Prupe.2G056200_v2.0.a1

AP-4 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001658
Physical Location & Seq
Reverse (-)
6685012 .. 6695117
10106 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G056200.1

Sequence Viewer

Length: 2925 bp
ATGGAGCAACTGAAGACCATAGGGCGAGAGCTTGCGATGGGTTCTCAAGGCGGCTTCGGCCAATCCAAGGAGTTCCTGGACCTCGTCAAGTCTATCGGCGAAGCTCGATCCAAGGCCGAAGAAGAGCGCATCGTCCTCCTCGAGATCGAAACCCTAAAACGACGTCTCTCCGAGCCTGAAATCCCCAAGCGCAAGATGAAGGAGTACATCATCCGCCTCGTCTACGTCGAAATGCTCGGCCACGACGGCTCCTTCGCCTACATCCACGCCGTCAAGATGACCCACGACGACAATCTCTTGCTTAAACGCACCGGTTACCTCGCCGTTTCGCTCTTCCTCAGCGACGATCACGATTTGATCATCTTGATCGTCAATACCATCCAGAAGGATCTCAAGTCTGACAATTACCTTGTCGTTTGTGCCGCCCTCAATGCAGTTTGCAAGCTCATCAACGACGAGACTGTGCCTGCCGTTTTGCCCCAGGTCGTGGACCTCCTCGCCCATCCTAAGGAGGCTGTCAGGAAGAAGGCCATCATGGCGCTTCACCGCTTTTATCAGAAATCGCCATCTTCTGTTTCGCATCTGGTCTCCAATTTCAGGAAGCGCCTTTGTGATAACGATCCGGGAGTCATGGGTGCCACGCTCTGCCCGTTGTTTGACCTCATTACAATCGATGTGAATTCTTATAAGGATTTGGTGGTCAGCTTTGTCAGCATTCTCAAACAGGTCGCCGAGCGCAGGTTGCCCAAGACCTACGATTACCATCAGTTGCCGGCGCCCTTTATTCAGATTAGATTGTTGAAGATCTTGGCATTGTTGGGGAATGGTGATAAGCAATCAAGTGAGAAGATGTATATGGTAGTGGGCGATATTTTTAGGAAGTGCGATTCTTCCAGTAATATAGGAAATGCTGTACTTTATGAGTGTATTTGTTGCGTTTCTGCCATATATCCAAATCCTAAGTTGTTAGAACAGGCTGCTCAAGTCATCTCAAGATTTTTGAAGAGTGACAGTCATAATTTGAAATATATGGGCATCGATGCCCTTGGTCGACTAATAAAGATTAGTCCGGAGATAGCAGAGCAACATCAACTAGCCGTCATTGATTGCTTAGAGGACCCAGATGATACTCTGAAGCGAAAAACATTTGAACTATTGTACAAGATGACCAAGTCCTCCAATGTTGAAGTGATTGTGGATCGTATGATTGATTACATGATTAGCATTAATGATAACCATTACAAAACATATATAGCTTCTAGATGTGTGGAGCTTGCGGAGCAATTTGCACCAAGTAATCAATGGTTTATCCAGACCATGAATAAAGTGTTTGAACATGCGGGAGATTTGGTAAATGTCAAGGTGGCACATAACTTGATGAAGTTGATTGCTGAGGGATTTGGAGAGGATGATGATTCTGCAGATAGTCAGTTGAGATCATCTGCGGTGGAGTCATATTTGCGTATACTTGGTGAGCCAAAGCTTCCATCTGTATTTCTTCAGGTCATTTGTTGGGTTTTGGGGGAATATGGAACTGCTGATGGAAAATATTCTGCTTCCTATATCACTGGGAAGTTATGTGATGTGGCAGAGGCGTATTCAAATGATGAATCCGTCAAGGCTTATGCAGTTACAGCAATCATGAAAATATATGCCTTTGAAATTTCAGCTCAGAGAAAAGTGGATATCCTACCTGAGTGTCAATCTTTGGTGGAAGAATTGTCAGCTTCTCACTCAACAGATCTGCAGCAGCGTGCATATGAATTGCAAGCTGTCATTAGCTTAGATGCTCCTGCAGTTGAGAGTATTATGCCGTCAGACGCTAGTTGTGAAGACATTGAGATTGATAAAAGCCTTTCATTCCTCAATGGCTACGTCCAGCAAGCACTAGAGAAAGGTGCTCAGCCCTATATTCCCGAGAATGAGCGCTCTGGAATGTTAAATATCAGCAACTTCAGTAACCAAGATCAGCATGAAGCTTTAACCCATGGTCTTAGGTTTGAGGCATATGAGCTTCCAAAGCTAGCTGTGCCATCAAGAATTCCTCCAGCTGCAGTTGCATCCTCAACCGAGCTTGTTCCAGTTCCTGAACCATCTTATGCTAGGGAGATCCGCCAGCCTGCATCATTGCCACCTGTGTCAGATTCAGGATCATCGGAACTTAAGTTACGACTAGATGGTGTTCAAAGAAAATGGGGTAGGCCGACATATTCCACCCCAGCATTGTCGATCTCAAATTCCTCTAGTTCTAGTTCCCAGAAATCAGCAAATGGGGTCACACAAGTAGATAGCGTGAGCACTTCAAATTCAAAAGCACGTGATACTTATGAGTCAAGGCGGCCCCAGGTTGAAATTTCTCCAGAAAAGCAGAAGCTTGCCAGTTCACTGTTTGGGGGATCATCAAAAACCGAGAGGAGGCAGTCTTCTGCCAACCATAAGGTGTCTAAGGCGAATATCCATGCTTCAGAGAAGCCCCAGGTACCAAAGGCAGCAGCTGTACACACTGAAGTTAATCATGAACCTGCTCCAGACTTGCTCGATTTGGGTGACTCAACTAGTAGTACTGCTCCCACTGTAGATCCTTTCAAGCAGTTAGAAGGGCTTCTTGATCAAACTGAAGTTGCATTAACTGCAAATCATGGTGCAGCTGATGCTGCTAAGACACCTGATGTTATGGGATTATATGCAGATACATCTCTCAGCAGGCTCAGTAGCAGTGTTGATGACCCTTTGCCGACCAATAGGGATGAGTTTAATCTTGCATCTGAGTTATCAAATGCTACAAGAACTGCTCAAAGTGGGGTAACACAATTGAATAAGGGTCCTAACCCTAAAGATTCCTTGGAAAAGGATGCACTTGTAAGGCAGATGGGTGTGACCCCAACAAGTCAGAATCCCAACTTGTTTAAAGATTTGCTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
Adaptin_N

Protein Analysis

975

Amino Acids

107.46

Weight (kDa)

5.59

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AatII GACGTC 1 cut(s) 166
Acc36I ACCTGC 2 cut(s) 729, 2530
Acc65I GGTACC 1 cut(s) 2479
AccB1I GGYRCC 3 cut(s) 635, 775, 2479
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 3 cut(s) 222, 1051, 1465
AccIII TCCGGA 1 cut(s) 1069
AciI CCGC 9 cut(s) 51, 214, 423, 547, 1277, 1340, 1445, 2113, 2338
AclWI GGATC 8 cut(s) 102, 396, 614, 1206, 2104, 2158, 2404, 2573
AcoI YGGCCR 2 cut(s) 58, 238
AcsI RAATTY 6 cut(s) 679, 1662, 2040, 2236, 2305, 2352
AcuI CTGAAG 7 cut(s) 32, 1154, 1484, 1939, 2448, 2526, 2637
AcvI CACGTG 1 cut(s) 2318
AcyI GRCGYC 2 cut(s) 163, 776
AfaI GTAC 6 cut(s) 206, 915, 1160, 2481, 2499, 2563
AfeI AGCGCT 1 cut(s) 1928
AfiI CCNNNNNNNGG 6 cut(s) 67, 487, 508, 597, 738, 2415
AflII CTTAAG 1 cut(s) 2162
AgeI ACCGGT 1 cut(s) 311
AhlI ACTAGT 1 cut(s) 2555
AjnI CCWGG 4 cut(s) 75, 480, 2343, 2475
Alw21I GWGCWC 2 cut(s) 1903, 2300
Alw26I GTCTC 3 cut(s) 170, 452, 592
AlwI GGATC 8 cut(s) 102, 396, 614, 1206, 2104, 2158, 2404, 2573
AlwNI CAGNNNCTG 2 cut(s) 2087, 2495
Ama87I CYCGRG 2 cut(s) 140, 1916
Aor13HI TCCGGA 1 cut(s) 1069
Aor51HI AGCGCT 1 cut(s) 1928
AoxI GGCC 6 cut(s) 58, 114, 238, 528, 2201, 2339
ApeKI GCWGC 8 cut(s) 977, 1747, 1750, 2051, 2489, 2492, 2645, 2654
ApoI RAATTY 6 cut(s) 679, 1662, 2040, 2236, 2305, 2352
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AseI ATTAAT 1 cut(s) 1227
AsiGI ACCGGT 1 cut(s) 311
Asp700I GAANNNNTTC 2 cut(s) 1549, 2601
Asp718I GGTACC 1 cut(s) 2479
AspLEI GCGC 7 cut(s) 129, 192, 541, 606, 738, 778, 1929
AspS9I GGNCC 5 cut(s) 79, 490, 1117, 2340, 2822
AsuC2I CCSGG 1 cut(s) 624
AsuHPI GGTGA 4 cut(s) 536, 839, 1484, 2558
AsuNHI GCTAGC 1 cut(s) 2023
AvaI CYCGRG 2 cut(s) 140, 1916
AvaII GGWCC 4 cut(s) 79, 490, 1117, 2822
AxyI CCTNAGG 1 cut(s) 507
BanI GGYRCC 3 cut(s) 635, 775, 2479
BbrPI CACGTG 1 cut(s) 2318
BbsI GAAGAC 3 cut(s) 20, 1839, 2415
Bbv12I GWGCWC 2 cut(s) 1903, 2300
BbvCI CCTCAGC 2 cut(s) 338, 1392
BbvI GCAGC 8 cut(s) 964, 1759, 1762, 2038, 2501, 2504, 2641, 2657
BceAI ACGGC 6 cut(s) 254, 262, 308, 455, 1082, 1798
BciT130I CCWGG 4 cut(s) 77, 482, 2345, 2477
BclI TGATCA 2 cut(s) 357, 2608
BcnI CCSGG 1 cut(s) 624
BcoDI GTCTC 3 cut(s) 170, 452, 592
BcuI ACTAGT 1 cut(s) 2555
BfmI CTRYAG 5 cut(s) 1419, 1745, 1794, 2052, 2574
BfoI RGCGCY 4 cut(s) 542, 607, 779, 1930
BfrI CTTAAG 1 cut(s) 2162
BfuAI ACCTGC 2 cut(s) 729, 2530
BglI GCCNNNNNGGC 2 cut(s) 246, 536
BglII AGATCT 2 cut(s) 804, 1741
BlpI GCTNAGC 1 cut(s) 1902
BmcAI AGTACT 1 cut(s) 2563
Bme1390I CCNGG 5 cut(s) 77, 482, 624, 2345, 2477
Bme18I GGWCC 4 cut(s) 79, 490, 1117, 2822
BmeT110I CYCGRG 2 cut(s) 140, 1916
BmgT120I GGNCC 5 cut(s) 79, 490, 1117, 2340, 2822
BmiI GGNNCC 7 cut(s) 250, 637, 777, 1119, 2342, 2481, 2823
BmrFI CCNGG 5 cut(s) 77, 482, 624, 2345, 2477
BmrI ACTGGG 1 cut(s) 1578
BmtI GCTAGC 1 cut(s) 2027
BmuI ACTGGG 1 cut(s) 1578
BpiI GAAGAC 3 cut(s) 20, 1839, 2415
BpmI CTGGAG 3 cut(s) 2031, 2343, 2511
Bpu10I CCTNAGC 2 cut(s) 338, 1392
Bpu1102I GCTNAGC 1 cut(s) 1902
BpuEI CTTGAG 4 cut(s) 30, 377, 966, 976
BpuMI CCSGG 1 cut(s) 624
Bsa29I ATCGAT 2 cut(s) 672, 1038
BsaAI YACGTR 1 cut(s) 2318
BsaBI GATNNNNATC 2 cut(s) 618, 762
BsaHI GRCGYC 2 cut(s) 163, 776
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 8 cut(s) 66, 111, 480, 1045, 1987, 2343, 2475, 2841
BsaWI WCCGGW 2 cut(s) 311, 1069
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 1335, 1365
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 487, 508, 597, 738, 2415
Bse118I RCCGGY 2 cut(s) 311, 772
Bse1I ACTGG 4 cut(s) 894, 1573, 2081, 2379
Bse21I CCTNAGG 1 cut(s) 507
Bse3DI GCAATG 1 cut(s) 2126
Bse8I GATNNNNATC 2 cut(s) 618, 762
BseAI TCCGGA 1 cut(s) 1069
BseBI CCWGG 4 cut(s) 77, 482, 2345, 2477
BseCI ATCGAT 2 cut(s) 672, 1038
BseDI CCNNGG 8 cut(s) 66, 111, 480, 1045, 1987, 2343, 2475, 2841
BseGI GGATG 8 cut(s) 210, 261, 378, 502, 1414, 2060, 2752, 2857
BseJI GATNNNNATC 2 cut(s) 618, 762
BseLI CCNNNNNNNGG 6 cut(s) 67, 487, 508, 597, 738, 2415
BseMI GCAATG 1 cut(s) 2126
BseMII CTCAG 8 cut(s) 352, 1383, 1685, 1686, 1916, 2713, 2722, 2757
BseNI ACTGG 4 cut(s) 894, 1573, 2081, 2379
BseRI GAGGAG 3 cut(s) 128, 485, 2428
BseXI GCAGC 8 cut(s) 964, 1759, 1762, 2038, 2501, 2504, 2641, 2657
BseYI CCCAGC 1 cut(s) 2218
BsgI GTGCAG 1 cut(s) 2664
BshFI GGCC 6 cut(s) 60, 116, 240, 530, 2203, 2341
BshNI GGYRCC 3 cut(s) 635, 775, 2479
BshTI ACCGGT 1 cut(s) 311
BshVI ATCGAT 2 cut(s) 672, 1038
BsiHKAI GWGCWC 2 cut(s) 1903, 2300
BsiHKCI CYCGRG 2 cut(s) 140, 1916
BsiSI CCGG 4 cut(s) 312, 623, 773, 1070
BslI CCNNNNNNNGG 6 cut(s) 67, 487, 508, 597, 738, 2415
BsmAI GTCTC 3 cut(s) 170, 452, 592
BsmBI CGTCTC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 6 cut(s) 60, 116, 240, 530, 2203, 2341
Bso31I GGTCTC 1 cut(s) 592
BsoBI CYCGRG 2 cut(s) 140, 1916
Bsp1286I GDGCHC 2 cut(s) 1903, 2300
Bsp13I TCCGGA 1 cut(s) 1069
Bsp1407I TGTACA 2 cut(s) 1158, 2497
Bsp1720I GCTNAGC 1 cut(s) 1902
Bsp19I CCATGG 1 cut(s) 1987
BspACI CCGC 9 cut(s) 51, 214, 423, 547, 1277, 1340, 1445, 2113, 2338
BspANI GGCC 6 cut(s) 60, 116, 240, 530, 2203, 2341
BspCNI CTCAG 8 cut(s) 351, 1384, 1684, 1687, 1915, 2712, 2721, 2758
BspDI ATCGAT 2 cut(s) 672, 1038
BspEI TCCGGA 1 cut(s) 1069
BspHI TCATGA 2 cut(s) 1641, 2515
BspLI GGNNCC 7 cut(s) 250, 637, 777, 1119, 2342, 2481, 2823
BspMAI CTGCAG 4 cut(s) 1423, 1749, 1798, 2056
BspMI ACCTGC 2 cut(s) 729, 2530
BspOI GCTAGC 1 cut(s) 2027
BspPI GGATC 8 cut(s) 102, 396, 614, 1206, 2104, 2158, 2404, 2573
BspQI GCTCTTC 2 cut(s) 117, 338
BspT107I GGYRCC 3 cut(s) 635, 775, 2479
BspTI CTTAAG 1 cut(s) 2162
BspTNI GGTCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 2126
BsrFI RCCGGY 2 cut(s) 311, 772
BsrGI TGTACA 2 cut(s) 1158, 2497
BsrI ACTGG 4 cut(s) 894, 1573, 2081, 2379
BssAI RCCGGY 2 cut(s) 311, 772
BssECI CCNNGG 8 cut(s) 66, 111, 480, 1045, 1987, 2343, 2475, 2841
BssNAI GTATAC 1 cut(s) 1466
BssNI GRCGYC 2 cut(s) 163, 776
BssT1I CCWWGG 5 cut(s) 66, 111, 1045, 1987, 2841
Bst1107I GTATAC 1 cut(s) 1466
Bst2UI CCWGG 4 cut(s) 77, 482, 2345, 2477
Bst4CI ACNGT 4 cut(s) 463, 1013, 2388, 2575
Bst6I CTCTTC 3 cut(s) 117, 338, 998
BstACI GRCGYC 2 cut(s) 163, 776
BstAFI CTTAAG 1 cut(s) 2162
BstAPI GCANNNNNTGC 2 cut(s) 2630, 2651
BstAUI TGTACA 2 cut(s) 1158, 2497
BstBAI YACGTR 1 cut(s) 2318
BstDSI CCRYGG 1 cut(s) 1987
BstEII GGTNACC 1 cut(s) 314
BstF5I GGATG 8 cut(s) 210, 261, 378, 502, 1414, 2060, 2752, 2857
BstH2I RGCGCY 4 cut(s) 542, 607, 779, 1930
BstHHI GCGC 7 cut(s) 129, 192, 541, 606, 738, 778, 1929
BstMAI GTCTC 3 cut(s) 170, 452, 592
BstNI CCWGG 4 cut(s) 77, 482, 2345, 2477
BstNSI RCATGY 1 cut(s) 1340
BstPI GGTNACC 1 cut(s) 314
BstSCI CCNGG 5 cut(s) 75, 480, 622, 2343, 2475
BstSFI CTRYAG 5 cut(s) 1419, 1745, 1794, 2052, 2574
BstV1I GCAGC 8 cut(s) 964, 1759, 1762, 2038, 2501, 2504, 2641, 2657
BstV2I GAAGAC 3 cut(s) 20, 1839, 2415
BstX2I RGATCY 5 cut(s) 388, 804, 1741, 2109, 2578
BstYI RGATCY 5 cut(s) 388, 804, 1741, 2109, 2578
BstZ17I GTATAC 1 cut(s) 1466
Bsu15I ATCGAT 2 cut(s) 672, 1038
Bsu36I CCTNAGG 1 cut(s) 507
BsuRI GGCC 6 cut(s) 60, 116, 240, 530, 2203, 2341
BsuTUI ATCGAT 2 cut(s) 672, 1038
BtgI CCRYGG 1 cut(s) 1987
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 8 cut(s) 210, 261, 378, 502, 1414, 2060, 2752, 2857
BtsI GCAGTG 1 cut(s) 2722
BtsIMutI CAGTG 5 cut(s) 1566, 2384, 2502, 2571, 2722
BveI ACCTGC 2 cut(s) 729, 2530
CaiI CAGNNNCTG 2 cut(s) 2087, 2495
CciI TCATGA 2 cut(s) 1641, 2515
CfoI GCGC 7 cut(s) 129, 192, 541, 606, 738, 778, 1929
Cfr10I RCCGGY 2 cut(s) 311, 772
Cfr13I GGNCC 5 cut(s) 79, 490, 1117, 2340, 2822
ClaI ATCGAT 2 cut(s) 672, 1038
CseI GACGC 1 cut(s) 1829
Csp6I GTAC 6 cut(s) 205, 914, 1159, 2480, 2498, 2562
CspAI ACCGGT 1 cut(s) 311
CviQI GTAC 6 cut(s) 205, 914, 1159, 2480, 2498, 2562
DinI GGCGCC 1 cut(s) 777
DraI TTTAAA 1 cut(s) 2908
EaeI YGGCCR 2 cut(s) 58, 238
Eam1104I CTCTTC 3 cut(s) 117, 338, 998
EarI CTCTTC 3 cut(s) 117, 338, 998
EciI GGCGGA 2 cut(s) 203, 2102
Eco130I CCWWGG 5 cut(s) 66, 111, 1045, 1987, 2841
Eco31I GGTCTC 1 cut(s) 592
Eco32I GATATC 1 cut(s) 1687
Eco47I GGWCC 4 cut(s) 79, 490, 1117, 2822
Eco47III AGCGCT 1 cut(s) 1928
Eco57I CTGAAG 7 cut(s) 32, 1154, 1484, 1939, 2448, 2526, 2637
Eco72I CACGTG 1 cut(s) 2318
Eco81I CCTNAGG 1 cut(s) 507
Eco88I CYCGRG 2 cut(s) 140, 1916
Eco91I GGTNACC 1 cut(s) 314
EcoO109I RGGNCCY 2 cut(s) 1117, 2822
EcoO65I GGTNACC 1 cut(s) 314
EcoRI GAATTC 2 cut(s) 679, 2040
EcoRII CCWGG 4 cut(s) 75, 480, 2343, 2475
EcoRV GATATC 1 cut(s) 1687
EcoT14I CCWWGG 5 cut(s) 66, 111, 1045, 1987, 2841
EgeI GGCGCC 1 cut(s) 777
EheI GGCGCC 1 cut(s) 777
ErhI CCWWGG 5 cut(s) 66, 111, 1045, 1987, 2841
Esp3I CGTCTC 1 cut(s) 170
FalI AAGNNNNNCTT 3 cut(s) 2589, 2621, 2901
FauI CCCGC 1 cut(s) 1333
FauNDI CATATG 2 cut(s) 1759, 2008
FbaI TGATCA 2 cut(s) 357, 2608
FblI GTMKAC 3 cut(s) 222, 1051, 1465
FokI GGATG 8 cut(s) 197, 248, 365, 489, 1421, 2047, 2759, 2864
GlaI GCGC 7 cut(s) 128, 191, 540, 605, 737, 777, 1928
GsaI CCCAGC 1 cut(s) 2222
GsuI CTGGAG 3 cut(s) 2031, 2343, 2511
HaeII RGCGCY 4 cut(s) 542, 607, 779, 1930
HaeIII GGCC 6 cut(s) 60, 116, 240, 530, 2203, 2341
HapII CCGG 4 cut(s) 312, 623, 773, 1070
HgaI GACGC 1 cut(s) 1829
HhaI GCGC 7 cut(s) 129, 192, 541, 606, 738, 778, 1929
Hin1I GRCGYC 2 cut(s) 163, 776
Hin6I GCGC 7 cut(s) 127, 190, 539, 604, 736, 776, 1927
HinP1I GCGC 7 cut(s) 127, 190, 539, 604, 736, 776, 1927
HincII GTYRAC 1 cut(s) 1052
HindII GTYRAC 1 cut(s) 1052
HindIII AAGCTT 3 cut(s) 1481, 1977, 2372
HpaII CCGG 4 cut(s) 312, 623, 773, 1070
HphI GGTGA 4 cut(s) 536, 839, 1484, 2558
Hpy166II GTNNAC 6 cut(s) 223, 490, 1052, 1466, 2384, 2500
Hpy8I GTNNAC 6 cut(s) 223, 490, 1052, 1466, 2384, 2500
Hpy99I CGWCG 6 cut(s) 165, 230, 248, 290, 347, 458
HpyAV CCTTC 5 cut(s) 193, 262, 379, 520, 2591
HpyCH4III ACNGT 4 cut(s) 463, 1013, 2388, 2575
HpyCH4IV ACGT 4 cut(s) 163, 225, 1875, 2317
HpySE526I ACGT 4 cut(s) 163, 225, 1875, 2317
Hsp92I GRCGYC 2 cut(s) 163, 776
HspAI GCGC 7 cut(s) 127, 190, 539, 604, 736, 776, 1927
KasI GGCGCC 1 cut(s) 775
Kpn2I TCCGGA 1 cut(s) 1069
KpnI GGTACC 1 cut(s) 2483
KroI GCCGGC 1 cut(s) 772
KroNI GCCGGC 1 cut(s) 774
Ksp22I TGATCA 2 cut(s) 357, 2608
LguI GCTCTTC 2 cut(s) 117, 338
LmnI GCTCC 7 cut(s) 4, 254, 1270, 1279, 1795, 2530, 2572
Lsp1109I GCAGC 8 cut(s) 964, 1759, 1762, 2038, 2501, 2504, 2641, 2657
MaeII ACGT 4 cut(s) 163, 225, 1875, 2317
MaeIII GTNAC 9 cut(s) 314, 1007, 1630, 1958, 2166, 2275, 2546, 2803, 2875
MfeI CAATTG 1 cut(s) 2810
MflI RGATCY 5 cut(s) 388, 804, 1741, 2109, 2578
MhlI GDGCHC 2 cut(s) 1903, 2300
Mly113I GGCGCC 1 cut(s) 776
MlyI GAGTC 4 cut(s) 636, 1460, 2339, 2543
MroI TCCGGA 1 cut(s) 1069
MroNI GCCGGC 1 cut(s) 772
MroXI GAANNNNTTC 2 cut(s) 1549, 2601
MseI TTAA 9 cut(s) 303, 1227, 1940, 1982, 2163, 2511, 2627, 2754, 2907
MspA1I CMGCKG 3 cut(s) 2051, 2495, 2648
MspCI CTTAAG 1 cut(s) 2162
MspI CCGG 4 cut(s) 312, 623, 773, 1070
MspR9I CCNGG 5 cut(s) 77, 482, 624, 2345, 2477
MunI CAATTG 1 cut(s) 2810
Mva1269I GAATGC 1 cut(s) 714
MvaI CCWGG 4 cut(s) 77, 482, 2345, 2477
NaeI GCCGGC 1 cut(s) 774
NarI GGCGCC 1 cut(s) 776
NciI CCSGG 1 cut(s) 624
NcoI CCATGG 1 cut(s) 1987
NdeI CATATG 2 cut(s) 1759, 2008
NgoMIV GCCGGC 1 cut(s) 772
NheI GCTAGC 1 cut(s) 2023
NlaIV GGNNCC 7 cut(s) 250, 637, 777, 1119, 2342, 2481, 2823
NmeAIII GCCGAG 2 cut(s) 216, 757
NmuCI GTSAC 4 cut(s) 1007, 2275, 2546, 2875
NspI RCATGY 1 cut(s) 1340
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 2 cut(s) 1641, 2515
PciSI GCTCTTC 2 cut(s) 117, 338
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 225, 647
PctI GAATGC 1 cut(s) 714
PdiI GCCGGC 1 cut(s) 774
PdmI GAANNNNTTC 2 cut(s) 1549, 2601
PfeI GAWTC 6 cut(s) 887, 1415, 1610, 2144, 2837, 2893
PflFI GACNNNGTC 2 cut(s) 83, 1171
PflMI CCANNNNNTGG 1 cut(s) 487
PfoI TCCNGGA 2 cut(s) 75, 622
PinAI ACCGGT 1 cut(s) 311
PleI GAGTC 4 cut(s) 635, 1459, 2338, 2543
PluTI GGCGCC 1 cut(s) 779
PmaCI CACGTG 1 cut(s) 2318
PmlI CACGTG 1 cut(s) 2318
PpsI GAGTC 4 cut(s) 635, 1459, 2338, 2543
Ppu21I YACGTR 1 cut(s) 2318
PpuMI RGGWCCY 2 cut(s) 1117, 2822
PshBI ATTAAT 1 cut(s) 1227
PsiI TTATAA 1 cut(s) 687
Psp5II RGGWCCY 2 cut(s) 1117, 2822
Psp6I CCWGG 4 cut(s) 75, 480, 2343, 2475
PspCI CACGTG 1 cut(s) 2318
PspEI GGTNACC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 2218
PspGI CCWGG 4 cut(s) 75, 480, 2343, 2475
PspN4I GGNNCC 7 cut(s) 250, 637, 777, 1119, 2342, 2481, 2823
PspPI GGNCC 5 cut(s) 79, 490, 1117, 2340, 2822
PspPPI RGGWCCY 2 cut(s) 1117, 2822
PsrI GAACNNNNNNTAC 2 cut(s) 2151, 2183
PstI CTGCAG 4 cut(s) 1423, 1749, 1798, 2056
PstNI CAGNNNCTG 2 cut(s) 2087, 2495
PsuI RGATCY 5 cut(s) 388, 804, 1741, 2109, 2578
PsyI GACNNNGTC 2 cut(s) 83, 1171
PvuII CAGCTG 3 cut(s) 2051, 2495, 2648
RsaI GTAC 6 cut(s) 206, 915, 1160, 2481, 2499, 2563
RsaNI GTAC 6 cut(s) 205, 914, 1159, 2480, 2498, 2562
SalI GTCGAC 1 cut(s) 1050
SapI GCTCTTC 2 cut(s) 117, 338
SaqAI TTAA 9 cut(s) 303, 1227, 1940, 1982, 2163, 2511, 2627, 2754, 2907
Sau96I GGNCC 5 cut(s) 79, 490, 1117, 2340, 2822
ScaI AGTACT 1 cut(s) 2563
SchI GAGTC 4 cut(s) 636, 1460, 2339, 2543
ScrFI CCNGG 5 cut(s) 77, 482, 624, 2345, 2477
SduI GDGCHC 2 cut(s) 1903, 2300
SfcI CTRYAG 5 cut(s) 1419, 1745, 1794, 2052, 2574
SfoI GGCGCC 1 cut(s) 777
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 4 cut(s) 79, 490, 1117, 2822
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 6 cut(s) 45, 140, 392, 981, 991, 2162
SmoI CTYRAG 6 cut(s) 45, 140, 392, 981, 991, 2162
SpeI ACTAGT 1 cut(s) 2555
SsiI CCGC 9 cut(s) 51, 214, 423, 547, 1277, 1340, 1445, 2113, 2338
SspDI GGCGCC 1 cut(s) 775
SspI AATATT 1 cut(s) 1550
StyD4I CCNGG 5 cut(s) 75, 480, 622, 2343, 2475
StyI CCWWGG 5 cut(s) 66, 111, 1045, 1987, 2841
TaaI ACNGT 4 cut(s) 463, 1013, 2388, 2575
TaiI ACGT 4 cut(s) 166, 228, 1878, 2320
TaqI TCGA 9 cut(s) 106, 141, 147, 228, 672, 1038, 1051, 2228, 2538
TatI WGTACW 5 cut(s) 204, 913, 1158, 2497, 2561
TauI GCSGC 3 cut(s) 54, 425, 2341
TfiI GAWTC 6 cut(s) 887, 1415, 1610, 2144, 2837, 2893
Tru1I TTAA 9 cut(s) 303, 1227, 1940, 1982, 2163, 2511, 2627, 2754, 2907
Tru9I TTAA 9 cut(s) 303, 1227, 1940, 1982, 2163, 2511, 2627, 2754, 2907
TscAI CASTG 5 cut(s) 1573, 2391, 2509, 2578, 2722
TseFI GTSAC 4 cut(s) 1007, 2275, 2546, 2875
TseI GCWGC 8 cut(s) 977, 1747, 1750, 2051, 2489, 2492, 2645, 2654
Tsp45I GTSAC 4 cut(s) 1007, 2275, 2546, 2875
TspDTI ATGAA 9 cut(s) 212, 1334, 1394, 1623, 1658, 1776, 1848, 1989, 2532
TspGWI ACGGA 1 cut(s) 1603
TspRI CASTG 5 cut(s) 1573, 2391, 2509, 2578, 2722
Tth111I GACNNNGTC 2 cut(s) 83, 1171
Van91I CCANNNNNTGG 1 cut(s) 487
Vha464I CTTAAG 1 cut(s) 2162
VpaK11BI GGWCC 4 cut(s) 79, 490, 1117, 2822
VspI ATTAAT 1 cut(s) 1227
XapI RAATTY 6 cut(s) 679, 1662, 2040, 2236, 2305, 2352
XbaI TCTAGA 1 cut(s) 1259
XceI RCATGY 1 cut(s) 1340
XcmI CCANNNNNNNNNTGG 2 cut(s) 73, 1299
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 3 cut(s) 222, 1051, 1465
XmnI GAANNNNTTC 2 cut(s) 1549, 2601
ZraI GACGTC 1 cut(s) 164
ZrmI AGTACT 1 cut(s) 2563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.