Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Forward (+)

45997829 .. 46005062

Gene Structure 7234 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG184800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGGGAGACCTCGCCGTTACTTCCAAGGAGGGCCCCAAGCTCCCCATCCCCGGCCAACGCAACGTACTCATCACCAGCGCGCTGCCGTACGTCAACAACGTCCCTCATCTCGGCAACATCATCGGCTGCGTTCTCAGCGCCGACGTCTTCGCTCGCTACTGCCGTCTCCGGGGATACAACACCGTCTATATCTGTGGCACCGATGAGTATGGCACCGCCACGGAGACCAAAGCCCTGGAGGAGAAGTGTAGCCCCAAGGAGATCTGCGATAAATACTATGCGATTCATCGGCAGGTGTATGAGTGGTTCAATATTAGTTTTGACAAATTCGGGCGAACTTCGACGCCGGAGCAGACGGAGGTGTGCCAAGCCATTTTCAATAGGCTGCTGGAGAATAATTGGCTTTCTGAGAACACAATGCAGCAGCTATATTGTGATACTTGCACACGGTTTTTAGCCGACAGGCTCGTGGAAGGGACATGCCCAACCCAAGGTTGTGATTATACTTCTGCAAGAGGAGATCAGTGTGAGAACTGTGGGAAGCTTTTAAACCCCACAGAATTAAAGGATCCAAAGTGCAAGGTCTGTAAGTCGACTCCACGGATTCGTGATACAAACCATTTGTTTCTGGAACTGCCTTTGCTGAAGGATAGGTTGGAAGAGTATATTAATAAAATGTCAGTGGAGGGGTCTTGGAGCCAGAATGCCATTCAAGCAACAAATGCGTGGCTCAGAGAGGGGCTTAAACAACGGTGTATCACCAGGGATCTGAAGTGGGGGGTTCCAGTTCCACATGATAACTTCAGGGACAAGGTTTTCTATGTGTGGTTTGATGCGCCTATTGGATATGTTTCAATTACTAAATGCTACACTCCAGATTGGGAGAAATGGTGGAAGAATCCAGAGGATGTGGAGTTGTATCAGTTTATGGGCAAAGATAATGTGCCATTCCACACGGTGATGTTTCCTTCCACGCTTCTTGGAACTAGTGAAAATTGGACCTTAATGAAGACAATTAGTGTTACTGAATACTTGAACTATGAAGCAGGAAAGTTTTCCAAGAGTAAAGGGGTTGGAGTATTTGGAAACGATGCCAAAGATACAAACATTCCTGCAGAAGTTTGGCGATATTACTTGCTGACCAATAGGCCCGAGGTGTCGGATACATTATTTACATGGGCGGATTTGCAAGCGAAGCTCAACAGTGAATTGCTGAATAATTTGGGCAACTTCATTAACCGAGTTTTGAGTTTTGTTGCCAAACCTTCAGGTCAAGGATATGACTCCATTATTCCAGATGCTCAAGATGCCATTTCTGATCCTTTGACAGAGAAAATAGCTGAAAAAGTGGGTAAATGTGTGGAGCAATATGTGGAGGCCATGGAGAAGGTAAAACTAAAGCAAGGACTCAAACTAGCAATGAGCATTTCAAGTGAAGGGAATGCATATCTGCAAGAAAGTCAGTTCTGGAAGCTTTACAAGGAAGATCAACCTCGCTGCTCCATTGTTATGAGAACTGCAGTGGGGCTAGTGCATCTTCTTGCTTGCATATTAGAACCCTTCATGCCATCTTTTTCTGTTGAGGTATTTAAGCAGCTTAATTTGTCTCCTGAGACGCATATATCACTTTGTGATGGTAAAGGCGATACTGATAGGGCAAGACGGCCATGGGAGATTGTACCTAGAGGTCACAAGATTGGGAAACCAGAGCCGTTGTTCAAGGAATTGAATGATGATGAAGTAGATTCTCTAAGGAAGAGGTTTGCTGGAAGTCAGGCGGATAGAAAGGAAAGGGAAGAAGCTGAAGCAGTGAAGTTGGCTGAGCAGCTCAAGAAATCAAAAGTTTCAGACAAGAGTGGAAAGAAGAAACAACAAGCCACAAAATCTGCAACTGAAGCAGAGATTTCCATATCTAGACTAGATATTCGTGTCGGCCTCATTACAAAAGCTCAGAAGCATCCTGATGCTGATGCACTTTATGTGGAAGAAATTGTTGTTGGCGAAGGACAGCCTAGAACAGTTGTCAGTGGACTAGTCAGGTTTATACCTCTTGAAGAAATGCAGAATCGGAAGGTATGCGTCCTTTGCAACCTGAAGCCAGCGACCATGCGGGGTATTAAATCACAAGCGATGGTTCTTGCTGCTTCAAATAGTGACCACACCGCGGTAAGCAGCCCCATAAACCTTATAAGTAGTGTCTGCATTATTTGTTTCCATGGCTTTACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g tRNA_bind

Protein Analysis

742

Amino Acids

83.84

Weight (kDa)

6.84

Isoelectric Point (pI)

28.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	22 - 416	1.2e-154	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	24 - 265	1.4e-08	tRNA synthetases class I (I, L, M and V)
Anticodon_3	PF19303	443 - 592	8.6e-20	Anticodon binding domain of methionyl tRNA ligase
tRNA_bind	PF01588	642 - 720	1.9e-27	Putative tRNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2189
AarI	CACCTGC	1 cut(s)	283
AatII	GACGTC	1 cut(s)	147
Acc36I	ACCTGC	1 cut(s)	283
AccB1I	GGYRCC	2 cut(s)	197, 212
AccI	GTMKAC	1 cut(s)	593
AccII	CGCG	2 cut(s)	80, 2165
AciI	CCGC	6 cut(s)	216, 1181, 1778, 2110, 2163, 2165
AclWI	GGATC	4 cut(s)	563, 576, 774, 1313
AcoI	YGGCCR	2 cut(s)	52, 1664
AcsI	RAATTY	1 cut(s)	326
AcuI	CTGAAG	7 cut(s)	665, 787, 791, 1251, 1824, 1914, 2114
AcyI	GRCGYC	2 cut(s)	144, 344
AdeI	CACNNNGTG	2 cut(s)	958, 1631
AfaI	GTAC	3 cut(s)	66, 89, 1680
AfiI	CCNNNNNNNGG	5 cut(s)	50, 110, 169, 491, 2164
AhlI	ACTAGT	2 cut(s)	986, 2032
AjnI	CCWGG	2 cut(s)	234, 761
AjuI	GAANNNNNNNTTGG	4 cut(s)	638, 670, 1980, 2012
Alw26I	GTCTC	4 cut(s)	170, 218, 1607, 1611
AlwI	GGATC	4 cut(s)	563, 576, 774, 1313
Ama87I	CYCGRG	1 cut(s)	1151
AoxI	GGCC	6 cut(s)	31, 52, 1148, 1377, 1664, 1933
ApaI	GGGCCC	1 cut(s)	35
ApoI	RAATTY	1 cut(s)	326
ArsI	GACNNNNNNTTYG	2 cut(s)	1908, 1940
AseI	ATTAAT	1 cut(s)	669
Asp700I	GAANNNNTTC	1 cut(s)	1054
AspLEI	GCGC	4 cut(s)	80, 82, 140, 838
AspS9I	GGNCC	4 cut(s)	31, 32, 999, 1149
AsuC2I	CCSGG	2 cut(s)	51, 170
AsuHPI	GGTGA	3 cut(s)	64, 751, 970
AvaI	CYCGRG	1 cut(s)	1151
AvaII	GGWCC	1 cut(s)	999
BaeGI	GKGCMC	1 cut(s)	35
BamHI	GGATCC	1 cut(s)	568
BanI	GGYRCC	2 cut(s)	197, 212
BanII	GRGCYC	1 cut(s)	35
BauI	CACGAG	1 cut(s)	467
BbsI	GAAGAC	2 cut(s)	139, 1016
BccI	CCATC	4 cut(s)	53, 1576, 1628, 2125
BceAI	ACGGC	4 cut(s)	70, 147, 1679, 1696
BcgI	CGANNNNNNTGC	2 cut(s)	103, 137
BciT130I	CCWGG	2 cut(s)	236, 763
BciVI	GTATCC	2 cut(s)	167, 1156
BcnI	CCSGG	2 cut(s)	51, 170
BcoDI	GTCTC	4 cut(s)	170, 218, 1607, 1611
BcuI	ACTAGT	2 cut(s)	986, 2032
BfaI	CTAG	8 cut(s)	987, 1415, 1529, 1683, 1914, 1919, 2013, 2033
BfmI	CTRYAG	2 cut(s)	1113, 1518
BfoI	RGCGCY	1 cut(s)	141
BfuAI	ACCTGC	1 cut(s)	283
BfuI	GTATCC	2 cut(s)	167, 1156
BglII	AGATCT	1 cut(s)	261
BlpI	GCTNAGC	1 cut(s)	1821
Bme1390I	CCNGG	4 cut(s)	51, 170, 236, 763
Bme18I	GGWCC	1 cut(s)	999
BmeT110I	CYCGRG	1 cut(s)	1151
BmgT120I	GGNCC	4 cut(s)	31, 32, 999, 1149
BmiI	GGNNCC	7 cut(s)	33, 34, 199, 214, 570, 698, 783
BmrFI	CCNGG	4 cut(s)	51, 170, 236, 763
BmsI	GCATC	8 cut(s)	823, 1081, 1288, 1297, 1543, 1954, 1960, 1966
BpiI	GAAGAC	2 cut(s)	139, 1016
BpmI	CTGGAG	3 cut(s)	257, 410, 858
Bpu1102I	GCTNAGC	1 cut(s)	1821
BpuEI	CTTGAG	2 cut(s)	1287, 1814
BpuMI	CCSGG	2 cut(s)	51, 170
BsaHI	GRCGYC	2 cut(s)	144, 344
BsaI	GGTCTC	1 cut(s)	218
BsaXI	ACNNNNNCTCC	4 cut(s)	230, 260, 875, 905
Bsc4I	CCNNNNNNNGG	5 cut(s)	50, 110, 169, 491, 2164
Bse1I	ACTGG	1 cut(s)	785
Bse3DI	GCAATG	1 cut(s)	1425
BseBI	CCWGG	2 cut(s)	236, 763
BseGI	GGATG	3 cut(s)	45, 913, 1957
BseLI	CCNNNNNNNGG	5 cut(s)	50, 110, 169, 491, 2164
BseMI	GCAATG	1 cut(s)	1425
BseMII	CTCAG	6 cut(s)	148, 399, 745, 1602, 1812, 1964
BseNI	ACTGG	1 cut(s)	785
BsePI	GCGCGC	1 cut(s)	78
BseRI	GAGGAG	2 cut(s)	254, 531
BseSI	GKGCMC	1 cut(s)	35
Bsh1236I	CGCG	2 cut(s)	80, 2165
BshFI	GGCC	6 cut(s)	33, 54, 1150, 1379, 1666, 1935
BshNI	GGYRCC	2 cut(s)	197, 212
BsiHKCI	CYCGRG	1 cut(s)	1151
BsiSI	CCGG	3 cut(s)	51, 169, 347
BsiWI	CGTACG	1 cut(s)	87
BslFI	GGGAC	3 cut(s)	86, 490, 821
BslI	CCNNNNNNNGG	5 cut(s)	50, 110, 169, 491, 2164
BsmAI	GTCTC	4 cut(s)	170, 218, 1607, 1611
BsmBI	CGTCTC	2 cut(s)	170, 1607
BsmFI	GGGAC	3 cut(s)	86, 490, 821
BsmI	GAATGC	2 cut(s)	709, 1447
BsnI	GGCC	6 cut(s)	33, 54, 1150, 1379, 1666, 1935
Bso31I	GGTCTC	1 cut(s)	218
BsoBI	CYCGRG	1 cut(s)	1151
Bsp120I	GGGCCC	1 cut(s)	31
Bsp1286I	GDGCHC	1 cut(s)	35
Bsp143I	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
Bsp1720I	GCTNAGC	1 cut(s)	1821
Bsp19I	CCATGG	3 cut(s)	1380, 1667, 2215
BspACI	CCGC	6 cut(s)	216, 1181, 1778, 2110, 2163, 2165
BspANI	GGCC	6 cut(s)	33, 54, 1150, 1379, 1666, 1935
BspCNI	CTCAG	6 cut(s)	147, 400, 744, 1603, 1813, 1963
BspFNI	CGCG	2 cut(s)	80, 2165
BspLI	GGNNCC	7 cut(s)	33, 34, 199, 214, 570, 698, 783
BspMAI	CTGCAG	2 cut(s)	1117, 1522
BspMI	ACCTGC	1 cut(s)	283
BspPI	GGATC	4 cut(s)	563, 576, 774, 1313
BspT107I	GGYRCC	2 cut(s)	197, 212
BspTNI	GGTCTC	1 cut(s)	218
BsrDI	GCAATG	1 cut(s)	1425
BsrI	ACTGG	1 cut(s)	785
BssHII	GCGCGC	1 cut(s)	78
BssMI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
BssNI	GRCGYC	2 cut(s)	144, 344
BssSI	CACGAG	1 cut(s)	467
BssT1I	CCWWGG	6 cut(s)	24, 255, 490, 1380, 1667, 2215
Bst2BI	CACGAG	1 cut(s)	467
Bst2UI	CCWGG	2 cut(s)	236, 763
Bst4CI	ACNGT	7 cut(s)	184, 450, 536, 753, 958, 1205, 2020
Bst6I	CTCTTC	2 cut(s)	654, 1751
BstACI	GRCGYC	2 cut(s)	144, 344
BstAPI	GCANNNNNTGC	1 cut(s)	722
BstC8I	GCNNGC	5 cut(s)	80, 154, 1191, 1546, 2100
BstDEI	CTNAG	7 cut(s)	134, 408, 731, 1611, 1751, 1821, 1950
BstDSI	CCRYGG	6 cut(s)	219, 599, 1380, 1667, 2163, 2215
BstF5I	GGATG	3 cut(s)	45, 913, 1957
BstFNI	CGCG	2 cut(s)	80, 2165
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	4 cut(s)	80, 82, 140, 838
BstKTI	GATC	6 cut(s)	264, 523, 571, 769, 1321, 1489
BstMAI	GTCTC	4 cut(s)	170, 218, 1607, 1611
BstMBI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 713, 722, 1195, 1307, 1895, 2085
BstNI	CCWGG	2 cut(s)	236, 763
BstNSI	RCATGY	1 cut(s)	483
BstSCI	CCNGG	4 cut(s)	49, 168, 234, 761
BstSFI	CTRYAG	2 cut(s)	1113, 1518
BstSLI	GKGCMC	1 cut(s)	35
BstUI	CGCG	2 cut(s)	80, 2165
BstV2I	GAAGAC	2 cut(s)	139, 1016
BstX2I	RGATCY	3 cut(s)	261, 568, 766
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	3 cut(s)	261, 568, 766
BsuI	GTATCC	2 cut(s)	167, 1156
BsuRI	GGCC	6 cut(s)	33, 54, 1150, 1379, 1666, 1935
BtgI	CCRYGG	6 cut(s)	219, 599, 1380, 1667, 2163, 2215
BtgZI	GCGATG	1 cut(s)	2144
BtsCI	GGATG	3 cut(s)	45, 913, 1957
BtsI	GCAGTG	2 cut(s)	1527, 1815
BtsIMutI	CAGTG	6 cut(s)	530, 687, 1210, 1527, 1815, 2032
BveI	ACCTGC	1 cut(s)	283
Cac8I	GCNNGC	5 cut(s)	80, 154, 1191, 1546, 2100
CfoI	GCGC	4 cut(s)	80, 82, 140, 838
Cfr13I	GGNCC	4 cut(s)	31, 32, 999, 1149
Cfr42I	CCGCGG	1 cut(s)	2166
CseI	GACGC	3 cut(s)	352, 1624, 2068
Csp6I	GTAC	3 cut(s)	65, 88, 1679
CspCI	CAANNNNNGTGG	2 cut(s)	961, 996
CviAII	CATG	8 cut(s)	480, 794, 1176, 1381, 1564, 1668, 2107, 2216
CviQI	GTAC	3 cut(s)	65, 88, 1679
DdeI	CTNAG	7 cut(s)	134, 408, 731, 1611, 1751, 1821, 1950
DpnI	GATC	6 cut(s)	263, 522, 570, 768, 1320, 1488
DpnII	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
DraI	TTTAAA	1 cut(s)	549
DraIII	CACNNNGTG	2 cut(s)	958, 1631
EaeI	YGGCCR	2 cut(s)	52, 1664
Eam1104I	CTCTTC	2 cut(s)	654, 1751
EarI	CTCTTC	2 cut(s)	654, 1751
EciI	GGCGGA	2 cut(s)	1196, 1793
Eco130I	CCWWGG	6 cut(s)	24, 255, 490, 1380, 1667, 2215
Eco24I	GRGCYC	1 cut(s)	35
Eco31I	GGTCTC	1 cut(s)	218
Eco47I	GGWCC	1 cut(s)	999
Eco57I	CTGAAG	7 cut(s)	665, 787, 791, 1251, 1824, 1914, 2114
Eco88I	CYCGRG	1 cut(s)	1151
EcoO109I	RGGNCCY	2 cut(s)	31, 32
EcoRII	CCWGG	2 cut(s)	234, 761
EcoT14I	CCWWGG	6 cut(s)	24, 255, 490, 1380, 1667, 2215
EcoT22I	ATGCAT	1 cut(s)	1447
EcoT38I	GRGCYC	1 cut(s)	35
ErhI	CCWWGG	6 cut(s)	24, 255, 490, 1380, 1667, 2215
Esp3I	CGTCTC	2 cut(s)	170, 1607
FaeI	CATG	8 cut(s)	483, 797, 1179, 1384, 1567, 1671, 2110, 2219
FaqI	GGGAC	3 cut(s)	86, 490, 821
FatI	CATG	8 cut(s)	479, 793, 1175, 1380, 1563, 1667, 2106, 2215
FauI	CCCGC	1 cut(s)	2103
FblI	GTMKAC	1 cut(s)	593
FokI	GGATG	3 cut(s)	32, 920, 1944
FriOI	GRGCYC	1 cut(s)	35
FspBI	CTAG	8 cut(s)	987, 1415, 1529, 1683, 1914, 1919, 2013, 2033
GlaI	GCGC	4 cut(s)	79, 81, 139, 837
GsuI	CTGGAG	3 cut(s)	257, 410, 858
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	6 cut(s)	33, 54, 1150, 1379, 1666, 1935
HapII	CCGG	3 cut(s)	51, 169, 347
HgaI	GACGC	3 cut(s)	352, 1624, 2068
HhaI	GCGC	4 cut(s)	80, 82, 140, 838
Hin1I	GRCGYC	2 cut(s)	144, 344
Hin1II	CATG	8 cut(s)	483, 797, 1179, 1384, 1567, 1671, 2110, 2219
Hin6I	GCGC	4 cut(s)	78, 80, 138, 836
HinP1I	GCGC	4 cut(s)	78, 80, 138, 836
HincII	GTYRAC	2 cut(s)	94, 594
HindII	GTYRAC	2 cut(s)	94, 594
HindIII	AAGCTT	2 cut(s)	542, 1472
HinfI	GANTC	8 cut(s)	283, 595, 604, 898, 1283, 1407, 1745, 2065
HpaII	CCGG	3 cut(s)	51, 169, 347
HphI	GGTGA	3 cut(s)	64, 751, 970
Hpy166II	GTNNAC	3 cut(s)	94, 594, 2030
Hpy188I	TCNGA	8 cut(s)	409, 734, 771, 1162, 1318, 1849, 1953, 2070
Hpy8I	GTNNAC	3 cut(s)	94, 594, 2030
Hpy99I	CGWCG	2 cut(s)	146, 346
HpyAV	CCTTC	9 cut(s)	467, 640, 978, 1275, 1381, 1430, 1570, 1997, 2065
HpyCH4III	ACNGT	7 cut(s)	184, 450, 536, 753, 958, 1205, 2020
HpyCH4IV	ACGT	4 cut(s)	63, 90, 99, 144
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 713, 722, 1195, 1307, 1895, 2085
HpyF3I	CTNAG	7 cut(s)	134, 408, 731, 1611, 1751, 1821, 1950
HpySE526I	ACGT	4 cut(s)	63, 90, 99, 144
Hsp92I	GRCGYC	2 cut(s)	144, 344
Hsp92II	CATG	8 cut(s)	483, 797, 1179, 1384, 1567, 1671, 2110, 2219
HspAI	GCGC	4 cut(s)	78, 80, 138, 836
KspI	CCGCGG	1 cut(s)	2166
Kzo9I	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
LmnI	GCTCC	5 cut(s)	45, 349, 696, 1363, 1505
LweI	GCATC	8 cut(s)	823, 1081, 1288, 1297, 1543, 1954, 1960, 1966
MaeI	CTAG	8 cut(s)	987, 1415, 1529, 1683, 1914, 1919, 2013, 2033
MaeII	ACGT	4 cut(s)	63, 90, 99, 144
MaeIII	GTNAC	4 cut(s)	16, 1021, 1688, 2153
MalI	GATC	6 cut(s)	263, 522, 570, 768, 1320, 1488
MboI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
MflI	RGATCY	3 cut(s)	261, 568, 766
MhlI	GDGCHC	1 cut(s)	35
MlyI	GAGTC	3 cut(s)	589, 1277, 1401
MmeI	TCCRAC	3 cut(s)	636, 1054, 1140
Mph1103I	ATGCAT	1 cut(s)	1447
MroXI	GAANNNNTTC	1 cut(s)	1054
MseI	TTAA	9 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599, 2118
MslI	CAYNNNNRTG	3 cut(s)	959, 1508, 1962
MspA1I	CMGCKG	1 cut(s)	2165
MspI	CCGG	3 cut(s)	51, 169, 347
MspR9I	CCNGG	4 cut(s)	51, 170, 236, 763
Mva1269I	GAATGC	2 cut(s)	709, 1447
MvaI	CCWGG	2 cut(s)	236, 763
MvnI	CGCG	2 cut(s)	80, 2165
MwoI	GCNNNNNNNGC	7 cut(s)	135, 713, 722, 1195, 1307, 1895, 2085
NciI	CCSGG	2 cut(s)	51, 170
NcoI	CCATGG	3 cut(s)	1380, 1667, 2215
NdeII	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
NlaIII	CATG	8 cut(s)	483, 797, 1179, 1384, 1567, 1671, 2110, 2219
NlaIV	GGNNCC	7 cut(s)	33, 34, 199, 214, 570, 698, 783
NmeAIII	GCCGAG	1 cut(s)	90
NmuCI	GTSAC	2 cut(s)	1688, 2153
NsiI	ATGCAT	1 cut(s)	1447
NspI	RCATGY	1 cut(s)	483
PaqCI	CACCTGC	1 cut(s)	283
PauI	GCGCGC	1 cut(s)	78
PcsI	WCGNNNNNNNCGW	2 cut(s)	96, 160
PctI	GAATGC	2 cut(s)	709, 1447
PdmI	GAANNNNTTC	1 cut(s)	1054
PfeI	GAWTC	5 cut(s)	283, 604, 898, 1745, 2065
Pfl23II	CGTACG	1 cut(s)	87
PleI	GAGTC	3 cut(s)	589, 1277, 1401
PpsI	GAGTC	3 cut(s)	589, 1277, 1401
PshBI	ATTAAT	1 cut(s)	669
PsiI	TTATAA	1 cut(s)	2189
Psp6I	CCWGG	2 cut(s)	234, 761
PspGI	CCWGG	2 cut(s)	234, 761
PspLI	CGTACG	1 cut(s)	87
PspN4I	GGNNCC	7 cut(s)	33, 34, 199, 214, 570, 698, 783
PspOMI	GGGCCC	1 cut(s)	31
PspPI	GGNCC	4 cut(s)	31, 32, 999, 1149
PsrI	GAACNNNNNNTAC	2 cut(s)	290, 322
PstI	CTGCAG	2 cut(s)	1117, 1522
PsuI	RGATCY	3 cut(s)	261, 568, 766
PteI	GCGCGC	1 cut(s)	78
RsaI	GTAC	3 cut(s)	66, 89, 1680
RsaNI	GTAC	3 cut(s)	65, 88, 1679
RseI	CAYNNNNRTG	3 cut(s)	959, 1508, 1962
SacII	CCGCGG	1 cut(s)	2166
SalI	GTCGAC	1 cut(s)	592
SaqAI	TTAA	9 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599, 2118
Sau3AI	GATC	6 cut(s)	261, 520, 568, 766, 1318, 1486
Sau96I	GGNCC	4 cut(s)	31, 32, 999, 1149
SchI	GAGTC	3 cut(s)	589, 1277, 1401
ScrFI	CCNGG	4 cut(s)	51, 170, 236, 763
SduI	GDGCHC	1 cut(s)	35
SfaNI	GCATC	8 cut(s)	823, 1081, 1288, 1297, 1543, 1954, 1960, 1966
SfcI	CTRYAG	2 cut(s)	1113, 1518
Sfr303I	CCGCGG	1 cut(s)	2166
SgrBI	CCGCGG	1 cut(s)	2166
SinI	GGWCC	1 cut(s)	999
SmiMI	CAYNNNNRTG	3 cut(s)	959, 1508, 1962
SmlI	CTYRAG	2 cut(s)	1302, 1829
SmoI	CTYRAG	2 cut(s)	1302, 1829
SpeI	ACTAGT	2 cut(s)	986, 2032
SsiI	CCGC	6 cut(s)	216, 1181, 1778, 2110, 2163, 2165
SspI	AATATT	1 cut(s)	313
SspMI	CTAG	8 cut(s)	987, 1415, 1529, 1683, 1914, 1919, 2013, 2033
StyD4I	CCNGG	4 cut(s)	49, 168, 234, 761
StyI	CCWWGG	6 cut(s)	24, 255, 490, 1380, 1667, 2215
TaaI	ACNGT	7 cut(s)	184, 450, 536, 753, 958, 1205, 2020
TaiI	ACGT	4 cut(s)	66, 93, 102, 147
TaqI	TCGA	2 cut(s)	341, 593
TfiI	GAWTC	5 cut(s)	283, 604, 898, 1745, 2065
Tru1I	TTAA	9 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599, 2118
Tru9I	TTAA	9 cut(s)	548, 563, 669, 744, 1004, 1236, 1590, 1599, 2118
TscAI	CASTG	6 cut(s)	530, 687, 1210, 1527, 1815, 2032
TseFI	GTSAC	2 cut(s)	1688, 2153
Tsp45I	GTSAC	2 cut(s)	1688, 2153
TspDTI	ATGAA	6 cut(s)	275, 1022, 1056, 1222, 1552, 1752
TspGWI	ACGGA	3 cut(s)	236, 371, 616
TspRI	CASTG	6 cut(s)	530, 687, 1210, 1527, 1815, 2032
VpaK11BI	GGWCC	1 cut(s)	999
VspI	ATTAAT	1 cut(s)	669
XapI	RAATTY	1 cut(s)	326
XbaI	TCTAGA	1 cut(s)	1913
XceI	RCATGY	1 cut(s)	483
XmiI	GTMKAC	1 cut(s)	593
XmnI	GAANNNNTTC	1 cut(s)	1054
XspI	CTAG	8 cut(s)	987, 1415, 1529, 1683, 1914, 1919, 2013, 2033
ZraI	GACGTC	1 cut(s)	145
Zsp2I	ATGCAT	1 cut(s)	1447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.