RchiOBHm_Chr1g0344991

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001937
Physical Location & Seq
Forward (+)
37366975 .. 37371205
4231 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57138

Sequence Viewer

Length: 1596 bp
ATGGAAGCAGAGTTAGCAGTAGCTGCAAAGATGGTAGTACTGTCAGTATTGGTGTGTGGGCTTTTGTTCTTGGTTTCATATTTGTGTGAACTTCTGCTGTTGAGGCCTAAATCCATAAGATCAAAGCTGGAAAAGCAAGGAATTCGAGGGCCGCGTCCTACCTTTCTATTGGGAAACCTCCCTGAACAGAAGAGGCTCCAAAGCAAGCAGCAGGGTCAGTCAATAGCAACAAGGACTGATGATGATGATGATGATGATGATCATGCAGCTTATTTTTCTCATCAATGGTATTTGAAAATCTTCCCCCACATAGAGCAATGGAGAAATGAATACGGTCCAATATTTTTGTGTTCATCTGCACACATACAACAATTAGTAATAACAGATATGGAAGTGGTGAAGGAAATTAGCTTGTGTAAGTCTCTAGTCTTGGGAAATCCTTCTGTAACATCCAAGGTTCTTGGGTCACTATTGGGAGAGGGCATTTTAGCGTCGAACGGATCAATGTGGGAGCATCAAAGGAAAATTATCGCTCCTGAACTATACCTTGATAAGGTGAAGGGCATGATGAACCTGGTAGTCGATTCTACAACCACAATGCTAAGAACTTGGGAAAATGAAGTCCAAAGGCAGGGAGAACTTGCTGAAATTAGAGTTGATGATCATTTGAGAAACTTATCAGCAGATGTCATCTCAAAAGCTTGTTTTCATAGCAATTATCATCAAGGAAAAGAAATATTCTCAAAAATTAGGAAGCTGCAAGAAGTTTTGGGAAAGGGATTGATGGGGATAGAAGCCTTGACCAGATGGTTTCCGACAACAGAAAATATAAAGATATGGATGTTGGAAAAGGAAACTCAATCACTGATATTGAAGGTGGTAAAACAACGTAGTGAAGGTTCATATGAGAAAGATTTGCTGCAAATGCTTCTTGAAGGTGCCAAAAGTTCTGGTGATTTAAATGGCCTATCATACAATAAGTTTATTGTTGATAACTGCAAGACTATATTCTTTGCTGGCCATGAGACCACTGCCCTTGTAGCATCATGGAGCTTGCTGTTACTAGCTCTCCATCCAGATTGGCAAGCTCGTGCTCGAGCTGAGGTACTTGAAATCTGTGGTGATAAGCCTCTAGATGCTGATATGCTTCGACAAATGAAAGTGCTCACAATGGTGATTCAGGAGGTATTGCGTCTTTACCCGCCTGTACTCTTTGTAGCAAGAGAGGTATTTGAAACTATCAAATTTAAAAACATAACAATTCCAAAGGGGACGCTACTTCAAATACCAATCCCCTTCTTACACCAGAACCCTGATCTATGGGGATCTGATGCTCATAAGTTCAACCCAGAAAGATTTACCAATGGAATTGTCAAGGCTTCCAAGTCTCCACAGGCCTACATGCCATTCGGAGCAGGTTCTCGTATATGTGTTGGTCAACACTTGGCTATGATAGAACTTAAGGTGTTTCTTTCTATAATTCTCTCAAAGTTCTGCTTCTCCCTCTCATCAGCATACCAGCATTCTCCTGCGTATAACATTATTCTGGAGCCTGAATATGGTATTATTCTACAAATGCGAAGAGCATCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

531

Amino Acids

60.1

Weight (kDa)

7.98

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 101 - 513 2.8e-82 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1406
AccB1I GGYRCC 1 cut(s) 938
AccII CGCG 1 cut(s) 154
AciI CCGC 2 cut(s) 152, 1202
AclWI GGATC 2 cut(s) 508, 1333
AcoI YGGCCR 1 cut(s) 1018
AcsI RAATTY 2 cut(s) 141, 1244
AfaI GTAC 3 cut(s) 39, 1107, 1209
AfiI CCNNNNNNNGG 3 cut(s) 553, 631, 1559
AflII CTTAAG 1 cut(s) 1460
AgsI TTSAA 7 cut(s) 295, 874, 935, 1112, 1235, 1283, 1345
AjnI CCWGG 1 cut(s) 573
AleI CACNNNNGTG 1 cut(s) 1172
Alw21I GWGCWC 2 cut(s) 1096, 1167
Alw26I GTCTC 3 cut(s) 426, 1019, 1392
AlwI GGATC 2 cut(s) 508, 1333
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 1095
AoxI GGCC 5 cut(s) 104, 149, 964, 1018, 1395
ApeKI GCWGC 5 cut(s) 23, 208, 266, 757, 919
ApoI RAATTY 2 cut(s) 141, 1244
Asp700I GAANNNNTTC 2 cut(s) 299, 439
AspS9I GGNCC 2 cut(s) 149, 335
AsuHPI GGTGA 5 cut(s) 409, 568, 965, 1133, 1186
AvaI CYCGRG 1 cut(s) 1095
AvaII GGWCC 1 cut(s) 335
BalI TGGCCA 1 cut(s) 1020
BanI GGYRCC 1 cut(s) 938
BauI CACGAG 1 cut(s) 1089
Bbv12I GWGCWC 2 cut(s) 1096, 1167
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 5 cut(s) 10, 220, 278, 744, 906
BccI CCATC 4 cut(s) 25, 778, 801, 1080
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 1 cut(s) 575
BclI TGATCA 2 cut(s) 259, 661
BcoDI GTCTC 3 cut(s) 426, 1019, 1392
BfaI CTAG 3 cut(s) 425, 1064, 1133
BfrI CTTAAG 1 cut(s) 1460
BfuAI ACCTGC 1 cut(s) 1406
BisI GCNGC 6 cut(s) 24, 152, 209, 267, 758, 920
BlsI GCNGC 6 cut(s) 25, 153, 210, 268, 759, 921
BmcAI AGTACT 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 1095
BmgT120I GGNCC 2 cut(s) 149, 335
BmiI GGNNCC 3 cut(s) 197, 940, 1551
BmrFI CCNGG 1 cut(s) 575
BmsI GCATC 4 cut(s) 523, 1052, 1126, 1321
BpmI CTGGAG 1 cut(s) 1568
Bpu10I CCTNAGC 1 cut(s) 1101
BsaBI GATNNNNATC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 1019
BsaJI CCNNGG 1 cut(s) 453
BsaXI ACNNNNNCTCC 2 cut(s) 1053, 1083
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 631, 1559
Bse3DI GCAATG 1 cut(s) 323
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 453
BseGI GGATG 3 cut(s) 449, 846, 1072
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 3 cut(s) 553, 631, 1559
BseMI GCAATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 1092
BseXI GCAGC 5 cut(s) 10, 220, 278, 744, 906
BsgI GTGCAG 1 cut(s) 342
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 106, 151, 966, 1020, 1397
BshNI GGYRCC 1 cut(s) 938
BsiHKAI GWGCWC 2 cut(s) 1096, 1167
BsiHKCI CYCGRG 1 cut(s) 1095
BslFI GGGAC 1 cut(s) 1285
BslI CCNNNNNNNGG 3 cut(s) 553, 631, 1559
BsmAI GTCTC 3 cut(s) 426, 1019, 1392
BsmFI GGGAC 1 cut(s) 1285
BsmI GAATGC 1 cut(s) 1522
BsnI GGCC 5 cut(s) 106, 151, 966, 1020, 1397
Bso31I GGTCTC 1 cut(s) 1019
BsoBI CYCGRG 1 cut(s) 1095
Bsp1286I GDGCHC 2 cut(s) 1096, 1167
Bsp143I GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
BspACI CCGC 2 cut(s) 152, 1202
BspANI GGCC 5 cut(s) 106, 151, 966, 1020, 1397
BspCNI CTCAG 1 cut(s) 1093
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 197, 940, 1551
BspMI ACCTGC 1 cut(s) 1406
BspPI GGATC 2 cut(s) 508, 1333
BspQI GCTCTTC 1 cut(s) 1576
BspT107I GGYRCC 1 cut(s) 938
BspTI CTTAAG 1 cut(s) 1460
BspTNI GGTCTC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 323
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
BssSI CACGAG 1 cut(s) 1089
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 1089
Bst2UI CCWGG 1 cut(s) 575
Bst4CI ACNGT 2 cut(s) 42, 335
Bst6I CTCTTC 2 cut(s) 185, 1576
BstAFI CTTAAG 1 cut(s) 1460
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 4 cut(s) 206, 1018, 1055, 1086
BstDEI CTNAG 2 cut(s) 602, 1101
BstENI CCTNNNNNAGG 1 cut(s) 551
BstF5I GGATG 3 cut(s) 449, 846, 1072
BstFNI CGCG 1 cut(s) 154
BstKTI GATC 6 cut(s) 122, 262, 503, 664, 1318, 1328
BstMAI GTCTC 3 cut(s) 426, 1019, 1392
BstMBI GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
BstMWI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 925, 1040
BstNI CCWGG 1 cut(s) 575
BstNSI RCATGY 1 cut(s) 1405
BstSCI CCNGG 1 cut(s) 573
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 5 cut(s) 10, 220, 278, 744, 906
BstX2I RGATCY 1 cut(s) 1325
BstYI RGATCY 1 cut(s) 1325
BsuRI GGCC 5 cut(s) 106, 151, 966, 1020, 1397
BtsCI GGATG 3 cut(s) 449, 846, 1072
BtsI GCAGTG 1 cut(s) 1029
BtsIMutI CAGTG 2 cut(s) 863, 1029
BveI ACCTGC 1 cut(s) 1406
Cac8I GCNNGC 4 cut(s) 206, 1018, 1055, 1086
CaiI CAGNNNCTG 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 149, 335
CseI GACGC 4 cut(s) 143, 480, 1181, 1282
CsiI ACCWGGT 1 cut(s) 573
Csp6I GTAC 3 cut(s) 38, 1106, 1208
CspCI CAANNNNNGTGG 2 cut(s) 1018, 1053
CviAII CATG 5 cut(s) 263, 565, 1022, 1047, 1402
CviQI GTAC 3 cut(s) 38, 1106, 1208
DdeI CTNAG 2 cut(s) 602, 1101
DpnI GATC 6 cut(s) 121, 261, 502, 663, 1317, 1327
DpnII GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
DraI TTTAAA 2 cut(s) 960, 1249
EaeI YGGCCR 1 cut(s) 1018
Eam1104I CTCTTC 2 cut(s) 185, 1576
EarI CTCTTC 2 cut(s) 185, 1576
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 2 cut(s) 106, 1397
Eco31I GGTCTC 1 cut(s) 1019
Eco47I GGWCC 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 1095
EcoNI CCTNNNNNAGG 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 573
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 5 cut(s) 266, 568, 1025, 1050, 1405
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 1 cut(s) 1285
FatI CATG 5 cut(s) 262, 564, 1021, 1046, 1401
FauI CCCGC 1 cut(s) 1209
FauNDI CATATG 1 cut(s) 904
FbaI TGATCA 2 cut(s) 259, 661
Fnu4HI GCNGC 6 cut(s) 24, 152, 209, 267, 758, 920
FokI GGATG 3 cut(s) 436, 853, 1059
Fsp4HI GCNGC 6 cut(s) 24, 152, 209, 267, 758, 920
FspBI CTAG 3 cut(s) 425, 1064, 1133
GluI GCNGC 6 cut(s) 24, 152, 209, 267, 758, 920
GsuI CTGGAG 1 cut(s) 1568
HaeIII GGCC 5 cut(s) 106, 151, 966, 1020, 1397
HgaI GACGC 4 cut(s) 143, 480, 1181, 1282
Hin1II CATG 5 cut(s) 266, 568, 1025, 1050, 1405
HincII GTYRAC 1 cut(s) 1439
HindII GTYRAC 1 cut(s) 1439
HindIII AAGCTT 1 cut(s) 699
HinfI GANTC 2 cut(s) 584, 1177
HphI GGTGA 5 cut(s) 409, 568, 965, 1133, 1186
Hpy166II GTNNAC 2 cut(s) 89, 1439
Hpy188I TCNGA 3 cut(s) 816, 1330, 1412
Hpy188III TCNNGA 6 cut(s) 536, 932, 1076, 1133, 1181, 1547
Hpy8I GTNNAC 2 cut(s) 89, 1439
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 7 cut(s) 394, 450, 553, 868, 890, 929, 1306
HpyCH4III ACNGT 2 cut(s) 42, 335
HpyCH4IV ACGT 1 cut(s) 889
HpyCH4V TGCA 6 cut(s) 26, 266, 359, 760, 922, 999
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 925, 1040
HpyF3I CTNAG 2 cut(s) 602, 1101
HpySE526I ACGT 1 cut(s) 889
Hsp92II CATG 5 cut(s) 266, 568, 1025, 1050, 1405
Ksp22I TGATCA 2 cut(s) 259, 661
Kzo9I GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
LguI GCTCTTC 1 cut(s) 1576
LmnI GCTCC 6 cut(s) 201, 511, 538, 1050, 1412, 1549
Lsp1109I GCAGC 5 cut(s) 10, 220, 278, 744, 906
LweI GCATC 4 cut(s) 523, 1052, 1126, 1321
MabI ACCWGGT 1 cut(s) 573
MaeI CTAG 3 cut(s) 425, 1064, 1133
MaeII ACGT 1 cut(s) 889
MaeIII GTNAC 3 cut(s) 445, 465, 1059
MalI GATC 6 cut(s) 121, 261, 502, 663, 1317, 1327
MboI GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
MboII GAAGA 3 cut(s) 202, 292, 1593
MflI RGATCY 1 cut(s) 1325
MhlI GDGCHC 2 cut(s) 1096, 1167
MlsI TGGCCA 1 cut(s) 1020
MluNI TGGCCA 1 cut(s) 1020
MmeI TCCRAC 2 cut(s) 825, 839
Mox20I TGGCCA 1 cut(s) 1020
MroXI GAANNNNTTC 2 cut(s) 299, 439
MscI TGGCCA 1 cut(s) 1020
MseI TTAA 3 cut(s) 959, 1248, 1461
MslI CAYNNNNRTG 2 cut(s) 82, 1172
Msp20I TGGCCA 1 cut(s) 1020
MspCI CTTAAG 1 cut(s) 1460
MspR9I CCNGG 1 cut(s) 575
Mva1269I GAATGC 1 cut(s) 1522
MvaI CCWGG 1 cut(s) 575
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 6 cut(s) 14, 23, 103, 133, 925, 1040
NdeI CATATG 1 cut(s) 904
NdeII GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
NlaIII CATG 5 cut(s) 266, 568, 1025, 1050, 1405
NlaIV GGNNCC 3 cut(s) 197, 940, 1551
NmuCI GTSAC 1 cut(s) 465
NspI RCATGY 1 cut(s) 1405
OliI CACNNNNGTG 1 cut(s) 1172
PaeR7I CTCGAG 1 cut(s) 1095
PceI AGGCCT 2 cut(s) 106, 1397
PciSI GCTCTTC 1 cut(s) 1576
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PctI GAATGC 1 cut(s) 1522
PdmI GAANNNNTTC 2 cut(s) 299, 439
PfeI GAWTC 2 cut(s) 584, 1177
PkrI GCNGC 6 cut(s) 25, 153, 210, 268, 759, 921
Psp6I CCWGG 1 cut(s) 573
PspGI CCWGG 1 cut(s) 573
PspN4I GGNNCC 3 cut(s) 197, 940, 1551
PspPI GGNCC 2 cut(s) 149, 335
PspXI VCTCGAGB 1 cut(s) 1095
PsrI GAACNNNNNNTAC 2 cut(s) 883, 915
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 1325
RsaI GTAC 3 cut(s) 39, 1107, 1209
RsaNI GTAC 3 cut(s) 38, 1106, 1208
RseI CAYNNNNRTG 2 cut(s) 82, 1172
SapI GCTCTTC 1 cut(s) 1576
SaqAI TTAA 3 cut(s) 959, 1248, 1461
SatI GCNGC 6 cut(s) 24, 152, 209, 267, 758, 920
Sau3AI GATC 6 cut(s) 119, 259, 500, 661, 1315, 1325
Sau96I GGNCC 2 cut(s) 149, 335
ScaI AGTACT 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 575
SduI GDGCHC 2 cut(s) 1096, 1167
SexAI ACCWGGT 1 cut(s) 573
SfaNI GCATC 4 cut(s) 523, 1052, 1126, 1321
Sfr274I CTCGAG 1 cut(s) 1095
SinI GGWCC 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 1095
SmiI ATTTAAAT 1 cut(s) 960
SmiMI CAYNNNNRTG 2 cut(s) 82, 1172
SmlI CTYRAG 2 cut(s) 1095, 1460
SmoI CTYRAG 2 cut(s) 1095, 1460
SseBI AGGCCT 2 cut(s) 106, 1397
SsiI CCGC 2 cut(s) 152, 1202
SspI AATATT 2 cut(s) 342, 738
SspMI CTAG 3 cut(s) 425, 1064, 1133
StuI AGGCCT 2 cut(s) 106, 1397
StyD4I CCNGG 1 cut(s) 573
StyI CCWWGG 1 cut(s) 453
SwaI ATTTAAAT 1 cut(s) 960
TaaI ACNGT 2 cut(s) 42, 335
TaiI ACGT 1 cut(s) 892
TaqI TCGA 5 cut(s) 145, 494, 582, 1096, 1150
TatI WGTACW 2 cut(s) 37, 1207
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 2 cut(s) 584, 1177
Tru1I TTAA 3 cut(s) 959, 1248, 1461
Tru9I TTAA 3 cut(s) 959, 1248, 1461
TscAI CASTG 2 cut(s) 870, 1036
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 5 cut(s) 23, 208, 266, 757, 919
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 8 cut(s) 66, 342, 342, 584, 633, 698, 891, 1172
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 2 cut(s) 870, 1036
Vha464I CTTAAG 1 cut(s) 1460
VpaK11BI GGWCC 1 cut(s) 335
XagI CCTNNNNNAGG 1 cut(s) 551
XapI RAATTY 2 cut(s) 141, 1244
XbaI TCTAGA 1 cut(s) 1132
XceI RCATGY 1 cut(s) 1405
XhoI CTCGAG 1 cut(s) 1095
XmnI GAANNNNTTC 2 cut(s) 299, 439
XspI CTAG 3 cut(s) 425, 1064, 1133
ZrmI AGTACT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.