Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 58 |
| AccB1I | GGYRCC | 2 cut(s) | 58, 125 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 940 |
| AccI | GTMKAC | 1 cut(s) | 309 |
| AccII | CGCG | 2 cut(s) | 183, 1556 |
| AciI | CCGC | 5 cut(s) | 21, 183, 510, 1193, 1322 |
| AclWI | GGATC | 5 cut(s) | 575, 588, 709, 1303, 1383 |
| AcoI | YGGCCR | 2 cut(s) | 608, 1160 |
| AcsI | RAATTY | 8 cut(s) | 273, 338, 372, 674, 812, 1089, 1240, 1848 |
| AcuI | CTGAAG | 2 cut(s) | 677, 1830 |
| AdeI | CACNNNGTG | 1 cut(s) | 876 |
| AfaI | GTAC | 5 cut(s) | 60, 188, 262, 932, 985 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 62, 122, 481, 787, 940, 1077, 1165 |
| AflII | CTTAAG | 3 cut(s) | 575, 755, 782 |
| AflIII | ACRYGT | 1 cut(s) | 1369 |
| AgsI | TTSAA | 7 cut(s) | 391, 562, 698, 725, 1658, 1726, 1735 |
| AjiI | CACGTC | 1 cut(s) | 351 |
| Alw21I | GWGCWC | 1 cut(s) | 648 |
| Alw26I | GTCTC | 3 cut(s) | 230, 617, 1174 |
| AlwI | GGATC | 5 cut(s) | 575, 588, 709, 1303, 1383 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 439 |
| AoxI | GGCC | 2 cut(s) | 608, 1160 |
| ApeKI | GCWGC | 3 cut(s) | 52, 436, 1606 |
| ApoI | RAATTY | 8 cut(s) | 273, 338, 372, 674, 812, 1089, 1240, 1848 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1475 |
| Asp718I | GGTACC | 1 cut(s) | 58 |
| AspLEI | GCGC | 1 cut(s) | 850 |
| AspS9I | GGNCC | 1 cut(s) | 778 |
| AsuC2I | CCSGG | 3 cut(s) | 63, 915, 1253 |
| AsuHPI | GGTGA | 1 cut(s) | 110 |
| AsuNHI | GCTAGC | 1 cut(s) | 1150 |
| AvaII | GGWCC | 1 cut(s) | 778 |
| BaeGI | GKGCMC | 1 cut(s) | 94 |
| BamHI | GGATCC | 1 cut(s) | 580 |
| BanI | GGYRCC | 2 cut(s) | 58, 125 |
| BanII | GRGCYC | 2 cut(s) | 648, 713 |
| BauI | CACGAG | 1 cut(s) | 525 |
| Bbv12I | GWGCWC | 1 cut(s) | 648 |
| BbvI | GCAGC | 3 cut(s) | 39, 448, 1618 |
| BccI | CCATC | 2 cut(s) | 137, 1685 |
| BceAI | ACGGC | 2 cut(s) | 34, 623 |
| BcnI | CCSGG | 3 cut(s) | 63, 915, 1253 |
| BcoDI | GTCTC | 3 cut(s) | 230, 617, 1174 |
| BfaI | CTAG | 6 cut(s) | 246, 479, 503, 585, 641, 1151 |
| BfmI | CTRYAG | 1 cut(s) | 1533 |
| BfrI | CTTAAG | 3 cut(s) | 575, 755, 782 |
| BisI | GCNGC | 4 cut(s) | 53, 437, 511, 1607 |
| BlsI | GCNGC | 4 cut(s) | 54, 438, 512, 1608 |
| Bme1390I | CCNGG | 3 cut(s) | 63, 915, 1253 |
| Bme18I | GGWCC | 1 cut(s) | 778 |
| BmgBI | CACGTC | 1 cut(s) | 351 |
| BmgT120I | GGNCC | 1 cut(s) | 778 |
| BmiI | GGNNCC | 8 cut(s) | 60, 127, 582, 710, 779, 997, 1569, 1763 |
| BmrFI | CCNGG | 3 cut(s) | 63, 915, 1253 |
| BmsI | GCATC | 1 cut(s) | 835 |
| BmtI | GCTAGC | 1 cut(s) | 1154 |
| BoxI | GACNNNNGTC | 1 cut(s) | 349 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1028, 1797 |
| BpuEI | CTTGAG | 1 cut(s) | 1263 |
| BpuMI | CCSGG | 3 cut(s) | 63, 915, 1253 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1294 |
| BsaI | GGTCTC | 1 cut(s) | 1174 |
| BsaJI | CCNNGG | 5 cut(s) | 121, 913, 1125, 1392, 1673 |
| BsaWI | WCCGGW | 1 cut(s) | 998 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 340, 370, 887, 917 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 62, 122, 481, 787, 940, 1077, 1165 |
| Bse118I | RCCGGY | 3 cut(s) | 55, 474, 1158 |
| Bse1I | ACTGG | 4 cut(s) | 934, 1443, 1474, 1765 |
| Bse3DI | GCAATG | 4 cut(s) | 1105, 1266, 1437, 1459 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1294 |
| BseDI | CCNNGG | 5 cut(s) | 121, 913, 1125, 1392, 1673 |
| BseGI | GGATG | 1 cut(s) | 1657 |
| BseJI | GATNNNNATC | 1 cut(s) | 1294 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 62, 122, 481, 787, 940, 1077, 1165 |
| BseMI | GCAATG | 4 cut(s) | 1105, 1266, 1437, 1459 |
| BseMII | CTCAG | 4 cut(s) | 225, 561, 1209, 1818 |
| BseNI | ACTGG | 4 cut(s) | 934, 1443, 1474, 1765 |
| BseRI | GAGGAG | 2 cut(s) | 348, 543 |
| BseSI | GKGCMC | 1 cut(s) | 94 |
| BseXI | GCAGC | 3 cut(s) | 39, 448, 1618 |
| BsgI | GTGCAG | 1 cut(s) | 507 |
| Bsh1236I | CGCG | 2 cut(s) | 183, 1556 |
| Bsh1285I | CGRYCG | 1 cut(s) | 460 |
| BshFI | GGCC | 2 cut(s) | 610, 1162 |
| BshNI | GGYRCC | 2 cut(s) | 58, 125 |
| BsiEI | CGRYCG | 1 cut(s) | 460 |
| BsiHKAI | GWGCWC | 1 cut(s) | 648 |
| BsiSI | CCGG | 7 cut(s) | 56, 63, 475, 915, 999, 1159, 1252 |
| BslFI | GGGAC | 4 cut(s) | 223, 238, 791, 1720 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 62, 122, 481, 787, 940, 1077, 1165 |
| BsmAI | GTCTC | 3 cut(s) | 230, 617, 1174 |
| BsmBI | CGTCTC | 1 cut(s) | 617 |
| BsmFI | GGGAC | 4 cut(s) | 223, 238, 791, 1720 |
| BsnI | GGCC | 2 cut(s) | 610, 1162 |
| Bso31I | GGTCTC | 1 cut(s) | 1174 |
| Bsp1286I | GDGCHC | 3 cut(s) | 94, 648, 713 |
| Bsp143I | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| Bsp19I | CCATGG | 1 cut(s) | 1392 |
| BspACI | CCGC | 5 cut(s) | 21, 183, 510, 1193, 1322 |
| BspANI | GGCC | 2 cut(s) | 610, 1162 |
| BspCNI | CTCAG | 4 cut(s) | 226, 562, 1210, 1819 |
| BspFNI | CGCG | 2 cut(s) | 183, 1556 |
| BspLI | GGNNCC | 8 cut(s) | 60, 127, 582, 710, 779, 997, 1569, 1763 |
| BspMAI | CTGCAG | 1 cut(s) | 1537 |
| BspOI | GCTAGC | 1 cut(s) | 1154 |
| BspPI | GGATC | 5 cut(s) | 575, 588, 709, 1303, 1383 |
| BspQI | GCTCTTC | 1 cut(s) | 1016 |
| BspT107I | GGYRCC | 2 cut(s) | 58, 125 |
| BspTI | CTTAAG | 3 cut(s) | 575, 755, 782 |
| BspTNI | GGTCTC | 1 cut(s) | 1174 |
| BsrDI | GCAATG | 4 cut(s) | 1105, 1266, 1437, 1459 |
| BsrFI | RCCGGY | 3 cut(s) | 55, 474, 1158 |
| BsrI | ACTGG | 4 cut(s) | 934, 1443, 1474, 1765 |
| BssAI | RCCGGY | 3 cut(s) | 55, 474, 1158 |
| BssECI | CCNNGG | 5 cut(s) | 121, 913, 1125, 1392, 1673 |
| BssMI | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| BssSI | CACGAG | 1 cut(s) | 525 |
| BssT1I | CCWWGG | 3 cut(s) | 121, 1392, 1673 |
| Bst2BI | CACGAG | 1 cut(s) | 525 |
| Bst4CI | ACNGT | 3 cut(s) | 173, 970, 1180 |
| Bst6I | CTCTTC | 1 cut(s) | 1016 |
| BstAFI | CTTAAG | 3 cut(s) | 575, 755, 782 |
| BstC8I | GCNNGC | 4 cut(s) | 1152, 1416, 1558, 1562 |
| BstDEI | CTNAG | 8 cut(s) | 146, 234, 570, 743, 1028, 1218, 1797, 1827 |
| BstDSI | CCRYGG | 1 cut(s) | 1392 |
| BstF5I | GGATG | 1 cut(s) | 1657 |
| BstFNI | CGCG | 2 cut(s) | 183, 1556 |
| BstHHI | GCGC | 1 cut(s) | 850 |
| BstKTI | GATC | 9 cut(s) | 10, 460, 535, 583, 704, 1263, 1298, 1378, 1501 |
| BstMAI | GTCTC | 3 cut(s) | 230, 617, 1174 |
| BstMBI | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| BstMCI | CGRYCG | 1 cut(s) | 460 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 27, 602, 1319, 1606, 1615 |
| BstNSI | RCATGY | 3 cut(s) | 456, 600, 1373 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 349 |
| BstSCI | CCNGG | 3 cut(s) | 61, 913, 1251 |
| BstSFI | CTRYAG | 1 cut(s) | 1533 |
| BstSLI | GKGCMC | 1 cut(s) | 94 |
| BstUI | CGCG | 2 cut(s) | 183, 1556 |
| BstV1I | GCAGC | 3 cut(s) | 39, 448, 1618 |
| BstX2I | RGATCY | 3 cut(s) | 580, 701, 1295 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1772 |
| BstYI | RGATCY | 3 cut(s) | 580, 701, 1295 |
| BsuRI | GGCC | 2 cut(s) | 610, 1162 |
| BtgI | CCRYGG | 1 cut(s) | 1392 |
| BtgZI | GCGATG | 1 cut(s) | 778 |
| BtrI | CACGTC | 1 cut(s) | 351 |
| BtsCI | GGATG | 1 cut(s) | 1657 |
| BtsIMutI | CAGTG | 5 cut(s) | 542, 1013, 1176, 1418, 1450 |
| Cac8I | GCNNGC | 4 cut(s) | 1152, 1416, 1558, 1562 |
| CaiI | CAGNNNCTG | 1 cut(s) | 439 |
| CfoI | GCGC | 1 cut(s) | 850 |
| Cfr10I | RCCGGY | 3 cut(s) | 55, 474, 1158 |
| Cfr13I | GGNCC | 1 cut(s) | 778 |
| CseI | GACGC | 1 cut(s) | 354 |
| Csp6I | GTAC | 5 cut(s) | 59, 187, 261, 931, 984 |
| CviQI | GTAC | 5 cut(s) | 59, 187, 261, 931, 984 |
| DdeI | CTNAG | 8 cut(s) | 146, 234, 570, 743, 1028, 1218, 1797, 1827 |
| DpnI | GATC | 9 cut(s) | 9, 459, 534, 582, 703, 1262, 1297, 1377, 1500 |
| DpnII | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| DraIII | CACNNNGTG | 1 cut(s) | 876 |
| EaeI | YGGCCR | 2 cut(s) | 608, 1160 |
| Eam1104I | CTCTTC | 1 cut(s) | 1016 |
| EarI | CTCTTC | 1 cut(s) | 1016 |
| EciI | GGCGGA | 2 cut(s) | 1208, 1337 |
| Ecl136II | GAGCTC | 1 cut(s) | 646 |
| Eco130I | CCWWGG | 3 cut(s) | 121, 1392, 1673 |
| Eco24I | GRGCYC | 2 cut(s) | 648, 713 |
| Eco31I | GGTCTC | 1 cut(s) | 1174 |
| Eco47I | GGWCC | 1 cut(s) | 778 |
| Eco53kI | GAGCTC | 1 cut(s) | 646 |
| Eco57I | CTGAAG | 2 cut(s) | 677, 1830 |
| EcoICRI | GAGCTC | 1 cut(s) | 646 |
| EcoO109I | RGGNCCY | 1 cut(s) | 778 |
| EcoT14I | CCWWGG | 3 cut(s) | 121, 1392, 1673 |
| EcoT22I | ATGCAT | 2 cut(s) | 617, 1459 |
| EcoT38I | GRGCYC | 2 cut(s) | 648, 713 |
| ErhI | CCWWGG | 3 cut(s) | 121, 1392, 1673 |
| Esp3I | CGTCTC | 1 cut(s) | 617 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1657, 1689 |
| FaqI | GGGAC | 4 cut(s) | 223, 238, 791, 1720 |
| FauI | CCCGC | 2 cut(s) | 14, 176 |
| FauNDI | CATATG | 1 cut(s) | 203 |
| FblI | GTMKAC | 1 cut(s) | 309 |
| Fnu4HI | GCNGC | 4 cut(s) | 53, 437, 511, 1607 |
| FokI | GGATG | 1 cut(s) | 1664 |
| FriOI | GRGCYC | 2 cut(s) | 648, 713 |
| Fsp4HI | GCNGC | 4 cut(s) | 53, 437, 511, 1607 |
| FspBI | CTAG | 6 cut(s) | 246, 479, 503, 585, 641, 1151 |
| GlaI | GCGC | 1 cut(s) | 849 |
| GluI | GCNGC | 4 cut(s) | 53, 437, 511, 1607 |
| HaeIII | GGCC | 2 cut(s) | 610, 1162 |
| HapII | CCGG | 7 cut(s) | 56, 63, 475, 915, 999, 1159, 1252 |
| HgaI | GACGC | 1 cut(s) | 354 |
| HhaI | GCGC | 1 cut(s) | 850 |
| Hin6I | GCGC | 1 cut(s) | 848 |
| HinP1I | GCGC | 1 cut(s) | 848 |
| HincII | GTYRAC | 2 cut(s) | 106, 354 |
| HindII | GTYRAC | 2 cut(s) | 106, 354 |
| HindIII | AAGCTT | 2 cut(s) | 1311, 1503 |
| HinfI | GANTC | 6 cut(s) | 295, 518, 562, 1325, 1359, 1539 |
| HpaII | CCGG | 7 cut(s) | 56, 63, 475, 915, 999, 1159, 1252 |
| HphI | GGTGA | 1 cut(s) | 110 |
| Hpy166II | GTNNAC | 4 cut(s) | 106, 160, 310, 354 |
| Hpy188I | TCNGA | 7 cut(s) | 16, 48, 344, 421, 1047, 1129, 1174 |
| Hpy188III | TCNNGA | 6 cut(s) | 391, 641, 1307, 1480, 1525, 1773 |
| Hpy8I | GTNNAC | 4 cut(s) | 106, 160, 310, 354 |
| HpyAV | CCTTC | 8 cut(s) | 20, 188, 479, 652, 692, 743, 1393, 1590 |
| HpyCH4III | ACNGT | 3 cut(s) | 173, 970, 1180 |
| HpyCH4IV | ACGT | 1 cut(s) | 350 |
| HpyCH4V | TGCA | 9 cut(s) | 230, 524, 615, 1108, 1271, 1430, 1457, 1535, 1644 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 27, 602, 1319, 1606, 1615 |
| HpyF3I | CTNAG | 8 cut(s) | 146, 234, 570, 743, 1028, 1218, 1797, 1827 |
| HpySE526I | ACGT | 1 cut(s) | 350 |
| HspAI | GCGC | 1 cut(s) | 848 |
| KpnI | GGTACC | 1 cut(s) | 62 |
| Kzo9I | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| LguI | GCTCTTC | 1 cut(s) | 1016 |
| LmnI | GCTCC | 4 cut(s) | 651, 708, 1761, 1837 |
| Lsp1109I | GCAGC | 3 cut(s) | 39, 448, 1618 |
| LweI | GCATC | 1 cut(s) | 835 |
| MaeI | CTAG | 6 cut(s) | 246, 479, 503, 585, 641, 1151 |
| MaeII | ACGT | 1 cut(s) | 350 |
| MaeIII | GTNAC | 3 cut(s) | 278, 1033, 1694 |
| MalI | GATC | 9 cut(s) | 9, 459, 534, 582, 703, 1262, 1297, 1377, 1500 |
| MboI | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| MboII | GAAGA | 6 cut(s) | 683, 919, 1033, 1255, 1541, 1754 |
| MflI | RGATCY | 3 cut(s) | 580, 701, 1295 |
| MhlI | GDGCHC | 3 cut(s) | 94, 648, 713 |
| MlyI | GAGTC | 1 cut(s) | 1533 |
| MmeI | TCCRAC | 1 cut(s) | 590 |
| Mph1103I | ATGCAT | 2 cut(s) | 617, 1459 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1475 |
| MseI | TTAA | 4 cut(s) | 576, 756, 783, 1248 |
| MslI | CAYNNNNRTG | 4 cut(s) | 610, 736, 971, 1370 |
| MspA1I | CMGCKG | 1 cut(s) | 439 |
| MspCI | CTTAAG | 3 cut(s) | 575, 755, 782 |
| MspI | CCGG | 7 cut(s) | 56, 63, 475, 915, 999, 1159, 1252 |
| MspR9I | CCNGG | 3 cut(s) | 63, 915, 1253 |
| MvnI | CGCG | 2 cut(s) | 183, 1556 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 27, 602, 1319, 1606, 1615 |
| NciI | CCSGG | 3 cut(s) | 63, 915, 1253 |
| NcoI | CCATGG | 1 cut(s) | 1392 |
| NdeI | CATATG | 1 cut(s) | 203 |
| NdeII | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| NheI | GCTAGC | 1 cut(s) | 1150 |
| NlaIV | GGNNCC | 8 cut(s) | 60, 127, 582, 710, 779, 997, 1569, 1763 |
| NmuCI | GTSAC | 2 cut(s) | 278, 1694 |
| NsiI | ATGCAT | 2 cut(s) | 617, 1459 |
| NspI | RCATGY | 3 cut(s) | 456, 600, 1373 |
| PciI | ACATGT | 1 cut(s) | 1369 |
| PciSI | GCTCTTC | 1 cut(s) | 1016 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 318 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1475 |
| PfeI | GAWTC | 5 cut(s) | 295, 518, 562, 1325, 1359 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 940 |
| PkrI | GCNGC | 4 cut(s) | 54, 438, 512, 1608 |
| Ple19I | CGATCG | 1 cut(s) | 460 |
| PleI | GAGTC | 1 cut(s) | 1533 |
| PpsI | GAGTC | 1 cut(s) | 1533 |
| PpuMI | RGGWCCY | 1 cut(s) | 778 |
| PscI | ACATGT | 1 cut(s) | 1369 |
| PshAI | GACNNNNGTC | 1 cut(s) | 349 |
| Psp124BI | GAGCTC | 1 cut(s) | 648 |
| Psp5II | RGGWCCY | 1 cut(s) | 778 |
| PspN4I | GGNNCC | 8 cut(s) | 60, 127, 582, 710, 779, 997, 1569, 1763 |
| PspPI | GGNCC | 1 cut(s) | 778 |
| PspPPI | RGGWCCY | 1 cut(s) | 778 |
| PstI | CTGCAG | 1 cut(s) | 1537 |
| PstNI | CAGNNNCTG | 1 cut(s) | 439 |
| PsuI | RGATCY | 3 cut(s) | 580, 701, 1295 |
| PvuI | CGATCG | 1 cut(s) | 460 |
| PvuII | CAGCTG | 1 cut(s) | 439 |
| RsaI | GTAC | 5 cut(s) | 60, 188, 262, 932, 985 |
| RsaNI | GTAC | 5 cut(s) | 59, 187, 261, 931, 984 |
| RseI | CAYNNNNRTG | 4 cut(s) | 610, 736, 971, 1370 |
| SacI | GAGCTC | 1 cut(s) | 648 |
| SapI | GCTCTTC | 1 cut(s) | 1016 |
| SaqAI | TTAA | 4 cut(s) | 576, 756, 783, 1248 |
| SatI | GCNGC | 4 cut(s) | 53, 437, 511, 1607 |
| Sau3AI | GATC | 9 cut(s) | 7, 457, 532, 580, 701, 1260, 1295, 1375, 1498 |
| Sau96I | GGNCC | 1 cut(s) | 778 |
| SchI | GAGTC | 1 cut(s) | 1533 |
| ScrFI | CCNGG | 3 cut(s) | 63, 915, 1253 |
| SduI | GDGCHC | 3 cut(s) | 94, 648, 713 |
| SfaNI | GCATC | 1 cut(s) | 835 |
| SfcI | CTRYAG | 1 cut(s) | 1533 |
| SinI | GGWCC | 1 cut(s) | 778 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 610, 736, 971, 1370 |
| SmlI | CTYRAG | 4 cut(s) | 575, 755, 782, 1278 |
| SmoI | CTYRAG | 4 cut(s) | 575, 755, 782, 1278 |
| SsiI | CCGC | 5 cut(s) | 21, 183, 510, 1193, 1322 |
| SspI | AATATT | 1 cut(s) | 1683 |
| SspMI | CTAG | 6 cut(s) | 246, 479, 503, 585, 641, 1151 |
| SstI | GAGCTC | 1 cut(s) | 648 |
| StyD4I | CCNGG | 3 cut(s) | 61, 913, 1251 |
| StyI | CCWWGG | 3 cut(s) | 121, 1392, 1673 |
| TaaI | ACNGT | 3 cut(s) | 173, 970, 1180 |
| TaiI | ACGT | 1 cut(s) | 353 |
| TaqI | TCGA | 3 cut(s) | 166, 321, 807 |
| TatI | WGTACW | 1 cut(s) | 260 |
| TauI | GCSGC | 1 cut(s) | 513 |
| TfiI | GAWTC | 5 cut(s) | 295, 518, 562, 1325, 1359 |
| Tru1I | TTAA | 4 cut(s) | 576, 756, 783, 1248 |
| Tru9I | TTAA | 4 cut(s) | 576, 756, 783, 1248 |
| TscAI | CASTG | 5 cut(s) | 542, 1020, 1183, 1425, 1450 |
| TseFI | GTSAC | 2 cut(s) | 278, 1694 |
| TseI | GCWGC | 3 cut(s) | 52, 436, 1606 |
| Tsp45I | GTSAC | 2 cut(s) | 278, 1694 |
| TspDTI | ATGAA | 9 cut(s) | 287, 928, 1034, 1068, 1234, 1255, 1564, 1572, 1755 |
| TspGWI | ACGGA | 1 cut(s) | 423 |
| TspRI | CASTG | 5 cut(s) | 542, 1020, 1183, 1425, 1450 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 940 |
| Vha464I | CTTAAG | 3 cut(s) | 575, 755, 782 |
| VpaK11BI | GGWCC | 1 cut(s) | 778 |
| XapI | RAATTY | 8 cut(s) | 273, 338, 372, 674, 812, 1089, 1240, 1848 |
| XbaI | TCTAGA | 1 cut(s) | 640 |
| XceI | RCATGY | 3 cut(s) | 456, 600, 1373 |
| XmiI | GTMKAC | 1 cut(s) | 309 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1475 |
| XspI | CTAG | 6 cut(s) | 246, 479, 503, 585, 641, 1151 |
| Zsp2I | ATGCAT | 2 cut(s) | 617, 1459 |