Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Reverse (-)

82456991 .. 82458787

Gene Structure 1797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG593100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGGGAGACCTCGCCAAGCAGGACCAGAGCGTCCCCAAGCTCCCCATCCCGGGGGAGCGAAACATCCTGATCACTAGTGCCTTGCCATATGTTAACAACGTCCCCCACCTCGGGAACATCATTGGCTGCGTCCTGAGCGCCGATGTCTTTGCTCGGTACTGCCGTCTCCGGGGATACAATACCGTCTATATCTCCGGGACCGATGAGTATGGCACAGCGACGGAGACCAAAGCCATGGAAGAGAAGTGCAGCCCCCAAGAGATATGTGACAAGTATCACAAAATCCATAGGGAGGTTTATGAGTGGTTCAACATAAGCTTCGACCAATTCGGTCGAACTTCCACGCCGCAGCAAACTGAGGTCTGCCAACAAATCTTCAAGAAATTGTTGGACAGTGATCGCCTTGCTGAAAACACAATCCAGCAGCTCTATTGTGACTCATGCAAAAGATTCTTAGCGGACCGATTCGTGGAGGGGACATGCCCTACTCAAGGATGTTACTACGATTCTGCTCGAGGAGACCAATGTGACAAGTGTGGAAAGCTTTTGAATCCCACGGAACTGAAAGATCCTAAATGCAAGGTATGCAAGGAAACTCCACGGATTCGCAAAACAAATCACTTGTTTCTGGAGCTCCCTTTGCTCGAGGATAAGTTGGAAAACTACATCAATAAGGCATCAGTGGAAGGGTGTTGGAGTCAGAATGCGGTTCAAATCACAAATGCCTGGCTCAAAGAGGGGCTTAAACAACGTTGCATTACAAGGGATCTCAAGTGGGGTGTTCCGGTTCCACACGATGGCTTCAGGGACAAGGTATTCTACGTGTGGTTCGATGCTCCTATTGGATACATCTCCATTACTAAGTGTCACACTCCTGATCACTGGCAGAAGTGGTGGAAGAATCCTGAGGATGTCGAATTGTATCAGTTTATGGGCAAGGACAATGTGCCATTTCACACGGTCATGTTTCCTTCCGCGCTTCTTGGAACCGGAGAAAATTGGACACTCATGAAGAACATTAGTGTCACTGAATACTTGAACTATGAAGAAGGCAAGTTTTCCAAGAGTAGAGGCATTGGAGTTTTTGGAAACGATGCAAAAGGAACGGGCATTCCAGTTGAAGTTTGGCGTTATTACCTGCTGACTAATAGGCCTGAGGTATCAGACACATTGTTCACCTGGGCCGATTTACAAGCAAAACTCAAAAGTGAGTTGGTGGGCAATTTGGGCAACTTCATCAACAGAGTTTTGAGTTTCGTTGCCAAACCTTCAGCCAAAGGAGGATACGGTGACACCATTCCTGATGCTCCACATGCAGACTCTGATGCCTTGACACTTGAATTAGCAGAAATAGTCGGTAGATACGTCGAGCAATACATAGAAGCAATGGAGAAGCTCAAACTAAAGCAAGGGCTGAAAATAGCCATGAGCATTTCTAGCGAGGGGAACGCATATCTCCAAGAATCCAAGTTCTGGAAGCTCTACAAGGAGGACCGACCTCGGTGCTCCATTGTCATTAAAACTTCAGTAGGGTTAGTGCATCTTCTTGCTTGCTTATTGGAACCTTACATGCCCTCATTTTCTGCTAGAGTACTTGAGCAGCTAAACTTGCCTCCTGAGTCAATCAAATCCCTTTGTGATGTCAATAAAGCAAAACGGCCTTGGGAGATCGTACCTGATGGTCACAAGATTGGAGAACCCAAGACATTGTTCACGACGTTGAACGGCGAAGAAGTCGAATCTCTGCGGAAAAAGTTTTCTGGAAGCCAAGCTGATAGGGAAAAACGTACACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g tRNA_bind

Protein Analysis

598

Amino Acids

68.11

Weight (kDa)

7.11

Isoelectric Point (pI)

34.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	22 - 417	1.2e-155	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	24 - 265	2.7e-09	tRNA synthetases class I (I, L, M and V)
Anticodon_3	PF19303	447 - 590	1.2e-18	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	29
Acc36I	ACCTGC	1 cut(s)	1146
AccB7I	CCANNNNNTGG	2 cut(s)	797, 1473
AccII	CGCG	1 cut(s)	977
AciI	CCGC	5 cut(s)	347, 458, 707, 975, 1747
AclI	AACGTT	1 cut(s)	751
AclWI	GGATC	2 cut(s)	563, 774
AcuI	CTGAAG	3 cut(s)	787, 1254, 1509
AfaI	GTAC	4 cut(s)	158, 1593, 1674, 1789
AflIII	ACRYGT	1 cut(s)	822
AgsI	TTSAA	8 cut(s)	310, 379, 550, 713, 1039, 1121, 1340, 1723
AhlI	ACTAGT	1 cut(s)	74
AjnI	CCWGG	2 cut(s)	725, 1178
AjuI	GAANNNNNNNTTGG	2 cut(s)	1694, 1726
AloI	GAACNNNNNNTCC	2 cut(s)	1096, 1128
AluBI	AGCT	9 cut(s)	40, 318, 427, 544, 634, 1396, 1480, 1603, 1772
AluI	AGCT	9 cut(s)	40, 318, 427, 544, 634, 1396, 1480, 1603, 1772
Alw21I	GWGCWC	2 cut(s)	636, 1508
Alw26I	GTCTC	3 cut(s)	170, 218, 513
AlwI	GGATC	2 cut(s)	563, 774
AlwNI	CAGNNNCTG	1 cut(s)	1322
Ama87I	CYCGRG	4 cut(s)	49, 110, 513, 644
AoxI	GGCC	3 cut(s)	1151, 1182, 1658
ApeKI	GCWGC	5 cut(s)	126, 249, 349, 424, 1600
AspLEI	GCGC	2 cut(s)	140, 979
AspS9I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1492
AsuC2I	CCSGG	4 cut(s)	50, 51, 170, 196
AsuHPI	GGTGA	2 cut(s)	1168, 1301
AvaI	CYCGRG	4 cut(s)	49, 110, 513, 644
AvaII	GGWCC	4 cut(s)	22, 198, 460, 1492
AxyI	CCTNAGG	2 cut(s)	906, 1155
BaeI	ACNNNNGTAYC	2 cut(s)	148, 181
BanII	GRGCYC	1 cut(s)	636
Bbv12I	GWGCWC	2 cut(s)	636, 1508
BbvI	GCAGC	5 cut(s)	113, 261, 361, 436, 1612
BccI	CCATC	3 cut(s)	53, 791, 1673
BceAI	ACGGC	3 cut(s)	147, 1673, 1741
BcgI	CGANNNNNNTGC	4 cut(s)	131, 165, 1485, 1519
BciT130I	CCWGG	2 cut(s)	727, 1180
BciVI	GTATCC	3 cut(s)	167, 839, 1277
BclI	TGATCA	2 cut(s)	69, 877
BcnI	CCSGG	4 cut(s)	50, 51, 170, 196
BcoDI	GTCTC	3 cut(s)	170, 218, 513
BcuI	ACTAGT	1 cut(s)	74
BfaI	CTAG	3 cut(s)	75, 1437, 1587
BfoI	RGCGCY	1 cut(s)	141
BfuAI	ACCTGC	1 cut(s)	1146
BfuI	GTATCC	3 cut(s)	167, 839, 1277
BisI	GCNGC	6 cut(s)	127, 250, 347, 350, 425, 1601
BlsI	GCNGC	6 cut(s)	128, 251, 348, 351, 426, 1602
BmcAI	AGTACT	1 cut(s)	1593
Bme1390I	CCNGG	6 cut(s)	50, 51, 170, 196, 727, 1180
Bme18I	GGWCC	4 cut(s)	22, 198, 460, 1492
BmeT110I	CYCGRG	4 cut(s)	49, 110, 513, 644
BmgT120I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1492
BmiI	GGNNCC	4 cut(s)	199, 789, 988, 1563
BmrFI	CCNGG	6 cut(s)	50, 51, 170, 196, 727, 1180
BmsI	GCATC	6 cut(s)	686, 823, 1084, 1294, 1315, 1549
BpmI	CTGGAG	1 cut(s)	650
Bpu10I	CCTNAGC	1 cut(s)	134
BpuEI	CTTGAG	3 cut(s)	474, 755, 1616
BpuMI	CCSGG	4 cut(s)	50, 51, 170, 196
BsaAI	YACGTR	1 cut(s)	823
BsaI	GGTCTC	2 cut(s)	218, 513
BsaWI	WCCGGW	2 cut(s)	784, 989
Bse1I	ACTGG	2 cut(s)	887, 1115
Bse21I	CCTNAGG	2 cut(s)	906, 1155
Bse3DI	GCAATG	1 cut(s)	1392
BseBI	CCWGG	2 cut(s)	727, 1180
BseGI	GGATG	4 cut(s)	45, 63, 500, 916
BseMI	GCAATG	1 cut(s)	1392
BseMII	CTCAG	5 cut(s)	125, 348, 897, 1146, 1608
BseNI	ACTGG	2 cut(s)	887, 1115
BseRI	GAGGAG	1 cut(s)	531
BseXI	GCAGC	5 cut(s)	113, 261, 361, 436, 1612
BsgI	GTGCAG	1 cut(s)	268
Bsh1236I	CGCG	1 cut(s)	977
Bsh1285I	CGRYCG	1 cut(s)	334
BshFI	GGCC	3 cut(s)	1153, 1184, 1660
BsiEI	CGRYCG	1 cut(s)	334
BsiHKAI	GWGCWC	2 cut(s)	636, 1508
BsiHKCI	CYCGRG	4 cut(s)	49, 110, 513, 644
BsiSI	CCGG	5 cut(s)	50, 169, 195, 785, 990
BslFI	GGGAC	5 cut(s)	17, 86, 211, 490, 821
BsmAI	GTCTC	3 cut(s)	170, 218, 513
BsmBI	CGTCTC	1 cut(s)	170
BsmFI	GGGAC	5 cut(s)	17, 86, 211, 490, 821
BsmI	GAATGC	2 cut(s)	709, 1110
BsnI	GGCC	3 cut(s)	1153, 1184, 1660
Bso31I	GGTCTC	2 cut(s)	218, 513
BsoBI	CYCGRG	4 cut(s)	49, 110, 513, 644
Bsp1286I	GDGCHC	2 cut(s)	636, 1508
Bsp143I	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
Bsp19I	CCATGG	1 cut(s)	234
BspACI	CCGC	5 cut(s)	347, 458, 707, 975, 1747
BspANI	GGCC	3 cut(s)	1153, 1184, 1660
BspCNI	CTCAG	5 cut(s)	126, 349, 898, 1147, 1609
BspFNI	CGCG	1 cut(s)	977
BspHI	TCATGA	1 cut(s)	1008
BspLI	GGNNCC	4 cut(s)	199, 789, 988, 1563
BspMI	ACCTGC	1 cut(s)	1146
BspPI	GGATC	2 cut(s)	563, 774
BspTNI	GGTCTC	2 cut(s)	218, 513
BsrDI	GCAATG	1 cut(s)	1392
BsrI	ACTGG	2 cut(s)	887, 1115
BssMI	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
BssT1I	CCWWGG	2 cut(s)	234, 1661
Bst2UI	CCWGG	2 cut(s)	727, 1180
Bst4CI	ACNGT	4 cut(s)	184, 395, 961, 1289
Bst6I	CTCTTC	1 cut(s)	234
BstAPI	GCANNNNNTGC	1 cut(s)	585
BstBAI	YACGTR	1 cut(s)	823
BstC8I	GCNNGC	1 cut(s)	1552
BstDEI	CTNAG	7 cut(s)	134, 357, 454, 861, 906, 1155, 1617
BstDSI	CCRYGG	3 cut(s)	234, 555, 599
BstENI	CCTNNNNNAGG	1 cut(s)	489
BstF5I	GGATG	4 cut(s)	45, 63, 500, 916
BstFNI	CGCG	1 cut(s)	977
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	2 cut(s)	140, 979
BstKTI	GATC	6 cut(s)	72, 400, 571, 769, 880, 1671
BstMAI	GTCTC	3 cut(s)	170, 218, 513
BstMBI	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
BstMCI	CGRYCG	1 cut(s)	334
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 585, 640, 1227, 1313, 1437, 1609
BstNI	CCWGG	2 cut(s)	727, 1180
BstNSI	RCATGY	3 cut(s)	483, 1316, 1573
BstSCI	CCNGG	6 cut(s)	48, 49, 168, 194, 725, 1178
BstUI	CGCG	1 cut(s)	977
BstV1I	GCAGC	5 cut(s)	113, 261, 361, 436, 1612
BstX2I	RGATCY	2 cut(s)	568, 766
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	2 cut(s)	568, 766
Bsu36I	CCTNAGG	2 cut(s)	906, 1155
BsuI	GTATCC	3 cut(s)	167, 839, 1277
BsuRI	GGCC	3 cut(s)	1153, 1184, 1660
BtgI	CCRYGG	3 cut(s)	234, 555, 599
BtsCI	GGATG	4 cut(s)	45, 63, 500, 916
BtsIMutI	CAGTG	4 cut(s)	400, 687, 880, 1026
BveI	ACCTGC	1 cut(s)	1146
Cac8I	GCNNGC	1 cut(s)	1552
CaiI	CAGNNNCTG	1 cut(s)	1322
CciI	TCATGA	1 cut(s)	1008
CfoI	GCGC	2 cut(s)	140, 979
Cfr13I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1492
Cfr9I	CCCGGG	1 cut(s)	49
CpoI	CGGWCCG	1 cut(s)	460
CseI	GACGC	2 cut(s)	19, 118
Csp6I	GTAC	4 cut(s)	157, 1592, 1673, 1788
CspI	CGGWCCG	1 cut(s)	460
CviAII	CATG	8 cut(s)	235, 441, 480, 964, 1009, 1313, 1426, 1570
CviQI	GTAC	4 cut(s)	157, 1592, 1673, 1788
DdeI	CTNAG	7 cut(s)	134, 357, 454, 861, 906, 1155, 1617
DpnI	GATC	6 cut(s)	71, 399, 570, 768, 879, 1670
DpnII	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
DrdI	GACNNNNNNGTC	1 cut(s)	29
DseDI	GACNNNNNNGTC	1 cut(s)	29
Eam1104I	CTCTTC	1 cut(s)	234
EarI	CTCTTC	1 cut(s)	234
Ecl136II	GAGCTC	1 cut(s)	634
Eco130I	CCWWGG	2 cut(s)	234, 1661
Eco147I	AGGCCT	1 cut(s)	1153
Eco24I	GRGCYC	1 cut(s)	636
Eco31I	GGTCTC	2 cut(s)	218, 513
Eco47I	GGWCC	4 cut(s)	22, 198, 460, 1492
Eco53kI	GAGCTC	1 cut(s)	634
Eco57I	CTGAAG	3 cut(s)	787, 1254, 1509
Eco81I	CCTNAGG	2 cut(s)	906, 1155
Eco88I	CYCGRG	4 cut(s)	49, 110, 513, 644
EcoICRI	GAGCTC	1 cut(s)	634
EcoNI	CCTNNNNNAGG	1 cut(s)	489
EcoRII	CCWGG	2 cut(s)	725, 1178
EcoT14I	CCWWGG	2 cut(s)	234, 1661
EcoT38I	GRGCYC	1 cut(s)	636
ErhI	CCWWGG	2 cut(s)	234, 1661
Esp3I	CGTCTC	1 cut(s)	170
FaeI	CATG	8 cut(s)	238, 444, 483, 967, 1012, 1316, 1429, 1573
FalI	AAGNNNNNCTT	2 cut(s)	726, 758
FaqI	GGGAC	5 cut(s)	17, 86, 211, 490, 821
FatI	CATG	8 cut(s)	234, 440, 479, 963, 1008, 1312, 1425, 1569
FauNDI	CATATG	1 cut(s)	88
FbaI	TGATCA	2 cut(s)	69, 877
Fnu4HI	GCNGC	6 cut(s)	127, 250, 347, 350, 425, 1601
FokI	GGATG	4 cut(s)	32, 50, 507, 923
FriOI	GRGCYC	1 cut(s)	636
Fsp4HI	GCNGC	6 cut(s)	127, 250, 347, 350, 425, 1601
FspBI	CTAG	3 cut(s)	75, 1437, 1587
GlaI	GCGC	2 cut(s)	139, 978
GluI	GCNGC	6 cut(s)	127, 250, 347, 350, 425, 1601
GsuI	CTGGAG	1 cut(s)	650
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	3 cut(s)	1153, 1184, 1660
HapII	CCGG	5 cut(s)	50, 169, 195, 785, 990
HgaI	GACGC	2 cut(s)	19, 118
HhaI	GCGC	2 cut(s)	140, 979
Hin1II	CATG	8 cut(s)	238, 444, 483, 967, 1012, 1316, 1429, 1573
Hin6I	GCGC	2 cut(s)	138, 977
HinP1I	GCGC	2 cut(s)	138, 977
HincII	GTYRAC	1 cut(s)	94
HindII	GTYRAC	1 cut(s)	94
HindIII	AAGCTT	2 cut(s)	316, 542
HpaI	GTTAAC	1 cut(s)	94
HpaII	CCGG	5 cut(s)	50, 169, 195, 785, 990
HphI	GGTGA	2 cut(s)	1168, 1301
Hpy166II	GTNNAC	4 cut(s)	94, 1176, 1713, 1790
Hpy188I	TCNGA	3 cut(s)	702, 1165, 1324
Hpy8I	GTNNAC	4 cut(s)	94, 1176, 1713, 1790
Hpy99I	CGWCG	3 cut(s)	223, 1370, 1720
HpyAV	CCTTC	4 cut(s)	680, 981, 1043, 1278
HpyCH4III	ACNGT	4 cut(s)	184, 395, 961, 1289
HpyCH4IV	ACGT	6 cut(s)	99, 751, 822, 1365, 1718, 1786
HpyCH4V	TGCA	8 cut(s)	249, 444, 579, 588, 756, 1097, 1316, 1540
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 585, 640, 1227, 1313, 1437, 1609
HpyF3I	CTNAG	7 cut(s)	134, 357, 454, 861, 906, 1155, 1617
HpySE526I	ACGT	6 cut(s)	99, 751, 822, 1365, 1718, 1786
Hsp92II	CATG	8 cut(s)	238, 444, 483, 967, 1012, 1316, 1429, 1573
HspAI	GCGC	2 cut(s)	138, 977
Ksp22I	TGATCA	2 cut(s)	69, 877
KspAI	GTTAAC	1 cut(s)	94
Kzo9I	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
LmnI	GCTCC	7 cut(s)	45, 55, 631, 639, 841, 1312, 1511
Lsp1109I	GCAGC	5 cut(s)	113, 261, 361, 436, 1612
LweI	GCATC	6 cut(s)	686, 823, 1084, 1294, 1315, 1549
MaeI	CTAG	3 cut(s)	75, 1437, 1587
MaeII	ACGT	6 cut(s)	99, 751, 822, 1365, 1718, 1786
MaeIII	GTNAC	8 cut(s)	266, 434, 497, 527, 866, 1024, 1289, 1682
MalI	GATC	6 cut(s)	71, 399, 570, 768, 879, 1670
MboI	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
MboII	GAAGA	7 cut(s)	251, 367, 910, 1024, 1058, 1535, 1742
MflI	RGATCY	2 cut(s)	568, 766
MhlI	GDGCHC	2 cut(s)	636, 1508
MluCI	AATT	6 cut(s)	326, 383, 917, 997, 1222, 1340
MlyI	GAGTC	4 cut(s)	431, 706, 1313, 1628
MmeI	TCCRAC	3 cut(s)	369, 636, 674
MseI	TTAA	3 cut(s)	93, 744, 1518
MslI	CAYNNNNRTG	1 cut(s)	962
MspI	CCGG	5 cut(s)	50, 169, 195, 785, 990
MspR9I	CCNGG	6 cut(s)	50, 51, 170, 196, 727, 1180
Mva1269I	GAATGC	2 cut(s)	709, 1110
MvaI	CCWGG	2 cut(s)	727, 1180
MvnI	CGCG	1 cut(s)	977
MwoI	GCNNNNNNNGC	7 cut(s)	135, 585, 640, 1227, 1313, 1437, 1609
NciI	CCSGG	4 cut(s)	50, 51, 170, 196
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	88
NdeII	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
NlaIII	CATG	8 cut(s)	238, 444, 483, 967, 1012, 1316, 1429, 1573
NlaIV	GGNNCC	4 cut(s)	199, 789, 988, 1563
NmuCI	GTSAC	7 cut(s)	266, 434, 527, 866, 1024, 1289, 1682
NspI	RCATGY	3 cut(s)	483, 1316, 1573
PaeR7I	CTCGAG	2 cut(s)	513, 644
PagI	TCATGA	1 cut(s)	1008
PceI	AGGCCT	1 cut(s)	1153
PcsI	WCGNNNNNNNCGW	3 cut(s)	160, 828, 1362
PctI	GAATGC	2 cut(s)	709, 1110
PfeI	GAWTC	8 cut(s)	450, 465, 506, 550, 604, 901, 1463, 1739
PflMI	CCANNNNNTGG	2 cut(s)	797, 1473
PfoI	TCCNGGA	1 cut(s)	194
PkrI	GCNGC	6 cut(s)	128, 251, 348, 351, 426, 1602
PleI	GAGTC	4 cut(s)	431, 705, 1313, 1627
PpsI	GAGTC	4 cut(s)	431, 705, 1313, 1627
Ppu21I	YACGTR	1 cut(s)	823
Psp124BI	GAGCTC	1 cut(s)	636
Psp1406I	AACGTT	1 cut(s)	751
Psp6I	CCWGG	2 cut(s)	725, 1178
PspGI	CCWGG	2 cut(s)	725, 1178
PspN4I	GGNNCC	4 cut(s)	199, 789, 988, 1563
PspPI	GGNCC	5 cut(s)	22, 198, 460, 1182, 1492
PspXI	VCTCGAGB	2 cut(s)	513, 644
PstNI	CAGNNNCTG	1 cut(s)	1322
PsuI	RGATCY	2 cut(s)	568, 766
RsaI	GTAC	4 cut(s)	158, 1593, 1674, 1789
RsaNI	GTAC	4 cut(s)	157, 1592, 1673, 1788
RseI	CAYNNNNRTG	1 cut(s)	962
Rsr2I	CGGWCCG	1 cut(s)	460
RsrII	CGGWCCG	1 cut(s)	460
SacI	GAGCTC	1 cut(s)	636
SaqAI	TTAA	3 cut(s)	93, 744, 1518
SatI	GCNGC	6 cut(s)	127, 250, 347, 350, 425, 1601
Sau3AI	GATC	6 cut(s)	69, 397, 568, 766, 877, 1668
Sau96I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1492
ScaI	AGTACT	1 cut(s)	1593
SchI	GAGTC	4 cut(s)	431, 706, 1313, 1628
ScrFI	CCNGG	6 cut(s)	50, 51, 170, 196, 727, 1180
SduI	GDGCHC	2 cut(s)	636, 1508
SfaNI	GCATC	6 cut(s)	686, 823, 1084, 1294, 1315, 1549
Sfr274I	CTCGAG	2 cut(s)	513, 644
SinI	GGWCC	4 cut(s)	22, 198, 460, 1492
SlaI	CTCGAG	2 cut(s)	513, 644
SmaI	CCCGGG	1 cut(s)	51
SmiMI	CAYNNNNRTG	1 cut(s)	962
SmlI	CTYRAG	5 cut(s)	489, 513, 644, 770, 1595
SmoI	CTYRAG	5 cut(s)	489, 513, 644, 770, 1595
SpeI	ACTAGT	1 cut(s)	74
Sse9I	AATT	6 cut(s)	326, 383, 917, 997, 1222, 1340
SseBI	AGGCCT	1 cut(s)	1153
SsiI	CCGC	5 cut(s)	347, 458, 707, 975, 1747
SspMI	CTAG	3 cut(s)	75, 1437, 1587
SstI	GAGCTC	1 cut(s)	636
StuI	AGGCCT	1 cut(s)	1153
StyD4I	CCNGG	6 cut(s)	48, 49, 168, 194, 725, 1178
StyI	CCWWGG	2 cut(s)	234, 1661
TaaI	ACNGT	4 cut(s)	184, 395, 961, 1289
TaiI	ACGT	6 cut(s)	102, 754, 825, 1368, 1721, 1789
TaqI	TCGA	8 cut(s)	321, 334, 514, 645, 831, 915, 1368, 1737
TaqII	GACCGA	4 cut(s)	215, 320, 477, 1509
TasI	AATT	6 cut(s)	326, 383, 917, 997, 1222, 1340
TatI	WGTACW	1 cut(s)	1591
TauI	GCSGC	1 cut(s)	349
TfiI	GAWTC	8 cut(s)	450, 465, 506, 550, 604, 901, 1463, 1739
Tru1I	TTAA	3 cut(s)	93, 744, 1518
Tru9I	TTAA	3 cut(s)	93, 744, 1518
TscAI	CASTG	4 cut(s)	400, 687, 887, 1033
TseFI	GTSAC	7 cut(s)	266, 434, 527, 866, 1024, 1289, 1682
TseI	GCWGC	5 cut(s)	126, 249, 349, 424, 1600
Tsp45I	GTSAC	7 cut(s)	266, 434, 527, 866, 1024, 1289, 1682
TspDTI	ATGAA	3 cut(s)	1025, 1059, 1225
TspGWI	ACGGA	3 cut(s)	236, 572, 616
TspMI	CCCGGG	1 cut(s)	49
TspRI	CASTG	4 cut(s)	400, 687, 887, 1033
Van91I	CCANNNNNTGG	2 cut(s)	797, 1473
VpaK11BI	GGWCC	4 cut(s)	22, 198, 460, 1492
XagI	CCTNNNNNAGG	1 cut(s)	489
XceI	RCATGY	3 cut(s)	483, 1316, 1573
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1122
XhoI	CTCGAG	2 cut(s)	513, 644
XmaI	CCCGGG	1 cut(s)	49
XspI	CTAG	3 cut(s)	75, 1437, 1587
ZrmI	AGTACT	1 cut(s)	1593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.