Rroxscaffold_4G00309370

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001937
Physical Location & Seq
Reverse (-)
31116809 .. 31121532
4724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309370.1

Sequence Viewer

Length: 1527 bp
ATGAAGATTTTGAAGCCGCAGCAAATGGAAGGTGAGAAAATGGAAGCAGAGCTATTAGTGGTAGCAGCAAAGATCATATTGTCGGTATTGGTGGGTGGGTTTCTGCTGTTGTTCTTGTATCTCTATGAACTACTGATATTGAGGCCTAAAGGGCTAAGATCAAAGCTCCAAAAGCAAGGAATTAGAGGGCCTCCTCCTTCATTTCTCTTGGGGAATATCCTTGCATTAAAGAGCCTTCAAAGCAAGGTCCAGTCAACTGCATCAAAGACTACAGATCCTCAAAAGCCCTATGATGATGATGATCGTGTAGCTTCTATTTGTCATCAATGGCCTTCAAAAATCTTCCCCCACATAGAGGAATGGAGAAATGAATATGGGCCAACATTTATGTATGCACTTGTCCACATGCAACAATTATGTATAACAGATGTAGAGCTATTGAAGGAACTTGGGCTTTGCAAGTCATTAATCTTGGGCACTCCTTCCATAACGTCCAATATTCTTGGACCTCTTCTGGGACAGGGTCTTTTATCATCGAATGGAGTAATTTGGGCCCATCAAAGGAAAACTATCGCTCCTGAATTATACCTTGCTAAGATCAAGAAAATGTTACTAAGACAACTGTTGAGCAGCATCCGTAGTCAGGTAGTGCTGTTTCTTATGCTTCCGCAGAAAATCCTCCCCAGGAGAAAACAATTTGAAAATTTCTCTGTTTTGCACCAGTATCTATATTCACCTTTGAAAAGATGGTTGCCAACAAACGAAAATAGAGAAATATGGAGATTGGAGAAGGAAATTGAGTCAATGATATTGAAGGTGGTAAAACAGCGCAGTGAAGCTTCGTATGAGAAAGATTTGCTGCAAATGATTCTTCAGGGTGCTGAAACTTCGGGTGAAAATGGCCTATCACATAAGAAGTTCATTGTTGATAATTGCAAAACCATATTCATTGCTGCCCATGAGACCACTGCCGTTGCAGCATCATGGAGCTTGATGCTACTAGCTTTACATCCAGATTGGCAAGCTCGTGTTCGTGCTGAGGTGCTCGAAATCTGCGGGGATAGGCCTCTACATGCAGATATGCTTCCAAGCATGAAAGTGCTCACAATGGTGATTCAGGAAGTATTGCGTCTGTACTCGCCTGTATTTTTTTTAGCAAGAGAGGTCTTTGAAACCATCACATTGAAAAACATTGTGATACCAAAGGGTGTAGTCCTTCAGATACCAGTACCCTTTCTACACCAGAACCCTGATATATGGGGCCCTGACGCGCATAAGTTCAATCCTGAAAGATTTGCCAATGGAGTTCTCAAGGCTTGCAACTCTCCACATGCCTACATGCCATTTGGACTTGGTCCCCGAATCTGTGTTGGTCAACATTTAGCAATAACAGAGCTTAAGGTGATTCTTTCTTTGATTCTATCAAAGTTTTGTTTTTCCCTCTCATCAGCATACCAGCATTCCCCTGCTTATAAGCTCTTAACGGAGCCTGAACATGGTGTCATTCTCCGTATGAGGAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

508

Amino Acids

57.66

Weight (kDa)

9.32

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 234 - 491 1.5e-63 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1473
AccII CGCG 1 cut(s) 1271
AciI CCGC 3 cut(s) 17, 668, 1056
AclWI GGATC 1 cut(s) 269
AcsI RAATTY 1 cut(s) 703
AcuI CTGAAG 2 cut(s) 857, 1202
AfaI GTAC 2 cut(s) 1136, 1230
AfiI CCNNNNNNNGG 5 cut(s) 355, 515, 561, 643, 1496
AflII CTTAAG 1 cut(s) 1397
AjnI CCWGG 1 cut(s) 683
AleI CACNNNNGTG 1 cut(s) 1109
Alw21I GWGCWC 2 cut(s) 1047, 1104
Alw26I GTCTC 1 cut(s) 956
AlwI GGATC 1 cut(s) 269
AoxI GGCC 8 cut(s) 143, 188, 329, 377, 552, 901, 1064, 1261
ApaI GGGCCC 2 cut(s) 556, 1265
ApeKI GCWGC 6 cut(s) 19, 65, 630, 859, 953, 977
ApoI RAATTY 1 cut(s) 703
AseI ATTAAT 1 cut(s) 467
AspLEI GCGC 2 cut(s) 831, 1273
AspS9I GGNCC 9 cut(s) 188, 247, 377, 506, 552, 553, 1261, 1262, 1355
AsuHPI GGTGA 5 cut(s) 44, 726, 905, 1123, 1414
AvaII GGWCC 3 cut(s) 247, 506, 1355
BaeGI GKGCMC 3 cut(s) 479, 556, 1265
BanII GRGCYC 2 cut(s) 556, 1265
BauI CACGAG 1 cut(s) 1026
Bbv12I GWGCWC 2 cut(s) 1047, 1104
BbvCI CCTCAGC 1 cut(s) 1038
BbvI GCAGC 6 cut(s) 31, 77, 642, 846, 940, 989
BccI CCATC 3 cut(s) 564, 741, 1184
BceAI ACGGC 1 cut(s) 956
BciT130I CCWGG 1 cut(s) 685
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 1 cut(s) 1001
BfmI CTRYAG 1 cut(s) 270
BfrI CTTAAG 1 cut(s) 1397
BglI GCCNNNNNGGC 1 cut(s) 151
BisI GCNGC 7 cut(s) 17, 20, 66, 631, 860, 954, 978
BlsI GCNGC 7 cut(s) 18, 21, 67, 632, 861, 955, 979
Bme1390I CCNGG 1 cut(s) 685
Bme18I GGWCC 3 cut(s) 247, 506, 1355
BmgT120I GGNCC 9 cut(s) 188, 247, 377, 506, 552, 553, 1261, 1262, 1355
BmiI GGNNCC 5 cut(s) 554, 1262, 1263, 1357, 1488
BmrFI CCNGG 1 cut(s) 685
BmsI GCATC 4 cut(s) 269, 642, 984, 989
Bpu10I CCTNAGC 1 cut(s) 1038
BpuEI CTTGAG 1 cut(s) 1295
BsaBI GATNNNNATC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 1 cut(s) 683
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 5 cut(s) 355, 515, 561, 643, 1496
Bse1I ACTGG 3 cut(s) 250, 721, 1226
Bse3DI GCAATG 1 cut(s) 948
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 1 cut(s) 683
BseGI GGATG 2 cut(s) 633, 1009
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 5 cut(s) 355, 515, 561, 643, 1496
BseMI GCAATG 1 cut(s) 948
BseMII CTCAG 1 cut(s) 1029
BseNI ACTGG 3 cut(s) 250, 721, 1226
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 3 cut(s) 479, 556, 1265
BseXI GCAGC 6 cut(s) 31, 77, 642, 846, 940, 989
Bsh1236I CGCG 1 cut(s) 1271
BshFI GGCC 8 cut(s) 145, 190, 331, 379, 554, 903, 1066, 1263
BsiHKAI GWGCWC 2 cut(s) 1047, 1104
BslFI GGGAC 2 cut(s) 531, 1341
BslI CCNNNNNNNGG 5 cut(s) 355, 515, 561, 643, 1496
BsmAI GTCTC 1 cut(s) 956
BsmFI GGGAC 2 cut(s) 531, 1341
BsmI GAATGC 1 cut(s) 1459
BsnI GGCC 8 cut(s) 145, 190, 331, 379, 554, 903, 1066, 1263
Bso31I GGTCTC 1 cut(s) 956
Bsp120I GGGCCC 2 cut(s) 552, 1261
Bsp1286I GDGCHC 5 cut(s) 479, 556, 1047, 1104, 1265
Bsp143I GATC 5 cut(s) 72, 158, 274, 301, 597
BspACI CCGC 3 cut(s) 17, 668, 1056
BspANI GGCC 8 cut(s) 145, 190, 331, 379, 554, 903, 1066, 1263
BspCNI CTCAG 1 cut(s) 1030
BspFNI CGCG 1 cut(s) 1271
BspLI GGNNCC 5 cut(s) 554, 1262, 1263, 1357, 1488
BspPI GGATC 1 cut(s) 269
BspTI CTTAAG 1 cut(s) 1397
BspTNI GGTCTC 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 948
BsrI ACTGG 3 cut(s) 250, 721, 1226
BssECI CCNNGG 1 cut(s) 683
BssMI GATC 5 cut(s) 72, 158, 274, 301, 597
BssSI CACGAG 1 cut(s) 1026
Bst2BI CACGAG 1 cut(s) 1026
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 1 cut(s) 624
Bst6I CTCTTC 1 cut(s) 516
BstAFI CTTAAG 1 cut(s) 1397
BstC8I GCNNGC 2 cut(s) 1023, 1318
BstDEI CTNAG 4 cut(s) 155, 594, 614, 1038
BstF5I GGATG 2 cut(s) 633, 1009
BstFNI CGCG 1 cut(s) 1271
BstHHI GCGC 2 cut(s) 831, 1273
BstKTI GATC 5 cut(s) 75, 161, 277, 304, 600
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 5 cut(s) 72, 158, 274, 301, 597
BstMWI GCNNNNNNNGC 4 cut(s) 151, 172, 240, 977
BstNI CCWGG 1 cut(s) 685
BstNSI RCATGY 4 cut(s) 409, 1076, 1334, 1342
BstSCI CCNGG 1 cut(s) 683
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 3 cut(s) 479, 556, 1265
BstUI CGCG 1 cut(s) 1271
BstV1I GCAGC 6 cut(s) 31, 77, 642, 846, 940, 989
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 8 cut(s) 145, 190, 331, 379, 554, 903, 1066, 1263
BtsCI GGATG 2 cut(s) 633, 1009
BtsI GCAGTG 2 cut(s) 838, 966
BtsIMutI CAGTG 2 cut(s) 838, 966
Cac8I GCNNGC 2 cut(s) 1023, 1318
CfoI GCGC 2 cut(s) 831, 1273
Cfr13I GGNCC 9 cut(s) 188, 247, 377, 506, 552, 553, 1261, 1262, 1355
CseI GACGC 2 cut(s) 1118, 1277
Csp6I GTAC 2 cut(s) 1135, 1229
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviAII CATG 8 cut(s) 406, 959, 984, 1073, 1093, 1331, 1339, 1496
CviQI GTAC 2 cut(s) 1135, 1229
DdeI CTNAG 4 cut(s) 155, 594, 614, 1038
DpnI GATC 5 cut(s) 74, 160, 276, 303, 599
DpnII GATC 5 cut(s) 72, 158, 274, 301, 597
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco147I AGGCCT 2 cut(s) 145, 1066
Eco24I GRGCYC 2 cut(s) 556, 1265
Eco31I GGTCTC 1 cut(s) 956
Eco47I GGWCC 3 cut(s) 247, 506, 1355
Eco57I CTGAAG 2 cut(s) 857, 1202
EcoO109I RGGNCCY 3 cut(s) 188, 1261, 1262
EcoRII CCWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 556, 1265
FaeI CATG 8 cut(s) 409, 962, 987, 1076, 1096, 1334, 1342, 1499
FaqI GGGAC 2 cut(s) 531, 1341
FatI CATG 8 cut(s) 405, 958, 983, 1072, 1092, 1330, 1338, 1495
FauI CCCGC 1 cut(s) 1049
Fnu4HI GCNGC 7 cut(s) 17, 20, 66, 631, 860, 954, 978
FokI GGATG 2 cut(s) 620, 996
FriOI GRGCYC 2 cut(s) 556, 1265
Fsp4HI GCNGC 7 cut(s) 17, 20, 66, 631, 860, 954, 978
FspBI CTAG 1 cut(s) 1001
GlaI GCGC 2 cut(s) 830, 1272
GluI GCNGC 7 cut(s) 17, 20, 66, 631, 860, 954, 978
HaeIII GGCC 8 cut(s) 145, 190, 331, 379, 554, 903, 1066, 1263
HgaI GACGC 2 cut(s) 1118, 1277
HhaI GCGC 2 cut(s) 831, 1273
Hin1II CATG 8 cut(s) 409, 962, 987, 1076, 1096, 1334, 1342, 1499
Hin6I GCGC 2 cut(s) 829, 1271
HinP1I GCGC 2 cut(s) 829, 1271
HincII GTYRAC 2 cut(s) 255, 1376
HindII GTYRAC 2 cut(s) 255, 1376
HindIII AAGCTT 1 cut(s) 837
HinfI GANTC 6 cut(s) 800, 868, 1114, 1362, 1405, 1417
HphI GGTGA 5 cut(s) 44, 726, 905, 1123, 1414
Hpy166II GTNNAC 3 cut(s) 255, 403, 1376
Hpy188I TCNGA 1 cut(s) 1221
Hpy188III TCNNGA 5 cut(s) 578, 601, 1013, 1118, 1286
Hpy8I GTNNAC 3 cut(s) 255, 403, 1376
HpyAV CCTTC 9 cut(s) 23, 207, 245, 342, 436, 492, 784, 808, 1226
HpyCH4III ACNGT 1 cut(s) 624
HpyCH4IV ACGT 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 172, 240, 977
HpyF3I CTNAG 4 cut(s) 155, 594, 614, 1038
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 8 cut(s) 409, 962, 987, 1076, 1096, 1334, 1342, 1499
HspAI GCGC 2 cut(s) 829, 1271
Kzo9I GATC 5 cut(s) 72, 158, 274, 301, 597
LmnI GCTCC 4 cut(s) 171, 580, 987, 1486
Lsp1109I GCAGC 6 cut(s) 31, 77, 642, 846, 940, 989
LweI GCATC 4 cut(s) 269, 642, 984, 989
MaeI CTAG 1 cut(s) 1001
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 1 cut(s) 609
MalI GATC 5 cut(s) 74, 160, 276, 303, 599
MboI GATC 5 cut(s) 72, 158, 274, 301, 597
MboII GAAGA 4 cut(s) 16, 334, 503, 863
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 5 cut(s) 479, 556, 1047, 1104, 1265
MluCI AATT 8 cut(s) 180, 413, 546, 581, 695, 703, 795, 931
MlyI GAGTC 1 cut(s) 809
MseI TTAA 4 cut(s) 227, 467, 1398, 1481
MslI CAYNNNNRTG 2 cut(s) 1097, 1109
MspCI CTTAAG 1 cut(s) 1397
MspR9I CCNGG 1 cut(s) 685
Mva1269I GAATGC 1 cut(s) 1459
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 1 cut(s) 1271
MwoI GCNNNNNNNGC 4 cut(s) 151, 172, 240, 977
NdeII GATC 5 cut(s) 72, 158, 274, 301, 597
NlaIII CATG 8 cut(s) 409, 962, 987, 1076, 1096, 1334, 1342, 1499
NlaIV GGNNCC 5 cut(s) 554, 1262, 1263, 1357, 1488
NspI RCATGY 4 cut(s) 409, 1076, 1334, 1342
OliI CACNNNNGTG 1 cut(s) 1109
PceI AGGCCT 2 cut(s) 145, 1066
PctI GAATGC 1 cut(s) 1459
PfeI GAWTC 5 cut(s) 868, 1114, 1362, 1405, 1417
PflFI GACNNNGTC 2 cut(s) 522, 1353
PkrI GCNGC 7 cut(s) 18, 21, 67, 632, 861, 955, 979
PleI GAGTC 1 cut(s) 808
PpsI GAGTC 1 cut(s) 808
PshBI ATTAAT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 1473
Psp6I CCWGG 1 cut(s) 683
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 5 cut(s) 554, 1262, 1263, 1357, 1488
PspOMI GGGCCC 2 cut(s) 552, 1261
PspPI GGNCC 9 cut(s) 188, 247, 377, 506, 552, 553, 1261, 1262, 1355
PsuI RGATCY 1 cut(s) 274
PsyI GACNNNGTC 2 cut(s) 522, 1353
RsaI GTAC 2 cut(s) 1136, 1230
RsaNI GTAC 2 cut(s) 1135, 1229
RseI CAYNNNNRTG 2 cut(s) 1097, 1109
SaqAI TTAA 4 cut(s) 227, 467, 1398, 1481
SatI GCNGC 7 cut(s) 17, 20, 66, 631, 860, 954, 978
Sau3AI GATC 5 cut(s) 72, 158, 274, 301, 597
Sau96I GGNCC 9 cut(s) 188, 247, 377, 506, 552, 553, 1261, 1262, 1355
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 1 cut(s) 685
SduI GDGCHC 5 cut(s) 479, 556, 1047, 1104, 1265
SfaNI GCATC 4 cut(s) 269, 642, 984, 989
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 3 cut(s) 247, 506, 1355
SmiMI CAYNNNNRTG 2 cut(s) 1097, 1109
SmlI CTYRAG 2 cut(s) 1310, 1397
SmoI CTYRAG 2 cut(s) 1310, 1397
Sse9I AATT 8 cut(s) 180, 413, 546, 581, 695, 703, 795, 931
SseBI AGGCCT 2 cut(s) 145, 1066
SsiI CCGC 3 cut(s) 17, 668, 1056
SspI AATATT 1 cut(s) 499
SspMI CTAG 1 cut(s) 1001
StuI AGGCCT 2 cut(s) 145, 1066
StyD4I CCNGG 1 cut(s) 683
TaaI ACNGT 1 cut(s) 624
TaiI ACGT 1 cut(s) 494
TaqI TCGA 2 cut(s) 536, 1047
TasI AATT 8 cut(s) 180, 413, 546, 581, 695, 703, 795, 931
TatI WGTACW 1 cut(s) 1134
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 5 cut(s) 868, 1114, 1362, 1405, 1417
Tru1I TTAA 4 cut(s) 227, 467, 1398, 1481
Tru9I TTAA 4 cut(s) 227, 467, 1398, 1481
TscAI CASTG 2 cut(s) 838, 973
TseI GCWGC 6 cut(s) 19, 65, 630, 859, 953, 977
TspDTI ATGAA 7 cut(s) 17, 141, 189, 384, 910, 937, 1109
TspGWI ACGGA 3 cut(s) 626, 1499, 1499
TspRI CASTG 2 cut(s) 838, 973
Tth111I GACNNNGTC 2 cut(s) 522, 1353
Vha464I CTTAAG 1 cut(s) 1397
VpaK11BI GGWCC 3 cut(s) 247, 506, 1355
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 1 cut(s) 703
XceI RCATGY 4 cut(s) 409, 1076, 1334, 1342
XspI CTAG 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.