Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Forward (+)

30114095 .. 30117611

Gene Structure 3517 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1172700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGACGATCAATCAGACGGCGGGGAAGGCAACTCCCAAAGAAATCCGAAGCTGCCGGTACCCGGAAAACGCAACATACTCATCACAAGTGCCCTCCCTTATGTCAACAACATCCCTCACCTTGGCACCATCATTGGCTGTGTTCTAAGTGCTGATGTTTACGCTCGATACTGTCGCCTTCGCGGGTACAATGCGATATACATATGTGGGACTGATGAGTATGGGACTGCAACCGAGACAAAAGCTAGGGAGGAAAATTGTACTCCACAGCAAATTTGTGACAAATACTATGAAATTCATAAACAGGTCTACGAGTGGTTCGATATAAGTTTTGATAAATTCGGACGCACGTCAACTCCTCAACAGACAGAAATTTGCCAACATATTTTCAAGAAACTTTTGGAGAACGGATATTTTTCCGAGAACACAATTCAGCAGCTGTTTTGTGAAACATGCGATCGTTTCTTGGCTGACCGGCTAGTGGAAGGCACTTGCCCAACGCTAGATTGCGGCTATGATTCTGCACGAGGAGATCAGTGCGATAAGTGTGGAAAGCTGTTGAATACAACTGAGCTTAAGGATCCTAGATGTAAAGCATGTCAGGCACGGCCATGCATACGAGAGACGAACCACTTATTTCTAGAGCTCCCTTTACTGAAGGATAAAGTGGAAGAATTTGTCAACAACCTGTCAGTTGAAGGATCTTGGAGCCCGAACGCTATTCAAACTACATACGCATGGCTTAGAGAAGGACTTAAGGTGCGATGTATGACGAGGGACCTTAAGTGGGGTGTTCCCGTTCCACTCGAAAAATTTGAGGACAAGGTTTTTTATGTATGGTTTGATGCGCCTATCGGTTATATTTCTATCACTTCATGCTACACGCCCGATTGGGAGAAGTGGTGGAAGAACCCCAAAAATGTTGAGCTGTACCAGTTCATGGGCAAGGACAATGTGCCATTCCACACGGTTATGTTTCCATCTACGCTTCTTGGAACCGGAGAGAATTGGACACTGATGAAGAGCCTTAGCGTCACAGAATATCTGATGTATGAAACAGGGAAGTTTTCCAAGAGTAGGGGCATAGGAATTTTCGGCAATGATGTGCAAGAAACAAACATTCCCTCAGAAGTGTGGCGATATTATTTGCTAGCTAACCGGCCAGAGGTCTCAGACACCGTTTTTACATGGGCGGACTTACAAGCAAAACTAAACACTGAGTTGCTGAATAATTTGGGAAATTTCATTAACCGGGTATTGAGCTTCATTGCAAAACCTCAAGGACAAGGATATGGATCTATTATTCCAGACGCTTCGGGGGCAGAATCACATTCCTTGACGAAAAGATTGGCAGAGAGAATCGGCATATATGTGGATCAATACATAGAAGCAATGGAGAAGGTCAAGCTAAAGCAAGCACTGAAAATTGCAATGAGCATATCCAGTGAGGGAAATGCATATTTACAAGAGAACCAGTTCTGGAAACTTTACAAACAAGATCAAGCTTCCTGCTGTATTGTCATCAAGACTTCTGCAGGACTCGTTTATCTTCTCGCGTGTGTGCTGGAACCTTTCATGCCTTCATTTTCTCTCAAGGTGCTAAAGCAGCTCAATTTGCCACCCGAAAATCAGTTTTCGCTTTGCAACGAGGATGTTGAAAGGGCAAGAAAGCCTTGGGAAATATTACCTGATGGTCACAAGATTGGGACACCAGAGCCTTTGTTCAAAGAATTGAAAGATGAAGACGTGGAGTTCTTTAGGAGCAAATTTTCTGGAAGCCAAGCTGAAAGGGTTGCTAAGGGAGAGGCTGAAGCAAAGAAAATGGCTGAGAAATTGGAGCAAACAAAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

tRNA-synt_1g tRNA_bind

Protein Analysis

618

Amino Acids

70.47

Weight (kDa)

5.69

Isoelectric Point (pI)

33.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	26 - 420	3.5e-151	tRNA synthetases class I (M)
tRNA-synt_1	PF00133	27 - 269	6.4e-08	tRNA synthetases class I (I, L, M and V)
Anticodon_3	PF19303	441 - 594	8.5e-21	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	58
AccB1I	GGYRCC	2 cut(s)	58, 125
AccB7I	CCANNNNNTGG	1 cut(s)	940
AccI	GTMKAC	1 cut(s)	309
AccII	CGCG	2 cut(s)	183, 1556
AciI	CCGC	4 cut(s)	21, 183, 510, 1193
AclWI	GGATC	5 cut(s)	575, 588, 709, 1303, 1383
AcoI	YGGCCR	2 cut(s)	608, 1160
AcuI	CTGAAG	2 cut(s)	677, 1830
AfaI	GTAC	4 cut(s)	60, 188, 262, 932
AfiI	CCNNNNNNNGG	7 cut(s)	62, 122, 481, 787, 940, 1077, 1165
AflII	CTTAAG	3 cut(s)	575, 755, 782
AgsI	TTSAA	7 cut(s)	391, 562, 698, 725, 1658, 1726, 1735
AjiI	CACGTC	2 cut(s)	351, 1747
Alw21I	GWGCWC	1 cut(s)	648
Alw26I	GTCTC	3 cut(s)	230, 617, 1174
AlwI	GGATC	5 cut(s)	575, 588, 709, 1303, 1383
AlwNI	CAGNNNCTG	1 cut(s)	439
AoxI	GGCC	2 cut(s)	608, 1160
ApeKI	GCWGC	3 cut(s)	52, 436, 1606
Asp700I	GAANNNNTTC	1 cut(s)	1475
Asp718I	GGTACC	1 cut(s)	58
AspLEI	GCGC	1 cut(s)	850
AspS9I	GGNCC	1 cut(s)	778
AsuC2I	CCSGG	2 cut(s)	63, 1253
AsuHPI	GGTGA	1 cut(s)	110
AsuNHI	GCTAGC	1 cut(s)	1150
AvaII	GGWCC	1 cut(s)	778
BaeGI	GKGCMC	1 cut(s)	94
BamHI	GGATCC	1 cut(s)	580
BanI	GGYRCC	2 cut(s)	58, 125
BanII	GRGCYC	2 cut(s)	648, 713
BauI	CACGAG	1 cut(s)	525
BbsI	GAAGAC	1 cut(s)	1749
Bbv12I	GWGCWC	1 cut(s)	648
BbvI	GCAGC	3 cut(s)	39, 448, 1618
BccI	CCATC	3 cut(s)	137, 988, 1685
BceAI	ACGGC	2 cut(s)	34, 623
BcnI	CCSGG	2 cut(s)	63, 1253
BcoDI	GTCTC	3 cut(s)	230, 617, 1174
BfaI	CTAG	6 cut(s)	246, 479, 503, 585, 641, 1151
BfmI	CTRYAG	1 cut(s)	1533
BfrI	CTTAAG	3 cut(s)	575, 755, 782
BisI	GCNGC	4 cut(s)	53, 437, 511, 1607
BlsI	GCNGC	4 cut(s)	54, 438, 512, 1608
Bme1390I	CCNGG	2 cut(s)	63, 1253
Bme18I	GGWCC	1 cut(s)	778
BmgBI	CACGTC	2 cut(s)	351, 1747
BmgT120I	GGNCC	1 cut(s)	778
BmiI	GGNNCC	7 cut(s)	60, 127, 582, 710, 779, 997, 1569
BmrFI	CCNGG	2 cut(s)	63, 1253
BmsI	GCATC	1 cut(s)	835
BmtI	GCTAGC	1 cut(s)	1154
BoxI	GACNNNNGTC	1 cut(s)	349
BpiI	GAAGAC	1 cut(s)	1749
Bpu10I	CCTNAGC	2 cut(s)	1028, 1797
BpuEI	CTTGAG	2 cut(s)	1263, 1577
BpuMI	CCSGG	2 cut(s)	63, 1253
BsaBI	GATNNNNATC	1 cut(s)	1294
BsaI	GGTCTC	1 cut(s)	1174
BsaJI	CCNNGG	2 cut(s)	121, 1673
BsaWI	WCCGGW	1 cut(s)	998
BsaXI	ACNNNNNCTCC	4 cut(s)	340, 370, 887, 917
Bsc4I	CCNNNNNNNGG	7 cut(s)	62, 122, 481, 787, 940, 1077, 1165
Bse118I	RCCGGY	3 cut(s)	55, 474, 1158
Bse1I	ACTGG	3 cut(s)	934, 1443, 1474
Bse3DI	GCAATG	4 cut(s)	1105, 1266, 1398, 1437
Bse8I	GATNNNNATC	1 cut(s)	1294
BseDI	CCNNGG	2 cut(s)	121, 1673
BseGI	GGATG	2 cut(s)	111, 1657
BseJI	GATNNNNATC	1 cut(s)	1294
BseLI	CCNNNNNNNGG	7 cut(s)	62, 122, 481, 787, 940, 1077, 1165
BseMI	GCAATG	4 cut(s)	1105, 1266, 1398, 1437
BseMII	CTCAG	5 cut(s)	561, 1140, 1185, 1209, 1818
BseNI	ACTGG	3 cut(s)	934, 1443, 1474
BseRI	GAGGAG	2 cut(s)	348, 543
BseSI	GKGCMC	1 cut(s)	94
BseXI	GCAGC	3 cut(s)	39, 448, 1618
BsgI	GTGCAG	1 cut(s)	507
Bsh1236I	CGCG	2 cut(s)	183, 1556
Bsh1285I	CGRYCG	1 cut(s)	460
BshFI	GGCC	2 cut(s)	610, 1162
BshNI	GGYRCC	2 cut(s)	58, 125
BsiEI	CGRYCG	1 cut(s)	460
BsiHKAI	GWGCWC	1 cut(s)	648
BsiSI	CCGG	6 cut(s)	56, 63, 475, 999, 1159, 1252
BslFI	GGGAC	4 cut(s)	223, 238, 791, 1720
BslI	CCNNNNNNNGG	7 cut(s)	62, 122, 481, 787, 940, 1077, 1165
BsmAI	GTCTC	3 cut(s)	230, 617, 1174
BsmBI	CGTCTC	1 cut(s)	617
BsmFI	GGGAC	4 cut(s)	223, 238, 791, 1720
BsnI	GGCC	2 cut(s)	610, 1162
Bso31I	GGTCTC	1 cut(s)	1174
Bsp1286I	GDGCHC	3 cut(s)	94, 648, 713
Bsp143I	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
BspACI	CCGC	4 cut(s)	21, 183, 510, 1193
BspANI	GGCC	2 cut(s)	610, 1162
BspCNI	CTCAG	5 cut(s)	562, 1139, 1184, 1210, 1819
BspFNI	CGCG	2 cut(s)	183, 1556
BspLI	GGNNCC	7 cut(s)	60, 127, 582, 710, 779, 997, 1569
BspMAI	CTGCAG	1 cut(s)	1537
BspOI	GCTAGC	1 cut(s)	1154
BspPI	GGATC	5 cut(s)	575, 588, 709, 1303, 1383
BspQI	GCTCTTC	1 cut(s)	1016
BspT107I	GGYRCC	2 cut(s)	58, 125
BspTI	CTTAAG	3 cut(s)	575, 755, 782
BspTNI	GGTCTC	1 cut(s)	1174
BsrDI	GCAATG	4 cut(s)	1105, 1266, 1398, 1437
BsrFI	RCCGGY	3 cut(s)	55, 474, 1158
BsrI	ACTGG	3 cut(s)	934, 1443, 1474
BssAI	RCCGGY	3 cut(s)	55, 474, 1158
BssECI	CCNNGG	2 cut(s)	121, 1673
BssMI	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
BssSI	CACGAG	1 cut(s)	525
BssT1I	CCWWGG	2 cut(s)	121, 1673
Bst2BI	CACGAG	1 cut(s)	525
Bst4CI	ACNGT	3 cut(s)	173, 970, 1180
Bst6I	CTCTTC	1 cut(s)	1016
BstAFI	CTTAAG	3 cut(s)	575, 755, 782
BstC8I	GCNNGC	2 cut(s)	1152, 1416
BstDEI	CTNAG	9 cut(s)	146, 570, 743, 1028, 1126, 1171, 1218, 1797, 1827
BstF5I	GGATG	2 cut(s)	111, 1657
BstFNI	CGCG	2 cut(s)	183, 1556
BstHHI	GCGC	1 cut(s)	850
BstKTI	GATC	8 cut(s)	10, 460, 535, 583, 704, 1298, 1378, 1501
BstMAI	GTCTC	3 cut(s)	230, 617, 1174
BstMBI	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
BstMCI	CGRYCG	1 cut(s)	460
BstMWI	GCNNNNNNNGC	5 cut(s)	27, 602, 1319, 1606, 1615
BstNSI	RCATGY	2 cut(s)	456, 600
BstPAI	GACNNNNGTC	1 cut(s)	349
BstSCI	CCNGG	2 cut(s)	61, 1251
BstSFI	CTRYAG	1 cut(s)	1533
BstSLI	GKGCMC	1 cut(s)	94
BstUI	CGCG	2 cut(s)	183, 1556
BstV1I	GCAGC	3 cut(s)	39, 448, 1618
BstV2I	GAAGAC	1 cut(s)	1749
BstX2I	RGATCY	3 cut(s)	580, 701, 1295
BstYI	RGATCY	3 cut(s)	580, 701, 1295
BsuRI	GGCC	2 cut(s)	610, 1162
BtgZI	GCGATG	1 cut(s)	778
BtrI	CACGTC	2 cut(s)	351, 1747
BtsCI	GGATG	2 cut(s)	111, 1657
BtsIMutI	CAGTG	5 cut(s)	542, 1013, 1215, 1418, 1450
Cac8I	GCNNGC	2 cut(s)	1152, 1416
CaiI	CAGNNNCTG	1 cut(s)	439
CfoI	GCGC	1 cut(s)	850
Cfr10I	RCCGGY	3 cut(s)	55, 474, 1158
Cfr13I	GGNCC	1 cut(s)	778
CseI	GACGC	3 cut(s)	354, 1021, 1319
Csp6I	GTAC	4 cut(s)	59, 187, 261, 931
CviAII	CATG	8 cut(s)	453, 597, 612, 738, 876, 940, 1188, 1576
CviQI	GTAC	4 cut(s)	59, 187, 261, 931
DdeI	CTNAG	9 cut(s)	146, 570, 743, 1028, 1126, 1171, 1218, 1797, 1827
DpnI	GATC	8 cut(s)	9, 459, 534, 582, 703, 1297, 1377, 1500
DpnII	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
EaeI	YGGCCR	2 cut(s)	608, 1160
Eam1104I	CTCTTC	1 cut(s)	1016
EarI	CTCTTC	1 cut(s)	1016
EciI	GGCGGA	1 cut(s)	1208
Ecl136II	GAGCTC	1 cut(s)	646
Eco130I	CCWWGG	2 cut(s)	121, 1673
Eco24I	GRGCYC	2 cut(s)	648, 713
Eco31I	GGTCTC	1 cut(s)	1174
Eco47I	GGWCC	1 cut(s)	778
Eco53kI	GAGCTC	1 cut(s)	646
Eco57I	CTGAAG	2 cut(s)	677, 1830
EcoICRI	GAGCTC	1 cut(s)	646
EcoO109I	RGGNCCY	1 cut(s)	778
EcoT14I	CCWWGG	2 cut(s)	121, 1673
EcoT22I	ATGCAT	2 cut(s)	617, 1459
EcoT38I	GRGCYC	2 cut(s)	648, 713
ErhI	CCWWGG	2 cut(s)	121, 1673
Esp3I	CGTCTC	1 cut(s)	617
FaeI	CATG	8 cut(s)	456, 600, 615, 741, 879, 943, 1191, 1579
FalI	AAGNNNNNCTT	2 cut(s)	1657, 1689
FaqI	GGGAC	4 cut(s)	223, 238, 791, 1720
FatI	CATG	8 cut(s)	452, 596, 611, 737, 875, 939, 1187, 1575
FauI	CCCGC	2 cut(s)	14, 176
FauNDI	CATATG	1 cut(s)	203
FblI	GTMKAC	1 cut(s)	309
Fnu4HI	GCNGC	4 cut(s)	53, 437, 511, 1607
FokI	GGATG	2 cut(s)	98, 1664
FriOI	GRGCYC	2 cut(s)	648, 713
Fsp4HI	GCNGC	4 cut(s)	53, 437, 511, 1607
FspBI	CTAG	6 cut(s)	246, 479, 503, 585, 641, 1151
GlaI	GCGC	1 cut(s)	849
GluI	GCNGC	4 cut(s)	53, 437, 511, 1607
HaeIII	GGCC	2 cut(s)	610, 1162
HapII	CCGG	6 cut(s)	56, 63, 475, 999, 1159, 1252
HgaI	GACGC	3 cut(s)	354, 1021, 1319
HhaI	GCGC	1 cut(s)	850
Hin1II	CATG	8 cut(s)	456, 600, 615, 741, 879, 943, 1191, 1579
Hin6I	GCGC	1 cut(s)	848
HinP1I	GCGC	1 cut(s)	848
HincII	GTYRAC	3 cut(s)	106, 354, 682
HindII	GTYRAC	3 cut(s)	106, 354, 682
HindIII	AAGCTT	1 cut(s)	1503
HinfI	GANTC	4 cut(s)	518, 1325, 1359, 1539
HpaII	CCGG	6 cut(s)	56, 63, 475, 999, 1159, 1252
HphI	GGTGA	1 cut(s)	110
Hpy166II	GTNNAC	5 cut(s)	106, 160, 310, 354, 682
Hpy188I	TCNGA	7 cut(s)	16, 48, 344, 421, 1047, 1129, 1174
Hpy188III	TCNNGA	6 cut(s)	391, 641, 1307, 1480, 1525, 1773
Hpy8I	GTNNAC	5 cut(s)	106, 160, 310, 354, 682
HpyAV	CCTTC	8 cut(s)	20, 188, 479, 652, 692, 743, 1393, 1590
HpyCH4III	ACNGT	3 cut(s)	173, 970, 1180
HpyCH4IV	ACGT	2 cut(s)	350, 1746
HpyCH4V	TGCA	9 cut(s)	230, 524, 615, 1108, 1271, 1430, 1457, 1535, 1644
HpyF10VI	GCNNNNNNNGC	5 cut(s)	27, 602, 1319, 1606, 1615
HpyF3I	CTNAG	9 cut(s)	146, 570, 743, 1028, 1126, 1171, 1218, 1797, 1827
HpySE526I	ACGT	2 cut(s)	350, 1746
Hsp92II	CATG	8 cut(s)	456, 600, 615, 741, 879, 943, 1191, 1579
HspAI	GCGC	1 cut(s)	848
KpnI	GGTACC	1 cut(s)	62
Kzo9I	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
LguI	GCTCTTC	1 cut(s)	1016
LmnI	GCTCC	4 cut(s)	651, 708, 1761, 1837
Lsp1109I	GCAGC	3 cut(s)	39, 448, 1618
LweI	GCATC	1 cut(s)	835
MaeI	CTAG	6 cut(s)	246, 479, 503, 585, 641, 1151
MaeII	ACGT	2 cut(s)	350, 1746
MaeIII	GTNAC	3 cut(s)	278, 1033, 1694
MalI	GATC	8 cut(s)	9, 459, 534, 582, 703, 1297, 1377, 1500
MboI	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
MboII	GAAGA	5 cut(s)	683, 919, 1033, 1541, 1754
MflI	RGATCY	3 cut(s)	580, 701, 1295
MhlI	GDGCHC	3 cut(s)	94, 648, 713
MlyI	GAGTC	1 cut(s)	1533
Mph1103I	ATGCAT	2 cut(s)	617, 1459
MroXI	GAANNNNTTC	1 cut(s)	1475
MseI	TTAA	4 cut(s)	576, 756, 783, 1248
MslI	CAYNNNNRTG	4 cut(s)	610, 736, 971, 1370
MspA1I	CMGCKG	1 cut(s)	439
MspCI	CTTAAG	3 cut(s)	575, 755, 782
MspI	CCGG	6 cut(s)	56, 63, 475, 999, 1159, 1252
MspR9I	CCNGG	2 cut(s)	63, 1253
MvnI	CGCG	2 cut(s)	183, 1556
MwoI	GCNNNNNNNGC	5 cut(s)	27, 602, 1319, 1606, 1615
NciI	CCSGG	2 cut(s)	63, 1253
NdeI	CATATG	1 cut(s)	203
NdeII	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
NheI	GCTAGC	1 cut(s)	1150
NlaIII	CATG	8 cut(s)	456, 600, 615, 741, 879, 943, 1191, 1579
NlaIV	GGNNCC	7 cut(s)	60, 127, 582, 710, 779, 997, 1569
NmuCI	GTSAC	3 cut(s)	278, 1033, 1694
NsiI	ATGCAT	2 cut(s)	617, 1459
NspI	RCATGY	2 cut(s)	456, 600
PciSI	GCTCTTC	1 cut(s)	1016
PcsI	WCGNNNNNNNCGW	1 cut(s)	318
PdmI	GAANNNNTTC	1 cut(s)	1475
PfeI	GAWTC	3 cut(s)	518, 1325, 1359
PflMI	CCANNNNNTGG	1 cut(s)	940
PkrI	GCNGC	4 cut(s)	54, 438, 512, 1608
Ple19I	CGATCG	1 cut(s)	460
PleI	GAGTC	1 cut(s)	1533
PpsI	GAGTC	1 cut(s)	1533
PpuMI	RGGWCCY	1 cut(s)	778
PshAI	GACNNNNGTC	1 cut(s)	349
Psp124BI	GAGCTC	1 cut(s)	648
Psp5II	RGGWCCY	1 cut(s)	778
PspN4I	GGNNCC	7 cut(s)	60, 127, 582, 710, 779, 997, 1569
PspPI	GGNCC	1 cut(s)	778
PspPPI	RGGWCCY	1 cut(s)	778
PstI	CTGCAG	1 cut(s)	1537
PstNI	CAGNNNCTG	1 cut(s)	439
PsuI	RGATCY	3 cut(s)	580, 701, 1295
PvuI	CGATCG	1 cut(s)	460
PvuII	CAGCTG	1 cut(s)	439
RsaI	GTAC	4 cut(s)	60, 188, 262, 932
RsaNI	GTAC	4 cut(s)	59, 187, 261, 931
RseI	CAYNNNNRTG	4 cut(s)	610, 736, 971, 1370
SacI	GAGCTC	1 cut(s)	648
SapI	GCTCTTC	1 cut(s)	1016
SaqAI	TTAA	4 cut(s)	576, 756, 783, 1248
SatI	GCNGC	4 cut(s)	53, 437, 511, 1607
Sau3AI	GATC	8 cut(s)	7, 457, 532, 580, 701, 1295, 1375, 1498
Sau96I	GGNCC	1 cut(s)	778
SchI	GAGTC	1 cut(s)	1533
ScrFI	CCNGG	2 cut(s)	63, 1253
SduI	GDGCHC	3 cut(s)	94, 648, 713
SfaNI	GCATC	1 cut(s)	835
SfcI	CTRYAG	1 cut(s)	1533
SinI	GGWCC	1 cut(s)	778
SmiMI	CAYNNNNRTG	4 cut(s)	610, 736, 971, 1370
SmlI	CTYRAG	5 cut(s)	575, 755, 782, 1278, 1592
SmoI	CTYRAG	5 cut(s)	575, 755, 782, 1278, 1592
SsiI	CCGC	4 cut(s)	21, 183, 510, 1193
SspI	AATATT	1 cut(s)	1683
SspMI	CTAG	6 cut(s)	246, 479, 503, 585, 641, 1151
SstI	GAGCTC	1 cut(s)	648
StyD4I	CCNGG	2 cut(s)	61, 1251
StyI	CCWWGG	2 cut(s)	121, 1673
TaaI	ACNGT	3 cut(s)	173, 970, 1180
TaiI	ACGT	2 cut(s)	353, 1749
TaqI	TCGA	3 cut(s)	166, 321, 807
TatI	WGTACW	1 cut(s)	260
TauI	GCSGC	1 cut(s)	513
TfiI	GAWTC	3 cut(s)	518, 1325, 1359
Tru1I	TTAA	4 cut(s)	576, 756, 783, 1248
Tru9I	TTAA	4 cut(s)	576, 756, 783, 1248
TscAI	CASTG	5 cut(s)	542, 1020, 1222, 1425, 1450
TseFI	GTSAC	3 cut(s)	278, 1033, 1694
TseI	GCWGC	3 cut(s)	52, 436, 1606
Tsp45I	GTSAC	3 cut(s)	278, 1033, 1694
TspGWI	ACGGA	1 cut(s)	423
TspRI	CASTG	5 cut(s)	542, 1020, 1222, 1425, 1450
Van91I	CCANNNNNTGG	1 cut(s)	940
Vha464I	CTTAAG	3 cut(s)	575, 755, 782
VpaK11BI	GGWCC	1 cut(s)	778
XbaI	TCTAGA	1 cut(s)	640
XceI	RCATGY	2 cut(s)	456, 600
XmiI	GTMKAC	1 cut(s)	309
XmnI	GAANNNNTTC	1 cut(s)	1475
XspI	CTAG	6 cut(s)	246, 479, 503, 585, 641, 1151
Zsp2I	ATGCAT	2 cut(s)	617, 1459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.