Rw7G033190

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000439
Physical Location & Seq
Reverse (-)
48774963 .. 48780511
5549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G033190.1

Sequence Viewer

Length: 3078 bp
ATGTGGCCGAAAGGTAGCAAAATTGCAAAGGCCTGTCGGGCAATGAAAGAATTTGGCATTGCTGAAGTCCAAACAAAGGCTGCGCTGAAGCAACTTCTGGAAGTGTATGAAAATAACTGGGATTTCATTGAAGCTGATGATTACAAAGAACTCTTTAATTTTATTGTACCAGATAACAAGAAGCCAAATGAAGAGAAAGTGCAGCCCAAGGTAAAAGGCGCTTTGATGCGTGAATCAAAGAGAGTGAATTCTAAGAACAAACAAGCTCCATCATATGATACAACTGAAGTTATTGAGCGTCCAACCAAGAGATATTGCACCAGGCAACAGAAAAATCAGCCATTATCTTGTACCAAATACTCTGAATCAAGCTTGAGAAGAACTCCACCAGAAAGTATGCATATTAAGTTTGATGAAGAATCTGAGGAATCTGAGGAGCGGCAGCCATTAGTGAGAAAACCTCGACAAAGGCACACAAATCCTCAAGAAGTTTGGGTTGCGGATAATAAGGTTGGTCCTGTGCCTGATGACTGTCCAGAACTTGATACAAGTCCGAATCTTATATGTGAAGGTAACTTCTCAGATTCTGATGGTTGTGATGTTGGTGAAGATGTGATGAAGTACCTGCAACTGAAATCTAGGAACAAACAACCTCCATCATATGATACACCTGAAGTTATTGAGTGGCAAACCAAGAGATTTCGCACCAGGCAACAGAAAAATCAGCCTTCATCTCTTACCAAATACTCTGAATCAAGCTTGAGAAGAACTCCAGGAAAAATGCACGTGAAGTTTGATGAAGAATCTGAGGAATCTGAGGACTCGCAGCCATTAGTGAGAAAACCTCGACAAAGACACATAAATCCACAAGAAGTTTGGGTCACTAGGAATAACATTGATGATGTGCTTGATGACTGTCCAGAACTTGAAATAGGTCCCAGTCCTGTATGTGAAGGTAACTTCACAAATTCTGATGGTTGTGATGTTGATGAAGATGTGGTGAAGTACCTGCCAGTGGAATTATTGACTGAGAGCAATAGTGTGAGTGTCTCAGATTGGTCTCAGACTGATGGTGCATCTTCTTCCCCAGTGAAAAATCCTTTGATTTCTAGCTCTTCGCAAAAGTCTGGTTTTGATCTTCCTAACGGTGAAGTATCCCAAGAAATGATGGAAGAGAAATGCATAAAATCATATAGGACTGGAGAATGCCACTCCATGGCGATAGTGACTAATGTCACTGAGAATAAGCCAGAATTAATAACTTCTAATGAACAAGATCAAGGCAGTAAGTGCAGTGGTCTGGGTTCTTTTAACGAGCTATTTGTTGATCAGAATCTTATCAAGCCTATACGTATACCTAATTATGGAGCGTTGGGTAGTTTTGAAGGTCTTTGCAGCTTAATTGGTATTAAAGTTAAGGATATGGAGAACATCTGTGGGGAAAGTGCAAAGAGATCAAGGGTTTCCAAAGGTCAAAAAACATCACAATTATGCAAGGTGGAAGCCGGTCATAACTACCATTCTTCACTTAGAGTTACAGAGTCTGCATTTAACATTGATGACATAACGAGAGGTGAAGAAAGATTTAAGATTTCCTTGGAAAATGGAAGAAATGCTGAGTTGCCAACCTTTTCTTACATCGCGCAGAATTTAATCTATAATAAGGCCTCTGTGAGATTTGCACTTGCTAATATATCCGATGAGGATTGCTGTTCAAATTGTTATGGGGACTGTCTTACATCATCAATTCCTTGTCTTTGTGTGATTGCCAATGGGGGTGAATGTGCTTACAAGCCAGGAGGATTGGTCAAGGAGAAGTTTCTGGAAGAATGTATATCTATGAAGCGTGAGCCTGAAAAGCACCCATACTTCTACTGTAAAAAGTGCCCACTAGTAAGGTCAGAGAATGCGAACAGTTCTGTGGCATGCAAGGGACATTTGTTACGGAGGTTTATAAAGGAATGCTCTTATAAATGCGGTTGTAACAGGAGCTGTGGAAACCGGATTGTACAGAAAGGCATTGCTGTGAAATTGCAGGTATTTTTGACACCTGAAGGGAAAGGGTGGGGTCTAAGAACACTGGAGGCATTGCCAAGAGGGGCTTTTGTTTGTGAATATGTTGGAGAGATAGTAACCATCGACGAACTACATGAGCGAAGTTTGCTGAGTATTGGTAAAGAGCATACCTACCCAGTACTACTAGATGCAGACTGGGGTTCACAGGATGTGTTGAAAGATGAAAAGGCTCTTTGCTTGGACACTACAGTCTACGGAAATGTTGCAAGATTCATTAACCACAGATGTTCTGATGCTACATTGGTCGAGATCCCAGTTGAAGTGGAGACCCCAGATCGTCACTATTATCATGTTGCCCTTTTCACAACAAGGGATGTTGGTGCTATGGAGGAGCTCACTTGGGACTATGGCATTTCCTTTCATGACCATAATTATCCTGTGAAGCATTTCCGATGTCTCTGTGGAAGTCCATTATGTCGTGACCGCAAAGATATAAAAGGCAAAATTACATATGTCCGACGACGGCGTAAGGACGGGAGGGGTAACAACAAGGATGGTTTGCTTGATCACTTGGCAAAGGATCTTGCTACGGGTACACTGAAAGAGGAAATACAAGTAGAACCTGATTCATTGATGCAGCTTGAAGTTGTCCCGTCTACAGGTGCATTGAGGGAGGAGGTACAAGTAGCACCCCATGCATCCAGGCAGCTCGAGATTGTCCCATCTACGGGTGCGCCCAAAGCAGAGATACAAGTCGCTTCCGATCCATGCATGCAGCTTCAGGTTAACCCTTCTACTCCTAGAATTGAAGCCAGGAAGGCATTGCACTGTTTAAATTGGGAGCCAGTTAGGGATAGCTGCTCCTCTTCTTCCACTATGTATCTGGCCAAGGAGGTAGCAGACTTGCGGGCTCAGCTTGAGGCTGAGAAGGCAGCTCGAGAGAAGGACCAGATAAACTTTGAGGCTCATCGTGCTCAATTTCAGGCAGTGATGCAGCAACTATCTACGCTTCTCCCTGGGTTTCAGGTACCACAAGCTTCTGTTCAGATGCAGCTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SET WIYLD

Protein Analysis

1025

Amino Acids

115.52

Weight (kDa)

5.83

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 6 - 55 1.9e-15 Ubiquitin-binding WIYLD domain
SET PF00856 688 - 809 8.5e-18 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000439)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380
fragaria_vesca FvH4_2g02250 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_5g31170 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620
malus_domestica MD02G1265700.v1.1 MD04G1231900.v1.1 MD12G1043600.v1.1 MD12G1250000.v1.1
prunus_persica Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1
pyrus_communis pycom02g22720 pycom04g20520
rosa_chinensis RchiOBHm_Chr3g0449441 RchiOBHm_Chr4g0395181 RchiOBHm_Chr6g0245941 RchiOBHm_Chr7g0228041
rosa_laevigata RLG00000001593 RLG00000009641 RLG00000015284 RLG00000025830
rosa_multiflora Rmu_co8250301.1_g000001 Rmu_co8518901.1_g000001 Rmu_sc0004858.1_g000011 Rmu_sc0007224.1_g000005 Rmu_sc0007891.1_g000010 Rmu_sc0019478.1_g000002 Rmu_sc0024829.1_g000001 Rmu_sc0039188.1_g000001
rosa_roxburghii Rroxscaffold_3G00231620 Rroxscaffold_5G00340120 Rroxscaffold_6G00427390 Rroxscaffold_7G00215050
rosa_rugosa Rorug02G0620600 Rorug03G0359100.1 Rorug03G0359200.1 Rorug03G0359300.1 Rorug05G0517600 Rorug05G0517700 Rorug05G0517800 Rorug05G0517900 Rorug05G0518000 Rorug05G0518100 Rorug05G0518200 Rorug05G0518300.1 Rorug07G0245600
rosa_samantha Rh3AG018400 Rh3BG018800 Rh3CG017700 Rh4AG057300 Rh4BG056200 Rh4CG062300 Rh4DG053200 Rh6AG032500 Rh6BG028300 Rh6CG028300 Rh6CG028500 Rh6CG028600 Rh7AG399400 Rh7BG380800 Rh7CG417400 Rh7DG394900
rosa_wichuraiana Rw4G004610 Rw6G002670 Rw7G033190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1955, 1971
AasI GACNNNNNNGTC 1 cut(s) 2264
Acc36I ACCTGC 3 cut(s) 633, 1017, 2026
Acc65I GGTACC 1 cut(s) 3043
AccB1I GGYRCC 1 cut(s) 3043
AccB7I CCANNNNNTGG 1 cut(s) 1216
AccBSI CCGCTC 1 cut(s) 439
AccI GTMKAC 3 cut(s) 1354, 2268, 2672
AccII CGCG 1 cut(s) 1643
AciI CCGC 5 cut(s) 439, 500, 1977, 2500, 2923
AclWI GGATC 3 cut(s) 2320, 2604, 2774
AcoI YGGCCR 2 cut(s) 5, 2901
AcsI RAATTY 4 cut(s) 50, 247, 967, 1648
AcuI CTGAAG 6 cut(s) 84, 107, 306, 693, 2073, 2780
AcvI CACGTG 1 cut(s) 787
AfaI GTAC 9 cut(s) 168, 352, 623, 1007, 2010, 2196, 2611, 2697, 3045
AfiI CCNNNNNNNGG 7 cut(s) 76, 1015, 1216, 1364, 2675, 2743, 2744
AgsI TTSAA 8 cut(s) 131, 929, 1385, 1716, 2233, 2336, 2660, 2825
AhlI ACTAGT 1 cut(s) 1891
AjnI CCWGG 7 cut(s) 320, 707, 772, 1795, 2717, 2828, 3031
Alw21I GWGCWC 2 cut(s) 2412, 2992
Alw26I GTCTC 4 cut(s) 1054, 1065, 2336, 2477
AlwI GGATC 3 cut(s) 2320, 2604, 2774
AlwNI CAGNNNCTG 3 cut(s) 587, 1544, 1992
Ama87I CYCGRG 3 cut(s) 2726, 2952, 3071
AoxI GGCC 4 cut(s) 5, 30, 1665, 2901
ApoI RAATTY 4 cut(s) 50, 247, 967, 1648
AseI ATTAAT 1 cut(s) 1256
Asp700I GAANNNNTTC 1 cut(s) 2462
Asp718I GGTACC 1 cut(s) 3043
AspLEI GCGC 4 cut(s) 85, 221, 1645, 2752
AspS9I GGNCC 3 cut(s) 515, 935, 2962
AsuHPI GGTGA 5 cut(s) 617, 1012, 1160, 1586, 1790
AvaI CYCGRG 3 cut(s) 2726, 2952, 3071
AvaII GGWCC 3 cut(s) 515, 935, 2962
BaeGI GKGCMC 1 cut(s) 1889
BalI TGGCCA 1 cut(s) 2903
BanI GGYRCC 1 cut(s) 3043
BanII GRGCYC 2 cut(s) 2412, 2929
BbrPI CACGTG 1 cut(s) 787
Bbv12I GWGCWC 2 cut(s) 2412, 2992
BccI CCATC 9 cut(s) 277, 584, 664, 968, 1064, 1162, 2144, 2564, 2746
BceAI ACGGC 1 cut(s) 2555
BciT130I CCWGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
BciVI GTATCC 1 cut(s) 1165
BclI TGATCA 2 cut(s) 1327, 2581
BcoDI GTCTC 4 cut(s) 1054, 1065, 2336, 2477
BcuI ACTAGT 1 cut(s) 1891
BfaI CTAG 6 cut(s) 639, 885, 1110, 1892, 2201, 2817
BfmI CTRYAG 2 cut(s) 2262, 2673
BfoI RGCGCY 1 cut(s) 222
BfuAI ACCTGC 3 cut(s) 633, 1017, 2026
BfuI GTATCC 1 cut(s) 1165
BglI GCCNNNNNGGC 2 cut(s) 38, 2834
BlpI GCTNAGC 1 cut(s) 2928
BmcAI AGTACT 1 cut(s) 2196
Bme1390I CCNGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
Bme18I GGWCC 3 cut(s) 515, 935, 2962
BmeT110I CYCGRG 3 cut(s) 2726, 2952, 3071
BmgT120I GGNCC 3 cut(s) 515, 935, 2962
BmiI GGNNCC 3 cut(s) 937, 2859, 3045
BmrFI CCNGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
BmrI ACTGGG 6 cut(s) 127, 933, 1082, 2186, 2221, 2324
BmsI GCATC 8 cut(s) 216, 1085, 2194, 2299, 2640, 2723, 2997, 3054
BmuI ACTGGG 6 cut(s) 127, 933, 1082, 2186, 2221, 2324
BoxI GACNNNNGTC 1 cut(s) 1232
BplI GAGNNNNNCTC 8 cut(s) 367, 399, 445, 477, 754, 786, 829, 861
BpmI CTGGAG 3 cut(s) 756, 1221, 2102
Bpu1102I GCTNAGC 1 cut(s) 2928
BpuEI CTTGAG 4 cut(s) 394, 468, 781, 2954
BsaAI YACGTR 2 cut(s) 787, 1352
BsaBI GATNNNNATC 1 cut(s) 1332
BsaI GGTCTC 2 cut(s) 1065, 2336
BsaJI CCNNGG 6 cut(s) 207, 1215, 1596, 2904, 3031, 3032
BsaWI WCCGGW 1 cut(s) 2001
BsaXI ACNNNNNCTCC 4 cut(s) 1791, 1821, 2115, 2145
Bsc4I CCNNNNNNNGG 7 cut(s) 76, 1015, 1216, 1364, 2675, 2743, 2744
Bse118I RCCGGY 1 cut(s) 1505
Bse3DI GCAATG 5 cut(s) 48, 57, 2019, 2087, 2837
Bse8I GATNNNNATC 1 cut(s) 1332
BseBI CCWGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
BseDI CCNNGG 6 cut(s) 207, 1215, 1596, 2904, 3031, 3032
BseGI GGATG 4 cut(s) 2230, 2395, 2575, 2714
BseJI GATNNNNATC 1 cut(s) 1332
BseLI CCNNNNNNNGG 7 cut(s) 76, 1015, 1216, 1364, 2675, 2743, 2744
BseMI GCAATG 5 cut(s) 48, 57, 2019, 2087, 2837
BseRI GAGGAG 4 cut(s) 449, 2420, 2705, 2869
BseSI GKGCMC 1 cut(s) 1889
BsgI GTGCAG 2 cut(s) 221, 1312
Bsh1236I CGCG 1 cut(s) 1643
BshFI GGCC 4 cut(s) 7, 32, 1667, 2903
BshNI GGYRCC 1 cut(s) 3043
BsiHKAI GWGCWC 2 cut(s) 2412, 2992
BsiHKCI CYCGRG 3 cut(s) 2726, 2952, 3071
BsiSI CCGG 2 cut(s) 1506, 2002
BslFI GGGAC 6 cut(s) 921, 1742, 1947, 2432, 2651, 2720
BslI CCNNNNNNNGG 7 cut(s) 76, 1015, 1216, 1364, 2675, 2743, 2744
BsmAI GTCTC 4 cut(s) 1054, 1065, 2336, 2477
BsmFI GGGAC 6 cut(s) 921, 1742, 1947, 2432, 2651, 2720
BsmI GAATGC 3 cut(s) 1211, 1912, 1967
BsnI GGCC 4 cut(s) 7, 32, 1667, 2903
Bso31I GGTCTC 2 cut(s) 1065, 2336
BsoBI CYCGRG 3 cut(s) 2726, 2952, 3071
Bsp1286I GDGCHC 4 cut(s) 1889, 2412, 2929, 2992
Bsp1407I TGTACA 1 cut(s) 2008
Bsp143I GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
Bsp1720I GCTNAGC 1 cut(s) 2928
Bsp19I CCATGG 1 cut(s) 1215
BspACI CCGC 5 cut(s) 439, 500, 1977, 2500, 2923
BspANI GGCC 4 cut(s) 7, 32, 1667, 2903
BspFNI CGCG 1 cut(s) 1643
BspHI TCATGA 1 cut(s) 2437
BspLI GGNNCC 3 cut(s) 937, 2859, 3045
BspMI ACCTGC 3 cut(s) 633, 1017, 2026
BspPI GGATC 3 cut(s) 2320, 2604, 2774
BspQI GCTCTTC 1 cut(s) 1120
BspT107I GGYRCC 1 cut(s) 3043
BspTNI GGTCTC 2 cut(s) 1065, 2336
BsrBI CCGCTC 1 cut(s) 439
BsrDI GCAATG 5 cut(s) 48, 57, 2019, 2087, 2837
BsrFI RCCGGY 1 cut(s) 1505
BsrGI TGTACA 1 cut(s) 2008
BssAI RCCGGY 1 cut(s) 1505
BssECI CCNNGG 6 cut(s) 207, 1215, 1596, 2904, 3031, 3032
BssMI GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
BssNAI GTATAC 1 cut(s) 1355
BssT1I CCWWGG 4 cut(s) 207, 1215, 1596, 2904
Bst1107I GTATAC 1 cut(s) 1355
Bst2UI CCWGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
Bst4CI ACNGT 8 cut(s) 533, 917, 1148, 1733, 1877, 1916, 2266, 2846
Bst6I CTCTTC 4 cut(s) 186, 1120, 1167, 2887
BstAPI GCANNNNNTGC 2 cut(s) 1290, 2711
BstAUI TGTACA 1 cut(s) 2008
BstBAI YACGTR 2 cut(s) 787, 1352
BstC8I GCNNGC 3 cut(s) 1927, 2789, 2925
BstDSI CCRYGG 1 cut(s) 1215
BstF5I GGATG 4 cut(s) 2230, 2395, 2575, 2714
BstFNI CGCG 1 cut(s) 1643
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 4 cut(s) 85, 221, 1645, 2752
BstKTI GATC 9 cut(s) 1138, 1279, 1330, 1457, 2328, 2353, 2584, 2599, 2782
BstMAI GTCTC 4 cut(s) 1054, 1065, 2336, 2477
BstMBI GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
BstNI CCWGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
BstNSI RCATGY 2 cut(s) 1929, 2791
BstPAI GACNNNNGTC 1 cut(s) 1232
BstSCI CCNGG 7 cut(s) 320, 707, 772, 1795, 2717, 2828, 3031
BstSFI CTRYAG 2 cut(s) 2262, 2673
BstSLI GKGCMC 1 cut(s) 1889
BstSNI TACGTA 1 cut(s) 1352
BstUI CGCG 1 cut(s) 1643
BstX2I RGATCY 2 cut(s) 2325, 2596
BstYI RGATCY 2 cut(s) 2325, 2596
BstZ17I GTATAC 1 cut(s) 1355
BsuI GTATCC 1 cut(s) 1165
BsuRI GGCC 4 cut(s) 7, 32, 1667, 2903
BtgI CCRYGG 1 cut(s) 1215
BtgZI GCGATG 1 cut(s) 1624
BtsCI GGATG 4 cut(s) 2230, 2395, 2575, 2714
BtsI GCAGTG 2 cut(s) 1300, 3009
BtsIMutI CAGTG 8 cut(s) 1020, 1095, 1236, 1300, 2078, 2612, 2842, 3009
BveI ACCTGC 3 cut(s) 633, 1017, 2026
Cac8I GCNNGC 3 cut(s) 1927, 2789, 2925
CaiI CAGNNNCTG 3 cut(s) 587, 1544, 1992
CciI TCATGA 1 cut(s) 2437
CfoI GCGC 4 cut(s) 85, 221, 1645, 2752
Cfr10I RCCGGY 1 cut(s) 1505
Cfr13I GGNCC 3 cut(s) 515, 935, 2962
CseI GACGC 1 cut(s) 287
Csp6I GTAC 9 cut(s) 167, 351, 622, 1006, 2009, 2195, 2610, 2696, 3044
CviAII CATG 8 cut(s) 1216, 1926, 2150, 2366, 2438, 2711, 2784, 2788
CviQI GTAC 9 cut(s) 167, 351, 622, 1006, 2009, 2195, 2610, 2696, 3044
DpnI GATC 9 cut(s) 1137, 1278, 1329, 1456, 2327, 2352, 2583, 2598, 2781
DpnII GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
DraI TTTAAA 1 cut(s) 2850
DrdI GACNNNNNNGTC 1 cut(s) 2264
DseDI GACNNNNNNGTC 1 cut(s) 2264
EaeI YGGCCR 2 cut(s) 5, 2901
Eam1104I CTCTTC 4 cut(s) 186, 1120, 1167, 2887
EarI CTCTTC 4 cut(s) 186, 1120, 1167, 2887
Ecl136II GAGCTC 1 cut(s) 2410
Eco105I TACGTA 1 cut(s) 1352
Eco130I CCWWGG 4 cut(s) 207, 1215, 1596, 2904
Eco147I AGGCCT 2 cut(s) 32, 1667
Eco24I GRGCYC 2 cut(s) 2412, 2929
Eco31I GGTCTC 2 cut(s) 1065, 2336
Eco47I GGWCC 3 cut(s) 515, 935, 2962
Eco53kI GAGCTC 1 cut(s) 2410
Eco57I CTGAAG 6 cut(s) 84, 107, 306, 693, 2073, 2780
Eco72I CACGTG 1 cut(s) 787
Eco88I CYCGRG 3 cut(s) 2726, 2952, 3071
EcoICRI GAGCTC 1 cut(s) 2410
EcoO109I RGGNCCY 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 7 cut(s) 320, 707, 772, 1795, 2717, 2828, 3031
EcoT14I CCWWGG 4 cut(s) 207, 1215, 1596, 2904
EcoT22I ATGCAT 4 cut(s) 402, 1184, 2716, 2789
EcoT38I GRGCYC 2 cut(s) 2412, 2929
ErhI CCWWGG 4 cut(s) 207, 1215, 1596, 2904
FaeI CATG 8 cut(s) 1219, 1929, 2153, 2369, 2441, 2714, 2787, 2791
FalI AAGNNNNNCTT 4 cut(s) 1580, 1612, 2086, 2118
FaqI GGGAC 6 cut(s) 921, 1742, 1947, 2432, 2651, 2720
FatI CATG 8 cut(s) 1215, 1925, 2149, 2365, 2437, 2710, 2783, 2787
FauI CCCGC 1 cut(s) 2916
FauNDI CATATG 3 cut(s) 274, 661, 2527
FbaI TGATCA 2 cut(s) 1327, 2581
FblI GTMKAC 3 cut(s) 1354, 2268, 2672
FokI GGATG 4 cut(s) 2237, 2402, 2582, 2701
FriOI GRGCYC 2 cut(s) 2412, 2929
FspBI CTAG 6 cut(s) 639, 885, 1110, 1892, 2201, 2817
GlaI GCGC 4 cut(s) 84, 220, 1644, 2751
GsuI CTGGAG 3 cut(s) 756, 1221, 2102
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 4 cut(s) 7, 32, 1667, 2903
HapII CCGG 2 cut(s) 1506, 2002
HgaI GACGC 1 cut(s) 287
HhaI GCGC 4 cut(s) 85, 221, 1645, 2752
Hin1II CATG 8 cut(s) 1219, 1929, 2153, 2369, 2441, 2714, 2787, 2791
Hin6I GCGC 4 cut(s) 83, 219, 1643, 2750
HinP1I GCGC 4 cut(s) 83, 219, 1643, 2750
HincII GTYRAC 1 cut(s) 2803
HindII GTYRAC 1 cut(s) 2803
HindIII AAGCTT 3 cut(s) 370, 757, 3051
HpaI GTTAAC 1 cut(s) 2803
HpaII CCGG 2 cut(s) 1506, 2002
HphI GGTGA 5 cut(s) 617, 1012, 1160, 1586, 1790
Hpy166II GTNNAC 6 cut(s) 1355, 2219, 2269, 2612, 2673, 2803
Hpy8I GTNNAC 6 cut(s) 1355, 2219, 2269, 2612, 2673, 2803
Hpy99I CGWCG 3 cut(s) 2144, 2538, 2541
HpyAV CCTTC 9 cut(s) 563, 738, 947, 1379, 2048, 2817, 2827, 2938, 2953
HpyCH4III ACNGT 8 cut(s) 533, 917, 1148, 1733, 1877, 1916, 2266, 2846
HpyCH4IV ACGT 2 cut(s) 786, 1351
HpySE526I ACGT 2 cut(s) 786, 1351
Hsp92II CATG 8 cut(s) 1219, 1929, 2153, 2369, 2441, 2714, 2787, 2791
HspAI GCGC 4 cut(s) 83, 219, 1643, 2750
KpnI GGTACC 1 cut(s) 3047
Ksp22I TGATCA 2 cut(s) 1327, 2581
KspAI GTTAAC 1 cut(s) 2803
Kzo9I GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
LguI GCTCTTC 1 cut(s) 1120
LmnI GCTCC 7 cut(s) 271, 436, 1367, 1989, 2407, 2857, 2882
LweI GCATC 8 cut(s) 216, 1085, 2194, 2299, 2640, 2723, 2997, 3054
MaeI CTAG 6 cut(s) 639, 885, 1110, 1892, 2201, 2817
MaeII ACGT 2 cut(s) 786, 1351
MalI GATC 9 cut(s) 1137, 1278, 1329, 1456, 2327, 2352, 2583, 2598, 2781
MbiI CCGCTC 1 cut(s) 439
MboI GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
MflI RGATCY 2 cut(s) 2325, 2596
MhlI GDGCHC 4 cut(s) 1889, 2412, 2929, 2992
MlsI TGGCCA 1 cut(s) 2903
MluNI TGGCCA 1 cut(s) 2903
MlyI GAGTC 2 cut(s) 815, 1550
MmeI TCCRAC 3 cut(s) 326, 2101, 2557
Mox20I TGGCCA 1 cut(s) 2903
Mph1103I ATGCAT 4 cut(s) 402, 1184, 2716, 2789
MroXI GAANNNNTTC 1 cut(s) 2462
MscI TGGCCA 1 cut(s) 2903
MslI CAYNNNNRTG 2 cut(s) 1489, 2024
Msp20I TGGCCA 1 cut(s) 2903
MspI CCGG 2 cut(s) 1506, 2002
MspR9I CCNGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
Mva1269I GAATGC 3 cut(s) 1211, 1912, 1967
MvaI CCWGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
MvnI CGCG 1 cut(s) 1643
NcoI CCATGG 1 cut(s) 1215
NdeI CATATG 3 cut(s) 274, 661, 2527
NdeII GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
NlaIII CATG 8 cut(s) 1219, 1929, 2153, 2369, 2441, 2714, 2787, 2791
NlaIV GGNNCC 3 cut(s) 937, 2859, 3045
NmuCI GTSAC 5 cut(s) 880, 1225, 1234, 2354, 2495
NsiI ATGCAT 4 cut(s) 402, 1184, 2716, 2789
NspI RCATGY 2 cut(s) 1929, 2791
PaeI GCATGC 2 cut(s) 1929, 2791
PaeR7I CTCGAG 3 cut(s) 2726, 2952, 3071
PagI TCATGA 1 cut(s) 2437
PasI CCCWGGG 1 cut(s) 3032
PceI AGGCCT 2 cut(s) 32, 1667
PciSI GCTCTTC 1 cut(s) 1120
PctI GAATGC 3 cut(s) 1211, 1912, 1967
PdmI GAANNNNTTC 1 cut(s) 2462
PflMI CCANNNNNTGG 1 cut(s) 1216
PfoI TCCNGGA 1 cut(s) 772
PleI GAGTC 2 cut(s) 815, 1549
PmaCI CACGTG 1 cut(s) 787
PmlI CACGTG 1 cut(s) 787
PpsI GAGTC 2 cut(s) 815, 1549
Ppu21I YACGTR 2 cut(s) 787, 1352
PpuMI RGGWCCY 1 cut(s) 935
PshAI GACNNNNGTC 1 cut(s) 1232
PshBI ATTAAT 1 cut(s) 1256
PsiI TTATAA 2 cut(s) 1955, 1971
Psp124BI GAGCTC 1 cut(s) 2412
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 7 cut(s) 320, 707, 772, 1795, 2717, 2828, 3031
PspCI CACGTG 1 cut(s) 787
PspGI CCWGG 7 cut(s) 320, 707, 772, 1795, 2717, 2828, 3031
PspN4I GGNNCC 3 cut(s) 937, 2859, 3045
PspPI GGNCC 3 cut(s) 515, 935, 2962
PspPPI RGGWCCY 1 cut(s) 935
PspXI VCTCGAGB 1 cut(s) 3071
PstNI CAGNNNCTG 3 cut(s) 587, 1544, 1992
PsuI RGATCY 2 cut(s) 2325, 2596
RsaI GTAC 9 cut(s) 168, 352, 623, 1007, 2010, 2196, 2611, 2697, 3045
RsaNI GTAC 9 cut(s) 167, 351, 622, 1006, 2009, 2195, 2610, 2696, 3044
RseI CAYNNNNRTG 2 cut(s) 1489, 2024
SacI GAGCTC 1 cut(s) 2412
SapI GCTCTTC 1 cut(s) 1120
Sau3AI GATC 9 cut(s) 1135, 1276, 1327, 1454, 2325, 2350, 2581, 2596, 2779
Sau96I GGNCC 3 cut(s) 515, 935, 2962
ScaI AGTACT 1 cut(s) 2196
SchI GAGTC 2 cut(s) 815, 1550
ScrFI CCNGG 7 cut(s) 322, 709, 774, 1797, 2719, 2830, 3033
SduI GDGCHC 4 cut(s) 1889, 2412, 2929, 2992
SfaNI GCATC 8 cut(s) 216, 1085, 2194, 2299, 2640, 2723, 2997, 3054
SfcI CTRYAG 2 cut(s) 2262, 2673
Sfr274I CTCGAG 3 cut(s) 2726, 2952, 3071
SinI GGWCC 3 cut(s) 515, 935, 2962
SlaI CTCGAG 3 cut(s) 2726, 2952, 3071
SmiMI CAYNNNNRTG 2 cut(s) 1489, 2024
SmlI CTYRAG 7 cut(s) 373, 483, 760, 2726, 2933, 2952, 3071
SmoI CTYRAG 7 cut(s) 373, 483, 760, 2726, 2933, 2952, 3071
SnaBI TACGTA 1 cut(s) 1352
SpeI ACTAGT 1 cut(s) 1891
SphI GCATGC 2 cut(s) 1929, 2791
SseBI AGGCCT 2 cut(s) 32, 1667
SsiI CCGC 5 cut(s) 439, 500, 1977, 2500, 2923
SspMI CTAG 6 cut(s) 639, 885, 1110, 1892, 2201, 2817
SstI GAGCTC 1 cut(s) 2412
StuI AGGCCT 2 cut(s) 32, 1667
StyD4I CCNGG 7 cut(s) 320, 707, 772, 1795, 2717, 2828, 3031
StyI CCWWGG 4 cut(s) 207, 1215, 1596, 2904
TaaI ACNGT 8 cut(s) 533, 917, 1148, 1733, 1877, 1916, 2266, 2846
TaiI ACGT 2 cut(s) 789, 1354
TaqI TCGA 7 cut(s) 463, 847, 2139, 2322, 2727, 2953, 3072
TatI WGTACW 2 cut(s) 2008, 2194
TauI GCSGC 1 cut(s) 442
TscAI CASTG 8 cut(s) 1020, 1095, 1243, 1300, 2085, 2619, 2849, 3009
TseFI GTSAC 5 cut(s) 880, 1225, 1234, 2354, 2495
Tsp45I GTSAC 5 cut(s) 880, 1225, 1234, 2354, 2495
TspGWI ACGGA 2 cut(s) 1960, 2286
TspRI CASTG 8 cut(s) 1020, 1095, 1243, 1300, 2085, 2619, 2849, 3009
Van91I CCANNNNNTGG 1 cut(s) 1216
VpaK11BI GGWCC 3 cut(s) 515, 935, 2962
VspI ATTAAT 1 cut(s) 1256
XapI RAATTY 4 cut(s) 50, 247, 967, 1648
XceI RCATGY 2 cut(s) 1929, 2791
XcmI CCANNNNNNNNNTGG 2 cut(s) 873, 2896
XhoI CTCGAG 3 cut(s) 2726, 2952, 3071
XmiI GTMKAC 3 cut(s) 1354, 2268, 2672
XmnI GAANNNNTTC 1 cut(s) 2462
XspI CTAG 6 cut(s) 639, 885, 1110, 1892, 2201, 2817
ZrmI AGTACT 1 cut(s) 2196
Zsp2I ATGCAT 4 cut(s) 402, 1184, 2716, 2789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.