Rh1AG193400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001937
Physical Location & Seq
Forward (+)
36918822 .. 36924777
5956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG193400.1

Sequence Viewer

Length: 1482 bp
ATGGAAGCAGAGTTAGCAGTAGCTGCAAAGATGGTAGTACTGTCAGTATTGGTGTGTGGGCTTTTGTTCTTGGTTTCATATTTGTGTGAACTTCTGCTGTTGAGGCCTAAATCCATAAGATCAAAGCTGGAAAAGCAAGGAATTCGAGGGCCGCGTCCTACCTTTCTATTGGGAAACCTCCCTGAACAGAAGAGGCTCCAAAGCAAGCAGCAGGGTCAGTCAATAGCAACAAGGACTGATGATGATGATCATGCAGCTTCTTTTTCTCATCAATGGTATTTGAAAATCTTCCCCCACATAGAGCAATGGAGAAATGAATACGGTCCAATATTTTTGTGTTCATCTGCACACATACAACAATTAGTAATAACAGATATGGAAGTGGTGAAGGAAATTAGCTTGTGTAAGTCTCTAGTCTTGGGAAATCCTTCTGTAACATCCAAGGTTCTTGGGTCACTATTGGGAGAGGGCATTTTAGCGTCGAGCGGATCAATGTGGGAGCATCAAAGGAAAATTATCGCTCCTGAACTATACCTTGATAAGGTGAAGGGCATGATGAACCTAGCTGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGCTGCAAGAAGTTTTGGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACAGAAAATATAAAGATATGGATGTTGGAAAAGGAAACTCAATCACTGATATTGAAGGTGGTAAAACAACGTAGTGAAGGTTCATATGAGAAAGATTTGCTGCAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGATTTAAATGGCCTATCATACAATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCACTGCCCATGTAGCATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTACTTGAAATCTGTGGTGATAAGCCTCTAGATGCTGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGTACTCTTTGTAGCAAGAGAGGTATTTGAAACTATCAAATTTAAAAACATAACAATTCCAAAGGGGACGCTACTTCAAATACCAATCCCCTTCTTACACCAGAACCCTGATCTATGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTACCAATGGAATTGTCAAGGCTTGCAAGTCTCCACAGGCCTACATGCCATTCGGAGCAGGTTCTCGTATATGTGTTGGTCAACACTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATAATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGCATACCAGCATTCTCCTGCGTATAACATTATTCTGGAGCCTGAATATGGTATTATTCTACAAATGCGAAGAGCATCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

493

Amino Acids

55.55

Weight (kDa)

8.9

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 181 - 475 3.6e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1292
AccB1I GGYRCC 1 cut(s) 824
AccBSI CCGCTC 1 cut(s) 486
AccII CGCG 1 cut(s) 154
AciI CCGC 3 cut(s) 152, 486, 1088
AclWI GGATC 2 cut(s) 496, 1219
AcoI YGGCCR 1 cut(s) 904
AcsI RAATTY 2 cut(s) 141, 1130
AfaI GTAC 3 cut(s) 39, 993, 1095
AfiI CCNNNNNNNGG 2 cut(s) 541, 1445
AflII CTTAAG 1 cut(s) 1346
AgsI TTSAA 7 cut(s) 283, 760, 821, 998, 1121, 1169, 1231
AleI CACNNNNGTG 1 cut(s) 1058
Alw21I GWGCWC 2 cut(s) 982, 1053
Alw26I GTCTC 3 cut(s) 414, 905, 1278
AlwI GGATC 2 cut(s) 496, 1219
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 981
AoxI GGCC 5 cut(s) 104, 149, 850, 904, 1281
ApeKI GCWGC 6 cut(s) 23, 208, 254, 566, 643, 805
ApoI RAATTY 2 cut(s) 141, 1130
Asp700I GAANNNNTTC 2 cut(s) 287, 427
AspS9I GGNCC 2 cut(s) 149, 323
AsuHPI GGTGA 5 cut(s) 397, 556, 851, 1019, 1072
AvaI CYCGRG 1 cut(s) 981
AvaII GGWCC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 906
BanI GGYRCC 1 cut(s) 824
BauI CACGAG 1 cut(s) 975
Bbv12I GWGCWC 2 cut(s) 982, 1053
BbvCI CCTCAGC 1 cut(s) 987
BbvI GCAGC 6 cut(s) 10, 220, 266, 553, 630, 792
BccI CCATC 4 cut(s) 25, 664, 687, 966
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 3 cut(s) 414, 905, 1278
BfaI CTAG 4 cut(s) 413, 563, 950, 1019
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 1 cut(s) 1346
BfuAI ACCTGC 1 cut(s) 1292
BisI GCNGC 7 cut(s) 24, 152, 209, 255, 567, 644, 806
BlsI GCNGC 7 cut(s) 25, 153, 210, 256, 568, 645, 807
BmcAI AGTACT 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 323
BmeT110I CYCGRG 1 cut(s) 981
BmgT120I GGNCC 2 cut(s) 149, 323
BmiI GGNNCC 3 cut(s) 197, 826, 1437
BmsI GCATC 4 cut(s) 511, 938, 1012, 1207
BpmI CTGGAG 1 cut(s) 1454
Bpu10I CCTNAGC 1 cut(s) 987
BsaBI GATNNNNATC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 1 cut(s) 441
BsaXI ACNNNNNCTCC 2 cut(s) 939, 969
Bsc4I CCNNNNNNNGG 2 cut(s) 541, 1445
Bse3DI GCAATG 1 cut(s) 311
Bse8I GATNNNNATC 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 441
BseGI GGATG 3 cut(s) 437, 732, 958
BseJI GATNNNNATC 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 541, 1445
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 1 cut(s) 978
BseXI GCAGC 6 cut(s) 10, 220, 266, 553, 630, 792
BsgI GTGCAG 1 cut(s) 330
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 106, 151, 852, 906, 1283
BshNI GGYRCC 1 cut(s) 824
BsiHKAI GWGCWC 2 cut(s) 982, 1053
BsiHKCI CYCGRG 1 cut(s) 981
BslFI GGGAC 1 cut(s) 1171
BslI CCNNNNNNNGG 2 cut(s) 541, 1445
BsmAI GTCTC 3 cut(s) 414, 905, 1278
BsmFI GGGAC 1 cut(s) 1171
BsmI GAATGC 1 cut(s) 1408
BsnI GGCC 5 cut(s) 106, 151, 852, 906, 1283
Bso31I GGTCTC 1 cut(s) 905
BsoBI CYCGRG 1 cut(s) 981
Bsp1286I GDGCHC 2 cut(s) 982, 1053
Bsp143I GATC 5 cut(s) 119, 247, 488, 1201, 1211
BspACI CCGC 3 cut(s) 152, 486, 1088
BspANI GGCC 5 cut(s) 106, 151, 852, 906, 1283
BspCNI CTCAG 1 cut(s) 979
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 197, 826, 1437
BspMAI CTGCAG 1 cut(s) 571
BspMI ACCTGC 1 cut(s) 1292
BspPI GGATC 2 cut(s) 496, 1219
BspQI GCTCTTC 1 cut(s) 1462
BspT107I GGYRCC 1 cut(s) 824
BspTI CTTAAG 1 cut(s) 1346
BspTNI GGTCTC 1 cut(s) 905
BsrBI CCGCTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 311
BssECI CCNNGG 1 cut(s) 441
BssMI GATC 5 cut(s) 119, 247, 488, 1201, 1211
BssSI CACGAG 1 cut(s) 975
BssT1I CCWWGG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 975
Bst4CI ACNGT 2 cut(s) 42, 323
Bst6I CTCTTC 2 cut(s) 185, 1462
BstAFI CTTAAG 1 cut(s) 1346
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 5 cut(s) 206, 904, 941, 972, 1267
BstDEI CTNAG 1 cut(s) 987
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 3 cut(s) 437, 732, 958
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 5 cut(s) 122, 250, 491, 1204, 1214
BstMAI GTCTC 3 cut(s) 414, 905, 1278
BstMBI GATC 5 cut(s) 119, 247, 488, 1201, 1211
BstMWI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 811, 926
BstNSI RCATGY 1 cut(s) 1291
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 6 cut(s) 10, 220, 266, 553, 630, 792
BstX2I RGATCY 1 cut(s) 1211
BstYI RGATCY 1 cut(s) 1211
BsuRI GGCC 5 cut(s) 106, 151, 852, 906, 1283
BtsCI GGATG 3 cut(s) 437, 732, 958
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 2 cut(s) 749, 915
BveI ACCTGC 1 cut(s) 1292
Cac8I GCNNGC 5 cut(s) 206, 904, 941, 972, 1267
CaiI CAGNNNCTG 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 149, 323
CseI GACGC 4 cut(s) 143, 468, 1067, 1168
Csp6I GTAC 3 cut(s) 38, 992, 1094
CviAII CATG 6 cut(s) 251, 553, 908, 923, 933, 1288
CviQI GTAC 3 cut(s) 38, 992, 1094
DdeI CTNAG 1 cut(s) 987
DpnI GATC 5 cut(s) 121, 249, 490, 1203, 1213
DpnII GATC 5 cut(s) 119, 247, 488, 1201, 1211
DraI TTTAAA 2 cut(s) 846, 1135
EaeI YGGCCR 1 cut(s) 904
Eam1104I CTCTTC 2 cut(s) 185, 1462
EarI CTCTTC 2 cut(s) 185, 1462
Eco130I CCWWGG 1 cut(s) 441
Eco147I AGGCCT 2 cut(s) 106, 1283
Eco31I GGTCTC 1 cut(s) 905
Eco47I GGWCC 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 981
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 441
FaeI CATG 6 cut(s) 254, 556, 911, 926, 936, 1291
FalI AAGNNNNNCTT 2 cut(s) 1367, 1399
FaqI GGGAC 1 cut(s) 1171
FatI CATG 6 cut(s) 250, 552, 907, 922, 932, 1287
FauI CCCGC 1 cut(s) 1095
FauNDI CATATG 1 cut(s) 790
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 7 cut(s) 24, 152, 209, 255, 567, 644, 806
FokI GGATG 3 cut(s) 424, 739, 945
Fsp4HI GCNGC 7 cut(s) 24, 152, 209, 255, 567, 644, 806
FspBI CTAG 4 cut(s) 413, 563, 950, 1019
GluI GCNGC 7 cut(s) 24, 152, 209, 255, 567, 644, 806
GsuI CTGGAG 1 cut(s) 1454
HaeIII GGCC 5 cut(s) 106, 151, 852, 906, 1283
HgaI GACGC 4 cut(s) 143, 468, 1067, 1168
Hin1II CATG 6 cut(s) 254, 556, 911, 926, 936, 1291
HincII GTYRAC 1 cut(s) 1325
HindII GTYRAC 1 cut(s) 1325
HindIII AAGCTT 1 cut(s) 585
HinfI GANTC 1 cut(s) 1063
HphI GGTGA 5 cut(s) 397, 556, 851, 1019, 1072
Hpy166II GTNNAC 2 cut(s) 89, 1325
Hpy188I TCNGA 3 cut(s) 702, 1216, 1298
Hpy188III TCNNGA 6 cut(s) 524, 818, 962, 1019, 1067, 1433
Hpy8I GTNNAC 2 cut(s) 89, 1325
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 7 cut(s) 382, 438, 541, 754, 776, 815, 1192
HpyCH4III ACNGT 2 cut(s) 42, 323
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 8 cut(s) 26, 254, 347, 569, 646, 808, 885, 1269
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 811, 926
HpyF3I CTNAG 1 cut(s) 987
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 6 cut(s) 254, 556, 911, 926, 936, 1291
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 5 cut(s) 119, 247, 488, 1201, 1211
LguI GCTCTTC 1 cut(s) 1462
LmnI GCTCC 6 cut(s) 201, 499, 526, 936, 1298, 1435
Lsp1109I GCAGC 6 cut(s) 10, 220, 266, 553, 630, 792
LweI GCATC 4 cut(s) 511, 938, 1012, 1207
MaeI CTAG 4 cut(s) 413, 563, 950, 1019
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 3 cut(s) 433, 453, 945
MalI GATC 5 cut(s) 121, 249, 490, 1203, 1213
MbiI CCGCTC 1 cut(s) 486
MboI GATC 5 cut(s) 119, 247, 488, 1201, 1211
MboII GAAGA 3 cut(s) 202, 280, 1479
MflI RGATCY 1 cut(s) 1211
MhlI GDGCHC 2 cut(s) 982, 1053
MlsI TGGCCA 1 cut(s) 906
MluNI TGGCCA 1 cut(s) 906
MmeI TCCRAC 2 cut(s) 711, 725
Mox20I TGGCCA 1 cut(s) 906
MroXI GAANNNNTTC 2 cut(s) 287, 427
MscI TGGCCA 1 cut(s) 906
MseI TTAA 3 cut(s) 845, 1134, 1347
MslI CAYNNNNRTG 2 cut(s) 82, 1058
Msp20I TGGCCA 1 cut(s) 906
MspCI CTTAAG 1 cut(s) 1346
Mva1269I GAATGC 1 cut(s) 1408
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 811, 926
NdeI CATATG 1 cut(s) 790
NdeII GATC 5 cut(s) 119, 247, 488, 1201, 1211
NlaIII CATG 6 cut(s) 254, 556, 911, 926, 936, 1291
NlaIV GGNNCC 3 cut(s) 197, 826, 1437
NmuCI GTSAC 1 cut(s) 453
NspI RCATGY 1 cut(s) 1291
OliI CACNNNNGTG 1 cut(s) 1058
PaeR7I CTCGAG 1 cut(s) 981
PceI AGGCCT 2 cut(s) 106, 1283
PciSI GCTCTTC 1 cut(s) 1462
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PctI GAATGC 1 cut(s) 1408
PdmI GAANNNNTTC 2 cut(s) 287, 427
PfeI GAWTC 1 cut(s) 1063
PkrI GCNGC 7 cut(s) 25, 153, 210, 256, 568, 645, 807
PspN4I GGNNCC 3 cut(s) 197, 826, 1437
PspPI GGNCC 2 cut(s) 149, 323
PspXI VCTCGAGB 1 cut(s) 981
PsrI GAACNNNNNNTAC 2 cut(s) 769, 801
PstI CTGCAG 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 1211
RsaI GTAC 3 cut(s) 39, 993, 1095
RsaNI GTAC 3 cut(s) 38, 992, 1094
RseI CAYNNNNRTG 2 cut(s) 82, 1058
SapI GCTCTTC 1 cut(s) 1462
SaqAI TTAA 3 cut(s) 845, 1134, 1347
SatI GCNGC 7 cut(s) 24, 152, 209, 255, 567, 644, 806
Sau3AI GATC 5 cut(s) 119, 247, 488, 1201, 1211
Sau96I GGNCC 2 cut(s) 149, 323
ScaI AGTACT 1 cut(s) 39
SduI GDGCHC 2 cut(s) 982, 1053
SfaNI GCATC 4 cut(s) 511, 938, 1012, 1207
SfcI CTRYAG 1 cut(s) 567
Sfr274I CTCGAG 1 cut(s) 981
SinI GGWCC 1 cut(s) 323
SlaI CTCGAG 1 cut(s) 981
SmiI ATTTAAAT 1 cut(s) 846
SmiMI CAYNNNNRTG 2 cut(s) 82, 1058
SmlI CTYRAG 2 cut(s) 981, 1346
SmoI CTYRAG 2 cut(s) 981, 1346
SseBI AGGCCT 2 cut(s) 106, 1283
SsiI CCGC 3 cut(s) 152, 486, 1088
SspI AATATT 2 cut(s) 330, 624
SspMI CTAG 4 cut(s) 413, 563, 950, 1019
StuI AGGCCT 2 cut(s) 106, 1283
StyI CCWWGG 1 cut(s) 441
SwaI ATTTAAAT 1 cut(s) 846
TaaI ACNGT 2 cut(s) 42, 323
TaiI ACGT 1 cut(s) 778
TaqI TCGA 4 cut(s) 145, 482, 982, 1036
TatI WGTACW 2 cut(s) 37, 1093
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 3 cut(s) 845, 1134, 1347
Tru9I TTAA 3 cut(s) 845, 1134, 1347
TscAI CASTG 2 cut(s) 756, 922
TseFI GTSAC 1 cut(s) 453
TseI GCWGC 6 cut(s) 23, 208, 254, 566, 643, 805
Tsp45I GTSAC 1 cut(s) 453
TspDTI ATGAA 7 cut(s) 66, 330, 330, 572, 584, 777, 1058
TspRI CASTG 2 cut(s) 756, 922
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 1 cut(s) 323
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 2 cut(s) 141, 1130
XbaI TCTAGA 1 cut(s) 1018
XceI RCATGY 1 cut(s) 1291
XhoI CTCGAG 1 cut(s) 981
XmnI GAANNNNTTC 2 cut(s) 287, 427
XspI CTAG 4 cut(s) 413, 563, 950, 1019
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.