Rorug01G0175800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001937
Physical Location & Seq
Reverse (-)
23903902 .. 23906583
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0175800.1

Sequence Viewer

Length: 867 bp
ATGGAAGATGAATGGAAAATTACTGAGGCCATGCCACAGCTCATGGTGGCTTCAGAAAAAGCATTTGAAGCTGCAGATTTCTATGAACTCTTATTTGGAGCACAGAAAACTCGATACAATGAGTTTCCCATGCAGAGTCGAACGTCTGACAGGTACTGCCGGGTTGCATGTGTGGAGCTCACAATAGGTTCCAGCTCTCTTGTGGTGAGGGATCTTGACAGTATAAACAGTCGGGTTGTGAGAGATGTAAATTCATGGGTTTCCACTTGCCTCCGCCTTCAATGCTTGAACCTGGACCTTATGATGGCAAGGGCTGTCAGTATGGGCTGCACGGTTCTTAAAGAAATTTTGGAGGATCCAGTTCGTCCTGGTGTGATGGTCGGCATTATAAGAGATCCATTTGGCCATGTATGGGAGCTCAGCGACTCATCTTATGGCGTCTCAGAAGAGGGTTTCATGAACACTGAGTGCATGAACGTGTTGCTTCAAGGTTGGAGCAAGAGTAAGAGGGAGGAGAACAAAGGGCTGTTTGAAACCTATGATTTCACACCAATGGTTGGGTCCTGTGAGACAAAGAAGAATGCAGCACATATAGTCAGTACGTGCAGACATCGAGGTCCCTTATTTGACCCCATTCTGTTTGATTGCAAATACATGGCAGTCTCGACAATGCTACAAGAGCAGCTGAATTACAAAGGAACACTTGTAGGGCTTGCTGATCCATCTACTTCCTTCAAAGACAGAGAAAGATGCATCGAAAAAGGAGCAGATTTTGTTGTGGAAAAGCCCCTGAATTTGAAAAAGCTTACCCACATTATGTTCAAGGCTCTGAAGAAGAAGAAAAAGGAGAAGATGAAGGAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

288

Amino Acids

32.84

Weight (kDa)

6.9

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 13 - 72 2.4e-07 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 389
AasI GACNNNNNNGTC 1 cut(s) 615
AccB7I CCANNNNNTGG 1 cut(s) 557
AciI CCGC 1 cut(s) 274
AclWI GGATC 5 cut(s) 219, 350, 363, 389, 713
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 3 cut(s) 250, 345, 793
AcuI CTGAAG 2 cut(s) 36, 851
AcyI GRCGYC 1 cut(s) 438
AdeI CACNNNGTG 1 cut(s) 468
AfaI GTAC 2 cut(s) 155, 601
AfiI CCNNNNNNNGG 3 cut(s) 304, 412, 557
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 8 cut(s) 68, 281, 289, 488, 533, 736, 799, 823
AjnI CCWGG 2 cut(s) 291, 367
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 7 cut(s) 40, 71, 178, 195, 418, 685, 805
AluI AGCT 7 cut(s) 40, 71, 178, 195, 418, 685, 805
Alw21I GWGCWC 3 cut(s) 103, 180, 420
Alw26I GTCTC 3 cut(s) 445, 563, 667
AlwI GGATC 5 cut(s) 219, 350, 363, 389, 713
AlwNI CAGNNNCTG 1 cut(s) 156
AoxI GGCC 2 cut(s) 27, 403
ApeKI GCWGC 4 cut(s) 71, 327, 584, 682
ApoI RAATTY 3 cut(s) 250, 345, 793
AspS9I GGNCC 3 cut(s) 295, 561, 617
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 217
AvaII GGWCC 3 cut(s) 295, 561, 617
BalI TGGCCA 1 cut(s) 405
BamHI GGATCC 1 cut(s) 355
BanII GRGCYC 2 cut(s) 180, 420
Bbv12I GWGCWC 3 cut(s) 103, 180, 420
BbvI GCAGC 4 cut(s) 58, 314, 596, 694
BccI CCATC 3 cut(s) 298, 370, 730
BciT130I CCWGG 2 cut(s) 293, 369
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 3 cut(s) 445, 563, 667
BfmI CTRYAG 1 cut(s) 72
BisI GCNGC 4 cut(s) 72, 328, 585, 683
BlpI GCTNAGC 1 cut(s) 419
BlsI GCNGC 4 cut(s) 73, 329, 586, 684
Bme1390I CCNGG 3 cut(s) 161, 293, 369
Bme18I GGWCC 3 cut(s) 295, 561, 617
BmgT120I GGNCC 3 cut(s) 295, 561, 617
BmiI GGNNCC 4 cut(s) 190, 357, 562, 619
BmrFI CCNGG 3 cut(s) 161, 293, 369
BmsI GCATC 2 cut(s) 740, 762
Bpu1102I GCTNAGC 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 438
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bsc4I CCNNNNNNNGG 3 cut(s) 304, 412, 557
Bse1I ACTGG 1 cut(s) 359
BseBI CCWGG 2 cut(s) 293, 369
BseLI CCNNNNNNNGG 3 cut(s) 304, 412, 557
BseMII CTCAG 4 cut(s) 15, 433, 456, 456
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 4 cut(s) 58, 314, 596, 694
BsgI GTGCAG 2 cut(s) 313, 625
BshFI GGCC 2 cut(s) 29, 405
BsiHKAI GWGCWC 3 cut(s) 103, 180, 420
BsiSI CCGG 1 cut(s) 160
BslFI GGGAC 1 cut(s) 603
BslI CCNNNNNNNGG 3 cut(s) 304, 412, 557
BsmAI GTCTC 3 cut(s) 445, 563, 667
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 603
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 2 cut(s) 29, 405
Bsp1286I GDGCHC 3 cut(s) 103, 180, 420
Bsp143I GATC 4 cut(s) 211, 355, 394, 718
Bsp1720I GCTNAGC 1 cut(s) 419
BspACI CCGC 1 cut(s) 274
BspANI GGCC 2 cut(s) 29, 405
BspCNI CTCAG 4 cut(s) 16, 432, 455, 457
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 4 cut(s) 190, 357, 562, 619
BspMAI CTGCAG 1 cut(s) 76
BspPI GGATC 5 cut(s) 219, 350, 363, 389, 713
BsrI ACTGG 1 cut(s) 359
BssMI GATC 4 cut(s) 211, 355, 394, 718
BssNI GRCGYC 1 cut(s) 438
Bst2UI CCWGG 2 cut(s) 293, 369
Bst4CI ACNGT 3 cut(s) 221, 230, 334
Bst6I CTCTTC 1 cut(s) 441
BstACI GRCGYC 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 4 cut(s) 24, 419, 442, 465
BstKTI GATC 4 cut(s) 214, 358, 397, 721
BstMAI GTCTC 3 cut(s) 445, 563, 667
BstMBI GATC 4 cut(s) 211, 355, 394, 718
BstMWI GCNNNNNNNGC 3 cut(s) 68, 282, 679
BstNI CCWGG 2 cut(s) 293, 369
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 3 cut(s) 159, 291, 367
BstSFI CTRYAG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 58, 314, 596, 694
BstX2I RGATCY 3 cut(s) 211, 355, 394
BstYI RGATCY 3 cut(s) 211, 355, 394
BsuRI GGCC 2 cut(s) 29, 405
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 714
CaiI CAGNNNCTG 1 cut(s) 156
CciI TCATGA 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 295, 561, 617
CseI GACGC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 154, 600
CviAII CATG 9 cut(s) 31, 43, 130, 168, 255, 407, 457, 472, 655
CviQI GTAC 2 cut(s) 154, 600
DdeI CTNAG 4 cut(s) 24, 419, 442, 465
DpnI GATC 4 cut(s) 213, 357, 396, 720
DpnII GATC 4 cut(s) 211, 355, 394, 718
DraIII CACNNNGTG 1 cut(s) 468
DrdI GACNNNNNNGTC 1 cut(s) 615
DseDI GACNNNNNNGTC 1 cut(s) 615
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
EciI GGCGGA 1 cut(s) 263
Ecl136II GAGCTC 2 cut(s) 178, 418
Eco24I GRGCYC 2 cut(s) 180, 420
Eco47I GGWCC 3 cut(s) 295, 561, 617
Eco53kI GAGCTC 2 cut(s) 178, 418
Eco57I CTGAAG 2 cut(s) 36, 851
EcoICRI GAGCTC 2 cut(s) 178, 418
EcoO109I RGGNCCY 2 cut(s) 561, 617
EcoRII CCWGG 2 cut(s) 291, 367
EcoT22I ATGCAT 1 cut(s) 755
EcoT38I GRGCYC 2 cut(s) 180, 420
Esp3I CGTCTC 1 cut(s) 445
FaeI CATG 9 cut(s) 34, 46, 133, 171, 258, 410, 460, 475, 658
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FaqI GGGAC 1 cut(s) 603
FatI CATG 9 cut(s) 30, 42, 129, 167, 254, 406, 456, 471, 654
Fnu4HI GCNGC 4 cut(s) 72, 328, 585, 683
FriOI GRGCYC 2 cut(s) 180, 420
Fsp4HI GCNGC 4 cut(s) 72, 328, 585, 683
GluI GCNGC 4 cut(s) 72, 328, 585, 683
HaeIII GGCC 2 cut(s) 29, 405
HapII CCGG 1 cut(s) 160
HgaI GACGC 1 cut(s) 427
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 9 cut(s) 34, 46, 133, 171, 258, 410, 460, 475, 658
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 2 cut(s) 136, 425
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 217
Hpy188I TCNGA 4 cut(s) 55, 148, 445, 831
Hpy188III TCNNGA 3 cut(s) 215, 457, 664
HpyAV CCTTC 3 cut(s) 287, 742, 850
HpyCH4III ACNGT 3 cut(s) 221, 230, 334
HpyCH4IV ACGT 3 cut(s) 143, 477, 602
HpyCH4V TGCA 9 cut(s) 74, 133, 167, 330, 471, 584, 606, 648, 753
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 282, 679
HpyF3I CTNAG 4 cut(s) 24, 419, 442, 465
HpySE526I ACGT 3 cut(s) 143, 477, 602
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 9 cut(s) 34, 46, 133, 171, 258, 410, 460, 475, 658
Kzo9I GATC 4 cut(s) 211, 355, 394, 718
LmnI GCTCC 5 cut(s) 98, 175, 415, 495, 764
Lsp1109I GCAGC 4 cut(s) 58, 314, 596, 694
LweI GCATC 2 cut(s) 740, 762
MaeII ACGT 3 cut(s) 143, 477, 602
MalI GATC 4 cut(s) 213, 357, 396, 720
MboI GATC 4 cut(s) 211, 355, 394, 718
MboII GAAGA 7 cut(s) 17, 458, 589, 844, 847, 850, 862
MflI RGATCY 3 cut(s) 211, 355, 394
MhlI GDGCHC 3 cut(s) 103, 180, 420
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 5 cut(s) 18, 250, 345, 688, 793
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 2 cut(s) 145, 419
MmeI TCCRAC 1 cut(s) 473
MnlI CCTC 8 cut(s) 19, 201, 281, 346, 442, 501, 505, 608
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 1 cut(s) 755
MscI TGGCCA 1 cut(s) 405
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 2 cut(s) 476, 551
Msp20I TGGCCA 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 3 cut(s) 161, 293, 369
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 2 cut(s) 293, 369
MwoI GCNNNNNNNGC 3 cut(s) 68, 282, 679
NciI CCSGG 1 cut(s) 161
NdeII GATC 4 cut(s) 211, 355, 394, 718
NlaIII CATG 9 cut(s) 34, 46, 133, 171, 258, 410, 460, 475, 658
NlaIV GGNNCC 4 cut(s) 190, 357, 562, 619
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 1 cut(s) 171
PagI TCATGA 1 cut(s) 456
PctI GAATGC 1 cut(s) 586
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 4 cut(s) 73, 329, 586, 684
PleI GAGTC 2 cut(s) 144, 419
PpsI GAGTC 2 cut(s) 144, 419
Ppu21I YACGTR 1 cut(s) 603
PpuMI RGGWCCY 2 cut(s) 561, 617
PsiI TTATAA 1 cut(s) 389
Psp124BI GAGCTC 2 cut(s) 180, 420
Psp5II RGGWCCY 2 cut(s) 561, 617
Psp6I CCWGG 2 cut(s) 291, 367
PspGI CCWGG 2 cut(s) 291, 367
PspN4I GGNNCC 4 cut(s) 190, 357, 562, 619
PspPI GGNCC 3 cut(s) 295, 561, 617
PspPPI RGGWCCY 2 cut(s) 561, 617
PstI CTGCAG 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 3 cut(s) 211, 355, 394
PvuII CAGCTG 1 cut(s) 685
RsaI GTAC 2 cut(s) 155, 601
RsaNI GTAC 2 cut(s) 154, 600
RseI CAYNNNNRTG 2 cut(s) 476, 551
SacI GAGCTC 2 cut(s) 180, 420
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 72, 328, 585, 683
Sau3AI GATC 4 cut(s) 211, 355, 394, 718
Sau96I GGNCC 3 cut(s) 295, 561, 617
SchI GAGTC 2 cut(s) 145, 419
ScrFI CCNGG 3 cut(s) 161, 293, 369
SduI GDGCHC 3 cut(s) 103, 180, 420
SfaNI GCATC 2 cut(s) 740, 762
SfcI CTRYAG 1 cut(s) 72
SinI GGWCC 3 cut(s) 295, 561, 617
SmiMI CAYNNNNRTG 2 cut(s) 476, 551
Sse9I AATT 5 cut(s) 18, 250, 345, 688, 793
SsiI CCGC 1 cut(s) 274
SstI GAGCTC 2 cut(s) 180, 420
StyD4I CCNGG 3 cut(s) 159, 291, 367
TaaI ACNGT 3 cut(s) 221, 230, 334
TaiI ACGT 3 cut(s) 146, 480, 605
TaqI TCGA 5 cut(s) 112, 139, 613, 665, 756
TasI AATT 5 cut(s) 18, 250, 345, 688, 793
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 469
TseI GCWGC 4 cut(s) 71, 327, 584, 682
TspDTI ATGAA 6 cut(s) 24, 99, 243, 445, 473, 488
TspRI CASTG 1 cut(s) 469
Van91I CCANNNNNTGG 1 cut(s) 557
VpaK11BI GGWCC 3 cut(s) 295, 561, 617
XapI RAATTY 3 cut(s) 250, 345, 793
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.