Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Reverse (-)

1529350 .. 1537605

Gene Structure 8256 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1013400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2406 bp

ATGGGAGACCTCAAAGCTAACTCCAAGGGGGACCAGAAGGTCGCCAAGCTACCCATCCCTGGCAAGCGAAACATCCTTATCACAAGTGCCTTGCCTTATGTCAATAATGTCCCTCATCTCGGCAATATCATCGGCTGTGTATTGAGTGCCGATGTCTACGCACGCTACTGCCGTCTCCGGGGATACAATACCTTATACATATGCGGAACTGATGAGTACGGCACGGCAACGGAGACCAAAGCTTTGGAAGAGAAGTGTAGCCCCCAAGAAATCTGTGACAAATATCATGCAATTCATAGAGACGTTTATGAGTGGTTTAACATAAGCTTTGATAAATTTGGGCGTACATCGTCACCAGAGCAAACAGAAGTATGCCAAGCTATTTTTAAGAAACTGTTGGAGAATGATTGGCTTACAGAGAACACAATGCAGCAGCTTTACTGTGACACATGCGAACGGTTCTTAGCGGACAGGCTTGTGGAGGGGACATGCCCGACTCAAGGCTGTGGCTATGCTTCTGCACGAGGGGATCAATGTGAGAACTGCGGAAAGCTTTTGAATCCGACAGAACTAAAAGATCCTAAGTGCAAGGTTTGTAAGTCAACTCCACGAATTCGAGATACAAACCACTTGTTTCTGGAACTACCTCTGCTGAAGGATAAGTTGGAACAATATATCAATGAAATGTCAGTGGCTGGGTCATGGAGCCAGAATGCCATTCAAGCGACCAATGCATGGCTCAAAGAGGGGCTTAGGCAGCGGTGTATTACTAGGGATTTAAAGTGGGGTGTTCCGGTTCCACATGATAACTTCAGGGACAAGGTTTTCTACGTGTGGTTTGATGCTCCAATTGGATATGTCTCCATTACTAAATGTTACACTGATGATTGGGAAAAATGGTGGAAGAATCCTGAAAATGTGGAGTTGTATCAGTTTATGGGCAAGGATAATGTGCCATTCCACACAGTGATGTTTCCTTCCACTCTTCTTGGAACTGGTGAAAATTGGACTTTGATGAAGACTATCAGTGTAACCGAGTACTTAAATTATGAATCAGGAAAGTTTTCTAAGAGTAAAGGCATAGGGGTTTTTGGAAACAATGCAAAAGATACAAAAATTCCTGTTGAAGTATGGAGATACTACTTGCTAACTAATAGGCCTGAGGTATCAGACACATTATTTACATGGGCCGACTTGCAAGCAAAACTGAACAGTGAATTGCTGAATAATTTGGGCAACTTCATAAATCGAGTTTTGAGTTTTGTTGCCAAACCTTCAGGTCAAGGATATGGTTCCATTATTCCAGATGCTCCGCAAGCAGAAGCAGATCCTTTAACAGAGAAACTGGCTGAAAAAGTTGGTAAATATGCGGAGCAATATATAGAAGCTATGGAGAAGGTTAAGCTAAAGCAAGGACTCAAAACAGCAATGGCCATTTCTAGTGAAGGGAATGCATATCTGCAAGAAAGTCAGTTCTGGAAGCTGTACAAAGAGGACCAACCTCGTTGCGCTATTGTTATGAGAACTTCAGTCGGACTAGTGCATCTTCTTGCTTGTTTGTTGGAACCCTTTATGCCATCATTTTCTCTCGAAGTATTCAAGCAGCTTAATTTGCCTCCTGAGACACACTTATCACTTTGTGATGATGAGGGAGATATTGATAGGGCAAGACGGCCTTGGGAGATTGTACCTATTGGTCACAAGATTGGGAAACCAGAGCCATTGTTCAAGGAATTGAAAGATGAAGAAGTAGAATTCTGGAGGCAGAAGTTTGCTGGAAGTCAAGCTGACAGAAAAGAGAGGGAAGAAGCTGAAGCAATAAAGGTGGCTGCACAACTCAAAAAAACGAAAGTTTCAGACAGTACTGGAAAGAAAAAGCAACAGCCCACAAAATCTGCAGCTGAAGCAGAAATTTCTATTAGTAGGCTTGATATCCGCGTTGGCCTCATTACTAAAGCTCAGAAGCATCCTGATGCTGATTCACTGTACGTAGAGGATATTGATGTGGGTGAAGGACAAACCAGGACAGTGGTCAGTGGGCTTGTCAAATATATACCCCTTGAAAAAATGCAGAACCGGAAAGTATGTGTTCTTTGCAATCTGAAGCCAGCAAACATGCGGGGTATTAAGTCAGAAGCAATGGTTCTTGCTGCTTCAAACAGTGACCACACCACAGTTGAACTGGTTGAACCACCAGAAGGTGCACAAGTTGGCGAGAGAGTTACATTTCCCGGGTTTGTAGGTGAGCCTGATGAGGTTTTGAACCCGAAGAAGAAGGTGTGGGAGACCTTGCAGGTGGATCTGCACACGAACACTGAACTAGTGGCGTGCTACAAAGATGTTCCGCTCACAACATCAGCTGGTGTTTGCACCGTGTCTTCTATTGCCGGTGGGTCAATTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g tRNA_bind

Protein Analysis

802

Amino Acids

90.17

Weight (kDa)

6.18

Isoelectric Point (pI)

26.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	25 - 419	7.8e-157	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	26 - 275	9.3e-10	tRNA synthetases class I (I, L, M and V)
Anticodon_3	PF19303	443 - 595	1.2e-21	Anticodon binding domain of methionyl tRNA ligase
tRNA_bind	PF01588	645 - 740	9.2e-33	Putative tRNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2284
AasI	GACNNNNNNGTC	1 cut(s)	38
Acc36I	ACCTGC	1 cut(s)	2284
AccB7I	CCANNNNNTGG	1 cut(s)	735
AccBSI	CCGCTC	1 cut(s)	2347
AccI	GTMKAC	1 cut(s)	156
AccII	CGCG	1 cut(s)	1938
AciI	CCGC	9 cut(s)	204, 467, 546, 760, 1313, 1370, 1936, 2119, 2345
AclWI	GGATC	4 cut(s)	537, 572, 1322, 2307
AcoI	YGGCCR	1 cut(s)	1431
AcsI	RAATTY	5 cut(s)	335, 612, 1116, 1754, 1911
AcuI	CTGAAG	7 cut(s)	674, 796, 1260, 1512, 1833, 1923, 2123
AdeI	CACNNNGTG	2 cut(s)	967, 1640
AfaI	GTAC	7 cut(s)	218, 346, 1040, 1487, 1689, 1864, 1988
AfiI	CCNNNNNNNGG	6 cut(s)	59, 119, 178, 500, 735, 2198
AflIII	ACRYGT	1 cut(s)	831
AhlI	ACTAGT	2 cut(s)	1537, 2320
AjnI	CCWGG	2 cut(s)	58, 2021
AjuI	GAANNNNNNNTTGG	2 cut(s)	647, 679
Alw21I	GWGCWC	1 cut(s)	2206
Alw26I	GTCTC	6 cut(s)	179, 227, 294, 865, 1616, 2279
Alw44I	GTGCAC	1 cut(s)	2202
AlwI	GGATC	4 cut(s)	537, 572, 1322, 2307
AlwNI	CAGNNNCTG	1 cut(s)	695
Ama87I	CYCGRG	1 cut(s)	2231
AoxI	GGCC	5 cut(s)	1157, 1188, 1431, 1673, 1942
ApaLI	GTGCAC	1 cut(s)	2202
ApeKI	GCWGC	7 cut(s)	430, 433, 757, 1603, 1829, 1898, 2150
ApoI	RAATTY	5 cut(s)	335, 612, 1116, 1754, 1911
ArsI	GACNNNNNNTTYG	2 cut(s)	226, 258
Asp700I	GAANNNNTTC	1 cut(s)	1063
AspLEI	GCGC	1 cut(s)	1511
AspS9I	GGNCC	3 cut(s)	31, 1188, 1495
AsuC2I	CCSGG	3 cut(s)	179, 2232, 2233
AsuHPI	GGTGA	4 cut(s)	345, 1010, 2021, 2255
AvaI	CYCGRG	1 cut(s)	2231
AvaII	GGWCC	2 cut(s)	31, 1495
AxyI	CCTNAGG	1 cut(s)	1161
BaeGI	GKGCMC	1 cut(s)	2206
BalI	TGGCCA	1 cut(s)	1433
BauI	CACGAG	1 cut(s)	522
BbsI	GAAGAC	2 cut(s)	1025, 2370
Bbv12I	GWGCWC	1 cut(s)	2206
BbvI	GCAGC	7 cut(s)	442, 445, 769, 1615, 1816, 1910, 2137
BccI	CCATC	2 cut(s)	62, 1585
BceAI	ACGGC	4 cut(s)	156, 235, 240, 1688
BcgI	CGANNNNNNTGC	2 cut(s)	112, 146
BciT130I	CCWGG	2 cut(s)	60, 2023
BciVI	GTATCC	1 cut(s)	176
BcnI	CCSGG	3 cut(s)	179, 2232, 2233
BcoDI	GTCTC	6 cut(s)	179, 227, 294, 865, 1616, 2279
BcuI	ACTAGT	2 cut(s)	1537, 2320
BfaI	CTAG	4 cut(s)	771, 1440, 1538, 2321
BfmI	CTRYAG	1 cut(s)	1896
BfuAI	ACCTGC	1 cut(s)	2284
BfuI	GTATCC	1 cut(s)	176
BisI	GCNGC	7 cut(s)	431, 434, 758, 1604, 1830, 1899, 2151
BlsI	GCNGC	7 cut(s)	432, 435, 759, 1605, 1831, 1900, 2152
BmcAI	AGTACT	2 cut(s)	1040, 1864
Bme1390I	CCNGG	5 cut(s)	60, 179, 2023, 2232, 2233
Bme18I	GGWCC	2 cut(s)	31, 1495
BmeT110I	CYCGRG	1 cut(s)	2231
BmgT120I	GGNCC	3 cut(s)	31, 1188, 1495
BmiI	GGNNCC	5 cut(s)	32, 707, 798, 1294, 1566
BmrFI	CCNGG	5 cut(s)	60, 179, 2023, 2232, 2233
BmsI	GCATC	5 cut(s)	832, 1297, 1552, 1963, 1975
BoxI	GACNNNNGTC	1 cut(s)	2030
BpiI	GAAGAC	2 cut(s)	1025, 2370
BpmI	CTGGAG	1 cut(s)	1780
Bpu10I	CCTNAGC	1 cut(s)	752
BpuEI	CTTGAG	1 cut(s)	483
BpuMI	CCSGG	3 cut(s)	179, 2232, 2233
BsaAI	YACGTR	2 cut(s)	832, 1990
BsaI	GGTCTC	2 cut(s)	227, 2279
BsaJI	CCNNGG	5 cut(s)	24, 58, 178, 1676, 2231
BsaWI	WCCGGW	2 cut(s)	793, 2076
Bsc4I	CCNNNNNNNGG	6 cut(s)	59, 119, 178, 500, 735, 2198
Bse118I	RCCGGY	1 cut(s)	2387
Bse1I	ACTGG	4 cut(s)	1000, 1350, 1870, 2187
Bse21I	CCTNAGG	1 cut(s)	1161
Bse3DI	GCAATG	2 cut(s)	1434, 2145
BseBI	CCWGG	2 cut(s)	60, 2023
BseDI	CCNNGG	5 cut(s)	24, 58, 178, 1676, 2231
BseGI	GGATG	3 cut(s)	54, 72, 1966
BseLI	CCNNNNNNNGG	6 cut(s)	59, 119, 178, 500, 735, 2198
BseMI	GCAATG	2 cut(s)	1434, 2145
BseMII	CTCAG	3 cut(s)	1152, 1611, 1973
BseNI	ACTGG	4 cut(s)	1000, 1350, 1870, 2187
BseSI	GKGCMC	1 cut(s)	2206
BseXI	GCAGC	7 cut(s)	442, 445, 769, 1615, 1816, 1910, 2137
BseYI	CCCAGC	1 cut(s)	695
BsgI	GTGCAG	3 cut(s)	504, 1815, 2288
Bsh1236I	CGCG	1 cut(s)	1938
BshFI	GGCC	5 cut(s)	1159, 1190, 1433, 1675, 1944
BsiHKAI	GWGCWC	1 cut(s)	2206
BsiHKCI	CYCGRG	1 cut(s)	2231
BsiSI	CCGG	5 cut(s)	178, 794, 2077, 2232, 2388
BslFI	GGGAC	4 cut(s)	44, 95, 499, 830
BslI	CCNNNNNNNGG	6 cut(s)	59, 119, 178, 500, 735, 2198
BsmAI	GTCTC	6 cut(s)	179, 227, 294, 865, 1616, 2279
BsmBI	CGTCTC	2 cut(s)	179, 294
BsmFI	GGGAC	4 cut(s)	44, 95, 499, 830
BsmI	GAATGC	2 cut(s)	718, 1456
BsnI	GGCC	5 cut(s)	1159, 1190, 1433, 1675, 1944
Bso31I	GGTCTC	2 cut(s)	227, 2279
BsoBI	CYCGRG	1 cut(s)	2231
Bsp1286I	GDGCHC	1 cut(s)	2206
Bsp1407I	TGTACA	1 cut(s)	1485
Bsp143I	GATC	4 cut(s)	529, 577, 1327, 2299
BspACI	CCGC	9 cut(s)	204, 467, 546, 760, 1313, 1370, 1936, 2119, 2345
BspANI	GGCC	5 cut(s)	1159, 1190, 1433, 1675, 1944
BspCNI	CTCAG	3 cut(s)	1153, 1612, 1972
BspFNI	CGCG	1 cut(s)	1938
BspLI	GGNNCC	5 cut(s)	32, 707, 798, 1294, 1566
BspMAI	CTGCAG	1 cut(s)	1900
BspMI	ACCTGC	1 cut(s)	2284
BspPI	GGATC	4 cut(s)	537, 572, 1322, 2307
BspTNI	GGTCTC	2 cut(s)	227, 2279
BsrBI	CCGCTC	1 cut(s)	2347
BsrDI	GCAATG	2 cut(s)	1434, 2145
BsrFI	RCCGGY	1 cut(s)	2387
BsrGI	TGTACA	1 cut(s)	1485
BsrI	ACTGG	4 cut(s)	1000, 1350, 1870, 2187
BssAI	RCCGGY	1 cut(s)	2387
BssECI	CCNNGG	5 cut(s)	24, 58, 178, 1676, 2231
BssMI	GATC	4 cut(s)	529, 577, 1327, 2299
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	2 cut(s)	24, 1676
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	2 cut(s)	60, 2023
Bst6I	CTCTTC	2 cut(s)	243, 990
BstAUI	TGTACA	1 cut(s)	1485
BstBAI	YACGTR	2 cut(s)	832, 1990
BstC8I	GCNNGC	6 cut(s)	65, 163, 1200, 1317, 2109, 2329
BstDEI	CTNAG	7 cut(s)	463, 582, 752, 1068, 1161, 1620, 1959
BstF5I	GGATG	3 cut(s)	54, 72, 1966
BstFNI	CGCG	1 cut(s)	1938
BstHHI	GCGC	1 cut(s)	1511
BstKTI	GATC	4 cut(s)	532, 580, 1330, 2302
BstMAI	GTCTC	6 cut(s)	179, 227, 294, 865, 1616, 2279
BstMBI	GATC	4 cut(s)	529, 577, 1327, 2299
BstMWI	GCNNNNNNNGC	6 cut(s)	722, 731, 757, 1316, 1612, 1904
BstNI	CCWGG	2 cut(s)	60, 2023
BstNSI	RCATGY	3 cut(s)	453, 492, 2119
BstPAI	GACNNNNGTC	1 cut(s)	2030
BstSCI	CCNGG	5 cut(s)	58, 177, 2021, 2230, 2231
BstSFI	CTRYAG	1 cut(s)	1896
BstSLI	GKGCMC	1 cut(s)	2206
BstSNI	TACGTA	1 cut(s)	1990
BstUI	CGCG	1 cut(s)	1938
BstV1I	GCAGC	7 cut(s)	442, 445, 769, 1615, 1816, 1910, 2137
BstV2I	GAAGAC	2 cut(s)	1025, 2370
BstX2I	RGATCY	3 cut(s)	577, 1327, 2299
BstXI	CCANNNNNNTGG	2 cut(s)	244, 2029
BstYI	RGATCY	3 cut(s)	577, 1327, 2299
Bsu36I	CCTNAGG	1 cut(s)	1161
BsuI	GTATCC	1 cut(s)	176
BsuRI	GGCC	5 cut(s)	1159, 1190, 1433, 1675, 1944
BtsCI	GGATG	3 cut(s)	54, 72, 1966
BveI	ACCTGC	1 cut(s)	2284
Cac8I	GCNNGC	6 cut(s)	65, 163, 1200, 1317, 2109, 2329
CaiI	CAGNNNCTG	1 cut(s)	695
CfoI	GCGC	1 cut(s)	1511
Cfr10I	RCCGGY	1 cut(s)	2387
Cfr13I	GGNCC	3 cut(s)	31, 1188, 1495
Cfr9I	CCCGGG	1 cut(s)	2231
Csp6I	GTAC	7 cut(s)	217, 345, 1039, 1486, 1688, 1863, 1987
CspCI	CAANNNNNGTGG	4 cut(s)	970, 1005, 1806, 1841
CviAII	CATG	8 cut(s)	287, 450, 489, 702, 735, 803, 1185, 2116
CviQI	GTAC	7 cut(s)	217, 345, 1039, 1486, 1688, 1863, 1987
DdeI	CTNAG	7 cut(s)	463, 582, 752, 1068, 1161, 1620, 1959
DpnI	GATC	4 cut(s)	531, 579, 1329, 2301
DpnII	GATC	4 cut(s)	529, 577, 1327, 2299
DraI	TTTAAA	1 cut(s)	780
DraIII	CACNNNGTG	2 cut(s)	967, 1640
DrdI	GACNNNNNNGTC	1 cut(s)	38
DseDI	GACNNNNNNGTC	1 cut(s)	38
EaeI	YGGCCR	1 cut(s)	1431
Eam1104I	CTCTTC	2 cut(s)	243, 990
EarI	CTCTTC	2 cut(s)	243, 990
Eco105I	TACGTA	1 cut(s)	1990
Eco130I	CCWWGG	2 cut(s)	24, 1676
Eco147I	AGGCCT	1 cut(s)	1159
Eco31I	GGTCTC	2 cut(s)	227, 2279
Eco32I	GATATC	1 cut(s)	1933
Eco47I	GGWCC	2 cut(s)	31, 1495
Eco57I	CTGAAG	7 cut(s)	674, 796, 1260, 1512, 1833, 1923, 2123
Eco81I	CCTNAGG	1 cut(s)	1161
Eco88I	CYCGRG	1 cut(s)	2231
EcoRI	GAATTC	2 cut(s)	612, 1754
EcoRII	CCWGG	2 cut(s)	58, 2021
EcoRV	GATATC	1 cut(s)	1933
EcoT14I	CCWWGG	2 cut(s)	24, 1676
EcoT22I	ATGCAT	2 cut(s)	736, 1456
ErhI	CCWWGG	2 cut(s)	24, 1676
Esp3I	CGTCTC	2 cut(s)	179, 294
FaeI	CATG	8 cut(s)	290, 453, 492, 705, 738, 806, 1188, 2119
FalI	AAGNNNNNCTT	4 cut(s)	735, 767, 1660, 1692
FaqI	GGGAC	4 cut(s)	44, 95, 499, 830
FatI	CATG	8 cut(s)	286, 449, 488, 701, 734, 802, 1184, 2115
FauI	CCCGC	1 cut(s)	2112
FauNDI	CATATG	1 cut(s)	200
FblI	GTMKAC	1 cut(s)	156
Fnu4HI	GCNGC	7 cut(s)	431, 434, 758, 1604, 1830, 1899, 2151
FokI	GGATG	3 cut(s)	41, 59, 1953
Fsp4HI	GCNGC	7 cut(s)	431, 434, 758, 1604, 1830, 1899, 2151
FspBI	CTAG	4 cut(s)	771, 1440, 1538, 2321
GlaI	GCGC	1 cut(s)	1510
GluI	GCNGC	7 cut(s)	431, 434, 758, 1604, 1830, 1899, 2151
GsaI	CCCAGC	1 cut(s)	699
GsuI	CTGGAG	1 cut(s)	1780
HaeIII	GGCC	5 cut(s)	1159, 1190, 1433, 1675, 1944
HapII	CCGG	5 cut(s)	178, 794, 2077, 2232, 2388
HhaI	GCGC	1 cut(s)	1511
Hin1II	CATG	8 cut(s)	290, 453, 492, 705, 738, 806, 1188, 2119
Hin6I	GCGC	1 cut(s)	1509
HinP1I	GCGC	1 cut(s)	1509
HincII	GTYRAC	1 cut(s)	603
HindII	GTYRAC	1 cut(s)	603
HindIII	AAGCTT	3 cut(s)	240, 325, 551
HinfI	GANTC	6 cut(s)	496, 559, 907, 1052, 1416, 1979
HpaII	CCGG	5 cut(s)	178, 794, 2077, 2232, 2388
HphI	GGTGA	4 cut(s)	345, 1010, 2021, 2255
Hpy166II	GTNNAC	3 cut(s)	157, 603, 2204
Hpy188I	TCNGA	7 cut(s)	564, 1171, 1535, 1858, 1962, 2103, 2134
Hpy8I	GTNNAC	3 cut(s)	157, 603, 2204
HpyAV	CCTTC	9 cut(s)	31, 649, 987, 1284, 1390, 1439, 2006, 2192, 2269
HpyCH4IV	ACGT	3 cut(s)	303, 831, 1989
HpyF10VI	GCNNNNNNNGC	6 cut(s)	722, 731, 757, 1316, 1612, 1904
HpyF3I	CTNAG	7 cut(s)	463, 582, 752, 1068, 1161, 1620, 1959
HpySE526I	ACGT	3 cut(s)	303, 831, 1989
Hsp92II	CATG	8 cut(s)	290, 453, 492, 705, 738, 806, 1188, 2119
HspAI	GCGC	1 cut(s)	1509
Kzo9I	GATC	4 cut(s)	529, 577, 1327, 2299
LmnI	GCTCC	4 cut(s)	705, 850, 1315, 1372
Lsp1109I	GCAGC	7 cut(s)	442, 445, 769, 1615, 1816, 1910, 2137
LweI	GCATC	5 cut(s)	832, 1297, 1552, 1963, 1975
MaeI	CTAG	4 cut(s)	771, 1440, 1538, 2321
MaeII	ACGT	3 cut(s)	303, 831, 1989
MaeIII	GTNAC	8 cut(s)	275, 351, 443, 875, 1030, 1697, 2162, 2221
MalI	GATC	4 cut(s)	531, 579, 1329, 2301
MbiI	CCGCTC	1 cut(s)	2347
MboI	GATC	4 cut(s)	529, 577, 1327, 2299
MfeI	CAATTG	1 cut(s)	849
MflI	RGATCY	3 cut(s)	577, 1327, 2299
MhlI	GDGCHC	1 cut(s)	2206
MlsI	TGGCCA	1 cut(s)	1433
MluNI	TGGCCA	1 cut(s)	1433
MlyI	GAGTC	2 cut(s)	490, 1410
MmeI	TCCRAC	5 cut(s)	378, 587, 645, 1513, 1542
Mox20I	TGGCCA	1 cut(s)	1433
Mph1103I	ATGCAT	2 cut(s)	736, 1456
MroXI	GAANNNNTTC	1 cut(s)	1063
MscI	TGGCCA	1 cut(s)	1433
MseI	TTAA	9 cut(s)	318, 387, 779, 1043, 1334, 1401, 1608, 2127, 2400
MslI	CAYNNNNRTG	2 cut(s)	968, 1971
Msp20I	TGGCCA	1 cut(s)	1433
MspA1I	CMGCKG	3 cut(s)	760, 1901, 2360
MspI	CCGG	5 cut(s)	178, 794, 2077, 2232, 2388
MspR9I	CCNGG	5 cut(s)	60, 179, 2023, 2232, 2233
MunI	CAATTG	1 cut(s)	849
Mva1269I	GAATGC	2 cut(s)	718, 1456
MvaI	CCWGG	2 cut(s)	60, 2023
MvnI	CGCG	1 cut(s)	1938
MwoI	GCNNNNNNNGC	6 cut(s)	722, 731, 757, 1316, 1612, 1904
NciI	CCSGG	3 cut(s)	179, 2232, 2233
NdeI	CATATG	1 cut(s)	200
NdeII	GATC	4 cut(s)	529, 577, 1327, 2299
NlaIII	CATG	8 cut(s)	290, 453, 492, 705, 738, 806, 1188, 2119
NlaIV	GGNNCC	5 cut(s)	32, 707, 798, 1294, 1566
NmeAIII	GCCGAG	1 cut(s)	99
NmuCI	GTSAC	5 cut(s)	275, 351, 443, 1697, 2162
NsiI	ATGCAT	2 cut(s)	736, 1456
NspI	RCATGY	3 cut(s)	453, 492, 2119
PaqCI	CACCTGC	1 cut(s)	2284
PceI	AGGCCT	1 cut(s)	1159
PcsI	WCGNNNNNNNCGW	1 cut(s)	169
PctI	GAATGC	2 cut(s)	718, 1456
PdmI	GAANNNNTTC	1 cut(s)	1063
PfeI	GAWTC	4 cut(s)	559, 907, 1052, 1979
PflMI	CCANNNNNTGG	1 cut(s)	735
PkrI	GCNGC	7 cut(s)	432, 435, 759, 1605, 1831, 1900, 2152
PleI	GAGTC	2 cut(s)	490, 1410
PpsI	GAGTC	2 cut(s)	490, 1410
Ppu21I	YACGTR	2 cut(s)	832, 1990
PshAI	GACNNNNGTC	1 cut(s)	2030
Psp6I	CCWGG	2 cut(s)	58, 2021
PspFI	CCCAGC	1 cut(s)	695
PspGI	CCWGG	2 cut(s)	58, 2021
PspN4I	GGNNCC	5 cut(s)	32, 707, 798, 1294, 1566
PspPI	GGNCC	3 cut(s)	31, 1188, 1495
PstI	CTGCAG	1 cut(s)	1900
PstNI	CAGNNNCTG	1 cut(s)	695
PsuI	RGATCY	3 cut(s)	577, 1327, 2299
PvuII	CAGCTG	2 cut(s)	1901, 2360
RsaI	GTAC	7 cut(s)	218, 346, 1040, 1487, 1689, 1864, 1988
RsaNI	GTAC	7 cut(s)	217, 345, 1039, 1486, 1688, 1863, 1987
RseI	CAYNNNNRTG	2 cut(s)	968, 1971
SaqAI	TTAA	9 cut(s)	318, 387, 779, 1043, 1334, 1401, 1608, 2127, 2400
SatI	GCNGC	7 cut(s)	431, 434, 758, 1604, 1830, 1899, 2151
Sau3AI	GATC	4 cut(s)	529, 577, 1327, 2299
Sau96I	GGNCC	3 cut(s)	31, 1188, 1495
ScaI	AGTACT	2 cut(s)	1040, 1864
SchI	GAGTC	2 cut(s)	490, 1410
ScrFI	CCNGG	5 cut(s)	60, 179, 2023, 2232, 2233
SduI	GDGCHC	1 cut(s)	2206
SfaNI	GCATC	5 cut(s)	832, 1297, 1552, 1963, 1975
SfcI	CTRYAG	1 cut(s)	1896
SinI	GGWCC	2 cut(s)	31, 1495
SmaI	CCCGGG	1 cut(s)	2233
SmiMI	CAYNNNNRTG	2 cut(s)	968, 1971
SmlI	CTYRAG	1 cut(s)	498
SmoI	CTYRAG	1 cut(s)	498
SnaBI	TACGTA	1 cut(s)	1990
SpeI	ACTAGT	2 cut(s)	1537, 2320
SseBI	AGGCCT	1 cut(s)	1159
SsiI	CCGC	9 cut(s)	204, 467, 546, 760, 1313, 1370, 1936, 2119, 2345
SspMI	CTAG	4 cut(s)	771, 1440, 1538, 2321
StuI	AGGCCT	1 cut(s)	1159
StyD4I	CCNGG	5 cut(s)	58, 177, 2021, 2230, 2231
StyI	CCWWGG	2 cut(s)	24, 1676
TaiI	ACGT	3 cut(s)	306, 834, 1992
TaqI	TCGA	3 cut(s)	616, 1249, 1590
TatI	WGTACW	3 cut(s)	1038, 1485, 1862
TfiI	GAWTC	4 cut(s)	559, 907, 1052, 1979
Tru1I	TTAA	9 cut(s)	318, 387, 779, 1043, 1334, 1401, 1608, 2127, 2400
Tru9I	TTAA	9 cut(s)	318, 387, 779, 1043, 1334, 1401, 1608, 2127, 2400
TseFI	GTSAC	5 cut(s)	275, 351, 443, 1697, 2162
TseI	GCWGC	7 cut(s)	430, 433, 757, 1603, 1829, 1898, 2150
Tsp45I	GTSAC	5 cut(s)	275, 351, 443, 1697, 2162
TspDTI	ATGAA	6 cut(s)	284, 696, 1031, 1065, 1231, 1758
TspGWI	ACGGA	1 cut(s)	245
TspMI	CCCGGG	1 cut(s)	2231
Van91I	CCANNNNNTGG	1 cut(s)	735
VneI	GTGCAC	1 cut(s)	2202
VpaK11BI	GGWCC	2 cut(s)	31, 1495
XapI	RAATTY	5 cut(s)	335, 612, 1116, 1754, 1911
XceI	RCATGY	3 cut(s)	453, 492, 2119
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2179
XmaI	CCCGGG	1 cut(s)	2231
XmiI	GTMKAC	1 cut(s)	156
XmnI	GAANNNNTTC	1 cut(s)	1063
XspI	CTAG	4 cut(s)	771, 1440, 1538, 2321
ZrmI	AGTACT	2 cut(s)	1040, 1864
Zsp2I	ATGCAT	2 cut(s)	736, 1456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.