Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001658

Physical Location & Seq

Genomic Coordinates Forward (+)

32138508 .. 32147146

Gene Structure 8639 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1267100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2913 bp

ATGGAGCAGCTCAAGACCATCGGCCGGGAACTCGCTATGGGCTCCCAGGGCGGCTTCGGCCAATCCAAGGAGTTCCTCGACCTCGTCAAGTCCATCGGCGAAGCTCGATCTAAGGCGGAGGAGGAGCGCATCGTCCTCCTCGAGATCGAAACCCTTAAACGACGTCTCTCCGAGCCCGAAATCCCCAAGCGCAAGATGAAGGAGTACATCGTCCGCCTCGTCTACGTTGAAATGCTCGGCCACGACGGCTCCTTCGCCTACATTCACGCCGTTAAGATGACTCACGACGATAACCTCCTCCTCAAGCGCACCGGTTACCTCGCCGTTTCCCTCTTCCTCAGTGACGATCACGATTTGATCATCTTGATCGTCAACACCATCCAGAAGGATCTCAGGTCTGACAATTACCTCGTCGTTTGCGCCGCCCTCAATGCCGTTTGCAAGCTCATCAACGACGAGACTGTGCCTGCCGTTTTGCCCCAGGTCGTCGACCTCCTCGCCCATCCCAAGGAGGCTGTCAGGAAGAAAGCCATCATGGCGCTTCACCGGTTTTATCAGAAATCGCCCTCCTCTGTTTCGCATCTAGTGTCGAGTTTTCGGAAGCGGCTTTGCGATAACGATCCGGGCGTCATGGGTGCCACGCTCTGGCCGCTGTTTGATCTCATTACCATCGATGTGAATTCTTATAAGGATTTGGTGGTCAGCTTTGTCAGCATTCTCAAGCAGGTCGCCGAGCGCAGGTTGCCTAAGGCCTACGATTACCATCAGCTTCCGGCGCCCTTTATTCAGATTAGGTTGTTGAAGATTTTGGCATTGTTGGGGAGTGGTGATAAGCAATCAAGTGAGAGCATGTATATGGTAGTCGGCGATATATTTCGGAAGAGCGAATCCACAAGTAATATTGGAAATGCTGTTCTTTACGAGTGCATTTGTTGCGTTTCTTCCATATATCCTAATCCTAAGTTGTTAGAACAGGCTGCTCAAGTCATCTCAAGATTTTTGAAGAGTGACAGTCACAATTTGAAATATATGGGCATTGATGCCCTTGGTCGACTGATAAAGATTAGTCCAGAGATTGCCGAGCAACACCAACTAGCCGTCATTGATTGCTTAGAGGACCCAGATGATACTCTGAAGCGAAAAACGTTTGAATTATTGTACAAGATGACCAAGTCCTCCAATGTGGAAGTAATTGTGGATCGTATGATTGATTACATGATTAGCATTAATGACAACCATTACAAAGCATACATAGCTTCTAGATGTGTGGAGCTTGCAGAGCAATTTGCACCAAGTAATCAATGGTTTATCCAGACCATGAATAAAGTCTTCGAACATGCCGGGGATCTAGTGAATGTTAAGGTGGCACATAACTTGATGAAGTTGATTGCCGAGGGATTTGGAGAGGATGATGATACTGCAGATAGTCAGTTGAGATCATCTGCGGTGGAGTCATATTTACGCATAATTGGTGAGCCAAAGCTTCCATCTGTATTTCTTCAGGTTATTTGTTGGGTTTTGGGGGAATATGGAACTGCTGATGGAAAATATTCTGCTTCCTATATCACTGGGAAGTTATGTGATGTTGCAGAGTCATATTCAAACGACGAATCCGTCAAGGCTTATGCTGTTACAGCAATCATGAAAATATATGCATTTGAAATATCCGCTCGGAGAAAAGTCGAGATCCTACCTGAGTGTCAGTCTTTGGTGGAAGAATTATCAGCTTCTCACTCAACAGATCTACAACAGCGTGCATATGAATTGCAAGCTGTCATTAGCTTAGATGCTCCTGCTGTTGAGAGTATAATGCCGCTAGACGCAAGTTGTGAAGACATTGAGATTGATAAAAACCTTTCATTCCTCAATGGCTATGTCCAAGAAGCACTAGAAAAAGGCGCTCAGCCCTATATTCCCGAGAATGAGCGCTCAGGAGTGTTAGATGTCAGCAACTTCGGAAACCAAGATCACCATGAAGCTTTAACCCATAGTCTTAGGTTTGAAGCGTATGATCTTCCAAAGCCAGTAGTGTCATCAAGAGTTCCCCCAGCTGCAGTTGCTTCCTCAACTGAGCTTGTTCCAGTGCTAGAACCATCTTATGCGAGGGAGACCCGCCAACCTGCATCATCGCCATCTGTCTCAGATGCAGGATCGTCGGAACTTAAGCTACGACTTGATGGTGTACAAAAAAAATGGGGCAGGCCAACATACTCTTCTTCTGCATCACCGAGCTCGAGCTCTTCAAGTTCTACTTCCCACAACACAACGAATGGGGTCACACAAGTAGATAGCGTGGGCACTTCAAATTCAAAAGCACGTGACACTTATGATTCAAGGCGGCCACAGGTTGAGATTTCTCAAGAAAAGCAGAAGCTTGCCTCTTCTCTGTTTGGAGGCTCATCAAAAACCGAGAAGAGGTCATCTTCTGCCAACCATAAGGCATCTAAGGTGGGTAGCCATGCTTCAGAGAAGTCCCAGGCGCCAAAGGCTGCAGTTGTACACGCTGAAGTTAATCATGAACCAGCTCCAGACTTGCTTGACTTGGGTGACTCAACTAGTTCTGCTCCGTCTGTAGATCCTTTTAAGCAGTTAGAAGGGCTTCTTGATCAAACTGACGTTGCGGCAAATGTGAATCATGGTACAGCAAAGACACCTGATTTTATGGGATTATATGCAGATACACCTGTCAGTGGGCTCAGTAGCAGTGTTGGGGATCTTTTGCCGACCAACAGGGATGAGTTTAATCTTACATCTGAGTTATCAAGTGCTACAAGGACTGCTCAAGGTGGGGTAACACAGATTAATAAGGGTCCTAACCCTAAAGATGCCTTGGAAAAGGATTCACGTGTAAGGCAGATGGGCGTGACACCAACAAGGCCGAATCCCAACTTGTTTCGAGATCTGCTTGGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0030117 GO:0030119 GO:0030124 GO:0032991 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0048475 GO:0055044 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04142 map04142

Pfam Domains

Protein Families

Adaptin_N

Protein Analysis

971

Amino Acids

106.8

Weight (kDa)

5.65

Isoelectric Point (pI)

50.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Adaptin_N	PF01602	51 - 595	3e-84	Adaptin N terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001658)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31730
fragaria_vesca	FvH4_5g19200 FvH4_5g25110 FvH4_5g25110 FvH4_5g25110
malus_domestica	MD02G1267100.v1.1 MD07G1052000.v1.1
prunus_persica	Prupe.2G018400_v2.0.a1 Prupe.2G055800_v2.0.a1 Prupe.2G056200_v2.0.a1 Prupe.2G057700_v2.0.a1 Prupe.2G066700_v2.0.a1 Prupe.2G068200_v2.0.a1
pyrus_communis	pycom02g22880 pycom02g22890 pycom02g22910 pycom07g04030 pycom15g15900
rosa_chinensis	RchiOBHm_Chr1g0351661 RchiOBHm_Chr4g0407221 RchiOBHm_Chr7g0215311
rosa_laevigata	RLG00000002671
rosa_multiflora	Rmu_co8017010.1_g000001 Rmu_co8035246.1_g000001 Rmu_co8165622.1_g000001 Rmu_co8288873.1_g000001 Rmu_sc0000355.1_g000018 Rmu_sc0000542.1_g000006 Rmu_sc0004129.1_g000014 Rmu_sc0005117.1_g000009 Rmu_sc0014840.1_g000006 Rmu_sc0015060.1_g000010 Rmu_sc0015060.1_g000011 Rmu_sc0018122.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244290
rosa_rugosa	Rorug06G0151000 Rorug07G0152000
rosa_samantha	Rh1CG270800 Rh2AG294700 Rh7AG288800 Rh7AG297700 Rh7AG297900 Rh7BG280800 Rh7CG308600 Rh7CG317900 Rh7DG292400
rosa_wichuraiana	Rw7G024690 Rw7G025350 Rw7G028510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	687
AasI	GACNNNNNNGTC	1 cut(s)	1613
AatII	GACGTC	1 cut(s)	166
Acc36I	ACCTGC	3 cut(s)	715, 729, 2128
AccB1I	GGYRCC	3 cut(s)	635, 775, 2479
AccB7I	CCANNNNNTGG	1 cut(s)	645
AccBSI	CCGCTC	1 cut(s)	1670
AccI	GTMKAC	3 cut(s)	222, 489, 1051
AclI	AACGTT	1 cut(s)	1145
AclWI	GGATC	8 cut(s)	396, 614, 1206, 1353, 1681, 2158, 2570, 2721
AcoI	YGGCCR	5 cut(s)	22, 58, 238, 647, 2339
AcsI	RAATTY	2 cut(s)	679, 2305
AcuI	CTGAAG	4 cut(s)	1154, 1484, 2448, 2526
AcvI	CACGTG	2 cut(s)	2318, 2846
AcyI	GRCGYC	4 cut(s)	163, 627, 776, 2480
AfaI	GTAC	5 cut(s)	206, 1160, 2184, 2499, 2641
AfeI	AGCGCT	1 cut(s)	1928
AfiI	CCNNNNNNNGG	7 cut(s)	24, 67, 508, 645, 738, 2415, 2690
AflII	CTTAAG	1 cut(s)	2162
AflIII	ACRYGT	1 cut(s)	2845
AgeI	ACCGGT	2 cut(s)	311, 546
AhlI	ACTAGT	1 cut(s)	2555
AjnI	CCWGG	3 cut(s)	45, 480, 2475
AjuI	GAANNNNNNNTTGG	2 cut(s)	2197, 2229
AloI	GAACNNNNNNTCC	2 cut(s)	897, 929
Alw21I	GWGCWC	2 cut(s)	2234, 2240
Alw26I	GTCTC	4 cut(s)	170, 452, 2102, 2143
AlwI	GGATC	8 cut(s)	396, 614, 1206, 1353, 1681, 2158, 2570, 2721
Ama87I	CYCGRG	3 cut(s)	140, 1916, 2233
Aor51HI	AGCGCT	1 cut(s)	1928
AoxI	GGCC	8 cut(s)	22, 58, 238, 647, 750, 2201, 2339, 2876
ApeKI	GCWGC	4 cut(s)	7, 977, 2051, 2489
ApoI	RAATTY	2 cut(s)	679, 2305
ArsI	GACNNNNNNTTYG	2 cut(s)	236, 268
AseI	ATTAAT	2 cut(s)	1227, 2802
AsiGI	ACCGGT	2 cut(s)	311, 546
Asp700I	GAANNNNTTC	2 cut(s)	1549, 2598
AspS9I	GGNCC	2 cut(s)	1117, 2810
AsuC2I	CCSGG	3 cut(s)	26, 624, 1342
AsuHPI	GGTGA	6 cut(s)	536, 839, 1484, 1961, 2217, 2558
AsuII	TTCGAA	1 cut(s)	1332
AvaI	CYCGRG	3 cut(s)	140, 1916, 2233
AvaII	GGWCC	2 cut(s)	1117, 2810
AxyI	CCTNAGG	1 cut(s)	747
BaeGI	GKGCMC	1 cut(s)	2300
BaeI	ACNNNNGTAYC	2 cut(s)	2670, 2703
BanI	GGYRCC	3 cut(s)	635, 775, 2479
BanII	GRGCYC	5 cut(s)	44, 177, 2234, 2240, 2697
BarI	GAAGNNNNNNTAC	2 cut(s)	2446, 2478
BbrPI	CACGTG	2 cut(s)	2318, 2846
BbsI	GAAGAC	2 cut(s)	1321, 1839
Bbv12I	GWGCWC	2 cut(s)	2234, 2240
BbvI	GCAGC	4 cut(s)	19, 964, 2038, 2476
BceAI	ACGGC	6 cut(s)	254, 262, 308, 419, 455, 1082
BcgI	CGANNNNNNTGC	2 cut(s)	2136, 2170
BciT130I	CCWGG	3 cut(s)	47, 482, 2477
BclI	TGATCA	2 cut(s)	357, 2605
BcnI	CCSGG	3 cut(s)	26, 624, 1342
BcoDI	GTCTC	4 cut(s)	170, 452, 2102, 2143
BcuI	ACTAGT	1 cut(s)	2555
BfaI	CTAG	8 cut(s)	584, 1094, 1260, 1349, 1817, 1889, 2087, 2556
BfmI	CTRYAG	4 cut(s)	1419, 2052, 2490, 2571
BfoI	RGCGCY	5 cut(s)	542, 779, 1902, 1930, 2483
BfrI	CTTAAG	1 cut(s)	2162
BfuAI	ACCTGC	3 cut(s)	715, 729, 2128
BglI	GCCNNNNNGGC	2 cut(s)	246, 536
BglII	AGATCT	2 cut(s)	1741, 2899
BlpI	GCTNAGC	1 cut(s)	1902
Bme1390I	CCNGG	6 cut(s)	26, 47, 482, 624, 1342, 2477
Bme18I	GGWCC	2 cut(s)	1117, 2810
BmeT110I	CYCGRG	3 cut(s)	140, 1916, 2233
BmgT120I	GGNCC	2 cut(s)	1117, 2810
BmiI	GGNNCC	7 cut(s)	43, 250, 637, 777, 1119, 2481, 2811
BmrFI	CCNGG	6 cut(s)	26, 47, 482, 624, 1342, 2477
BmrI	ACTGGG	1 cut(s)	1578
BmsI	GCATC	9 cut(s)	138, 589, 1030, 1777, 2132, 2134, 2231, 2450, 2815
BmuI	ACTGGG	1 cut(s)	1578
BpiI	GAAGAC	2 cut(s)	1321, 1839
BpmI	CTGGAG	1 cut(s)	2511
Bpu10I	CCTNAGC	1 cut(s)	1930
Bpu1102I	GCTNAGC	1 cut(s)	1902
Bpu14I	TTCGAA	1 cut(s)	1332
BpuEI	CTTGAG	6 cut(s)	287, 704, 966, 976, 2343, 2766
BpuMI	CCSGG	3 cut(s)	26, 624, 1342
Bsa29I	ATCGAT	1 cut(s)	672
BsaAI	YACGTR	2 cut(s)	2318, 2846
BsaBI	GATNNNNATC	2 cut(s)	618, 762
BsaHI	GRCGYC	4 cut(s)	163, 627, 776, 2480
BsaI	GGTCTC	1 cut(s)	2102
BsaWI	WCCGGW	2 cut(s)	311, 546
BsaXI	ACNNNNNCTCC	2 cut(s)	233, 263
Bsc4I	CCNNNNNNNGG	7 cut(s)	24, 67, 508, 645, 738, 2415, 2690
Bse118I	RCCGGY	2 cut(s)	311, 546
Bse1I	ACTGG	3 cut(s)	1573, 2024, 2081
Bse21I	CCTNAGG	1 cut(s)	747
Bse8I	GATNNNNATC	2 cut(s)	618, 762
BseBI	CCWGG	3 cut(s)	47, 482, 2477
BseCI	ATCGAT	1 cut(s)	672
BseGI	GGATG	4 cut(s)	378, 502, 1414, 2740
BseJI	GATNNNNATC	2 cut(s)	618, 762
BseLI	CCNNNNNNNGG	7 cut(s)	24, 67, 508, 645, 738, 2415, 2690
BseMII	CTCAG	9 cut(s)	352, 406, 1686, 1916, 1944, 2061, 2154, 2710, 2745
BseNI	ACTGG	3 cut(s)	1573, 2024, 2081
BseRI	GAGGAG	7 cut(s)	128, 134, 137, 287, 290, 485, 559
BseSI	GKGCMC	1 cut(s)	2300
BseX3I	CGGCCG	1 cut(s)	22
BseXI	GCAGC	4 cut(s)	19, 964, 2038, 2476
BseYI	CCCAGC	1 cut(s)	2047
Bsh1285I	CGRYCG	1 cut(s)	25
BshFI	GGCC	8 cut(s)	24, 60, 240, 649, 752, 2203, 2341, 2878
BshNI	GGYRCC	3 cut(s)	635, 775, 2479
BshTI	ACCGGT	2 cut(s)	311, 546
BshVI	ATCGAT	1 cut(s)	672
BsiEI	CGRYCG	1 cut(s)	25
BsiHKAI	GWGCWC	2 cut(s)	2234, 2240
BsiHKCI	CYCGRG	3 cut(s)	140, 1916, 2233
BsiSI	CCGG	6 cut(s)	25, 312, 547, 623, 773, 1341
BslFI	GGGAC	1 cut(s)	2458
BslI	CCNNNNNNNGG	7 cut(s)	24, 67, 508, 645, 738, 2415, 2690
BsmAI	GTCTC	4 cut(s)	170, 452, 2102, 2143
BsmBI	CGTCTC	1 cut(s)	170
BsmFI	GGGAC	1 cut(s)	2458
BsmI	GAATGC	1 cut(s)	714
BsnI	GGCC	8 cut(s)	24, 60, 240, 649, 752, 2203, 2341, 2878
Bso31I	GGTCTC	1 cut(s)	2102
BsoBI	CYCGRG	3 cut(s)	140, 1916, 2233
Bsp119I	TTCGAA	1 cut(s)	1332
Bsp1286I	GDGCHC	6 cut(s)	44, 177, 2234, 2240, 2300, 2697
Bsp1407I	TGTACA	3 cut(s)	1158, 2182, 2497
Bsp1720I	GCTNAGC	1 cut(s)	1902
BspANI	GGCC	8 cut(s)	24, 60, 240, 649, 752, 2203, 2341, 2878
BspCNI	CTCAG	9 cut(s)	351, 405, 1687, 1915, 1943, 2062, 2153, 2709, 2746
BspDI	ATCGAT	1 cut(s)	672
BspHI	TCATGA	2 cut(s)	1641, 2515
BspLI	GGNNCC	7 cut(s)	43, 250, 637, 777, 1119, 2481, 2811
BspMAI	CTGCAG	3 cut(s)	1423, 2056, 2494
BspMI	ACCTGC	3 cut(s)	715, 729, 2128
BspPI	GGATC	8 cut(s)	396, 614, 1206, 1353, 1681, 2158, 2570, 2721
BspQI	GCTCTTC	2 cut(s)	875, 2245
BspT104I	TTCGAA	1 cut(s)	1332
BspT107I	GGYRCC	3 cut(s)	635, 775, 2479
BspTI	CTTAAG	1 cut(s)	2162
BspTNI	GGTCTC	1 cut(s)	2102
BsrBI	CCGCTC	1 cut(s)	1670
BsrFI	RCCGGY	2 cut(s)	311, 546
BsrGI	TGTACA	3 cut(s)	1158, 2182, 2497
BsrI	ACTGG	3 cut(s)	1573, 2024, 2081
BssAI	RCCGGY	2 cut(s)	311, 546
BssNI	GRCGYC	4 cut(s)	163, 627, 776, 2480
BssT1I	CCWWGG	4 cut(s)	66, 507, 1045, 2829
Bst2UI	CCWGG	3 cut(s)	47, 482, 2477
Bst4CI	ACNGT	2 cut(s)	463, 1013
Bst6I	CTCTTC	7 cut(s)	338, 875, 998, 2218, 2245, 2386, 2408
BstACI	GRCGYC	4 cut(s)	163, 627, 776, 2480
BstAFI	CTTAAG	1 cut(s)	2162
BstAPI	GCANNNNNTGC	1 cut(s)	933
BstAUI	TGTACA	3 cut(s)	1158, 2182, 2497
BstBAI	YACGTR	2 cut(s)	2318, 2846
BstBI	TTCGAA	1 cut(s)	1332
BstC8I	GCNNGC	7 cut(s)	443, 468, 1275, 1755, 1770, 2201, 2376
BstEII	GGTNACC	1 cut(s)	314
BstF5I	GGATG	4 cut(s)	378, 502, 1414, 2740
BstH2I	RGCGCY	5 cut(s)	542, 779, 1902, 1930, 2483
BstMAI	GTCTC	4 cut(s)	170, 452, 2102, 2143
BstMCI	CGRYCG	1 cut(s)	25
BstNI	CCWGG	3 cut(s)	47, 482, 2477
BstNSI	RCATGY	2 cut(s)	853, 1340
BstPI	GGTNACC	1 cut(s)	314
BstSCI	CCNGG	6 cut(s)	24, 45, 480, 622, 1340, 2475
BstSFI	CTRYAG	4 cut(s)	1419, 2052, 2490, 2571
BstSLI	GKGCMC	1 cut(s)	2300
BstV1I	GCAGC	4 cut(s)	19, 964, 2038, 2476
BstV2I	GAAGAC	2 cut(s)	1321, 1839
BstX2I	RGATCY	7 cut(s)	388, 1345, 1686, 1741, 2575, 2713, 2899
BstYI	RGATCY	7 cut(s)	388, 1345, 1686, 1741, 2575, 2713, 2899
BstZI	CGGCCG	1 cut(s)	22
Bsu15I	ATCGAT	1 cut(s)	672
Bsu36I	CCTNAGG	1 cut(s)	747
BsuRI	GGCC	8 cut(s)	24, 60, 240, 649, 752, 2203, 2341, 2878
BsuTUI	ATCGAT	1 cut(s)	672
BtgZI	GCGATG	1 cut(s)	2112
BtsCI	GGATG	4 cut(s)	378, 502, 1414, 2740
BtsI	GCAGTG	1 cut(s)	2710
BtsIMutI	CAGTG	5 cut(s)	346, 1566, 2088, 2695, 2710
BveI	ACCTGC	3 cut(s)	715, 729, 2128
Cac8I	GCNNGC	7 cut(s)	443, 468, 1275, 1755, 1770, 2201, 2376
CciI	TCATGA	2 cut(s)	1641, 2515
Cfr10I	RCCGGY	2 cut(s)	311, 546
Cfr13I	GGNCC	2 cut(s)	1117, 2810
ClaI	ATCGAT	1 cut(s)	672
CseI	GACGC	2 cut(s)	616, 1829
Csp6I	GTAC	5 cut(s)	205, 1159, 2183, 2498, 2640
CspAI	ACCGGT	2 cut(s)	311, 546
CviQI	GTAC	5 cut(s)	205, 1159, 2183, 2498, 2640
DinI	GGCGCC	2 cut(s)	777, 2481
DrdI	GACNNNNNNGTC	1 cut(s)	1613
DseDI	GACNNNNNNGTC	1 cut(s)	1613
EaeI	YGGCCR	5 cut(s)	22, 58, 238, 647, 2339
EagI	CGGCCG	1 cut(s)	22
Eam1104I	CTCTTC	7 cut(s)	338, 875, 998, 2218, 2245, 2386, 2408
EarI	CTCTTC	7 cut(s)	338, 875, 998, 2218, 2245, 2386, 2408
EciI	GGCGGA	2 cut(s)	131, 203
Ecl136II	GAGCTC	2 cut(s)	2232, 2238
EclXI	CGGCCG	1 cut(s)	22
Eco130I	CCWWGG	4 cut(s)	66, 507, 1045, 2829
Eco147I	AGGCCT	1 cut(s)	752
Eco24I	GRGCYC	5 cut(s)	44, 177, 2234, 2240, 2697
Eco31I	GGTCTC	1 cut(s)	2102
Eco47I	GGWCC	2 cut(s)	1117, 2810
Eco47III	AGCGCT	1 cut(s)	1928
Eco52I	CGGCCG	1 cut(s)	22
Eco53kI	GAGCTC	2 cut(s)	2232, 2238
Eco57I	CTGAAG	4 cut(s)	1154, 1484, 2448, 2526
Eco72I	CACGTG	2 cut(s)	2318, 2846
Eco81I	CCTNAGG	1 cut(s)	747
Eco88I	CYCGRG	3 cut(s)	140, 1916, 2233
Eco91I	GGTNACC	1 cut(s)	314
EcoICRI	GAGCTC	2 cut(s)	2232, 2238
EcoO109I	RGGNCCY	2 cut(s)	1117, 2810
EcoO65I	GGTNACC	1 cut(s)	314
EcoRI	GAATTC	1 cut(s)	679
EcoRII	CCWGG	3 cut(s)	45, 480, 2475
EcoT14I	CCWWGG	4 cut(s)	66, 507, 1045, 2829
EcoT22I	ATGCAT	1 cut(s)	1657
EcoT38I	GRGCYC	5 cut(s)	44, 177, 2234, 2240, 2697
EgeI	GGCGCC	2 cut(s)	777, 2481
EheI	GGCGCC	2 cut(s)	777, 2481
ErhI	CCWWGG	4 cut(s)	66, 507, 1045, 2829
Esp3I	CGTCTC	1 cut(s)	170
FalI	AAGNNNNNCTT	4 cut(s)	2236, 2268, 2586, 2618
FaqI	GGGAC	1 cut(s)	2458
FauI	CCCGC	1 cut(s)	2120
FauNDI	CATATG	1 cut(s)	1759
FbaI	TGATCA	2 cut(s)	357, 2605
FblI	GTMKAC	3 cut(s)	222, 489, 1051
FokI	GGATG	4 cut(s)	365, 489, 1421, 2747
FriOI	GRGCYC	5 cut(s)	44, 177, 2234, 2240, 2697
FspBI	CTAG	8 cut(s)	584, 1094, 1260, 1349, 1817, 1889, 2087, 2556
GsaI	CCCAGC	1 cut(s)	2051
GsuI	CTGGAG	1 cut(s)	2511
HaeII	RGCGCY	5 cut(s)	542, 779, 1902, 1930, 2483
HaeIII	GGCC	8 cut(s)	24, 60, 240, 649, 752, 2203, 2341, 2878
HapII	CCGG	6 cut(s)	25, 312, 547, 623, 773, 1341
HgaI	GACGC	2 cut(s)	616, 1829
Hin1I	GRCGYC	4 cut(s)	163, 627, 776, 2480
HincII	GTYRAC	3 cut(s)	373, 490, 1052
HindII	GTYRAC	3 cut(s)	373, 490, 1052
HindIII	AAGCTT	3 cut(s)	1481, 1977, 2372
HpaII	CCGG	6 cut(s)	25, 312, 547, 623, 773, 1341
HphI	GGTGA	6 cut(s)	536, 839, 1484, 1961, 2217, 2558
Hpy166II	GTNNAC	6 cut(s)	223, 373, 490, 1052, 2183, 2500
Hpy8I	GTNNAC	6 cut(s)	223, 373, 490, 1052, 2183, 2500
Hpy99I	CGWCG	8 cut(s)	165, 248, 290, 416, 458, 491, 1610, 2158
HpyAV	CCTTC	4 cut(s)	193, 262, 379, 2588
HpyCH4III	ACNGT	2 cut(s)	463, 1013
HpyCH4IV	ACGT	6 cut(s)	163, 225, 1145, 2317, 2616, 2845
HpySE526I	ACGT	6 cut(s)	163, 225, 1145, 2317, 2616, 2845
Hsp92I	GRCGYC	4 cut(s)	163, 627, 776, 2480
KasI	GGCGCC	2 cut(s)	775, 2479
Ksp22I	TGATCA	2 cut(s)	357, 2605
LguI	GCTCTTC	2 cut(s)	875, 2245
LmnI	GCTCC	8 cut(s)	4, 47, 124, 254, 1270, 1795, 2530, 2569
Lsp1109I	GCAGC	4 cut(s)	19, 964, 2038, 2476
LweI	GCATC	9 cut(s)	138, 589, 1030, 1777, 2132, 2134, 2231, 2450, 2815
MaeI	CTAG	8 cut(s)	584, 1094, 1260, 1349, 1817, 1889, 2087, 2556
MaeII	ACGT	6 cut(s)	163, 225, 1145, 2317, 2616, 2845
MbiI	CCGCTC	1 cut(s)	1670
MflI	RGATCY	7 cut(s)	388, 1345, 1686, 1741, 2575, 2713, 2899
MhlI	GDGCHC	6 cut(s)	44, 177, 2234, 2240, 2300, 2697
Mly113I	GGCGCC	2 cut(s)	776, 2480
MlyI	GAGTC	4 cut(s)	274, 1460, 1601, 2543
MmeI	TCCRAC	1 cut(s)	2136
Mph1103I	ATGCAT	1 cut(s)	1657
MroXI	GAANNNNTTC	2 cut(s)	1549, 2598
MslI	CAYNNNNRTG	2 cut(s)	674, 854
MspA1I	CMGCKG	2 cut(s)	652, 2051
MspCI	CTTAAG	1 cut(s)	2162
MspI	CCGG	6 cut(s)	25, 312, 547, 623, 773, 1341
MspR9I	CCNGG	6 cut(s)	26, 47, 482, 624, 1342, 2477
Mva1269I	GAATGC	1 cut(s)	714
MvaI	CCWGG	3 cut(s)	47, 482, 2477
NarI	GGCGCC	2 cut(s)	776, 2480
NciI	CCSGG	3 cut(s)	26, 624, 1342
NdeI	CATATG	1 cut(s)	1759
NlaIV	GGNNCC	7 cut(s)	43, 250, 637, 777, 1119, 2481, 2811
NmeAIII	GCCGAG	4 cut(s)	216, 757, 1105, 1417
NmuCI	GTSAC	7 cut(s)	341, 1007, 1013, 2275, 2318, 2546, 2863
NsiI	ATGCAT	1 cut(s)	1657
NspI	RCATGY	2 cut(s)	853, 1340
NspV	TTCGAA	1 cut(s)	1332
PaeR7I	CTCGAG	2 cut(s)	140, 2233
PagI	TCATGA	2 cut(s)	1641, 2515
PasI	CCCWGGG	1 cut(s)	46
PceI	AGGCCT	1 cut(s)	752
PciSI	GCTCTTC	2 cut(s)	875, 2245
PcsI	WCGNNNNNNNCGW	4 cut(s)	138, 216, 624, 1611
PctI	GAATGC	1 cut(s)	714
PdmI	GAANNNNTTC	2 cut(s)	1549, 2598
PfeI	GAWTC	6 cut(s)	887, 1610, 2330, 2632, 2840, 2881
PflFI	GACNNNGTC	2 cut(s)	83, 1171
PflMI	CCANNNNNTGG	1 cut(s)	645
PinAI	ACCGGT	2 cut(s)	311, 546
PleI	GAGTC	4 cut(s)	274, 1459, 1600, 2543
PluTI	GGCGCC	2 cut(s)	779, 2483
PmaCI	CACGTG	2 cut(s)	2318, 2846
PmlI	CACGTG	2 cut(s)	2318, 2846
PpsI	GAGTC	4 cut(s)	274, 1459, 1600, 2543
Ppu21I	YACGTR	2 cut(s)	2318, 2846
PpuMI	RGGWCCY	2 cut(s)	1117, 2810
PshBI	ATTAAT	2 cut(s)	1227, 2802
PsiI	TTATAA	1 cut(s)	687
Psp124BI	GAGCTC	2 cut(s)	2234, 2240
Psp1406I	AACGTT	1 cut(s)	1145
Psp5II	RGGWCCY	2 cut(s)	1117, 2810
Psp6I	CCWGG	3 cut(s)	45, 480, 2475
PspCI	CACGTG	2 cut(s)	2318, 2846
PspEI	GGTNACC	1 cut(s)	314
PspFI	CCCAGC	1 cut(s)	2047
PspGI	CCWGG	3 cut(s)	45, 480, 2475
PspN4I	GGNNCC	7 cut(s)	43, 250, 637, 777, 1119, 2481, 2811
PspPI	GGNCC	2 cut(s)	1117, 2810
PspPPI	RGGWCCY	2 cut(s)	1117, 2810
PspXI	VCTCGAGB	1 cut(s)	2233
PsrI	GAACNNNNNNTAC	2 cut(s)	2151, 2183
PstI	CTGCAG	3 cut(s)	1423, 2056, 2494
PsuI	RGATCY	7 cut(s)	388, 1345, 1686, 1741, 2575, 2713, 2899
PsyI	GACNNNGTC	2 cut(s)	83, 1171
PvuII	CAGCTG	1 cut(s)	2051
RsaI	GTAC	5 cut(s)	206, 1160, 2184, 2499, 2641
RsaNI	GTAC	5 cut(s)	205, 1159, 2183, 2498, 2640
RseI	CAYNNNNRTG	2 cut(s)	674, 854
SacI	GAGCTC	2 cut(s)	2234, 2240
SalI	GTCGAC	2 cut(s)	488, 1050
SapI	GCTCTTC	2 cut(s)	875, 2245
Sau96I	GGNCC	2 cut(s)	1117, 2810
SchI	GAGTC	4 cut(s)	274, 1460, 1601, 2543
ScrFI	CCNGG	6 cut(s)	26, 47, 482, 624, 1342, 2477
SduI	GDGCHC	6 cut(s)	44, 177, 2234, 2240, 2300, 2697
SfaNI	GCATC	9 cut(s)	138, 589, 1030, 1777, 2132, 2134, 2231, 2450, 2815
SfcI	CTRYAG	4 cut(s)	1419, 2052, 2490, 2571
SfoI	GGCGCC	2 cut(s)	777, 2481
Sfr274I	CTCGAG	2 cut(s)	140, 2233
SfuI	TTCGAA	1 cut(s)	1332
SinI	GGWCC	2 cut(s)	1117, 2810
SlaI	CTCGAG	2 cut(s)	140, 2233
SmiMI	CAYNNNNRTG	2 cut(s)	674, 854
SpeI	ACTAGT	1 cut(s)	2555
SseBI	AGGCCT	1 cut(s)	752
SspDI	GGCGCC	2 cut(s)	775, 2479
SspI	AATATT	2 cut(s)	901, 1550
SspMI	CTAG	8 cut(s)	584, 1094, 1260, 1349, 1817, 1889, 2087, 2556
SstI	GAGCTC	2 cut(s)	2234, 2240
StuI	AGGCCT	1 cut(s)	752
StyD4I	CCNGG	6 cut(s)	24, 45, 480, 622, 1340, 2475
StyI	CCWWGG	4 cut(s)	66, 507, 1045, 2829
TaaI	ACNGT	2 cut(s)	463, 1013
TaiI	ACGT	6 cut(s)	166, 228, 1148, 2320, 2619, 2848
TatI	WGTACW	4 cut(s)	204, 1158, 2182, 2497
TauI	GCSGC	7 cut(s)	54, 425, 607, 652, 1816, 2341, 2624
TfiI	GAWTC	6 cut(s)	887, 1610, 2330, 2632, 2840, 2881
TscAI	CASTG	5 cut(s)	346, 1573, 2088, 2695, 2710
TseFI	GTSAC	7 cut(s)	341, 1007, 1013, 2275, 2318, 2546, 2863
TseI	GCWGC	4 cut(s)	7, 977, 2051, 2489
Tsp45I	GTSAC	7 cut(s)	341, 1007, 1013, 2275, 2318, 2546, 2863
TspDTI	ATGAA	8 cut(s)	212, 1334, 1394, 1658, 1776, 1848, 1989, 2532
TspGWI	ACGGA	2 cut(s)	1603, 2556
TspRI	CASTG	5 cut(s)	346, 1573, 2088, 2695, 2710
Tth111I	GACNNNGTC	2 cut(s)	83, 1171
Van91I	CCANNNNNTGG	1 cut(s)	645
Vha464I	CTTAAG	1 cut(s)	2162
VpaK11BI	GGWCC	2 cut(s)	1117, 2810
VspI	ATTAAT	2 cut(s)	1227, 2802
XapI	RAATTY	2 cut(s)	679, 2305
XbaI	TCTAGA	1 cut(s)	1259
XceI	RCATGY	2 cut(s)	853, 1340
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1299
XhoI	CTCGAG	2 cut(s)	140, 2233
XmiI	GTMKAC	3 cut(s)	222, 489, 1051
XmnI	GAANNNNTTC	2 cut(s)	1549, 2598
XspI	CTAG	8 cut(s)	584, 1094, 1260, 1349, 1817, 1889, 2087, 2556
ZraI	GACGTC	1 cut(s)	164
Zsp2I	ATGCAT	1 cut(s)	1657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.