RchiOBHm_Chr1g0345051

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001937
Physical Location & Seq
Forward (+)
37453130 .. 37457210
4081 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57144

Sequence Viewer

Length: 1380 bp
ATGAAGATTTTGAAGCAGCAGCAAATGGAAGGTGAGAAAATGGAAGCAGAGCTATTAGTGGTAGCAGCAAAGATCATATTGTCGGTATTGGTGGGTGGGTTTCTGCTGTTGTTCTTGTATCTATATGAACTACTGATATTGAGGCCTAAAGGGCTAAGATCAAAGCTCCAAAAGCAGGGAATTAGAGGGCCTCCTCCGTCATTTCTGTTGGGGAATATCCTTGCATTAAAGAGACTTCAAAGCAAGGTCCAGTCATCTGCATCAAAGACTACAGATCCTCAAAAGCCCTATCATGATGATGATCGTGTGGCTTCTATTTGTCATCAATGGCCTTCAAAAATCTTCCCCCACATAGAGGAATGGAGAAATGAATATGGGCCAACATTTATGTATGCACTTGTCCACATGCAACAATTATGTATAACAGATGTAGAGCTATTGAAGGAACTTGGGCTTTGCAAGTCATTAATCTTGGGCACTCCTTCCATAACGTCCAAGATTCTCGGACCTCTTCTGGGACAGGGTCTTTTATCATCGAATGGATTAATTTGGGCCCATCAAAGGAAAACTATCGCTCCTGAATTATACCTTGCTAAGATCAAGGGCATGATGGACCTAATAGTGGACTCTACAACCTCAGTGCTAAGAACTTGGGAAAGTGAAATCCAAACGCAGGGAGGAACTGCAGAAATTAGAATTGATGAGAATCTAAGAAACATATCTGGAGATATAATTGCAAAAGCTTGTTTTCAGAGCAATTATAGTGAAGGAGAAGAAATATTCTCAAGACTTAGGACTCTACAAAAGGTTTTGTCAAAGGGTATGATGGGGGTAGAAGGCTTAATCAGATGGTTGCCAACAATCGAAAATAGAGAAATATGGAGATTAGAGAAGGAAATTGAGTCAATGATATTGAAGGTGGTAAAACAGCGCAGTGAAGCTTCATATGAGAAAAATTTGCTGCAAATGATTCTTCAGGGTGCTGAAACTTCGGGTGAAAATGGCCTATCACATAAGAAGTTCATTGTTGATAATTGCAAAACCATATTCATTGCTGCCCATGAGACCACTGCCGTTGCAGCATCATGGAGCTTGATGCTACTAGCTTTACATCCAGATTGGCAAGCTCGTGTTCGTGCTGAGGTGCTCGAAATCTGCGGGGATAGGCCTCTACATGCAGATATGCTTCCAAGCATGAAAGTGCTCACAATGGTGATTCAGGAAGTATTGCGTCTATACTCTCCTGTATTTTTTTTAGCAAGAGAGGTCCTTGAAACTATCACATTGAAAAACATTGTGATTCCAAAGGGTGTGGTCCTTCAGATACCAGTACCCTTTCTACACCAGAACCCTGATATATGGGGCCCTGACGCATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

459

Amino Acids

51.83

Weight (kDa)

8.37

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 115 - 457 6.7e-51 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1158
AclWI GGATC 1 cut(s) 269
AcsI RAATTY 1 cut(s) 955
AcuI CTGAAG 2 cut(s) 959, 1304
AfaI GTAC 1 cut(s) 1332
AfiI CCNNNNNNNGG 6 cut(s) 175, 355, 515, 561, 622, 673
AgsI TTSAA 7 cut(s) 13, 239, 336, 442, 916, 1274, 1288
AleI CACNNNNGTG 1 cut(s) 1211
AluBI AGCT 8 cut(s) 52, 166, 436, 743, 941, 1092, 1106, 1127
AluI AGCT 8 cut(s) 52, 166, 436, 743, 941, 1092, 1106, 1127
Alw21I GWGCWC 2 cut(s) 1149, 1206
Alw26I GTCTC 2 cut(s) 226, 1058
AlwI GGATC 1 cut(s) 269
AoxI GGCC 8 cut(s) 143, 188, 329, 377, 552, 1003, 1166, 1363
ApaI GGGCCC 2 cut(s) 556, 1367
ApeKI GCWGC 6 cut(s) 16, 19, 65, 961, 1055, 1079
ApoI RAATTY 1 cut(s) 955
AseI ATTAAT 2 cut(s) 467, 545
AspLEI GCGC 1 cut(s) 933
AsuHPI GGTGA 3 cut(s) 44, 1007, 1225
AvaII GGWCC 5 cut(s) 247, 506, 613, 1267, 1315
BaeGI GKGCMC 3 cut(s) 479, 556, 1367
BanII GRGCYC 2 cut(s) 556, 1367
BauI CACGAG 1 cut(s) 1128
Bbv12I GWGCWC 2 cut(s) 1149, 1206
BbvCI CCTCAGC 1 cut(s) 1140
BbvI GCAGC 6 cut(s) 28, 31, 77, 948, 1042, 1091
BccI CCATC 4 cut(s) 564, 604, 820, 843
BceAI ACGGC 1 cut(s) 1058
BcoDI GTCTC 2 cut(s) 226, 1058
BfaI CTAG 1 cut(s) 1103
BfmI CTRYAG 2 cut(s) 270, 684
BglI GCCNNNNNGGC 1 cut(s) 151
BisI GCNGC 6 cut(s) 17, 20, 66, 962, 1056, 1080
BlsI GCNGC 6 cut(s) 18, 21, 67, 963, 1057, 1081
Bme18I GGWCC 5 cut(s) 247, 506, 613, 1267, 1315
BmiI GGNNCC 3 cut(s) 554, 1364, 1365
BmsI GCATC 3 cut(s) 269, 1086, 1091
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 1 cut(s) 1140
BpuEI CTTGAG 1 cut(s) 769
BsaBI GATNNNNATC 2 cut(s) 300, 705
BsaI GGTCTC 1 cut(s) 1058
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 6 cut(s) 175, 355, 515, 561, 622, 673
Bse1I ACTGG 2 cut(s) 250, 1328
Bse3DI GCAATG 1 cut(s) 1050
Bse8I GATNNNNATC 2 cut(s) 300, 705
BseGI GGATG 1 cut(s) 1111
BseJI GATNNNNATC 2 cut(s) 300, 705
BseLI CCNNNNNNNGG 6 cut(s) 175, 355, 515, 561, 622, 673
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 2 cut(s) 651, 1131
BseNI ACTGG 2 cut(s) 250, 1328
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 3 cut(s) 479, 556, 1367
BseXI GCAGC 6 cut(s) 28, 31, 77, 948, 1042, 1091
BshFI GGCC 8 cut(s) 145, 190, 331, 379, 554, 1005, 1168, 1365
BsiHKAI GWGCWC 2 cut(s) 1149, 1206
BslFI GGGAC 1 cut(s) 531
BslI CCNNNNNNNGG 6 cut(s) 175, 355, 515, 561, 622, 673
BsmAI GTCTC 2 cut(s) 226, 1058
BsmFI GGGAC 1 cut(s) 531
BsnI GGCC 8 cut(s) 145, 190, 331, 379, 554, 1005, 1168, 1365
Bso31I GGTCTC 1 cut(s) 1058
Bsp120I GGGCCC 2 cut(s) 552, 1363
Bsp1286I GDGCHC 5 cut(s) 479, 556, 1149, 1206, 1367
Bsp143I GATC 5 cut(s) 72, 158, 274, 301, 597
BspACI CCGC 1 cut(s) 1158
BspANI GGCC 8 cut(s) 145, 190, 331, 379, 554, 1005, 1168, 1365
BspCNI CTCAG 2 cut(s) 650, 1132
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 3 cut(s) 554, 1364, 1365
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 1 cut(s) 269
BspTNI GGTCTC 1 cut(s) 1058
BsrDI GCAATG 1 cut(s) 1050
BsrI ACTGG 2 cut(s) 250, 1328
BssMI GATC 5 cut(s) 72, 158, 274, 301, 597
BssSI CACGAG 1 cut(s) 1128
Bst2BI CACGAG 1 cut(s) 1128
Bst6I CTCTTC 1 cut(s) 516
BstC8I GCNNGC 1 cut(s) 1125
BstDEI CTNAG 7 cut(s) 155, 594, 637, 644, 710, 791, 1140
BstF5I GGATG 1 cut(s) 1111
BstHHI GCGC 1 cut(s) 933
BstKTI GATC 5 cut(s) 75, 161, 277, 304, 600
BstMAI GTCTC 2 cut(s) 226, 1058
BstMBI GATC 5 cut(s) 72, 158, 274, 301, 597
BstMWI GCNNNNNNNGC 3 cut(s) 151, 172, 1079
BstNSI RCATGY 2 cut(s) 409, 1178
BstSFI CTRYAG 2 cut(s) 270, 684
BstSLI GKGCMC 3 cut(s) 479, 556, 1367
BstV1I GCAGC 6 cut(s) 28, 31, 77, 948, 1042, 1091
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 8 cut(s) 145, 190, 331, 379, 554, 1005, 1168, 1365
BtsCI GGATG 1 cut(s) 1111
BtsI GCAGTG 2 cut(s) 940, 1068
BtsIMutI CAGTG 3 cut(s) 645, 940, 1068
Cac8I GCNNGC 1 cut(s) 1125
CciI TCATGA 1 cut(s) 292
CfoI GCGC 1 cut(s) 933
CseI GACGC 1 cut(s) 1220
Csp6I GTAC 1 cut(s) 1331
CspCI CAANNNNNGTGG 4 cut(s) 1057, 1092, 1293, 1328
CviAII CATG 7 cut(s) 293, 406, 607, 1061, 1086, 1175, 1195
CviQI GTAC 1 cut(s) 1331
DdeI CTNAG 7 cut(s) 155, 594, 637, 644, 710, 791, 1140
DpnI GATC 5 cut(s) 74, 160, 276, 303, 599
DpnII GATC 5 cut(s) 72, 158, 274, 301, 597
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco147I AGGCCT 2 cut(s) 145, 1168
Eco24I GRGCYC 2 cut(s) 556, 1367
Eco31I GGTCTC 1 cut(s) 1058
Eco47I GGWCC 5 cut(s) 247, 506, 613, 1267, 1315
Eco57I CTGAAG 2 cut(s) 959, 1304
EcoO109I RGGNCCY 4 cut(s) 188, 1267, 1363, 1364
EcoT38I GRGCYC 2 cut(s) 556, 1367
FaeI CATG 7 cut(s) 296, 409, 610, 1064, 1089, 1178, 1198
FaqI GGGAC 1 cut(s) 531
FatI CATG 7 cut(s) 292, 405, 606, 1060, 1085, 1174, 1194
FauI CCCGC 1 cut(s) 1151
FauNDI CATATG 1 cut(s) 946
Fnu4HI GCNGC 6 cut(s) 17, 20, 66, 962, 1056, 1080
FokI GGATG 1 cut(s) 1098
FriOI GRGCYC 2 cut(s) 556, 1367
Fsp4HI GCNGC 6 cut(s) 17, 20, 66, 962, 1056, 1080
FspBI CTAG 1 cut(s) 1103
GlaI GCGC 1 cut(s) 932
GluI GCNGC 6 cut(s) 17, 20, 66, 962, 1056, 1080
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 8 cut(s) 145, 190, 331, 379, 554, 1005, 1168, 1365
HgaI GACGC 1 cut(s) 1220
HhaI GCGC 1 cut(s) 933
Hin1II CATG 7 cut(s) 296, 409, 610, 1064, 1089, 1178, 1198
Hin6I GCGC 1 cut(s) 931
HinP1I GCGC 1 cut(s) 931
HindIII AAGCTT 2 cut(s) 741, 939
HinfI GANTC 8 cut(s) 499, 626, 706, 796, 902, 970, 1216, 1300
HphI GGTGA 3 cut(s) 44, 1007, 1225
Hpy166II GTNNAC 2 cut(s) 403, 625
Hpy188I TCNGA 4 cut(s) 506, 753, 848, 1323
Hpy188III TCNNGA 6 cut(s) 293, 578, 723, 786, 1115, 1220
Hpy8I GTNNAC 2 cut(s) 403, 625
HpyAV CCTTC 9 cut(s) 23, 342, 436, 492, 761, 830, 886, 910, 1328
HpyCH4IV ACGT 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 172, 1079
HpyF3I CTNAG 7 cut(s) 155, 594, 637, 644, 710, 791, 1140
HpySE526I ACGT 1 cut(s) 491
Hsp92II CATG 7 cut(s) 296, 409, 610, 1064, 1089, 1178, 1198
HspAI GCGC 1 cut(s) 931
Kzo9I GATC 5 cut(s) 72, 158, 274, 301, 597
LmnI GCTCC 3 cut(s) 171, 580, 1089
Lsp1109I GCAGC 6 cut(s) 28, 31, 77, 948, 1042, 1091
LweI GCATC 3 cut(s) 269, 1086, 1091
MaeI CTAG 1 cut(s) 1103
MaeII ACGT 1 cut(s) 491
MalI GATC 5 cut(s) 74, 160, 276, 303, 599
MboI GATC 5 cut(s) 72, 158, 274, 301, 597
MboII GAAGA 5 cut(s) 16, 334, 503, 785, 965
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 5 cut(s) 479, 556, 1149, 1206, 1367
MlyI GAGTC 3 cut(s) 620, 790, 911
MseI TTAA 4 cut(s) 227, 467, 545, 842
MslI CAYNNNNRTG 3 cut(s) 297, 1199, 1211
MwoI GCNNNNNNNGC 3 cut(s) 151, 172, 1079
NdeI CATATG 1 cut(s) 946
NdeII GATC 5 cut(s) 72, 158, 274, 301, 597
NlaIII CATG 7 cut(s) 296, 409, 610, 1064, 1089, 1178, 1198
NlaIV GGNNCC 3 cut(s) 554, 1364, 1365
NspI RCATGY 2 cut(s) 409, 1178
OliI CACNNNNGTG 1 cut(s) 1211
PagI TCATGA 1 cut(s) 292
PceI AGGCCT 2 cut(s) 145, 1168
PfeI GAWTC 5 cut(s) 499, 706, 970, 1216, 1300
PflFI GACNNNGTC 1 cut(s) 522
PkrI GCNGC 6 cut(s) 18, 21, 67, 963, 1057, 1081
PleI GAGTC 3 cut(s) 620, 790, 910
PpsI GAGTC 3 cut(s) 620, 790, 910
PpuMI RGGWCCY 1 cut(s) 1267
PshBI ATTAAT 2 cut(s) 467, 545
Psp5II RGGWCCY 1 cut(s) 1267
PspN4I GGNNCC 3 cut(s) 554, 1364, 1365
PspOMI GGGCCC 2 cut(s) 552, 1363
PspPPI RGGWCCY 1 cut(s) 1267
PstI CTGCAG 1 cut(s) 688
PsuI RGATCY 1 cut(s) 274
PsyI GACNNNGTC 1 cut(s) 522
RsaI GTAC 1 cut(s) 1332
RsaNI GTAC 1 cut(s) 1331
RseI CAYNNNNRTG 3 cut(s) 297, 1199, 1211
SaqAI TTAA 4 cut(s) 227, 467, 545, 842
SatI GCNGC 6 cut(s) 17, 20, 66, 962, 1056, 1080
Sau3AI GATC 5 cut(s) 72, 158, 274, 301, 597
SchI GAGTC 3 cut(s) 620, 790, 911
SduI GDGCHC 5 cut(s) 479, 556, 1149, 1206, 1367
SfaNI GCATC 3 cut(s) 269, 1086, 1091
SfcI CTRYAG 2 cut(s) 270, 684
SinI GGWCC 5 cut(s) 247, 506, 613, 1267, 1315
SmiMI CAYNNNNRTG 3 cut(s) 297, 1199, 1211
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SseBI AGGCCT 2 cut(s) 145, 1168
SsiI CCGC 1 cut(s) 1158
SspI AATATT 1 cut(s) 780
SspMI CTAG 1 cut(s) 1103
StuI AGGCCT 2 cut(s) 145, 1168
TaiI ACGT 1 cut(s) 494
TaqI TCGA 3 cut(s) 536, 864, 1149
TfiI GAWTC 5 cut(s) 499, 706, 970, 1216, 1300
Tru1I TTAA 4 cut(s) 227, 467, 545, 842
Tru9I TTAA 4 cut(s) 227, 467, 545, 842
TscAI CASTG 3 cut(s) 645, 940, 1075
TseI GCWGC 6 cut(s) 16, 19, 65, 961, 1055, 1079
TspDTI ATGAA 7 cut(s) 17, 141, 384, 933, 1012, 1039, 1211
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 3 cut(s) 645, 940, 1075
Tth111I GACNNNGTC 1 cut(s) 522
VpaK11BI GGWCC 5 cut(s) 247, 506, 613, 1267, 1315
VspI ATTAAT 2 cut(s) 467, 545
XapI RAATTY 1 cut(s) 955
XceI RCATGY 2 cut(s) 409, 1178
XspI CTAG 1 cut(s) 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.