Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000439

Physical Location & Seq

Genomic Coordinates Reverse (-)

31119965 .. 31125573

Gene Structure 5609 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1231900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1740 bp

ATGTCTGGTAACCCGAAAGTTGTAAACGCGTTTCGTGTGACGAGGGCCTTGGGGATTCCTGATGCAGAGATCAAACCGGTCCTGAAGGGTTTGCTGCGAATGTATGATAAAAAGTGGGAGCTTATAGAAGAAGACAACTACAGGACTCTTATAGATGCATACTTTGAGTCTAAGGAAAACAAGGGAAAAGAAGAGAAAAGAGAAGCTGCTACAGAGCTTGATGGGTATGAAAGACCAGGAAAAAGGCTGCATTTGGCCAATGGCAGAGCTGCTGTGGACCAAGATCATGGAAGCAGAACCATGGAGTCACCCAAACAGTGTTTAACCAATGGCAGAGCTGGATGTAGTTCTCAACACCCCCTCAAACAGCCCAATGACAGAGGAAAGAAACCAATTGCACCTCATTTGGTCTCTGGAGATAGAAAAATTTCAATTCGTAATCACAAGAAAGACTTGGTGATTCCAAAAACAGAGGAAGGCACAGATAGCATTTCATCTTCTTTCGAATGTGCAGTACCGGTGAGACGTCCAGCTCTTCCAGATTTGTCTCGAGGTTCAACTGGGGGTAACTCTGGATCATCCTCTACTGAAGTGGGTAGAAGTGCTTGTGACCTCACTTGTGGGGGTAACCATGTTTCAAGACCGAAGAATCGGCAATGTGGAACAGAACATTCACAAGCTGATTTAAATGGTGTGGATTCAAGCTGTCCCATCACTCATGAGGGCAGGAAGCCAGTCAATTTTTTCCATGACATTACGAAATCTATGGAGAAAGTGAAAATATCATTGGTAGATGAAACTGGAAATGAGTGCCTGCCAAAGTTCAATTATATACCAAACAATATTATCTACCAAAATGCAAATGTGAATATCTCACTAGCTCGGATTTCAGATGAAGATTGCTGCACTGACTGCTCTGGAGATTGTCTTTCAGCGGCAATACCATGTGCATGTGCTCGTGAAACTGGTGGGGAGTTTGTTTACACACCACAAGGCCTCCTAAAAGAAGATTTTTTAGCAGCTTGTGTCAAGGAACCGGAAGAGCACAACTTTGTTTATTGTCAAGACTGTCCAAATGAGAGGTCCAAGAACGAGTACTACCCTGAAAAATGCAAAGGTCACCACATCAGGAAGTTTATAAAAGAATGCTGGAGAAAATGTGGATGTGACTTGTCGTGTGGAAATCGGGTAGTGCAAAGGGGCATTTCTTACAAGTTACAGGTATTCGTGACTCCTGAAGGGAAGGGCTGGGGTGTTAGGACATTAGAGGTCTTGCAGAAGGGAACATTTGTCTGTGAATATGTTGGGGAGATACTGACCAACACCGAATTATACGAGAGAAATAACCAAAGCAGTGGAGCTAAGAGACATACCTACCCCGTATTGCTAGACGGAGATTGGGGCTCAGAGCAAATTTTGAAGGATGATGATGCCCTTTGTCTTGATGCAACATCTCATGGTAATGTTGCCAGATTTATCAATCATAGATGCTCTGATGCAAACTTGATTGATATCCCAGTTCAAGTGGAGACTCCTGACCGTCACTACTATCACCTTGCCTTTTTTACTACCAGGAAAGTGAATGCATTTGAAGAGCTGTCGTGGGATTATGGTATTGACTTTGATGATCATGGACACCCTATAGAGGCGTTTTCGTGTAATTGTGGTAGTGTAGCTTGCCGGGGAAAGAAACAGAACGGACGGAAAGAACGCAGAGGGAAAAGATTGGTGTTGGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005911 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009506 GO:0009987 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019538 GO:0030054 GO:0031974 GO:0031981 GO:0032259 GO:0034968 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0051276 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pre-SET SET WIYLD

Protein Analysis

580

Amino Acids

64.63

Weight (kDa)

7.21

Isoelectric Point (pI)

45.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WIYLD	PF10440	4 - 60	1e-17	Ubiquitin-binding WIYLD domain
Pre-SET	PF05033	254 - 396	2.5e-10	Pre-SET motif
SET	PF00856	416 - 538	2.8e-18	SET domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000439)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380
fragaria_vesca	FvH4_2g02250 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_5g31170 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620
malus_domestica	MD02G1265700.v1.1 MD04G1231900.v1.1 MD12G1043600.v1.1 MD12G1250000.v1.1
prunus_persica	Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1
pyrus_communis	pycom02g22720 pycom04g20520
rosa_chinensis	RchiOBHm_Chr3g0449441 RchiOBHm_Chr4g0395181 RchiOBHm_Chr6g0245941 RchiOBHm_Chr7g0228041
rosa_laevigata	RLG00000001593 RLG00000009641 RLG00000015284 RLG00000025830
rosa_multiflora	Rmu_co8250301.1_g000001 Rmu_co8518901.1_g000001 Rmu_sc0004858.1_g000011 Rmu_sc0007224.1_g000005 Rmu_sc0007891.1_g000010 Rmu_sc0019478.1_g000002 Rmu_sc0024829.1_g000001 Rmu_sc0039188.1_g000001
rosa_roxburghii	Rroxscaffold_3G00231620 Rroxscaffold_5G00340120 Rroxscaffold_6G00427390 Rroxscaffold_7G00215050
rosa_rugosa	Rorug02G0620600 Rorug03G0359100.1 Rorug03G0359200.1 Rorug03G0359300.1 Rorug05G0517600 Rorug05G0517700 Rorug05G0517800 Rorug05G0517900 Rorug05G0518000 Rorug05G0518100 Rorug05G0518200 Rorug05G0518300.1 Rorug07G0245600
rosa_samantha	Rh3AG018400 Rh3BG018800 Rh3CG017700 Rh4AG057300 Rh4BG056200 Rh4CG062300 Rh4DG053200 Rh6AG032500 Rh6BG028300 Rh6CG028300 Rh6CG028500 Rh6CG028600 Rh7AG399400 Rh7BG380800 Rh7CG417400 Rh7DG394900
rosa_wichuraiana	Rw4G004610 Rw6G002670 Rw7G033190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1139
AatII	GACGTC	1 cut(s)	529
AccII	CGCG	1 cut(s)	29
AciI	CCGC	1 cut(s)	935
AclWI	GGATC	1 cut(s)	583
AcoI	YGGCCR	1 cut(s)	255
AcsI	RAATTY	2 cut(s)	426, 1413
AcuI	CTGAAG	3 cut(s)	104, 609, 1257
AcyI	GRCGYC	1 cut(s)	526
AfaI	GTAC	2 cut(s)	516, 1097
AfiI	CCNNNNNNNGG	1 cut(s)	1645
AflIII	ACRYGT	1 cut(s)	27
AgeI	ACCGGT	2 cut(s)	76, 517
AgsI	TTSAA	8 cut(s)	432, 558, 639, 702, 826, 1420, 1523, 1592
AjnI	CCWGG	2 cut(s)	235, 1571
AjuI	GAANNNNNNNTTGG	2 cut(s)	770, 802
Alw21I	GWGCWC	2 cut(s)	958, 1047
Alw26I	GTCTC	5 cut(s)	415, 517, 552, 1360, 1523
AlwI	GGATC	1 cut(s)	583
Ama87I	CYCGRG	1 cut(s)	549
AoxI	GGCC	3 cut(s)	45, 255, 994
ApeKI	GCWGC	6 cut(s)	94, 206, 247, 269, 903, 1019
ApoI	RAATTY	2 cut(s)	426, 1413
ArsI	GACNNNNNNTTYG	2 cut(s)	1067, 1099
AsiGI	ACCGGT	2 cut(s)	76, 517
Asp700I	GAANNNNTTC	1 cut(s)	427
AspS9I	GGNCC	4 cut(s)	45, 79, 277, 1083
AsuC2I	CCSGG	1 cut(s)	1682
AsuHPI	GGTGA	5 cut(s)	300, 469, 532, 1112, 1544
AsuII	TTCGAA	1 cut(s)	504
AvaI	CYCGRG	1 cut(s)	549
AvaII	GGWCC	3 cut(s)	79, 277, 1083
BalI	TGGCCA	1 cut(s)	257
BanII	GRGCYC	1 cut(s)	1406
BauI	CACGAG	1 cut(s)	957
BbsI	GAAGAC	1 cut(s)	138
Bbv12I	GWGCWC	2 cut(s)	958, 1047
BbvI	GCAGC	6 cut(s)	81, 193, 234, 256, 890, 1031
BccI	CCATC	2 cut(s)	215, 719
BciT130I	CCWGG	2 cut(s)	237, 1573
BclI	TGATCA	1 cut(s)	1627
BcnI	CCSGG	1 cut(s)	1682
BcoDI	GTCTC	5 cut(s)	415, 517, 552, 1360, 1523
BfaI	CTAG	2 cut(s)	878, 1388
BfmI	CTRYAG	3 cut(s)	139, 210, 1641
BisI	GCNGC	7 cut(s)	95, 207, 248, 270, 904, 936, 1020
BlsI	GCNGC	7 cut(s)	96, 208, 249, 271, 905, 937, 1021
BmcAI	AGTACT	1 cut(s)	1097
Bme1390I	CCNGG	3 cut(s)	237, 1573, 1682
Bme18I	GGWCC	3 cut(s)	79, 277, 1083
BmeT110I	CYCGRG	1 cut(s)	549
BmgT120I	GGNCC	4 cut(s)	45, 79, 277, 1083
BmiI	GGNNCC	1 cut(s)	1035
BmrFI	CCNGG	3 cut(s)	237, 1573, 1682
BmrI	ACTGGG	2 cut(s)	570, 1511
BmsI	GCATC	6 cut(s)	52, 145, 1420, 1435, 1478, 1486
BmuI	ACTGGG	2 cut(s)	570, 1511
BpiI	GAAGAC	1 cut(s)	138
BpmI	CTGGAG	3 cut(s)	435, 939, 1171
Bpu14I	TTCGAA	1 cut(s)	504
BpuMI	CCSGG	1 cut(s)	1682
BsaBI	GATNNNNATC	1 cut(s)	1511
BsaHI	GRCGYC	1 cut(s)	526
BsaI	GGTCTC	1 cut(s)	415
BsaJI	CCNNGG	3 cut(s)	48, 300, 1681
BsaWI	WCCGGW	3 cut(s)	76, 517, 1036
BsaXI	ACNNNNNCTCC	4 cut(s)	981, 1011, 1144, 1174
Bsc4I	CCNNNNNNNGG	1 cut(s)	1645
Bse118I	RCCGGY	2 cut(s)	76, 517
Bse1I	ACTGG	5 cut(s)	565, 734, 805, 970, 1517
Bse3DI	GCAATG	1 cut(s)	662
Bse8I	GATNNNNATC	1 cut(s)	1511
BseBI	CCWGG	2 cut(s)	237, 1573
BseDI	CCNNGG	3 cut(s)	48, 300, 1681
BseGI	GGATG	4 cut(s)	347, 578, 1169, 1429
BseJI	GATNNNNATC	1 cut(s)	1511
BseLI	CCNNNNNNNGG	1 cut(s)	1645
BseMI	GCAATG	1 cut(s)	662
BseMII	CTCAG	1 cut(s)	1419
BseNI	ACTGG	5 cut(s)	565, 734, 805, 970, 1517
BseXI	GCAGC	6 cut(s)	81, 193, 234, 256, 890, 1031
BseYI	CCCAGC	1 cut(s)	1248
BsgI	GTGCAG	2 cut(s)	531, 889
Bsh1236I	CGCG	1 cut(s)	29
BshFI	GGCC	3 cut(s)	47, 257, 996
BshTI	ACCGGT	2 cut(s)	76, 517
BsiHKAI	GWGCWC	2 cut(s)	958, 1047
BsiHKCI	CYCGRG	1 cut(s)	549
BsiSI	CCGG	4 cut(s)	77, 518, 1037, 1681
BslFI	GGGAC	1 cut(s)	693
BslI	CCNNNNNNNGG	1 cut(s)	1645
BsmAI	GTCTC	5 cut(s)	415, 517, 552, 1360, 1523
BsmBI	CGTCTC	1 cut(s)	517
BsmFI	GGGAC	1 cut(s)	693
BsmI	GAATGC	2 cut(s)	1151, 1588
BsnI	GGCC	3 cut(s)	47, 257, 996
Bso31I	GGTCTC	1 cut(s)	415
BsoBI	CYCGRG	1 cut(s)	549
Bsp119I	TTCGAA	1 cut(s)	504
Bsp1286I	GDGCHC	3 cut(s)	958, 1047, 1406
Bsp143I	GATC	4 cut(s)	69, 283, 575, 1627
Bsp19I	CCATGG	1 cut(s)	300
BspACI	CCGC	1 cut(s)	935
BspANI	GGCC	3 cut(s)	47, 257, 996
BspCNI	CTCAG	1 cut(s)	1418
BspFNI	CGCG	1 cut(s)	29
BspHI	TCATGA	1 cut(s)	718
BspLI	GGNNCC	1 cut(s)	1035
BspPI	GGATC	1 cut(s)	583
BspQI	GCTCTTC	3 cut(s)	540, 1035, 1587
BspT104I	TTCGAA	1 cut(s)	504
BspTNI	GGTCTC	1 cut(s)	415
BsrDI	GCAATG	1 cut(s)	662
BsrFI	RCCGGY	2 cut(s)	76, 517
BsrI	ACTGG	5 cut(s)	565, 734, 805, 970, 1517
BssAI	RCCGGY	2 cut(s)	76, 517
BssECI	CCNNGG	3 cut(s)	48, 300, 1681
BssMI	GATC	4 cut(s)	69, 283, 575, 1627
BssNI	GRCGYC	1 cut(s)	526
BssSI	CACGAG	1 cut(s)	957
BssT1I	CCWWGG	2 cut(s)	48, 300
Bst2BI	CACGAG	1 cut(s)	957
Bst2UI	CCWGG	2 cut(s)	237, 1573
Bst4CI	ACNGT	3 cut(s)	318, 1070, 1541
Bst6I	CTCTTC	4 cut(s)	186, 540, 1035, 1587
BstACI	GRCGYC	1 cut(s)	526
BstAPI	GCANNNNNTGC	1 cut(s)	912
BstBI	TTCGAA	1 cut(s)	504
BstC8I	GCNNGC	2 cut(s)	815, 1678
BstDEI	CTNAG	3 cut(s)	171, 1362, 1405
BstDSI	CCRYGG	1 cut(s)	300
BstEII	GGTNACC	3 cut(s)	8, 626, 1118
BstF5I	GGATG	4 cut(s)	347, 578, 1169, 1429
BstFNI	CGCG	1 cut(s)	29
BstKTI	GATC	4 cut(s)	72, 286, 578, 1630
BstMAI	GTCTC	5 cut(s)	415, 517, 552, 1360, 1523
BstMBI	GATC	4 cut(s)	69, 283, 575, 1627
BstMWI	GCNNNNNNNGC	2 cut(s)	486, 912
BstNI	CCWGG	2 cut(s)	237, 1573
BstNSI	RCATGY	1 cut(s)	954
BstPI	GGTNACC	3 cut(s)	8, 626, 1118
BstSCI	CCNGG	3 cut(s)	235, 1571, 1680
BstSFI	CTRYAG	3 cut(s)	139, 210, 1641
BstUI	CGCG	1 cut(s)	29
BstV1I	GCAGC	6 cut(s)	81, 193, 234, 256, 890, 1031
BstV2I	GAAGAC	1 cut(s)	138
BstXI	CCANNNNNNTGG	2 cut(s)	287, 1355
BsuRI	GGCC	3 cut(s)	47, 257, 996
BtgI	CCRYGG	1 cut(s)	300
BtsCI	GGATG	4 cut(s)	347, 578, 1169, 1429
BtsI	GCAGTG	1 cut(s)	1360
BtsIMutI	CAGTG	3 cut(s)	323, 906, 1360
Cac8I	GCNNGC	2 cut(s)	815, 1678
CciI	TCATGA	1 cut(s)	718
Cfr10I	RCCGGY	2 cut(s)	76, 517
Cfr13I	GGNCC	4 cut(s)	45, 79, 277, 1083
Csp6I	GTAC	2 cut(s)	515, 1096
CspAI	ACCGGT	2 cut(s)	76, 517
CviAII	CATG	9 cut(s)	287, 301, 632, 719, 749, 945, 951, 1457, 1631
CviQI	GTAC	2 cut(s)	515, 1096
DdeI	CTNAG	3 cut(s)	171, 1362, 1405
DpnI	GATC	4 cut(s)	71, 285, 577, 1629
DpnII	GATC	4 cut(s)	69, 283, 575, 1627
DraI	TTTAAA	1 cut(s)	687
EaeI	YGGCCR	1 cut(s)	255
Eam1104I	CTCTTC	4 cut(s)	186, 540, 1035, 1587
EarI	CTCTTC	4 cut(s)	186, 540, 1035, 1587
Eco130I	CCWWGG	2 cut(s)	48, 300
Eco147I	AGGCCT	1 cut(s)	996
Eco24I	GRGCYC	1 cut(s)	1406
Eco31I	GGTCTC	1 cut(s)	415
Eco32I	GATATC	1 cut(s)	1513
Eco47I	GGWCC	3 cut(s)	79, 277, 1083
Eco57I	CTGAAG	3 cut(s)	104, 609, 1257
Eco88I	CYCGRG	1 cut(s)	549
Eco91I	GGTNACC	3 cut(s)	8, 626, 1118
EcoO109I	RGGNCCY	1 cut(s)	45
EcoO65I	GGTNACC	3 cut(s)	8, 626, 1118
EcoRII	CCWGG	2 cut(s)	235, 1571
EcoRV	GATATC	1 cut(s)	1513
EcoT14I	CCWWGG	2 cut(s)	48, 300
EcoT22I	ATGCAT	2 cut(s)	160, 1588
EcoT38I	GRGCYC	1 cut(s)	1406
ErhI	CCWWGG	2 cut(s)	48, 300
Esp3I	CGTCTC	1 cut(s)	517
FaeI	CATG	9 cut(s)	290, 304, 635, 722, 752, 948, 954, 1460, 1634
FalI	AAGNNNNNCTT	2 cut(s)	437, 469
FaqI	GGGAC	1 cut(s)	693
FatI	CATG	9 cut(s)	286, 300, 631, 718, 748, 944, 950, 1456, 1630
FbaI	TGATCA	1 cut(s)	1627
Fnu4HI	GCNGC	7 cut(s)	95, 207, 248, 270, 904, 936, 1020
FokI	GGATG	4 cut(s)	354, 565, 1176, 1436
FriOI	GRGCYC	1 cut(s)	1406
Fsp4HI	GCNGC	7 cut(s)	95, 207, 248, 270, 904, 936, 1020
FspBI	CTAG	2 cut(s)	878, 1388
GluI	GCNGC	7 cut(s)	95, 207, 248, 270, 904, 936, 1020
GsaI	CCCAGC	1 cut(s)	1252
GsuI	CTGGAG	3 cut(s)	435, 939, 1171
HaeIII	GGCC	3 cut(s)	47, 257, 996
HapII	CCGG	4 cut(s)	77, 518, 1037, 1681
Hin1I	GRCGYC	1 cut(s)	526
Hin1II	CATG	9 cut(s)	290, 304, 635, 722, 752, 948, 954, 1460, 1634
HinfI	GANTC	9 cut(s)	55, 145, 167, 305, 460, 649, 698, 1231, 1531
HpaII	CCGG	4 cut(s)	77, 518, 1037, 1681
HphI	GGTGA	5 cut(s)	300, 469, 532, 1112, 1544
Hpy166II	GTNNAC	3 cut(s)	25, 277, 982
Hpy188I	TCNGA	4 cut(s)	885, 892, 1408, 1495
Hpy8I	GTNNAC	3 cut(s)	25, 277, 982
HpyAV	CCTTC	6 cut(s)	79, 470, 1232, 1237, 1273, 1414
HpyCH4III	ACNGT	3 cut(s)	318, 1070, 1541
HpyCH4IV	ACGT	1 cut(s)	526
HpyF10VI	GCNNNNNNNGC	2 cut(s)	486, 912
HpyF3I	CTNAG	3 cut(s)	171, 1362, 1405
HpySE526I	ACGT	1 cut(s)	526
Hsp92I	GRCGYC	1 cut(s)	526
Hsp92II	CATG	9 cut(s)	290, 304, 635, 722, 752, 948, 954, 1460, 1634
Ksp22I	TGATCA	1 cut(s)	1627
Kzo9I	GATC	4 cut(s)	69, 283, 575, 1627
LguI	GCTCTTC	3 cut(s)	540, 1035, 1587
LmnI	GCTCC	2 cut(s)	118, 1358
Lsp1109I	GCAGC	6 cut(s)	81, 193, 234, 256, 890, 1031
LweI	GCATC	6 cut(s)	52, 145, 1420, 1435, 1478, 1486
MaeI	CTAG	2 cut(s)	878, 1388
MaeII	ACGT	1 cut(s)	526
MalI	GATC	4 cut(s)	71, 285, 577, 1629
MboI	GATC	4 cut(s)	69, 283, 575, 1627
MfeI	CAATTG	1 cut(s)	393
MhlI	GDGCHC	3 cut(s)	958, 1047, 1406
MlsI	TGGCCA	1 cut(s)	257
MluCI	AATT	8 cut(s)	393, 426, 432, 739, 826, 1328, 1413, 1660
MluI	ACGCGT	1 cut(s)	27
MluNI	TGGCCA	1 cut(s)	257
MlyI	GAGTC	5 cut(s)	139, 176, 314, 1225, 1525
Mox20I	TGGCCA	1 cut(s)	257
Mph1103I	ATGCAT	2 cut(s)	160, 1588
MroXI	GAANNNNTTC	1 cut(s)	427
MscI	TGGCCA	1 cut(s)	257
MseI	TTAA	2 cut(s)	323, 686
MslI	CAYNNNNRTG	2 cut(s)	949, 1461
Msp20I	TGGCCA	1 cut(s)	257
MspA1I	CMGCKG	1 cut(s)	935
MspI	CCGG	4 cut(s)	77, 518, 1037, 1681
MspR9I	CCNGG	3 cut(s)	237, 1573, 1682
MunI	CAATTG	1 cut(s)	393
Mva1269I	GAATGC	2 cut(s)	1151, 1588
MvaI	CCWGG	2 cut(s)	237, 1573
MvnI	CGCG	1 cut(s)	29
MwoI	GCNNNNNNNGC	2 cut(s)	486, 912
NciI	CCSGG	1 cut(s)	1682
NcoI	CCATGG	1 cut(s)	300
NdeII	GATC	4 cut(s)	69, 283, 575, 1627
NlaIII	CATG	9 cut(s)	290, 304, 635, 722, 752, 948, 954, 1460, 1634
NlaIV	GGNNCC	1 cut(s)	1035
NmuCI	GTSAC	7 cut(s)	37, 306, 608, 1118, 1166, 1228, 1541
NsiI	ATGCAT	2 cut(s)	160, 1588
NspI	RCATGY	1 cut(s)	954
NspV	TTCGAA	1 cut(s)	504
PaeR7I	CTCGAG	1 cut(s)	549
PagI	TCATGA	1 cut(s)	718
PceI	AGGCCT	1 cut(s)	996
PciSI	GCTCTTC	3 cut(s)	540, 1035, 1587
PctI	GAATGC	2 cut(s)	1151, 1588
PdmI	GAANNNNTTC	1 cut(s)	427
PfeI	GAWTC	4 cut(s)	55, 460, 649, 698
PinAI	ACCGGT	2 cut(s)	76, 517
PkrI	GCNGC	7 cut(s)	96, 208, 249, 271, 905, 937, 1021
PleI	GAGTC	5 cut(s)	139, 175, 313, 1225, 1525
PpsI	GAGTC	5 cut(s)	139, 175, 313, 1225, 1525
PsiI	TTATAA	1 cut(s)	1139
Psp6I	CCWGG	2 cut(s)	235, 1571
PspEI	GGTNACC	3 cut(s)	8, 626, 1118
PspFI	CCCAGC	1 cut(s)	1248
PspGI	CCWGG	2 cut(s)	235, 1571
PspN4I	GGNNCC	1 cut(s)	1035
PspPI	GGNCC	4 cut(s)	45, 79, 277, 1083
PsrI	GAACNNNNNNTAC	2 cut(s)	1082, 1114
RsaI	GTAC	2 cut(s)	516, 1097
RsaNI	GTAC	2 cut(s)	515, 1096
RseI	CAYNNNNRTG	2 cut(s)	949, 1461
SapI	GCTCTTC	3 cut(s)	540, 1035, 1587
SaqAI	TTAA	2 cut(s)	323, 686
SatI	GCNGC	7 cut(s)	95, 207, 248, 270, 904, 936, 1020
Sau3AI	GATC	4 cut(s)	69, 283, 575, 1627
Sau96I	GGNCC	4 cut(s)	45, 79, 277, 1083
ScaI	AGTACT	1 cut(s)	1097
SchI	GAGTC	5 cut(s)	139, 176, 314, 1225, 1525
ScrFI	CCNGG	3 cut(s)	237, 1573, 1682
SduI	GDGCHC	3 cut(s)	958, 1047, 1406
SfaNI	GCATC	6 cut(s)	52, 145, 1420, 1435, 1478, 1486
SfcI	CTRYAG	3 cut(s)	139, 210, 1641
Sfr274I	CTCGAG	1 cut(s)	549
SfuI	TTCGAA	1 cut(s)	504
SinI	GGWCC	3 cut(s)	79, 277, 1083
SlaI	CTCGAG	1 cut(s)	549
SmiI	ATTTAAAT	1 cut(s)	687
SmiMI	CAYNNNNRTG	2 cut(s)	949, 1461
SmlI	CTYRAG	1 cut(s)	549
SmoI	CTYRAG	1 cut(s)	549
Sse9I	AATT	8 cut(s)	393, 426, 432, 739, 826, 1328, 1413, 1660
SseBI	AGGCCT	1 cut(s)	996
SsiI	CCGC	1 cut(s)	935
SspI	AATATT	1 cut(s)	844
SspMI	CTAG	2 cut(s)	878, 1388
StuI	AGGCCT	1 cut(s)	996
StyD4I	CCNGG	3 cut(s)	235, 1571, 1680
StyI	CCWWGG	2 cut(s)	48, 300
SwaI	ATTTAAAT	1 cut(s)	687
TaaI	ACNGT	3 cut(s)	318, 1070, 1541
TaiI	ACGT	1 cut(s)	529
TaqI	TCGA	2 cut(s)	504, 550
TaqII	GACCGA	1 cut(s)	658
TasI	AATT	8 cut(s)	393, 426, 432, 739, 826, 1328, 1413, 1660
TatI	WGTACW	1 cut(s)	1095
TauI	GCSGC	1 cut(s)	938
TfiI	GAWTC	4 cut(s)	55, 460, 649, 698
Tru1I	TTAA	2 cut(s)	323, 686
Tru9I	TTAA	2 cut(s)	323, 686
TscAI	CASTG	3 cut(s)	323, 913, 1360
TseFI	GTSAC	7 cut(s)	37, 306, 608, 1118, 1166, 1228, 1541
TseI	GCWGC	6 cut(s)	94, 206, 247, 269, 903, 1019
Tsp45I	GTSAC	7 cut(s)	37, 306, 608, 1118, 1166, 1228, 1541
TspDTI	ATGAA	4 cut(s)	243, 483, 810, 909
TspGWI	ACGGA	3 cut(s)	1407, 1713, 1717
TspRI	CASTG	3 cut(s)	323, 913, 1360
VpaK11BI	GGWCC	3 cut(s)	79, 277, 1083
XapI	RAATTY	2 cut(s)	426, 1413
XceI	RCATGY	1 cut(s)	954
XhoI	CTCGAG	1 cut(s)	549
XmnI	GAANNNNTTC	1 cut(s)	427
XspI	CTAG	2 cut(s)	878, 1388
ZraI	GACGTC	1 cut(s)	527
ZrmI	AGTACT	1 cut(s)	1097
Zsp2I	ATGCAT	2 cut(s)	160, 1588

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.