Rh4DG053200

Histone-lysine n-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000439
Physical Location & Seq
Reverse (-)
8316207 .. 8323588
7382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG053200.1

Sequence Viewer

Length: 2784 bp
ATGAAGCCAAGAGATCGCAAAATTATAAATGCCTGTAGTGCAATGAAGAGATTTGGCATTACACCTGCCAAAACAAAGACAGCACTTAAGACACTTCTGAAATTGTATGAAAATAACTGGGAGCTTATCGAAGATGAGAATTACAAAGTACTTTTTGATGTTTTGATGCCAGAGGAACAGAGAGTGGAACCCAAGGGAAAAGAAACTTTGATTCTTGAATCGGAGGGAATGACAACTTCTTACAAAAAAGTTCCATCAAGTGTCAAAACTGAAGTTTCTGAACTTCCAATCAAGAGATTATTCACAGGACAAGAGGAAAAACATGCATCGTCACTCTCCAAATCTTCCTTGGATTCAAATCCATCAAGAAGAGCACATCAGAATGATGGTGATTCTGGTGCATCTCAGCCATTACTGAGAAGAGCCCGCCTAATTAGATGCCGAGAAGAAGCTTTCATCCGTGAATCAAAGCGGGATATGAAATCAAAGAACAAAGAAGCTCCACCAGATAATAATACTGAAGTTTTTGAACTTCCAACTAAGAGATTATACACAAGACAACAGAAAAAACAGGCATCGTCCCTATCCAAATCATGCATGGAAATACCTCCATCAAGAAGGGCACATCAGAGTGATGATGATCTGTTTACAGATTCTGAAGAAGCTCAACCACTATTGAAAAGAACCCGCCTAAGAGACCAGGAAGGAGAAGCTATGATCTGCGACTCAAAGGGAGTGATTTATAATAACATCAAAAGTCCATCAAATGGTAAAACTGAAGTTGTTGAAGTTCCAACCAATAGATACACAAGACAACGAAAAAAGAAGGCATCCTCTCTCCCCAAATCTTGCGTGGAAACAAGTCTCTCAACAATGGCACATCAGAATGATGGTGGTCTGTTTTATGATTCTGAAGAATCTGAAGAAACTCCACTAATATCTAGAAGAAATCGGGGGAGACACCAGAAAAAGAAAGTTTTGGTTTCTGGGAACAAATTGGTTTCTGGCATTCCACCACCAGCCAAAAGTCCCATTGTGATATGTCTAGATAGTTCTTCAGATAGTGATGTTTGTAATGTTGATGAAGAAAGATTAGAGGCACTGGATGAGGAACCATTGACTGTGGACAACATAGCTTGTGTTTCAGATATGTCACACCACCAGCCGAAGGACGTTTTGGTTTCTGGGAATAAATTGGATTCTGGCATTCCACCACCAGCCAAAAGTCCCATTGTGATATGTCTAGAGAATTCTGCAGATAGTGATGTTTGTAATGTTGATAAAGAAAGATTGGAGGCACTGGATGTGGAACCATTGACTGTGGACAACATAGCTTGTGTTTCAGATATGTCACAGATGGATGATGATGAAGACAGGTTGGAGTTACTGAAAGTAGAACCATTGATTGTGGACAGCAGAGGTTGTGTTTTAGATTCGTCACAGGTTGATGTTGCATCATCTCTTTCAAAGGGAGAACTGAATATTTCTCTGACTTGTAACTCGTTTCCACAGTCTGATATTCATTTTCCATGTATGGAAGTGGTGGAAGAGAGAGGCATCAAATCATATTGCAATCAAGAATGCTCTTTGGCTATAGGAACTGATTCAATTACTGATGAAAATGCAAGGTTCAGAGAGGTGCCCCAGCTACAAAACTCGAAGTCACTAGATGTTCTTGCCATAAAAGATGATTATCAAGGCAGTAATTGCAGTGCTCTAGGATTTCCTACTGAAGTATTTGTATTTCAGAATCTCATCAAGATTGTACCCCATATACCCAGACACATAGATCCTTGTAGATTTGGATGTCTTCACAGTTTAATCGGTTTTCCAATTAAGGATATTGAGAATTTTTTTGGTAAAAGTGGAAAAAGATTAAGGGTTCTCCCAGGCCTGCAATTCTCTAAGTTACGTAAGGTGGAGGCTACTAAGAATTCAGAAGTTGTAAAGTTATGTGTTTATGTAGATAACATAACTAGAGGTGAAGAAAATTTGAAAATTCCCTTGGAAGGTGGAAGAAATGCTGAAGATCTGTCGACATTCTTTTACATGCCTCAGAATGTAGTTTATAAAAATGCCCATGTGAATTTTGCACTTCGTTGTACATCTAACCAGGCTTGGTGTTCACATTGTTTCGGGAATTGTCTGACATCACCGGTACCATGTGCCTGTGCTATGCAAACTGCTGGTGGGTTTGCATACGCACCAGGTGGATCGGTCAAGGATACTTTTATGGAAGAATGTTTGTCAGTGATCCAGGAACGTAAACAACACCACTACTTCTATTGTAAAAAGTGCCCTCTTGAAAGTTTTAGGAAGAAAAAGAGTTCTAGCCCATGCAAAGGCCATTTATTACAGAAATTTGTAAAGGAATGTTGGTCTAAATGTGGATGCAACAAGAACTGTGGGAACCGGATTATTCAACAAGGCATTTCTGTCAAGCTGCAGGTGTTTTTGACACCCGAAGGGAAAGGGTGGGGTCTTCGAACATTGGAGGACTTGCCGAGAGGGGCTTTTGTTTGTGAGTATGTTGGAGAGATAGTAACAATCACAGAACTATATGAGCGAAATATGCAAAGTGCTGGTAAGAGGCATACATACACGGTGCTACTAGATGCAAACTGGCATTCAAAGGGTATCTTGAAAGATGAAGAGGCACTTTGCTTAGACGCAACAGTTTATGGAAATGTTGCAAGGTTTATCAACCACAGGTGTAAAATTCATAATCTCATATTTCTCCTTCCCCTTCTCCACCAGAGGCTCTTGTTTAGTAGTAACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
Pre-SET SET WIYLD

Protein Analysis

927

Amino Acids

104.11

Weight (kDa)

7.59

Isoelectric Point (pI)

55.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WIYLD PF10440 4 - 59 6e-17 Ubiquitin-binding WIYLD domain
Pre-SET PF05033 660 - 805 2.6e-06 Pre-SET motif
SET PF00856 825 - 906 8.8e-11 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000439)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380
fragaria_vesca FvH4_2g02250 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_5g31170 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620
malus_domestica MD02G1265700.v1.1 MD04G1231900.v1.1 MD12G1043600.v1.1 MD12G1250000.v1.1
prunus_persica Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1
pyrus_communis pycom02g22720 pycom04g20520
rosa_chinensis RchiOBHm_Chr3g0449441 RchiOBHm_Chr4g0395181 RchiOBHm_Chr6g0245941 RchiOBHm_Chr7g0228041
rosa_laevigata RLG00000001593 RLG00000009641 RLG00000015284 RLG00000025830
rosa_multiflora Rmu_co8250301.1_g000001 Rmu_co8518901.1_g000001 Rmu_sc0004858.1_g000011 Rmu_sc0007224.1_g000005 Rmu_sc0007891.1_g000010 Rmu_sc0019478.1_g000002 Rmu_sc0024829.1_g000001 Rmu_sc0039188.1_g000001
rosa_roxburghii Rroxscaffold_3G00231620 Rroxscaffold_5G00340120 Rroxscaffold_6G00427390 Rroxscaffold_7G00215050
rosa_rugosa Rorug02G0620600 Rorug03G0359100.1 Rorug03G0359200.1 Rorug03G0359300.1 Rorug05G0517600 Rorug05G0517700 Rorug05G0517800 Rorug05G0517900 Rorug05G0518000 Rorug05G0518100 Rorug05G0518200 Rorug05G0518300.1 Rorug07G0245600
rosa_samantha Rh3AG018400 Rh3BG018800 Rh3CG017700 Rh4AG057300 Rh4BG056200 Rh4CG062300 Rh4DG053200 Rh6AG032500 Rh6BG028300 Rh6CG028300 Rh6CG028500 Rh6CG028600 Rh7AG399400 Rh7BG380800 Rh7CG417400 Rh7DG394900
rosa_wichuraiana Rw4G004610 Rw6G002670 Rw7G033190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 26, 744, 2070
AarI CACCTGC 2 cut(s) 73, 2437
Acc36I ACCTGC 2 cut(s) 73, 2437
Acc65I GGTACC 1 cut(s) 2158
AccB1I GGYRCC 2 cut(s) 1639, 2158
AccB7I CCANNNNNTGG 1 cut(s) 767
AccI GTMKAC 1 cut(s) 2036
AciI CCGC 3 cut(s) 427, 472, 688
AclWI GGATC 3 cut(s) 1784, 2221, 2248
AcsI RAATTY 8 cut(s) 1249, 1849, 1933, 1990, 1998, 2086, 2360, 2718
AcuI CTGAAG 9 cut(s) 291, 540, 678, 798, 933, 942, 1041, 1752, 2046
AdeI CACNNNGTG 1 cut(s) 2210
AfaI GTAC 4 cut(s) 150, 1767, 2104, 2160
AfiI CCNNNNNNNGG 4 cut(s) 767, 1168, 2009, 2342
AflII CTTAAG 1 cut(s) 86
AgeI ACCGGT 1 cut(s) 2155
AjnI CCWGG 5 cut(s) 699, 1888, 2112, 2206, 2256
AjuI GAANNNNNNNTTGG 4 cut(s) 1160, 1192, 1988, 2020
AluBI AGCT 9 cut(s) 124, 452, 500, 665, 713, 1136, 1334, 1648, 2443
AluI AGCT 9 cut(s) 124, 452, 500, 665, 713, 1136, 1334, 1648, 2443
Alw21I GWGCWC 2 cut(s) 376, 1717
Alw26I GTCTC 3 cut(s) 690, 869, 952
AlwI GGATC 3 cut(s) 1784, 2221, 2248
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 2 cut(s) 1891, 2344
ApeKI GCWGC 1 cut(s) 2443
ApoI RAATTY 8 cut(s) 1249, 1849, 1933, 1990, 1998, 2086, 2360, 2718
AsiGI ACCGGT 1 cut(s) 2155
Asp700I GAANNNNTTC 1 cut(s) 1603
Asp718I GGTACC 1 cut(s) 2158
AsuHPI GGTGA 3 cut(s) 401, 1994, 2145
AsuII TTCGAA 1 cut(s) 2485
BaeGI GKGCMC 3 cut(s) 625, 1644, 2300
BanI GGYRCC 2 cut(s) 1639, 2158
BanII GRGCYC 1 cut(s) 427
BbsI GAAGAC 3 cut(s) 1377, 1802, 2474
Bbv12I GWGCWC 2 cut(s) 376, 1717
BbvI GCAGC 1 cut(s) 2430
BccI CCATC 7 cut(s) 262, 370, 380, 619, 769, 884, 1351
BciT130I CCWGG 5 cut(s) 701, 1890, 2114, 2208, 2258
BciVI GTATCC 1 cut(s) 2218
BcoDI GTCTC 3 cut(s) 690, 869, 952
BfaI CTAG 9 cut(s) 942, 1046, 1244, 1667, 1718, 1977, 2331, 2612, 2782
BfmI CTRYAG 4 cut(s) 34, 1254, 1593, 2444
BfrI CTTAAG 1 cut(s) 86
BfuAI ACCTGC 2 cut(s) 73, 2437
BfuI GTATCC 1 cut(s) 2218
BglII AGATCT 1 cut(s) 2029
BisI GCNGC 1 cut(s) 2444
BlsI GCNGC 1 cut(s) 2445
BmcAI AGTACT 1 cut(s) 150
Bme1390I CCNGG 5 cut(s) 701, 1890, 2114, 2208, 2258
BmiI GGNNCC 6 cut(s) 189, 1113, 1311, 1641, 2160, 2411
BmrFI CCNGG 5 cut(s) 701, 1890, 2114, 2208, 2258
BmrI ACTGGG 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 3 cut(s) 1377, 1802, 2474
Bpu14I TTCGAA 1 cut(s) 2485
BsaAI YACGTR 1 cut(s) 1913
BsaBI GATNNNNATC 3 cut(s) 357, 639, 1692
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 4 cut(s) 192, 348, 1888, 2004
BsaWI WCCGGW 2 cut(s) 2155, 2412
BsaXI ACNNNNNCTCC 2 cut(s) 2526, 2556
Bsc4I CCNNNNNNNGG 4 cut(s) 767, 1168, 2009, 2342
Bse118I RCCGGY 1 cut(s) 2155
Bse1I ACTGG 4 cut(s) 122, 1107, 1305, 2627
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 3 cut(s) 357, 639, 1692
BseBI CCWGG 5 cut(s) 701, 1890, 2114, 2208, 2258
BseDI CCNNGG 4 cut(s) 192, 348, 1888, 2004
BseGI GGATG 7 cut(s) 456, 830, 1111, 1309, 1366, 1811, 2396
BseJI GATNNNNATC 3 cut(s) 357, 639, 1692
BseLI CCNNNNNNNGG 4 cut(s) 767, 1168, 2009, 2342
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 407, 419, 2069
BseNI ACTGG 4 cut(s) 122, 1107, 1305, 2627
BseSI GKGCMC 3 cut(s) 625, 1644, 2300
BseXI GCAGC 1 cut(s) 2430
BseYI CCCAGC 1 cut(s) 1644
BshFI GGCC 2 cut(s) 1893, 2346
BshNI GGYRCC 2 cut(s) 1639, 2158
BshTI ACCGGT 1 cut(s) 2155
BsiHKAI GWGCWC 2 cut(s) 376, 1717
BsiSI CCGG 2 cut(s) 2156, 2413
BslFI GGGAC 3 cut(s) 565, 1014, 1212
BslI CCNNNNNNNGG 4 cut(s) 767, 1168, 2009, 2342
BsmAI GTCTC 3 cut(s) 690, 869, 952
BsmFI GGGAC 3 cut(s) 565, 1014, 1212
BsmI GAATGC 4 cut(s) 1008, 1206, 1586, 2626
BsnI GGCC 2 cut(s) 1893, 2346
Bso31I GGTCTC 1 cut(s) 690
Bsp119I TTCGAA 1 cut(s) 2485
Bsp1286I GDGCHC 6 cut(s) 376, 427, 625, 1644, 1717, 2300
Bsp1407I TGTACA 1 cut(s) 2102
Bsp143I GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
BspACI CCGC 3 cut(s) 427, 472, 688
BspANI GGCC 2 cut(s) 1893, 2346
BspCNI CTCAG 3 cut(s) 408, 418, 2068
BspLI GGNNCC 6 cut(s) 189, 1113, 1311, 1641, 2160, 2411
BspMAI CTGCAG 2 cut(s) 1258, 2448
BspMI ACCTGC 2 cut(s) 73, 2437
BspPI GGATC 3 cut(s) 1784, 2221, 2248
BspQI GCTCTTC 2 cut(s) 364, 415
BspT104I TTCGAA 1 cut(s) 2485
BspT107I GGYRCC 2 cut(s) 1639, 2158
BspTI CTTAAG 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 2155
BsrGI TGTACA 1 cut(s) 2102
BsrI ACTGG 4 cut(s) 122, 1107, 1305, 2627
BssAI RCCGGY 1 cut(s) 2155
BssECI CCNNGG 4 cut(s) 192, 348, 1888, 2004
BssMI GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
BssT1I CCWWGG 3 cut(s) 192, 348, 2004
Bst2UI CCWGG 5 cut(s) 701, 1890, 2114, 2208, 2258
Bst4CI ACNGT 7 cut(s) 1123, 1321, 1512, 1817, 2405, 2605, 2677
Bst6I CTCTTC 5 cut(s) 41, 364, 415, 1542, 2646
BstAFI CTTAAG 1 cut(s) 86
BstAPI GCANNNNNTGC 1 cut(s) 1707
BstAUI TGTACA 1 cut(s) 2102
BstBAI YACGTR 1 cut(s) 1913
BstBI TTCGAA 1 cut(s) 2485
BstC8I GCNNGC 2 cut(s) 427, 1895
BstDEI CTNAG 8 cut(s) 405, 416, 540, 692, 1905, 1929, 2055, 2665
BstF5I GGATG 7 cut(s) 456, 830, 1111, 1309, 1366, 1811, 2396
BstKTI GATC 7 cut(s) 16, 643, 720, 1792, 2032, 2216, 2256
BstMAI GTCTC 3 cut(s) 690, 869, 952
BstMBI GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
BstMWI GCNNNNNNNGC 3 cut(s) 38, 1707, 2572
BstNI CCWGG 5 cut(s) 701, 1890, 2114, 2208, 2258
BstNSI RCATGY 2 cut(s) 326, 2053
BstSCI CCNGG 5 cut(s) 699, 1888, 2112, 2206, 2256
BstSFI CTRYAG 4 cut(s) 34, 1254, 1593, 2444
BstSLI GKGCMC 3 cut(s) 625, 1644, 2300
BstSNI TACGTA 1 cut(s) 1913
BstV1I GCAGC 1 cut(s) 2430
BstV2I GAAGAC 3 cut(s) 1377, 1802, 2474
BstX2I RGATCY 2 cut(s) 1789, 2029
BstYI RGATCY 2 cut(s) 1789, 2029
BsuI GTATCC 1 cut(s) 2218
BsuRI GGCC 2 cut(s) 1893, 2346
BtsCI GGATG 7 cut(s) 456, 830, 1111, 1309, 1366, 1811, 2396
BtsI GCAGTG 1 cut(s) 1717
BtsIMutI CAGTG 4 cut(s) 1100, 1298, 1717, 2256
BveI ACCTGC 2 cut(s) 73, 2437
Cac8I GCNNGC 2 cut(s) 427, 1895
CaiI CAGNNNCTG 1 cut(s) 656
Cfr10I RCCGGY 1 cut(s) 2155
CseI GACGC 1 cut(s) 2678
CsiI ACCWGGT 1 cut(s) 2206
Csp6I GTAC 4 cut(s) 149, 1766, 2103, 2159
CspAI ACCGGT 1 cut(s) 2155
CspCI CAANNNNNGTGG 2 cut(s) 2386, 2421
CviAII CATG 8 cut(s) 323, 594, 598, 1530, 2050, 2081, 2163, 2337
CviQI GTAC 4 cut(s) 149, 1766, 2103, 2159
DdeI CTNAG 8 cut(s) 405, 416, 540, 692, 1905, 1929, 2055, 2665
DpnI GATC 7 cut(s) 15, 642, 719, 1791, 2031, 2215, 2255
DpnII GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
DraIII CACNNNGTG 1 cut(s) 2210
Eam1104I CTCTTC 5 cut(s) 41, 364, 415, 1542, 2646
EarI CTCTTC 5 cut(s) 41, 364, 415, 1542, 2646
Eco105I TACGTA 1 cut(s) 1913
Eco130I CCWWGG 3 cut(s) 192, 348, 2004
Eco147I AGGCCT 1 cut(s) 1893
Eco24I GRGCYC 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 690
Eco57I CTGAAG 9 cut(s) 291, 540, 678, 798, 933, 942, 1041, 1752, 2046
EcoRI GAATTC 2 cut(s) 1249, 1933
EcoRII CCWGG 5 cut(s) 699, 1888, 2112, 2206, 2256
EcoT14I CCWWGG 3 cut(s) 192, 348, 2004
EcoT22I ATGCAT 2 cut(s) 328, 599
EcoT38I GRGCYC 1 cut(s) 427
ErhI CCWWGG 3 cut(s) 192, 348, 2004
FaeI CATG 8 cut(s) 326, 597, 601, 1533, 2053, 2084, 2166, 2340
FalI AAGNNNNNCTT 4 cut(s) 2624, 2656, 2643, 2675
FaqI GGGAC 3 cut(s) 565, 1014, 1212
FatI CATG 8 cut(s) 322, 593, 597, 1529, 2049, 2080, 2162, 2336
FauI CCCGC 3 cut(s) 434, 465, 695
FblI GTMKAC 1 cut(s) 2036
Fnu4HI GCNGC 1 cut(s) 2444
FokI GGATG 7 cut(s) 443, 817, 1118, 1316, 1373, 1818, 2403
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 1 cut(s) 2444
FspBI CTAG 9 cut(s) 942, 1046, 1244, 1667, 1718, 1977, 2331, 2612, 2782
GluI GCNGC 1 cut(s) 2444
GsaI CCCAGC 1 cut(s) 1648
HaeIII GGCC 2 cut(s) 1893, 2346
HapII CCGG 2 cut(s) 2156, 2413
HgaI GACGC 1 cut(s) 2678
Hin1II CATG 8 cut(s) 326, 597, 601, 1533, 2053, 2084, 2166, 2340
HincII GTYRAC 1 cut(s) 2037
HindII GTYRAC 1 cut(s) 2037
HindIII AAGCTT 1 cut(s) 450
HpaII CCGG 2 cut(s) 2156, 2413
HphI GGTGA 3 cut(s) 401, 1994, 2145
Hpy166II GTNNAC 7 cut(s) 648, 1126, 1324, 1411, 2037, 2126, 2267
Hpy8I GTNNAC 7 cut(s) 648, 1126, 1324, 1411, 2037, 2126, 2267
HpyAV CCTTC 8 cut(s) 612, 698, 820, 1162, 2003, 2459, 2750, 2756
HpyCH4III ACNGT 7 cut(s) 1123, 1321, 1512, 1817, 2405, 2605, 2677
HpyCH4IV ACGT 3 cut(s) 1173, 1912, 2263
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 1707, 2572
HpyF3I CTNAG 8 cut(s) 405, 416, 540, 692, 1905, 1929, 2055, 2665
HpySE526I ACGT 3 cut(s) 1173, 1912, 2263
Hsp92II CATG 8 cut(s) 326, 597, 601, 1533, 2053, 2084, 2166, 2340
KpnI GGTACC 1 cut(s) 2162
Kzo9I GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
LguI GCTCTTC 2 cut(s) 364, 415
LmnI GCTCC 2 cut(s) 121, 505
Lsp1109I GCAGC 1 cut(s) 2430
MabI ACCWGGT 1 cut(s) 2206
MaeI CTAG 9 cut(s) 942, 1046, 1244, 1667, 1718, 1977, 2331, 2612, 2782
MaeII ACGT 3 cut(s) 1173, 1912, 2263
MalI GATC 7 cut(s) 15, 642, 719, 1791, 2031, 2215, 2255
MboI GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
MflI RGATCY 2 cut(s) 1789, 2029
MhlI GDGCHC 6 cut(s) 376, 427, 625, 1644, 1717, 2300
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 4 cut(s) 560, 818, 1359, 2512
Mph1103I ATGCAT 2 cut(s) 328, 599
MroXI GAANNNNTTC 1 cut(s) 1603
MseI TTAA 4 cut(s) 87, 1820, 1836, 1877
MslI CAYNNNNRTG 3 cut(s) 381, 630, 885
MspCI CTTAAG 1 cut(s) 86
MspI CCGG 2 cut(s) 2156, 2413
MspR9I CCNGG 5 cut(s) 701, 1890, 2114, 2208, 2258
Mva1269I GAATGC 4 cut(s) 1008, 1206, 1586, 2626
MvaI CCWGG 5 cut(s) 701, 1890, 2114, 2208, 2258
MwoI GCNNNNNNNGC 3 cut(s) 38, 1707, 2572
NdeII GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
NlaIII CATG 8 cut(s) 326, 597, 601, 1533, 2053, 2084, 2166, 2340
NlaIV GGNNCC 6 cut(s) 189, 1113, 1311, 1641, 2160, 2411
NmeAIII GCCGAG 2 cut(s) 467, 2529
NmuCI GTSAC 5 cut(s) 330, 1152, 1350, 1437, 1662
NsiI ATGCAT 2 cut(s) 328, 599
NspI RCATGY 2 cut(s) 326, 2053
NspV TTCGAA 1 cut(s) 2485
PaqCI CACCTGC 2 cut(s) 73, 2437
PceI AGGCCT 1 cut(s) 1893
PciSI GCTCTTC 2 cut(s) 364, 415
PctI GAATGC 4 cut(s) 1008, 1206, 1586, 2626
PdmI GAANNNNTTC 1 cut(s) 1603
PflMI CCANNNNNTGG 1 cut(s) 767
PfoI TCCNGGA 1 cut(s) 2256
PinAI ACCGGT 1 cut(s) 2155
PkrI GCNGC 1 cut(s) 2445
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
Ppu21I YACGTR 1 cut(s) 1913
PsiI TTATAA 3 cut(s) 26, 744, 2070
Psp6I CCWGG 5 cut(s) 699, 1888, 2112, 2206, 2256
PspFI CCCAGC 1 cut(s) 1644
PspGI CCWGG 5 cut(s) 699, 1888, 2112, 2206, 2256
PspN4I GGNNCC 6 cut(s) 189, 1113, 1311, 1641, 2160, 2411
PstI CTGCAG 2 cut(s) 1258, 2448
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 2 cut(s) 1789, 2029
RsaI GTAC 4 cut(s) 150, 1767, 2104, 2160
RsaNI GTAC 4 cut(s) 149, 1766, 2103, 2159
RseI CAYNNNNRTG 3 cut(s) 381, 630, 885
SalI GTCGAC 1 cut(s) 2035
SapI GCTCTTC 2 cut(s) 364, 415
SaqAI TTAA 4 cut(s) 87, 1820, 1836, 1877
SatI GCNGC 1 cut(s) 2444
Sau3AI GATC 7 cut(s) 13, 640, 717, 1789, 2029, 2213, 2253
ScaI AGTACT 1 cut(s) 150
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 5 cut(s) 701, 1890, 2114, 2208, 2258
SduI GDGCHC 6 cut(s) 376, 427, 625, 1644, 1717, 2300
SexAI ACCWGGT 1 cut(s) 2206
SfcI CTRYAG 4 cut(s) 34, 1254, 1593, 2444
SfuI TTCGAA 1 cut(s) 2485
SmiMI CAYNNNNRTG 3 cut(s) 381, 630, 885
SmlI CTYRAG 1 cut(s) 86
SmoI CTYRAG 1 cut(s) 86
SnaBI TACGTA 1 cut(s) 1913
SseBI AGGCCT 1 cut(s) 1893
SsiI CCGC 3 cut(s) 427, 472, 688
SspI AATATT 1 cut(s) 1483
SspMI CTAG 9 cut(s) 942, 1046, 1244, 1667, 1718, 1977, 2331, 2612, 2782
StuI AGGCCT 1 cut(s) 1893
StyD4I CCNGG 5 cut(s) 699, 1888, 2112, 2206, 2256
StyI CCWWGG 3 cut(s) 192, 348, 2004
TaaI ACNGT 7 cut(s) 1123, 1321, 1512, 1817, 2405, 2605, 2677
TaiI ACGT 3 cut(s) 1176, 1915, 2266
TaqI TCGA 4 cut(s) 129, 1658, 2036, 2485
TaqII GACCGA 1 cut(s) 2206
TatI WGTACW 2 cut(s) 148, 2102
Tru1I TTAA 4 cut(s) 87, 1820, 1836, 1877
Tru9I TTAA 4 cut(s) 87, 1820, 1836, 1877
TscAI CASTG 4 cut(s) 1107, 1305, 1717, 2256
TseFI GTSAC 5 cut(s) 330, 1152, 1350, 1437, 1662
TseI GCWGC 1 cut(s) 2443
Tsp45I GTSAC 5 cut(s) 330, 1152, 1350, 1437, 1662
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 4 cut(s) 1107, 1305, 1717, 2256
Van91I CCANNNNNTGG 1 cut(s) 767
Vha464I CTTAAG 1 cut(s) 86
XapI RAATTY 8 cut(s) 1249, 1849, 1933, 1990, 1998, 2086, 2360, 2718
XbaI TCTAGA 3 cut(s) 941, 1045, 1243
XceI RCATGY 2 cut(s) 326, 2053
XcmI CCANNNNNNNNNTGG 3 cut(s) 346, 595, 850
XmiI GTMKAC 1 cut(s) 2036
XmnI GAANNNNTTC 1 cut(s) 1603
XspI CTAG 9 cut(s) 942, 1046, 1244, 1667, 1718, 1977, 2331, 2612, 2782
ZrmI AGTACT 1 cut(s) 150
Zsp2I ATGCAT 2 cut(s) 328, 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.