RLG00000028856

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001937
Physical Location & Seq
Reverse (-)
29361708 .. 29368942
7235 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028856

Sequence Viewer

Length: 1407 bp
ATGGAAGCAGAGTTAACAGTAGCTGCAAAGATGGTAGTACTGTCAGTATCGGTGGGTGGGCTTTTGTTCTTGTTTTCATATTTGTGTGAACTTCTGCTGTTGAGGCCTAAATCCATAAGATCAAAGCTGGAAAAGCAAGGAATTCGAGGGCCGCGTCCTACCTTTCTATTGGGAAACCTCCCTGAACAGAAGAGGCTCCAAAGCAAGCAGCAGGGTCAGTCAATAGCAACAAGGACTGACGATGATGATGATCATGCAGCTTCTGTTTCTCATCAATGGTATTTGAAAATCTTCCCCCACATAGAGCAATGGAGAAATGAATACGTTTACCAGGGTCTGGCCAGGCCGCGGTTACCAGGGTCAAGAGTGAAGGGCATGATGAACCTGGTAGTGGATTCTACAACCTCAATGCTAAGAACTTGGGAAAATGAAGTCCAAAGCCAGGGAGAACTTGCTGATATTAGAGTTGATGATCATTTGAGAAACTTATCAGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGCTGCAAGAAGTTTTAGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACAGAAAATAGAAAGATATGGATGTTGGAAAAGGAAACTCAATCACTGATATTGAAGGTGGTAAAACAACGTAATGAAGGTTCATTTGAGAAAGATTTGCTGCAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGACTTAAATGGCCTATCACACAATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCACTGCCCTTGTAACATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTACTTGAAATCTGTGGTGATAAGCCTCTAGATGCAGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGCACTCTTTCTATCAAGAGAGGTATTTGAAACTATCAAATTTAAAAACATTACATTTCCAAAGGGGACAATACTTCAAATACCAATCCCCTTCTTACACCAGAACCCTGATCTGTGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTGCCAATGGAATTGTCAAGGCTTGCAAGTCTCCACAGGCCTACATGCCATTCGGCGCAGGTTCTCGTATATGTGTTGGTCAACACTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATGATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGCATACCAGCATTCTCCTGCATATAACATTATTCTGGAGCCTAAATATGGTATTATTCTACAAATGCGAAGAGCGTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

469

Amino Acids

53.2

Weight (kDa)

9.2

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 124 - 450 9.2e-71 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1217
AccB1I GGYRCC 1 cut(s) 749
AccB7I CCANNNNNTGG 1 cut(s) 337
AccII CGCG 2 cut(s) 154, 349
AciI CCGC 4 cut(s) 152, 347, 349, 1013
AclWI GGATC 1 cut(s) 1144
AcoI YGGCCR 2 cut(s) 339, 829
AcsI RAATTY 2 cut(s) 141, 1055
AfaI GTAC 2 cut(s) 39, 918
AfiI CCNNNNNNNGG 5 cut(s) 337, 348, 391, 442, 1370
AflII CTTAAG 1 cut(s) 1271
AgsI TTSAA 7 cut(s) 286, 685, 746, 923, 1046, 1094, 1156
AjnI CCWGG 5 cut(s) 330, 341, 355, 384, 441
AleI CACNNNNGTG 1 cut(s) 983
AluBI AGCT 9 cut(s) 23, 127, 260, 512, 568, 864, 878, 899, 911
AluI AGCT 9 cut(s) 23, 127, 260, 512, 568, 864, 878, 899, 911
Alw21I GWGCWC 2 cut(s) 907, 978
Alw26I GTCTC 2 cut(s) 830, 1203
AlwI GGATC 1 cut(s) 1144
AlwNI CAGNNNCTG 3 cut(s) 23, 263, 337
Ama87I CYCGRG 1 cut(s) 906
AoxI GGCC 7 cut(s) 104, 149, 339, 344, 775, 829, 1206
ApeKI GCWGC 5 cut(s) 23, 208, 257, 568, 730
ApoI RAATTY 2 cut(s) 141, 1055
Asp700I GAANNNNTTC 1 cut(s) 290
AspLEI GCGC 1 cut(s) 1226
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 776, 944, 997
AvaI CYCGRG 1 cut(s) 906
BalI TGGCCA 2 cut(s) 341, 831
BanI GGYRCC 1 cut(s) 749
BauI CACGAG 1 cut(s) 900
Bbv12I GWGCWC 2 cut(s) 907, 978
BbvCI CCTCAGC 1 cut(s) 912
BbvI GCAGC 5 cut(s) 10, 220, 269, 555, 717
BccI CCATC 4 cut(s) 25, 589, 612, 891
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 5 cut(s) 332, 343, 357, 386, 443
BclI TGATCA 2 cut(s) 250, 472
BcoDI GTCTC 2 cut(s) 830, 1203
BfaI CTAG 2 cut(s) 875, 944
BfrI CTTAAG 1 cut(s) 1271
BfuAI ACCTGC 1 cut(s) 1217
BisI GCNGC 7 cut(s) 24, 152, 209, 258, 347, 569, 731
BlsI GCNGC 7 cut(s) 25, 153, 210, 259, 348, 570, 732
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 5 cut(s) 332, 343, 357, 386, 443
BmeT110I CYCGRG 1 cut(s) 906
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 3 cut(s) 197, 751, 1362
BmrFI CCNGG 5 cut(s) 332, 343, 357, 386, 443
BmsI GCATC 2 cut(s) 937, 1132
BpmI CTGGAG 1 cut(s) 1379
Bpu10I CCTNAGC 1 cut(s) 912
BsaBI GATNNNNATC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 4 cut(s) 331, 347, 356, 442
BsaXI ACNNNNNCTCC 2 cut(s) 864, 894
Bsc4I CCNNNNNNNGG 5 cut(s) 337, 348, 391, 442, 1370
Bse3DI GCAATG 1 cut(s) 314
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 5 cut(s) 332, 343, 357, 386, 443
BseDI CCNNGG 4 cut(s) 331, 347, 356, 442
BseGI GGATG 2 cut(s) 657, 883
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 5 cut(s) 337, 348, 391, 442, 1370
BseMI GCAATG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 903
BseXI GCAGC 5 cut(s) 10, 220, 269, 555, 717
BsgI GTGCAG 1 cut(s) 1002
Bsh1236I CGCG 2 cut(s) 154, 349
BshFI GGCC 7 cut(s) 106, 151, 341, 346, 777, 831, 1208
BshNI GGYRCC 1 cut(s) 749
BsiHKAI GWGCWC 2 cut(s) 907, 978
BsiHKCI CYCGRG 1 cut(s) 906
BslFI GGGAC 1 cut(s) 1096
BslI CCNNNNNNNGG 5 cut(s) 337, 348, 391, 442, 1370
BsmAI GTCTC 2 cut(s) 830, 1203
BsmFI GGGAC 1 cut(s) 1096
BsmI GAATGC 1 cut(s) 1333
BsnI GGCC 7 cut(s) 106, 151, 341, 346, 777, 831, 1208
Bso31I GGTCTC 1 cut(s) 830
BsoBI CYCGRG 1 cut(s) 906
Bsp1286I GDGCHC 2 cut(s) 907, 978
Bsp143I GATC 5 cut(s) 119, 250, 472, 1126, 1136
BspACI CCGC 4 cut(s) 152, 347, 349, 1013
BspANI GGCC 7 cut(s) 106, 151, 341, 346, 777, 831, 1208
BspCNI CTCAG 1 cut(s) 904
BspFNI CGCG 2 cut(s) 154, 349
BspLI GGNNCC 3 cut(s) 197, 751, 1362
BspMI ACCTGC 1 cut(s) 1217
BspPI GGATC 1 cut(s) 1144
BspQI GCTCTTC 1 cut(s) 1387
BspT107I GGYRCC 1 cut(s) 749
BspTI CTTAAG 1 cut(s) 1271
BspTNI GGTCTC 1 cut(s) 830
BsrDI GCAATG 1 cut(s) 314
BssECI CCNNGG 4 cut(s) 331, 347, 356, 442
BssMI GATC 5 cut(s) 119, 250, 472, 1126, 1136
BssSI CACGAG 1 cut(s) 900
Bst2BI CACGAG 1 cut(s) 900
Bst2UI CCWGG 5 cut(s) 332, 343, 357, 386, 443
Bst4CI ACNGT 2 cut(s) 19, 42
Bst6I CTCTTC 2 cut(s) 185, 1387
BstAFI CTTAAG 1 cut(s) 1271
BstC8I GCNNGC 6 cut(s) 206, 829, 866, 897, 1017, 1192
BstDEI CTNAG 2 cut(s) 413, 912
BstDSI CCRYGG 1 cut(s) 347
BstEII GGTNACC 1 cut(s) 351
BstF5I GGATG 2 cut(s) 657, 883
BstFNI CGCG 2 cut(s) 154, 349
BstHHI GCGC 1 cut(s) 1226
BstKTI GATC 5 cut(s) 122, 253, 475, 1129, 1139
BstMAI GTCTC 2 cut(s) 830, 1203
BstMBI GATC 5 cut(s) 119, 250, 472, 1126, 1136
BstMWI GCNNNNNNNGC 3 cut(s) 103, 133, 736
BstNI CCWGG 5 cut(s) 332, 343, 357, 386, 443
BstNSI RCATGY 1 cut(s) 1216
BstPI GGTNACC 1 cut(s) 351
BstSCI CCNGG 5 cut(s) 330, 341, 355, 384, 441
BstUI CGCG 2 cut(s) 154, 349
BstV1I GCAGC 5 cut(s) 10, 220, 269, 555, 717
BstX2I RGATCY 1 cut(s) 1136
BstYI RGATCY 1 cut(s) 1136
BsuRI GGCC 7 cut(s) 106, 151, 341, 346, 777, 831, 1208
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 2 cut(s) 657, 883
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 2 cut(s) 674, 840
BveI ACCTGC 1 cut(s) 1217
Cac8I GCNNGC 6 cut(s) 206, 829, 866, 897, 1017, 1192
CaiI CAGNNNCTG 3 cut(s) 23, 263, 337
CfoI GCGC 1 cut(s) 1226
Cfr13I GGNCC 1 cut(s) 149
Cfr42I CCGCGG 1 cut(s) 350
CseI GACGC 3 cut(s) 143, 992, 1386
CsiI ACCWGGT 1 cut(s) 384
Csp6I GTAC 2 cut(s) 38, 917
CspCI CAANNNNNGTGG 2 cut(s) 829, 864
CviAII CATG 5 cut(s) 254, 376, 833, 858, 1213
CviQI GTAC 2 cut(s) 38, 917
DdeI CTNAG 2 cut(s) 413, 912
DpnI GATC 5 cut(s) 121, 252, 474, 1128, 1138
DpnII GATC 5 cut(s) 119, 250, 472, 1126, 1136
DraI TTTAAA 1 cut(s) 1060
EaeI YGGCCR 2 cut(s) 339, 829
Eam1104I CTCTTC 2 cut(s) 185, 1387
EarI CTCTTC 2 cut(s) 185, 1387
Eco147I AGGCCT 2 cut(s) 106, 1208
Eco31I GGTCTC 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 906
Eco91I GGTNACC 1 cut(s) 351
EcoO65I GGTNACC 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 5 cut(s) 330, 341, 355, 384, 441
FaeI CATG 5 cut(s) 257, 379, 836, 861, 1216
FalI AAGNNNNNCTT 2 cut(s) 1292, 1324
FaqI GGGAC 1 cut(s) 1096
FatI CATG 5 cut(s) 253, 375, 832, 857, 1212
FauI CCCGC 1 cut(s) 1020
FbaI TGATCA 2 cut(s) 250, 472
Fnu4HI GCNGC 7 cut(s) 24, 152, 209, 258, 347, 569, 731
FokI GGATG 2 cut(s) 664, 870
Fsp4HI GCNGC 7 cut(s) 24, 152, 209, 258, 347, 569, 731
FspBI CTAG 2 cut(s) 875, 944
GlaI GCGC 1 cut(s) 1225
GluI GCNGC 7 cut(s) 24, 152, 209, 258, 347, 569, 731
GsuI CTGGAG 1 cut(s) 1379
HaeIII GGCC 7 cut(s) 106, 151, 341, 346, 777, 831, 1208
HgaI GACGC 3 cut(s) 143, 992, 1386
HhaI GCGC 1 cut(s) 1226
Hin1II CATG 5 cut(s) 257, 379, 836, 861, 1216
Hin6I GCGC 1 cut(s) 1224
HinP1I GCGC 1 cut(s) 1224
HincII GTYRAC 2 cut(s) 15, 1250
HindII GTYRAC 2 cut(s) 15, 1250
HindIII AAGCTT 1 cut(s) 510
HinfI GANTC 3 cut(s) 395, 988, 1291
HpaI GTTAAC 1 cut(s) 15
HphI GGTGA 3 cut(s) 776, 944, 997
Hpy166II GTNNAC 4 cut(s) 15, 89, 328, 1250
Hpy188I TCNGA 2 cut(s) 627, 1141
Hpy188III TCNNGA 7 cut(s) 363, 743, 887, 944, 992, 1032, 1358
Hpy8I GTNNAC 4 cut(s) 15, 89, 328, 1250
HpyAV CCTTC 5 cut(s) 364, 679, 701, 740, 1117
HpyCH4III ACNGT 2 cut(s) 19, 42
HpyCH4IV ACGT 2 cut(s) 324, 700
HpyCH4V TGCA 9 cut(s) 26, 257, 571, 733, 810, 950, 1019, 1194, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 133, 736
HpyF3I CTNAG 2 cut(s) 413, 912
HpySE526I ACGT 2 cut(s) 324, 700
Hsp92II CATG 5 cut(s) 257, 379, 836, 861, 1216
HspAI GCGC 1 cut(s) 1224
Ksp22I TGATCA 2 cut(s) 250, 472
KspAI GTTAAC 1 cut(s) 15
KspI CCGCGG 1 cut(s) 350
Kzo9I GATC 5 cut(s) 119, 250, 472, 1126, 1136
LguI GCTCTTC 1 cut(s) 1387
LmnI GCTCC 3 cut(s) 201, 861, 1360
Lsp1109I GCAGC 5 cut(s) 10, 220, 269, 555, 717
LweI GCATC 2 cut(s) 937, 1132
MabI ACCWGGT 1 cut(s) 384
MaeI CTAG 2 cut(s) 875, 944
MaeII ACGT 2 cut(s) 324, 700
MaeIII GTNAC 4 cut(s) 351, 764, 850, 870
MalI GATC 5 cut(s) 121, 252, 474, 1128, 1138
MboI GATC 5 cut(s) 119, 250, 472, 1126, 1136
MboII GAAGA 3 cut(s) 202, 283, 1404
MflI RGATCY 1 cut(s) 1136
MhlI GDGCHC 2 cut(s) 907, 978
MlsI TGGCCA 2 cut(s) 341, 831
MluCI AATT 5 cut(s) 141, 526, 558, 1055, 1179
MluNI TGGCCA 2 cut(s) 341, 831
MmeI TCCRAC 2 cut(s) 636, 650
Mox20I TGGCCA 2 cut(s) 341, 831
MroXI GAANNNNTTC 1 cut(s) 290
MscI TGGCCA 2 cut(s) 341, 831
MseI TTAA 4 cut(s) 14, 770, 1059, 1272
MslI CAYNNNNRTG 2 cut(s) 82, 983
Msp20I TGGCCA 2 cut(s) 341, 831
MspA1I CMGCKG 1 cut(s) 349
MspCI CTTAAG 1 cut(s) 1271
MspR9I CCNGG 5 cut(s) 332, 343, 357, 386, 443
Mva1269I GAATGC 1 cut(s) 1333
MvaI CCWGG 5 cut(s) 332, 343, 357, 386, 443
MvnI CGCG 2 cut(s) 154, 349
MwoI GCNNNNNNNGC 3 cut(s) 103, 133, 736
NdeII GATC 5 cut(s) 119, 250, 472, 1126, 1136
NlaIII CATG 5 cut(s) 257, 379, 836, 861, 1216
NlaIV GGNNCC 3 cut(s) 197, 751, 1362
NmuCI GTSAC 1 cut(s) 764
NspI RCATGY 1 cut(s) 1216
OliI CACNNNNGTG 1 cut(s) 983
PaeR7I CTCGAG 1 cut(s) 906
PceI AGGCCT 2 cut(s) 106, 1208
PciSI GCTCTTC 1 cut(s) 1387
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PctI GAATGC 1 cut(s) 1333
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 3 cut(s) 395, 988, 1291
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 7 cut(s) 25, 153, 210, 259, 348, 570, 732
Psp6I CCWGG 5 cut(s) 330, 341, 355, 384, 441
PspEI GGTNACC 1 cut(s) 351
PspGI CCWGG 5 cut(s) 330, 341, 355, 384, 441
PspN4I GGNNCC 3 cut(s) 197, 751, 1362
PspPI GGNCC 1 cut(s) 149
PspXI VCTCGAGB 1 cut(s) 906
PsrI GAACNNNNNNTAC 2 cut(s) 694, 726
PstNI CAGNNNCTG 3 cut(s) 23, 263, 337
PsuI RGATCY 1 cut(s) 1136
RsaI GTAC 2 cut(s) 39, 918
RsaNI GTAC 2 cut(s) 38, 917
RseI CAYNNNNRTG 2 cut(s) 82, 983
SacII CCGCGG 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 1387
SaqAI TTAA 4 cut(s) 14, 770, 1059, 1272
SatI GCNGC 7 cut(s) 24, 152, 209, 258, 347, 569, 731
Sau3AI GATC 5 cut(s) 119, 250, 472, 1126, 1136
Sau96I GGNCC 1 cut(s) 149
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 5 cut(s) 332, 343, 357, 386, 443
SduI GDGCHC 2 cut(s) 907, 978
SexAI ACCWGGT 1 cut(s) 384
SfaNI GCATC 2 cut(s) 937, 1132
Sfr274I CTCGAG 1 cut(s) 906
Sfr303I CCGCGG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 906
SmiMI CAYNNNNRTG 2 cut(s) 82, 983
SmlI CTYRAG 2 cut(s) 906, 1271
SmoI CTYRAG 2 cut(s) 906, 1271
Sse9I AATT 5 cut(s) 141, 526, 558, 1055, 1179
SseBI AGGCCT 2 cut(s) 106, 1208
SsiI CCGC 4 cut(s) 152, 347, 349, 1013
SspI AATATT 1 cut(s) 549
SspMI CTAG 2 cut(s) 875, 944
StuI AGGCCT 2 cut(s) 106, 1208
StyD4I CCNGG 5 cut(s) 330, 341, 355, 384, 441
TaaI ACNGT 2 cut(s) 19, 42
TaiI ACGT 2 cut(s) 327, 703
TaqI TCGA 3 cut(s) 145, 907, 961
TasI AATT 5 cut(s) 141, 526, 558, 1055, 1179
TatI WGTACW 1 cut(s) 37
TauI GCSGC 2 cut(s) 154, 349
TfiI GAWTC 3 cut(s) 395, 988, 1291
Tru1I TTAA 4 cut(s) 14, 770, 1059, 1272
Tru9I TTAA 4 cut(s) 14, 770, 1059, 1272
TscAI CASTG 2 cut(s) 681, 847
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 5 cut(s) 23, 208, 257, 568, 730
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 8 cut(s) 66, 333, 395, 444, 509, 702, 720, 983
TspRI CASTG 2 cut(s) 681, 847
Van91I CCANNNNNTGG 1 cut(s) 337
Vha464I CTTAAG 1 cut(s) 1271
XapI RAATTY 2 cut(s) 141, 1055
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 1 cut(s) 1216
XhoI CTCGAG 1 cut(s) 906
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 2 cut(s) 875, 944
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.