Rh1BG160300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001937
Physical Location & Seq
Forward (+)
25913397 .. 25917855
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG160300.1

Sequence Viewer

Length: 1194 bp
ATGTATGCACTTGTCCACATTTACAAGTCATTAATCTTGGGCACTCCTTCCATAACGTCCAAGATTCTCGGACCTCTTCTGGGACAGGGTCTTTTATCATCGAATGGATTAATTTGGGCCCATCAAAGGAAAACTATCGCTCCTGAATTATACCTTGCTAAGATCAAGGGCATGATGGACCTAATAGTGGACTCTACAACCTCAGTGCTAAGAACTTGGGAAAGTGAAATCCAAACGCAGGGAGGAACTGCAGAAATTAGAGTTGATGAGAATCTAAGAAACATAGCTGGAGATATAATTGCAAAAGCTTGCTTTCAGAGCAATTATAGTGAAGGAGAAGAAATATTCTCAAGACTTAGGACTCTACAAAAGGTTTTGTCAAAGGGTATGATGGGGGTAGAAGGCTTAATCAGATGGTTGCCAACAAACGAAAATAGAGAAATATGGAGATTAGAGAAGGAAATTGAGTCAATGATATTGAAGGTGGTAAAACAGCGCAGTGAAGCTTCATATGAGAAAGATTTGCTGCAAATGATTCTTCAGGGTACTGAAACTTCGGGTGAAAATGGCCTATCACGTAAGAAGTTCATTGTTGATAATTGCAAAACCATATTCATTGCTGCCCATGAGACCACTGCCGTTGCAGCATCATGGAGCTTGATGCTACTAGCTTTACATCCAGATTGGCAAGCTTGTGTTCGTGCTGAGGTGCTCGAAATCTGCGGGGATAGGCCTCTACATGCAGATATGCTTCCAAGCATGAAAGTGCTCACAATGGTGATTCAGGAAGTATTGCGTCTGTACTCGCCTGTATTTTTTCTAGCAAGAGAGGTCTTTGAAACTATCACATTGAAAAACATTGTGATACCAAAGGGTGTAGTCCTTCAGATACCAGTACCCTTTCTACACCAGAACCCTGATATATGGGGCCCTGACGCGCATAAGTTCAATCCTGAAAGATTTGCCAATGGAGTTCTCAAGGCTTGCAACTCTCCACATGCCTACATGCCATATGGACTTGGTCCCCGAATCTGTGTTGGTCAACATTTAGCAATAACAGAGCTTAAGGTGATTCTTTCTTTGATTCTATCAAAGTTTTGTTTTTCCCTCTCATCAGCATACCAGCATTCCCCTGCTTATAAGCTCTTAACGGAGCCTGAACATGGTGTCATTCTCCGTATGAGGAGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

397

Amino Acids

44.56

Weight (kDa)

8.37

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 21 - 380 1.8e-70 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
AccII CGCG 1 cut(s) 938
AciI CCGC 1 cut(s) 723
AcuI CTGAAG 2 cut(s) 524, 869
AfaI GTAC 3 cut(s) 547, 803, 897
AfiI CCNNNNNNNGG 5 cut(s) 80, 126, 187, 238, 1163
AflII CTTAAG 1 cut(s) 1064
AgsI TTSAA 4 cut(s) 481, 839, 853, 949
AleI CACNNNNGTG 1 cut(s) 776
AluBI AGCT 8 cut(s) 287, 308, 506, 657, 671, 692, 1063, 1144
AluI AGCT 8 cut(s) 287, 308, 506, 657, 671, 692, 1063, 1144
Alw21I GWGCWC 2 cut(s) 714, 771
Alw26I GTCTC 1 cut(s) 623
AoxI GGCC 4 cut(s) 117, 568, 731, 928
ApaI GGGCCC 2 cut(s) 121, 932
ApeKI GCWGC 3 cut(s) 526, 620, 644
AseI ATTAAT 2 cut(s) 32, 110
AspLEI GCGC 2 cut(s) 498, 940
AspS9I GGNCC 7 cut(s) 71, 117, 118, 178, 928, 929, 1022
AsuHPI GGTGA 3 cut(s) 572, 790, 1081
AvaII GGWCC 3 cut(s) 71, 178, 1022
BaeGI GKGCMC 3 cut(s) 44, 121, 932
BanII GRGCYC 2 cut(s) 121, 932
BarI GAAGNNNNNNTAC 2 cut(s) 538, 570
Bbv12I GWGCWC 2 cut(s) 714, 771
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 3 cut(s) 513, 607, 656
BccI CCATC 4 cut(s) 129, 169, 385, 408
BceAI ACGGC 1 cut(s) 623
BcoDI GTCTC 1 cut(s) 623
BfaI CTAG 2 cut(s) 668, 821
BfmI CTRYAG 1 cut(s) 249
BfrI CTTAAG 1 cut(s) 1064
BisI GCNGC 3 cut(s) 527, 621, 645
BlsI GCNGC 3 cut(s) 528, 622, 646
Bme18I GGWCC 3 cut(s) 71, 178, 1022
BmgT120I GGNCC 7 cut(s) 71, 117, 118, 178, 928, 929, 1022
BmiI GGNNCC 5 cut(s) 119, 929, 930, 1024, 1155
BmsI GCATC 2 cut(s) 651, 656
BpmI CTGGAG 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 334, 962
BsaAI YACGTR 1 cut(s) 578
BsaBI GATNNNNATC 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 623
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 126, 187, 238, 1163
Bse1I ACTGG 1 cut(s) 893
Bse3DI GCAATG 1 cut(s) 615
Bse8I GATNNNNATC 1 cut(s) 270
BseGI GGATG 1 cut(s) 676
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 5 cut(s) 80, 126, 187, 238, 1163
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 2 cut(s) 216, 696
BseNI ACTGG 1 cut(s) 893
BseSI GKGCMC 3 cut(s) 44, 121, 932
BseXI GCAGC 3 cut(s) 513, 607, 656
Bsh1236I CGCG 1 cut(s) 938
BshFI GGCC 4 cut(s) 119, 570, 733, 930
BsiHKAI GWGCWC 2 cut(s) 714, 771
BslFI GGGAC 2 cut(s) 96, 1008
BslI CCNNNNNNNGG 5 cut(s) 80, 126, 187, 238, 1163
BsmAI GTCTC 1 cut(s) 623
BsmFI GGGAC 2 cut(s) 96, 1008
BsmI GAATGC 1 cut(s) 1126
BsnI GGCC 4 cut(s) 119, 570, 733, 930
Bso31I GGTCTC 1 cut(s) 623
Bsp120I GGGCCC 2 cut(s) 117, 928
Bsp1286I GDGCHC 5 cut(s) 44, 121, 714, 771, 932
Bsp143I GATC 1 cut(s) 162
BspACI CCGC 1 cut(s) 723
BspANI GGCC 4 cut(s) 119, 570, 733, 930
BspCNI CTCAG 2 cut(s) 215, 697
BspFNI CGCG 1 cut(s) 938
BspLI GGNNCC 5 cut(s) 119, 929, 930, 1024, 1155
BspMAI CTGCAG 1 cut(s) 253
BspTI CTTAAG 1 cut(s) 1064
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 615
BsrI ACTGG 1 cut(s) 893
BssMI GATC 1 cut(s) 162
Bst6I CTCTTC 1 cut(s) 81
BstAFI CTTAAG 1 cut(s) 1064
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 3 cut(s) 310, 690, 985
BstDEI CTNAG 6 cut(s) 159, 202, 209, 275, 356, 705
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 1 cut(s) 938
BstHHI GCGC 2 cut(s) 498, 940
BstKTI GATC 1 cut(s) 165
BstMAI GTCTC 1 cut(s) 623
BstMBI GATC 1 cut(s) 162
BstMWI GCNNNNNNNGC 2 cut(s) 318, 644
BstNSI RCATGY 3 cut(s) 743, 1001, 1009
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 3 cut(s) 44, 121, 932
BstUI CGCG 1 cut(s) 938
BstV1I GCAGC 3 cut(s) 513, 607, 656
BsuRI GGCC 4 cut(s) 119, 570, 733, 930
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 2 cut(s) 505, 633
BtsIMutI CAGTG 3 cut(s) 210, 505, 633
Cac8I GCNNGC 3 cut(s) 310, 690, 985
CfoI GCGC 2 cut(s) 498, 940
Cfr13I GGNCC 7 cut(s) 71, 117, 118, 178, 928, 929, 1022
CseI GACGC 2 cut(s) 785, 944
Csp6I GTAC 3 cut(s) 546, 802, 896
CspCI CAANNNNNGTGG 2 cut(s) 622, 657
CviAII CATG 8 cut(s) 172, 626, 651, 740, 760, 998, 1006, 1163
CviQI GTAC 3 cut(s) 546, 802, 896
DdeI CTNAG 6 cut(s) 159, 202, 209, 275, 356, 705
DpnI GATC 1 cut(s) 164
DpnII GATC 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 733
Eco24I GRGCYC 2 cut(s) 121, 932
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 3 cut(s) 71, 178, 1022
Eco57I CTGAAG 2 cut(s) 524, 869
EcoO109I RGGNCCY 2 cut(s) 928, 929
EcoT38I GRGCYC 2 cut(s) 121, 932
FaeI CATG 8 cut(s) 175, 629, 654, 743, 763, 1001, 1009, 1166
FaqI GGGAC 2 cut(s) 96, 1008
FatI CATG 8 cut(s) 171, 625, 650, 739, 759, 997, 1005, 1162
FauI CCCGC 1 cut(s) 716
FauNDI CATATG 2 cut(s) 511, 1012
Fnu4HI GCNGC 3 cut(s) 527, 621, 645
FokI GGATG 1 cut(s) 663
FriOI GRGCYC 2 cut(s) 121, 932
Fsp4HI GCNGC 3 cut(s) 527, 621, 645
FspBI CTAG 2 cut(s) 668, 821
GlaI GCGC 2 cut(s) 497, 939
GluI GCNGC 3 cut(s) 527, 621, 645
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 4 cut(s) 119, 570, 733, 930
HgaI GACGC 2 cut(s) 785, 944
HhaI GCGC 2 cut(s) 498, 940
Hin1II CATG 8 cut(s) 175, 629, 654, 743, 763, 1001, 1009, 1166
Hin6I GCGC 2 cut(s) 496, 938
HinP1I GCGC 2 cut(s) 496, 938
HincII GTYRAC 1 cut(s) 1043
HindII GTYRAC 1 cut(s) 1043
HindIII AAGCTT 3 cut(s) 306, 504, 690
HphI GGTGA 3 cut(s) 572, 790, 1081
Hpy166II GTNNAC 3 cut(s) 16, 190, 1043
Hpy188I TCNGA 4 cut(s) 71, 318, 413, 888
Hpy188III TCNNGA 5 cut(s) 143, 351, 680, 785, 953
Hpy8I GTNNAC 3 cut(s) 16, 190, 1043
HpyAV CCTTC 6 cut(s) 57, 326, 395, 451, 475, 893
HpyCH4IV ACGT 2 cut(s) 56, 577
HpyCH4V TGCA 8 cut(s) 8, 251, 302, 529, 603, 644, 743, 987
HpyF10VI GCNNNNNNNGC 2 cut(s) 318, 644
HpyF3I CTNAG 6 cut(s) 159, 202, 209, 275, 356, 705
HpySE526I ACGT 2 cut(s) 56, 577
Hsp92II CATG 8 cut(s) 175, 629, 654, 743, 763, 1001, 1009, 1166
HspAI GCGC 2 cut(s) 496, 938
Kzo9I GATC 1 cut(s) 162
LmnI GCTCC 3 cut(s) 145, 654, 1153
Lsp1109I GCAGC 3 cut(s) 513, 607, 656
LweI GCATC 2 cut(s) 651, 656
MaeI CTAG 2 cut(s) 668, 821
MaeII ACGT 2 cut(s) 56, 577
MalI GATC 1 cut(s) 164
MboI GATC 1 cut(s) 162
MboII GAAGA 3 cut(s) 68, 350, 530
MhlI GDGCHC 5 cut(s) 44, 121, 714, 771, 932
MluCI AATT 7 cut(s) 111, 146, 255, 297, 322, 462, 598
MlyI GAGTC 3 cut(s) 185, 355, 476
MnlI CCTC 8 cut(s) 84, 211, 236, 700, 744, 823, 1118, 1176
MseI TTAA 5 cut(s) 32, 110, 407, 1065, 1148
MslI CAYNNNNRTG 2 cut(s) 764, 776
MspCI CTTAAG 1 cut(s) 1064
Mva1269I GAATGC 1 cut(s) 1126
MvnI CGCG 1 cut(s) 938
MwoI GCNNNNNNNGC 2 cut(s) 318, 644
NdeI CATATG 2 cut(s) 511, 1012
NdeII GATC 1 cut(s) 162
NlaIII CATG 8 cut(s) 175, 629, 654, 743, 763, 1001, 1009, 1166
NlaIV GGNNCC 5 cut(s) 119, 929, 930, 1024, 1155
NspI RCATGY 3 cut(s) 743, 1001, 1009
OliI CACNNNNGTG 1 cut(s) 776
PceI AGGCCT 1 cut(s) 733
PctI GAATGC 1 cut(s) 1126
PfeI GAWTC 7 cut(s) 64, 271, 535, 781, 1029, 1072, 1084
PflFI GACNNNGTC 2 cut(s) 87, 1020
PkrI GCNGC 3 cut(s) 528, 622, 646
PleI GAGTC 3 cut(s) 185, 355, 475
PpsI GAGTC 3 cut(s) 185, 355, 475
Ppu21I YACGTR 1 cut(s) 578
PshBI ATTAAT 2 cut(s) 32, 110
PsiI TTATAA 1 cut(s) 1140
PspN4I GGNNCC 5 cut(s) 119, 929, 930, 1024, 1155
PspOMI GGGCCC 2 cut(s) 117, 928
PspPI GGNCC 7 cut(s) 71, 117, 118, 178, 928, 929, 1022
PstI CTGCAG 1 cut(s) 253
PsyI GACNNNGTC 2 cut(s) 87, 1020
RsaI GTAC 3 cut(s) 547, 803, 897
RsaNI GTAC 3 cut(s) 546, 802, 896
RseI CAYNNNNRTG 2 cut(s) 764, 776
SaqAI TTAA 5 cut(s) 32, 110, 407, 1065, 1148
SatI GCNGC 3 cut(s) 527, 621, 645
Sau3AI GATC 1 cut(s) 162
Sau96I GGNCC 7 cut(s) 71, 117, 118, 178, 928, 929, 1022
SchI GAGTC 3 cut(s) 185, 355, 476
SduI GDGCHC 5 cut(s) 44, 121, 714, 771, 932
SfaNI GCATC 2 cut(s) 651, 656
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 3 cut(s) 71, 178, 1022
SmiMI CAYNNNNRTG 2 cut(s) 764, 776
SmlI CTYRAG 3 cut(s) 349, 977, 1064
SmoI CTYRAG 3 cut(s) 349, 977, 1064
Sse9I AATT 7 cut(s) 111, 146, 255, 297, 322, 462, 598
SseBI AGGCCT 1 cut(s) 733
SsiI CCGC 1 cut(s) 723
SspI AATATT 1 cut(s) 345
SspMI CTAG 2 cut(s) 668, 821
StuI AGGCCT 1 cut(s) 733
TaiI ACGT 2 cut(s) 59, 580
TaqI TCGA 2 cut(s) 101, 714
TasI AATT 7 cut(s) 111, 146, 255, 297, 322, 462, 598
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 7 cut(s) 64, 271, 535, 781, 1029, 1072, 1084
Tru1I TTAA 5 cut(s) 32, 110, 407, 1065, 1148
Tru9I TTAA 5 cut(s) 32, 110, 407, 1065, 1148
TscAI CASTG 3 cut(s) 210, 505, 640
TseI GCWGC 3 cut(s) 526, 620, 644
TspDTI ATGAA 4 cut(s) 498, 577, 604, 776
TspGWI ACGGA 2 cut(s) 1166, 1166
TspRI CASTG 3 cut(s) 210, 505, 640
Tth111I GACNNNGTC 2 cut(s) 87, 1020
Vha464I CTTAAG 1 cut(s) 1064
VpaK11BI GGWCC 3 cut(s) 71, 178, 1022
VspI ATTAAT 2 cut(s) 32, 110
XceI RCATGY 3 cut(s) 743, 1001, 1009
XspI CTAG 2 cut(s) 668, 821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.