Rh1BG160200

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001937
Physical Location & Seq
Forward (+)
25890994 .. 25896273
5280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG160200.1

Sequence Viewer

Length: 1209 bp
ATGGAAGTGGTAAAGGAAATTAGCTTGTGTAAGTCTCTAGTCTTGGGAAATCCTTCTGTAACATCCAAGGTTCTTGGGTCACTATTGGGAGAGGGCATTTTAGCGTCGAACGGATCAATGTGGGAGCATCAAAGGAAATTTATCGCTCCTGAACTATACTTTGATAAGGTGAAGGGCATGATGAACCTGGTAGTGGATTCAACAACCACAATGCTAAGAACTTGGGAAAATGAAGTCCAAAGCCAGGGAGAACTTGCTGAAATTAGAGTTGATGATCATTTCAGAAACTTATCAGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGCTGCAAAAAGTTTTGGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACAGAAAATAGAAAGATATGGATGTTGGAGAAGGAAACTCAATCACTGATATTGAAGGTGGTAAAACAACGTAGTGAAGGTTCATATGAGAAAGATTTGCTGCAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGACTTAAATGGCCTATCACACGATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCAGTGCCCTTGTAGCATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTACTTGAAATCTGTGGTGATAAGCCTCTAGATGCAGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGTACTCTTTGTAGCAAGAGAGGTATTTGAAACTATCAAATTTAAAGACATTACAATTCCAAAGGGGACGCTACTTCAAATACCAATCCCCTTCTTACACCAGAACCCTCATCTATGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTGCCAATGGAATTGTCAAGGCTTGCAAGTCTCCACAGGCCTACATGCCATTCGGAGCAGGTTCTCGTATATGTGTTGGTCAACAGTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATGATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGCATACCAGCATTCCCCTGCGTATAACATTATTCTGGAGCCTGAATATGGTATTATTCTACAAATGCGAAGAGCGTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

402

Amino Acids

45.4

Weight (kDa)

8.68

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 23 - 384 1e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1019
AccB1I GGYRCC 1 cut(s) 551
AciI CCGC 1 cut(s) 815
AclWI GGATC 2 cut(s) 121, 946
AcoI YGGCCR 1 cut(s) 631
AcsI RAATTY 2 cut(s) 137, 857
AfaI GTAC 2 cut(s) 720, 822
AfiI CCNNNNNNNGG 3 cut(s) 193, 244, 1172
AflII CTTAAG 1 cut(s) 1073
AgsI TTSAA 7 cut(s) 201, 487, 548, 725, 848, 896, 958
AjnI CCWGG 2 cut(s) 186, 243
AleI CACNNNNGTG 1 cut(s) 785
AluBI AGCT 7 cut(s) 24, 314, 370, 666, 680, 701, 713
AluI AGCT 7 cut(s) 24, 314, 370, 666, 680, 701, 713
Alw21I GWGCWC 2 cut(s) 709, 780
Alw26I GTCTC 3 cut(s) 39, 632, 1005
AlwI GGATC 2 cut(s) 121, 946
Ama87I CYCGRG 1 cut(s) 708
AoxI GGCC 3 cut(s) 577, 631, 1008
ApeKI GCWGC 2 cut(s) 370, 532
ApoI RAATTY 2 cut(s) 137, 857
Asp700I GAANNNNTTC 1 cut(s) 52
AsuHPI GGTGA 4 cut(s) 181, 578, 746, 799
AvaI CYCGRG 1 cut(s) 708
BaeGI GKGCMC 1 cut(s) 649
BalI TGGCCA 1 cut(s) 633
BanI GGYRCC 1 cut(s) 551
BauI CACGAG 1 cut(s) 702
Bbv12I GWGCWC 2 cut(s) 709, 780
BbvCI CCTCAGC 1 cut(s) 714
BbvI GCAGC 2 cut(s) 357, 519
BccI CCATC 3 cut(s) 391, 414, 693
BciT130I CCWGG 2 cut(s) 188, 245
BclI TGATCA 1 cut(s) 274
BcoDI GTCTC 3 cut(s) 39, 632, 1005
BfaI CTAG 3 cut(s) 38, 677, 746
BfrI CTTAAG 1 cut(s) 1073
BfuAI ACCTGC 1 cut(s) 1019
BisI GCNGC 2 cut(s) 371, 533
BlsI GCNGC 2 cut(s) 372, 534
Bme1390I CCNGG 2 cut(s) 188, 245
BmeT110I CYCGRG 1 cut(s) 708
BmiI GGNNCC 2 cut(s) 553, 1164
BmrFI CCNGG 2 cut(s) 188, 245
BmsI GCATC 4 cut(s) 136, 665, 739, 934
BpmI CTGGAG 1 cut(s) 1181
Bpu10I CCTNAGC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 2 cut(s) 66, 244
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 244, 1172
Bse1I ACTGG 1 cut(s) 642
BseBI CCWGG 2 cut(s) 188, 245
BseDI CCNNGG 2 cut(s) 66, 244
BseGI GGATG 3 cut(s) 62, 459, 685
BseLI CCNNNNNNNGG 3 cut(s) 193, 244, 1172
BseMII CTCAG 1 cut(s) 705
BseNI ACTGG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 2 cut(s) 357, 519
BshFI GGCC 3 cut(s) 579, 633, 1010
BshNI GGYRCC 1 cut(s) 551
BsiHKAI GWGCWC 2 cut(s) 709, 780
BsiHKCI CYCGRG 1 cut(s) 708
BslFI GGGAC 1 cut(s) 898
BslI CCNNNNNNNGG 3 cut(s) 193, 244, 1172
BsmAI GTCTC 3 cut(s) 39, 632, 1005
BsmFI GGGAC 1 cut(s) 898
BsmI GAATGC 1 cut(s) 1135
BsnI GGCC 3 cut(s) 579, 633, 1010
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 1 cut(s) 708
Bsp1286I GDGCHC 3 cut(s) 649, 709, 780
Bsp143I GATC 3 cut(s) 113, 274, 938
BspACI CCGC 1 cut(s) 815
BspANI GGCC 3 cut(s) 579, 633, 1010
BspCNI CTCAG 1 cut(s) 706
BspLI GGNNCC 2 cut(s) 553, 1164
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 2 cut(s) 121, 946
BspQI GCTCTTC 1 cut(s) 1189
BspT107I GGYRCC 1 cut(s) 551
BspTI CTTAAG 1 cut(s) 1073
BspTNI GGTCTC 1 cut(s) 632
BsrI ACTGG 1 cut(s) 642
BssECI CCNNGG 2 cut(s) 66, 244
BssMI GATC 3 cut(s) 113, 274, 938
BssSI CACGAG 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 66
Bst2BI CACGAG 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 188, 245
Bst4CI ACNGT 1 cut(s) 1056
Bst6I CTCTTC 1 cut(s) 1189
BstAFI CTTAAG 1 cut(s) 1073
BstC8I GCNNGC 4 cut(s) 631, 668, 699, 994
BstDEI CTNAG 2 cut(s) 215, 714
BstF5I GGATG 3 cut(s) 62, 459, 685
BstKTI GATC 3 cut(s) 116, 277, 941
BstMAI GTCTC 3 cut(s) 39, 632, 1005
BstMBI GATC 3 cut(s) 113, 274, 938
BstMWI GCNNNNNNNGC 2 cut(s) 538, 653
BstNI CCWGG 2 cut(s) 188, 245
BstNSI RCATGY 1 cut(s) 1018
BstSCI CCNGG 2 cut(s) 186, 243
BstSLI GKGCMC 1 cut(s) 649
BstV1I GCAGC 2 cut(s) 357, 519
BstX2I RGATCY 1 cut(s) 938
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 3 cut(s) 579, 633, 1010
BtsCI GGATG 3 cut(s) 62, 459, 685
BtsIMutI CAGTG 2 cut(s) 476, 649
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 4 cut(s) 631, 668, 699, 994
CseI GACGC 4 cut(s) 93, 794, 895, 1188
CsiI ACCWGGT 1 cut(s) 186
Csp6I GTAC 2 cut(s) 719, 821
CviAII CATG 4 cut(s) 178, 635, 660, 1015
CviQI GTAC 2 cut(s) 719, 821
DdeI CTNAG 2 cut(s) 215, 714
DpnI GATC 3 cut(s) 115, 276, 940
DpnII GATC 3 cut(s) 113, 274, 938
DraI TTTAAA 1 cut(s) 862
EaeI YGGCCR 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 1189
EarI CTCTTC 1 cut(s) 1189
Eco130I CCWWGG 1 cut(s) 66
Eco147I AGGCCT 1 cut(s) 1010
Eco31I GGTCTC 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 708
EcoRII CCWGG 2 cut(s) 186, 243
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 4 cut(s) 181, 638, 663, 1018
FalI AAGNNNNNCTT 2 cut(s) 1094, 1126
FaqI GGGAC 1 cut(s) 898
FatI CATG 4 cut(s) 177, 634, 659, 1014
FauI CCCGC 1 cut(s) 822
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 371, 533
FokI GGATG 3 cut(s) 49, 466, 672
Fsp4HI GCNGC 2 cut(s) 371, 533
FspBI CTAG 3 cut(s) 38, 677, 746
GluI GCNGC 2 cut(s) 371, 533
GsuI CTGGAG 1 cut(s) 1181
HaeIII GGCC 3 cut(s) 579, 633, 1010
HgaI GACGC 4 cut(s) 93, 794, 895, 1188
Hin1II CATG 4 cut(s) 181, 638, 663, 1018
HincII GTYRAC 1 cut(s) 1052
HindII GTYRAC 1 cut(s) 1052
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 3 cut(s) 197, 790, 1093
HphI GGTGA 4 cut(s) 181, 578, 746, 799
Hpy166II GTNNAC 1 cut(s) 1052
Hpy188I TCNGA 4 cut(s) 284, 429, 943, 1025
Hpy188III TCNNGA 6 cut(s) 149, 545, 689, 746, 794, 1160
Hpy8I GTNNAC 1 cut(s) 1052
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 7 cut(s) 63, 166, 457, 481, 503, 542, 919
HpyCH4III ACNGT 1 cut(s) 1056
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 5 cut(s) 373, 535, 612, 752, 996
HpyF10VI GCNNNNNNNGC 2 cut(s) 538, 653
HpyF3I CTNAG 2 cut(s) 215, 714
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 4 cut(s) 181, 638, 663, 1018
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 3 cut(s) 113, 274, 938
LguI GCTCTTC 1 cut(s) 1189
LmnI GCTCC 5 cut(s) 124, 151, 663, 1025, 1162
Lsp1109I GCAGC 2 cut(s) 357, 519
LweI GCATC 4 cut(s) 136, 665, 739, 934
MabI ACCWGGT 1 cut(s) 186
MaeI CTAG 3 cut(s) 38, 677, 746
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 4 cut(s) 58, 78, 566, 672
MalI GATC 3 cut(s) 115, 276, 940
MboI GATC 3 cut(s) 113, 274, 938
MboII GAAGA 1 cut(s) 1206
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 3 cut(s) 649, 709, 780
MlsI TGGCCA 1 cut(s) 633
MluCI AATT 8 cut(s) 18, 137, 261, 328, 360, 857, 873, 981
MluNI TGGCCA 1 cut(s) 633
MmeI TCCRAC 2 cut(s) 438, 452
MnlI CCTC 7 cut(s) 85, 709, 753, 790, 832, 936, 1127
Mox20I TGGCCA 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 52
MscI TGGCCA 1 cut(s) 633
MseI TTAA 3 cut(s) 572, 861, 1074
MslI CAYNNNNRTG 1 cut(s) 785
Msp20I TGGCCA 1 cut(s) 633
MspCI CTTAAG 1 cut(s) 1073
MspR9I CCNGG 2 cut(s) 188, 245
Mva1269I GAATGC 1 cut(s) 1135
MvaI CCWGG 2 cut(s) 188, 245
MwoI GCNNNNNNNGC 2 cut(s) 538, 653
NdeI CATATG 1 cut(s) 517
NdeII GATC 3 cut(s) 113, 274, 938
NlaIII CATG 4 cut(s) 181, 638, 663, 1018
NlaIV GGNNCC 2 cut(s) 553, 1164
NmuCI GTSAC 2 cut(s) 78, 566
NspI RCATGY 1 cut(s) 1018
OliI CACNNNNGTG 1 cut(s) 785
PaeR7I CTCGAG 1 cut(s) 708
PceI AGGCCT 1 cut(s) 1010
PciSI GCTCTTC 1 cut(s) 1189
PctI GAATGC 1 cut(s) 1135
PdmI GAANNNNTTC 1 cut(s) 52
PfeI GAWTC 3 cut(s) 197, 790, 1093
PkrI GCNGC 2 cut(s) 372, 534
Psp6I CCWGG 2 cut(s) 186, 243
PspGI CCWGG 2 cut(s) 186, 243
PspN4I GGNNCC 2 cut(s) 553, 1164
PspXI VCTCGAGB 1 cut(s) 708
PsrI GAACNNNNNNTAC 2 cut(s) 496, 528
PsuI RGATCY 1 cut(s) 938
RsaI GTAC 2 cut(s) 720, 822
RsaNI GTAC 2 cut(s) 719, 821
RseI CAYNNNNRTG 1 cut(s) 785
SapI GCTCTTC 1 cut(s) 1189
SaqAI TTAA 3 cut(s) 572, 861, 1074
SatI GCNGC 2 cut(s) 371, 533
Sau3AI GATC 3 cut(s) 113, 274, 938
ScrFI CCNGG 2 cut(s) 188, 245
SduI GDGCHC 3 cut(s) 649, 709, 780
SexAI ACCWGGT 1 cut(s) 186
SfaNI GCATC 4 cut(s) 136, 665, 739, 934
Sfr274I CTCGAG 1 cut(s) 708
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 1 cut(s) 785
SmlI CTYRAG 2 cut(s) 708, 1073
SmoI CTYRAG 2 cut(s) 708, 1073
Sse9I AATT 8 cut(s) 18, 137, 261, 328, 360, 857, 873, 981
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 1 cut(s) 815
SspI AATATT 1 cut(s) 351
SspMI CTAG 3 cut(s) 38, 677, 746
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 2 cut(s) 186, 243
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 1056
TaiI ACGT 1 cut(s) 505
TaqI TCGA 3 cut(s) 107, 709, 763
TasI AATT 8 cut(s) 18, 137, 261, 328, 360, 857, 873, 981
TatI WGTACW 1 cut(s) 820
TfiI GAWTC 3 cut(s) 197, 790, 1093
Tru1I TTAA 3 cut(s) 572, 861, 1074
Tru9I TTAA 3 cut(s) 572, 861, 1074
TscAI CASTG 2 cut(s) 483, 649
TseFI GTSAC 2 cut(s) 78, 566
TseI GCWGC 2 cut(s) 370, 532
Tsp45I GTSAC 2 cut(s) 78, 566
TspDTI ATGAA 5 cut(s) 197, 246, 311, 504, 785
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 2 cut(s) 483, 649
Vha464I CTTAAG 1 cut(s) 1073
XapI RAATTY 2 cut(s) 137, 857
XbaI TCTAGA 1 cut(s) 745
XceI RCATGY 1 cut(s) 1018
XhoI CTCGAG 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 52
XspI CTAG 3 cut(s) 38, 677, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.