MD02G1265700.v1.1

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000439
Physical Location & Seq
Reverse (-)
31984152 .. 31988373
4222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1265700.v1.1.491

Sequence Viewer

Length: 2253 bp
ATGCCGCCAATGCCGAAGAAAAATCGAAAGATGGCGGCTGATGCCTGCCGTGCAATGAAGGAGTTTGGCATTTCGGAAGTTCGAACGAGGGCGGGGGTTAAGGAACTTCTGAAATTGTATGAGAACAACTGGGAGCTTATTGTGGAGGACAATCACAGAGTGCTGCTTGAGTTTTTATTGGAAGGTGACACTGACAACAACAAGGAAGAGGAAGAGGCAGAGGCAGACGAAGAGATAGCCGAACAAAAGGGCAAAGGAGTTTCGATATGTGAATCAAAGGCTGTGAAACGTAAGAACAAAGCAGCTCCATCTCATGGTAAAACTGAAGATGTTCAAATTCCAATCAAGAGATATTGCACAAGGCAACAGACAAAACAAGCATTGTCCCCTTTGAAATGCGCCACACCTTGCCTTGCAGAAACTCCATCAAAAATGGCACATCAGAATGAGGATTCTGAGGAATCTGAAGACTCTCAACCACTAGTGAGAAGAAGTCGCCGGAGACTCCAAGAGGGAGAGGTTTTAGACAGTGGGAATAACTTCAGTCCTGTATGTAATCGCAACACACAGCAGGAAGAAAGTCTCAGTATGATGTGTGAAGATAGTTGTTCAGACTTTGATGACGATGGCTTGAATTTCTTGGAAGTGAAATCATCCACTGTGGACAGTAGAGGAACTGTTTTAGATTTGTCACTGTTTGATGTTGAATCATCTCCTCCAAAGGGAGAGCTCGAAATGTCTTTGATTTGTCACCCTTCTCTACATTCTGATTTCGATCTTCCACATTCTGATGAAGCTCTAGAATTATTGGAAGAAACATGCATGAAATCGTACGGGGTCAGAGAATGCTTGGTGGAGACAGGATTTGATTCCACTTTTGATGAAAGTACAATTTCCAGAGATGTAGCAGAGTTAAAGAATTCTGATGCACAAGATGTTCTTGAATCTCATTATCAAGGCAATGATTGCAATACTCTAAGTTCCCCGAGCGAATTATTTGTATTTCAGAATCTTATCAAGGTCGTTCCCCATATACCCAAGCATATAGCTTTTGGTTGTTTTAATGATCTTCACCGCTTAATTGGTTTTTCTAGTACGGATATTGAGAGCATCTGTGGTGATTCGGCAAAGAGATTAGTGATCTTCCAAGGCCAGAAATCTCCAAAATTACGCAAGTTGGAGGAGGCTGCTCATGACTACCATTCTTCCATTGGAGTTATAAAATCCGGTGTTTATATTGAAGACATAACTAGAGGTGAGGAGAGAGTGAAAATTTCCTTGGAGGATGGAAAAAATGTTGAAGATCTGCCAAACTTTCTTTACATCCGTCAGAGTTTAGTTTATAAGAATGCCTCTGTGAAATTTTCACTTGGTCGTATGTCTCATGACGGTTGCTATTCACGTTGTTATGGGGATTGTCTGACATCACCCATGCCTTGTGTCTGTGCTTCTGAAACTGGGGGTCAGTTTGCTTACACACCAGGAGGATTGGTCAAAGAGATGTTTCTGGAAAAATGTATAGCCTTGAAGCGGGAACCTATACAACACCACTGCTTCTATTGTAGAGAGTGCCCTCTCGAAAAGTCTAAGTACGAGAAGAGTTCTGTGGCATGCAAAGGCCATTTGTTACATAAATTTATTAAGGAGTGTTGGCATACATGTGGATGCAATAAGAACTGTGGAAACCGGATTGTTCAGCAAGGCATCAGCGGGAAGTTGCAGGTATTTTTGACACCTGAAGGGAAAGGATGGGGTCTTCGAACACTGGAGGACTTGCCAAGAGGAGCTTTTGTTTGTGAGTATGTTGGAGAGATATTAACCAACACAGAACTACATGAGCGAAATGTGCCAAGTACTGGTAAGAAGCATGCATACTACGCAGTGCCACTAGATGCGGGCTGGGGTGTGAAGGGTGTCTTGAAAGATGAAGAGGCACTTTGCTTGGACGCTACTGTTTATGGAAATGTTGCAAGATTTATCAATCACAGATGTTCTGATGCTACCTTGGTTGAGATTCCTGTTGAAGTGGAGACCCCGGATCATCACTATTATCATCTTGCCCTTTTCACAACAAGAAACATTGCTGCTATGGAGGAGCTCACTTGGGACTATGGCATTGACTTTCGTGAGCGTGATCATCCTAGGACTGCGTTTCAATGTCTCTGTGGGAGCCCACTCTGCCGTGATCGTAAGGAGTCCAAGGGTGACGTGCAGCTACACACTTACGCTCGACGAAGACGATGCAGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SET WIYLD

Protein Analysis

751

Amino Acids

84.58

Weight (kDa)

5.62

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 7 - 61 1.6e-14 Ubiquitin-binding WIYLD domain
SET PF00856 583 - 705 2.1e-19 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000439)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380
fragaria_vesca FvH4_2g02250 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_5g31170 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620
malus_domestica MD02G1265700.v1.1 MD04G1231900.v1.1 MD12G1043600.v1.1 MD12G1250000.v1.1
prunus_persica Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1
pyrus_communis pycom02g22720 pycom04g20520
rosa_chinensis RchiOBHm_Chr3g0449441 RchiOBHm_Chr4g0395181 RchiOBHm_Chr6g0245941 RchiOBHm_Chr7g0228041
rosa_laevigata RLG00000001593 RLG00000009641 RLG00000015284 RLG00000025830
rosa_multiflora Rmu_co8250301.1_g000001 Rmu_co8518901.1_g000001 Rmu_sc0004858.1_g000011 Rmu_sc0007224.1_g000005 Rmu_sc0007891.1_g000010 Rmu_sc0019478.1_g000002 Rmu_sc0024829.1_g000001 Rmu_sc0039188.1_g000001
rosa_roxburghii Rroxscaffold_3G00231620 Rroxscaffold_5G00340120 Rroxscaffold_6G00427390 Rroxscaffold_7G00215050
rosa_rugosa Rorug02G0620600 Rorug03G0359100.1 Rorug03G0359200.1 Rorug03G0359300.1 Rorug05G0517600 Rorug05G0517700 Rorug05G0517800 Rorug05G0517900 Rorug05G0518000 Rorug05G0518100 Rorug05G0518200 Rorug05G0518300.1 Rorug07G0245600
rosa_samantha Rh3AG018400 Rh3BG018800 Rh3CG017700 Rh4AG057300 Rh4BG056200 Rh4CG062300 Rh4DG053200 Rh6AG032500 Rh6BG028300 Rh6CG028300 Rh6CG028500 Rh6CG028600 Rh7AG399400 Rh7BG380800 Rh7CG417400 Rh7DG394900
rosa_wichuraiana Rw4G004610 Rw6G002670 Rw7G033190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1220, 1344
Acc36I ACCTGC 1 cut(s) 1711
AccB7I CCANNNNNTGG 2 cut(s) 314, 1856
AciI CCGC 7 cut(s) 5, 35, 92, 1075, 1531, 1710, 1895
AclWI GGATC 1 cut(s) 2046
AcsI RAATTY 6 cut(s) 336, 634, 919, 1272, 1361, 1634
AcuI CTGAAG 4 cut(s) 345, 486, 526, 1758
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 5 cut(s) 833, 889, 1096, 1592, 1855
AfiI CCNNNNNNNGG 4 cut(s) 314, 722, 1530, 1856
AflIII ACRYGT 1 cut(s) 1658
AhlI ACTAGT 1 cut(s) 481
AjiI CACGTC 1 cut(s) 2209
AjnI CCWGG 1 cut(s) 1480
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
AluBI AGCT 8 cut(s) 136, 305, 730, 797, 1049, 1787, 2098, 2215
AluI AGCT 8 cut(s) 136, 305, 730, 797, 1049, 1787, 2098, 2215
Alw21I GWGCWC 2 cut(s) 732, 2100
Alw26I GTCTC 6 cut(s) 496, 587, 851, 1386, 2024, 2165
AlwI GGATC 1 cut(s) 2046
Ama87I CYCGRG 1 cut(s) 985
AoxI GGCC 2 cut(s) 1150, 1618
ApeKI GCWGC 5 cut(s) 163, 302, 1187, 2084, 2212
ApoI RAATTY 6 cut(s) 336, 634, 919, 1272, 1361, 1634
Asp700I GAANNNNTTC 2 cut(s) 330, 539
AspA2I CCTAGG 1 cut(s) 2141
AspLEI GCGC 1 cut(s) 401
AsuC2I CCSGG 1 cut(s) 2036
AsuHPI GGTGA 7 cut(s) 197, 743, 1064, 1130, 1268, 1419, 2216
AsuII TTCGAA 2 cut(s) 82, 1759
AvaI CYCGRG 1 cut(s) 985
AvrII CCTAGG 1 cut(s) 2141
BaeGI GKGCMC 1 cut(s) 1574
BanII GRGCYC 3 cut(s) 732, 2100, 2174
BarI GAAGNNNNNNTAC 2 cut(s) 1856, 1888
BbsI GAAGAC 4 cut(s) 474, 1248, 1748, 2242
Bbv12I GWGCWC 2 cut(s) 732, 2100
BbvI GCAGC 5 cut(s) 150, 314, 1174, 2071, 2224
BccI CCATC 6 cut(s) 25, 316, 433, 620, 1280, 1743
BceAI ACGGC 2 cut(s) 33, 2166
BcgI CGANNNNNNTGC 1 cut(s) 2223
BciT130I CCWGG 1 cut(s) 1482
BclI TGATCA 1 cut(s) 2134
BcnI CCSGG 1 cut(s) 2036
BcoDI GTCTC 6 cut(s) 496, 587, 851, 1386, 2024, 2165
BcuI ACTAGT 1 cut(s) 481
BfaI CTAG 6 cut(s) 482, 800, 1092, 1251, 1889, 2142
BfuAI ACCTGC 1 cut(s) 1711
BglII AGATCT 1 cut(s) 1303
BisI GCNGC 7 cut(s) 5, 36, 164, 303, 1188, 2085, 2213
BlnI CCTAGG 1 cut(s) 2141
BlsI GCNGC 7 cut(s) 6, 37, 165, 304, 1189, 2086, 2214
BmcAI AGTACT 1 cut(s) 1855
Bme1390I CCNGG 2 cut(s) 1482, 2036
BmeT110I CYCGRG 1 cut(s) 985
BmgBI CACGTC 1 cut(s) 2209
BmiI GGNNCC 2 cut(s) 1536, 2171
BmrFI CCNGG 2 cut(s) 1482, 2036
BmrI ACTGGG 2 cut(s) 139, 1467
BmsI GCATC 8 cut(s) 31, 916, 1119, 1655, 1713, 1882, 1987, 2231
BmuI ACTGGG 2 cut(s) 139, 1467
BpiI GAAGAC 4 cut(s) 474, 1248, 1748, 2242
BpmI CTGGAG 1 cut(s) 1787
Bpu14I TTCGAA 2 cut(s) 82, 1759
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 2036
BsaBI GATNNNNATC 1 cut(s) 774
BsaI GGTCTC 1 cut(s) 2024
BsaJI CCNNGG 6 cut(s) 1147, 1278, 2004, 2034, 2141, 2199
BsaWI WCCGGW 2 cut(s) 1226, 1686
BsaXI ACNNNNNCTCC 4 cut(s) 1476, 1506, 1776, 1806
Bsc4I CCNNNNNNNGG 4 cut(s) 314, 722, 1530, 1856
Bse1I ACTGG 4 cut(s) 134, 1462, 1770, 1861
Bse3DI GCAATG 3 cut(s) 60, 967, 2079
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 1 cut(s) 1482
BseDI CCNNGG 6 cut(s) 1147, 1278, 2004, 2034, 2141, 2199
BseGI GGATG 6 cut(s) 653, 1291, 1323, 1670, 1754, 2137
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 4 cut(s) 314, 722, 1530, 1856
BseMI GCAATG 3 cut(s) 60, 967, 2079
BseMII CTCAG 2 cut(s) 447, 598
BseNI ACTGG 4 cut(s) 134, 1462, 1770, 1861
BseRI GAGGAG 5 cut(s) 705, 1196, 1274, 1797, 2108
BseSI GKGCMC 1 cut(s) 1574
BseXI GCAGC 5 cut(s) 150, 314, 1174, 2071, 2224
BseYI CCCAGC 1 cut(s) 1899
BsgI GTGCAG 1 cut(s) 2231
BshFI GGCC 2 cut(s) 1152, 1620
BsiHKAI GWGCWC 2 cut(s) 732, 2100
BsiHKCI CYCGRG 1 cut(s) 985
BsiSI CCGG 4 cut(s) 499, 1227, 1687, 2036
BsiWI CGTACG 1 cut(s) 831
BslFI GGGAC 2 cut(s) 370, 2120
BslI CCNNNNNNNGG 4 cut(s) 314, 722, 1530, 1856
BsmAI GTCTC 6 cut(s) 496, 587, 851, 1386, 2024, 2165
BsmFI GGGAC 2 cut(s) 370, 2120
BsmI GAATGC 2 cut(s) 851, 1354
BsnI GGCC 2 cut(s) 1152, 1620
Bso31I GGTCTC 1 cut(s) 2024
BsoBI CYCGRG 1 cut(s) 985
Bsp119I TTCGAA 2 cut(s) 82, 1759
Bsp1286I GDGCHC 4 cut(s) 732, 1574, 2100, 2174
Bsp143I GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
BspACI CCGC 7 cut(s) 5, 35, 92, 1075, 1531, 1710, 1895
BspANI GGCC 2 cut(s) 1152, 1620
BspCNI CTCAG 2 cut(s) 448, 597
BspHI TCATGA 2 cut(s) 1192, 1384
BspLI GGNNCC 2 cut(s) 1536, 2171
BspMI ACCTGC 1 cut(s) 1711
BspPI GGATC 1 cut(s) 2046
BspT104I TTCGAA 2 cut(s) 82, 1759
BspTNI GGTCTC 1 cut(s) 2024
BsrDI GCAATG 3 cut(s) 60, 967, 2079
BsrI ACTGG 4 cut(s) 134, 1462, 1770, 1861
BssECI CCNNGG 6 cut(s) 1147, 1278, 2004, 2034, 2141, 2199
BssMI GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
BssT1I CCWWGG 5 cut(s) 1147, 1278, 2004, 2141, 2199
Bst2UI CCWGG 1 cut(s) 1482
Bst4CI ACNGT 8 cut(s) 530, 661, 668, 679, 696, 1391, 1679, 1954
Bst6I CTCTTC 5 cut(s) 201, 207, 225, 1592, 1923
BstAPI GCANNNNNTGC 1 cut(s) 966
BstBI TTCGAA 2 cut(s) 82, 1759
BstC8I GCNNGC 4 cut(s) 46, 1612, 1869, 1897
BstDEI CTNAG 4 cut(s) 456, 584, 977, 1587
BstF5I GGATG 6 cut(s) 653, 1291, 1323, 1670, 1754, 2137
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 7 cut(s) 778, 1069, 1143, 1306, 2041, 2137, 2188
BstMAI GTCTC 6 cut(s) 496, 587, 851, 1386, 2024, 2165
BstMBI GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
BstMWI GCNNNNNNNGC 7 cut(s) 10, 41, 50, 966, 1846, 1877, 2178
BstNI CCWGG 1 cut(s) 1482
BstNSI RCATGY 4 cut(s) 822, 1614, 1662, 1871
BstSCI CCNGG 2 cut(s) 1480, 2034
BstSLI GKGCMC 1 cut(s) 1574
BstV1I GCAGC 5 cut(s) 150, 314, 1174, 2071, 2224
BstV2I GAAGAC 4 cut(s) 474, 1248, 1748, 2242
BstX2I RGATCY 1 cut(s) 1303
BstYI RGATCY 1 cut(s) 1303
BsuRI GGCC 2 cut(s) 1152, 1620
BtrI CACGTC 1 cut(s) 2209
BtsCI GGATG 6 cut(s) 653, 1291, 1323, 1670, 1754, 2137
BtsI GCAGTG 2 cut(s) 1549, 1887
BtsIMutI CAGTG 7 cut(s) 189, 535, 657, 692, 1549, 1763, 1887
BveI ACCTGC 1 cut(s) 1711
Cac8I GCNNGC 4 cut(s) 46, 1612, 1869, 1897
CciI TCATGA 2 cut(s) 1192, 1384
CfoI GCGC 1 cut(s) 401
CseI GACGC 1 cut(s) 1955
Csp6I GTAC 5 cut(s) 832, 888, 1095, 1591, 1854
CviQI GTAC 5 cut(s) 832, 888, 1095, 1591, 1854
DdeI CTNAG 4 cut(s) 456, 584, 977, 1587
DpnI GATC 7 cut(s) 777, 1068, 1142, 1305, 2040, 2136, 2187
DpnII GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
DraIII CACNNNGTG 1 cut(s) 160
Eam1104I CTCTTC 5 cut(s) 201, 207, 225, 1592, 1923
EarI CTCTTC 5 cut(s) 201, 207, 225, 1592, 1923
Ecl136II GAGCTC 2 cut(s) 730, 2098
Eco130I CCWWGG 5 cut(s) 1147, 1278, 2004, 2141, 2199
Eco24I GRGCYC 3 cut(s) 732, 2100, 2174
Eco31I GGTCTC 1 cut(s) 2024
Eco53kI GAGCTC 2 cut(s) 730, 2098
Eco57I CTGAAG 4 cut(s) 345, 486, 526, 1758
Eco88I CYCGRG 1 cut(s) 985
EcoICRI GAGCTC 2 cut(s) 730, 2098
EcoRI GAATTC 1 cut(s) 919
EcoRII CCWGG 1 cut(s) 1480
EcoT14I CCWWGG 5 cut(s) 1147, 1278, 2004, 2141, 2199
EcoT22I ATGCAT 2 cut(s) 824, 1873
EcoT38I GRGCYC 3 cut(s) 732, 2100, 2174
ErhI CCWWGG 5 cut(s) 1147, 1278, 2004, 2141, 2199
FalI AAGNNNNNCTT 8 cut(s) 924, 956, 1771, 1803, 1901, 1933, 1920, 1952
FaqI GGGAC 2 cut(s) 370, 2120
FauI CCCGC 4 cut(s) 85, 1524, 1703, 1888
FbaI TGATCA 1 cut(s) 2134
Fnu4HI GCNGC 7 cut(s) 5, 36, 164, 303, 1188, 2085, 2213
FokI GGATG 6 cut(s) 640, 1298, 1310, 1677, 1761, 2124
FriOI GRGCYC 3 cut(s) 732, 2100, 2174
Fsp4HI GCNGC 7 cut(s) 5, 36, 164, 303, 1188, 2085, 2213
FspBI CTAG 6 cut(s) 482, 800, 1092, 1251, 1889, 2142
GlaI GCGC 1 cut(s) 400
GluI GCNGC 7 cut(s) 5, 36, 164, 303, 1188, 2085, 2213
GsaI CCCAGC 1 cut(s) 1903
GsuI CTGGAG 1 cut(s) 1787
HaeIII GGCC 2 cut(s) 1152, 1620
HapII CCGG 4 cut(s) 499, 1227, 1687, 2036
HgaI GACGC 1 cut(s) 1955
HhaI GCGC 1 cut(s) 401
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HpaII CCGG 4 cut(s) 499, 1227, 1687, 2036
HphI GGTGA 7 cut(s) 197, 743, 1064, 1130, 1268, 1419, 2216
Hpy166II GTNNAC 1 cut(s) 664
Hpy8I GTNNAC 1 cut(s) 664
Hpy99I CGWCG 1 cut(s) 2235
HpyAV CCTTC 5 cut(s) 52, 176, 765, 1733, 1903
HpyCH4III ACNGT 8 cut(s) 530, 661, 668, 679, 696, 1391, 1679, 1954
HpyCH4IV ACGT 3 cut(s) 289, 1402, 2208
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 41, 50, 966, 1846, 1877, 2178
HpyF3I CTNAG 4 cut(s) 456, 584, 977, 1587
HpySE526I ACGT 3 cut(s) 289, 1402, 2208
HspAI GCGC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 2134
Kzo9I GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
LmnI GCTCC 5 cut(s) 133, 310, 1784, 2095, 2169
Lsp1109I GCAGC 5 cut(s) 150, 314, 1174, 2071, 2224
LweI GCATC 8 cut(s) 31, 916, 1119, 1655, 1713, 1882, 1987, 2231
MaeI CTAG 6 cut(s) 482, 800, 1092, 1251, 1889, 2142
MaeII ACGT 3 cut(s) 289, 1402, 2208
MaeIII GTNAC 5 cut(s) 185, 690, 749, 1626, 2204
MalI GATC 7 cut(s) 777, 1068, 1142, 1305, 2040, 2136, 2187
MboI GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
MflI RGATCY 1 cut(s) 1303
MhlI GDGCHC 4 cut(s) 732, 1574, 2100, 2174
MlyI GAGTC 3 cut(s) 464, 498, 2204
MmeI TCCRAC 2 cut(s) 1158, 1786
Mph1103I ATGCAT 2 cut(s) 824, 1873
MroXI GAANNNNTTC 2 cut(s) 330, 539
MseI TTAA 6 cut(s) 99, 914, 1062, 1079, 1641, 1817
MslI CAYNNNNRTG 4 cut(s) 444, 789, 1659, 1663
MspA1I CMGCKG 1 cut(s) 1710
MspI CCGG 4 cut(s) 499, 1227, 1687, 2036
MspR9I CCNGG 2 cut(s) 1482, 2036
Mva1269I GAATGC 2 cut(s) 851, 1354
MvaI CCWGG 1 cut(s) 1482
MwoI GCNNNNNNNGC 7 cut(s) 10, 41, 50, 966, 1846, 1877, 2178
NciI CCSGG 1 cut(s) 2036
NdeII GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
NlaIV GGNNCC 2 cut(s) 1536, 2171
NmuCI GTSAC 4 cut(s) 185, 690, 749, 2204
NsiI ATGCAT 2 cut(s) 824, 1873
NspI RCATGY 4 cut(s) 822, 1614, 1662, 1871
NspV TTCGAA 2 cut(s) 82, 1759
PaeI GCATGC 2 cut(s) 1614, 1871
PagI TCATGA 2 cut(s) 1192, 1384
PciI ACATGT 1 cut(s) 1658
PcsI WCGNNNNNNNCGW 1 cut(s) 2236
PctI GAATGC 2 cut(s) 851, 1354
PdmI GAANNNNTTC 2 cut(s) 330, 539
PfeI GAWTC 9 cut(s) 272, 452, 461, 707, 869, 944, 1009, 1121, 2014
Pfl23II CGTACG 1 cut(s) 831
PflMI CCANNNNNTGG 2 cut(s) 314, 1856
PkrI GCNGC 7 cut(s) 6, 37, 165, 304, 1189, 2086, 2214
PleI GAGTC 3 cut(s) 464, 498, 2203
PpsI GAGTC 3 cut(s) 464, 498, 2203
PscI ACATGT 1 cut(s) 1658
PsiI TTATAA 2 cut(s) 1220, 1344
Psp124BI GAGCTC 2 cut(s) 732, 2100
Psp6I CCWGG 1 cut(s) 1480
PspFI CCCAGC 1 cut(s) 1899
PspGI CCWGG 1 cut(s) 1480
PspLI CGTACG 1 cut(s) 831
PspN4I GGNNCC 2 cut(s) 1536, 2171
PsuI RGATCY 1 cut(s) 1303
RsaI GTAC 5 cut(s) 833, 889, 1096, 1592, 1855
RsaNI GTAC 5 cut(s) 832, 888, 1095, 1591, 1854
RseI CAYNNNNRTG 4 cut(s) 444, 789, 1659, 1663
SacI GAGCTC 2 cut(s) 732, 2100
SaqAI TTAA 6 cut(s) 99, 914, 1062, 1079, 1641, 1817
SatI GCNGC 7 cut(s) 5, 36, 164, 303, 1188, 2085, 2213
Sau3AI GATC 7 cut(s) 775, 1066, 1140, 1303, 2038, 2134, 2185
ScaI AGTACT 1 cut(s) 1855
SchI GAGTC 3 cut(s) 464, 498, 2204
ScrFI CCNGG 2 cut(s) 1482, 2036
SduI GDGCHC 4 cut(s) 732, 1574, 2100, 2174
SfaNI GCATC 8 cut(s) 31, 916, 1119, 1655, 1713, 1882, 1987, 2231
SfuI TTCGAA 2 cut(s) 82, 1759
SmiMI CAYNNNNRTG 4 cut(s) 444, 789, 1659, 1663
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 481
SphI GCATGC 2 cut(s) 1614, 1871
SsiI CCGC 7 cut(s) 5, 35, 92, 1075, 1531, 1710, 1895
SspMI CTAG 6 cut(s) 482, 800, 1092, 1251, 1889, 2142
SstI GAGCTC 2 cut(s) 732, 2100
StyD4I CCNGG 2 cut(s) 1480, 2034
StyI CCWWGG 5 cut(s) 1147, 1278, 2004, 2141, 2199
TaaI ACNGT 8 cut(s) 530, 661, 668, 679, 696, 1391, 1679, 1954
TaiI ACGT 3 cut(s) 292, 1405, 2211
TaqI TCGA 8 cut(s) 25, 82, 263, 732, 774, 1578, 1759, 2230
TatI WGTACW 2 cut(s) 887, 1853
TauI GCSGC 2 cut(s) 7, 38
TfiI GAWTC 9 cut(s) 272, 452, 461, 707, 869, 944, 1009, 1121, 2014
Tru1I TTAA 6 cut(s) 99, 914, 1062, 1079, 1641, 1817
Tru9I TTAA 6 cut(s) 99, 914, 1062, 1079, 1641, 1817
TscAI CASTG 7 cut(s) 196, 535, 664, 699, 1556, 1770, 1887
TseFI GTSAC 4 cut(s) 185, 690, 749, 2204
TseI GCWGC 5 cut(s) 163, 302, 1187, 2084, 2212
Tsp45I GTSAC 4 cut(s) 185, 690, 749, 2204
TspDTI ATGAA 5 cut(s) 71, 807, 839, 897, 1941
TspGWI ACGGA 2 cut(s) 1112, 1316
TspRI CASTG 7 cut(s) 196, 535, 664, 699, 1556, 1770, 1887
Van91I CCANNNNNTGG 2 cut(s) 314, 1856
XapI RAATTY 6 cut(s) 336, 634, 919, 1272, 1361, 1634
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 4 cut(s) 822, 1614, 1662, 1871
XcmI CCANNNNNNNNNTGG 1 cut(s) 1208
XmaJI CCTAGG 1 cut(s) 2141
XmnI GAANNNNTTC 2 cut(s) 330, 539
XspI CTAG 6 cut(s) 482, 800, 1092, 1251, 1889, 2142
ZrmI AGTACT 1 cut(s) 1855
Zsp2I ATGCAT 2 cut(s) 824, 1873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.