Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 3 cut(s) | 470, 1058, 1685 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 409, 848, 1090 |
| AccI | GTMKAC | 3 cut(s) | 351, 501, 1215 |
| AccII | CGCG | 1 cut(s) | 116 |
| AciI | CCGC | 4 cut(s) | 26, 111, 114, 1065 |
| AclWI | GGATC | 1 cut(s) | 165 |
| AcoI | YGGCCR | 4 cut(s) | 69, 387, 483, 1038 |
| AcsI | RAATTY | 3 cut(s) | 700, 717, 1408 |
| AcyI | GRCGYC | 1 cut(s) | 300 |
| AfaI | GTAC | 4 cut(s) | 784, 1265, 1436, 1584 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 229, 409, 848, 1090 |
| AgsI | TTSAA | 6 cut(s) | 662, 696, 1000, 1057, 1367, 1396 |
| AhlI | ACTAGT | 1 cut(s) | 1571 |
| AjnI | CCWGG | 3 cut(s) | 402, 1558, 1591 |
| Alw21I | GWGCWC | 2 cut(s) | 381, 1641 |
| Alw26I | GTCTC | 1 cut(s) | 1607 |
| AlwI | GGATC | 1 cut(s) | 165 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 334, 409, 430, 1094 |
| Ama87I | CYCGRG | 1 cut(s) | 722 |
| AoxI | GGCC | 9 cut(s) | 69, 306, 363, 387, 483, 1038, 1421, 1468, 1589 |
| ApeKI | GCWGC | 9 cut(s) | 331, 394, 492, 547, 809, 928, 1235, 1238, 1370 |
| ApoI | RAATTY | 3 cut(s) | 700, 717, 1408 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1208, 1240 |
| AseI | ATTAAT | 1 cut(s) | 945 |
| AspS9I | GGNCC | 4 cut(s) | 421, 1070, 1469, 1556 |
| AsuC2I | CCSGG | 1 cut(s) | 650 |
| AsuHPI | GGTGA | 3 cut(s) | 84, 722, 1550 |
| AsuII | TTCGAA | 1 cut(s) | 1140 |
| AvaI | CYCGRG | 1 cut(s) | 722 |
| AvaII | GGWCC | 3 cut(s) | 421, 1070, 1556 |
| BaeGI | GKGCMC | 1 cut(s) | 882 |
| BalI | TGGCCA | 2 cut(s) | 485, 1040 |
| BanII | GRGCYC | 1 cut(s) | 381 |
| BauI | CACGAG | 1 cut(s) | 511 |
| BbsI | GAAGAC | 1 cut(s) | 1237 |
| Bbv12I | GWGCWC | 2 cut(s) | 381, 1641 |
| BbvI | GCAGC | 9 cut(s) | 343, 381, 504, 559, 796, 915, 1222, 1225, 1357 |
| BccI | CCATC | 8 cut(s) | 97, 173, 218, 374, 464, 842, 932, 977 |
| BceAI | ACGGC | 2 cut(s) | 132, 637 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 481, 515 |
| BciT130I | CCWGG | 3 cut(s) | 404, 1560, 1593 |
| BclI | TGATCA | 2 cut(s) | 1449, 1476 |
| BcnI | CCSGG | 1 cut(s) | 650 |
| BcoDI | GTCTC | 1 cut(s) | 1607 |
| BcuI | ACTAGT | 1 cut(s) | 1571 |
| BfaI | CTAG | 3 cut(s) | 507, 1440, 1572 |
| BfmI | CTRYAG | 3 cut(s) | 553, 1212, 1692 |
| BfuAI | ACCTGC | 3 cut(s) | 470, 1058, 1685 |
| Bme1390I | CCNGG | 4 cut(s) | 404, 650, 1560, 1593 |
| Bme18I | GGWCC | 3 cut(s) | 421, 1070, 1556 |
| BmeT110I | CYCGRG | 1 cut(s) | 722 |
| BmgT120I | GGNCC | 4 cut(s) | 421, 1070, 1469, 1556 |
| BmiI | GGNNCC | 1 cut(s) | 654 |
| BmrFI | CCNGG | 4 cut(s) | 404, 650, 1560, 1593 |
| BmsI | GCATC | 1 cut(s) | 1121 |
| BpiI | GAAGAC | 1 cut(s) | 1237 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 353, 385 |
| BpmI | CTGGAG | 1 cut(s) | 1581 |
| Bpu14I | TTCGAA | 1 cut(s) | 1140 |
| BpuEI | CTTGAG | 2 cut(s) | 365, 584 |
| BpuMI | CCSGG | 1 cut(s) | 650 |
| BsaHI | GRCGYC | 1 cut(s) | 300 |
| BsaJI | CCNNGG | 2 cut(s) | 303, 1424 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 227, 257, 266, 296, 368, 398 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 229, 409, 848, 1090 |
| Bse1I | ACTGG | 1 cut(s) | 967 |
| Bse3DI | GCAATG | 3 cut(s) | 224, 1237, 1392 |
| BseBI | CCWGG | 3 cut(s) | 404, 1560, 1593 |
| BseDI | CCNNGG | 2 cut(s) | 303, 1424 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 229, 409, 848, 1090 |
| BseMI | GCAATG | 3 cut(s) | 224, 1237, 1392 |
| BseMII | CTCAG | 3 cut(s) | 69, 425, 647 |
| BseNI | ACTGG | 1 cut(s) | 967 |
| BseRI | GAGGAG | 5 cut(s) | 26, 88, 91, 274, 277 |
| BseSI | GKGCMC | 1 cut(s) | 882 |
| BseX3I | CGGCCG | 2 cut(s) | 69, 387 |
| BseXI | GCAGC | 9 cut(s) | 343, 381, 504, 559, 796, 915, 1222, 1225, 1357 |
| BsgI | GTGCAG | 4 cut(s) | 807, 1290, 1342, 1356 |
| Bsh1236I | CGCG | 1 cut(s) | 116 |
| Bsh1285I | CGRYCG | 2 cut(s) | 72, 390 |
| BshFI | GGCC | 9 cut(s) | 71, 308, 365, 389, 485, 1040, 1423, 1470, 1591 |
| BsiEI | CGRYCG | 2 cut(s) | 72, 390 |
| BsiHKAI | GWGCWC | 2 cut(s) | 381, 1641 |
| BsiHKCI | CYCGRG | 1 cut(s) | 722 |
| BsiSI | CCGG | 1 cut(s) | 649 |
| BslFI | GGGAC | 5 cut(s) | 501, 887, 1326, 1522, 1670 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 229, 409, 848, 1090 |
| BsmAI | GTCTC | 1 cut(s) | 1607 |
| BsmFI | GGGAC | 5 cut(s) | 501, 887, 1326, 1522, 1670 |
| BsmI | GAATGC | 2 cut(s) | 1298, 1615 |
| BsnI | GGCC | 9 cut(s) | 71, 308, 365, 389, 485, 1040, 1423, 1470, 1591 |
| BsoBI | CYCGRG | 1 cut(s) | 722 |
| Bsp119I | TTCGAA | 1 cut(s) | 1140 |
| Bsp1286I | GDGCHC | 3 cut(s) | 381, 882, 1641 |
| Bsp143I | GATC | 3 cut(s) | 170, 1449, 1476 |
| BspACI | CCGC | 4 cut(s) | 26, 111, 114, 1065 |
| BspANI | GGCC | 9 cut(s) | 71, 308, 365, 389, 485, 1040, 1423, 1470, 1591 |
| BspCNI | CTCAG | 3 cut(s) | 70, 426, 646 |
| BspFNI | CGCG | 1 cut(s) | 116 |
| BspHI | TCATGA | 1 cut(s) | 1605 |
| BspLI | GGNNCC | 1 cut(s) | 654 |
| BspMAI | CTGCAG | 1 cut(s) | 1696 |
| BspMI | ACCTGC | 3 cut(s) | 470, 1058, 1685 |
| BspPI | GGATC | 1 cut(s) | 165 |
| BspQI | GCTCTTC | 1 cut(s) | 911 |
| BspT104I | TTCGAA | 1 cut(s) | 1140 |
| BsrDI | GCAATG | 3 cut(s) | 224, 1237, 1392 |
| BsrI | ACTGG | 1 cut(s) | 967 |
| BssECI | CCNNGG | 2 cut(s) | 303, 1424 |
| BssMI | GATC | 3 cut(s) | 170, 1449, 1476 |
| BssNI | GRCGYC | 1 cut(s) | 300 |
| BssSI | CACGAG | 1 cut(s) | 511 |
| BssT1I | CCWWGG | 1 cut(s) | 1424 |
| Bst2BI | CACGAG | 1 cut(s) | 511 |
| Bst2UI | CCWGG | 3 cut(s) | 404, 1560, 1593 |
| Bst4CI | ACNGT | 7 cut(s) | 557, 763, 1213, 1447, 1513, 1601, 1636 |
| Bst6I | CTCTTC | 3 cut(s) | 57, 680, 911 |
| BstACI | GRCGYC | 1 cut(s) | 300 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1304 |
| BstBI | TTCGAA | 1 cut(s) | 1140 |
| BstC8I | GCNNGC | 2 cut(s) | 118, 329 |
| BstDEI | CTNAG | 5 cut(s) | 78, 434, 633, 680, 1175 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 227 |
| BstFNI | CGCG | 1 cut(s) | 116 |
| BstKTI | GATC | 3 cut(s) | 173, 1452, 1479 |
| BstMAI | GTCTC | 1 cut(s) | 1607 |
| BstMBI | GATC | 3 cut(s) | 170, 1449, 1476 |
| BstMCI | CGRYCG | 2 cut(s) | 72, 390 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 113, 340, 1304, 1619 |
| BstNI | CCWGG | 3 cut(s) | 404, 1560, 1593 |
| BstSCI | CCNGG | 4 cut(s) | 402, 648, 1558, 1591 |
| BstSFI | CTRYAG | 3 cut(s) | 553, 1212, 1692 |
| BstSLI | GKGCMC | 1 cut(s) | 882 |
| BstUI | CGCG | 1 cut(s) | 116 |
| BstV1I | GCAGC | 9 cut(s) | 343, 381, 504, 559, 796, 915, 1222, 1225, 1357 |
| BstV2I | GAAGAC | 1 cut(s) | 1237 |
| BstX2I | RGATCY | 1 cut(s) | 170 |
| BstYI | RGATCY | 1 cut(s) | 170 |
| BstZI | CGGCCG | 2 cut(s) | 69, 387 |
| BsuRI | GGCC | 9 cut(s) | 71, 308, 365, 389, 485, 1040, 1423, 1470, 1591 |
| BtsI | GCAGTG | 3 cut(s) | 745, 1095, 1302 |
| BtsIMutI | CAGTG | 7 cut(s) | 562, 745, 759, 1095, 1302, 1486, 1641 |
| BveI | ACCTGC | 3 cut(s) | 470, 1058, 1685 |
| Cac8I | GCNNGC | 2 cut(s) | 118, 329 |
| CaiI | CAGNNNCTG | 4 cut(s) | 334, 409, 430, 1094 |
| CciI | TCATGA | 1 cut(s) | 1605 |
| Cfr13I | GGNCC | 4 cut(s) | 421, 1070, 1469, 1556 |
| CseI | GACGC | 1 cut(s) | 308 |
| Csp6I | GTAC | 4 cut(s) | 783, 1264, 1435, 1583 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 38 |
| CviAII | CATG | 6 cut(s) | 292, 779, 1279, 1453, 1553, 1606 |
| CviQI | GTAC | 4 cut(s) | 783, 1264, 1435, 1583 |
| DdeI | CTNAG | 5 cut(s) | 78, 434, 633, 680, 1175 |
| DpnI | GATC | 3 cut(s) | 172, 1451, 1478 |
| DpnII | GATC | 3 cut(s) | 170, 1449, 1476 |
| EaeI | YGGCCR | 4 cut(s) | 69, 387, 483, 1038 |
| EagI | CGGCCG | 2 cut(s) | 69, 387 |
| Eam1104I | CTCTTC | 3 cut(s) | 57, 680, 911 |
| EarI | CTCTTC | 3 cut(s) | 57, 680, 911 |
| EciI | GGCGGA | 1 cut(s) | 100 |
| Ecl136II | GAGCTC | 1 cut(s) | 379 |
| EclXI | CGGCCG | 2 cut(s) | 69, 387 |
| Eco130I | CCWWGG | 1 cut(s) | 1424 |
| Eco147I | AGGCCT | 1 cut(s) | 1423 |
| Eco24I | GRGCYC | 1 cut(s) | 381 |
| Eco47I | GGWCC | 3 cut(s) | 421, 1070, 1556 |
| Eco52I | CGGCCG | 2 cut(s) | 69, 387 |
| Eco53kI | GAGCTC | 1 cut(s) | 379 |
| Eco88I | CYCGRG | 1 cut(s) | 722 |
| EcoICRI | GAGCTC | 1 cut(s) | 379 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 227 |
| EcoRI | GAATTC | 1 cut(s) | 717 |
| EcoRII | CCWGG | 3 cut(s) | 402, 1558, 1591 |
| EcoT14I | CCWWGG | 1 cut(s) | 1424 |
| EcoT38I | GRGCYC | 1 cut(s) | 381 |
| ErhI | CCWWGG | 1 cut(s) | 1424 |
| FaeI | CATG | 6 cut(s) | 295, 782, 1282, 1456, 1556, 1609 |
| FaqI | GGGAC | 5 cut(s) | 501, 887, 1326, 1522, 1670 |
| FatI | CATG | 6 cut(s) | 291, 778, 1278, 1452, 1552, 1605 |
| FbaI | TGATCA | 2 cut(s) | 1449, 1476 |
| FblI | GTMKAC | 3 cut(s) | 351, 501, 1215 |
| FriOI | GRGCYC | 1 cut(s) | 381 |
| FspBI | CTAG | 3 cut(s) | 507, 1440, 1572 |
| GsuI | CTGGAG | 1 cut(s) | 1581 |
| HaeIII | GGCC | 9 cut(s) | 71, 308, 365, 389, 485, 1040, 1423, 1470, 1591 |
| HapII | CCGG | 1 cut(s) | 649 |
| HgaI | GACGC | 1 cut(s) | 308 |
| Hin1I | GRCGYC | 1 cut(s) | 300 |
| Hin1II | CATG | 6 cut(s) | 295, 782, 1282, 1456, 1556, 1609 |
| HincII | GTYRAC | 2 cut(s) | 352, 502 |
| HindII | GTYRAC | 2 cut(s) | 352, 502 |
| HinfI | GANTC | 9 cut(s) | 80, 154, 427, 514, 772, 818, 1327, 1341, 1549 |
| HpaII | CCGG | 1 cut(s) | 649 |
| HphI | GGTGA | 3 cut(s) | 84, 722, 1550 |
| Hpy166II | GTNNAC | 7 cut(s) | 126, 352, 415, 502, 776, 1216, 1435 |
| Hpy188I | TCNGA | 5 cut(s) | 170, 184, 340, 935, 1476 |
| Hpy8I | GTNNAC | 7 cut(s) | 126, 352, 415, 502, 776, 1216, 1435 |
| Hpy99I | CGWCG | 3 cut(s) | 302, 353, 376 |
| HpyAV | CCTTC | 6 cut(s) | 541, 1063, 1136, 1413, 1423, 1696 |
| HpyCH4III | ACNGT | 7 cut(s) | 557, 763, 1213, 1447, 1513, 1601, 1636 |
| HpyCH4IV | ACGT | 1 cut(s) | 348 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 113, 340, 1304, 1619 |
| HpyF3I | CTNAG | 5 cut(s) | 78, 434, 633, 680, 1175 |
| HpySE526I | ACGT | 1 cut(s) | 348 |
| Hsp92I | GRCGYC | 1 cut(s) | 300 |
| Hsp92II | CATG | 6 cut(s) | 295, 782, 1282, 1456, 1556, 1609 |
| Ksp22I | TGATCA | 2 cut(s) | 1449, 1476 |
| Kzo9I | GATC | 3 cut(s) | 170, 1449, 1476 |
| LguI | GCTCTTC | 1 cut(s) | 911 |
| LmnI | GCTCC | 7 cut(s) | 287, 340, 376, 406, 652, 806, 1562 |
| Lsp1109I | GCAGC | 9 cut(s) | 343, 381, 504, 559, 796, 915, 1222, 1225, 1357 |
| LweI | GCATC | 1 cut(s) | 1121 |
| MaeI | CTAG | 3 cut(s) | 507, 1440, 1572 |
| MaeII | ACGT | 1 cut(s) | 348 |
| MaeIII | GTNAC | 6 cut(s) | 445, 574, 638, 1048, 1196, 1595 |
| MalI | GATC | 3 cut(s) | 172, 1451, 1478 |
| MboI | GATC | 3 cut(s) | 170, 1449, 1476 |
| MboII | GAAGA | 7 cut(s) | 44, 684, 697, 857, 928, 1216, 1237 |
| MflI | RGATCY | 1 cut(s) | 170 |
| MhlI | GDGCHC | 3 cut(s) | 381, 882, 1641 |
| MlsI | TGGCCA | 2 cut(s) | 485, 1040 |
| MluNI | TGGCCA | 2 cut(s) | 485, 1040 |
| MlyI | GAGTC | 5 cut(s) | 89, 421, 523, 781, 827 |
| MmeI | TCCRAC | 4 cut(s) | 207, 318, 1476, 1495 |
| Mox20I | TGGCCA | 2 cut(s) | 485, 1040 |
| MscI | TGGCCA | 2 cut(s) | 485, 1040 |
| MseI | TTAA | 4 cut(s) | 753, 945, 1700, 1711 |
| Msp20I | TGGCCA | 2 cut(s) | 485, 1040 |
| MspA1I | CMGCKG | 2 cut(s) | 28, 334 |
| MspI | CCGG | 1 cut(s) | 649 |
| MspR9I | CCNGG | 4 cut(s) | 404, 650, 1560, 1593 |
| Mva1269I | GAATGC | 2 cut(s) | 1298, 1615 |
| MvaI | CCWGG | 3 cut(s) | 404, 1560, 1593 |
| MvnI | CGCG | 1 cut(s) | 116 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 113, 340, 1304, 1619 |
| NciI | CCSGG | 1 cut(s) | 650 |
| NdeII | GATC | 3 cut(s) | 170, 1449, 1476 |
| NlaIII | CATG | 6 cut(s) | 295, 782, 1282, 1456, 1556, 1609 |
| NlaIV | GGNNCC | 1 cut(s) | 654 |
| NmeAIII | GCCGAG | 4 cut(s) | 30, 47, 328, 415 |
| NmuCI | GTSAC | 3 cut(s) | 445, 638, 1196 |
| NspV | TTCGAA | 1 cut(s) | 1140 |
| PaeR7I | CTCGAG | 1 cut(s) | 722 |
| PagI | TCATGA | 1 cut(s) | 1605 |
| PceI | AGGCCT | 1 cut(s) | 1423 |
| PciSI | GCTCTTC | 1 cut(s) | 911 |
| PctI | GAATGC | 2 cut(s) | 1298, 1615 |
| PfeI | GAWTC | 4 cut(s) | 154, 1327, 1341, 1549 |
| PflFI | GACNNNGTC | 2 cut(s) | 419, 761 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 409, 848, 1090 |
| PfoI | TCCNGGA | 1 cut(s) | 648 |
| PleI | GAGTC | 5 cut(s) | 88, 421, 522, 780, 826 |
| PpsI | GAGTC | 5 cut(s) | 88, 421, 522, 780, 826 |
| PshBI | ATTAAT | 1 cut(s) | 945 |
| Psp124BI | GAGCTC | 1 cut(s) | 381 |
| Psp6I | CCWGG | 3 cut(s) | 402, 1558, 1591 |
| PspGI | CCWGG | 3 cut(s) | 402, 1558, 1591 |
| PspN4I | GGNNCC | 1 cut(s) | 654 |
| PspPI | GGNCC | 4 cut(s) | 421, 1070, 1469, 1556 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 338, 370 |
| PstI | CTGCAG | 1 cut(s) | 1696 |
| PstNI | CAGNNNCTG | 4 cut(s) | 334, 409, 430, 1094 |
| PsuI | RGATCY | 1 cut(s) | 170 |
| PsyI | GACNNNGTC | 2 cut(s) | 419, 761 |
| PvuII | CAGCTG | 1 cut(s) | 334 |
| RsaI | GTAC | 4 cut(s) | 784, 1265, 1436, 1584 |
| RsaNI | GTAC | 4 cut(s) | 783, 1264, 1435, 1583 |
| SacI | GAGCTC | 1 cut(s) | 381 |
| SalI | GTCGAC | 2 cut(s) | 350, 500 |
| SapI | GCTCTTC | 1 cut(s) | 911 |
| SaqAI | TTAA | 4 cut(s) | 753, 945, 1700, 1711 |
| Sau3AI | GATC | 3 cut(s) | 170, 1449, 1476 |
| Sau96I | GGNCC | 4 cut(s) | 421, 1070, 1469, 1556 |
| SchI | GAGTC | 5 cut(s) | 89, 421, 523, 781, 827 |
| ScrFI | CCNGG | 4 cut(s) | 404, 650, 1560, 1593 |
| SduI | GDGCHC | 3 cut(s) | 381, 882, 1641 |
| SfaNI | GCATC | 1 cut(s) | 1121 |
| SfcI | CTRYAG | 3 cut(s) | 553, 1212, 1692 |
| Sfr274I | CTCGAG | 1 cut(s) | 722 |
| SfuI | TTCGAA | 1 cut(s) | 1140 |
| SinI | GGWCC | 3 cut(s) | 421, 1070, 1556 |
| SlaI | CTCGAG | 1 cut(s) | 722 |
| SmlI | CTYRAG | 3 cut(s) | 380, 599, 722 |
| SmoI | CTYRAG | 3 cut(s) | 380, 599, 722 |
| SpeI | ACTAGT | 1 cut(s) | 1571 |
| SseBI | AGGCCT | 1 cut(s) | 1423 |
| SsiI | CCGC | 4 cut(s) | 26, 111, 114, 1065 |
| SspMI | CTAG | 3 cut(s) | 507, 1440, 1572 |
| SstI | GAGCTC | 1 cut(s) | 381 |
| StuI | AGGCCT | 1 cut(s) | 1423 |
| StyD4I | CCNGG | 4 cut(s) | 402, 648, 1558, 1591 |
| StyI | CCWWGG | 1 cut(s) | 1424 |
| TaaI | ACNGT | 7 cut(s) | 557, 763, 1213, 1447, 1513, 1601, 1636 |
| TaiI | ACGT | 1 cut(s) | 351 |
| TaqII | GACCGA | 1 cut(s) | 311 |
| TatI | WGTACW | 2 cut(s) | 1434, 1582 |
| TauI | GCSGC | 2 cut(s) | 28, 116 |
| TfiI | GAWTC | 4 cut(s) | 154, 1327, 1341, 1549 |
| Tru1I | TTAA | 4 cut(s) | 753, 945, 1700, 1711 |
| Tru9I | TTAA | 4 cut(s) | 753, 945, 1700, 1711 |
| TscAI | CASTG | 7 cut(s) | 562, 745, 766, 1095, 1309, 1486, 1641 |
| TseFI | GTSAC | 3 cut(s) | 445, 638, 1196 |
| TseI | GCWGC | 9 cut(s) | 331, 394, 492, 547, 809, 928, 1235, 1238, 1370 |
| Tsp45I | GTSAC | 3 cut(s) | 445, 638, 1196 |
| TspDTI | ATGAA | 4 cut(s) | 49, 658, 1210, 1622 |
| TspGWI | ACGGA | 4 cut(s) | 213, 389, 800, 1221 |
| TspRI | CASTG | 7 cut(s) | 562, 745, 766, 1095, 1309, 1486, 1641 |
| Tth111I | GACNNNGTC | 2 cut(s) | 419, 761 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 409, 848, 1090 |
| VpaK11BI | GGWCC | 3 cut(s) | 421, 1070, 1556 |
| VspI | ATTAAT | 1 cut(s) | 945 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 227 |
| XapI | RAATTY | 3 cut(s) | 700, 717, 1408 |
| XhoI | CTCGAG | 1 cut(s) | 722 |
| XmiI | GTMKAC | 3 cut(s) | 351, 501, 1215 |
| XspI | CTAG | 3 cut(s) | 507, 1440, 1572 |