Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Forward (+)

32291812 .. 32298565

Gene Structure 6754 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG184300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGGGAGACCTCGCCGTTACTTCCAAGGAGGGCCCTAAGCTCCCCATCCCCGGCCAACGCAACGTCCTCATCACCGGCGCGCTGCCCTACGTCAACAACGTCCCTCATCTCGGCAACATCATCGGCTGCGTTCTCAGCGCCGACGTCTTCGCTCGCTACTGCCGTCTCCGGGGATACAACACCGTCTATATCTGCGGCACCGATGAGTACGGCACCGCCACGGAGACCAAAGCCATGGAGGAGAAGTGTAGCCCCAAGGAGATCTGCGATAAGTACTATGCGATTCATCGGCAGGTGTATGAGTGGTTCAATATTAGTTTTGACAAATTCGGGCGAACTTCGACGCCGGAGCAGACGGAGGTGTGCCAAGCGATTTTCAATAGGCTGCTGGAGAATAATTGGCTTTCTGAGAACACAATGCAGCAGCTATATTGTGATACTTGCACACGGTTTTTGGCCGACAGGCTCGTGGAGGGAACGTGCCCAACGCAAGGTTGTGATTATACTTCTGCAAGAGGAGATCAGTGTGAGAACTGTGGGAAGCTTTTAAACCCCACAGAATTAAAGGATCCAAAGTGCAAGGTCTGTAAGTCGACTCCACGGATTCGTGATACAAACCATTTGTTTCTGGAACTGCCTTTGCTGAAGGATAGGTTGGAAGAGTATATTAATAAAATGTCAGTGGAGGGGTCTTGGAGCCAGAATGCCATTCATTCAACAAATGCGTGGCTTAGAGAGGGGCTTAAACAACGGTGTATCACCAGGGATCTGAAGTGGGGGGTTCCAGTTCCACATGATAACTTCAGGGACAAGGTTTTCTATGTGTGGTTTGATGCGCCTATTGGATATGTTTCAATTACTAAATGCTACACTCCAGATTGGGAGAAATGGTGGAAGAATCCAGAGGATGTGGAGTTGTATCAGTTTATGGGCAAAGATAATGTGCCATTCCACACGGTGATGTTTCCTTCCACACTTCTTGGAACTGGTGAAAATTGGACCTTAATGAAGACAATTAGTGTTACTGAATACTTGAACTATGAAGCAGGAAAGTTTTCCAAGAGTAAAGGGGTTGGAGTATTTGGAAACGATGCCAAAGATACAAACATTCCTGTTGAAGTATGGCGATATTACTTGCTAACCAATAGGCCCGAGGTGTCGGATACATTATTTACATGGGCGGATTTGCAAGCGAAGCTCAACAGTGAATTGCTGAATAATTTGGGCAACTTCATTAACCGAGTTTTGAGTTTTGTTGCCAAACCTTTAGGTCAAGGATATGACTCCATTATTCCAGATGCTCAAGATGCCATCTCTGATCCTTTGACAGAGAAAATAGCTGAAAAAGTGGGTAAATGTGTGGAGCAATATGTAGAGGCCATGGAGAAGGTAAAACTAAAGCAAGGACTCAAACTAGCAATGAGCATTTCAAGTGAAGGGAATGCATATCTGCAAGAAAGTCAGTTCTGGAAGCTTTACAAGGAGGATCAAACTCGCTGCTCCATTGTTATGAGAACTGCAGTGGGGCTAGTGCATCTTCTTGCTTGCTTATTAGAACCCTTCATGCCATCTTTTTCTGTTGAGGTATTTAAGCAGCTTAATTTGTCTCCTGAGACGCACATATCACTTTGTGATGATAAAGGTGATATTGATAGGGCAAGACGGCCATGGGAGATTGTACCTAGAGGTCACAAGATTGGGAAACCAGAGCCGTTGTTCAAGGAATTGGTATTGTCTCTGTTGTGCTCTGTCTTTTACTTATTGATTGGGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g tRNA_bind

Protein Analysis

591

Amino Acids

67.44

Weight (kDa)

5.88

Isoelectric Point (pI)

28.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	22 - 416	1.7e-154	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	27 - 265	8.4e-09	tRNA synthetases class I (I, L, M and V)
Anticodon_3	PF19303	443 - 575	4.7e-15	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	283
AatII	GACGTC	1 cut(s)	147
Acc36I	ACCTGC	1 cut(s)	283
AccB1I	GGYRCC	2 cut(s)	197, 212
AccI	GTMKAC	1 cut(s)	593
AccII	CGCG	1 cut(s)	80
AciI	CCGC	3 cut(s)	195, 216, 1181
AclWI	GGATC	5 cut(s)	563, 576, 774, 1313, 1494
AcoI	YGGCCR	3 cut(s)	52, 456, 1664
AcsI	RAATTY	1 cut(s)	326
AcuI	CTGAAG	3 cut(s)	665, 787, 791
AcyI	GRCGYC	2 cut(s)	144, 344
AdeI	CACNNNGTG	2 cut(s)	958, 1631
AfaI	GTAC	3 cut(s)	209, 275, 1680
AfiI	CCNNNNNNNGG	4 cut(s)	50, 110, 169, 491
AgsI	TTSAA	8 cut(s)	310, 379, 717, 855, 1036, 1118, 1431, 1720
AjnI	CCWGG	1 cut(s)	761
AjuI	GAANNNNNNNTTGG	2 cut(s)	638, 670
AluBI	AGCT	7 cut(s)	40, 427, 544, 1198, 1340, 1474, 1597
AluI	AGCT	7 cut(s)	40, 427, 544, 1198, 1340, 1474, 1597
Alw21I	GWGCWC	1 cut(s)	1748
Alw26I	GTCTC	5 cut(s)	170, 218, 1607, 1611, 1740
AlwI	GGATC	5 cut(s)	563, 576, 774, 1313, 1494
Ama87I	CYCGRG	1 cut(s)	1151
AoxI	GGCC	6 cut(s)	31, 52, 456, 1148, 1377, 1664
ApaI	GGGCCC	1 cut(s)	35
ApeKI	GCWGC	7 cut(s)	82, 126, 385, 421, 424, 1497, 1594
ApoI	RAATTY	1 cut(s)	326
AseI	ATTAAT	1 cut(s)	669
Asp700I	GAANNNNTTC	1 cut(s)	1054
AspLEI	GCGC	4 cut(s)	80, 82, 140, 838
AspS9I	GGNCC	4 cut(s)	31, 32, 999, 1149
AsuC2I	CCSGG	2 cut(s)	51, 170
AsuHPI	GGTGA	5 cut(s)	64, 751, 970, 1001, 1655
AvaI	CYCGRG	1 cut(s)	1151
AvaII	GGWCC	1 cut(s)	999
BaeGI	GKGCMC	2 cut(s)	35, 485
BamHI	GGATCC	1 cut(s)	568
BanI	GGYRCC	2 cut(s)	197, 212
BanII	GRGCYC	1 cut(s)	35
BauI	CACGAG	1 cut(s)	467
BbsI	GAAGAC	2 cut(s)	139, 1016
Bbv12I	GWGCWC	1 cut(s)	1748
BbvI	GCAGC	7 cut(s)	69, 113, 372, 433, 436, 1484, 1606
BccI	CCATC	3 cut(s)	53, 1319, 1576
BceAI	ACGGC	4 cut(s)	147, 226, 1679, 1696
BcgI	CGANNNNNNTGC	2 cut(s)	103, 137
BciT130I	CCWGG	1 cut(s)	763
BciVI	GTATCC	2 cut(s)	167, 1156
BcnI	CCSGG	2 cut(s)	51, 170
BcoDI	GTCTC	5 cut(s)	170, 218, 1607, 1611, 1740
BfaI	CTAG	3 cut(s)	1415, 1529, 1683
BfmI	CTRYAG	1 cut(s)	1518
BfoI	RGCGCY	1 cut(s)	141
BfuAI	ACCTGC	1 cut(s)	283
BfuI	GTATCC	2 cut(s)	167, 1156
BglII	AGATCT	1 cut(s)	261
BisI	GCNGC	8 cut(s)	83, 127, 196, 386, 422, 425, 1498, 1595
BlsI	GCNGC	8 cut(s)	84, 128, 197, 387, 423, 426, 1499, 1596
BmcAI	AGTACT	1 cut(s)	275
Bme1390I	CCNGG	3 cut(s)	51, 170, 763
Bme18I	GGWCC	1 cut(s)	999
BmeT110I	CYCGRG	1 cut(s)	1151
BmgT120I	GGNCC	4 cut(s)	31, 32, 999, 1149
BmiI	GGNNCC	6 cut(s)	33, 199, 214, 570, 698, 783
BmrFI	CCNGG	3 cut(s)	51, 170, 763
BmsI	GCATC	5 cut(s)	823, 1081, 1288, 1297, 1543
BpiI	GAAGAC	2 cut(s)	139, 1016
BpmI	CTGGAG	2 cut(s)	410, 858
Bpu10I	CCTNAGC	1 cut(s)	36
BpuEI	CTTGAG	1 cut(s)	1287
BpuMI	CCSGG	2 cut(s)	51, 170
BsaHI	GRCGYC	2 cut(s)	144, 344
BsaI	GGTCTC	1 cut(s)	218
BsaXI	ACNNNNNCTCC	6 cut(s)	230, 260, 464, 494, 875, 905
Bsc4I	CCNNNNNNNGG	4 cut(s)	50, 110, 169, 491
Bse118I	RCCGGY	1 cut(s)	74
Bse1I	ACTGG	2 cut(s)	785, 991
Bse3DI	GCAATG	1 cut(s)	1425
BseBI	CCWGG	1 cut(s)	763
BseGI	GGATG	2 cut(s)	45, 913
BseLI	CCNNNNNNNGG	4 cut(s)	50, 110, 169, 491
BseMI	GCAATG	1 cut(s)	1425
BseMII	CTCAG	3 cut(s)	148, 399, 1602
BseNI	ACTGG	2 cut(s)	785, 991
BsePI	GCGCGC	1 cut(s)	78
BseRI	GAGGAG	2 cut(s)	254, 531
BseSI	GKGCMC	2 cut(s)	35, 485
BseXI	GCAGC	7 cut(s)	69, 113, 372, 433, 436, 1484, 1606
Bsh1236I	CGCG	1 cut(s)	80
BshFI	GGCC	6 cut(s)	33, 54, 458, 1150, 1379, 1666
BshNI	GGYRCC	2 cut(s)	197, 212
BsiHKAI	GWGCWC	1 cut(s)	1748
BsiHKCI	CYCGRG	1 cut(s)	1151
BsiSI	CCGG	4 cut(s)	51, 75, 169, 347
BslFI	GGGAC	2 cut(s)	86, 821
BslI	CCNNNNNNNGG	4 cut(s)	50, 110, 169, 491
BsmAI	GTCTC	5 cut(s)	170, 218, 1607, 1611, 1740
BsmBI	CGTCTC	2 cut(s)	170, 1607
BsmFI	GGGAC	2 cut(s)	86, 821
BsmI	GAATGC	2 cut(s)	709, 1447
BsnI	GGCC	6 cut(s)	33, 54, 458, 1150, 1379, 1666
Bso31I	GGTCTC	1 cut(s)	218
BsoBI	CYCGRG	1 cut(s)	1151
Bsp120I	GGGCCC	1 cut(s)	31
Bsp1286I	GDGCHC	3 cut(s)	35, 485, 1748
Bsp143I	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
Bsp19I	CCATGG	3 cut(s)	234, 1380, 1667
BspACI	CCGC	3 cut(s)	195, 216, 1181
BspANI	GGCC	6 cut(s)	33, 54, 458, 1150, 1379, 1666
BspCNI	CTCAG	3 cut(s)	147, 400, 1603
BspFNI	CGCG	1 cut(s)	80
BspLI	GGNNCC	6 cut(s)	33, 199, 214, 570, 698, 783
BspMAI	CTGCAG	1 cut(s)	1522
BspMI	ACCTGC	1 cut(s)	283
BspPI	GGATC	5 cut(s)	563, 576, 774, 1313, 1494
BspT107I	GGYRCC	2 cut(s)	197, 212
BspTNI	GGTCTC	1 cut(s)	218
BsrDI	GCAATG	1 cut(s)	1425
BsrFI	RCCGGY	1 cut(s)	74
BsrI	ACTGG	2 cut(s)	785, 991
BssAI	RCCGGY	1 cut(s)	74
BssHII	GCGCGC	1 cut(s)	78
BssMI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
BssNI	GRCGYC	2 cut(s)	144, 344
BssSI	CACGAG	1 cut(s)	467
BssT1I	CCWWGG	5 cut(s)	24, 234, 255, 1380, 1667
Bst2BI	CACGAG	1 cut(s)	467
Bst2UI	CCWGG	1 cut(s)	763
Bst4CI	ACNGT	6 cut(s)	184, 450, 536, 753, 958, 1205
Bst6I	CTCTTC	1 cut(s)	654
BstACI	GRCGYC	2 cut(s)	144, 344
BstC8I	GCNNGC	4 cut(s)	80, 154, 1191, 1546
BstDEI	CTNAG	5 cut(s)	36, 134, 408, 731, 1611
BstDSI	CCRYGG	5 cut(s)	219, 234, 599, 1380, 1667
BstF5I	GGATG	2 cut(s)	45, 913
BstFNI	CGCG	1 cut(s)	80
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	4 cut(s)	80, 82, 140, 838
BstKTI	GATC	6 cut(s)	264, 523, 571, 769, 1321, 1489
BstMAI	GTCTC	5 cut(s)	170, 218, 1607, 1611, 1740
BstMBI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
BstMWI	GCNNNNNNNGC	3 cut(s)	135, 1195, 1307
BstNI	CCWGG	1 cut(s)	763
BstSCI	CCNGG	3 cut(s)	49, 168, 761
BstSFI	CTRYAG	1 cut(s)	1518
BstSLI	GKGCMC	2 cut(s)	35, 485
BstUI	CGCG	1 cut(s)	80
BstV1I	GCAGC	7 cut(s)	69, 113, 372, 433, 436, 1484, 1606
BstV2I	GAAGAC	2 cut(s)	139, 1016
BstX2I	RGATCY	3 cut(s)	261, 568, 766
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	3 cut(s)	261, 568, 766
BsuI	GTATCC	2 cut(s)	167, 1156
BsuRI	GGCC	6 cut(s)	33, 54, 458, 1150, 1379, 1666
BtgI	CCRYGG	5 cut(s)	219, 234, 599, 1380, 1667
BtsCI	GGATG	2 cut(s)	45, 913
BtsI	GCAGTG	1 cut(s)	1527
BtsIMutI	CAGTG	4 cut(s)	530, 687, 1210, 1527
BveI	ACCTGC	1 cut(s)	283
Cac8I	GCNNGC	4 cut(s)	80, 154, 1191, 1546
CfoI	GCGC	4 cut(s)	80, 82, 140, 838
Cfr10I	RCCGGY	1 cut(s)	74
Cfr13I	GGNCC	4 cut(s)	31, 32, 999, 1149
CseI	GACGC	2 cut(s)	352, 1624
Csp6I	GTAC	3 cut(s)	208, 274, 1679
CspCI	CAANNNNNGTGG	2 cut(s)	961, 996
CviAII	CATG	6 cut(s)	235, 794, 1176, 1381, 1564, 1668
CviQI	GTAC	3 cut(s)	208, 274, 1679
DdeI	CTNAG	5 cut(s)	36, 134, 408, 731, 1611
DpnI	GATC	6 cut(s)	263, 522, 570, 768, 1320, 1488
DpnII	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
DraI	TTTAAA	1 cut(s)	549
DraIII	CACNNNGTG	2 cut(s)	958, 1631
EaeI	YGGCCR	3 cut(s)	52, 456, 1664
Eam1104I	CTCTTC	1 cut(s)	654
EarI	CTCTTC	1 cut(s)	654
EciI	GGCGGA	1 cut(s)	1196
Eco130I	CCWWGG	5 cut(s)	24, 234, 255, 1380, 1667
Eco24I	GRGCYC	1 cut(s)	35
Eco31I	GGTCTC	1 cut(s)	218
Eco47I	GGWCC	1 cut(s)	999
Eco57I	CTGAAG	3 cut(s)	665, 787, 791
Eco88I	CYCGRG	1 cut(s)	1151
EcoO109I	RGGNCCY	2 cut(s)	31, 32
EcoRII	CCWGG	1 cut(s)	761
EcoT14I	CCWWGG	5 cut(s)	24, 234, 255, 1380, 1667
EcoT22I	ATGCAT	1 cut(s)	1447
EcoT38I	GRGCYC	1 cut(s)	35
ErhI	CCWWGG	5 cut(s)	24, 234, 255, 1380, 1667
Esp3I	CGTCTC	2 cut(s)	170, 1607
FaeI	CATG	6 cut(s)	238, 797, 1179, 1384, 1567, 1671
FaqI	GGGAC	2 cut(s)	86, 821
FatI	CATG	6 cut(s)	234, 793, 1175, 1380, 1563, 1667
FblI	GTMKAC	1 cut(s)	593
Fnu4HI	GCNGC	8 cut(s)	83, 127, 196, 386, 422, 425, 1498, 1595
FokI	GGATG	2 cut(s)	32, 920
FriOI	GRGCYC	1 cut(s)	35
Fsp4HI	GCNGC	8 cut(s)	83, 127, 196, 386, 422, 425, 1498, 1595
FspBI	CTAG	3 cut(s)	1415, 1529, 1683
GlaI	GCGC	4 cut(s)	79, 81, 139, 837
GluI	GCNGC	8 cut(s)	83, 127, 196, 386, 422, 425, 1498, 1595
GsuI	CTGGAG	2 cut(s)	410, 858
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	6 cut(s)	33, 54, 458, 1150, 1379, 1666
HapII	CCGG	4 cut(s)	51, 75, 169, 347
HgaI	GACGC	2 cut(s)	352, 1624
HhaI	GCGC	4 cut(s)	80, 82, 140, 838
Hin1I	GRCGYC	2 cut(s)	144, 344
Hin1II	CATG	6 cut(s)	238, 797, 1179, 1384, 1567, 1671
Hin6I	GCGC	4 cut(s)	78, 80, 138, 836
HinP1I	GCGC	4 cut(s)	78, 80, 138, 836
HincII	GTYRAC	2 cut(s)	94, 594
HindII	GTYRAC	2 cut(s)	94, 594
HindIII	AAGCTT	2 cut(s)	542, 1472
HinfI	GANTC	6 cut(s)	283, 595, 604, 898, 1283, 1407
HpaII	CCGG	4 cut(s)	51, 75, 169, 347
HphI	GGTGA	5 cut(s)	64, 751, 970, 1001, 1655
Hpy166II	GTNNAC	2 cut(s)	94, 594
Hpy188I	TCNGA	4 cut(s)	409, 771, 1162, 1318
Hpy188III	TCNNGA	8 cut(s)	608, 629, 875, 902, 1295, 1304, 1468, 1610
Hpy8I	GTNNAC	2 cut(s)	94, 594
Hpy99I	CGWCG	2 cut(s)	146, 346
HpyAV	CCTTC	5 cut(s)	640, 978, 1381, 1430, 1570
HpyCH4III	ACNGT	6 cut(s)	184, 450, 536, 753, 958, 1205
HpyCH4IV	ACGT	5 cut(s)	63, 90, 99, 144, 479
HpyCH4V	TGCA	9 cut(s)	421, 444, 512, 579, 1189, 1445, 1453, 1520, 1534
HpyF10VI	GCNNNNNNNGC	3 cut(s)	135, 1195, 1307
HpyF3I	CTNAG	5 cut(s)	36, 134, 408, 731, 1611
HpySE526I	ACGT	5 cut(s)	63, 90, 99, 144, 479
Hsp92I	GRCGYC	2 cut(s)	144, 344
Hsp92II	CATG	6 cut(s)	238, 797, 1179, 1384, 1567, 1671
HspAI	GCGC	4 cut(s)	78, 80, 138, 836
Kzo9I	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
LmnI	GCTCC	5 cut(s)	45, 349, 696, 1363, 1505
Lsp1109I	GCAGC	7 cut(s)	69, 113, 372, 433, 436, 1484, 1606
LweI	GCATC	5 cut(s)	823, 1081, 1288, 1297, 1543
MaeI	CTAG	3 cut(s)	1415, 1529, 1683
MaeII	ACGT	5 cut(s)	63, 90, 99, 144, 479
MaeIII	GTNAC	3 cut(s)	16, 1021, 1688
MalI	GATC	6 cut(s)	263, 522, 570, 768, 1320, 1488
MboI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
MboII	GAAGA	5 cut(s)	139, 671, 907, 1021, 1529
MflI	RGATCY	3 cut(s)	261, 568, 766
MhlI	GDGCHC	3 cut(s)	35, 485, 1748
MlyI	GAGTC	3 cut(s)	589, 1277, 1401
MmeI	TCCRAC	3 cut(s)	636, 1054, 1140
Mph1103I	ATGCAT	1 cut(s)	1447
MroXI	GAANNNNTTC	1 cut(s)	1054
MseI	TTAA	8 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599
MslI	CAYNNNNRTG	2 cut(s)	959, 1508
MspI	CCGG	4 cut(s)	51, 75, 169, 347
MspR9I	CCNGG	3 cut(s)	51, 170, 763
Mva1269I	GAATGC	2 cut(s)	709, 1447
MvaI	CCWGG	1 cut(s)	763
MvnI	CGCG	1 cut(s)	80
MwoI	GCNNNNNNNGC	3 cut(s)	135, 1195, 1307
NciI	CCSGG	2 cut(s)	51, 170
NcoI	CCATGG	3 cut(s)	234, 1380, 1667
NdeII	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
NlaIII	CATG	6 cut(s)	238, 797, 1179, 1384, 1567, 1671
NlaIV	GGNNCC	6 cut(s)	33, 199, 214, 570, 698, 783
NmeAIII	GCCGAG	1 cut(s)	90
NmuCI	GTSAC	1 cut(s)	1688
NsiI	ATGCAT	1 cut(s)	1447
PaqCI	CACCTGC	1 cut(s)	283
PauI	GCGCGC	1 cut(s)	78
PcsI	WCGNNNNNNNCGW	2 cut(s)	96, 160
PctI	GAATGC	2 cut(s)	709, 1447
PdmI	GAANNNNTTC	1 cut(s)	1054
PfeI	GAWTC	3 cut(s)	283, 604, 898
PkrI	GCNGC	8 cut(s)	84, 128, 197, 387, 423, 426, 1499, 1596
PleI	GAGTC	3 cut(s)	589, 1277, 1401
PpsI	GAGTC	3 cut(s)	589, 1277, 1401
PshBI	ATTAAT	1 cut(s)	669
Psp6I	CCWGG	1 cut(s)	761
PspGI	CCWGG	1 cut(s)	761
PspN4I	GGNNCC	6 cut(s)	33, 199, 214, 570, 698, 783
PspOMI	GGGCCC	1 cut(s)	31
PspPI	GGNCC	4 cut(s)	31, 32, 999, 1149
PsrI	GAACNNNNNNTAC	2 cut(s)	290, 322
PstI	CTGCAG	1 cut(s)	1522
PsuI	RGATCY	3 cut(s)	261, 568, 766
PteI	GCGCGC	1 cut(s)	78
RsaI	GTAC	3 cut(s)	209, 275, 1680
RsaNI	GTAC	3 cut(s)	208, 274, 1679
RseI	CAYNNNNRTG	2 cut(s)	959, 1508
SalI	GTCGAC	1 cut(s)	592
SaqAI	TTAA	8 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599
SatI	GCNGC	8 cut(s)	83, 127, 196, 386, 422, 425, 1498, 1595
Sau3AI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
Sau96I	GGNCC	4 cut(s)	31, 32, 999, 1149
ScaI	AGTACT	1 cut(s)	275
SchI	GAGTC	3 cut(s)	589, 1277, 1401
ScrFI	CCNGG	3 cut(s)	51, 170, 763
SduI	GDGCHC	3 cut(s)	35, 485, 1748
SfaNI	GCATC	5 cut(s)	823, 1081, 1288, 1297, 1543
SfcI	CTRYAG	1 cut(s)	1518
SgrAI	CRCCGGYG	1 cut(s)	74
SinI	GGWCC	1 cut(s)	999
SmiMI	CAYNNNNRTG	2 cut(s)	959, 1508
SmlI	CTYRAG	1 cut(s)	1302
SmoI	CTYRAG	1 cut(s)	1302
SsiI	CCGC	3 cut(s)	195, 216, 1181
SspI	AATATT	1 cut(s)	313
SspMI	CTAG	3 cut(s)	1415, 1529, 1683
StyD4I	CCNGG	3 cut(s)	49, 168, 761
StyI	CCWWGG	5 cut(s)	24, 234, 255, 1380, 1667
TaaI	ACNGT	6 cut(s)	184, 450, 536, 753, 958, 1205
TaiI	ACGT	5 cut(s)	66, 93, 102, 147, 482
TaqI	TCGA	2 cut(s)	341, 593
TatI	WGTACW	1 cut(s)	273
TauI	GCSGC	1 cut(s)	198
TfiI	GAWTC	3 cut(s)	283, 604, 898
Tru1I	TTAA	8 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599
Tru9I	TTAA	8 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599
TscAI	CASTG	4 cut(s)	530, 687, 1210, 1527
TseFI	GTSAC	1 cut(s)	1688
TseI	GCWGC	7 cut(s)	82, 126, 385, 421, 424, 1497, 1594
Tsp45I	GTSAC	1 cut(s)	1688
TspDTI	ATGAA	6 cut(s)	275, 701, 1022, 1056, 1222, 1552
TspGWI	ACGGA	3 cut(s)	236, 371, 616
TspRI	CASTG	4 cut(s)	530, 687, 1210, 1527
VpaK11BI	GGWCC	1 cut(s)	999
VspI	ATTAAT	1 cut(s)	669
XapI	RAATTY	1 cut(s)	326
XmiI	GTMKAC	1 cut(s)	593
XmnI	GAANNNNTTC	1 cut(s)	1054
XspI	CTAG	3 cut(s)	1415, 1529, 1683
ZraI	GACGTC	1 cut(s)	145
ZrmI	AGTACT	1 cut(s)	275
Zsp2I	ATGCAT	1 cut(s)	1447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.