Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Reverse (-)

77008501 .. 77012765

Gene Structure 4265 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G049370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2058 bp

ATGGGAGACCTCGCCAAGCAGGACCAGAGCGTCCCCAAGCTCCCCATCCCGGGGGAGCGAAACATCCTGATCACTAGTGCCTTGCCATATGTTAACAACGTCCCCCACCTCGGGAACATCATTGGCTGCGTCCTGAGCGCCGATGTCTTTGCTCGGTACTGCCGTCTCCGGGGATACAATACCGTCTATATCTCCGGGACCGATGAGTATGGCACAGCGACGGAGACCAAAGCCATGGAAGAGAAGTGCAGCCCCCAAGAGATATGTGACAAGTATCACAAAATCCATAGGGAGGTTTATGAGTGGTTCAACATAAGCTTCGACCAATTCGGCCGAACTTCCACGCCGCAGCAAACTGAGGTCTGCCAACAAATCTTCAAGAAATTGTTGGACAGTGATCGCCTTGCTGAAAACACAATCCAGCAGCTCCATTGTGACTCATGCAAAAGATTCTTGGCGGACCGATTCGTGGAGGGGACATGCCCTACTCAAGGATGTTACTATGATTCTGCTCGAGGAGACCAATGTGACAAGTGTGGAAAGCTTTTGAATCCCACGGAACTGAAAGATCCTAAATGCAAGGTATGCAAGGAAACTCCACGGATTCGCAAAACAAATCACTTGTTTCTGGAGCTCCCTTTGCTCAAGGATAAGTTGGAAGACTACATCAATAAGGCATCAGTGGAAGGGTGTTGGAGTCAGAATGCGGTTCAAATCACAAATGCCTGGCTCAAAGAGGGGCTTAAACAACGTTGCATTACAAGGGATCTCAAGTGGGGTGTTCCGGTTCCACACGATGGCTTCAGGGACAAGGTATTCTACGTGTGGTTCGATGCTCCTATTGGATACATCTCCATTACTAAGTGTTACACTCCTGATCACTGGGAGAAGTGGTGGAAGAATCCTGAGGATGTCGAATTGTATCAGTTTATGGGCAAGGACAATGTGCCATTTCACACGGTCATGTTTCCTTCCGCGCTTCTTGGAACAGGAGAAAATTGGACACTCATGAAGAACATTAGCGTCACTGAATACTTGAACTATGAAGAAGGCAAGTTTTCCAAGAGTAGAGGCATTGGAGTTTTTGGAAACGATGCAAAAGGAACGGGCATTCCAGTTGAAGTTTGGCGTTATTACCTGCTGACTAATAGGCCTGAGGTATCAGACACATTGTTCACCTGGGCCGATTTACAAGCAAAACTCAAAAGTGAGTTGGTGGGCAATTTGGGCAACTTCATCAACAGAGTTTTGAGTTTCGTTGCCAAACCTTCACTCAAACTAAAGCAAGGGCTGAAAATAGCCATGAGCATTTCTAGCGAGGGGAACGCATATCTCCAAGAATCCAAGTTCTGGAAGCTCTACAAGGAGGACCGACCTCGGTGCTCCATTGTCATTAAAACTTCAGTAGGGTTAGTGCATCTTCTTGCTTGCTTATTGGAACCTTACATGCCCTCATTTTCTGCTAGAGTACTTGAGCAGCTAAACTTGCCTCCTGAGTCGATCAAATCCCTTTGTGATGTCAATAAAGCAAAACGGCCTTGGGAGATCGTACCTGATGGTCACAAGATTGGAGAACCCAAGACATTGTTCACGACGTTGAACGGCGAAGAAGTCGAATCTCTGCGGAAAAAGTTTTCTGGAAGCCAAGCTGATAGGGAAAACGTACACAATAAGCTTTTACTAGCTCAAGAACACAGAGGGACTGACCGATTCACTAAATGGATGTCCTTCCAAAAGAATATACCCTGTCATATAATTGTTCAAACTGTGCTCAATATAGCTAGCAAAGCCATAAGCTATGCTGAAATGAGAACTGAACAATTGCATTATTCATTACCTTCTCTTTCAAAAGCCGCTCATTCTCCTCATCTAATTCAACCACCAATGACTCCAACTCAACTTGGTAAGCCTAAAATCAGAAAAATACACAACCAGTCCAGGCAAATATTCAAAATTCAAACTCACAAACAGCATGAACACCATAAGTTGACCAATCAAACATCATGTATGCACCAAGTTAAATTTAGCCTTCAGTTCAGTTCAGCCTTTCCAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g tRNA_bind

Protein Analysis

685

Amino Acids

78.45

Weight (kDa)

8.88

Isoelectric Point (pI)

40.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	22 - 417	1.4e-154	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	24 - 264	1.1e-08	tRNA synthetases class I (I, L, M and V)
Anticodon_3	PF19303	436 - 549	1.4e-15	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	29
Acc36I	ACCTGC	1 cut(s)	1146
AccB7I	CCANNNNNTGG	2 cut(s)	797, 1350
AccBSI	CCGCTC	1 cut(s)	1856
AccII	CGCG	1 cut(s)	977
AciI	CCGC	6 cut(s)	347, 458, 707, 975, 1624, 1854
AclI	AACGTT	1 cut(s)	751
AclWI	GGATC	2 cut(s)	563, 774
AcoI	YGGCCR	1 cut(s)	331
AcsI	RAATTY	2 cut(s)	1953, 2021
AcuI	CTGAAG	3 cut(s)	787, 1386, 2015
AfaI	GTAC	4 cut(s)	158, 1470, 1551, 1665
AflIII	ACRYGT	1 cut(s)	822
AhlI	ACTAGT	1 cut(s)	74
AjnI	CCWGG	3 cut(s)	725, 1178, 1937
AjuI	GAANNNNNNNTTGG	2 cut(s)	1571, 1603
AloI	GAACNNNNNNTCC	2 cut(s)	1096, 1128
Alw21I	GWGCWC	3 cut(s)	636, 1385, 1773
Alw26I	GTCTC	3 cut(s)	170, 218, 513
AlwI	GGATC	2 cut(s)	563, 774
Ama87I	CYCGRG	3 cut(s)	49, 110, 513
AoxI	GGCC	4 cut(s)	331, 1151, 1182, 1535
ApeKI	GCWGC	5 cut(s)	126, 249, 349, 424, 1477
ApoI	RAATTY	2 cut(s)	1953, 2021
AspLEI	GCGC	2 cut(s)	140, 979
AspS9I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1369
AsuC2I	CCSGG	4 cut(s)	50, 51, 170, 196
AsuHPI	GGTGA	1 cut(s)	1168
AsuNHI	GCTAGC	1 cut(s)	1781
AvaI	CYCGRG	3 cut(s)	49, 110, 513
AvaII	GGWCC	4 cut(s)	22, 198, 460, 1369
AxyI	CCTNAGG	2 cut(s)	906, 1155
BaeI	ACNNNNGTAYC	2 cut(s)	148, 181
BanII	GRGCYC	1 cut(s)	636
BbsI	GAAGAC	1 cut(s)	666
Bbv12I	GWGCWC	3 cut(s)	636, 1385, 1773
BbvI	GCAGC	5 cut(s)	113, 261, 361, 436, 1489
BccI	CCATC	3 cut(s)	53, 791, 1550
BceAI	ACGGC	3 cut(s)	147, 1550, 1618
BcgI	CGANNNNNNTGC	4 cut(s)	131, 165, 1362, 1396
BciT130I	CCWGG	3 cut(s)	727, 1180, 1939
BciVI	GTATCC	2 cut(s)	167, 839
BclI	TGATCA	2 cut(s)	69, 877
BcnI	CCSGG	4 cut(s)	50, 51, 170, 196
BcoDI	GTCTC	3 cut(s)	170, 218, 513
BcuI	ACTAGT	1 cut(s)	74
BfaI	CTAG	5 cut(s)	75, 1314, 1464, 1682, 1782
BfoI	RGCGCY	1 cut(s)	141
BfuAI	ACCTGC	1 cut(s)	1146
BfuI	GTATCC	2 cut(s)	167, 839
BisI	GCNGC	7 cut(s)	127, 250, 347, 350, 425, 1478, 1854
BlsI	GCNGC	7 cut(s)	128, 251, 348, 351, 426, 1479, 1855
BmcAI	AGTACT	1 cut(s)	1470
Bme1390I	CCNGG	7 cut(s)	50, 51, 170, 196, 727, 1180, 1939
Bme18I	GGWCC	4 cut(s)	22, 198, 460, 1369
BmeT110I	CYCGRG	3 cut(s)	49, 110, 513
BmgT120I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1369
BmiI	GGNNCC	3 cut(s)	199, 789, 1440
BmrFI	CCNGG	7 cut(s)	50, 51, 170, 196, 727, 1180, 1939
BmrI	ACTGGG	1 cut(s)	892
BmsI	GCATC	4 cut(s)	686, 823, 1084, 1426
BmtI	GCTAGC	1 cut(s)	1785
BmuI	ACTGGG	1 cut(s)	892
BpiI	GAAGAC	1 cut(s)	666
BpmI	CTGGAG	1 cut(s)	650
Bpu10I	CCTNAGC	1 cut(s)	134
BpuEI	CTTGAG	5 cut(s)	474, 629, 755, 1493, 1671
BpuMI	CCSGG	4 cut(s)	50, 51, 170, 196
BsaAI	YACGTR	1 cut(s)	823
BsaI	GGTCTC	2 cut(s)	218, 513
BsaWI	WCCGGW	1 cut(s)	784
BsaXI	ACNNNNNCTCC	2 cut(s)	878, 908
Bse1I	ACTGG	3 cut(s)	887, 1115, 1933
Bse21I	CCTNAGG	2 cut(s)	906, 1155
BseBI	CCWGG	3 cut(s)	727, 1180, 1939
BseGI	GGATG	5 cut(s)	45, 63, 500, 916, 1728
BseMII	CTCAG	5 cut(s)	125, 348, 897, 1146, 1485
BseNI	ACTGG	3 cut(s)	887, 1115, 1933
BseRI	GAGGAG	2 cut(s)	531, 1854
BseX3I	CGGCCG	1 cut(s)	331
BseXI	GCAGC	5 cut(s)	113, 261, 361, 436, 1489
BsgI	GTGCAG	1 cut(s)	268
Bsh1236I	CGCG	1 cut(s)	977
Bsh1285I	CGRYCG	1 cut(s)	334
BshFI	GGCC	4 cut(s)	333, 1153, 1184, 1537
BsiEI	CGRYCG	1 cut(s)	334
BsiHKAI	GWGCWC	3 cut(s)	636, 1385, 1773
BsiHKCI	CYCGRG	3 cut(s)	49, 110, 513
BsiSI	CCGG	4 cut(s)	50, 169, 195, 785
BslFI	GGGAC	6 cut(s)	17, 86, 211, 490, 821, 1714
BsmAI	GTCTC	3 cut(s)	170, 218, 513
BsmBI	CGTCTC	1 cut(s)	170
BsmFI	GGGAC	6 cut(s)	17, 86, 211, 490, 821, 1714
BsmI	GAATGC	2 cut(s)	709, 1110
BsnI	GGCC	4 cut(s)	333, 1153, 1184, 1537
Bso31I	GGTCTC	2 cut(s)	218, 513
BsoBI	CYCGRG	3 cut(s)	49, 110, 513
Bsp1286I	GDGCHC	3 cut(s)	636, 1385, 1773
Bsp143I	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
Bsp19I	CCATGG	1 cut(s)	234
BspACI	CCGC	6 cut(s)	347, 458, 707, 975, 1624, 1854
BspANI	GGCC	4 cut(s)	333, 1153, 1184, 1537
BspCNI	CTCAG	5 cut(s)	126, 349, 898, 1147, 1486
BspFNI	CGCG	1 cut(s)	977
BspHI	TCATGA	1 cut(s)	1008
BspLI	GGNNCC	3 cut(s)	199, 789, 1440
BspMI	ACCTGC	1 cut(s)	1146
BspOI	GCTAGC	1 cut(s)	1785
BspPI	GGATC	2 cut(s)	563, 774
BspTNI	GGTCTC	2 cut(s)	218, 513
BsrBI	CCGCTC	1 cut(s)	1856
BsrI	ACTGG	3 cut(s)	887, 1115, 1933
BssMI	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
BssT1I	CCWWGG	2 cut(s)	234, 1538
Bst2UI	CCWGG	3 cut(s)	727, 1180, 1939
Bst4CI	ACNGT	4 cut(s)	184, 395, 961, 1768
Bst6I	CTCTTC	1 cut(s)	234
BstAPI	GCANNNNNTGC	1 cut(s)	585
BstBAI	YACGTR	1 cut(s)	823
BstC8I	GCNNGC	2 cut(s)	1429, 1783
BstDEI	CTNAG	6 cut(s)	134, 357, 861, 906, 1155, 1494
BstDSI	CCRYGG	3 cut(s)	234, 555, 599
BstENI	CCTNNNNNAGG	1 cut(s)	489
BstF5I	GGATG	5 cut(s)	45, 63, 500, 916, 1728
BstFNI	CGCG	1 cut(s)	977
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	2 cut(s)	140, 979
BstKTI	GATC	7 cut(s)	72, 400, 571, 769, 880, 1503, 1548
BstMAI	GTCTC	3 cut(s)	170, 218, 513
BstMBI	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
BstMCI	CGRYCG	1 cut(s)	334
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 585, 640, 1227, 1314, 1486, 1787
BstNI	CCWGG	3 cut(s)	727, 1180, 1939
BstNSI	RCATGY	2 cut(s)	483, 1450
BstSCI	CCNGG	7 cut(s)	48, 49, 168, 194, 725, 1178, 1937
BstUI	CGCG	1 cut(s)	977
BstV1I	GCAGC	5 cut(s)	113, 261, 361, 436, 1489
BstV2I	GAAGAC	1 cut(s)	666
BstX2I	RGATCY	2 cut(s)	568, 766
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	2 cut(s)	568, 766
BstZI	CGGCCG	1 cut(s)	331
Bsu36I	CCTNAGG	2 cut(s)	906, 1155
BsuI	GTATCC	2 cut(s)	167, 839
BsuRI	GGCC	4 cut(s)	333, 1153, 1184, 1537
BtgI	CCRYGG	3 cut(s)	234, 555, 599
BtsCI	GGATG	5 cut(s)	45, 63, 500, 916, 1728
BtsIMutI	CAGTG	4 cut(s)	400, 687, 880, 1026
BveI	ACCTGC	1 cut(s)	1146
Cac8I	GCNNGC	2 cut(s)	1429, 1783
CciI	TCATGA	1 cut(s)	1008
CfoI	GCGC	2 cut(s)	140, 979
Cfr13I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1369
Cfr9I	CCCGGG	1 cut(s)	49
CpoI	CGGWCCG	1 cut(s)	460
CseI	GACGC	3 cut(s)	19, 118, 1012
Csp6I	GTAC	4 cut(s)	157, 1469, 1550, 1664
CspI	CGGWCCG	1 cut(s)	460
CviAII	CATG	9 cut(s)	235, 441, 480, 964, 1009, 1303, 1447, 1973, 2004
CviQI	GTAC	4 cut(s)	157, 1469, 1550, 1664
DdeI	CTNAG	6 cut(s)	134, 357, 861, 906, 1155, 1494
DpnI	GATC	7 cut(s)	71, 399, 570, 768, 879, 1502, 1547
DpnII	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
DrdI	GACNNNNNNGTC	1 cut(s)	29
DseDI	GACNNNNNNGTC	1 cut(s)	29
EaeI	YGGCCR	1 cut(s)	331
EagI	CGGCCG	1 cut(s)	331
Eam1104I	CTCTTC	1 cut(s)	234
EarI	CTCTTC	1 cut(s)	234
EciI	GGCGGA	1 cut(s)	473
Ecl136II	GAGCTC	1 cut(s)	634
EclXI	CGGCCG	1 cut(s)	331
Eco130I	CCWWGG	2 cut(s)	234, 1538
Eco147I	AGGCCT	1 cut(s)	1153
Eco24I	GRGCYC	1 cut(s)	636
Eco31I	GGTCTC	2 cut(s)	218, 513
Eco47I	GGWCC	4 cut(s)	22, 198, 460, 1369
Eco52I	CGGCCG	1 cut(s)	331
Eco53kI	GAGCTC	1 cut(s)	634
Eco57I	CTGAAG	3 cut(s)	787, 1386, 2015
Eco81I	CCTNAGG	2 cut(s)	906, 1155
Eco88I	CYCGRG	3 cut(s)	49, 110, 513
EcoICRI	GAGCTC	1 cut(s)	634
EcoNI	CCTNNNNNAGG	1 cut(s)	489
EcoRII	CCWGG	3 cut(s)	725, 1178, 1937
EcoT14I	CCWWGG	2 cut(s)	234, 1538
EcoT38I	GRGCYC	1 cut(s)	636
ErhI	CCWWGG	2 cut(s)	234, 1538
Esp3I	CGTCTC	1 cut(s)	170
FaeI	CATG	9 cut(s)	238, 444, 483, 967, 1012, 1306, 1450, 1976, 2007
FalI	AAGNNNNNCTT	2 cut(s)	726, 758
FaqI	GGGAC	6 cut(s)	17, 86, 211, 490, 821, 1714
FatI	CATG	9 cut(s)	234, 440, 479, 963, 1008, 1302, 1446, 1972, 2003
FauNDI	CATATG	1 cut(s)	88
FbaI	TGATCA	2 cut(s)	69, 877
Fnu4HI	GCNGC	7 cut(s)	127, 250, 347, 350, 425, 1478, 1854
FokI	GGATG	5 cut(s)	32, 50, 507, 923, 1735
FriOI	GRGCYC	1 cut(s)	636
Fsp4HI	GCNGC	7 cut(s)	127, 250, 347, 350, 425, 1478, 1854
FspBI	CTAG	5 cut(s)	75, 1314, 1464, 1682, 1782
GlaI	GCGC	2 cut(s)	139, 978
GluI	GCNGC	7 cut(s)	127, 250, 347, 350, 425, 1478, 1854
GsuI	CTGGAG	1 cut(s)	650
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	4 cut(s)	333, 1153, 1184, 1537
HapII	CCGG	4 cut(s)	50, 169, 195, 785
HgaI	GACGC	3 cut(s)	19, 118, 1012
HhaI	GCGC	2 cut(s)	140, 979
Hin1II	CATG	9 cut(s)	238, 444, 483, 967, 1012, 1306, 1450, 1976, 2007
Hin6I	GCGC	2 cut(s)	138, 977
HinP1I	GCGC	2 cut(s)	138, 977
HincII	GTYRAC	2 cut(s)	94, 1989
HindII	GTYRAC	2 cut(s)	94, 1989
HindIII	AAGCTT	3 cut(s)	316, 542, 1673
HpaI	GTTAAC	1 cut(s)	94
HpaII	CCGG	4 cut(s)	50, 169, 195, 785
HphI	GGTGA	1 cut(s)	1168
Hpy166II	GTNNAC	5 cut(s)	94, 1176, 1590, 1666, 1989
Hpy188I	TCNGA	3 cut(s)	702, 1165, 1919
Hpy8I	GTNNAC	5 cut(s)	94, 1176, 1590, 1666, 1989
Hpy99I	CGWCG	2 cut(s)	223, 1597
HpyAV	CCTTC	7 cut(s)	680, 981, 1043, 1278, 1738, 1848, 2039
HpyCH4III	ACNGT	4 cut(s)	184, 395, 961, 1768
HpyCH4IV	ACGT	5 cut(s)	99, 751, 822, 1595, 1662
HpyCH4V	TGCA	9 cut(s)	249, 444, 579, 588, 756, 1097, 1417, 1825, 2011
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 585, 640, 1227, 1314, 1486, 1787
HpyF3I	CTNAG	6 cut(s)	134, 357, 861, 906, 1155, 1494
HpySE526I	ACGT	5 cut(s)	99, 751, 822, 1595, 1662
Hsp92II	CATG	9 cut(s)	238, 444, 483, 967, 1012, 1306, 1450, 1976, 2007
HspAI	GCGC	2 cut(s)	138, 977
Ksp22I	TGATCA	2 cut(s)	69, 877
KspAI	GTTAAC	1 cut(s)	94
Kzo9I	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
LmnI	GCTCC	7 cut(s)	45, 55, 432, 631, 639, 841, 1388
Lsp1109I	GCAGC	5 cut(s)	113, 261, 361, 436, 1489
LweI	GCATC	4 cut(s)	686, 823, 1084, 1426
MaeI	CTAG	5 cut(s)	75, 1314, 1464, 1682, 1782
MaeII	ACGT	5 cut(s)	99, 751, 822, 1595, 1662
MaeIII	GTNAC	7 cut(s)	266, 434, 497, 527, 866, 1024, 1559
MalI	GATC	7 cut(s)	71, 399, 570, 768, 879, 1502, 1547
MbiI	CCGCTC	1 cut(s)	1856
MboI	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
MboII	GAAGA	8 cut(s)	251, 367, 671, 910, 1024, 1058, 1412, 1619
MfeI	CAATTG	1 cut(s)	1820
MflI	RGATCY	2 cut(s)	568, 766
MhlI	GDGCHC	3 cut(s)	636, 1385, 1773
MlyI	GAGTC	4 cut(s)	431, 706, 1505, 1882
MmeI	TCCRAC	4 cut(s)	369, 636, 674, 1916
MseI	TTAA	5 cut(s)	93, 744, 1395, 2019, 2056
MslI	CAYNNNNRTG	1 cut(s)	962
MspI	CCGG	4 cut(s)	50, 169, 195, 785
MspR9I	CCNGG	7 cut(s)	50, 51, 170, 196, 727, 1180, 1939
MunI	CAATTG	1 cut(s)	1820
Mva1269I	GAATGC	2 cut(s)	709, 1110
MvaI	CCWGG	3 cut(s)	727, 1180, 1939
MvnI	CGCG	1 cut(s)	977
MwoI	GCNNNNNNNGC	7 cut(s)	135, 585, 640, 1227, 1314, 1486, 1787
NciI	CCSGG	4 cut(s)	50, 51, 170, 196
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	88
NdeII	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
NheI	GCTAGC	1 cut(s)	1781
NlaIII	CATG	9 cut(s)	238, 444, 483, 967, 1012, 1306, 1450, 1976, 2007
NlaIV	GGNNCC	3 cut(s)	199, 789, 1440
NmuCI	GTSAC	5 cut(s)	266, 434, 527, 1024, 1559
NspI	RCATGY	2 cut(s)	483, 1450
PaeR7I	CTCGAG	1 cut(s)	513
PagI	TCATGA	1 cut(s)	1008
PceI	AGGCCT	1 cut(s)	1153
PcsI	WCGNNNNNNNCGW	2 cut(s)	160, 828
PctI	GAATGC	2 cut(s)	709, 1110
PfeI	GAWTC	9 cut(s)	450, 465, 506, 550, 604, 901, 1340, 1616, 1710
PflMI	CCANNNNNTGG	2 cut(s)	797, 1350
PfoI	TCCNGGA	1 cut(s)	194
PkrI	GCNGC	7 cut(s)	128, 251, 348, 351, 426, 1479, 1855
PleI	GAGTC	4 cut(s)	431, 705, 1504, 1882
PpsI	GAGTC	4 cut(s)	431, 705, 1504, 1882
Ppu21I	YACGTR	1 cut(s)	823
Psp124BI	GAGCTC	1 cut(s)	636
Psp1406I	AACGTT	1 cut(s)	751
Psp6I	CCWGG	3 cut(s)	725, 1178, 1937
PspGI	CCWGG	3 cut(s)	725, 1178, 1937
PspN4I	GGNNCC	3 cut(s)	199, 789, 1440
PspPI	GGNCC	5 cut(s)	22, 198, 460, 1182, 1369
PspXI	VCTCGAGB	1 cut(s)	513
PsuI	RGATCY	2 cut(s)	568, 766
RsaI	GTAC	4 cut(s)	158, 1470, 1551, 1665
RsaNI	GTAC	4 cut(s)	157, 1469, 1550, 1664
RseI	CAYNNNNRTG	1 cut(s)	962
Rsr2I	CGGWCCG	1 cut(s)	460
RsrII	CGGWCCG	1 cut(s)	460
SacI	GAGCTC	1 cut(s)	636
SaqAI	TTAA	5 cut(s)	93, 744, 1395, 2019, 2056
SatI	GCNGC	7 cut(s)	127, 250, 347, 350, 425, 1478, 1854
Sau3AI	GATC	7 cut(s)	69, 397, 568, 766, 877, 1500, 1545
Sau96I	GGNCC	5 cut(s)	22, 198, 460, 1182, 1369
ScaI	AGTACT	1 cut(s)	1470
SchI	GAGTC	4 cut(s)	431, 706, 1505, 1882
ScrFI	CCNGG	7 cut(s)	50, 51, 170, 196, 727, 1180, 1939
SduI	GDGCHC	3 cut(s)	636, 1385, 1773
SfaNI	GCATC	4 cut(s)	686, 823, 1084, 1426
Sfr274I	CTCGAG	1 cut(s)	513
SinI	GGWCC	4 cut(s)	22, 198, 460, 1369
SlaI	CTCGAG	1 cut(s)	513
SmaI	CCCGGG	1 cut(s)	51
SmiMI	CAYNNNNRTG	1 cut(s)	962
SmlI	CTYRAG	6 cut(s)	489, 513, 644, 770, 1472, 1686
SmoI	CTYRAG	6 cut(s)	489, 513, 644, 770, 1472, 1686
SpeI	ACTAGT	1 cut(s)	74
SseBI	AGGCCT	1 cut(s)	1153
SsiI	CCGC	6 cut(s)	347, 458, 707, 975, 1624, 1854
SspI	AATATT	1 cut(s)	1947
SspMI	CTAG	5 cut(s)	75, 1314, 1464, 1682, 1782
SstI	GAGCTC	1 cut(s)	636
StuI	AGGCCT	1 cut(s)	1153
StyD4I	CCNGG	7 cut(s)	48, 49, 168, 194, 725, 1178, 1937
StyI	CCWWGG	2 cut(s)	234, 1538
TaaI	ACNGT	4 cut(s)	184, 395, 961, 1768
TaiI	ACGT	5 cut(s)	102, 754, 825, 1598, 1665
TaqI	TCGA	6 cut(s)	321, 514, 831, 915, 1499, 1614
TaqII	GACCGA	4 cut(s)	215, 477, 1386, 1722
TatI	WGTACW	1 cut(s)	1468
TauI	GCSGC	2 cut(s)	349, 1856
TfiI	GAWTC	9 cut(s)	450, 465, 506, 550, 604, 901, 1340, 1616, 1710
Tru1I	TTAA	5 cut(s)	93, 744, 1395, 2019, 2056
Tru9I	TTAA	5 cut(s)	93, 744, 1395, 2019, 2056
TscAI	CASTG	4 cut(s)	400, 687, 887, 1033
TseFI	GTSAC	5 cut(s)	266, 434, 527, 1024, 1559
TseI	GCWGC	5 cut(s)	126, 249, 349, 424, 1477
Tsp45I	GTSAC	5 cut(s)	266, 434, 527, 1024, 1559
TspDTI	ATGAA	5 cut(s)	1025, 1059, 1225, 1821, 1989
TspGWI	ACGGA	3 cut(s)	236, 572, 616
TspMI	CCCGGG	1 cut(s)	49
TspRI	CASTG	4 cut(s)	400, 687, 887, 1033
Van91I	CCANNNNNTGG	2 cut(s)	797, 1350
VpaK11BI	GGWCC	4 cut(s)	22, 198, 460, 1369
XagI	CCTNNNNNAGG	1 cut(s)	489
XapI	RAATTY	2 cut(s)	1953, 2021
XceI	RCATGY	2 cut(s)	483, 1450
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1122
XhoI	CTCGAG	1 cut(s)	513
XmaI	CCCGGG	1 cut(s)	49
XspI	CTAG	5 cut(s)	75, 1314, 1464, 1682, 1782
ZrmI	AGTACT	1 cut(s)	1470

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.