Rw1G015960

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001937
Physical Location & Seq
Forward (+)
35589989 .. 35593766
3778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015960.1

Sequence Viewer

Length: 1590 bp
ATGGAAGCAGAGCTATTAGTGGTAGCAGCAAAGATCACATTGTCGGTATTGGTGGGTGGGTTTCTGCTGTTGTTCTTGTATCTCTATGAACTACTAATATTGAGGCCTAAAGGGCTAAGATCAAAGCTCCAAAAGCAAGGAATTAGAGGGCCTCGTCCGTCATTTCTCTTGGGGAATATCCTTGCATTAAAGAGCCTTCAAAGCAAGGTCCAGTCATCTGCATCAAAGACTACAGATCCTCAAAAGCCCTATCATGATGATGATCGTGTGGCTTCTATTTGTCATCAATGGCCTTCAAAAATCTTCCCCCACATAGAGGAATGGAGAAATGAATATGGGCCAACATTTATGTATGCACTTGTCCACATGCAACAATTATGTATAACAGATGTAGAGCTATTGAAGGAACTTGGGCTTTGCAAGTCATTAATCTTGGGCACTCCTTCCATAACGTCCAATATTCTCGGACCTCTTCTGGGACAGGGTCTTTTATCATCGAATGGATTAATTTGGGCCCATCAGAGGAAAACTATCGCTCCTGAATTATACCTTGCTAAGATCAAGGGCATGATGGACCTAATAGTGGACTCTACAACCTCAGTGCTAAGAACTTGGGAAAGTGAAATCCAAACGCAGGGAGGAACTGCAGAAATTAGAATTGATGAGAATCTAAGAAACATATCTGGAGATATAATTGCAAAAGCTTGTTTTCAGAGCAATTATAGTGAAGGAGAAGAAATATTCTCAAGACTTAGGACTCTACAAAAGGTTTTGTCAAAGGGTATGATGGGGGTAGAAGGCTTAATCAGATGGTTGCCAACAATCGAAAATAGAGAAATATGGAGATTAGAGAAGGAAATTGAGTCAATGATATTGAAGGTGGTAAAACAGCGCAGTGAAGCTTCATATGAGAAAGATTTGCTGCAAATGATTCTTCAGGGTGCTGAAACTTCCGGTGAAAATGGCCTATCACATAAGAAGTTCATTGTTGATAATTGCAAAACCATATTCATTGCTGCCCATGAGACCACTGCCGTTGCAGCATCATGGAGCTTGATGCTACTAGCTTTACATCCAGATTGGCAAGCTCGTGTTCGTGCTGAGGTGCTCGAAATCTGCGGGGATAGGCCTCTACATGCAGATATGCTTCCAAGCATGAAAGTGCTCACAATATTGATTCAGGAAGTATTGCGTCTATACTCGCCTGTATTTTTTTTAGCAAGAGAGGTCCTTGAAACTATCACATTGAAAAACATTGTGATACCAAAGGGTGTGGTCCTTCAGATACCAGTACCCTTTCTACACCAGAACCCTGATATATGGGGCCCTGACGCGCATAAGTTCAATCCAGAAAGATTTGCCAGTGGAGTTCTCAAGGCTTGCAACTCTCCACATGCCTACATGCCATTCGGACTTGGTCCCCGAATCTGTGTTGGTCAACATTTAGCAATAACTGAGCTTAAGGTGATTCTTTCTTTGATTCTATCAAAGTTTTGTTTTTCCCTCTCACCAGCATACCAGCATTCCCCTGCTTATAATCTCTTAACGGAGCCTGAACATGGTGTCATTCTCCGTATGAGGAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

529

Amino Acids

59.4

Weight (kDa)

8.44

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 102 - 513 3.7e-75 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AccII CGCG 1 cut(s) 1334
AciI CCGC 1 cut(s) 1119
AclWI GGATC 1 cut(s) 230
AcuI CTGAAG 2 cut(s) 920, 1265
AfaI GTAC 1 cut(s) 1293
AfiI CCNNNNNNNGG 6 cut(s) 316, 476, 522, 583, 634, 1559
AflII CTTAAG 1 cut(s) 1460
AgsI TTSAA 7 cut(s) 200, 297, 403, 877, 1235, 1249, 1345
AluBI AGCT 9 cut(s) 13, 127, 397, 704, 902, 1053, 1067, 1088, 1459
AluI AGCT 9 cut(s) 13, 127, 397, 704, 902, 1053, 1067, 1088, 1459
Alw21I GWGCWC 2 cut(s) 1110, 1167
Alw26I GTCTC 1 cut(s) 1019
AlwI GGATC 1 cut(s) 230
AoxI GGCC 8 cut(s) 104, 149, 290, 338, 513, 964, 1127, 1324
ApaI GGGCCC 2 cut(s) 517, 1328
ApeKI GCWGC 4 cut(s) 26, 922, 1016, 1040
AseI ATTAAT 2 cut(s) 428, 506
AspLEI GCGC 2 cut(s) 894, 1336
AsuHPI GGTGA 3 cut(s) 968, 1477, 1500
AvaII GGWCC 6 cut(s) 208, 467, 574, 1228, 1276, 1418
BaeGI GKGCMC 3 cut(s) 440, 517, 1328
BanII GRGCYC 2 cut(s) 517, 1328
BauI CACGAG 1 cut(s) 1089
Bbv12I GWGCWC 2 cut(s) 1110, 1167
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 4 cut(s) 38, 909, 1003, 1052
BccI CCATC 4 cut(s) 525, 565, 781, 804
BceAI ACGGC 1 cut(s) 1019
BcoDI GTCTC 1 cut(s) 1019
BfaI CTAG 1 cut(s) 1064
BfmI CTRYAG 2 cut(s) 231, 645
BfrI CTTAAG 1 cut(s) 1460
BglI GCCNNNNNGGC 1 cut(s) 112
BisI GCNGC 4 cut(s) 27, 923, 1017, 1041
BlsI GCNGC 4 cut(s) 28, 924, 1018, 1042
Bme18I GGWCC 6 cut(s) 208, 467, 574, 1228, 1276, 1418
BmiI GGNNCC 5 cut(s) 515, 1325, 1326, 1420, 1551
BmsI GCATC 3 cut(s) 230, 1047, 1052
BpmI CTGGAG 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 1101
BpuEI CTTGAG 2 cut(s) 730, 1358
BsaBI GATNNNNATC 2 cut(s) 261, 666
BsaI GGTCTC 1 cut(s) 1019
BsaWI WCCGGW 1 cut(s) 953
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bsc4I CCNNNNNNNGG 6 cut(s) 316, 476, 522, 583, 634, 1559
Bse1I ACTGG 3 cut(s) 211, 1289, 1362
Bse3DI GCAATG 1 cut(s) 1011
Bse8I GATNNNNATC 2 cut(s) 261, 666
BseGI GGATG 1 cut(s) 1072
BseJI GATNNNNATC 2 cut(s) 261, 666
BseLI CCNNNNNNNGG 6 cut(s) 316, 476, 522, 583, 634, 1559
BseMI GCAATG 1 cut(s) 1011
BseMII CTCAG 3 cut(s) 612, 1092, 1446
BseNI ACTGG 3 cut(s) 211, 1289, 1362
BseSI GKGCMC 3 cut(s) 440, 517, 1328
BseXI GCAGC 4 cut(s) 38, 909, 1003, 1052
Bsh1236I CGCG 1 cut(s) 1334
BshFI GGCC 8 cut(s) 106, 151, 292, 340, 515, 966, 1129, 1326
BsiHKAI GWGCWC 2 cut(s) 1110, 1167
BsiSI CCGG 1 cut(s) 954
BslFI GGGAC 2 cut(s) 492, 1404
BslI CCNNNNNNNGG 6 cut(s) 316, 476, 522, 583, 634, 1559
BsmAI GTCTC 1 cut(s) 1019
BsmFI GGGAC 2 cut(s) 492, 1404
BsmI GAATGC 1 cut(s) 1522
BsnI GGCC 8 cut(s) 106, 151, 292, 340, 515, 966, 1129, 1326
Bso31I GGTCTC 1 cut(s) 1019
Bsp120I GGGCCC 2 cut(s) 513, 1324
Bsp1286I GDGCHC 5 cut(s) 440, 517, 1110, 1167, 1328
Bsp143I GATC 5 cut(s) 33, 119, 235, 262, 558
BspACI CCGC 1 cut(s) 1119
BspANI GGCC 8 cut(s) 106, 151, 292, 340, 515, 966, 1129, 1326
BspCNI CTCAG 3 cut(s) 611, 1093, 1447
BspFNI CGCG 1 cut(s) 1334
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 5 cut(s) 515, 1325, 1326, 1420, 1551
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 1 cut(s) 230
BspTI CTTAAG 1 cut(s) 1460
BspTNI GGTCTC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 1011
BsrI ACTGG 3 cut(s) 211, 1289, 1362
BssMI GATC 5 cut(s) 33, 119, 235, 262, 558
BssSI CACGAG 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 1089
Bst6I CTCTTC 1 cut(s) 477
BstAFI CTTAAG 1 cut(s) 1460
BstC8I GCNNGC 2 cut(s) 1086, 1381
BstDEI CTNAG 8 cut(s) 116, 555, 598, 605, 671, 752, 1101, 1455
BstF5I GGATG 1 cut(s) 1072
BstFNI CGCG 1 cut(s) 1334
BstHHI GCGC 2 cut(s) 894, 1336
BstKTI GATC 5 cut(s) 36, 122, 238, 265, 561
BstMAI GTCTC 1 cut(s) 1019
BstMBI GATC 5 cut(s) 33, 119, 235, 262, 558
BstMWI GCNNNNNNNGC 4 cut(s) 112, 133, 201, 1040
BstNSI RCATGY 4 cut(s) 370, 1139, 1397, 1405
BstSFI CTRYAG 2 cut(s) 231, 645
BstSLI GKGCMC 3 cut(s) 440, 517, 1328
BstUI CGCG 1 cut(s) 1334
BstV1I GCAGC 4 cut(s) 38, 909, 1003, 1052
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuRI GGCC 8 cut(s) 106, 151, 292, 340, 515, 966, 1129, 1326
BtsCI GGATG 1 cut(s) 1072
BtsI GCAGTG 2 cut(s) 901, 1029
BtsIMutI CAGTG 4 cut(s) 606, 901, 1029, 1369
Cac8I GCNNGC 2 cut(s) 1086, 1381
CciI TCATGA 1 cut(s) 253
CfoI GCGC 2 cut(s) 894, 1336
CseI GACGC 2 cut(s) 1181, 1340
Csp6I GTAC 1 cut(s) 1292
CspCI CAANNNNNGTGG 4 cut(s) 1018, 1053, 1254, 1289
CviQI GTAC 1 cut(s) 1292
DdeI CTNAG 8 cut(s) 116, 555, 598, 605, 671, 752, 1101, 1455
DpnI GATC 5 cut(s) 35, 121, 237, 264, 560
DpnII GATC 5 cut(s) 33, 119, 235, 262, 558
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco147I AGGCCT 2 cut(s) 106, 1129
Eco24I GRGCYC 2 cut(s) 517, 1328
Eco31I GGTCTC 1 cut(s) 1019
Eco47I GGWCC 6 cut(s) 208, 467, 574, 1228, 1276, 1418
Eco57I CTGAAG 2 cut(s) 920, 1265
EcoO109I RGGNCCY 4 cut(s) 149, 1228, 1324, 1325
EcoT38I GRGCYC 2 cut(s) 517, 1328
FaqI GGGAC 2 cut(s) 492, 1404
FauI CCCGC 1 cut(s) 1112
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 4 cut(s) 27, 923, 1017, 1041
FokI GGATG 1 cut(s) 1059
FriOI GRGCYC 2 cut(s) 517, 1328
Fsp4HI GCNGC 4 cut(s) 27, 923, 1017, 1041
FspBI CTAG 1 cut(s) 1064
GlaI GCGC 2 cut(s) 893, 1335
GluI GCNGC 4 cut(s) 27, 923, 1017, 1041
GsuI CTGGAG 1 cut(s) 705
HaeIII GGCC 8 cut(s) 106, 151, 292, 340, 515, 966, 1129, 1326
HapII CCGG 1 cut(s) 954
HgaI GACGC 2 cut(s) 1181, 1340
HhaI GCGC 2 cut(s) 894, 1336
Hin6I GCGC 2 cut(s) 892, 1334
HinP1I GCGC 2 cut(s) 892, 1334
HincII GTYRAC 1 cut(s) 1439
HindII GTYRAC 1 cut(s) 1439
HindIII AAGCTT 2 cut(s) 702, 900
HinfI GANTC 9 cut(s) 587, 667, 757, 863, 931, 1177, 1425, 1468, 1480
HpaII CCGG 1 cut(s) 954
HphI GGTGA 3 cut(s) 968, 1477, 1500
Hpy166II GTNNAC 3 cut(s) 364, 586, 1439
Hpy188I TCNGA 6 cut(s) 467, 522, 714, 809, 1284, 1412
Hpy188III TCNNGA 7 cut(s) 254, 539, 684, 747, 1076, 1181, 1349
Hpy8I GTNNAC 3 cut(s) 364, 586, 1439
HpyAV CCTTC 9 cut(s) 206, 303, 397, 453, 722, 791, 847, 871, 1289
HpyCH4IV ACGT 1 cut(s) 452
HpyF10VI GCNNNNNNNGC 4 cut(s) 112, 133, 201, 1040
HpyF3I CTNAG 8 cut(s) 116, 555, 598, 605, 671, 752, 1101, 1455
HpySE526I ACGT 1 cut(s) 452
HspAI GCGC 2 cut(s) 892, 1334
Kzo9I GATC 5 cut(s) 33, 119, 235, 262, 558
LmnI GCTCC 4 cut(s) 132, 541, 1050, 1549
Lsp1109I GCAGC 4 cut(s) 38, 909, 1003, 1052
LweI GCATC 3 cut(s) 230, 1047, 1052
MaeI CTAG 1 cut(s) 1064
MaeII ACGT 1 cut(s) 452
MalI GATC 5 cut(s) 35, 121, 237, 264, 560
MboI GATC 5 cut(s) 33, 119, 235, 262, 558
MboII GAAGA 4 cut(s) 295, 464, 746, 926
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 5 cut(s) 440, 517, 1110, 1167, 1328
MlyI GAGTC 3 cut(s) 581, 751, 872
MseI TTAA 6 cut(s) 188, 428, 506, 803, 1461, 1544
MslI CAYNNNNRTG 2 cut(s) 258, 1160
MspCI CTTAAG 1 cut(s) 1460
MspI CCGG 1 cut(s) 954
Mva1269I GAATGC 1 cut(s) 1522
MvnI CGCG 1 cut(s) 1334
MwoI GCNNNNNNNGC 4 cut(s) 112, 133, 201, 1040
NdeI CATATG 1 cut(s) 907
NdeII GATC 5 cut(s) 33, 119, 235, 262, 558
NlaIV GGNNCC 5 cut(s) 515, 1325, 1326, 1420, 1551
NspI RCATGY 4 cut(s) 370, 1139, 1397, 1405
PagI TCATGA 1 cut(s) 253
PceI AGGCCT 2 cut(s) 106, 1129
PctI GAATGC 1 cut(s) 1522
PfeI GAWTC 6 cut(s) 667, 931, 1177, 1425, 1468, 1480
PflFI GACNNNGTC 2 cut(s) 483, 1416
PkrI GCNGC 4 cut(s) 28, 924, 1018, 1042
PleI GAGTC 3 cut(s) 581, 751, 871
PpsI GAGTC 3 cut(s) 581, 751, 871
PpuMI RGGWCCY 1 cut(s) 1228
PshBI ATTAAT 2 cut(s) 428, 506
PsiI TTATAA 1 cut(s) 1536
Psp5II RGGWCCY 1 cut(s) 1228
PspN4I GGNNCC 5 cut(s) 515, 1325, 1326, 1420, 1551
PspOMI GGGCCC 2 cut(s) 513, 1324
PspPPI RGGWCCY 1 cut(s) 1228
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 2 cut(s) 483, 1416
RsaI GTAC 1 cut(s) 1293
RsaNI GTAC 1 cut(s) 1292
RseI CAYNNNNRTG 2 cut(s) 258, 1160
SaqAI TTAA 6 cut(s) 188, 428, 506, 803, 1461, 1544
SatI GCNGC 4 cut(s) 27, 923, 1017, 1041
Sau3AI GATC 5 cut(s) 33, 119, 235, 262, 558
SchI GAGTC 3 cut(s) 581, 751, 872
SduI GDGCHC 5 cut(s) 440, 517, 1110, 1167, 1328
SfaNI GCATC 3 cut(s) 230, 1047, 1052
SfcI CTRYAG 2 cut(s) 231, 645
SinI GGWCC 6 cut(s) 208, 467, 574, 1228, 1276, 1418
SmiMI CAYNNNNRTG 2 cut(s) 258, 1160
SmlI CTYRAG 3 cut(s) 745, 1373, 1460
SmoI CTYRAG 3 cut(s) 745, 1373, 1460
SseBI AGGCCT 2 cut(s) 106, 1129
SsiI CCGC 1 cut(s) 1119
SspI AATATT 4 cut(s) 99, 460, 741, 1173
SspMI CTAG 1 cut(s) 1064
StuI AGGCCT 2 cut(s) 106, 1129
TaiI ACGT 1 cut(s) 455
TaqI TCGA 3 cut(s) 497, 825, 1110
TfiI GAWTC 6 cut(s) 667, 931, 1177, 1425, 1468, 1480
Tru1I TTAA 6 cut(s) 188, 428, 506, 803, 1461, 1544
Tru9I TTAA 6 cut(s) 188, 428, 506, 803, 1461, 1544
TscAI CASTG 4 cut(s) 606, 901, 1036, 1369
TseI GCWGC 4 cut(s) 26, 922, 1016, 1040
TspDTI ATGAA 6 cut(s) 102, 345, 894, 973, 1000, 1172
TspGWI ACGGA 3 cut(s) 147, 1562, 1562
TspRI CASTG 4 cut(s) 606, 901, 1036, 1369
Tth111I GACNNNGTC 2 cut(s) 483, 1416
Vha464I CTTAAG 1 cut(s) 1460
VpaK11BI GGWCC 6 cut(s) 208, 467, 574, 1228, 1276, 1418
VspI ATTAAT 2 cut(s) 428, 506
XceI RCATGY 4 cut(s) 370, 1139, 1397, 1405
XspI CTAG 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.