pycom02g22720

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000439
Physical Location & Seq
Reverse (-)
20909890 .. 20913650
3761 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22720.2

Sequence Viewer

Length: 2259 bp
ATGACGCCAATGCCGAAGAAAAATCGAAAGATGGCGGCTGATGCCTGCCGTGCAATGAAGGAGTTTGGCATTTCGGAAGTTCGAACGAGGGCGGGGGTTAAGGAACTTCTGAAATTGTATGAGAACAACTGGGAGCTTATTGTGGAGGACAATCACAGAGTGCTGCTTGAGTTTTTATTGGAAGGTGACACTGACAACAACAAGGAAGAGGAAGAGGAAGAGGCAGAGGCAGACGAAGAGATAGTGGAACAAAAGGGCAAAGGAGTTTCGATATGTGAATCAAAGGCTGTGAAACGTAAGAACAAAGCAGCTCCATCTCATGGTAAAACTGAAGATGTTCAACTTCCAATCAAGAGATATTGCACAAGGCAACAGACAAAACAAGCATTGTCCCCTTTGAAATGCGCCACACCTTGCCTTGTAGAAACTCCATCAAAAATGGCACATCAGAATGAGGATTCTGAGGAATCTGAAGACTCTCAACCACTAGTGAGAAGAAGTTGCCGGAGACTCCAAGAAGGAGAGGTTTTGGTTAGTGGGAATAACTTCAGTCCTGTACGTAATCGCAATACACAGCTGGAAGAAAGTCTCAATATGATGTGTGAAGATAGTTTTTCAGATTTTGATGACGGTGGCTTGAAGTTCTTGGAAGTGAAATCATCCACTGTGGAGAGTAGAGGAGCTGTTTTAGATTTGTCGCTGTTTGATGTTGAATCATCTCCTCCAAAGGGAGAGCTCGAAAGGTCTTTGATTTGTCACCCTTCTCTACATTCTGATTTCGATCTTCCACATTCTGATGAAGCTCTAGAATTATTGGAAGAGACATGCATGAAATCGTACAGGGTCAGAGAATGCTTAGTGGAGACAGGATTTGATTCCACTTTTGATGAAAGTACAATTTCCAGAGATGTAGCAGAGTTAAAGAATTCTGATGCACAAGATGTTCTTGAATCCCATTATCAAGGCAATGATTGCAATACTCTAAGTTCCCCGAGCGAATTATTTGTATTTCAGAATCTTATCAAGGTCGTTCCCCATATACCCAAGCATATAGCTTTTGGCTGTTTTAATGATCTTCACCGCTTAATTGGTTTTTCAAGTATGGGTATTGAGAGCATCTGTGGTGAATTGGCAAAGAGATTAGTGATTTTCCAAGGCCATAAATCTCCAAAGTTACGCAAGTTGGAGGAGGTTGCTCATGACTACCATTCTTCCATTGGAGTTATAAAATCCGGTGTTTATATTGAAGACATAACTAGAGGTGAGGAGAGAGTGAAAATTTCCTTGGAGGATGGAAAAAATGTTGAAGATCTGCCAAACTTTCTTTACATCCGTCAGAGTTTAGTTTATAAGAATGCCTCTGTGAAATTTTCACTTGGTCGTATGTCTCATGACAGTTGCTATTCACGTTGTTATGGGGATTGTCTGACATCGCCCATGCCTTGTGTCTGTGCTTCTGAAACTGGGGGTCAGTTTGCTTACACACCAGGAGGATTGGTCAAAGAGAAGTTTCTGGAAAAATGTATAGCCTTGAAGCGGGAACCTATACAACACCACTGCTTCTATTGTAGAGAGTGCCCTCTTGAAAAGTCTAAGTACGAGAAGAGTTCTGTGGCATGCAAAGGCCATTTGTTACATAAATTTATTAAGGAGTGCTGGCATACATGTGGATGCAATAAGAACTGTGGAAACCGTATTGTTCAGCAAGGCATCAGGGGGAAGTTGCAGGTATTTTTGACACCCGAAGGGAAAGGATGGGGTCTTCGAACACTGGAGGACTTGCCAAGAGGAGCTTTTGTTTGTGAGTATGTTGGAGAGATATTAACCAACACAGAACTACATGAGCGAAATGTGCCAAGTACTGGTAAGAAGCATGCATACTACGCAGTGCCACTAGATGCGGGCTGGGGTATGAAGGGTGTCTTGAAAGATGAAGAGGCACTTTGCTTGGACGCTACTGTTTATGGAAATGTTGCAAGATTTATCAATCACAGGTGTTCTGATGCTACCTTGGTTGAGATTCCTGTTGAAGTGGAGACCCCGGATCGTCACTATTATCATCTTGCCCTTTTCACAACAAGAAACATTGCTGCTATGGAGGAGCTCACTTGGGACTATGGCATTGACTTTTGTGAGTTTGATCATCCCATGAATGCGTTTCAATGTCTCTGTGGGAGCCCACTCTGCCGTGATCGTAAGGAGTCCAAGGGTGACGTGCAGCTACGCACTTACGGTCGACGAAGACGATGCAGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SET WIYLD

Protein Analysis

753

Amino Acids

85.13

Weight (kDa)

5.8

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000439)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380
fragaria_vesca FvH4_2g02250 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_5g31170 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620
malus_domestica MD02G1265700.v1.1 MD04G1231900.v1.1 MD12G1043600.v1.1 MD12G1250000.v1.1
prunus_persica Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1
pyrus_communis pycom02g22720 pycom04g20520
rosa_chinensis RchiOBHm_Chr3g0449441 RchiOBHm_Chr4g0395181 RchiOBHm_Chr6g0245941 RchiOBHm_Chr7g0228041
rosa_laevigata RLG00000001593 RLG00000009641 RLG00000015284 RLG00000025830
rosa_multiflora Rmu_co8250301.1_g000001 Rmu_co8518901.1_g000001 Rmu_sc0004858.1_g000011 Rmu_sc0007224.1_g000005 Rmu_sc0007891.1_g000010 Rmu_sc0019478.1_g000002 Rmu_sc0024829.1_g000001 Rmu_sc0039188.1_g000001
rosa_roxburghii Rroxscaffold_3G00231620 Rroxscaffold_5G00340120 Rroxscaffold_6G00427390 Rroxscaffold_7G00215050
rosa_rugosa Rorug02G0620600 Rorug03G0359100.1 Rorug03G0359200.1 Rorug03G0359300.1 Rorug05G0517600 Rorug05G0517700 Rorug05G0517800 Rorug05G0517900 Rorug05G0518000 Rorug05G0518100 Rorug05G0518200 Rorug05G0518300.1 Rorug07G0245600
rosa_samantha Rh3AG018400 Rh3BG018800 Rh3CG017700 Rh4AG057300 Rh4BG056200 Rh4CG062300 Rh4DG053200 Rh6AG032500 Rh6BG028300 Rh6CG028300 Rh6CG028500 Rh6CG028600 Rh7AG399400 Rh7BG380800 Rh7CG417400 Rh7DG394900
rosa_wichuraiana Rw4G004610 Rw6G002670 Rw7G033190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1226, 1350
Acc36I ACCTGC 1 cut(s) 1717
AccB7I CCANNNNNTGG 2 cut(s) 320, 1862
AccI GTMKAC 1 cut(s) 2236
AciI CCGC 5 cut(s) 35, 92, 1081, 1537, 1901
AclWI GGATC 1 cut(s) 2052
AcsI RAATTY 4 cut(s) 925, 1278, 1367, 1640
AcuI CTGAAG 3 cut(s) 351, 492, 532
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 5 cut(s) 558, 839, 895, 1598, 1861
AfiI CCNNNNNNNGG 4 cut(s) 320, 728, 1536, 1862
AflIII ACRYGT 1 cut(s) 1664
AhlI ACTAGT 1 cut(s) 487
AjiI CACGTC 1 cut(s) 2215
AjnI CCWGG 1 cut(s) 1486
AjuI GAANNNNNNNTTGG 2 cut(s) 1268, 1300
Alw21I GWGCWC 2 cut(s) 738, 2106
Alw26I GTCTC 7 cut(s) 502, 593, 815, 857, 1392, 2030, 2171
AlwI GGATC 1 cut(s) 2052
Ama87I CYCGRG 1 cut(s) 991
AoxI GGCC 2 cut(s) 1156, 1624
ApeKI GCWGC 4 cut(s) 163, 308, 2090, 2218
ApoI RAATTY 4 cut(s) 925, 1278, 1367, 1640
Asp700I GAANNNNTTC 2 cut(s) 336, 545
AspLEI GCGC 1 cut(s) 407
AsuC2I CCSGG 1 cut(s) 2042
AsuHPI GGTGA 6 cut(s) 197, 749, 1070, 1136, 1274, 2222
AsuII TTCGAA 2 cut(s) 82, 1765
AvaI CYCGRG 1 cut(s) 991
BaeGI GKGCMC 1 cut(s) 1580
BanII GRGCYC 3 cut(s) 738, 2106, 2180
BarI GAAGNNNNNNTAC 2 cut(s) 1862, 1894
BbsI GAAGAC 4 cut(s) 480, 1254, 1754, 2248
Bbv12I GWGCWC 2 cut(s) 738, 2106
BbvI GCAGC 4 cut(s) 150, 320, 2077, 2230
BccI CCATC 5 cut(s) 25, 322, 439, 1286, 1749
BceAI ACGGC 2 cut(s) 33, 2172
BcgI CGANNNNNNTGC 1 cut(s) 2229
BciT130I CCWGG 1 cut(s) 1488
BclI TGATCA 1 cut(s) 2140
BcnI CCSGG 1 cut(s) 2042
BcoDI GTCTC 7 cut(s) 502, 593, 815, 857, 1392, 2030, 2171
BcuI ACTAGT 1 cut(s) 487
BfaI CTAG 4 cut(s) 488, 806, 1257, 1895
BfuAI ACCTGC 1 cut(s) 1717
BglII AGATCT 1 cut(s) 1309
BisI GCNGC 5 cut(s) 36, 164, 309, 2091, 2219
BlsI GCNGC 5 cut(s) 37, 165, 310, 2092, 2220
BmcAI AGTACT 1 cut(s) 1861
Bme1390I CCNGG 2 cut(s) 1488, 2042
BmeT110I CYCGRG 1 cut(s) 991
BmgBI CACGTC 1 cut(s) 2215
BmiI GGNNCC 2 cut(s) 1542, 2177
BmrFI CCNGG 2 cut(s) 1488, 2042
BmrI ACTGGG 2 cut(s) 139, 1473
BmsI GCATC 8 cut(s) 31, 922, 1125, 1661, 1719, 1888, 1993, 2237
BmuI ACTGGG 2 cut(s) 139, 1473
BpiI GAAGAC 4 cut(s) 480, 1254, 1754, 2248
BpmI CTGGAG 1 cut(s) 1793
Bpu14I TTCGAA 2 cut(s) 82, 1765
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 2042
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 780
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 2030
BsaJI CCNNGG 5 cut(s) 1153, 1284, 2010, 2040, 2205
BsaWI WCCGGW 1 cut(s) 1232
BsaXI ACNNNNNCTCC 4 cut(s) 1482, 1512, 1782, 1812
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 728, 1536, 1862
Bse1I ACTGG 4 cut(s) 134, 1468, 1776, 1867
Bse3DI GCAATG 3 cut(s) 60, 973, 2085
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 1 cut(s) 1488
BseDI CCNNGG 5 cut(s) 1153, 1284, 2010, 2040, 2205
BseGI GGATG 6 cut(s) 659, 1297, 1329, 1676, 1760, 2143
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 4 cut(s) 320, 728, 1536, 1862
BseMI GCAATG 3 cut(s) 60, 973, 2085
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 4 cut(s) 134, 1468, 1776, 1867
BseRI GAGGAG 6 cut(s) 693, 711, 1202, 1280, 1803, 2114
BseSI GKGCMC 1 cut(s) 1580
BseXI GCAGC 4 cut(s) 150, 320, 2077, 2230
BseYI CCCAGC 1 cut(s) 1905
BsgI GTGCAG 1 cut(s) 2237
Bsh1285I CGRYCG 1 cut(s) 2236
BshFI GGCC 2 cut(s) 1158, 1626
BsiEI CGRYCG 1 cut(s) 2236
BsiHKAI GWGCWC 2 cut(s) 738, 2106
BsiHKCI CYCGRG 1 cut(s) 991
BsiSI CCGG 3 cut(s) 505, 1233, 2042
BslFI GGGAC 2 cut(s) 376, 2126
BslI CCNNNNNNNGG 4 cut(s) 320, 728, 1536, 1862
BsmAI GTCTC 7 cut(s) 502, 593, 815, 857, 1392, 2030, 2171
BsmFI GGGAC 2 cut(s) 376, 2126
BsmI GAATGC 3 cut(s) 857, 1360, 2158
BsnI GGCC 2 cut(s) 1158, 1626
Bso31I GGTCTC 1 cut(s) 2030
BsoBI CYCGRG 1 cut(s) 991
Bsp119I TTCGAA 2 cut(s) 82, 1765
Bsp1286I GDGCHC 4 cut(s) 738, 1580, 2106, 2180
Bsp143I GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
BspACI CCGC 5 cut(s) 35, 92, 1081, 1537, 1901
BspANI GGCC 2 cut(s) 1158, 1626
BspCNI CTCAG 1 cut(s) 454
BspHI TCATGA 2 cut(s) 1198, 1390
BspLI GGNNCC 2 cut(s) 1542, 2177
BspMI ACCTGC 1 cut(s) 1717
BspPI GGATC 1 cut(s) 2052
BspT104I TTCGAA 2 cut(s) 82, 1765
BspTNI GGTCTC 1 cut(s) 2030
BsrDI GCAATG 3 cut(s) 60, 973, 2085
BsrI ACTGG 4 cut(s) 134, 1468, 1776, 1867
BssECI CCNNGG 5 cut(s) 1153, 1284, 2010, 2040, 2205
BssMI GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 4 cut(s) 1153, 1284, 2010, 2205
Bst2UI CCWGG 1 cut(s) 1488
Bst4CI ACNGT 7 cut(s) 632, 667, 1397, 1685, 1694, 1960, 2234
Bst6I CTCTTC 7 cut(s) 201, 207, 213, 231, 813, 1598, 1929
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 972
BstBAI YACGTR 1 cut(s) 560
BstBI TTCGAA 2 cut(s) 82, 1765
BstC8I GCNNGC 5 cut(s) 46, 1618, 1658, 1875, 1903
BstDEI CTNAG 4 cut(s) 462, 856, 983, 1593
BstF5I GGATG 6 cut(s) 659, 1297, 1329, 1676, 1760, 2143
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 6 cut(s) 784, 1075, 1312, 2047, 2143, 2194
BstMAI GTCTC 7 cut(s) 502, 593, 815, 857, 1392, 2030, 2171
BstMBI GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
BstMCI CGRYCG 1 cut(s) 2236
BstMWI GCNNNNNNNGC 6 cut(s) 41, 50, 972, 1852, 1883, 2184
BstNI CCWGG 1 cut(s) 1488
BstNSI RCATGY 4 cut(s) 828, 1620, 1668, 1877
BstSCI CCNGG 2 cut(s) 1486, 2040
BstSLI GKGCMC 1 cut(s) 1580
BstSNI TACGTA 1 cut(s) 560
BstV1I GCAGC 4 cut(s) 150, 320, 2077, 2230
BstV2I GAAGAC 4 cut(s) 480, 1254, 1754, 2248
BstX2I RGATCY 1 cut(s) 1309
BstYI RGATCY 1 cut(s) 1309
BsuRI GGCC 2 cut(s) 1158, 1626
BtgZI GCGATG 1 cut(s) 1416
BtrI CACGTC 1 cut(s) 2215
BtsCI GGATG 6 cut(s) 659, 1297, 1329, 1676, 1760, 2143
BtsI GCAGTG 2 cut(s) 1555, 1893
BtsIMutI CAGTG 5 cut(s) 189, 663, 1555, 1769, 1893
BveI ACCTGC 1 cut(s) 1717
Cac8I GCNNGC 5 cut(s) 46, 1618, 1658, 1875, 1903
CciI TCATGA 2 cut(s) 1198, 1390
CfoI GCGC 1 cut(s) 407
CseI GACGC 2 cut(s) 13, 1961
Csp6I GTAC 5 cut(s) 557, 838, 894, 1597, 1860
CviQI GTAC 5 cut(s) 557, 838, 894, 1597, 1860
DdeI CTNAG 4 cut(s) 462, 856, 983, 1593
DpnI GATC 6 cut(s) 783, 1074, 1311, 2046, 2142, 2193
DpnII GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
DraIII CACNNNGTG 1 cut(s) 160
Eam1104I CTCTTC 7 cut(s) 201, 207, 213, 231, 813, 1598, 1929
EarI CTCTTC 7 cut(s) 201, 207, 213, 231, 813, 1598, 1929
Ecl136II GAGCTC 2 cut(s) 736, 2104
Eco105I TACGTA 1 cut(s) 560
Eco130I CCWWGG 4 cut(s) 1153, 1284, 2010, 2205
Eco24I GRGCYC 3 cut(s) 738, 2106, 2180
Eco31I GGTCTC 1 cut(s) 2030
Eco53kI GAGCTC 2 cut(s) 736, 2104
Eco57I CTGAAG 3 cut(s) 351, 492, 532
Eco88I CYCGRG 1 cut(s) 991
EcoICRI GAGCTC 2 cut(s) 736, 2104
EcoRI GAATTC 1 cut(s) 925
EcoRII CCWGG 1 cut(s) 1486
EcoT14I CCWWGG 4 cut(s) 1153, 1284, 2010, 2205
EcoT22I ATGCAT 2 cut(s) 830, 1879
EcoT38I GRGCYC 3 cut(s) 738, 2106, 2180
ErhI CCWWGG 4 cut(s) 1153, 1284, 2010, 2205
FalI AAGNNNNNCTT 8 cut(s) 930, 962, 1777, 1809, 1907, 1939, 1926, 1958
FaqI GGGAC 2 cut(s) 376, 2126
FauI CCCGC 3 cut(s) 85, 1530, 1894
FbaI TGATCA 1 cut(s) 2140
FblI GTMKAC 1 cut(s) 2236
Fnu4HI GCNGC 5 cut(s) 36, 164, 309, 2091, 2219
FokI GGATG 6 cut(s) 646, 1304, 1316, 1683, 1767, 2130
FriOI GRGCYC 3 cut(s) 738, 2106, 2180
Fsp4HI GCNGC 5 cut(s) 36, 164, 309, 2091, 2219
FspBI CTAG 4 cut(s) 488, 806, 1257, 1895
GlaI GCGC 1 cut(s) 406
GluI GCNGC 5 cut(s) 36, 164, 309, 2091, 2219
GsaI CCCAGC 1 cut(s) 1909
GsuI CTGGAG 1 cut(s) 1793
HaeIII GGCC 2 cut(s) 1158, 1626
HapII CCGG 3 cut(s) 505, 1233, 2042
HgaI GACGC 2 cut(s) 13, 1961
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 2237
HindII GTYRAC 1 cut(s) 2237
HpaII CCGG 3 cut(s) 505, 1233, 2042
HphI GGTGA 6 cut(s) 197, 749, 1070, 1136, 1274, 2222
Hpy166II GTNNAC 1 cut(s) 2237
Hpy188III TCNNGA 9 cut(s) 352, 806, 903, 947, 1199, 1391, 1514, 1583, 1924
Hpy8I GTNNAC 1 cut(s) 2237
Hpy99I CGWCG 1 cut(s) 2241
HpyAV CCTTC 6 cut(s) 52, 176, 512, 771, 1739, 1909
HpyCH4III ACNGT 7 cut(s) 632, 667, 1397, 1685, 1694, 1960, 2234
HpyCH4IV ACGT 4 cut(s) 295, 559, 1408, 2214
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 50, 972, 1852, 1883, 2184
HpyF3I CTNAG 4 cut(s) 462, 856, 983, 1593
HpySE526I ACGT 4 cut(s) 295, 559, 1408, 2214
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 405
Ksp22I TGATCA 1 cut(s) 2140
Kzo9I GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
LmnI GCTCC 6 cut(s) 133, 316, 680, 1790, 2101, 2175
Lsp1109I GCAGC 4 cut(s) 150, 320, 2077, 2230
LweI GCATC 8 cut(s) 31, 922, 1125, 1661, 1719, 1888, 1993, 2237
MaeI CTAG 4 cut(s) 488, 806, 1257, 1895
MaeII ACGT 4 cut(s) 295, 559, 1408, 2214
MaeIII GTNAC 6 cut(s) 185, 755, 1173, 1632, 2048, 2210
MalI GATC 6 cut(s) 783, 1074, 1311, 2046, 2142, 2193
MboI GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
MflI RGATCY 1 cut(s) 1309
MhlI GDGCHC 4 cut(s) 738, 1580, 2106, 2180
MlyI GAGTC 3 cut(s) 470, 504, 2210
MmeI TCCRAC 2 cut(s) 1164, 1792
Mph1103I ATGCAT 2 cut(s) 830, 1879
MroXI GAANNNNTTC 2 cut(s) 336, 545
MseI TTAA 6 cut(s) 99, 920, 1068, 1085, 1647, 1823
MslI CAYNNNNRTG 4 cut(s) 450, 795, 1665, 1669
MspA1I CMGCKG 1 cut(s) 577
MspI CCGG 3 cut(s) 505, 1233, 2042
MspR9I CCNGG 2 cut(s) 1488, 2042
Mva1269I GAATGC 3 cut(s) 857, 1360, 2158
MvaI CCWGG 1 cut(s) 1488
MwoI GCNNNNNNNGC 6 cut(s) 41, 50, 972, 1852, 1883, 2184
NciI CCSGG 1 cut(s) 2042
NdeII GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
NlaIV GGNNCC 2 cut(s) 1542, 2177
NmuCI GTSAC 4 cut(s) 185, 755, 2048, 2210
NsiI ATGCAT 2 cut(s) 830, 1879
NspI RCATGY 4 cut(s) 828, 1620, 1668, 1877
NspV TTCGAA 2 cut(s) 82, 1765
PaeI GCATGC 2 cut(s) 1620, 1877
PagI TCATGA 2 cut(s) 1198, 1390
PciI ACATGT 1 cut(s) 1664
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 2242
PctI GAATGC 3 cut(s) 857, 1360, 2158
PdmI GAANNNNTTC 2 cut(s) 336, 545
PfeI GAWTC 8 cut(s) 278, 458, 467, 713, 875, 950, 1015, 2020
PflMI CCANNNNNTGG 2 cut(s) 320, 1862
PkrI GCNGC 5 cut(s) 37, 165, 310, 2092, 2220
PleI GAGTC 3 cut(s) 470, 504, 2209
PpsI GAGTC 3 cut(s) 470, 504, 2209
Ppu21I YACGTR 1 cut(s) 560
PscI ACATGT 1 cut(s) 1664
PsiI TTATAA 2 cut(s) 1226, 1350
Psp124BI GAGCTC 2 cut(s) 738, 2106
Psp6I CCWGG 1 cut(s) 1486
PspFI CCCAGC 1 cut(s) 1905
PspGI CCWGG 1 cut(s) 1486
PspN4I GGNNCC 2 cut(s) 1542, 2177
PsuI RGATCY 1 cut(s) 1309
PvuII CAGCTG 1 cut(s) 577
RsaI GTAC 5 cut(s) 558, 839, 895, 1598, 1861
RsaNI GTAC 5 cut(s) 557, 838, 894, 1597, 1860
RseI CAYNNNNRTG 4 cut(s) 450, 795, 1665, 1669
SacI GAGCTC 2 cut(s) 738, 2106
SalI GTCGAC 1 cut(s) 2235
SaqAI TTAA 6 cut(s) 99, 920, 1068, 1085, 1647, 1823
SatI GCNGC 5 cut(s) 36, 164, 309, 2091, 2219
Sau3AI GATC 6 cut(s) 781, 1072, 1309, 2044, 2140, 2191
ScaI AGTACT 1 cut(s) 1861
SchI GAGTC 3 cut(s) 470, 504, 2210
ScrFI CCNGG 2 cut(s) 1488, 2042
SduI GDGCHC 4 cut(s) 738, 1580, 2106, 2180
SfaNI GCATC 8 cut(s) 31, 922, 1125, 1661, 1719, 1888, 1993, 2237
SfuI TTCGAA 2 cut(s) 82, 1765
SmiMI CAYNNNNRTG 4 cut(s) 450, 795, 1665, 1669
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SnaBI TACGTA 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 487
SphI GCATGC 2 cut(s) 1620, 1877
SsiI CCGC 5 cut(s) 35, 92, 1081, 1537, 1901
SspMI CTAG 4 cut(s) 488, 806, 1257, 1895
SstI GAGCTC 2 cut(s) 738, 2106
StyD4I CCNGG 2 cut(s) 1486, 2040
StyI CCWWGG 4 cut(s) 1153, 1284, 2010, 2205
TaaI ACNGT 7 cut(s) 632, 667, 1397, 1685, 1694, 1960, 2234
TaiI ACGT 4 cut(s) 298, 562, 1411, 2217
TaqI TCGA 7 cut(s) 25, 82, 269, 738, 780, 1765, 2236
TatI WGTACW 2 cut(s) 893, 1859
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 8 cut(s) 278, 458, 467, 713, 875, 950, 1015, 2020
Tru1I TTAA 6 cut(s) 99, 920, 1068, 1085, 1647, 1823
Tru9I TTAA 6 cut(s) 99, 920, 1068, 1085, 1647, 1823
TscAI CASTG 5 cut(s) 196, 670, 1562, 1776, 1893
TseFI GTSAC 4 cut(s) 185, 755, 2048, 2210
TseI GCWGC 4 cut(s) 163, 308, 2090, 2218
Tsp45I GTSAC 4 cut(s) 185, 755, 2048, 2210
TspDTI ATGAA 7 cut(s) 71, 813, 845, 903, 1928, 1947, 2165
TspGWI ACGGA 1 cut(s) 1322
TspRI CASTG 5 cut(s) 196, 670, 1562, 1776, 1893
Van91I CCANNNNNTGG 2 cut(s) 320, 1862
XapI RAATTY 4 cut(s) 925, 1278, 1367, 1640
XbaI TCTAGA 1 cut(s) 805
XceI RCATGY 4 cut(s) 828, 1620, 1668, 1877
XcmI CCANNNNNNNNNTGG 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 2236
XmnI GAANNNNTTC 2 cut(s) 336, 545
XspI CTAG 4 cut(s) 488, 806, 1257, 1895
ZrmI AGTACT 1 cut(s) 1861
Zsp2I ATGCAT 2 cut(s) 830, 1879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.