Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Forward (+)

25159273 .. 25160557

Gene Structure 1285 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1161000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 312 bp

ATGGAGAAGGTCAAGCTAAAGCAAGCACTGAAAATTGCAATGAGCGTATCCAGTGAAGGCAATACATATTTACAAGAGAGCAAGTTCTGGCAACTTTACAAACAGGATCAAGCTTCCTGCTCTCTTGTCATCAAAACTTCTGCAGGACTCATTTATCTTCTCGCGTGCTTGCCGGAACCTTTCATTCCTTCATTTTCTCTCAAGGTGCTAAAACAGCTCAATTTGCCAACCGAAAATCAGTTTTTGCTTTGCGATAAGGATGTTGAAAGGGCAAGAAAGCCTTTGGATATTACCTGCTGTTCACAAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

tRNA-synt_1g tRNA_bind

Protein Analysis

104

Amino Acids

11.67

Weight (kDa)

8.28

Isoelectric Point (pI)

48.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Anticodon_3	PF19303	1 - 76	9.6e-12	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	302
AccII	CGCG	1 cut(s)	164
AclWI	GGATC	1 cut(s)	114
AgsI	TTSAA	1 cut(s)	266
AluBI	AGCT	3 cut(s)	16, 113, 217
AluI	AGCT	3 cut(s)	16, 113, 217
AlwI	GGATC	1 cut(s)	114
BciVI	GTATCC	1 cut(s)	58
BfmI	CTRYAG	1 cut(s)	141
BfuAI	ACCTGC	1 cut(s)	302
BfuI	GTATCC	1 cut(s)	58
BmiI	GGNNCC	1 cut(s)	177
BpuEI	CTTGAG	1 cut(s)	185
Bse1I	ACTGG	1 cut(s)	51
Bse3DI	GCAATG	1 cut(s)	45
BseGI	GGATG	1 cut(s)	265
BseMI	GCAATG	1 cut(s)	45
BseNI	ACTGG	1 cut(s)	51
Bsh1236I	CGCG	1 cut(s)	164
BsiSI	CCGG	1 cut(s)	173
Bsp143I	GATC	1 cut(s)	106
BspFNI	CGCG	1 cut(s)	164
BspLI	GGNNCC	1 cut(s)	177
BspMAI	CTGCAG	1 cut(s)	145
BspMI	ACCTGC	1 cut(s)	302
BspPI	GGATC	1 cut(s)	114
BsrDI	GCAATG	1 cut(s)	45
BsrI	ACTGG	1 cut(s)	51
BssMI	GATC	1 cut(s)	106
BstC8I	GCNNGC	3 cut(s)	24, 166, 170
BstF5I	GGATG	1 cut(s)	265
BstFNI	CGCG	1 cut(s)	164
BstKTI	GATC	1 cut(s)	109
BstMBI	GATC	1 cut(s)	106
BstMWI	GCNNNNNNNGC	2 cut(s)	214, 223
BstSFI	CTRYAG	1 cut(s)	141
BstUI	CGCG	1 cut(s)	164
BsuI	GTATCC	1 cut(s)	58
BtsCI	GGATG	1 cut(s)	265
BtsIMutI	CAGTG	2 cut(s)	26, 58
BveI	ACCTGC	1 cut(s)	302
Cac8I	GCNNGC	3 cut(s)	24, 166, 170
CviJI	RGCY	4 cut(s)	16, 113, 217, 280
CviKI_1	RGCY	4 cut(s)	16, 113, 217, 280
DpnI	GATC	1 cut(s)	108
DpnII	GATC	1 cut(s)	106
FaiI	YATR	1 cut(s)	67
FalI	AAGNNNNNCTT	2 cut(s)	265, 297
FokI	GGATG	1 cut(s)	272
HapII	CCGG	1 cut(s)	173
HindIII	AAGCTT	1 cut(s)	111
HinfI	GANTC	1 cut(s)	147
HpaII	CCGG	1 cut(s)	173
Hpy166II	GTNNAC	1 cut(s)	302
Hpy8I	GTNNAC	1 cut(s)	302
HpyAV	CCTTC	2 cut(s)	50, 198
HpyCH4V	TGCA	2 cut(s)	38, 143
HpyF10VI	GCNNNNNNNGC	2 cut(s)	214, 223
Kzo9I	GATC	1 cut(s)	106
LpnPI	CCDG	7 cut(s)	64, 73, 89, 129, 130, 186, 307
MalI	GATC	1 cut(s)	108
MboI	GATC	1 cut(s)	106
MboII	GAAGA	1 cut(s)	149
MluCI	AATT	2 cut(s)	33, 220
MlyI	GAGTC	1 cut(s)	141
MspI	CCGG	1 cut(s)	173
MvnI	CGCG	1 cut(s)	164
MwoI	GCNNNNNNNGC	2 cut(s)	214, 223
NdeII	GATC	1 cut(s)	106
NlaIV	GGNNCC	1 cut(s)	177
PleI	GAGTC	1 cut(s)	141
PpsI	GAGTC	1 cut(s)	141
PspN4I	GGNNCC	1 cut(s)	177
PstI	CTGCAG	1 cut(s)	145
Sau3AI	GATC	1 cut(s)	106
SchI	GAGTC	1 cut(s)	141
SetI	ASST	7 cut(s)	12, 18, 115, 181, 207, 219, 296
SfcI	CTRYAG	1 cut(s)	141
SmlI	CTYRAG	1 cut(s)	200
SmoI	CTYRAG	1 cut(s)	200
Sse9I	AATT	2 cut(s)	33, 220
TasI	AATT	2 cut(s)	33, 220
TscAI	CASTG	2 cut(s)	33, 58
TspDTI	ATGAA	2 cut(s)	172, 180
TspRI	CASTG	2 cut(s)	33, 58

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.