Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 7 cut(s) | 229, 261, 1844, 2176, 2249, 2402, 2448 |
| AclWI | GGATC | 4 cut(s) | 629, 840, 1379, 2240 |
| AcoI | YGGCCR | 1 cut(s) | 282 |
| AcsI | RAATTY | 2 cut(s) | 392, 669 |
| AcuI | CTGAAG | 5 cut(s) | 1317, 1569, 1890, 1980, 2180 |
| AcyI | GRCGYC | 1 cut(s) | 18 |
| AfaI | GTAC | 5 cut(s) | 43, 254, 731, 1097, 1746 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 116, 176, 235, 354, 557, 2230 |
| AhlI | ACTAGT | 1 cut(s) | 1594 |
| AjnI | CCWGG | 4 cut(s) | 115, 758, 827, 2287 |
| Alw26I | GTCTC | 5 cut(s) | 57, 284, 922, 1673, 2336 |
| AlwI | GGATC | 4 cut(s) | 629, 840, 1379, 2240 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 752 |
| AoxI | GGCC | 8 cut(s) | 96, 282, 1214, 1730, 1941, 1999, 2095, 2382 |
| ApeKI | GCWGC | 7 cut(s) | 487, 490, 1660, 1886, 1955, 2207, 2258 |
| ApoI | RAATTY | 2 cut(s) | 392, 669 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1974, 2006 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1120 |
| AspLEI | GCGC | 3 cut(s) | 146, 402, 1724 |
| AspS9I | GGNCC | 4 cut(s) | 88, 97, 756, 1552 |
| AsuHPI | GGTGA | 4 cut(s) | 130, 1067, 2078, 2312 |
| AsuII | TTCGAA | 1 cut(s) | 2213 |
| AsuNHI | GCTAGC | 1 cut(s) | 1718 |
| AvaII | GGWCC | 3 cut(s) | 88, 756, 1552 |
| AxyI | CCTNAGG | 1 cut(s) | 1218 |
| BauI | CACGAG | 2 cut(s) | 533, 579 |
| BbsI | GAAGAC | 1 cut(s) | 1082 |
| BbvI | GCAGC | 7 cut(s) | 499, 502, 1672, 1873, 1967, 2194, 2245 |
| BccI | CCATC | 2 cut(s) | 119, 1642 |
| BceAI | ACGGC | 3 cut(s) | 297, 1745, 1762 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 169, 197, 203, 231 |
| BciT130I | CCWGG | 4 cut(s) | 117, 760, 829, 2289 |
| BciVI | GTATCC | 1 cut(s) | 2443 |
| BcoDI | GTCTC | 5 cut(s) | 57, 284, 922, 1673, 2336 |
| BcuI | ACTAGT | 1 cut(s) | 1594 |
| BfaI | CTAG | 7 cut(s) | 1403, 1497, 1595, 1719, 1980, 2079, 2378 |
| BfmI | CTRYAG | 1 cut(s) | 1953 |
| BfoI | RGCGCY | 1 cut(s) | 147 |
| BfuI | GTATCC | 1 cut(s) | 2443 |
| BglII | AGATCT | 2 cut(s) | 327, 2356 |
| BisI | GCNGC | 8 cut(s) | 229, 488, 491, 1661, 1887, 1956, 2208, 2259 |
| BlsI | GCNGC | 8 cut(s) | 230, 489, 492, 1662, 1888, 1957, 2209, 2260 |
| BmcAI | AGTACT | 1 cut(s) | 1097 |
| Bme1390I | CCNGG | 4 cut(s) | 117, 760, 829, 2289 |
| Bme18I | GGWCC | 3 cut(s) | 88, 756, 1552 |
| BmgT120I | GGNCC | 4 cut(s) | 88, 97, 756, 1552 |
| BmiI | GGNNCC | 7 cut(s) | 99, 757, 764, 849, 855, 1351, 1623 |
| BmrFI | CCNGG | 4 cut(s) | 117, 760, 829, 2289 |
| BmsI | GCATC | 5 cut(s) | 889, 1354, 1609, 2020, 2032 |
| BmtI | GCTAGC | 1 cut(s) | 1722 |
| BpiI | GAAGAC | 1 cut(s) | 1082 |
| BpmI | CTGGAG | 1 cut(s) | 781 |
| Bpu14I | TTCGAA | 1 cut(s) | 2213 |
| BpuEI | CTTGAG | 5 cut(s) | 540, 695, 1837, 1880, 2247 |
| BsaAI | YACGTR | 1 cut(s) | 1242 |
| BsaHI | GRCGYC | 1 cut(s) | 18 |
| BsaI | GGTCTC | 3 cut(s) | 57, 284, 2336 |
| BsaJI | CCNNGG | 6 cut(s) | 115, 175, 828, 1733, 2229, 2288 |
| BsaWI | WCCGGW | 2 cut(s) | 850, 2133 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 941, 971 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 116, 176, 235, 354, 557, 2230 |
| Bse1I | ACTGG | 2 cut(s) | 1057, 1528 |
| Bse21I | CCTNAGG | 1 cut(s) | 1218 |
| Bse3DI | GCAATG | 3 cut(s) | 959, 1491, 2202 |
| BseBI | CCWGG | 4 cut(s) | 117, 760, 829, 2289 |
| BseDI | CCNNGG | 6 cut(s) | 115, 175, 828, 1733, 2229, 2288 |
| BseGI | GGATG | 3 cut(s) | 111, 129, 2023 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 116, 176, 235, 354, 557, 2230 |
| BseMI | GCAATG | 3 cut(s) | 959, 1491, 2202 |
| BseMII | CTCAG | 4 cut(s) | 960, 1209, 1668, 2030 |
| BseNI | ACTGG | 2 cut(s) | 1057, 1528 |
| BseRI | GAGGAG | 2 cut(s) | 597, 740 |
| BseXI | GCAGC | 7 cut(s) | 499, 502, 1672, 1873, 1967, 2194, 2245 |
| BseYI | CCCAGC | 1 cut(s) | 752 |
| BsgI | GTGCAG | 4 cut(s) | 561, 1395, 1872, 2345 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2088 |
| BshFI | GGCC | 8 cut(s) | 98, 284, 1216, 1732, 1943, 2001, 2097, 2384 |
| BsiEI | CGRYCG | 1 cut(s) | 2088 |
| BsiSI | CCGG | 2 cut(s) | 851, 2134 |
| BslFI | GGGAC | 3 cut(s) | 152, 292, 556 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 116, 176, 235, 354, 557, 2230 |
| BsmAI | GTCTC | 5 cut(s) | 57, 284, 922, 1673, 2336 |
| BsmBI | CGTCTC | 1 cut(s) | 1673 |
| BsmFI | GGGAC | 3 cut(s) | 152, 292, 556 |
| BsmI | GAATGC | 1 cut(s) | 775 |
| BsnI | GGCC | 8 cut(s) | 98, 284, 1216, 1732, 1943, 2001, 2097, 2384 |
| Bso31I | GGTCTC | 3 cut(s) | 57, 284, 2336 |
| Bsp119I | TTCGAA | 1 cut(s) | 2213 |
| Bsp1407I | TGTACA | 1 cut(s) | 252 |
| Bsp143I | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| BspACI | CCGC | 7 cut(s) | 229, 261, 1844, 2176, 2249, 2402, 2448 |
| BspANI | GGCC | 8 cut(s) | 98, 284, 1216, 1732, 1943, 2001, 2097, 2384 |
| BspCNI | CTCAG | 4 cut(s) | 961, 1210, 1669, 2029 |
| BspLI | GGNNCC | 7 cut(s) | 99, 757, 764, 849, 855, 1351, 1623 |
| BspMAI | CTGCAG | 1 cut(s) | 1957 |
| BspOI | GCTAGC | 1 cut(s) | 1722 |
| BspPI | GGATC | 4 cut(s) | 629, 840, 1379, 2240 |
| BspT104I | TTCGAA | 1 cut(s) | 2213 |
| BspTNI | GGTCTC | 3 cut(s) | 57, 284, 2336 |
| BsrDI | GCAATG | 3 cut(s) | 959, 1491, 2202 |
| BsrGI | TGTACA | 1 cut(s) | 252 |
| BsrI | ACTGG | 2 cut(s) | 1057, 1528 |
| BssECI | CCNNGG | 6 cut(s) | 115, 175, 828, 1733, 2229, 2288 |
| BssMI | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| BssNI | GRCGYC | 1 cut(s) | 18 |
| BssSI | CACGAG | 2 cut(s) | 533, 579 |
| BssT1I | CCWWGG | 1 cut(s) | 1733 |
| Bst2BI | CACGAG | 2 cut(s) | 533, 579 |
| Bst2UI | CCWGG | 4 cut(s) | 117, 760, 829, 2289 |
| Bst6I | CTCTTC | 2 cut(s) | 300, 2046 |
| BstACI | GRCGYC | 1 cut(s) | 18 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 788 |
| BstAUI | TGTACA | 1 cut(s) | 252 |
| BstBAI | YACGTR | 1 cut(s) | 1242 |
| BstBI | TTCGAA | 1 cut(s) | 2213 |
| BstC8I | GCNNGC | 4 cut(s) | 122, 1257, 1720, 2166 |
| BstDEI | CTNAG | 6 cut(s) | 520, 969, 1125, 1218, 1677, 2016 |
| BstDSI | CCRYGG | 1 cut(s) | 2229 |
| BstF5I | GGATG | 3 cut(s) | 111, 129, 2023 |
| BstH2I | RGCGCY | 1 cut(s) | 147 |
| BstHHI | GCGC | 3 cut(s) | 146, 402, 1724 |
| BstKTI | GATC | 9 cut(s) | 6, 330, 589, 637, 835, 1387, 1764, 2248, 2359 |
| BstMAI | GTCTC | 5 cut(s) | 57, 284, 922, 1673, 2336 |
| BstMBI | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| BstMCI | CGRYCG | 1 cut(s) | 2088 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 779, 788, 1373, 1669, 1690, 1961 |
| BstNI | CCWGG | 4 cut(s) | 117, 760, 829, 2289 |
| BstNSI | RCATGY | 2 cut(s) | 510, 549 |
| BstSCI | CCNGG | 4 cut(s) | 115, 758, 827, 2287 |
| BstSFI | CTRYAG | 1 cut(s) | 1953 |
| BstV1I | GCAGC | 7 cut(s) | 499, 502, 1672, 1873, 1967, 2194, 2245 |
| BstV2I | GAAGAC | 1 cut(s) | 1082 |
| BstX2I | RGATCY | 5 cut(s) | 327, 634, 832, 1384, 2356 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 301 |
| BstYI | RGATCY | 5 cut(s) | 327, 634, 832, 1384, 2356 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1218 |
| BsuI | GTATCC | 1 cut(s) | 2443 |
| BsuRI | GGCC | 8 cut(s) | 98, 284, 1216, 1732, 1943, 2001, 2097, 2384 |
| BtgI | CCRYGG | 1 cut(s) | 2229 |
| BtsCI | GGATG | 3 cut(s) | 111, 129, 2023 |
| BtsIMutI | CAGTG | 7 cut(s) | 37, 753, 936, 1927, 2224, 2370, 2427 |
| Cac8I | GCNNGC | 4 cut(s) | 122, 1257, 1720, 2166 |
| CaiI | CAGNNNCTG | 1 cut(s) | 752 |
| CfoI | GCGC | 3 cut(s) | 146, 402, 1724 |
| Cfr13I | GGNCC | 4 cut(s) | 88, 97, 756, 1552 |
| CseI | GACGC | 2 cut(s) | 26, 1690 |
| Csp6I | GTAC | 5 cut(s) | 42, 253, 730, 1096, 1745 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1027, 1062 |
| CviAII | CATG | 8 cut(s) | 58, 344, 507, 546, 792, 860, 1027, 2173 |
| CviQI | GTAC | 5 cut(s) | 42, 253, 730, 1096, 1745 |
| DdeI | CTNAG | 6 cut(s) | 520, 969, 1125, 1218, 1677, 2016 |
| DpnI | GATC | 9 cut(s) | 5, 329, 588, 636, 834, 1386, 1763, 2247, 2358 |
| DpnII | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| EaeI | YGGCCR | 1 cut(s) | 282 |
| Eam1104I | CTCTTC | 2 cut(s) | 300, 2046 |
| EarI | CTCTTC | 2 cut(s) | 300, 2046 |
| EciI | GGCGGA | 1 cut(s) | 2238 |
| Eco130I | CCWWGG | 1 cut(s) | 1733 |
| Eco147I | AGGCCT | 1 cut(s) | 1216 |
| Eco31I | GGTCTC | 3 cut(s) | 57, 284, 2336 |
| Eco47I | GGWCC | 3 cut(s) | 88, 756, 1552 |
| Eco57I | CTGAAG | 5 cut(s) | 1317, 1569, 1890, 1980, 2180 |
| Eco81I | CCTNAGG | 1 cut(s) | 1218 |
| EcoO109I | RGGNCCY | 2 cut(s) | 97, 756 |
| EcoRI | GAATTC | 1 cut(s) | 669 |
| EcoRII | CCWGG | 4 cut(s) | 115, 758, 827, 2287 |
| EcoT14I | CCWWGG | 1 cut(s) | 1733 |
| EcoT22I | ATGCAT | 1 cut(s) | 793 |
| ErhI | CCWWGG | 1 cut(s) | 1733 |
| Esp3I | CGTCTC | 1 cut(s) | 1673 |
| FaeI | CATG | 8 cut(s) | 61, 347, 510, 549, 795, 863, 1030, 2176 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 792, 824, 1717, 1749 |
| FaqI | GGGAC | 3 cut(s) | 152, 292, 556 |
| FatI | CATG | 8 cut(s) | 57, 343, 506, 545, 791, 859, 1026, 2172 |
| FauI | CCCGC | 2 cut(s) | 2169, 2441 |
| FauNDI | CATATG | 1 cut(s) | 257 |
| Fnu4HI | GCNGC | 8 cut(s) | 229, 488, 491, 1661, 1887, 1956, 2208, 2259 |
| FokI | GGATG | 3 cut(s) | 98, 116, 2010 |
| Fsp4HI | GCNGC | 8 cut(s) | 229, 488, 491, 1661, 1887, 1956, 2208, 2259 |
| FspBI | CTAG | 7 cut(s) | 1403, 1497, 1595, 1719, 1980, 2079, 2378 |
| GlaI | GCGC | 3 cut(s) | 145, 401, 1723 |
| GluI | GCNGC | 8 cut(s) | 229, 488, 491, 1661, 1887, 1956, 2208, 2259 |
| GsaI | CCCAGC | 1 cut(s) | 756 |
| GsuI | CTGGAG | 1 cut(s) | 781 |
| HaeII | RGCGCY | 1 cut(s) | 147 |
| HaeIII | GGCC | 8 cut(s) | 98, 284, 1216, 1732, 1943, 2001, 2097, 2384 |
| HapII | CCGG | 2 cut(s) | 851, 2134 |
| HgaI | GACGC | 2 cut(s) | 26, 1690 |
| HhaI | GCGC | 3 cut(s) | 146, 402, 1724 |
| Hin1I | GRCGYC | 1 cut(s) | 18 |
| Hin1II | CATG | 8 cut(s) | 61, 347, 510, 549, 795, 863, 1030, 2176 |
| Hin6I | GCGC | 3 cut(s) | 144, 400, 1722 |
| HinP1I | GCGC | 3 cut(s) | 144, 400, 1722 |
| HindIII | AAGCTT | 3 cut(s) | 382, 608, 1538 |
| HinfI | GANTC | 7 cut(s) | 616, 964, 1379, 1442, 1473, 1811, 2036 |
| HpaII | CCGG | 2 cut(s) | 851, 2134 |
| HphI | GGTGA | 4 cut(s) | 130, 1067, 2078, 2312 |
| Hpy166II | GTNNAC | 2 cut(s) | 253, 1038 |
| Hpy188I | TCNGA | 8 cut(s) | 475, 621, 1228, 1384, 1592, 1915, 2019, 2160 |
| Hpy8I | GTNNAC | 2 cut(s) | 253, 1038 |
| Hpy99I | CGWCG | 3 cut(s) | 14, 17, 20 |
| HpyCH4IV | ACGT | 3 cut(s) | 165, 220, 1241 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 779, 788, 1373, 1669, 1690, 1961 |
| HpyF3I | CTNAG | 6 cut(s) | 520, 969, 1125, 1218, 1677, 2016 |
| HpySE526I | ACGT | 3 cut(s) | 165, 220, 1241 |
| Hsp92I | GRCGYC | 1 cut(s) | 18 |
| Hsp92II | CATG | 8 cut(s) | 61, 347, 510, 549, 795, 863, 1030, 2176 |
| HspAI | GCGC | 3 cut(s) | 144, 400, 1722 |
| Kzo9I | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| LmnI | GCTCC | 6 cut(s) | 762, 907, 949, 1355, 1372, 1429 |
| Lsp1109I | GCAGC | 7 cut(s) | 499, 502, 1672, 1873, 1967, 2194, 2245 |
| LweI | GCATC | 5 cut(s) | 889, 1354, 1609, 2020, 2032 |
| MaeI | CTAG | 7 cut(s) | 1403, 1497, 1595, 1719, 1980, 2079, 2378 |
| MaeII | ACGT | 3 cut(s) | 165, 220, 1241 |
| MalI | GATC | 9 cut(s) | 5, 329, 588, 636, 834, 1386, 1763, 2247, 2358 |
| MboI | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| MflI | RGATCY | 5 cut(s) | 327, 634, 832, 1384, 2356 |
| MlyI | GAGTC | 1 cut(s) | 1467 |
| MmeI | TCCRAC | 4 cut(s) | 435, 644, 1570, 1599 |
| Mph1103I | ATGCAT | 1 cut(s) | 793 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1120 |
| MseI | TTAA | 6 cut(s) | 375, 810, 1100, 1458, 1656, 2184 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2028 |
| MspA1I | CMGCKG | 2 cut(s) | 1958, 2417 |
| MspI | CCGG | 2 cut(s) | 851, 2134 |
| MspR9I | CCNGG | 4 cut(s) | 117, 760, 829, 2289 |
| Mva1269I | GAATGC | 1 cut(s) | 775 |
| MvaI | CCWGG | 4 cut(s) | 117, 760, 829, 2289 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 779, 788, 1373, 1669, 1690, 1961 |
| NdeI | CATATG | 1 cut(s) | 257 |
| NdeII | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| NheI | GCTAGC | 1 cut(s) | 1718 |
| NlaIII | CATG | 8 cut(s) | 61, 347, 510, 549, 795, 863, 1030, 2176 |
| NlaIV | GGNNCC | 7 cut(s) | 99, 757, 764, 849, 855, 1351, 1623 |
| NmeAIII | GCCGAG | 1 cut(s) | 156 |
| NmuCI | GTSAC | 6 cut(s) | 35, 332, 500, 563, 1754, 2219 |
| NsiI | ATGCAT | 1 cut(s) | 793 |
| NspI | RCATGY | 2 cut(s) | 510, 549 |
| NspV | TTCGAA | 1 cut(s) | 2213 |
| PceI | AGGCCT | 1 cut(s) | 1216 |
| PctI | GAATGC | 1 cut(s) | 775 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1120 |
| PfeI | GAWTC | 6 cut(s) | 616, 964, 1379, 1442, 1811, 2036 |
| PfoI | TCCNGGA | 1 cut(s) | 758 |
| PkrI | GCNGC | 8 cut(s) | 230, 489, 492, 1662, 1888, 1957, 2209, 2260 |
| PleI | GAGTC | 1 cut(s) | 1467 |
| PpsI | GAGTC | 1 cut(s) | 1467 |
| Ppu21I | YACGTR | 1 cut(s) | 1242 |
| PpuMI | RGGWCCY | 1 cut(s) | 756 |
| Psp5II | RGGWCCY | 1 cut(s) | 756 |
| Psp6I | CCWGG | 4 cut(s) | 115, 758, 827, 2287 |
| PspFI | CCCAGC | 1 cut(s) | 752 |
| PspGI | CCWGG | 4 cut(s) | 115, 758, 827, 2287 |
| PspN4I | GGNNCC | 7 cut(s) | 99, 757, 764, 849, 855, 1351, 1623 |
| PspPI | GGNCC | 4 cut(s) | 88, 97, 756, 1552 |
| PspPPI | RGGWCCY | 1 cut(s) | 756 |
| PstI | CTGCAG | 1 cut(s) | 1957 |
| PstNI | CAGNNNCTG | 1 cut(s) | 752 |
| PsuI | RGATCY | 5 cut(s) | 327, 634, 832, 1384, 2356 |
| PvuII | CAGCTG | 2 cut(s) | 1958, 2417 |
| RsaI | GTAC | 5 cut(s) | 43, 254, 731, 1097, 1746 |
| RsaNI | GTAC | 5 cut(s) | 42, 253, 730, 1096, 1745 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2028 |
| SaqAI | TTAA | 6 cut(s) | 375, 810, 1100, 1458, 1656, 2184 |
| SatI | GCNGC | 8 cut(s) | 229, 488, 491, 1661, 1887, 1956, 2208, 2259 |
| Sau3AI | GATC | 9 cut(s) | 3, 327, 586, 634, 832, 1384, 1761, 2245, 2356 |
| Sau96I | GGNCC | 4 cut(s) | 88, 97, 756, 1552 |
| ScaI | AGTACT | 1 cut(s) | 1097 |
| SchI | GAGTC | 1 cut(s) | 1467 |
| ScrFI | CCNGG | 4 cut(s) | 117, 760, 829, 2289 |
| SfaNI | GCATC | 5 cut(s) | 889, 1354, 1609, 2020, 2032 |
| SfcI | CTRYAG | 1 cut(s) | 1953 |
| SfuI | TTCGAA | 1 cut(s) | 2213 |
| SinI | GGWCC | 3 cut(s) | 88, 756, 1552 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2028 |
| SmlI | CTYRAG | 5 cut(s) | 555, 710, 1816, 1895, 2262 |
| SmoI | CTYRAG | 5 cut(s) | 555, 710, 1816, 1895, 2262 |
| SpeI | ACTAGT | 1 cut(s) | 1594 |
| SseBI | AGGCCT | 1 cut(s) | 1216 |
| SsiI | CCGC | 7 cut(s) | 229, 261, 1844, 2176, 2249, 2402, 2448 |
| SspMI | CTAG | 7 cut(s) | 1403, 1497, 1595, 1719, 1980, 2079, 2378 |
| StuI | AGGCCT | 1 cut(s) | 1216 |
| StyD4I | CCNGG | 4 cut(s) | 115, 758, 827, 2287 |
| StyI | CCWWGG | 1 cut(s) | 1733 |
| TaiI | ACGT | 3 cut(s) | 168, 223, 1244 |
| TaqI | TCGA | 3 cut(s) | 9, 1306, 2213 |
| TatI | WGTACW | 3 cut(s) | 252, 729, 1095 |
| TauI | GCSGC | 1 cut(s) | 231 |
| TfiI | GAWTC | 6 cut(s) | 616, 964, 1379, 1442, 1811, 2036 |
| Tru1I | TTAA | 6 cut(s) | 375, 810, 1100, 1458, 1656, 2184 |
| Tru9I | TTAA | 6 cut(s) | 375, 810, 1100, 1458, 1656, 2184 |
| TscAI | CASTG | 7 cut(s) | 44, 753, 943, 1927, 2224, 2377, 2434 |
| TseFI | GTSAC | 6 cut(s) | 35, 332, 500, 563, 1754, 2219 |
| TseI | GCWGC | 7 cut(s) | 487, 490, 1660, 1886, 1955, 2207, 2258 |
| Tsp45I | GTSAC | 6 cut(s) | 35, 332, 500, 563, 1754, 2219 |
| TspDTI | ATGAA | 5 cut(s) | 341, 1088, 1288, 1815, 1919 |
| TspGWI | ACGGA | 2 cut(s) | 437, 1023 |
| TspRI | CASTG | 7 cut(s) | 44, 753, 943, 1927, 2224, 2377, 2434 |
| VpaK11BI | GGWCC | 3 cut(s) | 88, 756, 1552 |
| XapI | RAATTY | 2 cut(s) | 392, 669 |
| XbaI | TCTAGA | 1 cut(s) | 1979 |
| XceI | RCATGY | 2 cut(s) | 510, 549 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2236 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1120 |
| XspI | CTAG | 7 cut(s) | 1403, 1497, 1595, 1719, 1980, 2079, 2378 |
| ZrmI | AGTACT | 1 cut(s) | 1097 |
| Zsp2I | ATGCAT | 1 cut(s) | 793 |