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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 13 |
| AclWI | GGATC | 2 cut(s) | 48, 61 |
| AfaI | GTAC | 1 cut(s) | 29 |
| AgsI | TTSAA | 2 cut(s) | 133, 209 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 99, 131 |
| AluBI | AGCT | 1 cut(s) | 10 |
| AluI | AGCT | 1 cut(s) | 10 |
| Alw21I | GWGCWC | 1 cut(s) | 12 |
| AlwI | GGATC | 2 cut(s) | 48, 61 |
| AsuHPI | GGTGA | 2 cut(s) | 10, 182 |
| BamHI | GGATCC | 1 cut(s) | 53 |
| BanII | GRGCYC | 1 cut(s) | 12 |
| Bbv12I | GWGCWC | 1 cut(s) | 12 |
| BfaI | CTAG | 1 cut(s) | 222 |
| BmiI | GGNNCC | 1 cut(s) | 55 |
| BseYI | CCCAGC | 1 cut(s) | 252 |
| BsiHKAI | GWGCWC | 1 cut(s) | 12 |
| Bsp1286I | GDGCHC | 1 cut(s) | 12 |
| Bsp143I | GATC | 3 cut(s) | 53, 172, 224 |
| BspACI | CCGC | 1 cut(s) | 13 |
| BspLI | GGNNCC | 1 cut(s) | 55 |
| BspPI | GGATC | 2 cut(s) | 48, 61 |
| BssMI | GATC | 3 cut(s) | 53, 172, 224 |
| Bst4CI | ACNGT | 1 cut(s) | 104 |
| BstC8I | GCNNGC | 1 cut(s) | 8 |
| BstKTI | GATC | 3 cut(s) | 56, 175, 227 |
| BstMBI | GATC | 3 cut(s) | 53, 172, 224 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 12 |
| BstX2I | RGATCY | 1 cut(s) | 53 |
| BstYI | RGATCY | 1 cut(s) | 53 |
| BtsIMutI | CAGTG | 1 cut(s) | 100 |
| Cac8I | GCNNGC | 1 cut(s) | 8 |
| CseI | GACGC | 1 cut(s) | 151 |
| Csp6I | GTAC | 1 cut(s) | 28 |
| CviJI | RGCY | 3 cut(s) | 10, 92, 120 |
| CviKI_1 | RGCY | 3 cut(s) | 10, 92, 120 |
| CviQI | GTAC | 1 cut(s) | 28 |
| DpnI | GATC | 3 cut(s) | 55, 174, 226 |
| DpnII | GATC | 3 cut(s) | 53, 172, 224 |
| Ecl136II | GAGCTC | 1 cut(s) | 10 |
| Eco24I | GRGCYC | 1 cut(s) | 12 |
| Eco53kI | GAGCTC | 1 cut(s) | 10 |
| EcoICRI | GAGCTC | 1 cut(s) | 10 |
| EcoT38I | GRGCYC | 1 cut(s) | 12 |
| FaiI | YATR | 1 cut(s) | 41 |
| FriOI | GRGCYC | 1 cut(s) | 12 |
| FspBI | CTAG | 1 cut(s) | 222 |
| GsaI | CCCAGC | 1 cut(s) | 256 |
| HgaI | GACGC | 1 cut(s) | 151 |
| HphI | GGTGA | 2 cut(s) | 10, 182 |
| Hpy188I | TCNGA | 2 cut(s) | 58, 262 |
| Hpy188III | TCNNGA | 1 cut(s) | 149 |
| Hpy99I | CGWCG | 1 cut(s) | 28 |
| HpyCH4III | ACNGT | 1 cut(s) | 104 |
| HpyCH4IV | ACGT | 1 cut(s) | 26 |
| HpyCH4V | TGCA | 1 cut(s) | 100 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 12 |
| HpySE526I | ACGT | 1 cut(s) | 26 |
| Kzo9I | GATC | 3 cut(s) | 53, 172, 224 |
| LmnI | GCTCC | 1 cut(s) | 15 |
| LpnPI | CCDG | 4 cut(s) | 74, 134, 238, 244 |
| MaeI | CTAG | 1 cut(s) | 222 |
| MaeII | ACGT | 1 cut(s) | 26 |
| MalI | GATC | 3 cut(s) | 55, 174, 226 |
| MboI | GATC | 3 cut(s) | 53, 172, 224 |
| MboII | GAAGA | 2 cut(s) | 221, 253 |
| MfeI | CAATTG | 1 cut(s) | 95 |
| MflI | RGATCY | 1 cut(s) | 53 |
| MhlI | GDGCHC | 1 cut(s) | 12 |
| MluCI | AATT | 6 cut(s) | 31, 48, 76, 95, 136, 154 |
| MmeI | TCCRAC | 1 cut(s) | 81 |
| MnlI | CCTC | 2 cut(s) | 26, 71 |
| MseI | TTAA | 1 cut(s) | 122 |
| MunI | CAATTG | 1 cut(s) | 95 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 12 |
| NdeII | GATC | 3 cut(s) | 53, 172, 224 |
| NlaIV | GGNNCC | 1 cut(s) | 55 |
| Psp124BI | GAGCTC | 1 cut(s) | 12 |
| PspFI | CCCAGC | 1 cut(s) | 252 |
| PspN4I | GGNNCC | 1 cut(s) | 55 |
| PsuI | RGATCY | 1 cut(s) | 53 |
| RsaI | GTAC | 1 cut(s) | 29 |
| RsaNI | GTAC | 1 cut(s) | 28 |
| SacI | GAGCTC | 1 cut(s) | 12 |
| SaqAI | TTAA | 1 cut(s) | 122 |
| Sau3AI | GATC | 3 cut(s) | 53, 172, 224 |
| SduI | GDGCHC | 1 cut(s) | 12 |
| SetI | ASST | 4 cut(s) | 12, 29, 63, 172 |
| Sse9I | AATT | 6 cut(s) | 31, 48, 76, 95, 136, 154 |
| SsiI | CCGC | 1 cut(s) | 13 |
| SspMI | CTAG | 1 cut(s) | 222 |
| SstI | GAGCTC | 1 cut(s) | 12 |
| TaaI | ACNGT | 1 cut(s) | 104 |
| TaiI | ACGT | 1 cut(s) | 29 |
| TasI | AATT | 6 cut(s) | 31, 48, 76, 95, 136, 154 |
| Tru1I | TTAA | 1 cut(s) | 122 |
| Tru9I | TTAA | 1 cut(s) | 122 |
| TscAI | CASTG | 1 cut(s) | 107 |
| TspRI | CASTG | 1 cut(s) | 107 |
| XspI | CTAG | 1 cut(s) | 222 |