Rw1G015950

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001937
Physical Location & Seq
Forward (+)
35566596 .. 35576809
10214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015950.1

Sequence Viewer

Length: 1650 bp
ATGGAAGCAGAGTTAGCAGTAGCTGCAAAGATGGTAGTACTGTCAGTATTGGTGGGTGGGCTTTTGTTCTTGCCTAAATCCATGAGATCAAAGATGGAAAAGCAAGGAATTCGAGGGCCACGTCCTACCTTTCTATTGGGAAACCTCCCTGAACAGAAGAGGCTCCAAAGCAAGCAGCAGGGTCAGTCAATAGCAACAAGGACTGATGATCATGATGATCATGCAGCTTCTGTTTCTCATCAATGGCATTTGAAAATCTTCCCCCACAAAGAGCAATGGAGAAATGAATATGGTCCAATATTTTTGTGTTCATCTGCACACATACAACATTTAGTAATAACAGATATGGAAGTGGTGAAGGAAATTAGCTTGTGTAAGTCTCTAGCCTTGGGAAAACCTTCCGCAACATCCAAGGTTCTTGGGTCACTATTGGGAGAGGGCATTTTAGCGTCGAACGGATCAATGTGGGAGCATCAAAGGAAAATTATCGCTCCTGAACTATACCTTGATAAGGTGAAGGGCATGATGAACCTGTTAGTGGATTCTACAACCTCAATGCTAAGAACTTGGGAAAATGAAGTACAAAGCCAGGGAGAACTTGCTGATATTAGAGTTGATGATCATTTGAGAAACTTATCAGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGATGCAAGAAGTTTTGGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACCGAAAATAGAAAGATATGGATGTTGGAAAAGGAAACTCAATCACTGATATTGAAGGTGGTAAAACAACGTAATGAAGGTTCATATGAGAAAGATTTGCTGCAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGACTTAAATGGCCTATCACACAATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCACTGCCCTTGTAACATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTGCTTGAACTCTGTGGTGATAAGCCTCTAGATGCAGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGCACTCTTTCTATCAAGAGAGGTATTTGAAACTATCAAATTTAAAAACATTACATTTCCAAAGGGGACGCTACTTCAAATACCAATCCCCTTCTTACACCAGAACCCTGATCTGTGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTGCCAATGGAATTGTCAAGGCTTGCAAGTCTCCACAGGCCTACATGCCATTCGGAGCAGGTTCTCGTATATGTGTTGGTCAACACTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATGATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGCATACCAGCATTCTCCTGCATATAACATTATTCTGGAGCCTGAATATGGTGATCCTGGAGCAACAATCAAAGATGTAGCTAAACTGAGGGGGCTTGAGAATGAAGGTGATTTTGTTAGGTTGGTTTCTCTCTATTTTTTTGGTATGTATGCATGTAGGTCGCCCCTGTGCATGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

549

Amino Acids

61.87

Weight (kDa)

8.51

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 91 - 492 1.7e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1364
AccB1I GGYRCC 1 cut(s) 896
AciI CCGC 2 cut(s) 402, 1160
AclWI GGATC 3 cut(s) 466, 1291, 1517
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 2 cut(s) 108, 1202
AfaI GTAC 2 cut(s) 39, 582
AfiI CCNNNNNNNGG 4 cut(s) 511, 538, 1517, 1642
AflII CTTAAG 1 cut(s) 1418
AgsI TTSAA 7 cut(s) 253, 832, 893, 1070, 1193, 1241, 1303
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 2 cut(s) 588, 1525
AleI CACNNNNGTG 1 cut(s) 1130
AluBI AGCT 9 cut(s) 23, 227, 369, 659, 1011, 1025, 1046, 1058, 1550
AluI AGCT 9 cut(s) 23, 227, 369, 659, 1011, 1025, 1046, 1058, 1550
Alw21I GWGCWC 2 cut(s) 1054, 1125
Alw26I GTCTC 3 cut(s) 384, 977, 1350
AlwI GGATC 3 cut(s) 466, 1291, 1517
AlwNI CAGNNNCTG 2 cut(s) 23, 230
Ama87I CYCGRG 1 cut(s) 1053
AoxI GGCC 4 cut(s) 116, 922, 976, 1353
ApeKI GCWGC 4 cut(s) 23, 175, 224, 877
ApoI RAATTY 2 cut(s) 108, 1202
Asp700I GAANNNNTTC 2 cut(s) 257, 397
AspS9I GGNCC 2 cut(s) 116, 293
AsuHPI GGTGA 7 cut(s) 367, 526, 923, 1091, 1144, 1532, 1589
AvaI CYCGRG 1 cut(s) 1053
AvaII GGWCC 1 cut(s) 293
BalI TGGCCA 1 cut(s) 978
BanI GGYRCC 1 cut(s) 896
BauI CACGAG 1 cut(s) 1047
Bbv12I GWGCWC 2 cut(s) 1054, 1125
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 4 cut(s) 10, 187, 236, 864
BccI CCATC 5 cut(s) 25, 88, 736, 759, 1038
BcgI CGANNNNNNTGC 4 cut(s) 92, 126, 1611, 1645
BciT130I CCWGG 2 cut(s) 590, 1527
BclI TGATCA 3 cut(s) 208, 217, 619
BcoDI GTCTC 3 cut(s) 384, 977, 1350
BfaI CTAG 3 cut(s) 383, 1022, 1091
BfrI CTTAAG 1 cut(s) 1418
BfuAI ACCTGC 1 cut(s) 1364
BisI GCNGC 4 cut(s) 24, 176, 225, 878
BlsI GCNGC 4 cut(s) 25, 177, 226, 879
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 2 cut(s) 590, 1527
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 1053
BmgBI CACGTC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 116, 293
BmiI GGNNCC 3 cut(s) 164, 898, 1509
BmrFI CCNGG 2 cut(s) 590, 1527
BmsI GCATC 4 cut(s) 481, 705, 1084, 1279
BpmI CTGGAG 2 cut(s) 1526, 1548
Bpu10I CCTNAGC 1 cut(s) 1059
BpuEI CTTGAG 1 cut(s) 1586
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 3 cut(s) 387, 411, 589
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 538, 1517, 1642
Bse3DI GCAATG 1 cut(s) 281
BseBI CCWGG 2 cut(s) 590, 1527
BseDI CCNNGG 3 cut(s) 387, 411, 589
BseGI GGATG 3 cut(s) 407, 804, 1030
BseLI CCNNNNNNNGG 4 cut(s) 511, 538, 1517, 1642
BseMI GCAATG 1 cut(s) 281
BseMII CTCAG 2 cut(s) 1050, 1547
BseXI GCAGC 4 cut(s) 10, 187, 236, 864
BsgI GTGCAG 2 cut(s) 300, 1149
BshFI GGCC 4 cut(s) 118, 924, 978, 1355
BshNI GGYRCC 1 cut(s) 896
BsiHKAI GWGCWC 2 cut(s) 1054, 1125
BsiHKCI CYCGRG 1 cut(s) 1053
BslFI GGGAC 1 cut(s) 1243
BslI CCNNNNNNNGG 4 cut(s) 511, 538, 1517, 1642
BsmAI GTCTC 3 cut(s) 384, 977, 1350
BsmFI GGGAC 1 cut(s) 1243
BsmI GAATGC 1 cut(s) 1480
BsnI GGCC 4 cut(s) 118, 924, 978, 1355
Bso31I GGTCTC 1 cut(s) 977
BsoBI CYCGRG 1 cut(s) 1053
Bsp1286I GDGCHC 2 cut(s) 1054, 1125
Bsp143I GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
BspACI CCGC 2 cut(s) 402, 1160
BspANI GGCC 4 cut(s) 118, 924, 978, 1355
BspCNI CTCAG 2 cut(s) 1051, 1548
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 164, 898, 1509
BspMI ACCTGC 1 cut(s) 1364
BspPI GGATC 3 cut(s) 466, 1291, 1517
BspT107I GGYRCC 1 cut(s) 896
BspTI CTTAAG 1 cut(s) 1418
BspTNI GGTCTC 1 cut(s) 977
BsrDI GCAATG 1 cut(s) 281
BssECI CCNNGG 3 cut(s) 387, 411, 589
BssMI GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
BssSI CACGAG 1 cut(s) 1047
BssT1I CCWWGG 2 cut(s) 387, 411
Bst2BI CACGAG 1 cut(s) 1047
Bst2UI CCWGG 2 cut(s) 590, 1527
Bst4CI ACNGT 1 cut(s) 42
Bst6I CTCTTC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 1418
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 6 cut(s) 173, 976, 1013, 1044, 1164, 1339
BstDEI CTNAG 3 cut(s) 560, 1059, 1556
BstENI CCTNNNNNAGG 1 cut(s) 509
BstF5I GGATG 3 cut(s) 407, 804, 1030
BstKTI GATC 8 cut(s) 89, 211, 220, 461, 622, 1276, 1286, 1525
BstMAI GTCTC 3 cut(s) 384, 977, 1350
BstMBI GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
BstMWI GCNNNNNNNGC 3 cut(s) 14, 23, 883
BstNI CCWGG 2 cut(s) 590, 1527
BstNSI RCATGY 2 cut(s) 1363, 1626
BstSCI CCNGG 2 cut(s) 588, 1525
BstV1I GCAGC 4 cut(s) 10, 187, 236, 864
BstX2I RGATCY 1 cut(s) 1283
BstYI RGATCY 1 cut(s) 1283
BsuRI GGCC 4 cut(s) 118, 924, 978, 1355
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 3 cut(s) 407, 804, 1030
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 2 cut(s) 821, 987
BveI ACCTGC 1 cut(s) 1364
Cac8I GCNNGC 6 cut(s) 173, 976, 1013, 1044, 1164, 1339
CaiI CAGNNNCTG 2 cut(s) 23, 230
CciI TCATGA 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 116, 293
CseI GACGC 3 cut(s) 438, 1139, 1240
Csp6I GTAC 2 cut(s) 38, 581
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviAII CATG 9 cut(s) 82, 212, 221, 523, 980, 1005, 1360, 1623, 1642
CviQI GTAC 2 cut(s) 38, 581
DdeI CTNAG 3 cut(s) 560, 1059, 1556
DpnI GATC 8 cut(s) 88, 210, 219, 460, 621, 1275, 1285, 1524
DpnII GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
DraI TTTAAA 1 cut(s) 1207
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 2 cut(s) 387, 411
Eco147I AGGCCT 1 cut(s) 1355
Eco31I GGTCTC 1 cut(s) 977
Eco47I GGWCC 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 1053
EcoNI CCTNNNNNAGG 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 588, 1525
EcoT14I CCWWGG 2 cut(s) 387, 411
EcoT22I ATGCAT 1 cut(s) 1624
ErhI CCWWGG 2 cut(s) 387, 411
FaeI CATG 9 cut(s) 85, 215, 224, 526, 983, 1008, 1363, 1626, 1645
FalI AAGNNNNNCTT 2 cut(s) 1439, 1471
FaqI GGGAC 1 cut(s) 1243
FatI CATG 9 cut(s) 81, 211, 220, 522, 979, 1004, 1359, 1622, 1641
FauI CCCGC 1 cut(s) 1167
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 3 cut(s) 208, 217, 619
Fnu4HI GCNGC 4 cut(s) 24, 176, 225, 878
FokI GGATG 3 cut(s) 394, 811, 1017
Fsp4HI GCNGC 4 cut(s) 24, 176, 225, 878
FspBI CTAG 3 cut(s) 383, 1022, 1091
GluI GCNGC 4 cut(s) 24, 176, 225, 878
GsuI CTGGAG 2 cut(s) 1526, 1548
HaeIII GGCC 4 cut(s) 118, 924, 978, 1355
HgaI GACGC 3 cut(s) 438, 1139, 1240
Hin1II CATG 9 cut(s) 85, 215, 224, 526, 983, 1008, 1363, 1626, 1645
HincII GTYRAC 1 cut(s) 1397
HindII GTYRAC 1 cut(s) 1397
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 3 cut(s) 542, 1135, 1438
HphI GGTGA 7 cut(s) 367, 526, 923, 1091, 1144, 1532, 1589
Hpy166II GTNNAC 1 cut(s) 1397
Hpy188I TCNGA 3 cut(s) 774, 1288, 1370
Hpy188III TCNNGA 8 cut(s) 212, 494, 890, 1034, 1091, 1139, 1179, 1505
Hpy8I GTNNAC 1 cut(s) 1397
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 8 cut(s) 352, 408, 511, 826, 848, 887, 1264, 1568
HpyCH4III ACNGT 1 cut(s) 42
HpyCH4IV ACGT 2 cut(s) 121, 847
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 23, 883
HpyF3I CTNAG 3 cut(s) 560, 1059, 1556
HpySE526I ACGT 2 cut(s) 121, 847
Hsp92II CATG 9 cut(s) 85, 215, 224, 526, 983, 1008, 1363, 1626, 1645
Ksp22I TGATCA 3 cut(s) 208, 217, 619
Kzo9I GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
LmnI GCTCC 7 cut(s) 168, 469, 496, 1008, 1370, 1507, 1529
Lsp1109I GCAGC 4 cut(s) 10, 187, 236, 864
LweI GCATC 4 cut(s) 481, 705, 1084, 1279
MaeI CTAG 3 cut(s) 383, 1022, 1091
MaeII ACGT 2 cut(s) 121, 847
MaeIII GTNAC 4 cut(s) 423, 911, 997, 1017
MalI GATC 8 cut(s) 88, 210, 219, 460, 621, 1275, 1285, 1524
MboI GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
MboII GAAGA 3 cut(s) 169, 250, 724
MflI RGATCY 1 cut(s) 1283
MhlI GDGCHC 2 cut(s) 1054, 1125
MlsI TGGCCA 1 cut(s) 978
MluCI AATT 7 cut(s) 108, 363, 483, 673, 705, 1202, 1326
MluNI TGGCCA 1 cut(s) 978
MmeI TCCRAC 2 cut(s) 783, 797
Mox20I TGGCCA 1 cut(s) 978
Mph1103I ATGCAT 1 cut(s) 1624
MroXI GAANNNNTTC 2 cut(s) 257, 397
MscI TGGCCA 1 cut(s) 978
MseI TTAA 3 cut(s) 917, 1206, 1419
MslI CAYNNNNRTG 1 cut(s) 1130
Msp20I TGGCCA 1 cut(s) 978
MspCI CTTAAG 1 cut(s) 1418
MspR9I CCNGG 2 cut(s) 590, 1527
Mva1269I GAATGC 1 cut(s) 1480
MvaI CCWGG 2 cut(s) 590, 1527
MwoI GCNNNNNNNGC 3 cut(s) 14, 23, 883
NdeI CATATG 1 cut(s) 862
NdeII GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
NlaIII CATG 9 cut(s) 85, 215, 224, 526, 983, 1008, 1363, 1626, 1645
NlaIV GGNNCC 3 cut(s) 164, 898, 1509
NmuCI GTSAC 2 cut(s) 423, 911
NsiI ATGCAT 1 cut(s) 1624
NspI RCATGY 2 cut(s) 1363, 1626
OliI CACNNNNGTG 1 cut(s) 1130
PaeR7I CTCGAG 1 cut(s) 1053
PagI TCATGA 1 cut(s) 211
PceI AGGCCT 1 cut(s) 1355
PcsI WCGNNNNNNNCGW 1 cut(s) 118
PctI GAATGC 1 cut(s) 1480
PdmI GAANNNNTTC 2 cut(s) 257, 397
PfeI GAWTC 3 cut(s) 542, 1135, 1438
PfoI TCCNGGA 1 cut(s) 1525
PkrI GCNGC 4 cut(s) 25, 177, 226, 879
Psp6I CCWGG 2 cut(s) 588, 1525
PspGI CCWGG 2 cut(s) 588, 1525
PspN4I GGNNCC 3 cut(s) 164, 898, 1509
PspPI GGNCC 2 cut(s) 116, 293
PspXI VCTCGAGB 1 cut(s) 1053
PsrI GAACNNNNNNTAC 2 cut(s) 841, 873
PstNI CAGNNNCTG 2 cut(s) 23, 230
PsuI RGATCY 1 cut(s) 1283
RsaI GTAC 2 cut(s) 39, 582
RsaNI GTAC 2 cut(s) 38, 581
RseI CAYNNNNRTG 1 cut(s) 1130
SaqAI TTAA 3 cut(s) 917, 1206, 1419
SatI GCNGC 4 cut(s) 24, 176, 225, 878
Sau3AI GATC 8 cut(s) 86, 208, 217, 458, 619, 1273, 1283, 1522
Sau96I GGNCC 2 cut(s) 116, 293
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 2 cut(s) 590, 1527
SduI GDGCHC 2 cut(s) 1054, 1125
SfaNI GCATC 4 cut(s) 481, 705, 1084, 1279
Sfr274I CTCGAG 1 cut(s) 1053
SinI GGWCC 1 cut(s) 293
SlaI CTCGAG 1 cut(s) 1053
SmiMI CAYNNNNRTG 1 cut(s) 1130
SmlI CTYRAG 3 cut(s) 1053, 1418, 1565
SmoI CTYRAG 3 cut(s) 1053, 1418, 1565
Sse9I AATT 7 cut(s) 108, 363, 483, 673, 705, 1202, 1326
SseBI AGGCCT 1 cut(s) 1355
SsiI CCGC 2 cut(s) 402, 1160
SspI AATATT 2 cut(s) 300, 696
SspMI CTAG 3 cut(s) 383, 1022, 1091
StuI AGGCCT 1 cut(s) 1355
StyD4I CCNGG 2 cut(s) 588, 1525
StyI CCWWGG 2 cut(s) 387, 411
TaaI ACNGT 1 cut(s) 42
TaiI ACGT 2 cut(s) 124, 850
TaqI TCGA 4 cut(s) 112, 452, 1054, 1108
TasI AATT 7 cut(s) 108, 363, 483, 673, 705, 1202, 1326
TatI WGTACW 2 cut(s) 37, 580
TfiI GAWTC 3 cut(s) 542, 1135, 1438
Tru1I TTAA 3 cut(s) 917, 1206, 1419
Tru9I TTAA 3 cut(s) 917, 1206, 1419
TscAI CASTG 2 cut(s) 828, 994
TseFI GTSAC 2 cut(s) 423, 911
TseI GCWGC 4 cut(s) 23, 175, 224, 877
Tsp45I GTSAC 2 cut(s) 423, 911
TspDTI ATGAA 9 cut(s) 300, 300, 542, 591, 656, 849, 867, 1130, 1587
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 2 cut(s) 828, 994
Vha464I CTTAAG 1 cut(s) 1418
VpaK11BI GGWCC 1 cut(s) 293
XagI CCTNNNNNAGG 1 cut(s) 509
XapI RAATTY 2 cut(s) 108, 1202
XbaI TCTAGA 1 cut(s) 1090
XceI RCATGY 2 cut(s) 1363, 1626
XhoI CTCGAG 1 cut(s) 1053
XmnI GAANNNNTTC 2 cut(s) 257, 397
XspI CTAG 3 cut(s) 383, 1022, 1091
ZrmI AGTACT 1 cut(s) 39
Zsp2I ATGCAT 1 cut(s) 1624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.