FvH4_7g09121

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001937
Physical Location & Seq
Forward (+)
8859585 .. 8864822
5238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09121.t4

Sequence Viewer

Length: 1596 bp
ATGGAAGGTGGGAAAATGGAAGCAGAGCTGTTTGTGATAGCAGCAAAGATCATATTGACAGTTTTGGTGGGTGGGTTTCTGTTCTTGTTCTTTTATCTCTATGAACTACTGATATTAAGGCCAGAAGGTCTAAGATCAAAGCTCCAAATTCAAGGAATTAAAGGGCCTCCTCCTTCATTTCTCTTGGGGAATGTCCTTGCATTGAAGCGCCTTCAGAACAAGGTCCAGTCAACAGCAACAAAGACTACAGATCCTCAGGATGAGGTTCATCATGAAGCTTCTATTTGTCATCAATGGCCTTCAAGGATCTTCCCCCACATAGAGGAATGGAGAAATGAATATGGGCCAACATTTATGTATGCACTTGCCCACATGCAACAATTATGTATAACAGATGTAGAGCTATTGAAGGAACTTGGGCTTTGCACATCGTTAATCTTAGGGACACCTTCCATAACGTCCAAGATTCTTGGCCCTCTACTCGGAAAGGGTCTTTTATCATCGAATGGCTTAATGTGGGCTCATCAAAGGAAAACTATAGCTCCTGAACTGTACCATGCTAAGGTCAAGGGTATGATGGACCTAATAGTGGAGTCTACAACCTCAATGCTAAAAACTTGGGAAAGTGAAATCCAAACTCAGGGCGGAACTGCAGAAATTAGAGTTGATGAGAATTTGAGAAAAATATCCGGAGATATAATTGCAAGAGCTTGTTTCCAGAGCAATTATAGTGAAGGAAAAGAAATATTCTCAAGACTTAGGACTCTACAAAAGATTTTGTCAAAGGGTATCATGGGGGTAGAAGGCTTAATCAGATGGTTGCCAACAAACGAAAATAGAGAGATATGGAGATTAGAGAAGGAAATTGAGTCAATGATATTGAAGGTGGTAAAACAGCGCAGTGAAGTTTCACATGAGAAAGATTTGCTGCAAATGATTCTTCAGGGTGCCGAAACTTTGGGTGAAAATGGCCTATCACATAAGAAATTCATCATTGATAATTGCAAGACCATATTCATTGCTGCGTATGAGACCACTGCCGTTGCAGCATCATGGAGCTTGATGCTACTAGCTGTACATGCAGATTGGCAAGCTCGTGTTCGTGCAGAAGTGCTCGAAATCTGCGGGGATAAACCTCTACATGCAGATATGCTTCCAAGCATGAAAGTGCTCACAATGGTGATTCAGGAAGTATTGCGTCTGTACTCGCCTGTATTTTTCCTAGCAAGAGAAGTCTTTGAAACTATCACATTGAAAAACGTTGTGATACCAAAGGGAGTGGTTCTTCAGATACCAATACCCTTTCTACACCAGGACCCTGATATATGGGGCCCTGATGCGCATAAGTTCAATCCTGAAAGATTTGCCAATGGAGTTCTCAAGGCTTGCAACTCCCCACAGGCATACATGCCATTTGGACTTGGTCCTCGAATCTGTGTTGGTCAACACCTAGCAATGACAGAGCTTAAGGTGATTCTCTCTTTGATTCTATCGAAGTTTTCTTTTTCACTCTCACCAGCATACCGACATTCCCCTGCTTATAAGCTCACAACGGGGCCTGAACATGGTGTCATTCTCCGTATGCGGAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

532

Amino Acids

59.75

Weight (kDa)

8.61

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 106 - 509 3.4e-77 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1542
Acc16I TGCGCA 1 cut(s) 1341
AccB1I GGYRCC 1 cut(s) 947
AccI GTMKAC 1 cut(s) 596
AccIII TCCGGA 1 cut(s) 689
AciI CCGC 3 cut(s) 645, 1125, 1585
AclI AACGTT 1 cut(s) 1260
AclWI GGATC 2 cut(s) 245, 314
AcsI RAATTY 3 cut(s) 147, 673, 986
AcuI CTGAAG 3 cut(s) 197, 926, 1271
AfaI GTAC 3 cut(s) 554, 1077, 1205
AfiI CCNNNNNNNGG 6 cut(s) 322, 482, 562, 589, 640, 1565
AflII CTTAAG 1 cut(s) 1466
AgsI TTSAA 8 cut(s) 152, 205, 303, 409, 883, 1241, 1255, 1351
AjnI CCWGG 1 cut(s) 1311
AleI CACNNNNGTG 1 cut(s) 1178
Alw21I GWGCWC 2 cut(s) 1116, 1173
Alw26I GTCTC 1 cut(s) 1025
AlwI GGATC 2 cut(s) 245, 314
Aor13HI TCCGGA 1 cut(s) 689
AoxI GGCC 8 cut(s) 119, 164, 296, 344, 472, 970, 1330, 1556
ApaI GGGCCC 1 cut(s) 1334
ApeKI GCWGC 4 cut(s) 41, 928, 1022, 1046
ApoI RAATTY 3 cut(s) 147, 673, 986
AspLEI GCGC 3 cut(s) 210, 900, 1342
AsuHPI GGTGA 4 cut(s) 974, 1192, 1483, 1506
AvaII GGWCC 4 cut(s) 223, 580, 1315, 1424
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 1334
BanI GGYRCC 1 cut(s) 947
BanII GRGCYC 2 cut(s) 523, 1334
BauI CACGAG 1 cut(s) 1095
Bbv12I GWGCWC 2 cut(s) 1116, 1173
BbvI GCAGC 4 cut(s) 53, 915, 1009, 1058
BccI CCATC 2 cut(s) 571, 810
BceAI ACGGC 1 cut(s) 1025
BciT130I CCWGG 1 cut(s) 1313
BcoDI GTCTC 1 cut(s) 1025
BfaI CTAG 3 cut(s) 1070, 1223, 1451
BfmI CTRYAG 3 cut(s) 246, 537, 651
BfoI RGCGCY 1 cut(s) 211
BfrI CTTAAG 1 cut(s) 1466
BisI GCNGC 4 cut(s) 42, 929, 1023, 1047
BlsI GCNGC 4 cut(s) 43, 930, 1024, 1048
Bme1390I CCNGG 1 cut(s) 1313
Bme18I GGWCC 4 cut(s) 223, 580, 1315, 1424
BmiI GGNNCC 5 cut(s) 949, 1317, 1331, 1332, 1557
BmrFI CCNGG 1 cut(s) 1313
BmsI GCATC 3 cut(s) 1053, 1058, 1327
Bpu10I CCTNAGC 1 cut(s) 561
BpuEI CTTGAG 2 cut(s) 736, 1364
BsaI GGTCTC 1 cut(s) 1025
BsaWI WCCGGW 1 cut(s) 689
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 6 cut(s) 322, 482, 562, 589, 640, 1565
Bse1I ACTGG 1 cut(s) 226
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 2 cut(s) 1017, 1461
BseAI TCCGGA 1 cut(s) 689
BseBI CCWGG 1 cut(s) 1313
BseGI GGATG 1 cut(s) 265
BseLI CCNNNNNNNGG 6 cut(s) 322, 482, 562, 589, 640, 1565
BseMI GCAATG 2 cut(s) 1017, 1461
BseMII CTCAG 2 cut(s) 269, 653
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 159
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 4 cut(s) 53, 915, 1009, 1058
BsgI GTGCAG 1 cut(s) 1125
BshFI GGCC 8 cut(s) 121, 166, 298, 346, 474, 972, 1332, 1558
BshNI GGYRCC 1 cut(s) 947
BsiHKAI GWGCWC 2 cut(s) 1116, 1173
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 6 cut(s) 322, 482, 562, 589, 640, 1565
BsmAI GTCTC 1 cut(s) 1025
BsmFI GGGAC 1 cut(s) 457
BsnI GGCC 8 cut(s) 121, 166, 298, 346, 474, 972, 1332, 1558
Bso31I GGTCTC 1 cut(s) 1025
Bsp120I GGGCCC 1 cut(s) 1330
Bsp1286I GDGCHC 4 cut(s) 523, 1116, 1173, 1334
Bsp13I TCCGGA 1 cut(s) 689
Bsp1407I TGTACA 1 cut(s) 1075
Bsp143I GATC 4 cut(s) 48, 134, 250, 306
BspACI CCGC 3 cut(s) 645, 1125, 1585
BspANI GGCC 8 cut(s) 121, 166, 298, 346, 474, 972, 1332, 1558
BspCNI CTCAG 2 cut(s) 268, 652
BspEI TCCGGA 1 cut(s) 689
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 5 cut(s) 949, 1317, 1331, 1332, 1557
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 2 cut(s) 245, 314
BspT107I GGYRCC 1 cut(s) 947
BspTI CTTAAG 1 cut(s) 1466
BspTNI GGTCTC 1 cut(s) 1025
BsrDI GCAATG 2 cut(s) 1017, 1461
BsrGI TGTACA 1 cut(s) 1075
BsrI ACTGG 1 cut(s) 226
BssMI GATC 4 cut(s) 48, 134, 250, 306
BssSI CACGAG 1 cut(s) 1095
Bst2BI CACGAG 1 cut(s) 1095
Bst2UI CCWGG 1 cut(s) 1313
Bst4CI ACNGT 2 cut(s) 61, 552
BstAFI CTTAAG 1 cut(s) 1466
BstAUI TGTACA 1 cut(s) 1075
BstC8I GCNNGC 2 cut(s) 1092, 1387
BstDEI CTNAG 6 cut(s) 131, 255, 439, 561, 639, 758
BstF5I GGATG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 3 cut(s) 210, 900, 1342
BstKTI GATC 4 cut(s) 51, 137, 253, 309
BstMAI GTCTC 1 cut(s) 1025
BstMBI GATC 4 cut(s) 48, 134, 250, 306
BstMWI GCNNNNNNNGC 2 cut(s) 1046, 1079
BstNI CCWGG 1 cut(s) 1313
BstNSI RCATGY 4 cut(s) 376, 1082, 1145, 1411
BstSCI CCNGG 1 cut(s) 1311
BstSFI CTRYAG 3 cut(s) 246, 537, 651
BstSLI GKGCMC 1 cut(s) 1334
BstV1I GCAGC 4 cut(s) 53, 915, 1009, 1058
BstX2I RGATCY 2 cut(s) 250, 306
BstYI RGATCY 2 cut(s) 250, 306
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 8 cut(s) 121, 166, 298, 346, 474, 972, 1332, 1558
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 2 cut(s) 907, 1035
BtsIMutI CAGTG 2 cut(s) 907, 1035
Cac8I GCNNGC 2 cut(s) 1092, 1387
CciI TCATGA 1 cut(s) 271
CfoI GCGC 3 cut(s) 210, 900, 1342
CseI GACGC 1 cut(s) 1187
Csp6I GTAC 3 cut(s) 553, 1076, 1204
CspCI CAANNNNNGTGG 4 cut(s) 1024, 1059, 1260, 1295
CviQI GTAC 3 cut(s) 553, 1076, 1204
DdeI CTNAG 6 cut(s) 131, 255, 439, 561, 639, 758
DpnI GATC 4 cut(s) 50, 136, 252, 308
DpnII GATC 4 cut(s) 48, 134, 250, 306
EciI GGCGGA 1 cut(s) 660
Eco24I GRGCYC 2 cut(s) 523, 1334
Eco31I GGTCTC 1 cut(s) 1025
Eco47I GGWCC 4 cut(s) 223, 580, 1315, 1424
Eco57I CTGAAG 3 cut(s) 197, 926, 1271
Eco81I CCTNAGG 1 cut(s) 255
EcoO109I RGGNCCY 5 cut(s) 164, 1315, 1330, 1331, 1556
EcoRII CCWGG 1 cut(s) 1311
EcoT38I GRGCYC 2 cut(s) 523, 1334
FaqI GGGAC 1 cut(s) 457
FauI CCCGC 1 cut(s) 1118
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 4 cut(s) 42, 929, 1023, 1047
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 2 cut(s) 523, 1334
Fsp4HI GCNGC 4 cut(s) 42, 929, 1023, 1047
FspAI RTGCGCAY 1 cut(s) 1341
FspBI CTAG 3 cut(s) 1070, 1223, 1451
FspI TGCGCA 1 cut(s) 1341
GlaI GCGC 3 cut(s) 209, 899, 1341
GluI GCNGC 4 cut(s) 42, 929, 1023, 1047
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 8 cut(s) 121, 166, 298, 346, 474, 972, 1332, 1558
HapII CCGG 1 cut(s) 690
HgaI GACGC 1 cut(s) 1187
HhaI GCGC 3 cut(s) 210, 900, 1342
Hin6I GCGC 3 cut(s) 208, 898, 1340
HinP1I GCGC 3 cut(s) 208, 898, 1340
HincII GTYRAC 2 cut(s) 231, 1445
HindII GTYRAC 2 cut(s) 231, 1445
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 9 cut(s) 466, 593, 763, 869, 937, 1183, 1431, 1474, 1486
HpaII CCGG 1 cut(s) 690
HphI GGTGA 4 cut(s) 974, 1192, 1483, 1506
Hpy166II GTNNAC 3 cut(s) 231, 597, 1445
Hpy188I TCNGA 4 cut(s) 216, 485, 815, 1290
Hpy188III TCNNGA 8 cut(s) 257, 272, 545, 690, 718, 753, 1187, 1355
Hpy8I GTNNAC 3 cut(s) 231, 597, 1445
HpyCH4III ACNGT 2 cut(s) 61, 552
HpyCH4IV ACGT 2 cut(s) 458, 1260
HpyF10VI GCNNNNNNNGC 2 cut(s) 1046, 1079
HpyF3I CTNAG 6 cut(s) 131, 255, 439, 561, 639, 758
HpySE526I ACGT 2 cut(s) 458, 1260
HspAI GCGC 3 cut(s) 208, 898, 1340
Kpn2I TCCGGA 1 cut(s) 689
Kzo9I GATC 4 cut(s) 48, 134, 250, 306
LmnI GCTCC 3 cut(s) 147, 547, 1056
Lsp1109I GCAGC 4 cut(s) 53, 915, 1009, 1058
LweI GCATC 3 cut(s) 1053, 1058, 1327
MaeI CTAG 3 cut(s) 1070, 1223, 1451
MaeII ACGT 2 cut(s) 458, 1260
MalI GATC 4 cut(s) 50, 136, 252, 308
MboI GATC 4 cut(s) 48, 134, 250, 306
MboII GAAGA 3 cut(s) 301, 932, 1277
MflI RGATCY 2 cut(s) 250, 306
MhlI GDGCHC 4 cut(s) 523, 1116, 1173, 1334
MlyI GAGTC 3 cut(s) 602, 757, 878
MnlI CCTC 9 cut(s) 177, 180, 256, 264, 316, 486, 613, 1146, 1437
MroI TCCGGA 1 cut(s) 689
MseI TTAA 6 cut(s) 116, 159, 434, 512, 809, 1467
MslI CAYNNNNRTG 2 cut(s) 1166, 1178
MspCI CTTAAG 1 cut(s) 1466
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 1 cut(s) 1313
MvaI CCWGG 1 cut(s) 1313
MwoI GCNNNNNNNGC 2 cut(s) 1046, 1079
NdeII GATC 4 cut(s) 48, 134, 250, 306
NlaIV GGNNCC 5 cut(s) 949, 1317, 1331, 1332, 1557
NsbI TGCGCA 1 cut(s) 1341
NspI RCATGY 4 cut(s) 376, 1082, 1145, 1411
OliI CACNNNNGTG 1 cut(s) 1178
PagI TCATGA 1 cut(s) 271
PfeI GAWTC 6 cut(s) 466, 937, 1183, 1431, 1474, 1486
PflFI GACNNNGTC 1 cut(s) 1422
PkrI GCNGC 4 cut(s) 43, 930, 1024, 1048
PleI GAGTC 3 cut(s) 601, 757, 877
PpsI GAGTC 3 cut(s) 601, 757, 877
PpuMI RGGWCCY 1 cut(s) 1315
PsiI TTATAA 1 cut(s) 1542
Psp1406I AACGTT 1 cut(s) 1260
Psp5II RGGWCCY 1 cut(s) 1315
Psp6I CCWGG 1 cut(s) 1311
PspGI CCWGG 1 cut(s) 1311
PspN4I GGNNCC 5 cut(s) 949, 1317, 1331, 1332, 1557
PspOMI GGGCCC 1 cut(s) 1330
PspPPI RGGWCCY 1 cut(s) 1315
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 2 cut(s) 250, 306
PsyI GACNNNGTC 1 cut(s) 1422
RsaI GTAC 3 cut(s) 554, 1077, 1205
RsaNI GTAC 3 cut(s) 553, 1076, 1204
RseI CAYNNNNRTG 2 cut(s) 1166, 1178
SaqAI TTAA 6 cut(s) 116, 159, 434, 512, 809, 1467
SatI GCNGC 4 cut(s) 42, 929, 1023, 1047
Sau3AI GATC 4 cut(s) 48, 134, 250, 306
SchI GAGTC 3 cut(s) 602, 757, 878
ScrFI CCNGG 1 cut(s) 1313
SduI GDGCHC 4 cut(s) 523, 1116, 1173, 1334
SfaNI GCATC 3 cut(s) 1053, 1058, 1327
SfcI CTRYAG 3 cut(s) 246, 537, 651
SinI GGWCC 4 cut(s) 223, 580, 1315, 1424
SmiMI CAYNNNNRTG 2 cut(s) 1166, 1178
SmlI CTYRAG 3 cut(s) 751, 1379, 1466
SmoI CTYRAG 3 cut(s) 751, 1379, 1466
SsiI CCGC 3 cut(s) 645, 1125, 1585
SspI AATATT 1 cut(s) 747
SspMI CTAG 3 cut(s) 1070, 1223, 1451
StyD4I CCNGG 1 cut(s) 1311
TaaI ACNGT 2 cut(s) 61, 552
TaiI ACGT 2 cut(s) 461, 1263
TaqI TCGA 4 cut(s) 503, 1116, 1429, 1493
TatI WGTACW 2 cut(s) 1075, 1203
TfiI GAWTC 6 cut(s) 466, 937, 1183, 1431, 1474, 1486
Tru1I TTAA 6 cut(s) 116, 159, 434, 512, 809, 1467
Tru9I TTAA 6 cut(s) 116, 159, 434, 512, 809, 1467
TscAI CASTG 2 cut(s) 907, 1042
TseI GCWGC 4 cut(s) 41, 928, 1022, 1046
TspDTI ATGAA 8 cut(s) 117, 165, 257, 288, 351, 979, 1006, 1178
TspGWI ACGGA 1 cut(s) 1568
TspRI CASTG 2 cut(s) 907, 1042
Tth111I GACNNNGTC 1 cut(s) 1422
Vha464I CTTAAG 1 cut(s) 1466
VpaK11BI GGWCC 4 cut(s) 223, 580, 1315, 1424
XapI RAATTY 3 cut(s) 147, 673, 986
XceI RCATGY 4 cut(s) 376, 1082, 1145, 1411
XmiI GTMKAC 1 cut(s) 596
XspI CTAG 3 cut(s) 1070, 1223, 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.