Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001029

Physical Location & Seq

Genomic Coordinates Reverse (-)

77209760 .. 77211558

Gene Structure 1799 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021676

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGGAGACCTCGCCAAGCAGGACCAGAGAGTCCCCAAGCTCCCCATCCCGGGGAAGCGAAACATCCTGATCACTAGTGCCTTGCCATATGTTAACAACGTCCCCCACCTGGGGAACATCATTGGCTGCGTCCTGAGCGCCGATGTCTTTGCTCGGTACTGCCGTCTCCGGGGATACAATACCGTCTATATCTCCGGGACCGATGAGTATGGCACAGCGACGGAGACCAAAGCCATGGAAGAGAAGTGCAGCCCCCAAGAGATATGTGACAAGTATCACAAAATCCATAGGGAGGTTTATGAGTGGTTCAACATAAGCTTCGACCAATTCGGCCGAACTTCCACGCCGCAGCAAACTGAGATTCTTAGCGGACCGATTCGTGGAGGGGACATGCCCTACTCAAGGATATGTTACTATGATTCTGCTCGAGGAGACCAATGTGACAAGTGTGGAAAGCTTTTGAATCCCACGGAACTGAAAGATCCTAAATGCAAGGTATGCAAGGAAACTCCACGGATTCGCAAAACAAATCACTTGTTTCTGGAGCTCCCTTTGCTCAAGGATAAGTTGGAAGAGTACATCAATAAGACATCAGTGGAAGGGTGTTGGAGTCAGAATGCGGTTCAAATCACAAGTGCCTGGCTCAAAGAGGGGCTTAAACAACGTTGCATTACAAGGGATCTCAAGTGGGGTGTTCCGGTTCCACACGATGGCTTCAGGGACAAGGTATTCTACGTGTGGTTCGATGCTCCTATTGGATACATCTCCATTACTAAGTGTTACACTCCTGATCACTGGGAGAAGTGGTGGAAGAATCCTGAGGACGTCGAATTGTATCAGTTTATGGGCAAGGACAATGTGCCATTTCACACGGTCATGTTTCCTTCCGCGCTTCTTGGAACTGGAGAAAATTGGACATTCATGAAGAACATTAGTGTCACTGAATACTTGAACTATGAAGAAGGCAAGTTTTCCAAGAGTAGAGGCATTGGAGTTTTTGGAAACGATGCAAAAGGAACGGGCATTCCAGTTGAAGTTTGGCGTTATTACCTGCTGACTAATAGGCCTGAGGTATCAGACACATTGTTCACCTGGGCCGATTTACAAGCAAAACTCAAAAGTGAGTTGGTGGGCAACTTGGGCAACTTCATCAACAGAGTTTTGAGTTTCGTTGCCAAACCTTCAGCCAAAGGAGGATACGGTGACACCATTCCTGATGCTCCACATGCAGACTCTGATGCCTTGACACTTGAATTAGCAGAAATAGTCGGCAGATACGTCGAGCAATACATAGAAGCAATGGAGAAGCTCAAACTAAAGCAAGGGCTGAAAATAGCCATGAGCATTTCTAGCGAGGGGAATGCATATCTCCAAGAATCCAAGTTCTGGAAGCTCTACAAGGAGGACCGACCTCGGTGCTCCATTGTCATTAAAACTTCAGTAGGGTTAGTGCATCTTCTTGCTTGCTTATTGGAACCTTACATGCCCTCATTTTCTGCTAGAGTACTTGAGCAGCTAAACTTGCCTCCTGAGTCGATCAAATCCCTTTGTGATGTCAATAAAGCAAAACGGCCCTGGGAGATCGTACCTGATGGTCACAAGATTGGAGAACCCAAGACATTGTTCACGACGTTGAACGGCGAAGAAGTCGAATCTCTGCGGAAAAAGTTTTCTGGAAGCCAAGCTGATAGGGAAAAACGTACACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016070 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0042221 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046686 GO:0050896 GO:0071704 GO:0090304 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00450 ko00970 map00450 map00970

Pfam Domains

Protein Families

Anticodon_1 tRNA-synt_1g tRNA_bind

Protein Analysis

570

Amino Acids

64.8

Weight (kDa)

8.19

Isoelectric Point (pI)

35.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1g	PF09334	22 - 121	3.3e-37	tRNA synthetases class I (M)
tRNA-synt_1g	PF09334	136 - 388	3.8e-94	tRNA synthetases class I (M)
Anticodon_3	PF19303	418 - 561	1.1e-18	Anticodon binding domain of methionyl tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13780
fragaria_vesca	FvH4_4g13560 FvH4_4g13560 FvH4_4g13560 FvH4_4g36101 FvH4_4g36103
malus_domestica	MD04G1013400.v1.1 MD05G1172700.v1.1 MD10G1161000.v1.1
prunus_persica	Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.1G159500_v2.0.a1 Prupe.2G130000_v2.0.a1 Prupe.2G130800_v2.0.a1 Prupe.8G200100_v2.0.a1 Prupe.8G200100_v2.0.a1
pyrus_communis	pycom04g01040 pycom05g15930 pycom10g13910
rosa_chinensis	RchiOBHm_Chr1g0332221 RchiOBHm_Chr2g0166871 RchiOBHm_Chr4g0414311 RchiOBHm_Chr4g0442441
rosa_laevigata	RLG00000005711 RLG00000008159 RLG00000021676
rosa_multiflora	Rmu_co8251943.1_g000001 Rmu_co8341625.1_g000001 Rmu_sc0000733.1_g000008 Rmu_sc0001063.1_g000003 Rmu_sc0002735.1_g000014 Rmu_sc0005196.1_g000006 Rmu_sc0005344.1_g000007 Rmu_sc0032860.1_g000001
rosa_roxburghii	Rroxscaffold_2G00084040 Rroxscaffold_2G00084460 Rroxscaffold_4G00318360 Rroxscaffold_5G00358180
rosa_rugosa	Rorug01G0098500 Rorug01G0098600 Rorug01G0098700 Rorug01G0098800 Rorug01G0098900 Rorug02G0526100 Rorug04G0126800
rosa_samantha	Rh1AG122600 Rh1BG093900 Rh1CG117200 Rh1DG128200 Rh2AG593100 Rh2BG604400 Rh2CG575300 Rh2DG616000 Rh4AG184800 Rh4BG184300 Rh4CG197700 Rh4DG181400 Rh4DG182000
rosa_wichuraiana	Rw1G010000 Rw2G049370 Rw4G015810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	29
AatII	GACGTC	1 cut(s)	828
Acc36I	ACCTGC	1 cut(s)	1059
AccB7I	CCANNNNNTGG	2 cut(s)	710, 1386
AccII	CGCG	1 cut(s)	890
AciI	CCGC	5 cut(s)	347, 369, 620, 888, 1660
AclI	AACGTT	1 cut(s)	664
AclWI	GGATC	2 cut(s)	476, 687
AcoI	YGGCCR	1 cut(s)	331
AcuI	CTGAAG	3 cut(s)	700, 1167, 1422
AcyI	GRCGYC	1 cut(s)	825
AfaI	GTAC	5 cut(s)	158, 578, 1506, 1587, 1702
AflIII	ACRYGT	1 cut(s)	735
AgsI	TTSAA	7 cut(s)	310, 463, 626, 952, 1034, 1253, 1636
AhlI	ACTAGT	1 cut(s)	74
AjnI	CCWGG	4 cut(s)	108, 638, 1091, 1574
AjuI	GAANNNNNNNTTGG	2 cut(s)	1607, 1639
AloI	GAACNNNNNNTCC	2 cut(s)	1009, 1041
AluBI	AGCT	8 cut(s)	40, 318, 457, 547, 1309, 1393, 1516, 1685
AluI	AGCT	8 cut(s)	40, 318, 457, 547, 1309, 1393, 1516, 1685
Alw21I	GWGCWC	2 cut(s)	549, 1421
Alw26I	GTCTC	3 cut(s)	170, 218, 426
AlwI	GGATC	2 cut(s)	476, 687
AlwNI	CAGNNNCTG	1 cut(s)	1235
Ama87I	CYCGRG	2 cut(s)	49, 426
AoxI	GGCC	4 cut(s)	331, 1064, 1095, 1571
ApeKI	GCWGC	4 cut(s)	126, 249, 349, 1513
AspLEI	GCGC	2 cut(s)	140, 892
AspS9I	GGNCC	6 cut(s)	22, 198, 371, 1095, 1405, 1572
AsuC2I	CCSGG	4 cut(s)	50, 51, 170, 196
AsuHPI	GGTGA	2 cut(s)	1081, 1214
AvaI	CYCGRG	2 cut(s)	49, 426
AvaII	GGWCC	4 cut(s)	22, 198, 371, 1405
AxyI	CCTNAGG	2 cut(s)	819, 1068
BaeI	ACNNNNGTAYC	2 cut(s)	148, 181
BanII	GRGCYC	1 cut(s)	549
Bbv12I	GWGCWC	2 cut(s)	549, 1421
BbvI	GCAGC	4 cut(s)	113, 261, 361, 1525
BccI	CCATC	3 cut(s)	53, 704, 1586
BceAI	ACGGC	3 cut(s)	147, 1586, 1654
BcgI	CGANNNNNNTGC	8 cut(s)	131, 165, 1261, 1295, 1343, 1377, 1398, 1432
BciT130I	CCWGG	4 cut(s)	110, 640, 1093, 1576
BciVI	GTATCC	3 cut(s)	167, 752, 1190
BclI	TGATCA	2 cut(s)	69, 790
BcnI	CCSGG	4 cut(s)	50, 51, 170, 196
BcoDI	GTCTC	3 cut(s)	170, 218, 426
BcuI	ACTAGT	1 cut(s)	74
BfaI	CTAG	3 cut(s)	75, 1350, 1500
BfoI	RGCGCY	1 cut(s)	141
BfuAI	ACCTGC	1 cut(s)	1059
BfuI	GTATCC	3 cut(s)	167, 752, 1190
BisI	GCNGC	5 cut(s)	127, 250, 347, 350, 1514
BlsI	GCNGC	5 cut(s)	128, 251, 348, 351, 1515
BmcAI	AGTACT	1 cut(s)	1506
Bme1390I	CCNGG	8 cut(s)	50, 51, 110, 170, 196, 640, 1093, 1576
Bme18I	GGWCC	4 cut(s)	22, 198, 371, 1405
BmeT110I	CYCGRG	2 cut(s)	49, 426
BmgT120I	GGNCC	6 cut(s)	22, 198, 371, 1095, 1405, 1572
BmiI	GGNNCC	3 cut(s)	199, 702, 1476
BmrFI	CCNGG	8 cut(s)	50, 51, 110, 170, 196, 640, 1093, 1576
BmrI	ACTGGG	1 cut(s)	805
BmsI	GCATC	5 cut(s)	736, 997, 1207, 1228, 1462
BmuI	ACTGGG	1 cut(s)	805
BpmI	CTGGAG	2 cut(s)	563, 924
Bpu10I	CCTNAGC	1 cut(s)	134
BpuEI	CTTGAG	4 cut(s)	385, 542, 668, 1529
BpuMI	CCSGG	4 cut(s)	50, 51, 170, 196
BsaAI	YACGTR	1 cut(s)	736
BsaHI	GRCGYC	1 cut(s)	825
BsaI	GGTCTC	2 cut(s)	218, 426
BsaWI	WCCGGW	1 cut(s)	697
BsaXI	ACNNNNNCTCC	2 cut(s)	791, 821
Bse1I	ACTGG	3 cut(s)	800, 907, 1028
Bse21I	CCTNAGG	2 cut(s)	819, 1068
Bse3DI	GCAATG	1 cut(s)	1305
BseBI	CCWGG	4 cut(s)	110, 640, 1093, 1576
BseGI	GGATG	2 cut(s)	45, 63
BseMI	GCAATG	1 cut(s)	1305
BseMII	CTCAG	5 cut(s)	125, 348, 810, 1059, 1521
BseNI	ACTGG	3 cut(s)	800, 907, 1028
BseRI	GAGGAG	1 cut(s)	444
BseX3I	CGGCCG	1 cut(s)	331
BseXI	GCAGC	4 cut(s)	113, 261, 361, 1525
BsgI	GTGCAG	1 cut(s)	268
Bsh1236I	CGCG	1 cut(s)	890
Bsh1285I	CGRYCG	1 cut(s)	334
BshFI	GGCC	4 cut(s)	333, 1066, 1097, 1573
BsiEI	CGRYCG	1 cut(s)	334
BsiHKAI	GWGCWC	2 cut(s)	549, 1421
BsiHKCI	CYCGRG	2 cut(s)	49, 426
BsiSI	CCGG	4 cut(s)	50, 169, 195, 698
BslFI	GGGAC	5 cut(s)	17, 86, 211, 401, 734
BsmAI	GTCTC	3 cut(s)	170, 218, 426
BsmBI	CGTCTC	1 cut(s)	170
BsmFI	GGGAC	5 cut(s)	17, 86, 211, 401, 734
BsmI	GAATGC	3 cut(s)	622, 1023, 1366
BsnI	GGCC	4 cut(s)	333, 1066, 1097, 1573
Bso31I	GGTCTC	2 cut(s)	218, 426
BsoBI	CYCGRG	2 cut(s)	49, 426
Bsp1286I	GDGCHC	2 cut(s)	549, 1421
Bsp143I	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
Bsp19I	CCATGG	1 cut(s)	234
BspACI	CCGC	5 cut(s)	347, 369, 620, 888, 1660
BspANI	GGCC	4 cut(s)	333, 1066, 1097, 1573
BspCNI	CTCAG	5 cut(s)	126, 349, 811, 1060, 1522
BspFNI	CGCG	1 cut(s)	890
BspHI	TCATGA	1 cut(s)	921
BspLI	GGNNCC	3 cut(s)	199, 702, 1476
BspMI	ACCTGC	1 cut(s)	1059
BspPI	GGATC	2 cut(s)	476, 687
BspTNI	GGTCTC	2 cut(s)	218, 426
BsrDI	GCAATG	1 cut(s)	1305
BsrI	ACTGG	3 cut(s)	800, 907, 1028
BssMI	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
BssNI	GRCGYC	1 cut(s)	825
BssT1I	CCWWGG	1 cut(s)	234
Bst2UI	CCWGG	4 cut(s)	110, 640, 1093, 1576
Bst4CI	ACNGT	3 cut(s)	184, 874, 1202
Bst6I	CTCTTC	2 cut(s)	234, 567
BstACI	GRCGYC	1 cut(s)	825
BstAPI	GCANNNNNTGC	1 cut(s)	498
BstBAI	YACGTR	1 cut(s)	736
BstC8I	GCNNGC	1 cut(s)	1465
BstDEI	CTNAG	7 cut(s)	134, 357, 365, 774, 819, 1068, 1530
BstDSI	CCRYGG	3 cut(s)	234, 468, 512
BstENI	CCTNNNNNAGG	1 cut(s)	400
BstF5I	GGATG	2 cut(s)	45, 63
BstFNI	CGCG	1 cut(s)	890
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	2 cut(s)	140, 892
BstKTI	GATC	6 cut(s)	72, 484, 682, 793, 1539, 1584
BstMAI	GTCTC	3 cut(s)	170, 218, 426
BstMBI	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
BstMCI	CGRYCG	1 cut(s)	334
BstMWI	GCNNNNNNNGC	7 cut(s)	135, 498, 553, 1140, 1226, 1350, 1522
BstNI	CCWGG	4 cut(s)	110, 640, 1093, 1576
BstNSI	RCATGY	3 cut(s)	394, 1229, 1486
BstSCI	CCNGG	8 cut(s)	48, 49, 108, 168, 194, 638, 1091, 1574
BstUI	CGCG	1 cut(s)	890
BstV1I	GCAGC	4 cut(s)	113, 261, 361, 1525
BstX2I	RGATCY	2 cut(s)	481, 679
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	2 cut(s)	481, 679
BstZI	CGGCCG	1 cut(s)	331
Bsu36I	CCTNAGG	2 cut(s)	819, 1068
BsuI	GTATCC	3 cut(s)	167, 752, 1190
BsuRI	GGCC	4 cut(s)	333, 1066, 1097, 1573
BtgI	CCRYGG	3 cut(s)	234, 468, 512
BtsCI	GGATG	2 cut(s)	45, 63
BtsIMutI	CAGTG	3 cut(s)	600, 793, 939
BveI	ACCTGC	1 cut(s)	1059
Cac8I	GCNNGC	1 cut(s)	1465
CaiI	CAGNNNCTG	1 cut(s)	1235
CciI	TCATGA	1 cut(s)	921
CfoI	GCGC	2 cut(s)	140, 892
Cfr13I	GGNCC	6 cut(s)	22, 198, 371, 1095, 1405, 1572
Cfr9I	CCCGGG	1 cut(s)	49
CpoI	CGGWCCG	1 cut(s)	371
CseI	GACGC	1 cut(s)	118
Csp6I	GTAC	5 cut(s)	157, 577, 1505, 1586, 1701
CspI	CGGWCCG	1 cut(s)	371
CviAII	CATG	7 cut(s)	235, 391, 877, 922, 1226, 1339, 1483
CviQI	GTAC	5 cut(s)	157, 577, 1505, 1586, 1701
DdeI	CTNAG	7 cut(s)	134, 357, 365, 774, 819, 1068, 1530
DpnI	GATC	6 cut(s)	71, 483, 681, 792, 1538, 1583
DpnII	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
DrdI	GACNNNNNNGTC	1 cut(s)	29
DseDI	GACNNNNNNGTC	1 cut(s)	29
EaeI	YGGCCR	1 cut(s)	331
EagI	CGGCCG	1 cut(s)	331
Eam1104I	CTCTTC	2 cut(s)	234, 567
EarI	CTCTTC	2 cut(s)	234, 567
Ecl136II	GAGCTC	1 cut(s)	547
EclXI	CGGCCG	1 cut(s)	331
Eco130I	CCWWGG	1 cut(s)	234
Eco147I	AGGCCT	1 cut(s)	1066
Eco24I	GRGCYC	1 cut(s)	549
Eco31I	GGTCTC	2 cut(s)	218, 426
Eco47I	GGWCC	4 cut(s)	22, 198, 371, 1405
Eco52I	CGGCCG	1 cut(s)	331
Eco53kI	GAGCTC	1 cut(s)	547
Eco57I	CTGAAG	3 cut(s)	700, 1167, 1422
Eco81I	CCTNAGG	2 cut(s)	819, 1068
Eco88I	CYCGRG	2 cut(s)	49, 426
EcoICRI	GAGCTC	1 cut(s)	547
EcoNI	CCTNNNNNAGG	1 cut(s)	400
EcoRII	CCWGG	4 cut(s)	108, 638, 1091, 1574
EcoT14I	CCWWGG	1 cut(s)	234
EcoT22I	ATGCAT	1 cut(s)	1366
EcoT38I	GRGCYC	1 cut(s)	549
ErhI	CCWWGG	1 cut(s)	234
Esp3I	CGTCTC	1 cut(s)	170
FaeI	CATG	7 cut(s)	238, 394, 880, 925, 1229, 1342, 1486
FalI	AAGNNNNNCTT	2 cut(s)	639, 671
FaqI	GGGAC	5 cut(s)	17, 86, 211, 401, 734
FatI	CATG	7 cut(s)	234, 390, 876, 921, 1225, 1338, 1482
FauNDI	CATATG	1 cut(s)	88
FbaI	TGATCA	2 cut(s)	69, 790
Fnu4HI	GCNGC	5 cut(s)	127, 250, 347, 350, 1514
FokI	GGATG	2 cut(s)	32, 50
FriOI	GRGCYC	1 cut(s)	549
Fsp4HI	GCNGC	5 cut(s)	127, 250, 347, 350, 1514
FspBI	CTAG	3 cut(s)	75, 1350, 1500
GlaI	GCGC	2 cut(s)	139, 891
GluI	GCNGC	5 cut(s)	127, 250, 347, 350, 1514
GsuI	CTGGAG	2 cut(s)	563, 924
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	4 cut(s)	333, 1066, 1097, 1573
HapII	CCGG	4 cut(s)	50, 169, 195, 698
HgaI	GACGC	1 cut(s)	118
HhaI	GCGC	2 cut(s)	140, 892
Hin1I	GRCGYC	1 cut(s)	825
Hin1II	CATG	7 cut(s)	238, 394, 880, 925, 1229, 1342, 1486
Hin6I	GCGC	2 cut(s)	138, 890
HinP1I	GCGC	2 cut(s)	138, 890
HincII	GTYRAC	1 cut(s)	94
HindII	GTYRAC	1 cut(s)	94
HindIII	AAGCTT	2 cut(s)	316, 455
HpaI	GTTAAC	1 cut(s)	94
HpaII	CCGG	4 cut(s)	50, 169, 195, 698
HphI	GGTGA	2 cut(s)	1081, 1214
Hpy166II	GTNNAC	4 cut(s)	94, 1089, 1626, 1703
Hpy188I	TCNGA	3 cut(s)	615, 1078, 1237
Hpy8I	GTNNAC	4 cut(s)	94, 1089, 1626, 1703
Hpy99I	CGWCG	4 cut(s)	223, 830, 1283, 1633
HpyAV	CCTTC	4 cut(s)	593, 894, 956, 1191
HpyCH4III	ACNGT	3 cut(s)	184, 874, 1202
HpyCH4IV	ACGT	7 cut(s)	99, 664, 735, 825, 1278, 1631, 1699
HpyCH4V	TGCA	8 cut(s)	249, 492, 501, 669, 1010, 1229, 1364, 1453
HpyF10VI	GCNNNNNNNGC	7 cut(s)	135, 498, 553, 1140, 1226, 1350, 1522
HpyF3I	CTNAG	7 cut(s)	134, 357, 365, 774, 819, 1068, 1530
HpySE526I	ACGT	7 cut(s)	99, 664, 735, 825, 1278, 1631, 1699
Hsp92I	GRCGYC	1 cut(s)	825
Hsp92II	CATG	7 cut(s)	238, 394, 880, 925, 1229, 1342, 1486
HspAI	GCGC	2 cut(s)	138, 890
Ksp22I	TGATCA	2 cut(s)	69, 790
KspAI	GTTAAC	1 cut(s)	94
Kzo9I	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
LmnI	GCTCC	6 cut(s)	45, 544, 552, 754, 1225, 1424
Lsp1109I	GCAGC	4 cut(s)	113, 261, 361, 1525
LweI	GCATC	5 cut(s)	736, 997, 1207, 1228, 1462
MaeI	CTAG	3 cut(s)	75, 1350, 1500
MaeII	ACGT	7 cut(s)	99, 664, 735, 825, 1278, 1631, 1699
MaeIII	GTNAC	7 cut(s)	266, 410, 440, 779, 937, 1202, 1595
MalI	GATC	6 cut(s)	71, 483, 681, 792, 1538, 1583
MboI	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
MboII	GAAGA	7 cut(s)	251, 584, 823, 937, 971, 1448, 1655
MflI	RGATCY	2 cut(s)	481, 679
MhlI	GDGCHC	2 cut(s)	549, 1421
MluCI	AATT	4 cut(s)	326, 830, 910, 1253
MlyI	GAGTC	4 cut(s)	39, 619, 1226, 1541
MmeI	TCCRAC	2 cut(s)	549, 587
Mph1103I	ATGCAT	1 cut(s)	1366
MseI	TTAA	3 cut(s)	93, 657, 1431
MslI	CAYNNNNRTG	1 cut(s)	875
MspI	CCGG	4 cut(s)	50, 169, 195, 698
MspR9I	CCNGG	8 cut(s)	50, 51, 110, 170, 196, 640, 1093, 1576
Mva1269I	GAATGC	3 cut(s)	622, 1023, 1366
MvaI	CCWGG	4 cut(s)	110, 640, 1093, 1576
MvnI	CGCG	1 cut(s)	890
MwoI	GCNNNNNNNGC	7 cut(s)	135, 498, 553, 1140, 1226, 1350, 1522
NciI	CCSGG	4 cut(s)	50, 51, 170, 196
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	88
NdeII	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
NlaIII	CATG	7 cut(s)	238, 394, 880, 925, 1229, 1342, 1486
NlaIV	GGNNCC	3 cut(s)	199, 702, 1476
NmuCI	GTSAC	5 cut(s)	266, 440, 937, 1202, 1595
NsiI	ATGCAT	1 cut(s)	1366
NspI	RCATGY	3 cut(s)	394, 1229, 1486
PaeR7I	CTCGAG	1 cut(s)	426
PagI	TCATGA	1 cut(s)	921
PasI	CCCWGGG	1 cut(s)	1575
PceI	AGGCCT	1 cut(s)	1066
PcsI	WCGNNNNNNNCGW	3 cut(s)	160, 741, 1275
PctI	GAATGC	3 cut(s)	622, 1023, 1366
PfeI	GAWTC	8 cut(s)	361, 376, 419, 463, 517, 814, 1376, 1652
PflMI	CCANNNNNTGG	2 cut(s)	710, 1386
PfoI	TCCNGGA	1 cut(s)	194
PkrI	GCNGC	5 cut(s)	128, 251, 348, 351, 1515
PleI	GAGTC	4 cut(s)	38, 618, 1226, 1540
PpsI	GAGTC	4 cut(s)	38, 618, 1226, 1540
Ppu21I	YACGTR	1 cut(s)	736
Psp124BI	GAGCTC	1 cut(s)	549
Psp1406I	AACGTT	1 cut(s)	664
Psp6I	CCWGG	4 cut(s)	108, 638, 1091, 1574
PspGI	CCWGG	4 cut(s)	108, 638, 1091, 1574
PspN4I	GGNNCC	3 cut(s)	199, 702, 1476
PspPI	GGNCC	6 cut(s)	22, 198, 371, 1095, 1405, 1572
PspXI	VCTCGAGB	1 cut(s)	426
PstNI	CAGNNNCTG	1 cut(s)	1235
PsuI	RGATCY	2 cut(s)	481, 679
RsaI	GTAC	5 cut(s)	158, 578, 1506, 1587, 1702
RsaNI	GTAC	5 cut(s)	157, 577, 1505, 1586, 1701
RseI	CAYNNNNRTG	1 cut(s)	875
Rsr2I	CGGWCCG	1 cut(s)	371
RsrII	CGGWCCG	1 cut(s)	371
SacI	GAGCTC	1 cut(s)	549
SaqAI	TTAA	3 cut(s)	93, 657, 1431
SatI	GCNGC	5 cut(s)	127, 250, 347, 350, 1514
Sau3AI	GATC	6 cut(s)	69, 481, 679, 790, 1536, 1581
Sau96I	GGNCC	6 cut(s)	22, 198, 371, 1095, 1405, 1572
ScaI	AGTACT	1 cut(s)	1506
SchI	GAGTC	4 cut(s)	39, 619, 1226, 1541
ScrFI	CCNGG	8 cut(s)	50, 51, 110, 170, 196, 640, 1093, 1576
SduI	GDGCHC	2 cut(s)	549, 1421
SfaNI	GCATC	5 cut(s)	736, 997, 1207, 1228, 1462
Sfr274I	CTCGAG	1 cut(s)	426
SinI	GGWCC	4 cut(s)	22, 198, 371, 1405
SlaI	CTCGAG	1 cut(s)	426
SmaI	CCCGGG	1 cut(s)	51
SmiMI	CAYNNNNRTG	1 cut(s)	875
SmlI	CTYRAG	5 cut(s)	400, 426, 557, 683, 1508
SmoI	CTYRAG	5 cut(s)	400, 426, 557, 683, 1508
SpeI	ACTAGT	1 cut(s)	74
Sse9I	AATT	4 cut(s)	326, 830, 910, 1253
SseBI	AGGCCT	1 cut(s)	1066
SsiI	CCGC	5 cut(s)	347, 369, 620, 888, 1660
SspMI	CTAG	3 cut(s)	75, 1350, 1500
SstI	GAGCTC	1 cut(s)	549
StuI	AGGCCT	1 cut(s)	1066
StyD4I	CCNGG	8 cut(s)	48, 49, 108, 168, 194, 638, 1091, 1574
StyI	CCWWGG	1 cut(s)	234
TaaI	ACNGT	3 cut(s)	184, 874, 1202
TaiI	ACGT	7 cut(s)	102, 667, 738, 828, 1281, 1634, 1702
TaqI	TCGA	7 cut(s)	321, 427, 744, 828, 1281, 1535, 1650
TaqII	GACCGA	3 cut(s)	215, 388, 1422
TasI	AATT	4 cut(s)	326, 830, 910, 1253
TatI	WGTACW	2 cut(s)	576, 1504
TauI	GCSGC	1 cut(s)	349
TfiI	GAWTC	8 cut(s)	361, 376, 419, 463, 517, 814, 1376, 1652
Tru1I	TTAA	3 cut(s)	93, 657, 1431
Tru9I	TTAA	3 cut(s)	93, 657, 1431
TscAI	CASTG	3 cut(s)	600, 800, 946
TseFI	GTSAC	5 cut(s)	266, 440, 937, 1202, 1595
TseI	GCWGC	4 cut(s)	126, 249, 349, 1513
Tsp45I	GTSAC	5 cut(s)	266, 440, 937, 1202, 1595
TspDTI	ATGAA	4 cut(s)	910, 938, 972, 1138
TspGWI	ACGGA	3 cut(s)	236, 485, 529
TspMI	CCCGGG	1 cut(s)	49
TspRI	CASTG	3 cut(s)	600, 800, 946
Van91I	CCANNNNNTGG	2 cut(s)	710, 1386
VpaK11BI	GGWCC	4 cut(s)	22, 198, 371, 1405
XagI	CCTNNNNNAGG	1 cut(s)	400
XceI	RCATGY	3 cut(s)	394, 1229, 1486
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1035
XhoI	CTCGAG	1 cut(s)	426
XmaI	CCCGGG	1 cut(s)	49
XspI	CTAG	3 cut(s)	75, 1350, 1500
ZraI	GACGTC	1 cut(s)	826
ZrmI	AGTACT	1 cut(s)	1506
Zsp2I	ATGCAT	1 cut(s)	1366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.