Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0000439

Physical Location & Seq

Genomic Coordinates Reverse (-)

70264199 .. 70265677

Gene Structure 1479 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0518200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGGTCTGATCGGAATCGCTTCTGATGTTTATACTCTAACCATTATCATTAATTGCTTTTGCCATTTGAATCAAATGGGGTTTAGCTTATCTGTCTTGGGGCAATACTTCAAATTGGGTCTTCAACCAGATGTCACCACCTACACTACTCTAATCAACGGCTTTGTTCTCAAGAATAAAATGGCAGAGGCAGCAGGGATTTTCAGCAAAATGCTGCAGGCAGGTCACTGTGTGCCCAATGTGGTGACTTTCAACACACTAATAAAGGGACTTTGCATGAGGGGTGACAACACTGCAGCTATTCAATTGCTCAGGAAGATGGAAGAAACAGGTCGTAAGCCTGACATATTTACCTATAGCACCATCATTGACAGTCTTTGCAAGGATACACTAGTGGTTGATGCACTGAACCTCTTCTCAGAAATGATTAGTAGGGGCGTTGCTCCAGACGTTGTTACTTACACCTCTTTGATTCAAGGAGTTTGCATCGTAGGTAAGTGGAAAGAAGCTACAAGGTTGTTGAATGAAATGGTTAGTAGAAATATCTTTCCGGATGTAGTCACCTTCAATGTCTTGGTTGATGGACTTTGTAAGGAGGGGATGGTTGTGGAAGCCAAAAGTGTGGTTGAAATGATGATCCCAAGAGATATTGAGCCTAATGCAATTACTTACAATTCACTAATGGATGGTTACTGTTTGCGAGGTGAAATGGATGAAGCAAGAAAAGTCTTTGATCTAATGCTTAGCAAGGCCTGCATGGTTAATGTTCGTAGTTGTAGTATATTGATAAATGGATACTGTAAACATAAAAAGATTGATGAGGCCAACAAGGTTTTTCAGGAAATGCTTTGTATGGAACTTGTTCCAGATACCATTACTTACAATACTCTTATTGACGGTTTTTGTAAAGCAGGAAGAATACGGGTAGCAGAGAAGTTGTTTTCTCAGATGCAAGGTTGTGGTCAACTTCCAGATGTTCAAACTTACGCCATTCTAATGAATGGCCTATGTGGAAACAAGCAACTTTCAACGGCAGTGGAACTGCTTAGAGAGATGGAAGATAACAAGTTGGAAGTAAGTATCATAATTTACACCATCATCATTGAAGGTTTGTGCAAAGCTGGAAAACTTGAATCTGCAACAGATGTCTTCTCTAGTTTGTCTTCAAAAAGAGTTCAGCTTGATGTGAAGGCATATACTGTGATGATTAATGGACTCTGTGATGGACGCCTATTGGTTGAAGCAGAAAAGTTGCTTAGAGAAATGGTAGAGAGAGGCTGTTCTCCAAATGATTGTACATATAACACCATCATCCGAGGGTTTATCAATAACAATGAGACATCAAGGGCTGTGGGACTAATTCAAGAAATGGTGGAGATGGGGTTCTCTGCAGATGCATCAACTGCAGAATTGATAATTGATTTGTTGTCTAAAGATAAAGTTGATCCAGCTTTGTTGTCATTGTTAAAGAAGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005911 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009506 GO:0009987 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019538 GO:0030054 GO:0031974 GO:0031981 GO:0032259 GO:0034968 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0051276 GO:0055044 GO:0070013 GO:0071704 GO:0071840 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pre-SET SET WIYLD

Protein Analysis

492

Amino Acids

54.43

Weight (kDa)

5.36

Isoelectric Point (pI)

26.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	38 - 89	5.5e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	40 - 71	1.8e-07	PPR repeat
PPR_1	PF12854	77 - 108	9.6e-12	PPR repeat
PPR_2	PF13041	79 - 128	1.3e-17	PPR repeat family
PPR_3	PF13812	79 - 124	8.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	111 - 142	1.6e-10	PPR repeat
PPR	PF01535	117 - 147	5.3e-06	PPR repeat
PPR_2	PF13041	121 - 162	1.7e-12	PPR repeat family
PPR_long	PF17177	132 - 228	5e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	138 - 193	1.8e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	145 - 178	2.7e-11	PPR repeat
PPR_2	PF13041	149 - 198	7.1e-19	PPR repeat family
PPR	PF01535	152 - 182	1.4e-06	PPR repeat
PPR_1	PF12854	180 - 212	3.5e-11	PPR repeat
PPR_2	PF13041	184 - 232	7.4e-15	PPR repeat family
PPR_1	PF12854	216 - 247	8.9e-12	PPR repeat
PPR_2	PF13041	219 - 268	2.1e-15	PPR repeat family
PPR	PF01535	222 - 250	2e-08	PPR repeat
PPR_1	PF12854	252 - 282	2.6e-07	PPR repeat
PPR_2	PF13041	255 - 303	1.9e-16	PPR repeat family
PPR	PF01535	258 - 284	5.2e-07	PPR repeat
TPR_24	PF23276	285 - 386	2.7e-09	Fungal tetratrico peptide repeats
PPR_1	PF12854	286 - 318	5.1e-15	PPR repeat
PPR_2	PF13041	289 - 337	1.8e-18	PPR repeat family
PPR	PF01535	292 - 321	7e-09	PPR repeat
PPR_1	PF12854	321 - 353	1.6e-07	PPR repeat
PPR_2	PF13041	324 - 359	4.2e-06	PPR repeat family
PPR_1	PF12854	358 - 385	6.8e-07	PPR repeat
PPR_2	PF13041	360 - 408	7.5e-13	PPR repeat family
PPR_3	PF13812	387 - 441	2.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	392 - 423	5e-06	PPR repeat
PPR	PF01535	398 - 427	5.3e-07	PPR repeat
PPR_2	PF13041	413 - 442	1.3e-08	PPR repeat family
PPR_1	PF12854	425 - 458	7e-07	PPR repeat
PPR_2	PF13041	429 - 477	4.8e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000439)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380 AT3G04380
fragaria_vesca	FvH4_2g02250 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_4g04470 FvH4_5g31170 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620 FvH4_6g01620
malus_domestica	MD02G1265700.v1.1 MD04G1231900.v1.1 MD12G1043600.v1.1 MD12G1250000.v1.1
prunus_persica	Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.1G047100_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.2G058800_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1 Prupe.6G351300_v2.0.a1
pyrus_communis	pycom02g22720 pycom04g20520
rosa_chinensis	RchiOBHm_Chr3g0449441 RchiOBHm_Chr4g0395181 RchiOBHm_Chr6g0245941 RchiOBHm_Chr7g0228041
rosa_laevigata	RLG00000001593 RLG00000009641 RLG00000015284 RLG00000025830
rosa_multiflora	Rmu_co8250301.1_g000001 Rmu_co8518901.1_g000001 Rmu_sc0004858.1_g000011 Rmu_sc0007224.1_g000005 Rmu_sc0007891.1_g000010 Rmu_sc0019478.1_g000002 Rmu_sc0024829.1_g000001 Rmu_sc0039188.1_g000001
rosa_roxburghii	Rroxscaffold_3G00231620 Rroxscaffold_5G00340120 Rroxscaffold_6G00427390 Rroxscaffold_7G00215050
rosa_rugosa	Rorug02G0620600 Rorug03G0359100.1 Rorug03G0359200.1 Rorug03G0359300.1 Rorug05G0517600 Rorug05G0517700 Rorug05G0517800 Rorug05G0517900 Rorug05G0518000 Rorug05G0518100 Rorug05G0518200 Rorug05G0518300.1 Rorug07G0245600
rosa_samantha	Rh3AG018400 Rh3BG018800 Rh3CG017700 Rh4AG057300 Rh4BG056200 Rh4CG062300 Rh4DG053200 Rh6AG032500 Rh6BG028300 Rh6CG028300 Rh6CG028500 Rh6CG028600 Rh7AG399400 Rh7BG380800 Rh7CG417400 Rh7DG394900
rosa_wichuraiana	Rw4G004610 Rw6G002670 Rw7G033190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	212
AccIII	TCCGGA	1 cut(s)	550
AclWI	GGATC	2 cut(s)	631, 1439
AcyI	GRCGYC	1 cut(s)	1228
AdeI	CACNNNGTG	1 cut(s)	232
AfaI	GTAC	1 cut(s)	1297
AhlI	ACTAGT	1 cut(s)	391
AloI	GAACNNNNNNTCC	2 cut(s)	1367, 1399
AluBI	AGCT	6 cut(s)	87, 299, 509, 1121, 1180, 1451
AluI	AGCT	6 cut(s)	87, 299, 509, 1121, 1180, 1451
Alw26I	GTCTC	1 cut(s)	1331
AlwI	GGATC	2 cut(s)	631, 1439
Aor13HI	TCCGGA	1 cut(s)	550
AoxI	GGCC	3 cut(s)	750, 822, 1003
ApeKI	GCWGC	3 cut(s)	191, 214, 296
AseI	ATTAAT	2 cut(s)	51, 1209
Asp700I	GAANNNNTTC	3 cut(s)	19, 413, 861
AsuHPI	GGTGA	5 cut(s)	127, 256, 296, 553, 716
BaeGI	GKGCMC	1 cut(s)	237
BaeI	ACNNNNGTAYC	2 cut(s)	378, 411
BbsI	GAAGAC	3 cut(s)	113, 1141, 1155
BbvI	GCAGC	3 cut(s)	201, 203, 308
BceAI	ACGGC	2 cut(s)	175, 1047
BciVI	GTATCC	2 cut(s)	379, 788
BcoDI	GTCTC	1 cut(s)	1331
BcuI	ACTAGT	1 cut(s)	391
BfaI	CTAG	2 cut(s)	392, 1155
BfmI	CTRYAG	5 cut(s)	215, 294, 355, 1389, 1404
BfuAI	ACCTGC	1 cut(s)	212
BfuI	GTATCC	2 cut(s)	379, 788
BisI	GCNGC	3 cut(s)	192, 215, 297
BlpI	GCTNAGC	1 cut(s)	743
BlsI	GCNGC	3 cut(s)	193, 216, 298
BmsI	GCATC	5 cut(s)	391, 495, 939, 1384, 1406
BpiI	GAAGAC	3 cut(s)	113, 1141, 1155
BpmI	CTGGAG	1 cut(s)	429
Bpu10I	CCTNAGC	1 cut(s)	311
Bpu1102I	GCTNAGC	1 cut(s)	743
BpuEI	CTTGAG	1 cut(s)	155
BsaBI	GATNNNNATC	1 cut(s)	14
BsaHI	GRCGYC	1 cut(s)	1228
BsaJI	CCNNGG	1 cut(s)	1315
BsaWI	WCCGGW	1 cut(s)	550
BsaXI	ACNNNNNCTCC	2 cut(s)	1367, 1397
Bse8I	GATNNNNATC	1 cut(s)	14
BseAI	TCCGGA	1 cut(s)	550
BseDI	CCNNGG	1 cut(s)	1315
BseGI	GGATG	5 cut(s)	559, 606, 691, 718, 1311
BseJI	GATNNNNATC	1 cut(s)	14
BseMII	CTCAG	3 cut(s)	325, 432, 959
BseSI	GKGCMC	1 cut(s)	237
BseXI	GCAGC	3 cut(s)	201, 203, 308
BshFI	GGCC	3 cut(s)	752, 824, 1005
BsiSI	CCGG	1 cut(s)	551
BslFI	GGGAC	2 cut(s)	282, 1368
BsmAI	GTCTC	1 cut(s)	1331
BsmFI	GGGAC	2 cut(s)	282, 1368
BsnI	GGCC	3 cut(s)	752, 824, 1005
Bsp1286I	GDGCHC	1 cut(s)	237
Bsp13I	TCCGGA	1 cut(s)	550
Bsp1407I	TGTACA	1 cut(s)	1295
Bsp143I	GATC	4 cut(s)	9, 636, 733, 1444
Bsp1720I	GCTNAGC	1 cut(s)	743
BspANI	GGCC	3 cut(s)	752, 824, 1005
BspCNI	CTCAG	3 cut(s)	324, 431, 958
BspEI	TCCGGA	1 cut(s)	550
BspHI	TCATGA	1 cut(s)	1475
BspMAI	CTGCAG	4 cut(s)	219, 298, 1393, 1408
BspMI	ACCTGC	1 cut(s)	212
BspPI	GGATC	2 cut(s)	631, 1439
BsrGI	TGTACA	1 cut(s)	1295
BssECI	CCNNGG	1 cut(s)	1315
BssMI	GATC	4 cut(s)	9, 636, 733, 1444
BssNI	GRCGYC	1 cut(s)	1228
Bst4CI	ACNGT	6 cut(s)	230, 374, 695, 800, 899, 1201
Bst6I	CTCTTC	1 cut(s)	419
BstACI	GRCGYC	1 cut(s)	1228
BstAPI	GCANNNNNTGC	2 cut(s)	753, 1403
BstAUI	TGTACA	1 cut(s)	1295
BstC8I	GCNNGC	2 cut(s)	219, 754
BstDEI	CTNAG	6 cut(s)	311, 418, 743, 945, 1046, 1256
BstF5I	GGATG	5 cut(s)	559, 606, 691, 718, 1311
BstKTI	GATC	4 cut(s)	12, 639, 736, 1447
BstMAI	GTCTC	1 cut(s)	1331
BstMBI	GATC	4 cut(s)	9, 636, 733, 1444
BstMWI	GCNNNNNNNGC	3 cut(s)	191, 753, 1403
BstSFI	CTRYAG	5 cut(s)	215, 294, 355, 1389, 1404
BstSLI	GKGCMC	1 cut(s)	237
BstV1I	GCAGC	3 cut(s)	201, 203, 308
BstV2I	GAAGAC	3 cut(s)	113, 1141, 1155
BstXI	CCANNNNNNTGG	1 cut(s)	622
BsuI	GTATCC	2 cut(s)	379, 788
BsuRI	GGCC	3 cut(s)	752, 824, 1005
BtsCI	GGATG	5 cut(s)	559, 606, 691, 718, 1311
BtsI	GCAGTG	2 cut(s)	291, 1041
BtsIMutI	CAGTG	4 cut(s)	226, 291, 404, 1041
BveI	ACCTGC	1 cut(s)	212
Cac8I	GCNNGC	2 cut(s)	219, 754
CciI	TCATGA	1 cut(s)	1475
CseI	GACGC	1 cut(s)	1236
Csp6I	GTAC	1 cut(s)	1296
CspCI	CAANNNNNGTGG	4 cut(s)	1017, 1052, 1332, 1367
CviAII	CATG	3 cut(s)	277, 757, 1476
CviQI	GTAC	1 cut(s)	1296
DdeI	CTNAG	6 cut(s)	311, 418, 743, 945, 1046, 1256
DpnI	GATC	4 cut(s)	11, 638, 735, 1446
DpnII	GATC	4 cut(s)	9, 636, 733, 1444
DraIII	CACNNNGTG	1 cut(s)	232
Eam1104I	CTCTTC	1 cut(s)	419
EarI	CTCTTC	1 cut(s)	419
Eco147I	AGGCCT	1 cut(s)	752
EcoT22I	ATGCAT	1 cut(s)	1399
FaeI	CATG	3 cut(s)	280, 760, 1479
FaqI	GGGAC	2 cut(s)	282, 1368
FatI	CATG	3 cut(s)	276, 756, 1475
Fnu4HI	GCNGC	3 cut(s)	192, 215, 297
FokI	GGATG	5 cut(s)	566, 613, 698, 725, 1298
Fsp4HI	GCNGC	3 cut(s)	192, 215, 297
FspBI	CTAG	2 cut(s)	392, 1155
GluI	GCNGC	3 cut(s)	192, 215, 297
GsuI	CTGGAG	1 cut(s)	429
HaeIII	GGCC	3 cut(s)	752, 824, 1005
HapII	CCGG	1 cut(s)	551
HgaI	GACGC	1 cut(s)	1236
Hin1I	GRCGYC	1 cut(s)	1228
Hin1II	CATG	3 cut(s)	280, 760, 1479
HincII	GTYRAC	1 cut(s)	965
HindII	GTYRAC	1 cut(s)	965
HinfI	GANTC	5 cut(s)	15, 70, 472, 1133, 1215
HpaII	CCGG	1 cut(s)	551
HphI	GGTGA	5 cut(s)	127, 256, 296, 553, 716
Hpy166II	GTNNAC	2 cut(s)	803, 965
Hpy188I	TCNGA	6 cut(s)	9, 14, 25, 421, 948, 1316
Hpy188III	TCNNGA	9 cut(s)	172, 313, 446, 551, 839, 866, 971, 1364, 1476
Hpy8I	GTNNAC	2 cut(s)	803, 965
HpyAV	CCTTC	3 cut(s)	574, 1100, 1183
HpyCH4III	ACNGT	6 cut(s)	230, 374, 695, 800, 899, 1201
HpyCH4IV	ACGT	1 cut(s)	450
HpyF10VI	GCNNNNNNNGC	3 cut(s)	191, 753, 1403
HpyF3I	CTNAG	6 cut(s)	311, 418, 743, 945, 1046, 1256
HpySE526I	ACGT	1 cut(s)	450
Hsp92I	GRCGYC	1 cut(s)	1228
Hsp92II	CATG	3 cut(s)	280, 760, 1479
Kpn2I	TCCGGA	1 cut(s)	550
Kzo9I	GATC	4 cut(s)	9, 636, 733, 1444
LmnI	GCTCC	1 cut(s)	448
Lsp1109I	GCAGC	3 cut(s)	201, 203, 308
LweI	GCATC	5 cut(s)	391, 495, 939, 1384, 1406
MaeI	CTAG	2 cut(s)	392, 1155
MaeII	ACGT	1 cut(s)	450
MaeIII	GTNAC	7 cut(s)	133, 224, 244, 284, 454, 559, 689
MalI	GATC	4 cut(s)	11, 638, 735, 1446
MboI	GATC	4 cut(s)	9, 636, 733, 1444
MboII	GAAGA	8 cut(s)	113, 328, 335, 406, 927, 1070, 1141, 1155
MfeI	CAATTG	1 cut(s)	305
MhlI	GDGCHC	1 cut(s)	237
MluCI	AATT	9 cut(s)	52, 113, 305, 663, 673, 1086, 1359, 1409, 1416
MlyI	GAGTC	1 cut(s)	1209
MmeI	TCCRAC	1 cut(s)	1050
MnlI	CCTC	9 cut(s)	181, 273, 422, 475, 589, 695, 814, 1268, 1310
Mph1103I	ATGCAT	1 cut(s)	1399
MroI	TCCGGA	1 cut(s)	550
MroXI	GAANNNNTTC	3 cut(s)	19, 413, 861
MseI	TTAA	4 cut(s)	51, 762, 1209, 1466
MslI	CAYNNNNRTG	1 cut(s)	995
MspI	CCGG	1 cut(s)	551
MunI	CAATTG	1 cut(s)	305
MwoI	GCNNNNNNNGC	3 cut(s)	191, 753, 1403
NdeII	GATC	4 cut(s)	9, 636, 733, 1444
NlaIII	CATG	3 cut(s)	280, 760, 1479
NmuCI	GTSAC	5 cut(s)	133, 224, 244, 284, 559
NsiI	ATGCAT	1 cut(s)	1399
PagI	TCATGA	1 cut(s)	1475
PceI	AGGCCT	1 cut(s)	752
PdmI	GAANNNNTTC	3 cut(s)	19, 413, 861
PfeI	GAWTC	4 cut(s)	15, 70, 472, 1133
PkrI	GCNGC	3 cut(s)	193, 216, 298
PleI	GAGTC	1 cut(s)	1209
PpsI	GAGTC	1 cut(s)	1209
PshBI	ATTAAT	2 cut(s)	51, 1209
PstI	CTGCAG	4 cut(s)	219, 298, 1393, 1408
RsaI	GTAC	1 cut(s)	1297
RsaNI	GTAC	1 cut(s)	1296
RseI	CAYNNNNRTG	1 cut(s)	995
SaqAI	TTAA	4 cut(s)	51, 762, 1209, 1466
SatI	GCNGC	3 cut(s)	192, 215, 297
Sau3AI	GATC	4 cut(s)	9, 636, 733, 1444
SchI	GAGTC	1 cut(s)	1209
SduI	GDGCHC	1 cut(s)	237
SfaNI	GCATC	5 cut(s)	391, 495, 939, 1384, 1406
SfcI	CTRYAG	5 cut(s)	215, 294, 355, 1389, 1404
SmiMI	CAYNNNNRTG	1 cut(s)	995
SmlI	CTYRAG	1 cut(s)	170
SmoI	CTYRAG	1 cut(s)	170
SpeI	ACTAGT	1 cut(s)	391
Sse9I	AATT	9 cut(s)	52, 113, 305, 663, 673, 1086, 1359, 1409, 1416
SseBI	AGGCCT	1 cut(s)	752
SspMI	CTAG	2 cut(s)	392, 1155
StuI	AGGCCT	1 cut(s)	752
TaaI	ACNGT	6 cut(s)	230, 374, 695, 800, 899, 1201
TaiI	ACGT	1 cut(s)	453
TasI	AATT	9 cut(s)	52, 113, 305, 663, 673, 1086, 1359, 1409, 1416
TatI	WGTACW	1 cut(s)	1295
TfiI	GAWTC	4 cut(s)	15, 70, 472, 1133
Tru1I	TTAA	4 cut(s)	51, 762, 1209, 1466
Tru9I	TTAA	4 cut(s)	51, 762, 1209, 1466
TscAI	CASTG	4 cut(s)	233, 298, 411, 1041
TseFI	GTSAC	5 cut(s)	133, 224, 244, 284, 559
TseI	GCWGC	3 cut(s)	191, 214, 296
Tsp45I	GTSAC	5 cut(s)	133, 224, 244, 284, 559
TspDTI	ATGAA	3 cut(s)	540, 729, 1013
TspRI	CASTG	4 cut(s)	233, 298, 411, 1041
VspI	ATTAAT	2 cut(s)	51, 1209
XmnI	GAANNNNTTC	3 cut(s)	19, 413, 861
XspI	CTAG	2 cut(s)	392, 1155
Zsp2I	ATGCAT	1 cut(s)	1399

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.