) 16, 19, 65, 955, 1049, 1073
AseI ATTAAT 2 cut(s) 461, 539
AspLEI GCGC 2 cut(s) 927, 1369
AsuHPI GGTGA 5 cut(s) 44, 1001, 1219, 1510, 1533
AvaII GGWCC 6 cut(s) 247, 500, 607, 1261, 1309, 1451
BaeGI GKGCMC 3 cut(s) 473, 550, 1361
BanII GRGCYC 2 cut(s) 550, 1361
BauI CACGAG 1 cut(s) 1122
BbvCI CCTCAGC 1 cut(s) 1134
BbvI GCAGC 6 cut(s) 28, 31, 77, 942, 1036, 1085
BccI CCATC 4 cut(s) 558, 598, 814, 837
BcoDI GTCTC 1 cut(s) 1052
BfaI CTAG 1 cut(s) 1097
BfmI CTRYAG 2 cut(s) 270, 678
BfrI CTTAAG 1 cut(s) 1493
BglI GCCNNNNNGGC 1 cut(s) 151
BisI GCNGC 6 cut(s) 17, 20, 66, 956, 1050, 1074
BlsI GCNGC 6 cut(s) 18, 21, 67, 957, 1051, 1075
Bme18I GGWCC 6 cut(s) 247, 500, 607, 1261, 1309, 1451
BmiI GGNNCC 4 cut(s) 548, 1358, 1359, 1453
BmsI GCATC 3 cut(s) 269, 1080, 1085
BpmI CTGGAG 1 cut(s) 738
Bpu10I CCTNAGC 1 cut(s) 1134
BpuEI CTTGAG 2 cut(s) 763, 1391
BsaBI GATNNNNATC 1 cut(s) 699
BsaI GGTCTC 1 cut(s) 1052
BsaJI CCNNGG 1 cut(s) 1447
BsaXI ACNNNNNCTCC 2 cut(s) 553, 583
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 509, 555, 616, 667
Bse1I ACTGG 2 cut(s) 250, 1322
Bse3DI GCAATG 2 cut(s) 1044, 1068
Bse8I GATNNNNATC 1 cut(s) 699
BseDI CCNNGG 1 cut(s) 1447
BseGI GGATG 1 cut(s) 1105
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 5 cut(s) 349, 509, 555, 616, 667
BseMI GCAATG 2 cut(s) 1044, 1068
BseMII CTCAG 2 cut(s) 645, 1125
BseNI ACTGG 2 cut(s) 250, 1322
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 3 cut(s) 473, 550, 1361
BseXI GCAGC 6 cut(s) 28, 31, 77, 942, 1036, 1085
Bsh1236I CGCG 1 cut(s) 1367
BshFI GGCC 9 cut(s) 145, 190, 325, 373, 548, 999, 1162, 1359, 1446
BslFI GGGAC 2 cut(s) 525, 1437
BslI CCNNNNNNNGG 5 cut(s) 349, 509, 555, 616, 667
BsmAI GTCTC 1 cut(s) 1052
BsmFI GGGAC 2 cut(s) 525, 1437
BsmI GAATGC 1 cut(s) 1550
BsnI GGCC 9 cut(s) 145, 190, 325, 373, 548, 999, 1162, 1359, 1446
Bso31I GGTCTC 1 cut(s) 1052
Bsp120I GGGCCC 2 cut(s) 546, 1357
Bsp1286I GDGCHC 3 cut(s) 473, 550, 1361
Bsp1407I TGTACA 1 cut(s) 623
Bsp143I GATC 5 cut(s) 72, 158, 274, 295, 591
BspACI CCGC 1 cut(s) 1152
BspANI GGCC 9 cut(s) 145, 190, 325, 373, 548, 999, 1162, 1359, 1446
BspCNI CTCAG 2 cut(s) 644, 1126
BspFNI CGCG 1 cut(s) 1367
BspLI GGNNCC 4 cut(s) 548, 1358, 1359, 1453
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 1 cut(s) 269
BspTI CTTAAG 1 cut(s) 1493
BspTNI GGTCTC 1 cut(s) 1052
BsrDI GCAATG 2 cut(s) 1044, 1068
BsrGI TGTACA 1 cut(s) 623
BsrI ACTGG 2 cut(s) 250, 1322
BssECI CCNNGG 1 cut(s) 1447
BssMI GATC 5 cut(s) 72, 158, 274, 295, 591
BssSI CACGAG 1 cut(s) 1122
BssT1I CCWWGG 1 cut(s) 1447
Bst2BI CACGAG 1 cut(s) 1122
Bst4CI ACNGT 1 cut(s) 623
Bst6I CTCTTC 1 cut(s) 510
BstAFI CTTAAG 1 cut(s) 1493
BstAUI TGTACA 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 1119, 1414
BstDEI CTNAG 7 cut(s) 155, 588, 631, 638, 704, 785, 1134
BstF5I GGATG 1 cut(s) 1105
BstFNI CGCG 1 cut(s) 1367
BstHHI GCGC 2 cut(s) 927, 1369
BstKTI GATC 5 cut(s) 75, 161, 277, 298, 594
BstMAI GTCTC 1 cut(s) 1052
BstMBI GATC 5 cut(s) 72, 158, 274, 295, 591
BstMWI GCNNNNNNNGC 4 cut(s) 151, 172, 240, 1073
BstNSI RCATGY 4 cut(s) 403, 1172, 1430, 1438
BstSFI CTRYAG 2 cut(s) 270, 678
BstSLI GKGCMC 3 cut(s) 473, 550, 1361
BstUI CGCG 1 cut(s) 1367
BstV1I GCAGC 6 cut(s) 28, 31, 77, 942, 1036, 1085
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 9 cut(s) 145, 190, 325, 373, 548, 999, 1162, 1359, 1446
BtsCI GGATG 1 cut(s) 1105
BtsI GCAGTG 2 cut(s) 934, 1062
BtsIMutI CAGTG 3 cut(s) 639, 934, 1062
Cac8I GCNNGC 2 cut(s) 1119, 1414
CfoI GCGC 2 cut(s) 927, 1369
CseI GACGC 2 cut(s) 1214, 1373
Csp6I GTAC 2 cut(s) 624, 1325
CspCI CAANNNNNGTGG 4 cut(s) 1051, 1086, 1287, 1322
CviAII CATG 8 cut(s) 400, 601, 1055, 1080, 1169, 1189, 1427, 1435
CviQI GTAC 2 cut(s) 624, 1325
DdeI CTNAG 7 cut(s) 155, 588, 631, 638, 704, 785, 1134
DpnI GATC 5 cut(s) 74, 160, 276, 297, 593
DpnII GATC 5 cut(s) 72, 158, 274, 295, 591
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 1447
Eco147I AGGCCT 2 cut(s) 145, 1162
Eco24I GRGCYC 2 cut(s) 550, 1361
Eco31I GGTCTC 1 cut(s) 1052
Eco47I GGWCC 6 cut(s) 247, 500, 607, 1261, 1309, 1451
Eco57I CTGAAG 2 cut(s) 953, 1298
EcoO109I RGGNCCY 4 cut(s) 188, 1261, 1357, 1358
EcoT14I CCWWGG 1 cut(s) 1447
EcoT38I GRGCYC 2 cut(s) 550, 1361
ErhI CCWWGG 1 cut(s) 1447
FaeI CATG 8 cut(s) 403, 604, 1058, 1083, 1172, 1192, 1430, 1438
FaqI GGGAC 2 cut(s) 525, 1437
FatI CATG 8 cut(s) 399, 600, 1054, 1079, 1168, 1188, 1426, 1434
FauI CCCGC 1 cut(s) 1145
FauNDI CATATG 1 cut(s) 940
Fnu4HI GCNGC 6 cut(s) 17, 20, 66, 956, 1050, 1074
FokI GGATG 1 cut(s) 1092
FriOI GRGCYC 2 cut(s) 550, 1361
Fsp4HI GCNGC 6 cut(s) 17, 20, 66, 956, 1050, 1074
FspBI CTAG 1 cut(s) 1097
GlaI GCGC 2 cut(s) 926, 1368
GluI GCNGC 6 cut(s) 17, 20, 66, 956, 1050, 1074
GsuI CTGGAG 1 cut(s) 738
HaeIII GGCC 9 cut(s) 145, 190, 325, 373, 548, 999, 1162, 1359, 1446
HgaI GACGC 2 cut(s) 1214, 1373
HhaI GCGC 2 cut(s) 927, 1369
Hin1II CATG 8 cut(s) 403, 604, 1058, 1083, 1172, 1192, 1430, 1438
Hin6I GCGC 2 cut(s) 925, 1367
HinP1I GCGC 2 cut(s) 925, 1367
HincII GTYRAC 3 cut(s) 255, 900, 1472
HindII GTYRAC 3 cut(s) 255, 900, 1472
HindIII AAGCTT 2 cut(s) 735, 933
HinfI GANTC 9 cut(s) 493, 700, 790, 896, 964, 1210, 1458, 1501, 1513
HphI GGTGA 5 cut(s) 44, 1001, 1219, 1510, 1533
Hpy166II GTNNAC 5 cut(s) 255, 397, 619, 900, 1472
Hpy188I TCNGA 3 cut(s) 747, 842, 1317
Hpy188III TCNNGA 6 cut(s) 572, 717, 780, 1109, 1214, 1382
Hpy8I GTNNAC 5 cut(s) 255, 397, 619, 900, 1472
HpyCH4III ACNGT 1 cut(s) 623
HpyCH4IV ACGT 1 cut(s) 485
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 172, 240, 1073
HpyF3I CTNAG 7 cut(s) 155, 588, 631, 638, 704, 785, 1134
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 8 cut(s) 403, 604, 1058, 1083, 1172, 1192, 1430, 1438
HspAI GCGC 2 cut(s) 925, 1367
Kzo9I GATC 5 cut(s) 72, 158, 274, 295, 591
LmnI GCTCC 3 cut(s) 171, 574, 1083
Lsp1109I GCAGC 6 cut(s) 28, 31, 77, 942, 1036, 1085
LweI GCATC 3 cut(s) 269, 1080, 1085
MaeI CTAG 1 cut(s) 1097
MaeII ACGT 1 cut(s) 485
MalI GATC 5 cut(s) 74, 160, 276, 297, 593
MboI GATC 5 cut(s) 72, 158, 274, 295, 591
MboII GAAGA 5 cut(s) 16, 328, 497, 779, 959
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 3 cut(s) 473, 550, 1361
MluCI AATT 9 cut(s) 180, 407, 540, 575, 684, 726, 751, 891, 1027
MlyI GAGTC 2 cut(s) 784, 905
MseI TTAA 5 cut(s) 227, 461, 539, 836, 1494
MslI CAYNNNNRTG 1 cut(s) 1193
MspCI CTTAAG 1 cut(s) 1493
Mva1269I GAATGC 1 cut(s) 1550
MvnI CGCG 1 cut(s) 1367
MwoI GCNNNNNNNGC 4 cut(s) 151, 172, 240, 1073
NdeI CATATG 1 cut(s) 940
NdeII GATC 5 cut(s) 72, 158, 274, 295, 591
NlaIII CATG 8 cut(s) 403, 604, 1058, 1083, 1172, 1192, 1430, 1438
NlaIV GGNNCC 4 cut(s) 548, 1358, 1359, 1453
NspI RCATGY 4 cut(s) 403, 1172, 1430, 1438
PceI AGGCCT 2 cut(s) 145, 1162
PctI GAATGC 1 cut(s) 1550
PfeI GAWTC 7 cut(s) 493, 700, 964, 1210, 1458, 1501, 1513
PflFI GACNNNGTC 1 cut(s) 516
PkrI GCNGC 6 cut(s) 18, 21, 67, 957, 1051, 1075
PleI GAGTC 2 cut(s) 784, 904
PpsI GAGTC 2 cut(s) 784, 904
PpuMI RGGWCCY 1 cut(s) 1261
PshBI ATTAAT 2 cut(s) 461, 539
PsiI TTATAA 1 cut(s) 1564
Psp5II RGGWCCY 1 cut(s) 1261
PspN4I GGNNCC 4 cut(s) 548, 1358, 1359, 1453
PspOMI GGGCCC 2 cut(s) 546, 1357
PspPPI RGGWCCY 1 cut(s) 1261
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 1 cut(s) 274
PsyI GACNNNGTC 1 cut(s) 516
RsaI GTAC 2 cut(s) 625, 1326
RsaNI GTAC 2 cut(s) 624, 1325
RseI CAYNNNNRTG 1 cut(s) 1193
SaqAI TTAA 5 cut(s) 227, 461, 539, 836, 1494
SatI GCNGC 6 cut(s) 17, 20, 66, 956, 1050, 1074
Sau3AI GATC 5 cut(s) 72, 158, 274, 295, 591
SchI GAGTC 2 cut(s) 784, 905
SduI GDGCHC 3 cut(s) 473, 550, 1361
SfaNI GCATC 3 cut(s) 269, 1080, 1085
SfcI CTRYAG 2 cut(s) 270, 678
SinI GGWCC 6 cut(s) 247, 500, 607, 1261, 1309, 1451
SmiMI CAYNNNNRTG 1 cut(s) 1193
SmlI CTYRAG 3 cut(s) 778, 1406, 1493
SmoI CTYRAG 3 cut(s) 778, 1406, 1493
Sse9I AATT 9 cut(s) 180, 407, 540, 575, 684, 726, 751, 891, 1027
SseBI AGGCCT 2 cut(s) 145, 1162
SsiI CCGC 1 cut(s) 1152
SspI AATATT 1 cut(s) 774
SspMI CTAG 1 cut(s) 1097
StuI AGGCCT 2 cut(s) 145, 1162
StyI CCWWGG 1 cut(s) 1447
TaaI ACNGT 1 cut(s) 623
TaiI ACGT 1 cut(s) 488
TaqI TCGA 1 cut(s) 530
TasI AATT 9 cut(s) 180, 407, 540, 575, 684, 726, 751, 891, 1027
TatI WGTACW 1 cut(s) 623
TfiI GAWTC 7 cut(s) 493, 700, 964, 1210, 1458, 1501, 1513
Tru1I TTAA 5 cut(s) 227, 461, 539, 836, 1494
Tru9I TTAA 5 cut(s) 227, 461, 539, 836, 1494
TscAI CASTG 3 cut(s) 639, 934, 1069
TseI GCWGC 6 cut(s) 16, 19, 65, 955, 1049, 1073
TspDTI ATGAA 8 cut(s) 17, 141, 189, 378, 927, 1006, 1033, 1205
TspRI CASTG 3 cut(s) 639, 934, 1069
Tth111I GACNNNGTC 1 cut(s) 516
Vha464I CTTAAG 1 cut(s) 1493
VpaK11BI GGWCC 6 cut(s) 247, 500, 607, 1261, 1309, 1451
VspI ATTAAT 2 cut(s) 461, 539
XceI RCATGY 4 cut(s) 403, 1172, 1430, 1438
XspI CTAG 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.